##-- file opened 2019-07-28T04:09:50.819Z @21:09:51.25 -> showStatus @21:09:53.26 -> Cmd.loadCartridge -4 @21:09:53.39 Gate.power :main,:ON,limit to 5000mA @21:09:53.40 Gate.power :analytic1,:ON,limit to 5000mA @21:09:53.43 Gate.power :analytic2,:ON,limit to 5000mA @21:09:53.45 Gate.power :analytic3,:ON,limit to 5000mA @21:09:53.58 Powering core bus @21:09:55.87 SPR Module 1, FW Rev. B, FPGA Rev. C @21:09:55.99 Archive.configure :default @21:09:56.11 Air.configure :default @21:09:56.21 Lysis1.configure :default @21:09:56.32 Lysis2.configure :default @21:09:56.44 Plunge.configure :default @21:09:56.55 SamplePump.configure :default @21:09:56.67 ExtraPump.configure :default @21:09:56.79 Toroid.configure :default @21:09:56.91 Carousel.configure :default @21:09:57.03 Pressure.configure :default @21:09:57.13 PinPort.configure :default @21:09:57.18 All elf controllers running firmware version 4.58 @21:09:57.21 Gate.power :motor,:ON,limit to 5000mA @21:09:57.33 Archive.reconfigure :default @21:09:57.65 Archive.home.jog 0 @21:09:57.78 Toroid.reconfigure :default @21:09:57.86 Plunge.reconfigure :default @21:09:57.89 Lysis2.reconfigure :default @21:09:57.93 Lysis1.reconfigure :default @21:09:57.98 PinPort.reconfigure :default @21:09:58.04 Air.reconfigure :default @21:09:58.25 Plunge.home.jog 0 @21:09:58.30 Lysis1.home.jog 0 @21:09:58.36 Lysis2.home.jog 0 @21:09:58.42 PinPort.home.jog 0 @21:09:58.47 Toroid.home.jog 0 @21:09:58.53 Air.home.jog 0 @21:09:58.78 Archive.reconfigure :homing @21:09:58.99 Toroid.reconfigure :homing @21:09:59.04 Plunge.reconfigure :homing @21:09:59.09 PinPort.reconfigure :homing @21:09:59.13 Lysis2.reconfigure :homing @21:09:59.18 Lysis1.reconfigure :homing @21:09:59.37 Lysis1.jog 1407 @21:09:59.42 Archive.jog 1395 @21:09:59.48 Plunge.jog 1558 @21:09:59.53 Lysis2.jog 162 @21:09:59.59 PinPort.jog -107 @21:09:59.64 Toroid.jog 630 @21:09:59.73 Air.reconfigure :homing @21:09:59.81 PinPort.setRawPosition! 0 @21:09:59.86 PinPort.seek :home @21:09:59.93 Air.jog 1413 @21:10:00.03 PinPort.reconfigure :default @21:10:00.05 Lysis2.setRawPosition! 50 @21:10:00.11 Lysis2.seek :home @21:10:00.41 Lysis2.reconfigure :default @21:10:00.53 Lysis1.setRawPosition! 50 @21:10:00.59 Lysis1.seek :home @21:10:00.67 Archive.setRawPosition! 50 @21:10:00.72 Archive.seek :home @21:10:00.80 Plunge.setRawPosition! 50 @21:10:00.84 Plunge.seek :home @21:10:00.94 Lysis1.reconfigure :default @21:10:01.03 Archive.reconfigure :default @21:10:01.13 Air.setRawPosition! 50 @21:10:01.18 Air.seek :home @21:10:01.27 Plunge.reconfigure :default @21:10:01.47 Air.reconfigure :default @21:10:01.79 Toroid.setRawPosition! 0 @21:10:01.83 Toroid.seek :home @21:10:01.92 Toroid.reconfigure :default @21:10:02.05 Toroid.seek :clear @21:10:03.67 Carousel.reconfigure :default @21:10:03.74 Carousel.home.jog 0 @21:10:04.23 Carousel.seek 15 @21:10:04.38 Selecting Cartridge 12 @21:10:04.63 Carousel.seek 12 @21:10:05.52 Lysis1.seek :locked @21:10:09.65 Plunge.seek :locked @21:10:09.77 Air.seek :locked @21:10:13.91 Powering cartridge bus @21:10:16.03 Gate.setIR 1750,5000,0,6 @21:10:16.16 Gate.setIR 0,5000,0,6 @21:10:16.20 Loaded Archive Cartridge Gate.power :cartridge,:OFF @21:10:31.72 -> Cmd.startFiltering @21:10:31.95 Duration of filtering limited to 2:05:00 @21:10:32.20 Priming sample loop w/100ml, bypass w/10ml @21:10:32.21 SamplePump.setPosition! 0ml @21:10:32.26 Bypass.open @21:10:33.75 Intake.open @21:10:34.59 Sea 2.8m deep (18.9psia) @21:10:34.61 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:10:37.47 Vacuum tare is 52% of full scale @21:10:37.59 Pressure.reconfigure :default @21:10:37.65 Pressure.hold @21:10:38.18 SamplePump.reconfigure :prime @21:10:38.26 SamplePump.seek 10ml,1:21 @21:10:47.22 Pressure.coast @21:10:47.27 SamplePump.setPosition! 0ml @21:10:47.31 Exhaust.open @21:10:48.74 Bypass.close @21:10:50.27 Pressure.reconfigure :default @21:10:50.33 Pressure.hold @21:10:50.44 Sea 2.7m deep (18.6psia) @21:10:50.57 SamplePump.reconfigure :prime @21:10:50.64 SamplePump.seek 100ml,8:51 @21:12:46.06 Pressure.coast @21:12:46.12 Bypass.open @21:12:46.82 Intake.close @21:12:47.65 Toroid.reconfigure :hold @21:12:47.71 Toroid.seek :crack @21:12:48.51 Toroid.reconfigure :default @21:12:48.57 Toroid.seek :clear @21:12:50.13 Toroid.reconfigure :hold @21:12:50.20 Toroid.seek :cartridge @21:12:52.01 Toroid.reconfigure :default @21:12:52.07 Toroid.seek :clear @21:12:54.04 Pressurizing cartridge to 26psi above 18.2psia @21:12:54.06 SamplePump.setPosition! 0ml @21:12:54.10 Intake.open @21:12:54.81 Bypass.close @21:12:55.51 Exhaust.close @21:12:56.32 Pressure.reconfigure :default @21:12:56.38 Pressure.hold @21:12:56.54 SamplePump.reconfigure :charge @21:12:56.61 SamplePump.seek 2.3ml,42 seconds @21:13:03.90 Pressure.coast @21:13:03.96 Intake.close @21:13:04.75 Pressurized cartridge with 0.247ml to 26.2psi @21:13:04.76 Isolated @40.7psia (22.4psi) @21:13:04.77 Checking for cartridge leaks from 40.7psia @21:13:04.80 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @21:13:09.91 Sampling 1000.0ml @21:13:09.92 SamplePump.setPosition! 0ml @21:13:09.97 Exhaust.open @21:13:10.68 Intake.open @21:13:11.51 Pressure.reconfigure :default @21:13:11.57 Pressure.hold @21:13:11.68 Sea 2.6m deep (18.6psia) @21:13:11.81 SamplePump.reconfigure :sample @21:13:11.89 SamplePump.seek 1000ml,1:23:53 @21:13:14.55 Cartridge 12 sampling 1/1000ml at 0.39ml/s with 21.8psi vacuum @21:14:14.55 Cartridge 12 sampling 23/1000ml at 0.37ml/s with 22.0psi vacuum @21:15:14.55 Cartridge 12 sampling 45/1000ml at 0.37ml/s with 24.0psi vacuum @21:16:14.55 Cartridge 12 sampling 65/1000ml at 0.35ml/s with 20.8psi vacuum @21:17:14.55 Cartridge 12 sampling 84/1000ml at 0.26ml/s with 22.0psi vacuum @21:18:14.55 Cartridge 12 sampling 101/1000ml at 0.31ml/s with 25.4psi vacuum 1.6m deep (17.0psia) @21:19:14.55 Cartridge 12 sampling 118/1000ml at 0.28ml/s with 22.5psi vacuum @21:20:14.55 Cartridge 12 sampling 134/1000ml at 0.27ml/s with 22.9psi vacuum @21:21:14.55 Cartridge 12 sampling 150/1000ml at 0.28ml/s with 26.7psi vacuum @21:22:14.55 Cartridge 12 sampling 166/1000ml at 0.24ml/s with 26.2psi vacuum @21:23:14.55 Cartridge 12 sampling 182/1000ml at 0.23ml/s with 25.2psi vacuum @21:24:14.55 Cartridge 12 sampling 198/1000ml at 0.22ml/s with 25.9psi vacuum @21:25:14.55 Cartridge 12 sampling 213/1000ml at 0.22ml/s with 25.7psi vacuum @21:26:14.55 Cartridge 12 sampling 227/1000ml at 0.22ml/s with 28.0psi vacuum @21:27:14.55 Cartridge 12 sampling 241/1000ml at 0.21ml/s with 26.5psi vacuum @21:27:19.93 Flow averaged 0.29ml/s over 14:03 @21:27:19.96 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @21:27:20.66 Relieved excess vacuum in 6 seconds @21:27:20.68 SamplePump.seek 1000ml,1:03:42 @21:27:22.51 Flow estimate reduced to 0.23ml/s @21:27:22.54 Waiting up to 15 seconds for vacuum to reduce from 29.0 to 18.0psi @21:27:23.54 Relieved excess vacuum in 2.7 seconds @21:27:23.56 SamplePump.seek 1000ml,1:03:41 @21:27:25.39 Flow estimate reduced to 0.18ml/s @21:27:25.41 Intake.close @21:27:25.46 Exhaust.close @21:27:25.65 Toroid.reconfigure :hold @21:27:25.72 Toroid.seek :bypass @21:27:27.34 Toroid.reconfigure :default @21:27:27.41 Toroid.seek :clear @21:27:29.03 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @21:27:29.05 Pressure.coast @21:27:29.23 Sampled 242.7ml @21:27:31.50 -> Cmd.startProcessing @21:27:32.11 Plunge.seek :reagent @21:27:32.97 Sample loop closed. Ready to Deliver Reagents @21:27:32.98 Archive.seek :sealed @21:27:37.26 Archive.seek :evac @21:27:51.06 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 4ul (sensed 30ul) @21:27:51.08 Retry #1 of 2 @21:27:51.09 Archive.hold @21:27:51.26 Archive.reconfigure :default @21:27:51.33 Archive.home.seek :evac @21:27:51.95 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 4ul (sensed 31ul) @21:27:51.96 Retry #2 of 2 @21:27:51.98 Archive.reconfigure :default @21:27:52.05 Archive.hold @21:27:52.12 Archive.reconfigure :default @21:27:52.19 Archive.home.seek :evac @21:27:52.82 Slide::Error in PROCESSING -- IGNORED Archive Syringe (ArS) positionErr at 3ul (sensed 31ul) @21:27:52.84 Archive.reconfigure :default @21:27:52.90 Archive.hold @21:27:52.98 Archive.reconfigure :default @21:27:53.04 Archive.home.jog 0 @21:27:53.16 delay 1:00 @21:28:53.19 Archive.seek :clear @21:29:10.64 RNAlater soak for 10:00 @21:39:10.66 Archive.seek :tVent @21:39:13.24 delay 1 second @21:39:14.26 Archive.seek :evac @21:39:29.79 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 2ul (sensed 29ul) @21:39:29.80 Retry #1 of 2 @21:39:29.82 Archive.hold @21:39:29.92 Archive.reconfigure :default @21:39:29.99 Archive.home.seek :evac @21:39:30.64 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 3ul (sensed 29ul) @21:39:30.65 Retry #2 of 2 @21:39:30.67 Archive.reconfigure :default @21:39:30.74 Archive.hold @21:39:30.82 Archive.reconfigure :default @21:39:30.89 Archive.home.seek :evac @21:39:31.52 Slide::Error in PROCESSING -- IGNORED Archive Syringe (ArS) positionErr at 3ul (sensed 29ul) @21:39:31.54 Archive.reconfigure :default @21:39:31.60 Archive.hold @21:39:31.67 Archive.reconfigure :default @21:39:31.74 Archive.home.jog 0 @21:39:31.85 delay 10 seconds @21:39:41.87 Archive.seek :tVent @21:39:57.25 delay 1 second @21:39:58.27 Archive.seek :evac @21:40:13.91 delay 10 seconds @21:40:23.94 Sample preserved and evacuated @21:40:25.09 -> Cmd.stop @21:40:25.23 Carousel.seek 12 @21:40:25.40 Plunge.seek :locked @21:40:26.24 PinPort.seek :clear @21:40:26.37 Archive.seek :clear @21:40:26.50 Air.seek :clear @21:40:26.63 Lysis1.seek :clear @21:40:44.15 Archive.coast @21:40:44.19 Air.coast @21:40:44.24 Lysis1.coast @21:40:44.29 Lysis2.coast @21:40:44.34 Plunge.coast @21:40:44.39 SamplePump.coast @21:40:44.44 Toroid.coast @21:40:44.49 Carousel.coast @21:40:44.54 PinPort.coast @21:40:44.72 Gate.power :motor,:OFF @21:40:44.73 Gate.power :core,:OFF @21:40:44.76 Gate.power :analytic1,:OFF @21:40:44.78 Gate.power :analytic2,:OFF @21:40:44.80 Gate.power :analytic3,:OFF @21:40:44.82 Gate.power :main,:OFF @21:40:44.84 Safely stopped and ready to power off ##-- file closed 2019-07-28T04:49:33.319Z ##-- file opened 2019-07-28T04:49:33.320Z @21:49:33.27 -> showStatus @21:49:35.29 -> Cmd.loadCartridge -9 @21:49:35.42 Gate.power :main,:ON,limit to 5000mA @21:49:35.43 Gate.power :analytic1,:ON,limit to 5000mA @21:49:35.45 Gate.power :analytic2,:ON,limit to 5000mA @21:49:35.47 Gate.power :analytic3,:ON,limit to 5000mA @21:49:35.60 Powering core bus @21:49:37.88 SPR Module 1, FW Rev. B, FPGA Rev. C @21:49:38.02 Archive.configure :default @21:49:38.14 Air.configure :default @21:49:38.24 Lysis1.configure :default @21:49:38.34 Lysis2.configure :default @21:49:38.46 Plunge.configure :default @21:49:38.58 SamplePump.configure :default @21:49:38.70 ExtraPump.configure :default @21:49:38.82 Toroid.configure :default @21:49:38.94 Carousel.configure :default @21:49:39.06 Pressure.configure :default @21:49:39.16 PinPort.configure :default @21:49:39.22 All elf controllers running firmware version 4.58 @21:49:39.24 Gate.power :motor,:ON,limit to 5000mA @21:49:39.47 Archive.reconfigure :default @21:49:39.70 Archive.home.jog 0 @21:49:39.84 Toroid.reconfigure :default @21:49:39.92 Plunge.reconfigure :default @21:49:39.97 Lysis1.reconfigure :default @21:49:40.05 Air.reconfigure :default @21:49:40.09 Lysis2.reconfigure :default @21:49:40.13 PinPort.reconfigure :default @21:49:40.35 Lysis2.home.jog 0 @21:49:40.40 Plunge.home.jog 0 @21:49:40.46 Air.home.jog 0 @21:49:40.52 Toroid.home.jog 0 @21:49:40.58 PinPort.home.jog 0 @21:49:40.64 Lysis1.home.jog 0 @21:49:40.94 Archive.reconfigure :homing @21:49:41.14 Toroid.reconfigure :homing @21:49:41.16 Plunge.reconfigure :homing @21:49:41.21 PinPort.reconfigure :homing @21:49:41.26 Lysis2.reconfigure :homing @21:49:41.30 Air.reconfigure :homing @21:49:41.48 Air.jog 1409 @21:49:41.54 Archive.jog 1364 @21:49:41.59 Plunge.jog 1556 @21:49:41.65 Lysis2.jog 156 @21:49:41.71 PinPort.jog -106 @21:49:41.77 Toroid.jog 632 @21:49:41.86 Lysis1.reconfigure :homing @21:49:41.94 PinPort.setRawPosition! 0 @21:49:41.98 PinPort.seek :home @21:49:42.05 Lysis1.jog 1405 @21:49:42.14 PinPort.reconfigure :default @21:49:42.16 Lysis2.setRawPosition! 50 @21:49:42.23 Lysis2.seek :home @21:49:42.53 Lysis2.reconfigure :default @21:49:42.64 Air.setRawPosition! 50 @21:49:42.69 Air.seek :home @21:49:42.77 Archive.setRawPosition! 50 @21:49:42.83 Archive.seek :home @21:49:42.91 Plunge.setRawPosition! 50 @21:49:42.96 Plunge.seek :home @21:49:43.05 Air.reconfigure :default @21:49:43.13 Archive.reconfigure :default @21:49:43.25 Lysis1.setRawPosition! 50 @21:49:43.29 Lysis1.seek :home @21:49:43.38 Plunge.reconfigure :default @21:49:43.58 Lysis1.reconfigure :default @21:49:43.92 Toroid.setRawPosition! 0 @21:49:43.96 Toroid.seek :home @21:49:44.05 Toroid.reconfigure :default @21:49:44.18 Toroid.seek :clear @21:49:45.80 Carousel.reconfigure :default @21:49:45.87 Carousel.home.jog 0 @21:49:46.36 Carousel.seek 12 @21:49:46.52 Selecting Cartridge 13 @21:49:46.77 Carousel.seek 13 @21:49:47.26 Lysis1.seek :locked @21:49:51.39 Plunge.seek :locked @21:49:51.51 Air.seek :locked @21:49:55.65 Powering cartridge bus @21:49:57.77 Gate.setIR 1750,5000,0,6 @21:49:57.90 Gate.setIR 0,5000,0,6 @21:49:57.95 Loaded Lyse-n-Go Cartridge @21:49:57.97 PuckHeater.configure HeatConfig @21:49:58.02 Gate.power :heat,:ON,limit to 5000mA @21:50:14.02 -> Cmd.startFiltering @21:50:14.25 Duration of filtering limited to 2:05:00 @21:50:14.51 Priming sample loop w/100ml, bypass w/10ml @21:50:14.52 SamplePump.setPosition! 0ml @21:50:14.56 Bypass.open @21:50:16.03 Intake.open @21:50:16.81 Sea 2.5m deep (18.4psia) @21:50:16.83 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:50:19.67 Vacuum tare is 52% of full scale @21:50:19.80 Pressure.reconfigure :default @21:50:19.86 Pressure.hold @21:50:20.39 SamplePump.reconfigure :prime @21:50:20.47 SamplePump.seek 10ml,1:10 @21:50:29.43 Pressure.coast @21:50:29.49 SamplePump.setPosition! 0ml @21:50:29.54 Exhaust.open @21:50:30.94 Bypass.close @21:50:32.46 Pressure.reconfigure :default @21:50:32.52 Pressure.hold @21:50:32.63 Sea 2.3m deep (18.1psia) @21:50:32.77 SamplePump.reconfigure :prime @21:50:32.84 SamplePump.seek 100ml,7:10 @21:52:28.77 Pressure.coast @21:52:28.83 Bypass.open @21:52:29.54 Intake.close @21:52:30.37 Toroid.reconfigure :hold @21:52:30.44 Toroid.seek :crack @21:52:31.23 Toroid.reconfigure :default @21:52:31.29 Toroid.seek :clear @21:52:32.85 Toroid.reconfigure :hold @21:52:32.92 Toroid.seek :cartridge @21:52:34.73 Toroid.reconfigure :default @21:52:34.79 Toroid.seek :clear @21:52:36.77 Pressurizing cartridge to 14psi above 18.3psia @21:52:36.78 SamplePump.setPosition! 0ml @21:52:36.82 Intake.open @21:52:37.56 Bypass.close @21:52:38.30 Exhaust.close @21:52:39.11 Pressure.reconfigure :default @21:52:39.16 Pressure.hold @21:52:39.33 SamplePump.reconfigure :charge @21:52:39.40 SamplePump.seek 2.3ml,41 seconds @21:52:47.68 Pressure.coast @21:52:47.73 Intake.close @21:52:48.52 Pressurized cartridge with 0.320ml to 14.2psi @21:52:48.53 Isolated @30.2psia (11.9psi) @21:52:48.55 Checking for cartridge leaks from 30.2psia @21:52:48.58 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @21:52:53.69 Sampling 1000.0ml @21:52:53.70 SamplePump.setPosition! 0ml @21:52:53.75 Exhaust.open @21:52:54.48 Intake.open @21:52:55.33 Pressure.reconfigure :default @21:52:55.39 Pressure.hold @21:52:55.51 Sea 2.5m deep (18.4psia) @21:52:55.64 SamplePump.reconfigure :sample @21:52:55.71 SamplePump.seek 1000ml,1:07:09 @21:52:58.37 Cartridge 13 sampling 1/1000ml at 0.36ml/s with 12.2psi vacuum @21:53:58.37 Cartridge 13 sampling 21/1000ml at 0.33ml/s with 13.1psi vacuum @21:54:58.37 Cartridge 13 sampling 41/1000ml at 0.33ml/s with 12.2psi vacuum @21:55:58.37 Cartridge 13 sampling 61/1000ml at 0.34ml/s with 13.2psi vacuum @21:56:58.37 Cartridge 13 sampling 81/1000ml at 0.34ml/s with 14.4psi vacuum @21:57:58.37 Cartridge 13 sampling 100/1000ml at 0.32ml/s with 14.1psi vacuum @21:58:58.37 Cartridge 13 sampling 119/1000ml at 0.32ml/s with 11.8psi vacuum 1.9m deep (17.5psia) @21:59:58.37 Cartridge 13 sampling 138/1000ml at 0.30ml/s with 14.5psi vacuum @22:00:58.37 Cartridge 13 sampling 156/1000ml at 0.31ml/s with 11.5psi vacuum 1.9m deep (17.6psia) @22:01:58.37 Cartridge 13 sampling 174/1000ml at 0.30ml/s with 12.0psi vacuum @22:02:58.37 Cartridge 13 sampling 191/1000ml at 0.29ml/s with 14.9psi vacuum @22:03:58.37 Cartridge 13 sampling 209/1000ml at 0.27ml/s with 13.3psi vacuum @22:04:58.37 Cartridge 13 sampling 226/1000ml at 0.27ml/s with 13.1psi vacuum @22:05:58.37 Cartridge 13 sampling 242/1000ml at 0.28ml/s with 12.7psi vacuum @22:06:58.37 Cartridge 13 sampling 259/1000ml at 0.29ml/s with 13.2psi vacuum @22:07:58.37 Cartridge 13 sampling 275/1000ml at 0.26ml/s with 13.3psi vacuum @22:08:58.37 Cartridge 13 sampling 291/1000ml at 0.25ml/s with 10.9psi vacuum 2.1m deep (17.9psia) @22:09:58.37 Cartridge 13 sampling 306/1000ml at 0.25ml/s with 15.5psi vacuum @22:10:58.37 Cartridge 13 sampling 321/1000ml at 0.25ml/s with 15.2psi vacuum @22:11:58.37 Cartridge 13 sampling 337/1000ml at 0.25ml/s with 12.7psi vacuum 1.7m deep (17.3psia) @22:12:58.37 Cartridge 13 sampling 352/1000ml at 0.25ml/s with 13.6psi vacuum @22:12:59.35 Flow averaged 0.29ml/s over 20:03 @22:12:59.38 Waiting up to 7 seconds for vacuum to reduce from 14.6 to 10.0psi @22:13:00.07 Relieved excess vacuum in 1.6 seconds @22:13:00.10 SamplePump.seek 1000ml,43:42 @22:13:04.19 Flow estimate reduced to 0.23ml/s @22:13:04.20 Intake.close @22:13:04.27 Exhaust.close @22:13:04.42 Toroid.reconfigure :hold @22:13:04.49 Toroid.seek :bypass @22:13:06.11 Toroid.reconfigure :default @22:13:06.18 Toroid.seek :clear @22:13:07.80 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @22:13:07.82 Pressure.coast @22:13:08.38 Sampled 352.8ml @22:13:10.09 -> Cmd.startProcessing @22:13:11.95 SPR.command \"read_sequences\" @22:13:11.98 SPR.command \"stats_on\" @22:13:12.75 SPR.command \"start 0 1\" @22:13:14.76 Begun log /var/log/esp/spr19jul2722h.spr @22:13:15.61 Handoff.select :waste @22:13:15.84 Archive.seek :empty @22:13:23.04 Syringe tare is 21% of full scale @22:13:33.59 PinPort.seek :cartridge @22:13:38.15 Lysis1.seek 1350ul @22:13:40.64 Lysis2.seek 1700ul @22:13:43.75 Plunge.seek :reagent @22:13:44.59 Evacuating puck with filter bubble point of 30psi @22:13:44.60 PinPort.seek :cartridge @22:13:44.68 Handoff.select :waste @22:13:52.04 Air pumping 1 strokes to reach 4psi @22:13:52.05 Air.reconfigure :gulp @22:13:52.14 Air.seek :tVent @22:13:53.95 0.0psi after gulp @22:13:53.96 Air stroke #1 of 1 @22:13:53.98 Air.reconfigure :pressurize @22:13:54.05 Air.seek :pressurized @22:14:06.90 -0.0psi after pressurize @22:14:06.91 Air.reconfigure :compress @22:14:06.98 Air.seek :evac @22:14:09.65 0.0psi after compress @22:14:23.56 0.1psi after compress (stable) @22:14:23.68 at 0.1psi Opened to waste @22:14:23.69 Handoff.select :blocked @22:14:23.87 Air pumping 10 strokes to reach 30psi @22:14:23.88 Air.reconfigure :gulp @22:14:23.96 Air.seek :tVent @22:14:33.86 3.1psi after gulp @22:14:33.87 Air stroke #1 of 10 @22:14:33.88 Air.reconfigure :pressurize @22:14:33.96 Air.seek :pressurized @22:14:46.82 5.1psi after pressurize @22:14:46.85 Air.reconfigure :compress @22:14:46.93 Air.seek :evac @22:14:49.60 5.8psi after compress @22:15:46.01 26.3psi after compress (stable) @22:15:46.03 Air.reconfigure :gulp @22:15:46.12 Air.seek :tVent @22:15:56.02 28.3psi after gulp @22:15:56.03 Air stroke #2 of 10 @22:15:56.05 Air.reconfigure :pressurize @22:15:56.13 Air.seek :pressurized @22:16:08.99 27.4psi after pressurize @22:16:09.00 Air.reconfigure :compress @22:16:09.08 Air.seek :evac @22:16:11.76 26.3psi after compress @22:16:21.54 Reached 30.1 of 30.0psi after pumping 4672ul (3.00 strokes) @22:16:21.55 Handoff.select :waste @22:16:34.39 delay 1:00 @22:17:34.42 Handoff.select :blocked @22:17:45.16 Pressure too High, open to waste again (4.0psi) @22:17:45.17 Handoff.select :waste @22:17:45.29 delay 1:00 @22:18:45.31 Handoff.select :blocked @22:19:10.33 Handoff.select :waste @22:19:10.45 PSI at @0.11psi at evacEnd @22:19:10.46 Handoff.select :blocked @22:19:10.58 Air.reconfigure :gulp @22:19:10.66 Air.seek :tVent @22:19:27.73 Stable @0.2psi after Handoff blocked @22:19:27.83 Air pumping 10 strokes to reach 25psi @22:19:27.84 Air stroke #1 of 10 @22:19:27.86 Air.reconfigure :pressurize @22:19:27.94 Air.seek :pressurized @22:19:40.79 0.2psi after pressurize @22:19:40.80 Air.reconfigure :compress @22:19:40.88 Air.seek :evac @22:19:43.55 0.3psi after compress @22:20:22.19 12.8psi after compress (stable) @22:20:22.21 Air.reconfigure :gulp @22:20:22.29 Air.seek :tVent @22:20:32.18 14.0psi after gulp @22:20:32.19 Air stroke #2 of 10 @22:20:32.21 Air.reconfigure :pressurize @22:20:32.29 Air.seek :pressurized @22:20:45.14 14.2psi after pressurize @22:20:45.15 Air.reconfigure :compress @22:20:45.23 Air.seek :evac @22:20:47.90 14.3psi after compress @22:21:20.63 22.7psi after compress (stable) @22:21:20.65 Air.reconfigure :gulp @22:21:20.73 Air.seek :tVent @22:21:30.63 23.8psi after gulp @22:21:30.64 Air stroke #3 of 10 @22:21:30.66 Air.reconfigure :pressurize @22:21:30.76 Air.seek :pressurized @22:21:43.69 23.9psi after pressurize @22:21:43.71 Air.reconfigure :compress @22:21:43.78 Air.seek :evac @22:21:46.47 23.9psi after compress @22:21:50.42 Reached 25.3 of 25.2psi after pumping 6230ul (4.00 strokes) @22:21:50.43 Handoff.select :waste @22:21:59.46 delay 1:00 @22:22:59.48 Handoff.select :blocked @22:23:24.51 Handoff.select :waste @22:23:24.65 PSI at @2.10psi at evacEnd @22:23:24.66 Air.reconfigure :default @22:23:24.75 Air.seek :fVent @22:23:26.19 delay 5 seconds @22:23:31.21 Seawater evacuated, Presure above/below filter released. Lysis begins @22:23:31.28 Lysis1.reconfigure :slow @22:23:31.34 Lysis1.seek 1050ul @22:24:01.42 delay 5 seconds @22:24:06.44 Handoff.select :blocked @22:24:06.60 Lysis1.seek 450ul @22:25:06.63 delay 3 seconds @22:25:09.70 Air.reconfigure to mix into 1.37806 parts Lysis1 @22:25:10.22 Air.seek :evac @22:25:10.27 Lysis1.seek 300ul @22:25:25.39 Air.reconfigure :default @22:25:25.45 Air.seek :full @22:25:39.68 Lysis1.seek :empty @22:26:09.82 Lysis Delivered. Syringe pressure @43.9psi @22:26:09.87 PuckHeater.seek 85.7C @22:27:12.00 PuckHeater.hold 15:00,85.7C @22:42:09.05 Lysis complete. Syringe pressure @53.3psi @22:42:09.07 Handoff.select :blocked @22:42:09.17 Lysis1.reconfigure :halfSpeed @22:42:09.23 Lysis1.seek 200ul @22:42:13.01 delay 1 second @22:42:14.03 Lysis1.seek :empty @22:42:17.86 Initial AirS pressure @54.6psi @22:42:17.92 Lysis1.reconfigure :extract @22:42:18.02 Lysis1.seek 875ul,10:00 @22:42:19.17 Air pumping 10 strokes to reach 21psi @22:42:19.20 Air stroke #1 of 10 @22:42:19.23 Air.reconfigure :pressurize @22:42:19.31 Air.seek :pressurized @22:42:32.16 15.6psi after pressurize @22:42:32.17 Air.reconfigure :compress @22:42:32.25 Air.seek :evac @22:42:35.25 15.5psi after compress @22:42:49.15 15.8psi after compress (stable) @22:42:49.17 Air.reconfigure :gulp @22:42:49.24 Air.seek :tVent @22:42:59.14 15.9psi after gulp @22:42:59.15 Air stroke #2 of 10 @22:42:59.17 Air.reconfigure :pressurize @22:42:59.25 Air.seek :pressurized @22:43:12.09 15.8psi after pressurize @22:43:12.10 Air.reconfigure :compress @22:43:12.19 Air.seek :evac @22:43:14.85 16.2psi after compress @22:43:28.77 15.8psi after compress (stable) @22:43:28.79 Air.reconfigure :gulp @22:43:28.86 Air.seek :tVent @22:43:38.76 15.3psi after gulp @22:43:38.77 Air stroke #3 of 10 @22:43:38.79 Air.reconfigure :pressurize @22:43:38.86 Air.seek :pressurized @22:43:51.71 16.1psi after pressurize @22:43:51.72 Air.reconfigure :compress @22:43:51.80 Air.seek :evac @22:43:54.46 15.9psi after compress @22:44:02.54 Air.reconfigure :default @22:44:02.60 Air.hold @22:44:02.77 Lysis1 at 875ul pressure @15.9psi @22:44:02.78 Lysis1.reconfigure :default @22:44:02.86 Lysis1.seek 900ul @22:44:03.63 Lysis1 at 900ul pressure(finPSI) @10.1psi @22:44:03.64 delay 1 second @22:44:04.75 Lysis1 at 900ul pressure @11.8psi @22:44:04.77 Air.reconfigure :halfSpeed @22:44:04.85 Air.seek :fVent @22:44:07.10 Puck Top Vented. Syringe pressure @2.3psi @22:44:07.12 Handoff.select :waste @22:44:07.24 delay 5 seconds @22:44:12.26 Lysate Recovered in LyS1 @22:44:12.28 Lysis2.reconfigure :DAmix @22:44:12.39 Lysis1.reconfigure :DAmix @22:44:12.87 Lysis2.seek 1450ul @22:44:12.95 Lysis1.seek 650ul @22:44:48.00 delay 5 seconds @22:44:53.02 Handoff.select SPR @22:44:53.13 SPR.command \"start 2 14\" @22:44:54.45 delay 10 seconds @22:45:04.47 SPR.command \"start 1 0\" @22:45:07.24 Waiting up to 4:00 for SPR to enter state 1 @22:47:39.86 SPR.command \"hold_on\" @22:47:41.30 SPR Dilution 1 of 1 -- Running Experiment 1 @22:47:41.72 Lysis2.seek 1150ul @22:47:41.76 Lysis1.seek 350ul @22:48:23.85 volL1 = 300.0 and volL2 = 300.0 @22:48:23.86 delay 15 seconds @22:48:38.89 SPR.command \"hold_off\" @22:48:41.94 Waiting up to 25:00 for SPR to enter state 7 @23:08:55.78 SPR Analysis has Completed @23:08:55.80 Handoff.select :waste @23:08:56.72 Ended log /var/log/esp/spr19jul2722h.spr @23:08:56.73 SPR.command \"stats_off\" @23:08:58.02 -> Cmd.stop @23:08:58.11 Handoff.select :close @23:08:58.24 Gate.power :heat,:OFF @23:08:58.26 Gate.power :cartridge,:OFF @23:08:58.33 Carousel.seek 13 @23:08:58.51 Plunge.seek :locked @23:08:59.36 PinPort.seek :clear @23:09:03.98 Archive.seek :clear @23:09:04.11 Air.reconfigure :default @23:09:04.25 Lysis1.reconfigure :default @23:09:04.35 Air.seek :clear @23:09:04.41 Lysis1.seek :clear @23:09:04.50 Lysis2.reconfigure :default @23:09:04.57 Lysis2.seek :clear @23:09:21.74 Archive.coast @23:09:21.79 Air.coast @23:09:21.84 Lysis1.coast @23:09:21.89 Lysis2.coast @23:09:21.94 Plunge.coast @23:09:21.99 SamplePump.coast @23:09:22.38 Toroid.coast @23:09:22.43 Carousel.coast @23:09:22.48 PinPort.coast @23:09:22.67 Gate.power :motor,:OFF @23:09:22.68 Gate.power :core,:OFF @23:09:22.71 Gate.power :analytic1,:OFF @23:09:22.73 Gate.power :analytic2,:OFF @23:09:22.75 Gate.power :analytic3,:OFF @23:09:22.77 Gate.power :main,:OFF @23:09:22.79 Safely stopped and ready to power off ##-- file closed 2019-07-28T06:18:17.347Z ##-- file opened 2019-07-28T06:18:17.349Z @23:18:17.78 -> showStatus @23:18:19.80 -> Cmd.loadCartridge -9 @23:18:19.93 Gate.power :main,:ON,limit to 5000mA @23:18:19.94 Gate.power :analytic1,:ON,limit to 5000mA @23:18:19.97 Gate.power :analytic2,:ON,limit to 5000mA @23:18:19.99 Gate.power :analytic3,:ON,limit to 5000mA @23:18:20.12 Powering core bus @23:18:22.40 SPR Module 1, FW Rev. B, FPGA Rev. C @23:18:22.54 Archive.configure :default @23:18:22.66 Air.configure :default @23:18:22.76 Lysis1.configure :default @23:18:22.86 Lysis2.configure :default @23:18:22.98 Plunge.configure :default @23:18:23.13 SamplePump.configure :default @23:18:23.25 ExtraPump.configure :default @23:18:23.37 Toroid.configure :default @23:18:23.49 Carousel.configure :default @23:18:23.60 Pressure.configure :default @23:18:23.70 PinPort.configure :default @23:18:23.76 All elf controllers running firmware version 4.58 @23:18:23.79 Gate.power :motor,:ON,limit to 5000mA @23:18:23.94 Archive.reconfigure :default @23:18:24.22 Archive.home.jog 0 @23:18:24.35 Toroid.reconfigure :default @23:18:24.43 Plunge.reconfigure :default @23:18:24.47 Lysis1.reconfigure :default @23:18:24.51 PinPort.reconfigure :default @23:18:24.55 Air.reconfigure :default @23:18:24.64 Lysis2.reconfigure :default @23:18:24.82 Lysis2.home.jog 0 @23:18:24.87 Plunge.home.jog 0 @23:18:24.93 Air.home.jog 0 @23:18:24.98 Lysis1.home.jog 0 @23:18:25.04 PinPort.home.jog 0 @23:18:25.10 Toroid.home.jog 0 @23:18:25.44 Archive.reconfigure :homing @23:18:25.68 Toroid.reconfigure :homing @23:18:25.72 Plunge.reconfigure :homing @23:18:25.75 Air.reconfigure :homing @23:18:25.79 PinPort.reconfigure :homing @23:18:25.82 Lysis2.reconfigure :homing @23:18:25.86 Lysis1.reconfigure :homing @23:18:26.06 Air.jog 152 @23:18:26.12 Lysis1.jog 1407 @23:18:26.17 Toroid.jog 634 @23:18:26.23 Plunge.jog 1564 @23:18:26.28 PinPort.jog -105 @23:18:26.34 Archive.jog 1408 @23:18:26.40 Lysis2.jog 179 @23:18:26.48 Air.setRawPosition! 50 @23:18:26.54 Air.seek :home @23:18:26.62 PinPort.setRawPosition! 0 @23:18:26.66 PinPort.seek :home @23:18:26.75 PinPort.reconfigure :default @23:18:26.85 Air.reconfigure :default @23:18:26.91 Lysis2.setRawPosition! 50 @23:18:26.96 Lysis2.seek :home @23:18:27.26 Lysis2.reconfigure :default @23:18:27.33 Lysis1.setRawPosition! 50 @23:18:27.38 Lysis1.seek :home @23:18:27.49 Plunge.setRawPosition! 50 @23:18:27.53 Plunge.seek :home @23:18:27.61 Archive.setRawPosition! 50 @23:18:27.65 Archive.seek :home @23:18:27.75 Lysis1.reconfigure :default @23:18:27.82 Plunge.reconfigure :default @23:18:27.95 Archive.reconfigure :default @23:18:28.33 Toroid.setRawPosition! 0 @23:18:28.37 Toroid.seek :home @23:18:28.46 Toroid.reconfigure :default @23:18:28.58 Toroid.seek :clear @23:18:30.20 Carousel.reconfigure :default @23:18:30.27 Carousel.home.jog 0 @23:18:30.77 Carousel.seek 13 @23:18:30.91 Selecting Cartridge 11 @23:18:31.15 Carousel.seek 11 @23:18:31.83 Lysis1.seek :locked @23:18:35.96 Plunge.seek :locked @23:18:36.08 Air.seek :locked @23:18:40.21 Powering cartridge bus @23:18:42.33 Gate.setIR 1750,5000,0,6 @23:18:42.46 Gate.setIR 0,5000,0,6 @23:18:42.51 Loaded Lyse-n-Go Cartridge @23:18:42.53 PuckHeater.configure HeatConfig @23:18:42.58 Gate.power :heat,:ON,limit to 5000mA @23:18:58.65 -> Cmd.startFiltering @23:18:58.87 Duration of filtering limited to 2:05:00 @23:18:59.13 Priming sample loop w/100ml, bypass w/10ml @23:18:59.14 SamplePump.setPosition! 0ml @23:18:59.18 Bypass.open @23:19:00.66 Intake.open @23:19:01.46 Sea 2.6m deep (18.5psia) @23:19:01.48 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @23:19:04.31 Vacuum tare is 52% of full scale @23:19:04.44 Pressure.reconfigure :default @23:19:04.86 Pressure.hold @23:19:05.03 SamplePump.reconfigure :prime @23:19:05.10 SamplePump.seek 10ml,1:10 @23:19:14.06 Pressure.coast @23:19:14.11 SamplePump.setPosition! 0ml @23:19:14.16 Exhaust.open @23:19:15.59 Bypass.close @23:19:17.08 Pressure.reconfigure :default @23:19:17.14 Pressure.hold @23:19:17.26 Sea 2.5m deep (18.4psia) @23:19:17.39 SamplePump.reconfigure :prime @23:19:17.46 SamplePump.seek 100ml,7:10 @23:21:13.19 Pressure.coast @23:21:13.25 Bypass.open @23:21:13.93 Intake.close @23:21:14.78 Toroid.reconfigure :hold @23:21:14.84 Toroid.seek :crack @23:21:15.64 Toroid.reconfigure :default @23:21:15.70 Toroid.seek :clear @23:21:17.27 Toroid.reconfigure :hold @23:21:17.33 Toroid.seek :cartridge @23:21:19.14 Toroid.reconfigure :default @23:21:19.20 Toroid.seek :clear @23:21:21.17 Pressurizing cartridge to 14psi above 18.5psia @23:21:21.18 SamplePump.setPosition! 0ml @23:21:21.22 Intake.open @23:21:22.00 Bypass.close @23:21:22.69 Exhaust.close @23:21:23.48 Pressure.reconfigure :default @23:21:23.54 Pressure.hold @23:21:23.71 SamplePump.reconfigure :charge @23:21:23.78 SamplePump.seek 2.3ml,41 seconds @23:21:33.19 Pressure.coast @23:21:33.24 Intake.close @23:21:34.06 Pressurized cartridge with 0.413ml to 14.1psi @23:21:34.07 Isolated @30.2psia (11.7psi) @23:21:34.08 Checking for cartridge leaks from 30.2psia @23:21:34.11 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @23:21:39.23 Sampling 1000.0ml @23:21:39.25 SamplePump.setPosition! 0ml @23:21:39.30 Exhaust.open @23:21:39.99 Intake.open @23:21:40.33 Pressure.reconfigure :default @23:21:40.38 Pressure.hold @23:21:40.50 Sea 4.1m deep (20.8psia) @23:21:40.63 SamplePump.reconfigure :sample @23:21:40.70 SamplePump.seek 1000ml,1:07:09 @23:21:43.36 Cartridge 11 sampling 1/1000ml at 0.43ml/s with 11.6psi vacuum 1.8m deep (17.4psia) @23:22:43.37 Cartridge 11 sampling 21/1000ml at 0.36ml/s with 14.8psi vacuum @23:23:43.37 Cartridge 11 sampling 41/1000ml at 0.35ml/s with 13.5psi vacuum @23:24:43.37 Cartridge 11 sampling 61/1000ml at 0.34ml/s with 13.1psi vacuum @23:25:43.37 Cartridge 11 sampling 81/1000ml at 0.34ml/s with 12.1psi vacuum 2.1m deep (17.8psia) @23:26:43.37 Cartridge 11 sampling 100/1000ml at 0.30ml/s with 11.4psi vacuum @23:27:43.37 Cartridge 11 sampling 118/1000ml at 0.31ml/s with 9.6psi vacuum 2.1m deep (17.8psia) @23:28:43.37 Cartridge 11 sampling 137/1000ml at 0.33ml/s with 13.1psi vacuum 1.7m deep (17.2psia) @23:29:43.37 Cartridge 11 sampling 155/1000ml at 0.29ml/s with 10.6psi vacuum 2.3m deep (18.1psia) @23:30:43.37 Cartridge 11 sampling 174/1000ml at 0.28ml/s with 12.2psi vacuum @23:31:43.37 Cartridge 11 sampling 191/1000ml at 0.27ml/s with 14.0psi vacuum @23:32:43.37 Cartridge 11 sampling 209/1000ml at 0.29ml/s with 11.9psi vacuum @23:33:43.43 Cartridge 11 sampling 226/1000ml at 0.27ml/s with 15.1psi vacuum @23:34:43.37 Cartridge 11 sampling 244/1000ml at 0.30ml/s with 12.0psi vacuum @23:35:43.37 Cartridge 11 sampling 260/1000ml at 0.29ml/s with 10.6psi vacuum @23:36:43.37 Cartridge 11 sampling 277/1000ml at 0.29ml/s with 14.0psi vacuum @23:37:43.37 Cartridge 11 sampling 294/1000ml at 0.28ml/s with 13.1psi vacuum @23:38:43.37 Cartridge 11 sampling 310/1000ml at 0.30ml/s with 13.6psi vacuum @23:39:43.37 Cartridge 11 sampling 326/1000ml at 0.26ml/s with 12.7psi vacuum @23:40:43.37 Cartridge 11 sampling 342/1000ml at 0.26ml/s with 11.8psi vacuum 1.9m deep (17.5psia) @23:41:43.37 Cartridge 11 sampling 358/1000ml at 0.26ml/s with 14.9psi vacuum @23:42:43.37 Cartridge 11 sampling 373/1000ml at 0.25ml/s with 12.8psi vacuum @23:43:43.37 Cartridge 11 sampling 389/1000ml at 0.27ml/s with 13.7psi vacuum @23:44:43.37 Cartridge 11 sampling 404/1000ml at 0.25ml/s with 11.5psi vacuum @23:45:43.37 Cartridge 11 sampling 419/1000ml at 0.25ml/s with 15.3psi vacuum @23:46:43.37 Cartridge 11 sampling 434/1000ml at 0.25ml/s with 12.1psi vacuum @23:47:43.37 Cartridge 11 sampling 450/1000ml at 0.25ml/s with 17.8psi vacuum @23:48:15.66 Flow averaged 0.29ml/s over 26:33 @23:48:15.69 Waiting up to 7 seconds for vacuum to reduce from 14.7 to 10.0psi @23:48:16.39 Relieved excess vacuum in 3.5 seconds @23:48:16.41 SamplePump.seek 1000ml,36:39 @23:48:35.78 Flow averaged 0.37ml/s over 14 seconds @23:48:35.81 Waiting up to 7 seconds for vacuum to reduce from 14.5 to 10.0psi @23:48:36.89 Relieved excess vacuum in 7.4 seconds @23:48:36.92 SamplePump.seek 1000ml,36:20 @23:48:43.36 Cartridge 11 sampling 464/1000ml at 0.25ml/s with 15.1psi vacuum @23:49:08.92 Flow averaged 0.30ml/s over 27 seconds @23:49:08.95 Waiting up to 6 seconds for vacuum to reduce from 14.3 to 10.0psi @23:49:09.65 Relieved excess vacuum in 6.2 seconds @23:49:09.67 SamplePump.seek 1000ml,35:48 @23:49:43.36 Cartridge 11 sampling 479/1000ml at 0.25ml/s with 15.0psi vacuum @23:49:45.44 Flow averaged 0.27ml/s over 34 seconds @23:49:45.47 Waiting up to 6 seconds for vacuum to reduce from 14.4 to 10.0psi @23:49:46.17 Relieved excess vacuum in 2.7 seconds @23:49:46.19 SamplePump.seek 1000ml,35:12 @23:49:56.67 Flow estimate reduced to 0.21ml/s @23:49:56.69 Intake.close @23:49:56.74 Exhaust.close @23:49:56.92 Toroid.reconfigure :hold @23:49:56.99 Toroid.seek :bypass @23:49:58.61 Toroid.reconfigure :default @23:49:58.68 Toroid.seek :clear @23:50:00.30 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @23:50:00.32 Pressure.coast @23:50:00.50 Sampled 481.9ml @23:50:02.73 -> Cmd.startProcessing @23:50:04.58 SPR.command \"read_sequences\" @23:50:05.31 SPR.command \"stats_on\" @23:50:05.35 SPR.command \"start 0 1\" @23:50:07.36 Begun log /var/log/esp/spr19jul2723h.spr @23:50:08.17 Handoff.select :waste @23:50:08.35 Archive.seek :empty @23:50:15.52 Syringe tare is 20% of full scale @23:50:26.08 PinPort.seek :cartridge @23:50:30.64 Lysis1.seek 1350ul @23:50:33.11 Lysis2.seek 1700ul @23:50:36.21 Plunge.seek :reagent @23:50:37.05 Evacuating puck with filter bubble point of 30psi @23:50:37.06 PinPort.seek :cartridge @23:50:37.14 Handoff.select :waste @23:50:44.46 Air pumping 1 strokes to reach 4psi @23:50:44.48 Air.reconfigure :gulp @23:50:44.57 Air.seek :tVent @23:50:46.37 -0.0psi after gulp @23:50:46.38 Air stroke #1 of 1 @23:50:46.40 Air.reconfigure :pressurize @23:50:46.49 Air.seek :pressurized @23:50:59.33 0.1psi after pressurize @23:50:59.34 Air.reconfigure :compress @23:50:59.42 Air.seek :evac @23:51:02.08 0.5psi after compress @23:51:15.96 0.3psi after compress (stable) @23:51:16.08 at 0.2psi Opened to waste @23:51:16.09 Handoff.select :blocked @23:51:16.27 Air pumping 10 strokes to reach 30psi @23:51:16.29 Air.reconfigure :gulp @23:51:16.36 Air.seek :tVent @23:51:26.25 9.9psi after gulp @23:51:26.26 Air stroke #1 of 10 @23:51:26.28 Air.reconfigure :pressurize @23:51:26.36 Air.seek :pressurized @23:51:39.19 12.8psi after pressurize @23:51:39.20 Air.reconfigure :compress @23:51:39.65 Air.seek :evac @23:51:42.33 16.6psi after compress @23:51:48.54 Reached 30.1 of 30.0psi after pumping 3115ul (2.00 strokes) @23:51:48.55 Handoff.select :waste @23:51:57.02 delay 1:00 @23:52:57.04 Handoff.select :blocked @23:52:59.44 Pressure too High, open to waste again (4.5psi) @23:52:59.45 Handoff.select :waste @23:52:59.57 delay 1:00 @23:53:59.59 Handoff.select :blocked @23:54:24.59 Handoff.select :waste @23:54:24.71 PSI at @2.32psi at evacEnd @23:54:24.72 Handoff.select :blocked @23:54:24.84 Air.reconfigure :gulp @23:54:24.92 Air.seek :tVent @23:54:42.00 Stable @2.0psi after Handoff blocked @23:54:42.09 Air pumping 10 strokes to reach 27psi @23:54:42.10 Air stroke #1 of 10 @23:54:42.11 Air.reconfigure :pressurize @23:54:42.19 Air.seek :pressurized @23:54:55.03 2.5psi after pressurize @23:54:55.04 Air.reconfigure :compress @23:54:55.12 Air.seek :evac @23:54:58.14 4.0psi after compress @23:55:28.61 14.3psi after compress (stable) @23:55:28.63 Air.reconfigure :gulp @23:55:28.70 Air.seek :tVent @23:55:38.60 14.8psi after gulp @23:55:38.61 Air stroke #2 of 10 @23:55:38.62 Air.reconfigure :pressurize @23:55:38.73 Air.seek :pressurized @23:55:51.56 15.0psi after pressurize @23:55:51.57 Air.reconfigure :compress @23:55:51.65 Air.seek :evac @23:55:54.31 16.0psi after compress @23:56:18.16 25.0psi after compress (stable) @23:56:18.18 Air.reconfigure :gulp @23:56:18.25 Air.seek :tVent @23:56:28.15 25.6psi after gulp @23:56:28.16 Air stroke #3 of 10 @23:56:28.18 Air.reconfigure :pressurize @23:56:28.26 Air.seek :pressurized @23:56:41.10 25.6psi after pressurize @23:56:41.12 Air.reconfigure :compress @23:56:41.20 Air.seek :evac @23:56:44.28 26.7psi after compress @23:56:44.53 Reached 27.2 of 27.0psi after pumping 6230ul (4.00 strokes) @23:56:44.55 Handoff.select :waste @23:56:52.53 delay 1:00 @23:57:52.55 Handoff.select :blocked @23:58:17.57 Handoff.select :waste @23:58:17.71 PSI at @1.05psi at evacEnd @23:58:17.72 Air.reconfigure :default @23:58:17.81 Air.seek :fVent @23:58:19.24 delay 5 seconds @23:58:24.26 Seawater evacuated, Presure above/below filter released. Lysis begins @23:58:24.33 Lysis1.reconfigure :slow @23:58:24.39 Lysis1.seek 1050ul @23:58:54.43 delay 5 seconds @23:58:59.45 Handoff.select :blocked @23:58:59.61 Lysis1.seek 450ul @23:59:59.58 delay 3 seconds @00:00:02.66PDT28-Jul-19 Air.reconfigure to mix into 1.37806 parts Lysis1 @00:00:03.16 Air.seek :evac @00:00:03.20 Lysis1.seek 300ul @00:00:18.32 Air.reconfigure :default @00:00:18.38 Air.seek :full @00:00:32.60 Lysis1.seek :empty @00:01:02.70 Lysis Delivered. Syringe pressure @24.3psi @00:01:02.75 PuckHeater.seek 85.7C @00:02:11.17 PuckHeater.hold 15:00,85.7C @00:17:09.60 Lysis complete. Syringe pressure @26.6psi @00:17:09.61 Handoff.select :blocked @00:17:09.71 Lysis1.reconfigure :halfSpeed @00:17:09.80 Lysis1.seek 200ul @00:17:13.56 delay 1 second @00:17:14.59 Lysis1.seek :empty @00:17:18.41 Initial AirS pressure @26.9psi @00:17:18.47 Lysis1.reconfigure :extract @00:17:18.56 Lysis1.seek 875ul,10:00 @00:17:19.71 Air pumping 10 strokes to reach 21psi @00:17:19.72 Air stroke #1 of 10 @00:17:19.74 Air.reconfigure :pressurize @00:17:19.82 Air.seek :pressurized @00:17:32.65 13.4psi after pressurize @00:17:32.66 Air.reconfigure :compress @00:17:32.74 Air.seek :evac @00:17:35.41 18.5psi after compress @00:17:42.15 Reached 20.4 of 21.0psi after pumping 3115ul (2.00 strokes) @00:17:42.84 Air pumping 8.0 strokes to reach 21psi @00:17:42.88 Air.reconfigure :gulp @00:17:42.95 Air.seek :tVent @00:17:52.85 15.8psi after gulp @00:17:52.86 Air stroke #1 of 8.0 @00:17:52.87 Air.reconfigure :pressurize @00:17:52.95 Air.seek :pressurized @00:18:05.78 15.8psi after pressurize @00:18:05.79 Air.reconfigure :compress @00:18:05.87 Air.seek :evac @00:18:08.53 15.5psi after compress @00:18:22.40 15.8psi after compress (stable) @00:18:22.42 Air.reconfigure :gulp @00:18:22.49 Air.seek :tVent @00:18:30.62 Air.reconfigure :default @00:18:30.68 Air.hold @00:18:31.47 Lysis1 at 875ul pressure @16.3psi @00:18:31.48 Lysis1.reconfigure :default @00:18:31.56 Lysis1.seek 900ul @00:18:32.33 Lysis1 at 900ul pressure(finPSI) @10.1psi @00:18:32.34 delay 1 second @00:18:33.45 Lysis1 at 900ul pressure @10.8psi @00:18:33.47 Air.reconfigure :halfSpeed @00:18:33.54 Air.seek :fVent @00:19:00.09 Puck Top Vented. Syringe pressure @2.7psi @00:19:00.10 Handoff.select :waste @00:19:00.22 delay 5 seconds @00:19:05.24 Lysate Recovered in LyS1 @00:19:05.25 Lysis1.reconfigure :DAmix @00:19:05.36 Lysis2.reconfigure :DAmix @00:19:05.81 Lysis1.seek 650ul @00:19:05.84 Lysis2.seek 1450ul @00:19:40.90 delay 5 seconds @00:19:45.93 Handoff.select SPR @00:19:46.04 SPR.command \"start 2 14\" @00:19:47.37 delay 10 seconds @00:19:57.39 SPR.command \"start 1 0\" @00:20:00.63 Waiting up to 4:00 for SPR to enter state 1 @00:22:33.25 SPR.command \"hold_on\" @00:22:34.68 SPR Dilution 1 of 1 -- Running Experiment 1 @00:22:35.09 Lysis1.seek 350ul @00:22:35.14 Lysis2.seek 1150ul @00:23:17.18 volL1 = 300.0 and volL2 = 300.0 @00:23:17.19 delay 15 seconds @00:23:32.22 SPR.command \"hold_off\" @00:23:35.33 Waiting up to 25:00 for SPR to enter state 7 @00:43:49.17 SPR Analysis has Completed @00:43:49.18 Handoff.select :waste @00:43:50.11 Ended log /var/log/esp/spr19jul2723h.spr @00:43:50.12 SPR.command \"stats_off\" @00:43:51.54 -> Cmd.stop @00:43:51.64 Handoff.select :close @00:43:51.77 Gate.power :heat,:OFF @00:43:51.79 Gate.power :cartridge,:OFF @00:43:51.87 Carousel.seek 11 @00:43:52.04 Plunge.seek :locked @00:43:52.88 PinPort.seek :clear @00:43:57.49 Archive.seek :clear @00:43:57.62 Air.reconfigure :default @00:43:57.74 Lysis1.reconfigure :default @00:43:57.85 Air.seek :clear @00:43:57.91 Lysis1.seek :clear @00:43:57.99 Lysis2.reconfigure :default @00:43:58.06 Lysis2.seek :clear @00:44:15.25 Archive.coast @00:44:15.30 Air.coast @00:44:15.35 Lysis1.coast @00:44:15.40 Lysis2.coast @00:44:15.45 Plunge.coast @00:44:15.50 SamplePump.coast @00:44:15.55 Toroid.coast @00:44:15.96 Carousel.coast @00:44:16.01 PinPort.coast @00:44:16.19 Gate.power :motor,:OFF @00:44:16.21 Gate.power :core,:OFF @00:44:16.23 Gate.power :analytic1,:OFF @00:44:16.25 Gate.power :analytic2,:OFF @00:44:16.27 Gate.power :analytic3,:OFF @00:44:16.29 Gate.power :main,:OFF @00:44:16.31 Safely stopped and ready to power off ##-- file closed 2019-07-28T07:53:10.520Z ##-- file opened 2019-07-28T07:53:10.522Z @00:53:10.57 -> showStatus @00:53:12.59 -> Cmd.loadCartridge -9 @00:53:12.72 Gate.power :main,:ON,limit to 5000mA @00:53:12.73 Gate.power :analytic1,:ON,limit to 5000mA @00:53:12.75 Gate.power :analytic2,:ON,limit to 5000mA @00:53:12.77 Gate.power :analytic3,:ON,limit to 5000mA @00:53:12.90 Powering core bus @00:53:15.18 SPR Module 1, FW Rev. B, FPGA Rev. C @00:53:15.31 Archive.configure :default @00:53:15.43 Air.configure :default @00:53:15.53 Lysis1.configure :default @00:53:15.63 Lysis2.configure :default @00:53:15.75 Plunge.configure :default @00:53:15.87 SamplePump.configure :default @00:53:15.99 ExtraPump.configure :default @00:53:16.14 Toroid.configure :default @00:53:16.26 Carousel.configure :default @00:53:16.38 Pressure.configure :default @00:53:16.49 PinPort.configure :default @00:53:16.54 All elf controllers running firmware version 4.58 @00:53:16.57 Gate.power :motor,:ON,limit to 5000mA @00:53:16.73 Archive.reconfigure :default @00:53:17.03 Archive.home.jog 0 @00:53:17.19 Toroid.reconfigure :default @00:53:17.23 Plunge.reconfigure :default @00:53:17.26 Lysis1.reconfigure :default @00:53:17.32 PinPort.reconfigure :default @00:53:17.37 Lysis2.reconfigure :default @00:53:17.41 Air.reconfigure :default @00:53:17.62 Air.home.jog 0 @00:53:17.67 Toroid.home.jog 0 @00:53:17.73 Lysis2.home.jog 0 @00:53:17.79 Lysis1.home.jog 0 @00:53:17.85 PinPort.home.jog 0 @00:53:17.91 Plunge.home.jog 0 @00:53:18.17 Archive.reconfigure :homing @00:53:18.34 Archive.jog 1407 @00:53:18.52 Toroid.reconfigure :homing @00:53:18.54 Plunge.reconfigure :homing @00:53:18.57 PinPort.reconfigure :homing @00:53:18.61 Lysis2.reconfigure :homing @00:53:18.64 Lysis1.reconfigure :homing @00:53:18.69 Air.reconfigure :homing @00:53:18.92 Lysis1.jog 1405 @00:53:18.97 Air.jog 1414 @00:53:19.02 Plunge.jog 1557 @00:53:19.08 PinPort.jog -106 @00:53:19.15 Lysis2.jog 163 @00:53:19.20 Toroid.jog 633 @00:53:19.31 PinPort.setRawPosition! 0 @00:53:19.35 PinPort.seek :home @00:53:19.44 PinPort.reconfigure :default @00:53:19.53 Archive.setRawPosition! 50 @00:53:19.58 Archive.seek :home @00:53:19.65 Lysis2.setRawPosition! 50 @00:53:19.69 Lysis2.seek :home @00:53:19.86 Archive.reconfigure :default @00:53:19.97 Lysis2.reconfigure :default @00:53:20.07 Lysis1.setRawPosition! 50 @00:53:20.12 Lysis1.seek :home @00:53:20.21 Air.setRawPosition! 50 @00:53:20.27 Air.seek :home @00:53:20.35 Plunge.setRawPosition! 50 @00:53:20.40 Plunge.seek :home @00:53:20.49 Lysis1.reconfigure :default @00:53:20.57 Air.reconfigure :default @00:53:20.69 Plunge.reconfigure :default @00:53:21.35 Toroid.setRawPosition! 0 @00:53:21.39 Toroid.seek :home @00:53:21.48 Toroid.reconfigure :default @00:53:21.60 Toroid.seek :clear @00:53:23.22 Carousel.reconfigure :default @00:53:23.29 Carousel.home.jog 0 @00:53:23.79 Carousel.seek 11 @00:53:23.93 Selecting Cartridge 10 @00:53:24.17 Carousel.seek 10 @00:53:24.65 Lysis1.seek :locked @00:53:28.79 Plunge.seek :locked @00:53:28.91 Air.seek :locked @00:53:33.04 Powering cartridge bus @00:53:35.16 Gate.setIR 1750,5000,0,6 @00:53:35.29 Gate.setIR 0,5000,0,6 @00:53:35.34 Loaded Lyse-n-Go Cartridge @00:53:35.35 PuckHeater.configure HeatConfig @00:53:35.41 Gate.power :heat,:ON,limit to 5000mA @00:53:51.43 -> Cmd.startFiltering @00:53:51.65 Duration of filtering limited to 2:05:00 @00:53:51.92 Priming sample loop w/100ml, bypass w/10ml @00:53:51.93 SamplePump.setPosition! 0ml @00:53:51.97 Bypass.open @00:53:53.44 Intake.open @00:53:54.23 Sea 2.2m deep (18.0psia) @00:53:54.25 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @00:53:57.09 Vacuum tare is 52% of full scale @00:53:57.57 Pressure.reconfigure :default @00:53:57.63 Pressure.hold @00:53:57.80 SamplePump.reconfigure :prime @00:53:57.88 SamplePump.seek 10ml,1:10 @00:54:06.85 Pressure.coast @00:54:06.91 SamplePump.setPosition! 0ml @00:54:06.95 Exhaust.open @00:54:08.38 Bypass.close @00:54:09.90 Pressure.reconfigure :default @00:54:09.96 Pressure.hold @00:54:10.08 Sea 2.0m deep (17.7psia) @00:54:10.21 SamplePump.reconfigure :prime @00:54:10.28 SamplePump.seek 100ml,7:10 @00:56:05.72 Pressure.coast @00:56:05.77 Bypass.open @00:56:06.48 Intake.close @00:56:07.28 Toroid.reconfigure :hold @00:56:07.34 Toroid.seek :crack @00:56:08.14 Toroid.reconfigure :default @00:56:08.20 Toroid.seek :clear @00:56:09.76 Toroid.reconfigure :hold @00:56:09.83 Toroid.seek :cartridge @00:56:11.66 Toroid.reconfigure :default @00:56:11.73 Toroid.seek :clear @00:56:13.69 Pressurizing cartridge to 14psi above 17.9psia @00:56:13.71 SamplePump.setPosition! 0ml @00:56:13.75 Intake.open @00:56:13.90 Bypass.close @00:56:14.62 Exhaust.close @00:56:15.44 Pressure.reconfigure :default @00:56:15.49 Pressure.hold @00:56:15.66 SamplePump.reconfigure :charge @00:56:15.74 SamplePump.seek 2.3ml,41 seconds @00:56:24.66 Pressure.coast @00:56:24.71 Intake.close @00:56:25.54 Pressurized cartridge with 0.372ml to 14.4psi @00:56:25.55 Isolated @29.9psia (12.0psi) @00:56:25.56 Checking for cartridge leaks from 29.9psia @00:56:25.59 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @00:56:30.69 Sampling 1000.0ml @00:56:30.70 SamplePump.setPosition! 0ml @00:56:30.75 Exhaust.open @00:56:31.46 Intake.open @00:56:32.29 Pressure.reconfigure :default @00:56:32.35 Pressure.hold @00:56:32.47 Sea 2.5m deep (18.4psia) @00:56:32.60 SamplePump.reconfigure :sample @00:56:32.67 SamplePump.seek 1000ml,1:07:09 @00:56:35.33 Cartridge 10 sampling 1/1000ml at 0.41ml/s with 13.3psi vacuum @00:57:35.33 Cartridge 10 sampling 22/1000ml at 0.35ml/s with 11.6psi vacuum 1.6m deep (17.1psia) @00:58:35.33 Cartridge 10 sampling 42/1000ml at 0.35ml/s with 13.3psi vacuum @00:59:35.33 Cartridge 10 sampling 63/1000ml at 0.32ml/s with 12.9psi vacuum @01:00:35.33 Cartridge 10 sampling 83/1000ml at 0.35ml/s with 12.1psi vacuum @01:01:35.33 Cartridge 10 sampling 103/1000ml at 0.33ml/s with 13.0psi vacuum @01:02:35.33 Cartridge 10 sampling 123/1000ml at 0.32ml/s with 13.4psi vacuum @01:03:35.33 Cartridge 10 sampling 142/1000ml at 0.30ml/s with 12.7psi vacuum @01:04:35.33 Cartridge 10 sampling 161/1000ml at 0.30ml/s with 12.1psi vacuum @01:05:35.33 Cartridge 10 sampling 180/1000ml at 0.33ml/s with 13.8psi vacuum @01:06:35.33 Cartridge 10 sampling 199/1000ml at 0.29ml/s with 11.3psi vacuum @01:07:35.33 Cartridge 10 sampling 218/1000ml at 0.29ml/s with 12.1psi vacuum @01:08:35.33 Cartridge 10 sampling 236/1000ml at 0.30ml/s with 11.3psi vacuum @01:09:35.33 Cartridge 10 sampling 254/1000ml at 0.31ml/s with 13.4psi vacuum @01:10:35.33 Cartridge 10 sampling 271/1000ml at 0.31ml/s with 14.9psi vacuum @01:11:35.33 Cartridge 10 sampling 289/1000ml at 0.28ml/s with 12.0psi vacuum @01:12:35.33 Cartridge 10 sampling 306/1000ml at 0.30ml/s with 12.2psi vacuum 1.6m deep (17.1psia) @01:13:35.33 Cartridge 10 sampling 323/1000ml at 0.26ml/s with 12.0psi vacuum @01:14:35.33 Cartridge 10 sampling 339/1000ml at 0.28ml/s with 11.2psi vacuum 1.6m deep (17.0psia) @01:15:35.33 Cartridge 10 sampling 355/1000ml at 0.26ml/s with 12.5psi vacuum @01:16:35.33 Cartridge 10 sampling 371/1000ml at 0.29ml/s with 13.0psi vacuum @01:17:35.33 Cartridge 10 sampling 387/1000ml at 0.26ml/s with 15.3psi vacuum @01:18:35.33 Cartridge 10 sampling 403/1000ml at 0.25ml/s with 11.4psi vacuum 2.5m deep (18.4psia) @01:19:35.33 Cartridge 10 sampling 419/1000ml at 0.26ml/s with 15.0psi vacuum @01:20:35.33 Cartridge 10 sampling 435/1000ml at 0.26ml/s with 12.9psi vacuum 1.8m deep (17.4psia) @01:21:35.33 Cartridge 10 sampling 450/1000ml at 0.25ml/s with 14.8psi vacuum @01:22:35.33 Cartridge 10 sampling 465/1000ml at 0.25ml/s with 12.0psi vacuum 1.6m deep (17.1psia) @01:23:35.33 Cartridge 10 sampling 481/1000ml at 0.25ml/s with 15.9psi vacuum @01:24:35.33 Cartridge 10 sampling 496/1000ml at 0.25ml/s with 13.3psi vacuum 1.8m deep (17.4psia) @01:25:10.06 Flow averaged 0.29ml/s over 28:33 @01:25:10.09 Waiting up to 7 seconds for vacuum to reduce from 14.7 to 10.0psi @01:25:10.79 Relieved excess vacuum in 5.3 seconds @01:25:10.81 SamplePump.seek 1000ml,33:33 @01:25:35.32 Cartridge 10 sampling 510/1000ml at 0.25ml/s with 15.3psi vacuum @01:26:03.74 Flow averaged 0.29ml/s over 45 seconds @01:26:03.77 Waiting up to 6 seconds for vacuum to reduce from 14.4 to 10.0psi @01:26:04.47 Relieved excess vacuum in 9 seconds @01:26:04.49 SamplePump.seek 1000ml,32:40 @01:26:12.45 Flow estimate reduced to 0.24ml/s @01:26:12.46 Intake.close @01:26:12.53 Exhaust.close @01:26:12.69 Toroid.reconfigure :hold @01:26:12.77 Toroid.seek :bypass @01:26:14.39 Toroid.reconfigure :default @01:26:14.46 Toroid.seek :clear @01:26:16.08 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @01:26:16.10 Pressure.coast @01:26:16.28 Sampled 519.3ml @01:26:18.35 -> Cmd.startProcessing @01:26:20.20 SPR.command \"read_sequences\" @01:26:20.38 SPR.command \"stats_on\" @01:26:20.42 SPR.command \"start 0 1\" @01:26:22.42 Begun log /var/log/esp/spr19jul2801h.spr @01:26:23.23 Handoff.select :waste @01:26:23.43 Archive.seek :empty @01:26:30.61 Syringe tare is 20% of full scale @01:26:41.16 PinPort.seek :cartridge @01:26:45.72 Lysis1.seek 1350ul @01:26:48.20 Lysis2.seek 1700ul @01:26:51.30 Plunge.seek :reagent @01:26:52.14 Evacuating puck with filter bubble point of 30psi @01:26:52.15 PinPort.seek :cartridge @01:26:52.23 Handoff.select :waste @01:26:59.56 Air pumping 1 strokes to reach 4psi @01:26:59.58 Air.reconfigure :gulp @01:26:59.67 Air.seek :tVent @01:27:01.46 0.1psi after gulp @01:27:01.47 Air stroke #1 of 1 @01:27:01.49 Air.reconfigure :pressurize @01:27:01.58 Air.seek :pressurized @01:27:14.42 0.2psi after pressurize @01:27:14.43 Air.reconfigure :compress @01:27:14.51 Air.seek :evac @01:27:17.18 0.7psi after compress @01:27:31.10 0.4psi after compress (stable) @01:27:31.22 at 0.4psi Opened to waste @01:27:31.23 Handoff.select :blocked @01:27:31.40 Air pumping 10 strokes to reach 30psi @01:27:31.42 Air.reconfigure :gulp @01:27:31.49 Air.seek :tVent @01:27:41.39 9.7psi after gulp @01:27:41.40 Air stroke #1 of 10 @01:27:41.42 Air.reconfigure :pressurize @01:27:41.49 Air.seek :pressurized @01:27:54.34 10.5psi after pressurize @01:27:54.35 Air.reconfigure :compress @01:27:54.43 Air.seek :evac @01:27:57.12 17.2psi after compress @01:28:15.07 26.4psi after compress (stable) @01:28:15.09 Air.reconfigure :gulp @01:28:15.16 Air.seek :tVent @01:28:25.06 26.6psi after gulp @01:28:25.07 Air stroke #2 of 10 @01:28:25.09 Air.reconfigure :pressurize @01:28:25.17 Air.seek :pressurized @01:28:38.05 25.9psi after pressurize @01:28:38.06 Air.reconfigure :compress @01:28:38.14 Air.seek :evac @01:28:40.17 Reached 30.4 of 30.0psi after pumping 3076ul (1.98 strokes) @01:28:40.18 Handoff.select :waste @01:29:03.55 delay 1:00 @01:30:03.58 Handoff.select :blocked @01:30:28.95 Handoff.select :waste @01:30:29.07 PSI at @1.29psi at evacEnd @01:30:29.08 Handoff.select :blocked @01:30:29.21 Air.reconfigure :gulp @01:30:29.29 Air.seek :tVent @01:30:46.15 Stable @1.1psi after Handoff blocked @01:30:46.24 Air pumping 10 strokes to reach 26psi @01:30:46.25 Air stroke #1 of 10 @01:30:46.27 Air.reconfigure :pressurize @01:30:46.34 Air.seek :pressurized @01:30:59.19 1.6psi after pressurize @01:30:59.20 Air.reconfigure :compress @01:30:59.28 Air.seek :evac @01:31:01.95 3.8psi after compress @01:31:25.26 12.4psi after compress (stable) @01:31:25.28 Air.reconfigure :gulp @01:31:25.38 Air.seek :tVent @01:31:35.33 13.2psi after gulp @01:31:35.34 Air stroke #2 of 10 @01:31:35.36 Air.reconfigure :pressurize @01:31:35.44 Air.seek :pressurized @01:31:48.29 13.5psi after pressurize @01:31:48.30 Air.reconfigure :compress @01:31:48.38 Air.seek :evac @01:31:51.05 15.1psi after compress @01:32:15.26 23.3psi after compress (stable) @01:32:15.28 Air.reconfigure :gulp @01:32:15.36 Air.seek :tVent @01:32:25.25 24.2psi after gulp @01:32:25.26 Air stroke #3 of 10 @01:32:25.28 Air.reconfigure :pressurize @01:32:25.36 Air.seek :pressurized @01:32:38.20 24.3psi after pressurize @01:32:38.23 Air.reconfigure :compress @01:32:38.31 Air.seek :evac @01:32:40.98 25.0psi after compress @01:32:41.99 Reached 26.2 of 26.1psi after pumping 6230ul (4.00 strokes) @01:32:42.01 Handoff.select :waste @01:32:49.91 delay 1:00 @01:33:49.94 Handoff.select :blocked @01:34:14.97 Handoff.select :waste @01:34:15.09 PSI at @0.72psi at evacEnd @01:34:15.10 Air.reconfigure :default @01:34:15.19 Air.seek :fVent @01:34:16.62 delay 5 seconds @01:34:21.64 Seawater evacuated, Presure above/below filter released. Lysis begins @01:34:21.71 Lysis1.reconfigure :slow @01:34:21.77 Lysis1.seek 1050ul @01:34:51.85 delay 5 seconds @01:34:56.87 Handoff.select :blocked @01:34:57.03 Lysis1.seek 450ul @01:35:57.07 delay 3 seconds @01:36:00.16 Air.reconfigure to mix into 1.37806 parts Lysis1 @01:36:00.68 Air.seek :evac @01:36:00.72 Lysis1.seek 300ul @01:36:15.85 Air.reconfigure :default @01:36:15.91 Air.seek :full @01:36:30.14 Lysis1.seek :empty @01:37:00.28 Lysis Delivered. Syringe pressure @39.4psi @01:37:00.33 PuckHeater.seek 85.7C @01:38:06.73 PuckHeater.hold 15:00,85.7C @01:53:06.31 Lysis complete. Syringe pressure @50.7psi @01:53:06.32 Handoff.select :blocked @01:53:06.42 Lysis1.reconfigure :halfSpeed @01:53:06.49 Lysis1.seek 200ul @01:53:10.26 delay 1 second @01:53:11.28 Lysis1.seek :empty @01:53:15.12 Initial AirS pressure @51.1psi @01:53:15.18 Lysis1.reconfigure :extract @01:53:15.25 Lysis1.seek 875ul,10:00 @01:53:16.48 Air pumping 10 strokes to reach 21psi @01:53:16.49 Air stroke #1 of 10 @01:53:16.51 Air.reconfigure :pressurize @01:53:16.59 Air.seek :pressurized @01:53:29.78 16.8psi after pressurize @01:53:29.80 Air.reconfigure :compress @01:53:29.88 Air.seek :evac @01:53:32.55 15.8psi after compress @01:53:32.73 Reached 23.6 of 21.0psi after pumping 3115ul (2.00 strokes) @01:53:33.44 Air pumping 8.0 strokes to reach 21psi @01:53:33.46 Air.reconfigure :gulp @01:53:33.54 Air.seek :tVent @01:53:43.44 16.0psi after gulp @01:53:43.45 Air stroke #1 of 8.0 @01:53:43.47 Air.reconfigure :pressurize @01:53:43.55 Air.seek :pressurized @01:53:56.39 16.2psi after pressurize @01:53:56.40 Air.reconfigure :compress @01:53:56.48 Air.seek :evac @01:53:59.14 15.9psi after compress @01:54:03.14 Air.reconfigure :default @01:54:03.20 Air.hold @01:54:03.37 Lysis1 at 875ul pressure @16.2psi @01:54:03.38 Lysis1.reconfigure :default @01:54:03.46 Lysis1.seek 900ul @01:54:04.23 Lysis1 at 900ul pressure(finPSI) @13.7psi @01:54:04.24 delay 1 second @01:54:05.35 Lysis1 at 900ul pressure @16.5psi @01:54:05.37 Air.reconfigure :halfSpeed @01:54:05.44 Air.seek :fVent @01:54:07.70 Puck Top Vented. Syringe pressure @1.4psi @01:54:07.71 Handoff.select :waste @01:54:07.82 delay 5 seconds @01:54:12.84 Lysate Recovered in LyS1 @01:54:12.85 Lysis1.reconfigure :DAmix @01:54:12.96 Lysis2.reconfigure :DAmix @01:54:13.36 Lysis1.seek 650ul @01:54:13.44 Lysis2.seek 1450ul @01:54:48.48 delay 5 seconds @01:54:53.50 Handoff.select SPR @01:54:53.61 SPR.command \"start 2 14\" @01:54:54.35 delay 10 seconds @01:55:04.37 SPR.command \"start 1 0\" @01:55:08.07 Waiting up to 4:00 for SPR to enter state 1 @01:57:41.17 SPR.command \"hold_on\" @01:57:43.07 SPR Dilution 1 of 1 -- Running Experiment 1 @01:57:43.47 Lysis1.seek 350ul @01:57:43.51 Lysis2.seek 1150ul @01:58:25.54 volL1 = 300.0 and volL2 = 300.0 @01:58:25.55 delay 15 seconds @01:58:40.58 SPR.command \"hold_off\" @01:58:43.25 Waiting up to 25:00 for SPR to enter state 7 @02:18:56.14 SPR Analysis has Completed @02:18:56.15 Handoff.select :waste @02:18:57.08 Ended log /var/log/esp/spr19jul2801h.spr @02:18:57.09 SPR.command \"stats_off\" @02:18:58.62 -> Cmd.stop @02:18:58.71 Handoff.select :close @02:18:58.84 Gate.power :heat,:OFF @02:18:58.86 Gate.power :cartridge,:OFF @02:18:58.94 Carousel.seek 10 @02:18:59.11 Plunge.seek :locked @02:18:59.94 PinPort.seek :clear @02:19:04.55 Archive.seek :clear @02:19:04.67 Air.reconfigure :default @02:19:04.80 Lysis1.reconfigure :default @02:19:04.92 Air.seek :clear @02:19:04.98 Lysis1.seek :clear @02:19:05.06 Lysis2.reconfigure :default @02:19:05.13 Lysis2.seek :clear @02:19:22.27 Archive.coast @02:19:22.32 Air.coast @02:19:22.37 Lysis1.coast @02:19:22.42 Lysis2.coast @02:19:22.47 Plunge.coast @02:19:22.52 SamplePump.coast @02:19:22.92 Toroid.coast @02:19:22.97 Carousel.coast @02:19:23.02 PinPort.coast @02:19:23.20 Gate.power :motor,:OFF @02:19:23.22 Gate.power :core,:OFF @02:19:23.24 Gate.power :analytic1,:OFF @02:19:23.26 Gate.power :analytic2,:OFF @02:19:23.28 Gate.power :analytic3,:OFF @02:19:23.30 Gate.power :main,:OFF @02:19:23.32 Safely stopped and ready to power off ##-- file closed 2019-07-28T13:08:35.482Z ##-- file opened 2019-07-28T13:08:35.484Z @06:08:35.65 -> showStatus @06:08:37.66 -> Cmd.loadCartridge -9 @06:08:37.79 Gate.power :main,:ON,limit to 5000mA @06:08:37.80 Gate.power :analytic1,:ON,limit to 5000mA @06:08:37.82 Gate.power :analytic2,:ON,limit to 5000mA @06:08:37.84 Gate.power :analytic3,:ON,limit to 5000mA @06:08:37.98 Powering core bus @06:08:40.25 SPR Module 1, FW Rev. B, FPGA Rev. C @06:08:40.39 Archive.configure :default @06:08:40.51 Air.configure :default @06:08:40.62 Lysis1.configure :default @06:08:40.72 Lysis2.configure :default @06:08:40.84 Plunge.configure :default @06:08:40.98 SamplePump.configure :default @06:08:41.11 ExtraPump.configure :default @06:08:41.23 Toroid.configure :default @06:08:41.34 Carousel.configure :default @06:08:41.46 Pressure.configure :default @06:08:41.56 PinPort.configure :default @06:08:41.62 All elf controllers running firmware version 4.58 @06:08:41.64 Gate.power :motor,:ON,limit to 5000mA @06:08:41.76 Archive.reconfigure :default @06:08:42.09 Archive.home.jog 0 @06:08:42.22 Toroid.reconfigure :default @06:08:42.30 Plunge.reconfigure :default @06:08:42.34 Lysis1.reconfigure :default @06:08:42.38 PinPort.reconfigure :default @06:08:42.43 Air.reconfigure :default @06:08:42.48 Lysis2.reconfigure :default @06:08:42.70 Air.home.jog 0 @06:08:42.74 Toroid.home.jog 0 @06:08:42.80 Plunge.home.jog 0 @06:08:42.86 Lysis1.home.jog 0 @06:08:42.92 Lysis2.home.jog 0 @06:08:42.98 PinPort.home.jog 0 @06:08:43.28 Archive.reconfigure :homing @06:08:43.46 PinPort.reconfigure :homing @06:08:43.54 Toroid.reconfigure :homing @06:08:43.58 Lysis1.reconfigure :homing @06:08:43.61 Air.reconfigure :homing @06:08:43.65 Lysis2.reconfigure :homing @06:08:43.70 Plunge.reconfigure :homing @06:08:43.89 Plunge.jog 1547 @06:08:43.95 Lysis1.jog 1407 @06:08:44.00 Archive.jog 1408 @06:08:44.06 Lysis2.jog 162 @06:08:44.11 PinPort.jog -106 @06:08:44.17 Toroid.jog 634 @06:08:44.23 Air.jog 1412 @06:08:44.34 PinPort.setRawPosition! 0 @06:08:44.38 PinPort.seek :home @06:08:44.48 PinPort.reconfigure :default @06:08:44.55 Lysis2.setRawPosition! 50 @06:08:44.59 Lysis2.seek :home @06:08:44.87 Lysis2.reconfigure :default @06:08:45.11 Plunge.setRawPosition! 50 @06:08:45.13 Lysis1.setRawPosition! 50 @06:08:45.22 Lysis1.seek :home @06:08:45.25 Plunge.seek :home @06:08:45.42 Air.setRawPosition! 50 @06:08:45.44 Archive.setRawPosition! 50 @06:08:45.52 Air.seek :home @06:08:45.56 Archive.seek :home @06:08:45.69 Lysis1.reconfigure :default @06:08:45.72 Plunge.reconfigure :default @06:08:45.87 Air.reconfigure :default @06:08:45.95 Archive.reconfigure :default @06:08:46.33 Toroid.setRawPosition! 0 @06:08:46.37 Toroid.seek :home @06:08:46.46 Toroid.reconfigure :default @06:08:46.58 Toroid.seek :clear @06:08:48.21 Carousel.reconfigure :default @06:08:48.28 Carousel.home.jog 0 @06:08:48.78 Carousel.seek 10 @06:08:48.94 Selecting Cartridge 9 @06:08:49.18 Carousel.seek 9 @06:08:49.67 Lysis1.seek :locked @06:08:53.79 Plunge.seek :locked @06:08:53.91 Air.seek :locked @06:08:58.04 Powering cartridge bus @06:09:00.17 Gate.setIR 1750,5000,0,6 @06:09:00.30 Gate.setIR 0,5000,0,6 @06:09:00.35 Loaded Lyse-n-Go Cartridge @06:09:00.37 PuckHeater.configure HeatConfig @06:09:00.42 Gate.power :heat,:ON,limit to 5000mA @06:09:16.47 -> Cmd.startFiltering @06:09:16.69 Duration of filtering limited to 2:05:00 @06:09:16.95 Priming sample loop w/100ml, bypass w/10ml @06:09:16.96 SamplePump.setPosition! 0ml @06:09:17.01 Bypass.open @06:09:18.49 Intake.open @06:09:19.30 Sea 4.0m deep (20.6psia) @06:09:19.32 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:09:22.15 Vacuum tare is 52% of full scale @06:09:22.64 Pressure.reconfigure :default @06:09:22.70 Pressure.hold @06:09:22.87 SamplePump.reconfigure :prime @06:09:22.95 SamplePump.seek 10ml,1:10 @06:09:31.92 Pressure.coast @06:09:31.97 SamplePump.setPosition! 0ml @06:09:32.01 Exhaust.open @06:09:33.45 Bypass.close @06:09:34.97 Pressure.reconfigure :default @06:09:35.03 Pressure.hold @06:09:35.15 Sea 4.2m deep (21.0psia) @06:09:35.27 SamplePump.reconfigure :prime @06:09:35.35 SamplePump.seek 100ml,7:10 @06:11:31.04 Pressure.coast @06:11:31.10 Bypass.open @06:11:31.80 Intake.close @06:11:32.63 Toroid.reconfigure :hold @06:11:32.69 Toroid.seek :crack @06:11:33.49 Toroid.reconfigure :default @06:11:33.56 Toroid.seek :clear @06:11:35.11 Toroid.reconfigure :hold @06:11:35.18 Toroid.seek :cartridge @06:11:36.98 Toroid.reconfigure :default @06:11:37.04 Toroid.seek :clear @06:11:39.00 Pressurizing cartridge to 14psi above 17.8psia @06:11:39.01 SamplePump.setPosition! 0ml @06:11:39.05 Intake.open @06:11:39.77 Bypass.close @06:11:40.48 Exhaust.close @06:11:41.29 Pressure.reconfigure :default @06:11:41.34 Pressure.hold @06:11:41.51 SamplePump.reconfigure :charge @06:11:41.58 SamplePump.seek 2.3ml,41 seconds @06:11:51.22 Pressure.coast @06:11:51.28 Intake.close @06:11:52.07 Pressurized cartridge with 0.434ml to 14.0psi @06:11:52.08 Isolated @30.0psia (12.2psi) @06:11:52.09 Checking for cartridge leaks from 30.0psia @06:11:52.12 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @06:11:57.22 Sampling 1000.0ml @06:11:57.24 SamplePump.setPosition! 0ml @06:11:57.29 Exhaust.open @06:11:58.02 Intake.open @06:11:58.84 Pressure.reconfigure :default @06:11:58.89 Pressure.hold @06:11:59.00 Sea 3.3m deep (19.6psia) @06:11:59.14 SamplePump.reconfigure :sample @06:11:59.21 SamplePump.seek 1000ml,1:07:09 @06:12:01.88 Cartridge 9 sampling 1/1000ml at 0.45ml/s with 12.8psi vacuum 1.6m deep (17.1psia) @06:13:01.88 Cartridge 9 sampling 22/1000ml at 0.32ml/s with 12.1psi vacuum 2.1m deep (17.7psia) @06:14:01.88 Cartridge 9 sampling 42/1000ml at 0.35ml/s with 12.6psi vacuum 1.7m deep (17.2psia) @06:15:01.88 Cartridge 9 sampling 62/1000ml at 0.32ml/s with 13.7psi vacuum @06:16:01.88 Cartridge 9 sampling 82/1000ml at 0.33ml/s with 12.3psi vacuum @06:17:01.88 Cartridge 9 sampling 102/1000ml at 0.32ml/s with 12.3psi vacuum 1.6m deep (17.1psia) @06:18:01.88 Cartridge 9 sampling 122/1000ml at 0.32ml/s with 11.9psi vacuum @06:19:01.88 Cartridge 9 sampling 143/1000ml at 0.32ml/s with 12.2psi vacuum @06:20:01.88 Cartridge 9 sampling 162/1000ml at 0.35ml/s with 11.3psi vacuum 2.0m deep (17.7psia) @06:21:01.88 Cartridge 9 sampling 182/1000ml at 0.35ml/s with 13.6psi vacuum @06:22:01.88 Cartridge 9 sampling 202/1000ml at 0.32ml/s with 14.8psi vacuum @06:23:01.88 Cartridge 9 sampling 222/1000ml at 0.31ml/s with 12.8psi vacuum @06:24:01.89 Cartridge 9 sampling 242/1000ml at 0.34ml/s with 16.0psi vacuum @06:25:01.88 Cartridge 9 sampling 261/1000ml at 0.33ml/s with 10.2psi vacuum 1.9m deep (17.5psia) @06:26:01.88 Cartridge 9 sampling 281/1000ml at 0.32ml/s with 15.6psi vacuum @06:27:01.88 Cartridge 9 sampling 301/1000ml at 0.32ml/s with 11.6psi vacuum @06:28:01.88 Cartridge 9 sampling 320/1000ml at 0.32ml/s with 12.3psi vacuum @06:29:01.88 Cartridge 9 sampling 340/1000ml at 0.32ml/s with 12.7psi vacuum @06:30:01.88 Cartridge 9 sampling 359/1000ml at 0.30ml/s with 13.4psi vacuum @06:31:01.88 Cartridge 9 sampling 378/1000ml at 0.33ml/s with 12.9psi vacuum 1.7m deep (17.1psia) @06:32:01.88 Cartridge 9 sampling 398/1000ml at 0.31ml/s with 15.3psi vacuum @06:33:01.88 Cartridge 9 sampling 417/1000ml at 0.31ml/s with 11.7psi vacuum 1.7m deep (17.2psia) @06:34:01.87 Cartridge 9 sampling 436/1000ml at 0.31ml/s with 11.7psi vacuum @06:35:01.88 Cartridge 9 sampling 455/1000ml at 0.30ml/s with 10.0psi vacuum 2.0m deep (17.6psia) @06:36:01.88 Cartridge 9 sampling 474/1000ml at 0.33ml/s with 13.0psi vacuum 1.6m deep (17.0psia) @06:37:01.88 Cartridge 9 sampling 493/1000ml at 0.33ml/s with 13.5psi vacuum @06:38:01.88 Cartridge 9 sampling 512/1000ml at 0.30ml/s with 15.6psi vacuum @06:39:01.87 Cartridge 9 sampling 531/1000ml at 0.33ml/s with 13.8psi vacuum @06:40:01.88 Cartridge 9 sampling 549/1000ml at 0.31ml/s with 13.2psi vacuum @06:41:01.88 Cartridge 9 sampling 568/1000ml at 0.32ml/s with 14.7psi vacuum 2.2m deep (18.0psia) @06:42:01.88 Cartridge 9 sampling 586/1000ml at 0.32ml/s with 14.2psi vacuum @06:43:02.24 Cartridge 9 sampling 605/1000ml at 0.33ml/s with 11.8psi vacuum 2.0m deep (17.6psia) @06:44:01.87 Cartridge 9 sampling 623/1000ml at 0.30ml/s with 11.9psi vacuum 1.8m deep (17.4psia) @06:45:01.88 Cartridge 9 sampling 641/1000ml at 0.32ml/s with 10.8psi vacuum @06:46:01.88 Cartridge 9 sampling 660/1000ml at 0.30ml/s with 12.9psi vacuum @06:47:01.88 Cartridge 9 sampling 678/1000ml at 0.28ml/s with 14.1psi vacuum @06:48:01.88 Cartridge 9 sampling 696/1000ml at 0.27ml/s with 13.6psi vacuum @06:49:01.87 Cartridge 9 sampling 713/1000ml at 0.31ml/s with 15.4psi vacuum @06:50:01.88 Cartridge 9 sampling 731/1000ml at 0.32ml/s with 12.9psi vacuum @06:51:01.88 Cartridge 9 sampling 749/1000ml at 0.30ml/s with 16.1psi vacuum @06:52:01.88 Cartridge 9 sampling 766/1000ml at 0.29ml/s with 15.5psi vacuum @06:53:01.88 Cartridge 9 sampling 784/1000ml at 0.29ml/s with 13.4psi vacuum @06:54:01.87 Cartridge 9 sampling 801/1000ml at 0.30ml/s with 15.1psi vacuum @06:55:01.88 Cartridge 9 sampling 818/1000ml at 0.31ml/s with 13.6psi vacuum @06:56:01.88 Cartridge 9 sampling 835/1000ml at 0.28ml/s with 11.6psi vacuum 2.2m deep (18.0psia) @06:57:01.88 Cartridge 9 sampling 852/1000ml at 0.30ml/s with 11.6psi vacuum 1.9m deep (17.6psia) @06:58:01.88 Cartridge 9 sampling 869/1000ml at 0.30ml/s with 13.4psi vacuum 1.6m deep (17.0psia) @06:59:01.87 Cartridge 9 sampling 886/1000ml at 0.28ml/s with 11.9psi vacuum @07:00:01.88 Cartridge 9 sampling 903/1000ml at 0.27ml/s with 16.5psi vacuum @07:01:01.88 Cartridge 9 sampling 920/1000ml at 0.26ml/s with 12.2psi vacuum @07:02:01.88 Cartridge 9 sampling 936/1000ml at 0.26ml/s with 10.5psi vacuum @07:03:01.88 Cartridge 9 sampling 953/1000ml at 0.27ml/s with 16.1psi vacuum @07:04:01.87 Cartridge 9 sampling 969/1000ml at 0.28ml/s with 13.0psi vacuum @07:05:01.88 Cartridge 9 sampling 986/1000ml at 0.27ml/s with 13.6psi vacuum @07:05:55.25 Pressure.coast @07:05:55.36 Intake.close @07:05:55.40 Exhaust.close @07:05:55.55 Toroid.reconfigure :hold @07:05:55.61 Toroid.seek :bypass @07:05:57.23 Toroid.reconfigure :default @07:05:57.29 Toroid.seek :clear @07:05:58.91 Sampled 1000.0ml @07:06:00.26 -> Cmd.startProcessing @07:06:02.12 SPR.command \"read_sequences\" @07:06:02.15 SPR.command \"stats_on\" @07:06:02.19 SPR.command \"start 0 1\" @07:06:04.20 Begun log /var/log/esp/spr19jul2807h.spr @07:06:04.88 Handoff.select :waste @07:06:05.06 Archive.seek :empty @07:06:12.22 Syringe tare is 21% of full scale @07:06:22.79 PinPort.seek :cartridge @07:06:27.35 Lysis1.seek 1350ul @07:06:29.84 Lysis2.seek 1700ul @07:06:32.93 Plunge.seek :reagent @07:06:33.77 Evacuating puck with filter bubble point of 30psi @07:06:33.78 PinPort.seek :cartridge @07:06:33.87 Handoff.select :waste @07:06:41.19 Air pumping 1 strokes to reach 4psi @07:06:41.21 Air.reconfigure :gulp @07:06:41.30 Air.seek :tVent @07:06:43.10 -0.1psi after gulp @07:06:43.11 Air stroke #1 of 1 @07:06:43.14 Air.reconfigure :pressurize @07:06:43.22 Air.seek :pressurized @07:06:56.06 -0.1psi after pressurize @07:06:56.07 Air.reconfigure :compress @07:06:56.15 Air.seek :evac @07:06:58.81 0.3psi after compress @07:07:13.10 0.1psi after compress (stable) @07:07:13.22 at 0.1psi Opened to waste @07:07:13.23 Handoff.select :blocked @07:07:13.43 Air pumping 10 strokes to reach 30psi @07:07:13.45 Air.reconfigure :gulp @07:07:13.53 Air.seek :tVent @07:07:23.42 6.0psi after gulp @07:07:23.43 Air stroke #1 of 10 @07:07:23.45 Air.reconfigure :pressurize @07:07:23.53 Air.seek :pressurized @07:07:36.37 8.3psi after pressurize @07:07:36.38 Air.reconfigure :compress @07:07:36.46 Air.seek :evac @07:07:39.13 9.6psi after compress @07:07:51.93 Reached 30.0 of 30.0psi after pumping 3115ul (2.00 strokes) @07:07:51.94 Handoff.select :waste @07:08:00.93 delay 1:00 @07:09:00.96 Handoff.select :blocked @07:09:15.54 Pressure too High, open to waste again (4.1psi) @07:09:15.55 Handoff.select :waste @07:09:15.68 delay 1:00 @07:10:15.70 Handoff.select :blocked @07:10:40.71 Handoff.select :waste @07:10:40.83 PSI at @1.33psi at evacEnd @07:10:40.85 Handoff.select :blocked @07:10:40.98 Air.reconfigure :gulp @07:10:41.04 Air.seek :tVent @07:10:58.11 Stable @2.0psi after Handoff blocked @07:10:58.19 Air pumping 10 strokes to reach 27psi @07:10:58.20 Air stroke #1 of 10 @07:10:58.22 Air.reconfigure :pressurize @07:10:58.29 Air.seek :pressurized @07:11:11.14 2.4psi after pressurize @07:11:11.15 Air.reconfigure :compress @07:11:11.23 Air.seek :evac @07:11:13.89 2.5psi after compress @07:12:12.37 10.8psi after compress (stable) @07:12:12.39 Air.reconfigure :gulp @07:12:12.46 Air.seek :tVent @07:12:22.37 12.9psi after gulp @07:12:22.38 Air stroke #2 of 10 @07:12:22.40 Air.reconfigure :pressurize @07:12:22.47 Air.seek :pressurized @07:12:35.31 12.7psi after pressurize @07:12:35.32 Air.reconfigure :compress @07:12:35.40 Air.seek :evac @07:12:38.06 12.5psi after compress @07:13:34.40 Reached 27.1 of 27.0psi after pumping 4672ul (3.00 strokes) @07:13:34.41 Handoff.select :waste @07:13:48.91 delay 1:00 @07:14:48.93 Handoff.select :blocked @07:15:03.55 Pressure too High, open to waste again (4.1psi) @07:15:03.56 Handoff.select :waste @07:15:03.68 delay 1:00 @07:16:03.70 Handoff.select :blocked @07:16:29.09 Handoff.select :waste @07:16:29.21 PSI at @2.18psi at evacEnd @07:16:29.22 Air.reconfigure :default @07:16:29.31 Air.seek :fVent @07:16:30.75 delay 5 seconds @07:16:35.77 Seawater evacuated, Presure above/below filter released. Lysis begins @07:16:35.84 Lysis1.reconfigure :slow @07:16:35.91 Lysis1.seek 1050ul @07:17:05.95 delay 5 seconds @07:17:10.97 Handoff.select :blocked @07:17:11.14 Lysis1.seek 450ul @07:18:11.16 delay 3 seconds @07:18:14.23 Air.reconfigure to mix into 1.37806 parts Lysis1 @07:18:14.65 Air.seek :evac @07:18:14.69 Lysis1.seek 300ul @07:18:29.80 Air.reconfigure :default @07:18:29.87 Air.seek :full @07:18:44.08 Lysis1.seek :empty @07:19:14.20 Lysis Delivered. Syringe pressure @44.8psi @07:19:14.24 PuckHeater.seek 85.8C @07:20:24.76 PuckHeater.hold 15:00,85.8C @07:35:23.27 Lysis complete. Syringe pressure @41.3psi @07:35:23.28 Handoff.select :blocked @07:35:23.38 Lysis1.reconfigure :halfSpeed @07:35:23.44 Lysis1.seek 200ul @07:35:27.21 delay 1 second @07:35:28.23 Lysis1.seek :empty @07:35:32.05 Initial AirS pressure @42.4psi @07:35:32.12 Lysis1.reconfigure :extract @07:35:32.22 Lysis1.seek 875ul,10:00 @07:35:33.37 Air pumping 10 strokes to reach 21psi @07:35:33.38 Air stroke #1 of 10 @07:35:33.75 Air.reconfigure :pressurize @07:35:33.83 Air.seek :pressurized @07:35:46.66 11.3psi after pressurize @07:35:46.67 Air.reconfigure :compress @07:35:46.76 Air.seek :evac @07:35:49.42 17.8psi after compress @07:36:03.34 15.8psi after compress (stable) @07:36:03.37 Air.reconfigure :gulp @07:36:03.44 Air.seek :tVent @07:36:13.34 15.8psi after gulp @07:36:13.35 Air stroke #2 of 10 @07:36:13.37 Air.reconfigure :pressurize @07:36:13.45 Air.seek :pressurized @07:36:26.28 15.8psi after pressurize @07:36:26.29 Air.reconfigure :compress @07:36:26.37 Air.seek :evac @07:36:29.04 16.0psi after compress @07:36:42.95 15.9psi after compress (stable) @07:36:42.97 Air.reconfigure :gulp @07:36:43.05 Air.seek :tVent @07:36:52.97 15.4psi after gulp @07:36:52.98 Air stroke #3 of 10 @07:36:52.99 Air.reconfigure :pressurize @07:36:53.08 Air.seek :pressurized @07:37:05.92 15.5psi after pressurize @07:37:05.93 Air.reconfigure :compress @07:37:06.01 Air.seek :evac @07:37:08.69 16.5psi after compress @07:37:22.61 15.8psi after compress (stable) @07:37:22.63 Air.reconfigure :gulp @07:37:22.71 Air.seek :tVent @07:37:26.06 Air.reconfigure :default @07:37:26.11 Air.hold @07:37:26.91 Lysis1 at 875ul pressure @16.7psi @07:37:26.93 Lysis1.reconfigure :default @07:37:27.00 Lysis1.seek 900ul @07:37:27.77 Lysis1 at 900ul pressure(finPSI) @11.1psi @07:37:27.78 delay 1 second @07:37:28.89 Lysis1 at 900ul pressure @13.0psi @07:37:28.91 Air.reconfigure :halfSpeed @07:37:28.98 Air.seek :fVent @07:37:41.29 Puck Top Vented. Syringe pressure @2.6psi @07:37:41.30 Handoff.select :waste @07:37:41.42 delay 5 seconds @07:37:46.53 Lysate Recovered in LyS1 @07:37:46.55 Lysis1.reconfigure :DAmix @07:37:46.66 Lysis2.reconfigure :DAmix @07:37:46.98 Lysis1.seek 650ul @07:37:47.05 Lysis2.seek 1450ul @07:38:22.09 delay 5 seconds @07:38:27.11 Handoff.select SPR @07:38:27.25 SPR.command \"start 2 14\" @07:38:28.67 delay 10 seconds @07:38:38.69 SPR.command \"start 1 0\" @07:38:41.92 Waiting up to 4:00 for SPR to enter state 1 @07:41:14.54 SPR.command \"hold_on\" @07:41:15.97 SPR Dilution 1 of 1 -- Running Experiment 1 @07:41:16.38 Lysis1.seek 350ul @07:41:16.42 Lysis2.seek 1150ul @07:41:58.48 volL1 = 300.0 and volL2 = 300.0 @07:41:58.49 delay 15 seconds @07:42:13.51 SPR.command \"hold_off\" @07:42:16.62 Waiting up to 25:00 for SPR to enter state 7 @08:02:30.46 SPR Analysis has Completed @08:02:30.48 Handoff.select :waste @08:02:31.40 Ended log /var/log/esp/spr19jul2807h.spr @08:02:31.41 SPR.command \"stats_off\" @08:02:33.03 -> Cmd.stop @08:02:33.12 Handoff.select :close @08:02:33.24 Gate.power :heat,:OFF @08:02:33.26 Gate.power :cartridge,:OFF @08:02:33.34 Carousel.seek 9 @08:02:33.51 Plunge.seek :locked @08:02:34.34 PinPort.seek :clear @08:02:38.95 Archive.seek :clear @08:02:39.07 Air.reconfigure :default @08:02:39.21 Lysis1.reconfigure :default @08:02:39.32 Air.seek :clear @08:02:39.38 Lysis1.seek :clear @08:02:39.46 Lysis2.reconfigure :default @08:02:39.53 Lysis2.seek :clear @08:02:56.69 Archive.coast @08:02:56.74 Air.coast @08:02:56.79 Lysis1.coast @08:02:56.84 Lysis2.coast @08:02:56.89 Plunge.coast @08:02:57.27 SamplePump.coast @08:02:57.33 Toroid.coast @08:02:57.38 Carousel.coast @08:02:57.43 PinPort.coast @08:02:57.62 Gate.power :motor,:OFF @08:02:57.63 Gate.power :core,:OFF @08:02:57.66 Gate.power :analytic1,:OFF @08:02:57.68 Gate.power :analytic2,:OFF @08:02:57.70 Gate.power :analytic3,:OFF @08:02:57.72 Gate.power :main,:OFF @08:02:57.74 Safely stopped and ready to power off ##-- file closed 2019-07-28T15:11:55.064Z ##-- file opened 2019-07-28T15:11:55.066Z @08:11:54.88 -> showStatus @08:11:56.90 -> Cmd.loadCartridge -9 @08:11:57.03 Gate.power :main,:ON,limit to 5000mA @08:11:57.04 Gate.power :analytic1,:ON,limit to 5000mA @08:11:57.06 Gate.power :analytic2,:ON,limit to 5000mA @08:11:57.08 Gate.power :analytic3,:ON,limit to 5000mA @08:11:57.21 Powering core bus @08:11:59.50 SPR Module 1, FW Rev. B, FPGA Rev. C @08:11:59.62 Archive.configure :default @08:11:59.74 Air.configure :default @08:11:59.88 Lysis1.configure :default @08:11:59.98 Lysis2.configure :default @08:12:00.10 Plunge.configure :default @08:12:00.21 SamplePump.configure :default @08:12:00.33 ExtraPump.configure :default @08:12:00.45 Toroid.configure :default @08:12:00.57 Carousel.configure :default @08:12:00.69 Pressure.configure :default @08:12:00.80 PinPort.configure :default @08:12:00.86 All elf controllers running firmware version 4.58 @08:12:00.88 Gate.power :motor,:ON,limit to 5000mA @08:12:01.00 Archive.reconfigure :default @08:12:01.34 Archive.home.jog 0 @08:12:01.48 Toroid.reconfigure :default @08:12:01.56 Plunge.reconfigure :default @08:12:01.59 Lysis2.reconfigure :default @08:12:01.63 Air.reconfigure :default @08:12:01.66 PinPort.reconfigure :default @08:12:01.72 Lysis1.reconfigure :default @08:12:01.97 Lysis1.home.jog 0 @08:12:02.02 Plunge.home.jog 0 @08:12:02.07 PinPort.home.jog 0 @08:12:02.13 Toroid.home.jog 0 @08:12:02.19 Air.home.jog 0 @08:12:02.25 Lysis2.home.jog 0 @08:12:02.52 Archive.reconfigure :homing @08:12:02.81 Toroid.reconfigure :homing @08:12:02.85 Plunge.reconfigure :homing @08:12:02.88 Air.reconfigure :homing @08:12:02.91 PinPort.reconfigure :homing @08:12:02.95 Lysis1.reconfigure :homing @08:12:02.99 Lysis2.reconfigure :homing @08:12:03.21 Air.jog 152 @08:12:03.27 Lysis1.jog 1407 @08:12:03.32 PinPort.jog -106 @08:12:03.38 Plunge.jog 1557 @08:12:03.44 Toroid.jog 629 @08:12:03.49 Archive.jog 1412 @08:12:03.56 Lysis2.jog 162 @08:12:03.65 PinPort.setRawPosition! 0 @08:12:03.69 PinPort.seek :home @08:12:03.78 Air.setRawPosition! 50 @08:12:03.80 PinPort.reconfigure :default @08:12:03.87 Air.seek :home @08:12:04.00 Lysis2.setRawPosition! 50 @08:12:04.05 Lysis2.seek :home @08:12:04.18 Air.reconfigure :default @08:12:04.33 Lysis2.reconfigure :default @08:12:04.43 Lysis1.setRawPosition! 50 @08:12:04.49 Lysis1.seek :home @08:12:04.60 Plunge.setRawPosition! 50 @08:12:04.65 Plunge.seek :home @08:12:04.73 Archive.setRawPosition! 50 @08:12:04.79 Archive.seek :home @08:12:04.88 Lysis1.reconfigure :default @08:12:04.96 Plunge.reconfigure :default @08:12:05.07 Archive.reconfigure :default @08:12:05.57 Toroid.setRawPosition! 0 @08:12:05.61 Toroid.seek :home @08:12:05.70 Toroid.reconfigure :default @08:12:05.83 Toroid.seek :clear @08:12:07.45 Carousel.reconfigure :default @08:12:07.52 Carousel.home.jog 0 @08:12:08.02 Carousel.seek 9 @08:12:08.16 Selecting Cartridge 8 @08:12:08.40 Carousel.seek 8 @08:12:08.88 Lysis1.seek :locked @08:12:13.01 Plunge.seek :locked @08:12:13.13 Air.seek :locked @08:12:17.26 Powering cartridge bus @08:12:19.38 Gate.setIR 1750,5000,0,6 @08:12:19.51 Gate.setIR 0,5000,0,6 @08:12:19.56 Loaded Lyse-n-Go Cartridge @08:12:19.58 PuckHeater.configure HeatConfig @08:12:19.63 Gate.power :heat,:ON,limit to 5000mA @08:12:35.68 -> Cmd.startFiltering @08:12:35.90 Duration of filtering limited to 2:05:00 @08:12:36.16 Priming sample loop w/100ml, bypass w/10ml @08:12:36.17 SamplePump.setPosition! 0ml @08:12:36.21 Bypass.open @08:12:37.70 Intake.open @08:12:38.50 Sea 2.5m deep (18.3psia) @08:12:38.52 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:12:41.36 Vacuum tare is 52% of full scale @08:12:41.85 Pressure.reconfigure :default @08:12:41.91 Pressure.hold @08:12:42.08 SamplePump.reconfigure :prime @08:12:42.16 SamplePump.seek 10ml,1:10 @08:12:51.11 Pressure.coast @08:12:51.17 SamplePump.setPosition! 0ml @08:12:51.22 Exhaust.open @08:12:52.62 Bypass.close @08:12:54.12 Pressure.reconfigure :default @08:12:54.18 Pressure.hold @08:12:54.30 Sea 2.2m deep (17.9psia) @08:12:54.43 SamplePump.reconfigure :prime @08:12:54.51 SamplePump.seek 100ml,7:10 @08:14:50.09 Pressure.coast @08:14:50.14 Bypass.open @08:14:50.81 Intake.close @08:14:51.63 Toroid.reconfigure :hold @08:14:51.70 Toroid.seek :crack @08:14:52.49 Toroid.reconfigure :default @08:14:52.55 Toroid.seek :clear @08:14:54.11 Toroid.reconfigure :hold @08:14:54.18 Toroid.seek :cartridge @08:14:55.99 Toroid.reconfigure :default @08:14:56.05 Toroid.seek :clear @08:14:58.02 Pressurizing cartridge to 14psi above 18.3psia @08:14:58.03 SamplePump.setPosition! 0ml @08:14:58.07 Intake.open @08:14:58.80 Bypass.close @08:14:59.51 Exhaust.close @08:15:00.32 Pressure.reconfigure :default @08:15:00.37 Pressure.hold @08:15:00.54 SamplePump.reconfigure :charge @08:15:00.61 SamplePump.seek 2.3ml,41 seconds @08:15:09.64 Pressure.coast @08:15:09.69 Intake.close @08:15:10.52 Pressurized cartridge with 0.381ml to 14.5psi @08:15:10.53 Isolated @30.7psia (12.4psi) @08:15:10.54 Checking for cartridge leaks from 30.7psia @08:15:10.57 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @08:15:15.70 Sampling 1000.0ml @08:15:15.72 SamplePump.setPosition! 0ml @08:15:15.77 Exhaust.open @08:15:16.47 Intake.open @08:15:17.33 Pressure.reconfigure :default @08:15:17.39 Pressure.hold @08:15:17.50 Sea 2.9m deep (19.0psia) @08:15:17.63 SamplePump.reconfigure :sample @08:15:17.70 SamplePump.seek 1000ml,1:07:09 @08:15:20.37 Cartridge 8 sampling 1/1000ml at 0.36ml/s with 12.3psi vacuum @08:16:20.37 Cartridge 8 sampling 22/1000ml at 0.35ml/s with 13.9psi vacuum @08:17:20.37 Cartridge 8 sampling 43/1000ml at 0.35ml/s with 13.2psi vacuum @08:18:20.37 Cartridge 8 sampling 64/1000ml at 0.35ml/s with 11.7psi vacuum 2.1m deep (17.8psia) @08:19:20.37 Cartridge 8 sampling 85/1000ml at 0.35ml/s with 13.8psi vacuum @08:20:20.37 Cartridge 8 sampling 106/1000ml at 0.34ml/s with 13.2psi vacuum @08:21:20.37 Cartridge 8 sampling 127/1000ml at 0.33ml/s with 12.0psi vacuum 1.9m deep (17.5psia) @08:22:20.37 Cartridge 8 sampling 147/1000ml at 0.35ml/s with 14.0psi vacuum @08:23:20.37 Cartridge 8 sampling 168/1000ml at 0.34ml/s with 15.0psi vacuum @08:24:20.37 Cartridge 8 sampling 188/1000ml at 0.33ml/s with 14.6psi vacuum @08:25:20.37 Cartridge 8 sampling 208/1000ml at 0.34ml/s with 12.6psi vacuum @08:26:20.37 Cartridge 8 sampling 229/1000ml at 0.35ml/s with 14.5psi vacuum @08:27:20.37 Cartridge 8 sampling 249/1000ml at 0.34ml/s with 13.1psi vacuum 1.8m deep (17.3psia) @08:28:20.37 Cartridge 8 sampling 269/1000ml at 0.32ml/s with 14.3psi vacuum @08:29:20.37 Cartridge 8 sampling 288/1000ml at 0.35ml/s with 14.5psi vacuum @08:30:20.37 Cartridge 8 sampling 308/1000ml at 0.32ml/s with 14.7psi vacuum @08:31:20.37 Cartridge 8 sampling 328/1000ml at 0.32ml/s with 11.6psi vacuum 1.9m deep (17.5psia) @08:32:20.37 Cartridge 8 sampling 347/1000ml at 0.33ml/s with 11.4psi vacuum 1.6m deep (17.0psia) @08:33:20.37 Cartridge 8 sampling 367/1000ml at 0.32ml/s with 13.4psi vacuum @08:34:20.37 Cartridge 8 sampling 386/1000ml at 0.31ml/s with 13.2psi vacuum @08:35:20.37 Cartridge 8 sampling 405/1000ml at 0.30ml/s with 13.5psi vacuum @08:36:20.37 Cartridge 8 sampling 424/1000ml at 0.33ml/s with 13.5psi vacuum 1.6m deep (17.1psia) @08:37:20.37 Cartridge 8 sampling 443/1000ml at 0.31ml/s with 14.8psi vacuum @08:38:20.37 Cartridge 8 sampling 462/1000ml at 0.34ml/s with 13.3psi vacuum 1.8m deep (17.4psia) @08:39:20.37 Cartridge 8 sampling 480/1000ml at 0.30ml/s with 12.6psi vacuum @08:40:20.37 Cartridge 8 sampling 499/1000ml at 0.31ml/s with 12.9psi vacuum @08:41:20.37 Cartridge 8 sampling 517/1000ml at 0.30ml/s with 14.7psi vacuum @08:42:20.37 Cartridge 8 sampling 535/1000ml at 0.29ml/s with 13.7psi vacuum @08:43:20.37 Cartridge 8 sampling 554/1000ml at 0.30ml/s with 15.3psi vacuum @08:44:20.37 Cartridge 8 sampling 571/1000ml at 0.30ml/s with 13.0psi vacuum @08:45:20.37 Cartridge 8 sampling 589/1000ml at 0.29ml/s with 15.9psi vacuum @08:46:20.75 Cartridge 8 sampling 607/1000ml at 0.28ml/s with 12.7psi vacuum @08:47:20.37 Cartridge 8 sampling 624/1000ml at 0.29ml/s with 11.7psi vacuum @08:48:20.37 Cartridge 8 sampling 641/1000ml at 0.30ml/s with 12.5psi vacuum 1.7m deep (17.3psia) @08:49:20.37 Cartridge 8 sampling 659/1000ml at 0.27ml/s with 11.9psi vacuum @08:50:20.37 Cartridge 8 sampling 676/1000ml at 0.28ml/s with 12.0psi vacuum @08:51:20.37 Cartridge 8 sampling 692/1000ml at 0.26ml/s with 12.5psi vacuum @08:52:20.37 Cartridge 8 sampling 709/1000ml at 0.28ml/s with 11.8psi vacuum @08:53:20.37 Cartridge 8 sampling 726/1000ml at 0.28ml/s with 10.9psi vacuum 2.0m deep (17.6psia) @08:54:20.37 Cartridge 8 sampling 742/1000ml at 0.26ml/s with 12.6psi vacuum @08:55:20.37 Cartridge 8 sampling 759/1000ml at 0.28ml/s with 13.1psi vacuum @08:56:20.37 Cartridge 8 sampling 775/1000ml at 0.28ml/s with 12.3psi vacuum @08:57:20.37 Cartridge 8 sampling 791/1000ml at 0.27ml/s with 11.1psi vacuum 1.8m deep (17.4psia) @08:58:20.37 Cartridge 8 sampling 807/1000ml at 0.25ml/s with 12.8psi vacuum @08:59:20.37 Cartridge 8 sampling 823/1000ml at 0.28ml/s with 14.0psi vacuum 1.6m deep (17.1psia) @09:00:20.37 Cartridge 8 sampling 840/1000ml at 0.28ml/s with 12.9psi vacuum @09:01:20.37 Cartridge 8 sampling 856/1000ml at 0.25ml/s with 13.5psi vacuum @09:02:20.36 Cartridge 8 sampling 872/1000ml at 0.28ml/s with 13.3psi vacuum @09:03:20.37 Cartridge 8 sampling 888/1000ml at 0.26ml/s with 12.6psi vacuum @09:04:20.37 Cartridge 8 sampling 904/1000ml at 0.28ml/s with 13.0psi vacuum 1.7m deep (17.2psia) @09:05:20.37 Cartridge 8 sampling 920/1000ml at 0.27ml/s with 13.5psi vacuum @09:06:20.37 Cartridge 8 sampling 936/1000ml at 0.27ml/s with 14.2psi vacuum @09:07:20.36 Cartridge 8 sampling 951/1000ml at 0.27ml/s with 13.7psi vacuum @09:08:20.37 Cartridge 8 sampling 967/1000ml at 0.26ml/s with 13.7psi vacuum @09:09:20.37 Cartridge 8 sampling 982/1000ml at 0.27ml/s with 11.6psi vacuum @09:10:20.37 Cartridge 8 sampling 998/1000ml at 0.25ml/s with 13.3psi vacuum @09:10:29.89 Pressure.coast @09:10:30.01 Intake.close @09:10:30.05 Exhaust.close @09:10:30.22 Toroid.reconfigure :hold @09:10:30.28 Toroid.seek :bypass @09:10:31.90 Toroid.reconfigure :default @09:10:31.96 Toroid.seek :clear @09:10:33.59 Sampled 1000.0ml @09:10:34.92 -> Cmd.startProcessing @09:10:36.78 SPR.command \"read_sequences\" @09:10:36.94 SPR.command \"stats_on\" @09:10:36.98 SPR.command \"start 0 1\" @09:10:38.98 Begun log /var/log/esp/spr19jul2809h.spr @09:10:40.01 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @09:10:41.68 Handoff.select :waste @09:10:41.85 Archive.seek :empty @09:10:49.03 Syringe tare is 21% of full scale @09:10:59.57 PinPort.seek :cartridge @09:11:04.13 Lysis1.seek 1350ul @09:11:06.60 Lysis2.seek 1700ul @09:11:09.70 Plunge.seek :reagent @09:11:10.53 Evacuating puck with filter bubble point of 30psi @09:11:10.54 PinPort.seek :cartridge @09:11:10.62 Handoff.select :waste @09:11:17.95 Air pumping 1 strokes to reach 4psi @09:11:17.96 Air.reconfigure :gulp @09:11:18.04 Air.seek :tVent @09:11:19.85 0.1psi after gulp @09:11:19.86 Air stroke #1 of 1 @09:11:19.88 Air.reconfigure :pressurize @09:11:19.97 Air.seek :pressurized @09:11:32.81 -0.0psi after pressurize @09:11:32.82 Air.reconfigure :compress @09:11:32.89 Air.seek :evac @09:11:35.56 0.5psi after compress @09:11:49.80 0.3psi after compress (stable) @09:11:49.93 at 0.2psi Opened to waste @09:11:49.94 Handoff.select :blocked @09:11:50.10 Air pumping 10 strokes to reach 30psi @09:11:50.12 Air.reconfigure :gulp @09:11:50.20 Air.seek :tVent @09:12:00.11 9.8psi after gulp @09:12:00.12 Air stroke #1 of 10 @09:12:00.14 Air.reconfigure :pressurize @09:12:00.22 Air.seek :pressurized @09:12:13.06 10.6psi after pressurize @09:12:13.07 Air.reconfigure :compress @09:12:13.15 Air.seek :evac @09:12:15.84 13.0psi after compress @09:12:39.16 27.9psi after compress (stable) @09:12:39.18 Air.reconfigure :gulp @09:12:39.25 Air.seek :tVent @09:12:49.15 28.4psi after gulp @09:12:49.16 Air stroke #2 of 10 @09:12:49.18 Air.reconfigure :pressurize @09:12:49.26 Air.seek :pressurized @09:13:02.11 26.9psi after pressurize @09:13:02.14 Air.reconfigure :compress @09:13:02.22 Air.seek :evac @09:13:04.89 28.8psi after compress @09:13:05.24 Reached 30.4 of 30.0psi after pumping 4672ul (3.00 strokes) @09:13:05.25 Handoff.select :waste @09:13:28.64 delay 1:00 @09:14:28.66 Handoff.select :blocked @09:14:53.70 Handoff.select :waste @09:14:53.83 PSI at @1.33psi at evacEnd @09:14:53.84 Handoff.select :blocked @09:14:53.96 Air.reconfigure :gulp @09:14:54.04 Air.seek :tVent @09:15:11.11 Stable @1.1psi after Handoff blocked @09:15:11.19 Air pumping 10 strokes to reach 26psi @09:15:11.20 Air stroke #1 of 10 @09:15:11.22 Air.reconfigure :pressurize @09:15:11.30 Air.seek :pressurized @09:15:24.14 1.4psi after pressurize @09:15:24.15 Air.reconfigure :compress @09:15:24.24 Air.seek :evac @09:15:26.91 2.1psi after compress @09:15:55.19 11.7psi after compress (stable) @09:15:55.22 Air.reconfigure :gulp @09:15:55.30 Air.seek :tVent @09:16:05.20 12.3psi after gulp @09:16:05.21 Air stroke #2 of 10 @09:16:05.23 Air.reconfigure :pressurize @09:16:05.31 Air.seek :pressurized @09:16:18.15 12.7psi after pressurize @09:16:18.17 Air.reconfigure :compress @09:16:18.24 Air.seek :evac @09:16:20.91 13.4psi after compress @09:16:42.60 22.2psi after compress (stable) @09:16:42.62 Air.reconfigure :gulp @09:16:42.70 Air.seek :tVent @09:16:52.59 23.0psi after gulp @09:16:52.60 Air stroke #3 of 10 @09:16:52.62 Air.reconfigure :pressurize @09:16:52.70 Air.seek :pressurized @09:17:05.54 23.1psi after pressurize @09:17:05.55 Air.reconfigure :compress @09:17:05.64 Air.seek :evac @09:17:08.30 23.6psi after compress @09:17:10.01 Reached 26.3 of 26.1psi after pumping 6230ul (4.00 strokes) @09:17:10.02 Handoff.select :waste @09:17:17.36 delay 1:00 @09:18:17.39 Handoff.select :blocked @09:18:42.72 Handoff.select :waste @09:18:42.84 PSI at @0.95psi at evacEnd @09:18:42.85 Air.reconfigure :default @09:18:42.95 Air.seek :fVent @09:18:44.39 delay 5 seconds @09:18:49.41 Seawater evacuated, Presure above/below filter released. Lysis begins @09:18:49.48 Lysis1.reconfigure :slow @09:18:49.55 Lysis1.seek 1050ul @09:19:19.59 delay 5 seconds @09:19:24.61 Handoff.select :blocked @09:19:24.77 Lysis1.seek 450ul @09:20:24.76 delay 3 seconds @09:20:27.84 Air.reconfigure to mix into 1.37806 parts Lysis1 @09:20:28.26 Air.seek :evac @09:20:28.30 Lysis1.seek 300ul @09:20:43.41 Air.reconfigure :default @09:20:43.47 Air.seek :full @09:20:57.69 Lysis1.seek :empty @09:21:27.81 Lysis Delivered. Syringe pressure @45.9psi @09:21:27.85 PuckHeater.seek 85.7C @09:22:21.56 PuckHeater.hold 15:00,85.7C @09:37:20.85 Lysis complete. Syringe pressure @58.1psi @09:37:20.86 Handoff.select :blocked @09:37:20.96 Lysis1.reconfigure :halfSpeed @09:37:21.03 Lysis1.seek 200ul @09:37:24.81 delay 1 second @09:37:25.83 Lysis1.seek :empty @09:37:29.65 Initial AirS pressure @59.4psi @09:37:29.71 Lysis1.reconfigure :extract @09:37:29.81 Lysis1.seek 875ul,10:00 @09:37:31.50 Air pumping 10 strokes to reach 21psi @09:37:31.51 Air stroke #1 of 10 @09:37:31.53 Air.reconfigure :pressurize @09:37:31.61 Air.seek :pressurized @09:37:44.45 15.7psi after pressurize @09:37:44.46 Air.reconfigure :compress @09:37:44.53 Air.seek :evac @09:37:47.20 17.1psi after compress @09:38:01.46 15.9psi after compress (stable) @09:38:01.48 Air.reconfigure :gulp @09:38:01.55 Air.seek :tVent @09:38:11.45 14.3psi after gulp @09:38:11.46 Air stroke #2 of 10 @09:38:11.48 Air.reconfigure :pressurize @09:38:11.56 Air.seek :pressurized @09:38:24.39 15.4psi after pressurize @09:38:24.40 Air.reconfigure :compress @09:38:24.48 Air.seek :evac @09:38:27.15 16.8psi after compress @09:38:32.39 Air.reconfigure :default @09:38:32.46 Air.hold @09:38:32.66 Lysis1 at 875ul pressure @17.3psi @09:38:32.67 Lysis1.reconfigure :default @09:38:32.75 Lysis1.seek 900ul @09:38:33.54 Lysis1 at 900ul pressure(finPSI) @14.3psi @09:38:33.56 delay 1 second @09:38:34.67 Lysis1 at 900ul pressure @16.4psi @09:38:34.69 Air.reconfigure :halfSpeed @09:38:34.77 Air.seek :fVent @09:38:37.01 Puck Top Vented. Syringe pressure @4.9psi @09:38:37.02 Handoff.select :waste @09:38:37.14 delay 5 seconds @09:38:42.16 Lysate Recovered in LyS1 @09:38:42.17 Lysis1.reconfigure :DAmix @09:38:42.28 Lysis2.reconfigure :DAmix @09:38:42.69 Lysis1.seek 650ul @09:38:42.76 Lysis2.seek 1450ul @09:39:17.79 delay 5 seconds @09:39:22.81 Handoff.select SPR @09:39:22.92 SPR.command \"start 2 14\" @09:39:24.60 delay 10 seconds @09:39:34.63 SPR.command \"start 1 0\" @09:39:37.39 Waiting up to 4:00 for SPR to enter state 1 @09:42:10.02 SPR.command \"hold_on\" @09:42:11.45 SPR Dilution 1 of 1 -- Running Experiment 1 @09:42:11.86 Lysis1.seek 350ul @09:42:11.91 Lysis2.seek 1150ul @09:42:53.92 volL1 = 300.0 and volL2 = 300.0 @09:42:53.93 delay 15 seconds @09:43:08.96 SPR.command \"hold_off\" @09:43:12.10 Waiting up to 25:00 for SPR to enter state 7 @10:03:25.94 SPR Analysis has Completed @10:03:25.96 Handoff.select :waste @10:03:26.88 Ended log /var/log/esp/spr19jul2809h.spr @10:03:26.90 SPR.command \"stats_off\" @10:03:28.38 -> Cmd.stop @10:03:28.47 Handoff.select :close @10:03:28.60 Gate.power :heat,:OFF @10:03:28.62 Gate.power :cartridge,:OFF @10:03:28.69 Carousel.seek 8 @10:03:28.87 Plunge.seek :locked @10:03:29.70 PinPort.seek :clear @10:03:34.31 Archive.seek :clear @10:03:34.44 Air.reconfigure :default @10:03:34.57 Lysis1.reconfigure :default @10:03:34.68 Air.seek :clear @10:03:34.74 Lysis1.seek :clear @10:03:34.82 Lysis2.reconfigure :default @10:03:34.90 Lysis2.seek :clear @10:03:52.07 Archive.coast @10:03:52.12 Air.coast @10:03:52.17 Lysis1.coast @10:03:52.22 Lysis2.coast @10:03:52.27 Plunge.coast @10:03:52.32 SamplePump.coast @10:03:52.37 Toroid.coast @10:03:52.42 Carousel.coast @10:03:52.81 PinPort.coast @10:03:53.01 Gate.power :motor,:OFF @10:03:53.03 Gate.power :core,:OFF @10:03:53.05 Gate.power :analytic1,:OFF @10:03:53.07 Gate.power :analytic2,:OFF @10:03:53.09 Gate.power :analytic3,:OFF @10:03:53.12 Gate.power :main,:OFF @10:03:53.14 Safely stopped and ready to power off ##-- file closed 2019-07-28T21:19:38.521Z ##-- file opened 2019-07-28T21:19:38.523Z @14:19:39.01 -> showStatus @14:19:41.02 -> Cmd.loadCartridge -9 @14:19:41.15 Gate.power :main,:ON,limit to 5000mA @14:19:41.16 Gate.power :analytic1,:ON,limit to 5000mA @14:19:41.18 Gate.power :analytic2,:ON,limit to 5000mA @14:19:41.21 Gate.power :analytic3,:ON,limit to 5000mA @14:19:41.34 Powering core bus @14:19:43.62 SPR Module 1, FW Rev. B, FPGA Rev. C @14:19:43.75 Archive.configure :default @14:19:43.91 Air.configure :default @14:19:44.01 Lysis1.configure :default @14:19:44.11 Lysis2.configure :default @14:19:44.23 Plunge.configure :default @14:19:44.35 SamplePump.configure :default @14:19:44.47 ExtraPump.configure :default @14:19:44.59 Toroid.configure :default @14:19:44.71 Carousel.configure :default @14:19:44.83 Pressure.configure :default @14:19:44.93 PinPort.configure :default @14:19:44.99 All elf controllers running firmware version 4.58 @14:19:45.02 Gate.power :motor,:ON,limit to 5000mA @14:19:45.21 Archive.reconfigure :default @14:19:45.44 Archive.home.jog 0 @14:19:45.57 Toroid.reconfigure :default @14:19:45.65 Plunge.reconfigure :default @14:19:45.68 Lysis2.reconfigure :default @14:19:45.72 Lysis1.reconfigure :default @14:19:45.77 PinPort.reconfigure :default @14:19:45.83 Air.reconfigure :default @14:19:46.05 Lysis2.home.jog 0 @14:19:46.10 Toroid.home.jog 0 @14:19:46.16 Lysis1.home.jog 0 @14:19:46.22 Plunge.home.jog 0 @14:19:46.28 Air.home.jog 0 @14:19:46.34 PinPort.home.jog 0 @14:19:46.51 Archive.reconfigure :homing @14:19:46.84 Archive.jog 1408 @14:19:47.00 Toroid.reconfigure :homing @14:19:47.03 Plunge.reconfigure :homing @14:19:47.06 Air.reconfigure :homing @14:19:47.10 PinPort.reconfigure :homing @14:19:47.14 Lysis2.reconfigure :homing @14:19:47.18 Lysis1.reconfigure :homing @14:19:47.40 Lysis1.jog 1406 @14:19:47.45 Air.jog 1412 @14:19:47.51 Plunge.jog 1557 @14:19:47.56 PinPort.jog -106 @14:19:47.62 Toroid.jog 630 @14:19:47.68 Lysis2.jog 162 @14:19:47.79 PinPort.setRawPosition! 0 @14:19:47.83 PinPort.seek :home @14:19:47.92 PinPort.reconfigure :default @14:19:48.00 Archive.setRawPosition! 50 @14:19:48.05 Archive.seek :home @14:19:48.14 Lysis2.setRawPosition! 50 @14:19:48.18 Lysis2.seek :home @14:19:48.34 Archive.reconfigure :default @14:19:48.46 Lysis2.reconfigure :default @14:19:48.55 Lysis1.setRawPosition! 50 @14:19:48.60 Lysis1.seek :home @14:19:48.69 Air.setRawPosition! 50 @14:19:48.75 Air.seek :home @14:19:48.83 Plunge.setRawPosition! 50 @14:19:48.89 Plunge.seek :home @14:19:48.97 Lysis1.reconfigure :default @14:19:49.06 Air.reconfigure :default @14:19:49.17 Plunge.reconfigure :default @14:19:49.75 Toroid.setRawPosition! 0 @14:19:49.79 Toroid.seek :home @14:19:49.88 Toroid.reconfigure :default @14:19:50.00 Toroid.seek :clear @14:19:51.63 Carousel.reconfigure :default @14:19:51.70 Carousel.home.jog 0 @14:19:52.19 Carousel.seek 8 @14:19:52.33 Selecting Cartridge 7 @14:19:52.58 Carousel.seek 7 @14:19:53.06 Lysis1.seek :locked @14:19:57.19 Plunge.seek :locked @14:19:57.31 Air.seek :locked @14:20:01.44 Powering cartridge bus @14:20:03.57 Gate.setIR 1750,5000,0,6 @14:20:03.69 Gate.setIR 0,5000,0,6 @14:20:03.75 Loaded Lyse-n-Go Cartridge @14:20:03.76 PuckHeater.configure HeatConfig @14:20:03.82 Gate.power :heat,:ON,limit to 5000mA @14:20:19.98 -> Cmd.startFiltering @14:20:20.21 Duration of filtering limited to 2:05:00 @14:20:20.47 Priming sample loop w/100ml, bypass w/10ml @14:20:20.48 SamplePump.setPosition! 0ml @14:20:20.52 Bypass.open @14:20:22.01 Intake.open @14:20:22.83 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:20:26.03 Vacuum tare is 52% of full scale @14:20:26.15 Pressure.reconfigure :default @14:20:26.21 Pressure.hold @14:20:26.38 SamplePump.reconfigure :prime @14:20:26.46 SamplePump.seek 10ml,1:10 @14:20:35.41 Pressure.coast @14:20:35.46 SamplePump.setPosition! 0ml @14:20:35.51 Exhaust.open @14:20:36.90 Bypass.close @14:20:38.40 Pressure.reconfigure :default @14:20:38.46 Pressure.hold @14:20:38.68 SamplePump.reconfigure :prime @14:20:38.76 SamplePump.seek 100ml,7:10 @14:22:34.38 Pressure.coast @14:22:34.44 Bypass.open @14:22:35.13 Intake.close @14:22:35.96 Toroid.reconfigure :hold @14:22:36.02 Toroid.seek :crack @14:22:36.82 Toroid.reconfigure :default @14:22:36.88 Toroid.seek :clear @14:22:38.44 Toroid.reconfigure :hold @14:22:38.51 Toroid.seek :cartridge @14:22:40.32 Toroid.reconfigure :default @14:22:40.38 Toroid.seek :clear @14:22:42.34 Pressurizing cartridge to 14psi above 21.0psia @14:22:42.35 SamplePump.setPosition! 0ml @14:22:42.40 Intake.open @14:22:43.14 Bypass.close @14:22:43.83 Exhaust.close @14:22:44.62 Pressure.reconfigure :default @14:22:44.68 Pressure.hold @14:22:44.84 SamplePump.reconfigure :charge @14:22:44.91 SamplePump.seek 2.3ml,41 seconds @14:22:51.84 Pressure.coast @14:22:51.90 Intake.close @14:22:52.69 Pressurized cartridge with 0.223ml to 14.0psi @14:22:52.70 Isolated @33.2psia (12.2psi) @14:22:52.71 Checking for cartridge leaks from 33.2psia @14:22:52.74 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @14:22:57.84 Sampling 1000.0ml @14:22:57.86 SamplePump.setPosition! 0ml @14:22:57.91 Exhaust.open @14:22:58.61 Intake.open @14:22:59.47 Pressure.reconfigure :default @14:22:59.53 Pressure.hold @14:22:59.64 Sea 4.4m deep (21.3psia) @14:22:59.77 SamplePump.reconfigure :sample @14:22:59.84 SamplePump.seek 1000ml,1:07:09 @14:23:02.51 Cartridge 7 sampling 1/1000ml at 0.34ml/s with 13.6psi vacuum 3.1m deep (19.3psia) @14:24:02.51 Cartridge 7 sampling 21/1000ml at 0.33ml/s with 13.8psi vacuum @14:25:02.51 Cartridge 7 sampling 42/1000ml at 0.36ml/s with 13.3psi vacuum 2.1m deep (17.8psia) @14:26:02.51 Cartridge 7 sampling 61/1000ml at 0.32ml/s with 14.0psi vacuum @14:27:02.51 Cartridge 7 sampling 81/1000ml at 0.32ml/s with 12.5psi vacuum @14:28:02.51 Cartridge 7 sampling 100/1000ml at 0.33ml/s with 13.5psi vacuum @14:29:02.51 Cartridge 7 sampling 119/1000ml at 0.31ml/s with 12.8psi vacuum @14:30:02.51 Cartridge 7 sampling 137/1000ml at 0.31ml/s with 12.7psi vacuum @14:31:02.51 Cartridge 7 sampling 155/1000ml at 0.28ml/s with 12.9psi vacuum @14:32:02.51 Cartridge 7 sampling 172/1000ml at 0.28ml/s with 12.4psi vacuum @14:33:02.51 Cartridge 7 sampling 189/1000ml at 0.26ml/s with 13.9psi vacuum @14:34:02.51 Cartridge 7 sampling 205/1000ml at 0.26ml/s with 14.4psi vacuum 1.8m deep (17.4psia) @14:35:02.51 Cartridge 7 sampling 221/1000ml at 0.26ml/s with 13.4psi vacuum @14:36:02.51 Cartridge 7 sampling 236/1000ml at 0.25ml/s with 13.8psi vacuum @14:37:02.51 Cartridge 7 sampling 251/1000ml at 0.25ml/s with 13.8psi vacuum @14:37:12.34 Flow averaged 0.30ml/s over 14:03 @14:37:12.37 Waiting up to 7 seconds for vacuum to reduce from 14.6 to 10.0psi @14:37:13.37 Relieved excess vacuum in 10.8 seconds @14:37:13.39 SamplePump.seek 1000ml,50:15 @14:37:15.40 Flow estimate reduced to 0.24ml/s @14:37:15.42 Intake.close @14:37:15.47 Exhaust.close @14:37:15.64 Toroid.reconfigure :hold @14:37:15.71 Toroid.seek :bypass @14:37:17.34 Toroid.reconfigure :default @14:37:17.41 Toroid.seek :clear @14:37:19.03 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @14:37:19.05 Pressure.coast @14:37:19.23 Sampled 254.2ml @14:37:21.43 -> Cmd.startProcessing @14:37:23.64 SPR.command \"read_sequences\" @14:37:23.79 SPR.command \"stats_on\" @14:37:23.83 SPR.command \"start 0 1\" @14:37:25.84 Begun log /var/log/esp/spr19jul2814h.spr @14:37:26.64 Handoff.select :waste @14:37:26.82 Archive.seek :empty @14:37:34.05 Syringe tare is 21% of full scale @14:37:44.55 PinPort.seek :cartridge @14:37:49.11 Lysis1.seek 1350ul @14:37:51.58 Lysis2.seek 1700ul @14:37:54.68 Plunge.seek :reagent @14:37:55.52 Evacuating puck with filter bubble point of 30psi @14:37:55.53 PinPort.seek :cartridge @14:37:55.61 Handoff.select :waste @14:38:02.93 Air pumping 1 strokes to reach 4psi @14:38:02.95 Air.reconfigure :gulp @14:38:03.04 Air.seek :tVent @14:38:04.83 0.0psi after gulp @14:38:04.84 Air stroke #1 of 1 @14:38:04.86 Air.reconfigure :pressurize @14:38:04.95 Air.seek :pressurized @14:38:17.79 0.0psi after pressurize @14:38:17.80 Air.reconfigure :compress @14:38:17.88 Air.seek :evac @14:38:20.54 0.1psi after compress @14:38:34.44 0.0psi after compress (stable) @14:38:34.56 at 0.0psi Opened to waste @14:38:34.57 Handoff.select :blocked @14:38:34.74 Air pumping 10 strokes to reach 30psi @14:38:34.76 Air.reconfigure :gulp @14:38:34.84 Air.seek :tVent @14:38:44.73 1.8psi after gulp @14:38:44.74 Air stroke #1 of 10 @14:38:44.76 Air.reconfigure :pressurize @14:38:44.84 Air.seek :pressurized @14:38:57.68 3.3psi after pressurize @14:38:57.69 Air.reconfigure :compress @14:38:57.77 Air.seek :evac @14:39:00.44 3.8psi after compress @14:40:05.61 24.1psi after compress (stable) @14:40:05.63 Air.reconfigure :gulp @14:40:05.70 Air.seek :tVent @14:40:15.60 25.7psi after gulp @14:40:15.61 Air stroke #2 of 10 @14:40:15.62 Air.reconfigure :pressurize @14:40:15.71 Air.seek :pressurized @14:40:28.54 25.4psi after pressurize @14:40:28.55 Air.reconfigure :compress @14:40:28.63 Air.seek :evac @14:40:31.30 25.1psi after compress @14:40:45.28 Reached 30.0 of 30.0psi after pumping 4672ul (3.00 strokes) @14:40:45.29 Handoff.select :waste @14:40:57.12 delay 1:00 @14:41:57.14 Handoff.select :blocked @14:42:03.99 Pressure too High, open to waste again (4.6psi) @14:42:04.00 Handoff.select :waste @14:42:04.13 delay 1:00 @14:43:04.15 Handoff.select :blocked @14:43:13.20 Pressure too High, open to waste again (4.2psi) @14:43:13.21 Handoff.select :waste @14:43:13.36 delay 1:00 @14:44:13.38 Handoff.select :blocked @14:44:38.35 Handoff.select :waste @14:44:38.49 PSI at @2.23psi at evacEnd @14:44:38.50 Handoff.select :blocked @14:44:38.62 Air.reconfigure :gulp @14:44:38.71 Air.seek :tVent @14:44:56.16 Stable @1.8psi after Handoff blocked @14:44:56.25 Air pumping 10 strokes to reach 27psi @14:44:56.26 Air stroke #1 of 10 @14:44:56.28 Air.reconfigure :pressurize @14:44:56.37 Air.seek :pressurized @14:45:09.21 2.4psi after pressurize @14:45:09.23 Air.reconfigure :compress @14:45:09.30 Air.seek :evac @14:45:11.97 2.8psi after compress @14:45:29.19 7.5psi after compress (stable) @14:45:29.21 Air.reconfigure :gulp @14:45:29.29 Air.seek :tVent @14:45:39.18 7.6psi after gulp @14:45:39.19 Air stroke #2 of 10 @14:45:39.21 Air.reconfigure :pressurize @14:45:39.29 Air.seek :pressurized @14:45:52.13 7.6psi after pressurize @14:45:52.17 Air.reconfigure :compress @14:45:52.25 Air.seek :evac @14:45:54.91 7.6psi after compress @14:46:08.83 8.2psi after compress (stable) @14:46:08.85 Air.reconfigure :gulp @14:46:08.93 Air.seek :tVent @14:46:18.82 19.2psi after gulp @14:46:18.83 Air stroke #3 of 10 @14:46:18.85 Air.reconfigure :pressurize @14:46:18.93 Air.seek :pressurized @14:46:31.77 21.0psi after pressurize @14:46:31.78 Air.reconfigure :compress @14:46:31.87 Air.seek :evac @14:46:34.55 21.5psi after compress @14:46:42.23 Reached 26.8 of 26.8psi after pumping 6230ul (4.00 strokes) @14:46:42.25 Handoff.select :waste @14:46:50.52 delay 1:00 @14:47:50.54 Handoff.select :blocked @14:48:15.56 Handoff.select :waste @14:48:15.68 PSI at @2.83psi at evacEnd @14:48:15.70 Air.reconfigure :default @14:48:15.78 Air.seek :fVent @14:48:17.22 delay 5 seconds @14:48:22.24 Seawater evacuated, Presure above/below filter released. Lysis begins @14:48:22.32 Lysis1.reconfigure :slow @14:48:22.38 Lysis1.seek 1050ul @14:48:52.42 delay 5 seconds @14:48:57.44 Handoff.select :blocked @14:48:57.61 Lysis1.seek 450ul @14:49:57.58 delay 3 seconds @14:50:00.65 Air.reconfigure to mix into 1.37806 parts Lysis1 @14:50:01.13 Air.seek :evac @14:50:01.17 Lysis1.seek 300ul @14:50:16.29 Air.reconfigure :default @14:50:16.35 Air.seek :full @14:50:30.58 Lysis1.seek :empty @14:51:00.67 Lysis Delivered. Syringe pressure @40.4psi @14:51:00.71 PuckHeater.seek 85.7C @14:52:03.93 PuckHeater.hold 15:00,85.7C @15:07:01.30 Lysis complete. Syringe pressure @49.0psi @15:07:01.31 Handoff.select :blocked @15:07:01.41 Lysis1.reconfigure :halfSpeed @15:07:01.49 Lysis1.seek 200ul @15:07:05.26 delay 1 second @15:07:06.29 Lysis1.seek :empty @15:07:10.11 Initial AirS pressure @51.5psi @15:07:10.17 Lysis1.reconfigure :extract @15:07:10.27 Lysis1.seek 875ul,10:00 @15:07:11.41 Air pumping 10 strokes to reach 21psi @15:07:11.42 Air stroke #1 of 10 @15:07:11.44 Air.reconfigure :pressurize @15:07:11.52 Air.seek :pressurized @15:07:24.36 15.8psi after pressurize @15:07:24.37 Air.reconfigure :compress @15:07:24.44 Air.seek :evac @15:07:27.45 16.1psi after compress @15:07:41.41 15.7psi after compress (stable) @15:07:41.43 Air.reconfigure :gulp @15:07:41.50 Air.seek :tVent @15:07:51.40 16.3psi after gulp @15:07:51.41 Air stroke #2 of 10 @15:07:51.43 Air.reconfigure :pressurize @15:07:51.51 Air.seek :pressurized @15:08:04.34 15.7psi after pressurize @15:08:04.35 Air.reconfigure :compress @15:08:04.42 Air.seek :evac @15:08:07.09 16.8psi after compress @15:08:21.01 15.9psi after compress (stable) @15:08:21.03 Air.reconfigure :gulp @15:08:21.10 Air.seek :tVent @15:08:30.99 16.3psi after gulp @15:08:31.00 Air stroke #3 of 10 @15:08:31.03 Air.reconfigure :pressurize @15:08:31.10 Air.seek :pressurized @15:08:43.94 15.8psi after pressurize @15:08:43.95 Air.reconfigure :compress @15:08:44.03 Air.seek :evac @15:08:46.69 16.2psi after compress @15:09:00.61 15.8psi after compress (stable) @15:09:00.64 Air.reconfigure :gulp @15:09:00.72 Air.seek :tVent @15:09:10.61 16.0psi after gulp @15:09:10.62 Air stroke #4 of 10 @15:09:10.64 Air.reconfigure :pressurize @15:09:10.71 Air.seek :pressurized @15:09:23.55 15.8psi after pressurize @15:09:23.56 Air.reconfigure :compress @15:09:23.64 Air.seek :evac @15:09:26.30 15.7psi after compress @15:09:29.23 Air.reconfigure :default @15:09:29.29 Air.hold @15:09:29.46 Lysis1 at 875ul pressure @15.6psi @15:09:29.47 Lysis1.reconfigure :default @15:09:29.55 Lysis1.seek 900ul @15:09:30.32 Lysis1 at 900ul pressure(finPSI) @10.0psi @15:09:30.33 delay 1 second @15:09:31.83 Lysis1 at 900ul pressure @12.2psi @15:09:31.85 Air.reconfigure :halfSpeed @15:09:31.93 Air.seek :fVent @15:09:34.18 Puck Top Vented. Syringe pressure @3.9psi @15:09:34.19 Handoff.select :waste @15:09:34.31 delay 5 seconds @15:09:39.33 Lysate Recovered in LyS1 @15:09:39.34 Lysis2.reconfigure :DAmix @15:09:39.47 Lysis1.reconfigure :DAmix @15:09:39.81 Lysis2.seek 1450ul @15:09:39.85 Lysis1.seek 650ul @15:10:14.92 delay 5 seconds @15:10:19.94 Handoff.select SPR @15:10:20.07 SPR.command \"start 2 14\" @15:10:21.64 delay 10 seconds @15:10:31.66 SPR.command \"start 1 0\" @15:10:35.36 Waiting up to 4:00 for SPR to enter state 1 @15:13:08.46 SPR.command \"hold_on\" @15:13:09.90 SPR Dilution 1 of 1 -- Running Experiment 1 @15:13:10.32 Lysis2.seek 1150ul @15:13:10.36 Lysis1.seek 350ul @15:13:52.39 volL1 = 300.0 and volL2 = 300.0 @15:13:52.40 delay 15 seconds @15:14:07.43 SPR.command \"hold_off\" @15:14:10.54 Waiting up to 25:00 for SPR to enter state 7 @15:34:24.38 SPR Analysis has Completed @15:34:24.40 Handoff.select :waste @15:34:25.32 Ended log /var/log/esp/spr19jul2814h.spr @15:34:25.33 SPR.command \"stats_off\" @15:34:26.79 -> Cmd.stop @15:34:26.88 Handoff.select :close @15:34:27.01 Gate.power :heat,:OFF @15:34:27.03 Gate.power :cartridge,:OFF @15:34:27.11 Carousel.seek 7 @15:34:27.29 Plunge.seek :locked @15:34:28.12 PinPort.seek :clear @15:34:32.74 Archive.seek :clear @15:34:32.87 Air.reconfigure :default @15:34:33.01 Lysis1.reconfigure :default @15:34:33.11 Air.seek :clear @15:34:33.16 Lysis1.seek :clear @15:34:33.24 Lysis2.reconfigure :default @15:34:33.31 Lysis2.seek :clear @15:34:50.50 Archive.coast @15:34:50.56 Air.coast @15:34:50.60 Lysis1.coast @15:34:50.65 Lysis2.coast @15:34:50.70 Plunge.coast @15:34:50.76 SamplePump.coast @15:34:51.16 Toroid.coast @15:34:51.21 Carousel.coast @15:34:51.26 PinPort.coast @15:34:51.45 Gate.power :motor,:OFF @15:34:51.47 Gate.power :core,:OFF @15:34:51.49 Gate.power :analytic1,:OFF @15:34:51.51 Gate.power :analytic2,:OFF @15:34:51.53 Gate.power :analytic3,:OFF @15:34:51.54 Gate.power :main,:OFF @15:34:51.57 Safely stopped and ready to power off ##-- file closed 2019-07-28T23:20:52.674Z ##-- file opened 2019-07-28T23:20:52.677Z @16:20:52.96 -> showStatus @16:20:55.09 -> Cmd.loadCartridge -9 @16:20:55.22 Gate.power :main,:ON,limit to 5000mA @16:20:55.23 Gate.power :analytic1,:ON,limit to 5000mA @16:20:55.26 Gate.power :analytic2,:ON,limit to 5000mA @16:20:55.28 Gate.power :analytic3,:ON,limit to 5000mA @16:20:55.41 Powering core bus @16:20:57.70 SPR Module 1, FW Rev. B, FPGA Rev. C @16:20:57.82 Archive.configure :default @16:20:57.94 Air.configure :default @16:20:58.07 Lysis1.configure :default @16:20:58.17 Lysis2.configure :default @16:20:58.29 Plunge.configure :default @16:20:58.41 SamplePump.configure :default @16:20:58.53 ExtraPump.configure :default @16:20:58.65 Toroid.configure :default @16:20:58.77 Carousel.configure :default @16:20:58.89 Pressure.configure :default @16:20:58.99 PinPort.configure :default @16:20:59.05 All elf controllers running firmware version 4.58 @16:20:59.07 Gate.power :motor,:ON,limit to 5000mA @16:20:59.19 Archive.reconfigure :default @16:20:59.52 Archive.home.jog 0 @16:20:59.67 Toroid.reconfigure :default @16:20:59.74 Plunge.reconfigure :default @16:20:59.77 Lysis2.reconfigure :default @16:20:59.81 Air.reconfigure :default @16:20:59.85 PinPort.reconfigure :default @16:20:59.88 Lysis1.reconfigure :default @16:21:00.16 Air.home.jog 0 @16:21:00.21 PinPort.home.jog 0 @16:21:00.27 Toroid.home.jog 0 @16:21:00.32 Lysis2.home.jog 0 @16:21:00.38 Lysis1.home.jog 0 @16:21:00.44 Plunge.home.jog 0 @16:21:00.71 Archive.reconfigure :homing @16:21:00.97 Toroid.reconfigure :homing @16:21:01.00 Plunge.reconfigure :homing @16:21:01.04 Air.reconfigure :homing @16:21:01.08 Lysis1.reconfigure :homing @16:21:01.12 PinPort.reconfigure :homing @16:21:01.17 Lysis2.reconfigure :homing @16:21:01.38 Lysis2.jog 163 @16:21:01.43 Toroid.jog 627 @16:21:01.49 Plunge.jog 1558 @16:21:01.54 Archive.jog 1407 @16:21:01.60 Lysis1.jog 1406 @16:21:01.66 Air.jog 1416 @16:21:01.72 PinPort.jog -106 @16:21:01.81 Lysis2.setRawPosition! 50 @16:21:01.86 Lysis2.seek :home @16:21:01.95 PinPort.setRawPosition! 0 @16:21:01.99 PinPort.seek :home @16:21:02.07 PinPort.reconfigure :default @16:21:02.16 Lysis2.reconfigure :default @16:21:02.72 Plunge.setRawPosition! 50 @16:21:02.75 Archive.setRawPosition! 50 @16:21:02.83 Plunge.seek :home @16:21:02.88 Archive.seek :home @16:21:03.00 Lysis1.setRawPosition! 50 @16:21:03.02 Air.setRawPosition! 50 @16:21:03.10 Air.seek :home @16:21:03.13 Lysis1.seek :home @16:21:03.31 Plunge.reconfigure :default @16:21:03.33 Archive.reconfigure :default @16:21:03.49 Air.reconfigure :default @16:21:03.58 Toroid.setRawPosition! 0 @16:21:03.60 Lysis1.reconfigure :default @16:21:03.66 Toroid.seek :home @16:21:03.78 Toroid.reconfigure :default @16:21:04.04 Toroid.seek :clear @16:21:05.66 Carousel.reconfigure :default @16:21:05.73 Carousel.home.jog 0 @16:21:06.23 Carousel.seek 7 @16:21:06.38 Selecting Cartridge 6 @16:21:06.63 Carousel.seek 6 @16:21:07.11 Lysis1.seek :locked @16:21:11.24 Plunge.seek :locked @16:21:11.36 Air.seek :locked @16:21:15.49 Powering cartridge bus @16:21:17.63 Gate.setIR 1750,5000,0,6 @16:21:17.75 Gate.setIR 0,5000,0,6 @16:21:17.80 Loaded Lyse-n-Go Cartridge @16:21:17.81 PuckHeater.configure HeatConfig @16:21:17.87 Gate.power :heat,:ON,limit to 5000mA @16:21:34.10 -> Cmd.startFiltering @16:21:34.33 Duration of filtering limited to 2:05:00 @16:21:34.58 Priming sample loop w/100ml, bypass w/10ml @16:21:34.60 SamplePump.setPosition! 0ml @16:21:34.64 Bypass.open @16:21:36.08 Intake.open @16:21:36.92 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:21:40.11 Vacuum tare is 52% of full scale @16:21:40.24 Pressure.reconfigure :default @16:21:40.30 Pressure.hold @16:21:40.47 SamplePump.reconfigure :prime @16:21:40.54 SamplePump.seek 10ml,1:10 @16:21:49.50 Pressure.coast @16:21:49.55 SamplePump.setPosition! 0ml @16:21:49.60 Exhaust.open @16:21:51.04 Bypass.close @16:21:52.56 Pressure.reconfigure :default @16:21:52.62 Pressure.hold @16:21:52.84 SamplePump.reconfigure :prime @16:21:52.91 SamplePump.seek 100ml,7:10 @16:23:49.00 Pressure.coast @16:23:49.06 Bypass.open @16:23:49.77 Intake.close @16:23:50.60 Toroid.reconfigure :hold @16:23:50.66 Toroid.seek :crack @16:23:51.46 Toroid.reconfigure :default @16:23:51.52 Toroid.seek :clear @16:23:53.08 Toroid.reconfigure :hold @16:23:53.15 Toroid.seek :cartridge @16:23:54.95 Toroid.reconfigure :default @16:23:55.01 Toroid.seek :clear @16:23:56.97 Pressurizing cartridge to 14psi above 18.6psia @16:23:56.99 SamplePump.setPosition! 0ml @16:23:57.03 Intake.open @16:23:57.77 Bypass.close @16:23:58.48 Exhaust.close @16:23:59.31 Pressure.reconfigure :default @16:23:59.37 Pressure.hold @16:23:59.53 SamplePump.reconfigure :charge @16:23:59.60 SamplePump.seek 2.3ml,41 seconds @16:24:08.29 Pressure.coast @16:24:08.35 Intake.close @16:24:09.17 Pressurized cartridge with 0.352ml to 14.1psi @16:24:09.18 Isolated @31.2psia (12.6psi) @16:24:09.19 Checking for cartridge leaks from 31.2psia @16:24:09.23 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @16:24:14.26 Cartridge pressure fell 3% to 30.3psia @16:24:14.34 Sampling 1000.0ml @16:24:14.35 SamplePump.setPosition! 0ml @16:24:14.41 Exhaust.open @16:24:15.12 Intake.open @16:24:15.47 Pressure.reconfigure :default @16:24:15.52 Pressure.hold @16:24:15.63 Sea 4.7m deep (21.7psia) @16:24:15.77 SamplePump.reconfigure :sample @16:24:15.84 SamplePump.seek 1000ml,1:07:09 @16:24:18.50 Cartridge 6 sampling 1/1000ml at 0.32ml/s with 12.8psi vacuum 2.1m deep (17.8psia) @16:25:18.51 Cartridge 6 sampling 21/1000ml at 0.32ml/s with 13.3psi vacuum 2.0m deep (17.6psia) @16:26:18.51 Cartridge 6 sampling 42/1000ml at 0.35ml/s with 14.6psi vacuum @16:27:18.51 Cartridge 6 sampling 62/1000ml at 0.33ml/s with 11.5psi vacuum 1.9m deep (17.5psia) @16:28:18.51 Cartridge 6 sampling 81/1000ml at 0.32ml/s with 14.4psi vacuum @16:29:18.51 Cartridge 6 sampling 101/1000ml at 0.32ml/s with 12.2psi vacuum @16:30:18.51 Cartridge 6 sampling 120/1000ml at 0.30ml/s with 13.6psi vacuum @16:31:18.51 Cartridge 6 sampling 138/1000ml at 0.32ml/s with 12.8psi vacuum @16:32:18.51 Cartridge 6 sampling 157/1000ml at 0.30ml/s with 14.5psi vacuum @16:33:18.51 Cartridge 6 sampling 174/1000ml at 0.28ml/s with 14.3psi vacuum @16:34:18.51 Cartridge 6 sampling 192/1000ml at 0.31ml/s with 13.2psi vacuum @16:35:18.51 Cartridge 6 sampling 209/1000ml at 0.27ml/s with 13.0psi vacuum @16:36:18.57 Cartridge 6 sampling 226/1000ml at 0.29ml/s with 13.0psi vacuum @16:37:18.51 Cartridge 6 sampling 242/1000ml at 0.27ml/s with 13.7psi vacuum @16:38:18.51 Cartridge 6 sampling 258/1000ml at 0.26ml/s with 13.7psi vacuum @16:39:18.51 Cartridge 6 sampling 274/1000ml at 0.26ml/s with 12.1psi vacuum @16:40:18.51 Cartridge 6 sampling 289/1000ml at 0.25ml/s with 15.3psi vacuum @16:41:18.51 Cartridge 6 sampling 304/1000ml at 0.25ml/s with 14.1psi vacuum @16:42:18.51 Cartridge 6 sampling 319/1000ml at 0.25ml/s with 14.1psi vacuum @16:42:40.18 Flow averaged 0.29ml/s over 18:23 @16:42:40.21 Waiting up to 6 seconds for vacuum to reduce from 14.1 to 10.0psi @16:42:41.21 Relieved excess vacuum in 2.6 seconds @16:42:41.23 SamplePump.seek 1000ml,45:32 @16:42:43.45 Flow estimate reduced to 0.24ml/s @16:42:43.47 Intake.close @16:42:43.50 Exhaust.close @16:42:43.69 Toroid.reconfigure :hold @16:42:43.76 Toroid.seek :bypass @16:42:45.40 Toroid.reconfigure :default @16:42:45.46 Toroid.seek :clear @16:42:47.46 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @16:42:47.48 Pressure.coast @16:42:47.66 Sampled 325.0ml @16:42:49.90 -> Cmd.startProcessing @16:42:51.77 SPR.command \"read_sequences\" @16:42:52.29 SPR.command \"stats_on\" @16:42:52.33 SPR.command \"start 0 1\" @16:42:54.34 Begun log /var/log/esp/spr19jul2816h.spr @16:42:55.14 Handoff.select :waste @16:42:55.32 Archive.seek :empty @16:43:02.49 Syringe tare is 21% of full scale @16:43:13.07 PinPort.seek :cartridge @16:43:17.63 Lysis1.seek 1350ul @16:43:20.11 Lysis2.seek 1700ul @16:43:23.21 Plunge.seek :reagent @16:43:24.05 Evacuating puck with filter bubble point of 30psi @16:43:24.06 PinPort.seek :cartridge @16:43:24.14 Handoff.select :waste @16:43:31.49 Air pumping 1 strokes to reach 4psi @16:43:31.53 Air.reconfigure :gulp @16:43:31.61 Air.seek :tVent @16:43:33.40 0.0psi after gulp @16:43:33.42 Air stroke #1 of 1 @16:43:33.44 Air.reconfigure :pressurize @16:43:33.52 Air.seek :pressurized @16:43:46.36 0.1psi after pressurize @16:43:46.37 Air.reconfigure :compress @16:43:46.44 Air.seek :evac @16:43:49.11 0.2psi after compress @16:44:03.06 0.1psi after compress (stable) @16:44:03.18 at 0.2psi Opened to waste @16:44:03.19 Handoff.select :blocked @16:44:03.37 Air pumping 10 strokes to reach 30psi @16:44:03.38 Air.reconfigure :gulp @16:44:03.46 Air.seek :tVent @16:44:13.36 0.1psi after gulp @16:44:13.37 Air stroke #1 of 10 @16:44:13.38 Air.reconfigure :pressurize @16:44:13.47 Air.seek :pressurized @16:44:26.31 0.1psi after pressurize @16:44:26.32 Air.reconfigure :compress @16:44:26.41 Air.seek :evac @16:44:29.08 0.2psi after compress @16:44:42.99 0.2psi after compress (stable) @16:44:43.01 Air.reconfigure :gulp @16:44:43.09 Air.seek :tVent @16:44:52.98 0.1psi after gulp @16:44:52.99 Air stroke #2 of 10 @16:44:53.01 Air.reconfigure :pressurize @16:44:53.09 Air.seek :pressurized @16:45:05.94 0.1psi after pressurize @16:45:05.95 Air.reconfigure :compress @16:45:06.03 Air.seek :evac @16:45:08.70 0.3psi after compress @16:45:22.57 0.1psi after compress (stable) @16:45:22.59 Air.reconfigure :gulp @16:45:22.66 Air.seek :tVent @16:45:32.56 0.1psi after gulp @16:45:32.58 Air stroke #3 of 10 @16:45:32.60 Air.reconfigure :pressurize @16:45:32.69 Air.seek :pressurized @16:45:45.53 0.1psi after pressurize @16:45:45.54 Air.reconfigure :compress @16:45:45.62 Air.seek :evac @16:45:48.28 0.2psi after compress @16:46:02.17 0.1psi after compress (stable) @16:46:02.19 Air.reconfigure :gulp @16:46:02.27 Air.seek :tVent @16:46:12.16 0.1psi after gulp @16:46:12.17 Air stroke #4 of 10 @16:46:12.19 Air.reconfigure :pressurize @16:46:12.27 Air.seek :pressurized @16:46:25.11 0.1psi after pressurize @16:46:25.12 Air.reconfigure :compress @16:46:25.21 Air.seek :evac @16:46:27.88 0.2psi after compress @16:46:41.83 0.1psi after compress (stable) @16:46:41.85 Air.reconfigure :gulp @16:46:41.93 Air.seek :tVent @16:46:51.82 0.2psi after gulp @16:46:51.83 Air stroke #5 of 10 @16:46:51.84 Air.reconfigure :pressurize @16:46:51.93 Air.seek :pressurized @16:47:04.77 0.1psi after pressurize @16:47:04.78 Air.reconfigure :compress @16:47:04.86 Air.seek :evac @16:47:07.52 0.2psi after compress @16:47:21.43 0.1psi after compress (stable) @16:47:21.45 Air.reconfigure :gulp @16:47:21.54 Air.seek :tVent @16:47:31.43 0.1psi after gulp @16:47:31.44 Air stroke #6 of 10 @16:47:31.46 Air.reconfigure :pressurize @16:47:31.54 Air.seek :pressurized @16:47:44.38 0.1psi after pressurize @16:47:44.39 Air.reconfigure :compress @16:47:44.48 Air.seek :evac @16:47:47.14 0.2psi after compress @16:48:01.03 0.1psi after compress (stable) @16:48:01.05 Air.reconfigure :gulp @16:48:01.14 Air.seek :tVent @16:48:11.04 0.1psi after gulp @16:48:11.05 Air stroke #7 of 10 @16:48:11.07 Air.reconfigure :pressurize @16:48:11.15 Air.seek :pressurized @16:48:24.00 0.1psi after pressurize @16:48:24.01 Air.reconfigure :compress @16:48:24.09 Air.seek :evac @16:48:26.79 0.2psi after compress @16:48:40.70 0.1psi after compress (stable) @16:48:40.72 Air.reconfigure :gulp @16:48:40.80 Air.seek :tVent @16:48:50.72 0.1psi after gulp @16:48:50.73 Air stroke #8 of 10 @16:48:50.75 Air.reconfigure :pressurize @16:48:50.83 Air.seek :pressurized @16:49:03.67 0.1psi after pressurize @16:49:03.68 Air.reconfigure :compress @16:49:03.76 Air.seek :evac @16:49:06.45 0.3psi after compress @16:49:20.45 0.1psi after compress (stable) @16:49:20.47 Air.reconfigure :gulp @16:49:20.56 Air.seek :tVent @16:49:30.46 0.1psi after gulp @16:49:30.47 Air stroke #9 of 10 @16:49:30.49 Air.reconfigure :pressurize @16:49:30.57 Air.seek :pressurized @16:49:43.42 0.1psi after pressurize @16:49:43.43 Air.reconfigure :compress @16:49:43.52 Air.seek :evac @16:49:46.19 0.2psi after compress @16:50:00.09 0.1psi after compress (stable) @16:50:00.11 Air.reconfigure :gulp @16:50:00.18 Air.seek :tVent @16:50:10.08 0.1psi after gulp @16:50:10.09 Air stroke #10 of 10 @16:50:10.11 Air.reconfigure :pressurize @16:50:10.18 Air.seek :pressurized @16:50:23.05 0.2psi after pressurize @16:50:23.06 Air.reconfigure :compress @16:50:23.15 Air.seek :evac @16:50:25.85 0.3psi after compress @16:50:39.75 0.1psi after compress (stable) @16:50:39.83 Handoff.select :blocked @16:50:39.84 Syringe::Underpressure in PROCESSING -- Reached only 0.1 of 30.0psi after pumping 15575ul @16:50:39.86 Skipping SPR because lysate volume unknown @16:50:39.88 SPR.command \"start 2 14\" @16:50:40.49 Handoff.select :waste @16:50:40.94 Ended log /var/log/esp/spr19jul2816h.spr @16:50:40.95 SPR.command \"stats_off\" @16:50:43.81 -> Cmd.stop @16:50:43.90 Handoff.select :close @16:50:44.02 Gate.power :heat,:OFF @16:50:44.04 Gate.power :cartridge,:OFF @16:50:44.12 Carousel.seek 6 @16:50:44.29 Plunge.seek :locked @16:50:45.13 PinPort.seek :clear @16:50:49.74 Archive.seek :clear @16:50:49.87 Air.reconfigure :default @16:50:50.00 Lysis1.seek :clear @16:50:50.10 Air.seek :clear @16:50:50.18 Lysis2.seek :clear @16:51:07.50 Archive.coast @16:51:07.55 Air.coast @16:51:07.60 Lysis1.coast @16:51:07.65 Lysis2.coast @16:51:07.70 Plunge.coast @16:51:07.75 SamplePump.coast @16:51:07.80 Toroid.coast @16:51:07.85 Carousel.coast @16:51:07.90 PinPort.coast @16:51:08.08 Gate.power :motor,:OFF @16:51:08.09 Gate.power :core,:OFF @16:51:08.12 Gate.power :analytic1,:OFF @16:51:08.14 Gate.power :analytic2,:OFF @16:51:08.16 Gate.power :analytic3,:OFF @16:51:08.18 Gate.power :main,:OFF @16:51:08.20 Safely stopped and ready to power off ##-- file closed 2019-07-29T00:32:28.183Z ##-- file opened 2019-07-29T00:32:28.185Z @17:32:28.06 -> showStatus @17:32:30.15 -> Cmd.loadCartridge -9 @17:32:30.28 Gate.power :main,:ON,limit to 5000mA @17:32:30.29 Gate.power :analytic1,:ON,limit to 5000mA @17:32:30.32 Gate.power :analytic2,:ON,limit to 5000mA @17:32:30.34 Gate.power :analytic3,:ON,limit to 5000mA @17:32:30.47 Powering core bus @17:32:32.76 SPR Module 1, FW Rev. B, FPGA Rev. C @17:32:32.88 Archive.configure :default @17:32:33.00 Air.configure :default @17:32:33.13 Lysis1.configure :default @17:32:33.23 Lysis2.configure :default @17:32:33.35 Plunge.configure :default @17:32:33.47 SamplePump.configure :default @17:32:33.58 ExtraPump.configure :default @17:32:33.70 Toroid.configure :default @17:32:33.82 Carousel.configure :default @17:32:33.94 Pressure.configure :default @17:32:34.03 PinPort.configure :default @17:32:34.09 All elf controllers running firmware version 4.58 @17:32:34.12 Gate.power :motor,:ON,limit to 5000mA @17:32:34.27 Archive.reconfigure :default @17:32:34.56 Archive.home.jog 0 @17:32:34.69 Toroid.reconfigure :default @17:32:34.78 Plunge.reconfigure :default @17:32:34.82 Lysis2.reconfigure :default @17:32:34.85 Lysis1.reconfigure :default @17:32:34.91 Air.reconfigure :default @17:32:34.98 PinPort.reconfigure :default @17:32:35.18 Air.home.jog 0 @17:32:35.23 Plunge.home.jog 0 @17:32:35.29 Lysis1.home.jog 0 @17:32:35.35 Toroid.home.jog 0 @17:32:35.40 PinPort.home.jog 0 @17:32:35.46 Lysis2.home.jog 0 @17:32:35.74 Archive.reconfigure :homing @17:32:35.98 Toroid.reconfigure :homing @17:32:36.00 Plunge.reconfigure :homing @17:32:36.03 Air.reconfigure :homing @17:32:36.08 PinPort.reconfigure :homing @17:32:36.11 Lysis2.reconfigure :homing @17:32:36.15 Lysis1.reconfigure :homing @17:32:36.35 Lysis2.jog 162 @17:32:36.41 PinPort.jog -106 @17:32:36.46 Plunge.jog 1561 @17:32:36.52 Air.jog 151 @17:32:36.58 Archive.jog 1409 @17:32:36.64 Toroid.jog 629 @17:32:36.69 Lysis1.jog 1407 @17:32:36.77 PinPort.setRawPosition! 0 @17:32:36.82 PinPort.seek :home @17:32:36.91 PinPort.reconfigure :default @17:32:36.94 Lysis2.setRawPosition! 50 @17:32:37.01 Lysis2.seek :home @17:32:37.12 Air.setRawPosition! 50 @17:32:37.16 Air.seek :home @17:32:37.32 Lysis2.reconfigure :default @17:32:37.44 Air.reconfigure :default @17:32:37.68 Plunge.setRawPosition! 50 @17:32:37.73 Plunge.seek :home @17:32:37.82 Archive.setRawPosition! 50 @17:32:37.87 Archive.seek :home @17:32:37.95 Lysis1.setRawPosition! 50 @17:32:38.01 Lysis1.seek :home @17:32:38.09 Plunge.reconfigure :default @17:32:38.17 Archive.reconfigure :default @17:32:38.29 Lysis1.reconfigure :default @17:32:38.77 Toroid.setRawPosition! 0 @17:32:38.81 Toroid.seek :home @17:32:38.90 Toroid.reconfigure :default @17:32:39.02 Toroid.seek :clear @17:32:40.64 Carousel.reconfigure :default @17:32:40.71 Carousel.home.jog 0 @17:32:41.20 Carousel.seek 6 @17:32:41.35 Selecting Cartridge 5 @17:32:41.60 Carousel.seek 5 @17:32:42.08 Lysis1.seek :locked @17:32:46.21 Plunge.seek :locked @17:32:46.33 Air.seek :locked @17:32:50.46 Powering cartridge bus @17:32:52.59 Gate.setIR 1750,5000,0,6 @17:32:52.72 Gate.setIR 0,5000,0,6 @17:32:52.77 Loaded Lyse-n-Go Cartridge @17:32:52.79 PuckHeater.configure HeatConfig @17:32:52.84 Gate.power :heat,:ON,limit to 5000mA @17:33:09.01 -> Cmd.startFiltering @17:33:09.24 Duration of filtering limited to 2:05:00 @17:33:09.49 Priming sample loop w/100ml, bypass w/10ml @17:33:09.50 SamplePump.setPosition! 0ml @17:33:09.54 Bypass.open @17:33:11.03 Intake.open @17:33:11.83 Sea 2.6m deep (18.5psia) @17:33:11.84 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:33:15.06 Vacuum tare is 52% of full scale @17:33:15.19 Pressure.reconfigure :default @17:33:15.25 Pressure.hold @17:33:15.41 SamplePump.reconfigure :prime @17:33:15.49 SamplePump.seek 10ml,1:10 @17:33:24.44 Pressure.coast @17:33:24.49 SamplePump.setPosition! 0ml @17:33:24.54 Exhaust.open @17:33:25.94 Bypass.close @17:33:27.48 Pressure.reconfigure :default @17:33:27.54 Pressure.hold @17:33:27.66 Sea 2.2m deep (18.0psia) @17:33:27.79 SamplePump.reconfigure :prime @17:33:27.86 SamplePump.seek 100ml,7:10 @17:35:23.69 Pressure.coast @17:35:23.75 Bypass.open @17:35:24.48 Intake.close @17:35:25.31 Toroid.reconfigure :hold @17:35:25.37 Toroid.seek :crack @17:35:26.17 Toroid.reconfigure :default @17:35:26.23 Toroid.seek :clear @17:35:27.79 Toroid.reconfigure :hold @17:35:27.86 Toroid.seek :cartridge @17:35:29.68 Toroid.reconfigure :default @17:35:29.74 Toroid.seek :clear @17:35:31.70 Pressurizing cartridge to 14psi above 18.1psia @17:35:31.71 SamplePump.setPosition! 0ml @17:35:31.76 Intake.open @17:35:31.95 Bypass.close @17:35:32.66 Exhaust.close @17:35:33.45 Pressure.reconfigure :default @17:35:33.51 Pressure.hold @17:35:33.68 SamplePump.reconfigure :charge @17:35:33.75 SamplePump.seek 2.3ml,41 seconds @17:35:43.38 Pressure.coast @17:35:43.44 Intake.close @17:35:44.28 Pressurized cartridge with 0.434ml to 14.0psi @17:35:44.29 Isolated @29.8psia (11.7psi) @17:35:44.30 Checking for cartridge leaks from 29.8psia @17:35:44.33 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @17:35:49.37 Cartridge pressure fell 4% to 28.6psia @17:35:49.45 Sampling 1000.0ml @17:35:49.46 SamplePump.setPosition! 0ml @17:35:49.51 Exhaust.open @17:35:50.20 Intake.open @17:35:51.07 Pressure.reconfigure :default @17:35:51.12 Pressure.hold @17:35:51.23 Sea 2.7m deep (18.7psia) @17:35:51.36 SamplePump.reconfigure :sample @17:35:51.44 SamplePump.seek 1000ml,1:07:09 @17:35:54.11 Cartridge 5 sampling 1/1000ml at 0.38ml/s with 12.6psi vacuum @17:36:54.11 Cartridge 5 sampling 22/1000ml at 0.33ml/s with 14.7psi vacuum @17:37:54.11 Cartridge 5 sampling 43/1000ml at 0.34ml/s with 13.6psi vacuum @17:38:54.11 Cartridge 5 sampling 63/1000ml at 0.35ml/s with 13.0psi vacuum @17:39:54.11 Cartridge 5 sampling 83/1000ml at 0.34ml/s with 12.9psi vacuum @17:40:54.11 Cartridge 5 sampling 103/1000ml at 0.33ml/s with 12.9psi vacuum @17:41:54.11 Cartridge 5 sampling 123/1000ml at 0.34ml/s with 12.2psi vacuum @17:42:54.11 Cartridge 5 sampling 143/1000ml at 0.33ml/s with 13.5psi vacuum @17:43:54.11 Cartridge 5 sampling 162/1000ml at 0.30ml/s with 12.8psi vacuum @17:44:54.11 Cartridge 5 sampling 181/1000ml at 0.31ml/s with 15.2psi vacuum @17:45:54.11 Cartridge 5 sampling 200/1000ml at 0.32ml/s with 12.1psi vacuum @17:46:54.11 Cartridge 5 sampling 218/1000ml at 0.29ml/s with 13.1psi vacuum @17:47:54.11 Cartridge 5 sampling 236/1000ml at 0.32ml/s with 13.2psi vacuum @17:48:54.11 Cartridge 5 sampling 254/1000ml at 0.30ml/s with 12.4psi vacuum @17:49:54.11 Cartridge 5 sampling 272/1000ml at 0.28ml/s with 14.7psi vacuum @17:50:54.11 Cartridge 5 sampling 289/1000ml at 0.29ml/s with 13.3psi vacuum @17:51:54.11 Cartridge 5 sampling 306/1000ml at 0.27ml/s with 10.8psi vacuum @17:52:54.11 Cartridge 5 sampling 322/1000ml at 0.26ml/s with 14.1psi vacuum @17:53:54.11 Cartridge 5 sampling 338/1000ml at 0.25ml/s with 15.3psi vacuum @17:54:54.11 Cartridge 5 sampling 354/1000ml at 0.28ml/s with 13.5psi vacuum @17:55:54.11 Cartridge 5 sampling 370/1000ml at 0.25ml/s with 12.3psi vacuum @17:56:54.11 Cartridge 5 sampling 386/1000ml at 0.25ml/s with 12.1psi vacuum @17:57:54.10 Cartridge 5 sampling 401/1000ml at 0.26ml/s with 12.9psi vacuum @17:58:54.11 Cartridge 5 sampling 417/1000ml at 0.25ml/s with 13.4psi vacuum @17:59:54.11 Cartridge 5 sampling 432/1000ml at 0.25ml/s with 15.3psi vacuum @18:00:54.11 Cartridge 5 sampling 447/1000ml at 0.25ml/s with 15.5psi vacuum @18:01:31.52 Flow averaged 0.30ml/s over 25:33 @18:01:31.55 Waiting up to 6 seconds for vacuum to reduce from 14.2 to 10.0psi @18:01:32.24 Relieved excess vacuum in 8 seconds @18:01:32.27 SamplePump.seek 1000ml,36:45 @18:01:54.11 Cartridge 5 sampling 461/1000ml at 0.25ml/s with 15.1psi vacuum @18:02:00.90 Flow averaged 0.32ml/s over 22 seconds @18:02:00.93 Waiting up to 6 seconds for vacuum to reduce from 14.0 to 10.0psi @18:02:01.63 Relieved excess vacuum in 7.4 seconds @18:02:01.65 SamplePump.seek 1000ml,36:17 @18:02:39.90 Flow averaged 0.30ml/s over 32 seconds @18:02:39.93 Waiting up to 8 seconds for vacuum to reduce from 15.0 to 10.0psi @18:02:40.63 Relieved excess vacuum in 7.3 seconds @18:02:40.65 SamplePump.seek 1000ml,35:39 @18:02:46.09 Flow estimate reduced to 0.24ml/s @18:02:46.10 Intake.close @18:02:46.15 Exhaust.close @18:02:46.33 Toroid.reconfigure :hold @18:02:46.39 Toroid.seek :bypass @18:02:48.01 Toroid.reconfigure :default @18:02:48.08 Toroid.seek :clear @18:02:49.70 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @18:02:49.72 Pressure.coast @18:02:49.90 Sampled 474.0ml @18:02:52.11 -> Cmd.startProcessing @18:02:53.95 SPR.command \"read_sequences\" @18:02:54.32 SPR.command \"stats_on\" @18:02:54.36 SPR.command \"start 0 1\" @18:02:56.37 Begun log /var/log/esp/spr19jul2818h.spr @18:02:57.18 Handoff.select :waste @18:02:57.36 Archive.seek :empty @18:03:04.55 Syringe tare is 21% of full scale @18:03:15.09 PinPort.seek :cartridge @18:03:19.65 Lysis1.seek 1350ul @18:03:22.12 Lysis2.seek 1700ul @18:03:25.22 Plunge.seek :reagent @18:03:26.06 Evacuating puck with filter bubble point of 30psi @18:03:26.07 PinPort.seek :cartridge @18:03:26.15 Handoff.select :waste @18:03:33.47 Air pumping 1 strokes to reach 4psi @18:03:33.48 Air.reconfigure :gulp @18:03:33.57 Air.seek :tVent @18:03:35.37 0.1psi after gulp @18:03:35.38 Air stroke #1 of 1 @18:03:35.40 Air.reconfigure :pressurize @18:03:35.49 Air.seek :pressurized @18:03:48.32 0.1psi after pressurize @18:03:48.33 Air.reconfigure :compress @18:03:48.40 Air.seek :evac @18:03:51.07 0.2psi after compress @18:04:04.96 0.2psi after compress (stable) @18:04:05.08 at 0.2psi Opened to waste @18:04:05.09 Handoff.select :blocked @18:04:05.26 Air pumping 10 strokes to reach 30psi @18:04:05.28 Air.reconfigure :gulp @18:04:05.36 Air.seek :tVent @18:04:15.26 4.8psi after gulp @18:04:15.27 Air stroke #1 of 10 @18:04:15.30 Air.reconfigure :pressurize @18:04:15.38 Air.seek :pressurized @18:04:28.22 6.8psi after pressurize @18:04:28.23 Air.reconfigure :compress @18:04:28.31 Air.seek :evac @18:04:30.97 8.0psi after compress @18:04:53.80 Reached 30.0 of 30.0psi after pumping 3115ul (2.00 strokes) @18:04:53.81 Handoff.select :waste @18:05:02.30 delay 1:00 @18:06:02.32 Handoff.select :blocked @18:06:27.33 Handoff.select :waste @18:06:27.44 PSI at @0.98psi at evacEnd @18:06:27.45 Handoff.select :blocked @18:06:27.58 Air.reconfigure :gulp @18:06:27.66 Air.seek :tVent @18:06:44.76 Stable @1.5psi after Handoff blocked @18:06:44.84 Air pumping 10 strokes to reach 27psi @18:06:44.85 Air stroke #1 of 10 @18:06:44.88 Air.reconfigure :pressurize @18:06:44.95 Air.seek :pressurized @18:06:57.79 1.9psi after pressurize @18:06:57.80 Air.reconfigure :compress @18:06:57.90 Air.seek :evac @18:07:00.56 2.0psi after compress @18:08:15.08 15.8psi after compress (stable) @18:08:15.10 Air.reconfigure :gulp @18:08:15.17 Air.seek :tVent @18:08:25.07 17.7psi after gulp @18:08:25.08 Air stroke #2 of 10 @18:08:25.10 Air.reconfigure :pressurize @18:08:25.18 Air.seek :pressurized @18:08:38.01 16.7psi after pressurize @18:08:38.02 Air.reconfigure :compress @18:08:38.11 Air.seek :evac @18:08:40.79 16.4psi after compress @18:09:19.00 Reached 26.6 of 26.5psi after pumping 4672ul (3.00 strokes) @18:09:19.02 Handoff.select :waste @18:09:36.90 delay 1:00 @18:10:36.93 Handoff.select :blocked @18:11:01.93 Handoff.select :waste @18:11:02.05 PSI at @0.06psi at evacEnd @18:11:02.06 Air.reconfigure :default @18:11:02.15 Air.seek :fVent @18:11:03.60 delay 5 seconds @18:11:08.62 Seawater evacuated, Presure above/below filter released. Lysis begins @18:11:08.69 Lysis1.reconfigure :slow @18:11:08.75 Lysis1.seek 1050ul @18:11:38.79 delay 5 seconds @18:11:43.82 Handoff.select :blocked @18:11:43.98 Lysis1.seek 450ul @18:12:43.96 delay 3 seconds @18:12:47.04 Air.reconfigure to mix into 1.37806 parts Lysis1 @18:12:47.51 Air.seek :evac @18:12:47.55 Lysis1.seek 300ul @18:13:02.68 Air.reconfigure :default @18:13:02.74 Air.seek :full @18:13:16.96 Lysis1.seek :empty @18:13:47.08 Lysis Delivered. Syringe pressure @38.7psi @18:13:47.13 PuckHeater.seek 85.6C @18:14:51.35 PuckHeater.hold 15:00,85.6C @18:29:52.44 Lysis complete. Syringe pressure @46.0psi @18:29:52.45 Handoff.select :blocked @18:29:52.55 Lysis1.reconfigure :halfSpeed @18:29:52.61 Lysis1.seek 200ul @18:29:56.38 delay 1 second @18:29:57.40 Lysis1.seek :empty @18:30:01.22 Initial AirS pressure @48.4psi @18:30:01.28 Lysis1.reconfigure :extract @18:30:01.37 Lysis1.seek 875ul,10:00 @18:30:02.53 Air pumping 10 strokes to reach 21psi @18:30:02.55 Air stroke #1 of 10 @18:30:02.58 Air.reconfigure :pressurize @18:30:02.65 Air.seek :pressurized @18:30:15.49 15.1psi after pressurize @18:30:15.50 Air.reconfigure :compress @18:30:15.94 Air.seek :evac @18:30:18.60 20.3psi after compress @18:30:32.50 15.8psi after compress (stable) @18:30:32.52 Air.reconfigure :gulp @18:30:32.59 Air.seek :tVent @18:30:42.49 16.1psi after gulp @18:30:42.50 Air stroke #2 of 10 @18:30:42.51 Air.reconfigure :pressurize @18:30:42.61 Air.seek :pressurized @18:30:55.44 15.8psi after pressurize @18:30:55.45 Air.reconfigure :compress @18:30:55.54 Air.seek :evac @18:30:58.21 17.1psi after compress @18:31:12.15 15.8psi after compress (stable) @18:31:12.17 Air.reconfigure :gulp @18:31:12.24 Air.seek :tVent @18:31:22.13 15.9psi after gulp @18:31:22.14 Air stroke #3 of 10 @18:31:22.15 Air.reconfigure :pressurize @18:31:22.23 Air.seek :pressurized @18:31:35.07 15.8psi after pressurize @18:31:35.08 Air.reconfigure :compress @18:31:35.16 Air.seek :evac @18:31:37.82 16.2psi after compress @18:31:40.03 Air.reconfigure :default @18:31:40.09 Air.hold @18:31:40.26 Lysis1 at 875ul pressure @15.5psi @18:31:40.27 Lysis1.reconfigure :default @18:31:40.34 Lysis1.seek 900ul @18:31:41.11 Lysis1 at 900ul pressure(finPSI) @11.7psi @18:31:41.12 delay 1 second @18:31:42.25 Lysis1 at 900ul pressure @14.5psi @18:31:42.27 Air.reconfigure :halfSpeed @18:31:42.34 Air.seek :fVent @18:31:44.59 Puck Top Vented. Syringe pressure @4.2psi @18:31:44.60 Handoff.select :waste @18:31:44.73 delay 5 seconds @18:31:49.75 Lysate Recovered in LyS1 @18:31:49.76 Lysis1.reconfigure :DAmix @18:31:49.87 Lysis2.reconfigure :DAmix @18:31:50.32 Lysis1.seek 650ul @18:31:50.41 Lysis2.seek 1450ul @18:32:25.47 delay 5 seconds @18:32:30.49 Handoff.select SPR @18:32:30.60 SPR.command \"start 2 14\" @18:32:32.12 delay 10 seconds @18:32:42.14 SPR.command \"start 1 0\" @18:32:44.90 Waiting up to 4:00 for SPR to enter state 1 @18:35:17.53 SPR.command \"hold_on\" @18:35:18.96 SPR Dilution 1 of 1 -- Running Experiment 1 @18:35:19.37 Lysis1.seek 350ul @18:35:19.42 Lysis2.seek 1150ul @18:36:01.47 volL1 = 300.0 and volL2 = 300.0 @18:36:01.48 delay 15 seconds @18:36:16.51 SPR.command \"hold_off\" @18:36:19.62 Waiting up to 25:00 for SPR to enter state 7 @18:56:33.45 SPR Analysis has Completed @18:56:33.47 Handoff.select :waste @18:56:34.39 Ended log /var/log/esp/spr19jul2818h.spr @18:56:34.40 SPR.command \"stats_off\" @18:56:36.03 -> Cmd.stop @18:56:36.12 Handoff.select :close @18:56:36.24 Gate.power :heat,:OFF @18:56:36.26 Gate.power :cartridge,:OFF @18:56:36.34 Carousel.seek 5 @18:56:36.51 Plunge.seek :locked @18:56:37.35 PinPort.seek :clear @18:56:41.97 Archive.seek :clear @18:56:42.10 Air.reconfigure :default @18:56:42.23 Lysis1.reconfigure :default @18:56:42.33 Air.seek :clear @18:56:42.39 Lysis1.seek :clear @18:56:42.48 Lysis2.reconfigure :default @18:56:42.55 Lysis2.seek :clear @18:56:59.72 Archive.coast @18:56:59.77 Air.coast @18:56:59.82 Lysis1.coast @18:56:59.87 Lysis2.coast @18:56:59.93 Plunge.coast @18:57:00.30 SamplePump.coast @18:57:00.36 Toroid.coast @18:57:00.42 Carousel.coast @18:57:00.47 PinPort.coast @18:57:00.65 Gate.power :motor,:OFF @18:57:00.67 Gate.power :core,:OFF @18:57:00.69 Gate.power :analytic1,:OFF @18:57:00.71 Gate.power :analytic2,:OFF @18:57:00.73 Gate.power :analytic3,:OFF @18:57:00.75 Gate.power :main,:OFF @18:57:00.77 Safely stopped and ready to power off ##-- file closed 2019-07-29T02:55:23.133Z ##-- file opened 2019-07-29T02:55:23.135Z @19:55:23.10 -> showStatus @19:55:25.18 -> Cmd.loadCartridge -9 @19:55:25.31 Gate.power :main,:ON,limit to 5000mA @19:55:25.32 Gate.power :analytic1,:ON,limit to 5000mA @19:55:25.34 Gate.power :analytic2,:ON,limit to 5000mA @19:55:25.37 Gate.power :analytic3,:ON,limit to 5000mA @19:55:25.50 Powering core bus @19:55:27.78 SPR Module 1, FW Rev. B, FPGA Rev. C @19:55:27.93 Archive.configure :default @19:55:28.07 Air.configure :default @19:55:28.17 Lysis1.configure :default @19:55:28.27 Lysis2.configure :default @19:55:28.39 Plunge.configure :default @19:55:28.51 SamplePump.configure :default @19:55:28.63 ExtraPump.configure :default @19:55:28.74 Toroid.configure :default @19:55:28.86 Carousel.configure :default @19:55:28.98 Pressure.configure :default @19:55:29.08 PinPort.configure :default @19:55:29.14 All elf controllers running firmware version 4.58 @19:55:29.16 Gate.power :motor,:ON,limit to 5000mA @19:55:29.29 Archive.reconfigure :default @19:55:29.62 Archive.home.jog 0 @19:55:29.76 Toroid.reconfigure :default @19:55:29.84 Plunge.reconfigure :default @19:55:29.87 Lysis2.reconfigure :default @19:55:29.90 Air.reconfigure :default @19:55:29.95 PinPort.reconfigure :default @19:55:30.03 Lysis1.reconfigure :default @19:55:30.23 Lysis1.home.jog 0 @19:55:30.28 Plunge.home.jog 0 @19:55:30.34 Toroid.home.jog 0 @19:55:30.40 PinPort.home.jog 0 @19:55:30.46 Air.home.jog 0 @19:55:30.52 Lysis2.home.jog 0 @19:55:30.85 Archive.reconfigure :homing @19:55:31.05 Toroid.reconfigure :homing @19:55:31.07 Plunge.reconfigure :homing @19:55:31.10 Air.reconfigure :homing @19:55:31.14 PinPort.reconfigure :homing @19:55:31.17 Lysis1.reconfigure :homing @19:55:31.21 Lysis2.reconfigure :homing @19:55:31.42 PinPort.jog -106 @19:55:31.48 Lysis2.jog 162 @19:55:31.53 Plunge.jog 1544 @19:55:31.59 Air.jog 1416 @19:55:31.65 Toroid.jog 631 @19:55:31.71 Archive.jog 1406 @19:55:31.77 Lysis1.jog 1407 @19:55:31.85 PinPort.setRawPosition! 0 @19:55:31.90 PinPort.seek :home @19:55:31.99 PinPort.reconfigure :default @19:55:32.02 Lysis2.setRawPosition! 50 @19:55:32.10 Lysis2.seek :home @19:55:32.39 Lysis2.reconfigure :default @19:55:32.77 Plunge.setRawPosition! 50 @19:55:32.79 Air.setRawPosition! 50 @19:55:32.87 Plunge.seek :home @19:55:32.95 Air.seek :home @19:55:33.07 Lysis1.setRawPosition! 50 @19:55:33.09 Archive.setRawPosition! 50 @19:55:33.15 Lysis1.seek :home @19:55:33.19 Archive.seek :home @19:55:33.33 Air.reconfigure :default @19:55:33.35 Plunge.reconfigure :default @19:55:33.51 Lysis1.reconfigure :default @19:55:33.58 Archive.reconfigure :default @19:55:33.80 Toroid.setRawPosition! 0 @19:55:33.84 Toroid.seek :home @19:55:33.94 Toroid.reconfigure :default @19:55:34.08 Toroid.seek :clear @19:55:35.70 Carousel.reconfigure :default @19:55:35.77 Carousel.home.jog 0 @19:55:36.26 Carousel.seek 5 @19:55:36.42 Selecting Cartridge 4 @19:55:36.67 Carousel.seek 4 @19:55:37.15 Lysis1.seek :locked @19:55:41.28 Plunge.seek :locked @19:55:41.40 Air.seek :locked @19:55:45.53 Powering cartridge bus @19:55:47.65 Gate.setIR 1750,5000,0,6 @19:55:47.78 Gate.setIR 0,5000,0,6 @19:55:47.83 Loaded Lyse-n-Go Cartridge @19:55:47.85 PuckHeater.configure HeatConfig @19:55:47.91 Gate.power :heat,:ON,limit to 5000mA @19:56:03.96 -> Cmd.startFiltering @19:56:04.18 Duration of filtering limited to 2:05:00 @19:56:04.44 Priming sample loop w/100ml, bypass w/10ml @19:56:04.45 SamplePump.setPosition! 0ml @19:56:04.49 Bypass.open @19:56:05.94 Intake.open @19:56:06.78 Sea 3.2m deep (19.4psia) @19:56:06.80 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @19:56:10.00 Vacuum tare is 52% of full scale @19:56:10.13 Pressure.reconfigure :default @19:56:10.19 Pressure.hold @19:56:10.36 SamplePump.reconfigure :prime @19:56:10.43 SamplePump.seek 10ml,1:10 @19:56:19.38 Pressure.coast @19:56:19.43 SamplePump.setPosition! 0ml @19:56:19.48 Exhaust.open @19:56:20.88 Bypass.close @19:56:22.40 Pressure.reconfigure :default @19:56:22.46 Pressure.hold @19:56:22.58 Sea 3.8m deep (20.3psia) @19:56:22.71 SamplePump.reconfigure :prime @19:56:22.78 SamplePump.seek 100ml,7:10 @19:58:18.15 Pressure.coast @19:58:18.20 Bypass.open @19:58:18.92 Intake.close @19:58:19.77 Toroid.reconfigure :hold @19:58:19.83 Toroid.seek :crack @19:58:20.63 Toroid.reconfigure :default @19:58:20.69 Toroid.seek :clear @19:58:22.26 Toroid.reconfigure :hold @19:58:22.32 Toroid.seek :cartridge @19:58:24.14 Toroid.reconfigure :default @19:58:24.20 Toroid.seek :clear @19:58:26.17 Pressurizing cartridge to 14psi above 18.8psia @19:58:26.18 SamplePump.setPosition! 0ml @19:58:26.22 Intake.open @19:58:26.96 Bypass.close @19:58:27.65 Exhaust.close @19:58:28.42 Pressure.reconfigure :default @19:58:28.47 Pressure.hold @19:58:28.64 SamplePump.reconfigure :charge @19:58:28.71 SamplePump.seek 2.3ml,41 seconds @19:58:37.82 Pressure.coast @19:58:37.87 Intake.close @19:58:38.70 Pressurized cartridge with 0.388ml to 14.5psi @19:58:38.71 Isolated @31.2psia (12.4psi) @19:58:38.72 Checking for cartridge leaks from 31.2psia @19:58:38.75 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @19:58:43.85 Sampling 1000.0ml @19:58:43.87 SamplePump.setPosition! 0ml @19:58:43.92 Exhaust.open @19:58:44.62 Intake.open @19:58:44.96 Pressure.reconfigure :default @19:58:45.02 Pressure.hold @19:58:45.13 Sea 4.4m deep (21.1psia) @19:58:45.27 SamplePump.reconfigure :sample @19:58:45.34 SamplePump.seek 1000ml,1:07:09 @19:58:48.00 Cartridge 4 sampling 1/1000ml at 0.37ml/s with 12.3psi vacuum @19:59:48.00 Cartridge 4 sampling 22/1000ml at 0.35ml/s with 13.3psi vacuum @20:00:48.00 Cartridge 4 sampling 44/1000ml at 0.36ml/s with 12.2psi vacuum 1.8m deep (17.3psia) @20:01:48.00 Cartridge 4 sampling 65/1000ml at 0.36ml/s with 12.7psi vacuum @20:02:48.00 Cartridge 4 sampling 86/1000ml at 0.35ml/s with 13.3psi vacuum 1.6m deep (17.1psia) @20:03:48.00 Cartridge 4 sampling 108/1000ml at 0.36ml/s with 12.8psi vacuum @20:04:48.00 Cartridge 4 sampling 129/1000ml at 0.37ml/s with 14.6psi vacuum @20:05:48.00 Cartridge 4 sampling 150/1000ml at 0.35ml/s with 13.0psi vacuum @20:06:48.00 Cartridge 4 sampling 170/1000ml at 0.34ml/s with 13.3psi vacuum @20:07:48.00 Cartridge 4 sampling 191/1000ml at 0.35ml/s with 13.1psi vacuum @20:08:48.00 Cartridge 4 sampling 212/1000ml at 0.35ml/s with 12.9psi vacuum @20:09:48.00 Cartridge 4 sampling 232/1000ml at 0.34ml/s with 12.6psi vacuum @20:10:48.00 Cartridge 4 sampling 252/1000ml at 0.33ml/s with 12.3psi vacuum @20:11:48.00 Cartridge 4 sampling 272/1000ml at 0.34ml/s with 13.0psi vacuum @20:12:48.00 Cartridge 4 sampling 292/1000ml at 0.33ml/s with 12.2psi vacuum @20:13:48.00 Cartridge 4 sampling 312/1000ml at 0.33ml/s with 12.1psi vacuum @20:14:48.00 Cartridge 4 sampling 331/1000ml at 0.33ml/s with 13.2psi vacuum @20:15:48.00 Cartridge 4 sampling 351/1000ml at 0.31ml/s with 13.2psi vacuum @20:16:48.00 Cartridge 4 sampling 370/1000ml at 0.31ml/s with 13.0psi vacuum @20:17:48.00 Cartridge 4 sampling 389/1000ml at 0.31ml/s with 15.0psi vacuum @20:18:48.00 Cartridge 4 sampling 408/1000ml at 0.30ml/s with 14.1psi vacuum @20:19:48.00 Cartridge 4 sampling 426/1000ml at 0.31ml/s with 11.7psi vacuum 1.7m deep (17.2psia) @20:20:48.00 Cartridge 4 sampling 445/1000ml at 0.29ml/s with 12.7psi vacuum @20:21:48.00 Cartridge 4 sampling 463/1000ml at 0.32ml/s with 15.3psi vacuum @20:22:48.00 Cartridge 4 sampling 481/1000ml at 0.30ml/s with 12.4psi vacuum @20:23:48.00 Cartridge 4 sampling 498/1000ml at 0.28ml/s with 10.2psi vacuum @20:24:48.00 Cartridge 4 sampling 516/1000ml at 0.30ml/s with 11.7psi vacuum @20:25:48.00 Cartridge 4 sampling 533/1000ml at 0.27ml/s with 12.3psi vacuum @20:26:48.38 Cartridge 4 sampling 550/1000ml at 0.27ml/s with 13.2psi vacuum @20:27:48.00 Cartridge 4 sampling 566/1000ml at 0.28ml/s with 11.3psi vacuum @20:28:48.00 Cartridge 4 sampling 583/1000ml at 0.27ml/s with 12.1psi vacuum @20:29:48.00 Cartridge 4 sampling 599/1000ml at 0.28ml/s with 10.9psi vacuum @20:30:48.00 Cartridge 4 sampling 615/1000ml at 0.26ml/s with 12.2psi vacuum @20:31:48.00 Cartridge 4 sampling 631/1000ml at 0.28ml/s with 14.6psi vacuum @20:32:48.00 Cartridge 4 sampling 647/1000ml at 0.25ml/s with 14.2psi vacuum @20:33:48.00 Cartridge 4 sampling 663/1000ml at 0.25ml/s with 14.3psi vacuum @20:34:48.00 Cartridge 4 sampling 679/1000ml at 0.25ml/s with 13.8psi vacuum @20:35:47.99 Cartridge 4 sampling 695/1000ml at 0.25ml/s with 13.9psi vacuum @20:36:48.00 Cartridge 4 sampling 711/1000ml at 0.25ml/s with 15.1psi vacuum @20:37:48.00 Cartridge 4 sampling 727/1000ml at 0.26ml/s with 13.6psi vacuum @20:38:48.00 Cartridge 4 sampling 743/1000ml at 0.25ml/s with 13.7psi vacuum @20:39:48.00 Cartridge 4 sampling 758/1000ml at 0.25ml/s with 13.8psi vacuum @20:40:47.99 Cartridge 4 sampling 773/1000ml at 0.25ml/s with 13.0psi vacuum @20:41:48.00 Cartridge 4 sampling 789/1000ml at 0.25ml/s with 15.4psi vacuum @20:42:48.00 Cartridge 4 sampling 804/1000ml at 0.25ml/s with 13.0psi vacuum @20:43:48.00 Cartridge 4 sampling 819/1000ml at 0.25ml/s with 15.9psi vacuum @20:44:48.00 Cartridge 4 sampling 834/1000ml at 0.25ml/s with 14.7psi vacuum @20:45:47.99 Cartridge 4 sampling 849/1000ml at 0.25ml/s with 14.6psi vacuum @20:46:48.00 Cartridge 4 sampling 864/1000ml at 0.25ml/s with 12.8psi vacuum @20:47:13.25 Flow averaged 0.30ml/s over 48:23 @20:47:13.28 Waiting up to 7 seconds for vacuum to reduce from 14.5 to 10.0psi @20:47:13.98 Relieved excess vacuum in 5.9 seconds @20:47:14.00 SamplePump.seek 1000ml,9:09 @20:47:16.97 Flow estimate reduced to 0.24ml/s @20:47:16.99 Intake.close @20:47:17.04 Exhaust.close @20:47:17.21 Toroid.reconfigure :hold @20:47:17.28 Toroid.seek :bypass @20:47:18.90 Toroid.reconfigure :default @20:47:18.97 Toroid.seek :clear @20:47:20.59 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @20:47:20.61 Pressure.coast @20:47:20.79 Sampled 871.0ml @20:47:22.69 -> Cmd.startProcessing @20:47:24.54 SPR.command \"read_sequences\" @20:47:25.13 SPR.command \"stats_on\" @20:47:25.17 SPR.command \"start 0 1\" @20:47:27.17 Begun log /var/log/esp/spr19jul2820h.spr @20:47:28.20 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @20:47:29.88 Handoff.select :waste @20:47:30.06 Archive.seek :empty @20:47:37.23 Syringe tare is 21% of full scale @20:47:47.78 PinPort.seek :cartridge @20:47:52.34 Lysis1.seek 1350ul @20:47:54.81 Lysis2.seek 1700ul @20:47:57.91 Plunge.seek :reagent @20:47:58.76 Evacuating puck with filter bubble point of 30psi @20:47:58.77 PinPort.seek :cartridge @20:47:58.86 Handoff.select :waste @20:48:06.16 Air pumping 1 strokes to reach 4psi @20:48:06.18 Air.reconfigure :gulp @20:48:06.27 Air.seek :tVent @20:48:08.07 0.1psi after gulp @20:48:08.08 Air stroke #1 of 1 @20:48:08.10 Air.reconfigure :pressurize @20:48:08.19 Air.seek :pressurized @20:48:21.03 -0.1psi after pressurize @20:48:21.04 Air.reconfigure :compress @20:48:21.12 Air.seek :evac @20:48:23.78 0.1psi after compress @20:48:37.68 0.1psi after compress (stable) @20:48:37.80 at 0.1psi Opened to waste @20:48:37.81 Handoff.select :blocked @20:48:37.99 Air pumping 10 strokes to reach 30psi @20:48:38.01 Air.reconfigure :gulp @20:48:38.09 Air.seek :tVent @20:48:47.97 2.9psi after gulp @20:48:47.98 Air stroke #1 of 10 @20:48:48.00 Air.reconfigure :pressurize @20:48:48.07 Air.seek :pressurized @20:49:00.91 4.5psi after pressurize @20:49:00.92 Air.reconfigure :compress @20:49:01.00 Air.seek :evac @20:49:03.67 5.0psi after compress @20:50:01.25 28.9psi after compress (stable) @20:50:01.27 Air.reconfigure :gulp @20:50:01.35 Air.seek :tVent @20:50:03.17 Reached 30.0 of 30.0psi after pumping 1557ul (1.00 strokes) @20:50:03.18 Handoff.select :waste @20:50:11.48 delay 1:00 @20:51:11.50 Handoff.select :blocked @20:51:34.92 Pressure too High, open to waste again (4.1psi) @20:51:34.93 Handoff.select :waste @20:51:35.08 delay 1:00 @20:52:35.10 Handoff.select :blocked @20:53:00.07 Handoff.select :waste @20:53:00.19 PSI at @1.92psi at evacEnd @20:53:00.20 Handoff.select :blocked @20:53:00.32 Air.seek :tVent @20:53:16.10 Stable @2.1psi after Handoff blocked @20:53:16.19 Air pumping 10 strokes to reach 27psi @20:53:16.20 Air stroke #1 of 10 @20:53:16.22 Air.reconfigure :pressurize @20:53:16.30 Air.seek :pressurized @20:53:29.14 2.5psi after pressurize @20:53:29.15 Air.reconfigure :compress @20:53:29.23 Air.seek :evac @20:53:31.90 2.7psi after compress @20:54:40.89 20.2psi after compress (stable) @20:54:40.91 Air.reconfigure :gulp @20:54:40.98 Air.seek :tVent @20:54:50.88 22.3psi after gulp @20:54:50.89 Air stroke #2 of 10 @20:54:50.91 Air.reconfigure :pressurize @20:54:50.99 Air.seek :pressurized @20:55:03.82 20.8psi after pressurize @20:55:03.83 Air.reconfigure :compress @20:55:03.92 Air.seek :evac @20:55:06.58 19.9psi after compress @20:55:26.40 Reached 27.1 of 27.1psi after pumping 4672ul (3.00 strokes) @20:55:26.41 Handoff.select :waste @20:55:35.94 delay 1:00 @20:56:35.96 Handoff.select :blocked @20:56:43.90 Pressure too High, open to waste again (4.1psi) @20:56:43.91 Handoff.select :waste @20:56:44.03 delay 1:00 @20:57:44.05 Handoff.select :blocked @20:58:09.18 Handoff.select :waste @20:58:09.30 PSI at @2.66psi at evacEnd @20:58:09.31 Air.reconfigure :default @20:58:09.40 Air.seek :fVent @20:58:10.83 delay 5 seconds @20:58:15.85 Seawater evacuated, Presure above/below filter released. Lysis begins @20:58:15.92 Lysis1.reconfigure :slow @20:58:15.98 Lysis1.seek 1050ul @20:58:46.02 delay 5 seconds @20:58:51.04 Handoff.select :blocked @20:58:51.21 Lysis1.seek 450ul @20:59:51.19 delay 3 seconds @20:59:54.26 Air.reconfigure to mix into 1.37806 parts Lysis1 @20:59:54.73 Air.seek :evac @20:59:54.77 Lysis1.seek 300ul @21:00:09.88 Air.reconfigure :default @21:00:09.94 Air.seek :full @21:00:24.16 Lysis1.seek :empty @21:00:54.26 Lysis Delivered. Syringe pressure @57.7psi @21:00:54.32 PuckHeater.seek 85.6C @21:01:59.63 PuckHeater.hold 15:00,85.6C @21:16:58.96 Lysis complete. Syringe pressure @70.1psi @21:16:58.97 Handoff.select :blocked @21:16:59.09 Lysis1.reconfigure :halfSpeed @21:16:59.16 Lysis1.seek 200ul @21:17:02.93 delay 1 second @21:17:03.95 Lysis1.seek :empty @21:17:07.77 Initial AirS pressure @71.1psi @21:17:07.83 Lysis1.reconfigure :extract @21:17:07.93 Lysis1.seek 875ul,10:00 @21:17:09.64 Air pumping 10 strokes to reach 21psi @21:17:09.65 Air stroke #1 of 10 @21:17:09.67 Air.reconfigure :pressurize @21:17:09.75 Air.seek :pressurized @21:17:22.95 15.4psi after pressurize @21:17:22.97 Air.reconfigure :compress @21:17:23.05 Air.seek :evac @21:17:25.71 18.4psi after compress @21:17:39.64 15.8psi after compress (stable) @21:17:39.66 Air.reconfigure :gulp @21:17:39.75 Air.seek :tVent @21:17:49.65 16.1psi after gulp @21:17:49.66 Air stroke #2 of 10 @21:17:49.68 Air.reconfigure :pressurize @21:17:49.76 Air.seek :pressurized @21:18:02.60 15.3psi after pressurize @21:18:02.61 Air.reconfigure :compress @21:18:02.68 Air.seek :evac @21:18:05.35 17.0psi after compress @21:18:19.23 15.5psi after compress (stable) @21:18:19.25 Air.reconfigure :gulp @21:18:19.32 Air.seek :tVent @21:18:29.22 12.9psi after gulp @21:18:29.23 Air stroke #3 of 10 @21:18:29.24 Air.reconfigure :pressurize @21:18:29.32 Air.seek :pressurized @21:18:42.18 15.5psi after pressurize @21:18:42.20 Air.reconfigure :compress @21:18:42.28 Air.seek :evac @21:18:44.94 16.4psi after compress @21:18:59.23 Air.reconfigure :default @21:18:59.28 Air.hold @21:18:59.45 Lysis1 at 875ul pressure @16.1psi @21:18:59.46 Lysis1.reconfigure :default @21:18:59.54 Lysis1.seek 900ul @21:19:00.31 Lysis1 at 900ul pressure(finPSI) @12.4psi @21:19:00.32 delay 1 second @21:19:01.43 Lysis1 at 900ul pressure @13.7psi @21:19:01.45 Air.reconfigure :halfSpeed @21:19:01.52 Air.seek :fVent @21:19:03.77 Puck Top Vented. Syringe pressure @3.3psi @21:19:03.78 Handoff.select :waste @21:19:03.90 delay 5 seconds @21:19:08.92 Lysate Recovered in LyS1 @21:19:08.93 Lysis2.reconfigure :DAmix @21:19:09.04 Lysis1.reconfigure :DAmix @21:19:09.52 Lysis2.seek 1450ul @21:19:09.60 Lysis1.seek 650ul @21:19:44.65 delay 5 seconds @21:19:49.67 Handoff.select SPR @21:19:49.78 SPR.command \"start 2 14\" @21:19:50.83 delay 10 seconds @21:20:00.85 SPR.command \"start 1 0\" @21:20:04.55 Waiting up to 4:00 for SPR to enter state 1 @21:22:37.64 SPR.command \"hold_on\" @21:22:39.54 SPR Dilution 1 of 1 -- Running Experiment 1 @21:22:39.94 Lysis2.seek 1150ul @21:22:39.98 Lysis1.seek 350ul @21:23:22.06 volL1 = 300.0 and volL2 = 300.0 @21:23:22.09 delay 15 seconds @21:23:37.12 SPR.command \"hold_off\" @21:23:39.72 Waiting up to 25:00 for SPR to enter state 7 @21:43:52.62 SPR Analysis has Completed @21:43:52.64 Handoff.select :waste @21:43:53.56 Ended log /var/log/esp/spr19jul2820h.spr @21:43:53.57 SPR.command \"stats_off\" @21:43:55.23 -> Cmd.stop @21:43:55.32 Handoff.select :close @21:43:55.44 Gate.power :heat,:OFF @21:43:55.46 Gate.power :cartridge,:OFF @21:43:55.54 Carousel.seek 4 @21:43:55.71 Plunge.seek :locked @21:43:56.55 PinPort.seek :clear @21:44:01.16 Archive.seek :clear @21:44:01.29 Air.reconfigure :default @21:44:01.42 Lysis1.reconfigure :default @21:44:01.53 Air.seek :clear @21:44:01.59 Lysis1.seek :clear @21:44:01.67 Lysis2.reconfigure :default @21:44:01.75 Lysis2.seek :clear @21:44:18.92 Archive.coast @21:44:18.97 Air.coast @21:44:19.03 Lysis1.coast @21:44:19.08 Lysis2.coast @21:44:19.12 Plunge.coast @21:44:19.17 SamplePump.coast @21:44:19.22 Toroid.coast @21:44:19.62 Carousel.coast @21:44:19.67 PinPort.coast @21:44:19.85 Gate.power :motor,:OFF @21:44:19.87 Gate.power :core,:OFF @21:44:19.89 Gate.power :analytic1,:OFF @21:44:19.91 Gate.power :analytic2,:OFF @21:44:19.93 Gate.power :analytic3,:OFF @21:44:19.96 Gate.power :main,:OFF @21:44:19.98 Safely stopped and ready to power off ##-- file closed 2019-07-29T05:40:42.186Z ##-- file opened 2019-07-29T05:40:42.188Z @22:40:42.89 -> showStatus @22:40:44.90 -> Cmd.loadCartridge -9 @22:40:45.03 Gate.power :main,:ON,limit to 5000mA @22:40:45.04 Gate.power :analytic1,:ON,limit to 5000mA @22:40:45.06 Gate.power :analytic2,:ON,limit to 5000mA @22:40:45.08 Gate.power :analytic3,:ON,limit to 5000mA @22:40:45.22 Powering core bus @22:40:47.49 SPR Module 1, FW Rev. B, FPGA Rev. C @22:40:47.62 Archive.configure :default @22:40:47.74 Air.configure :default @22:40:47.91 Lysis1.configure :default @22:40:48.01 Lysis2.configure :default @22:40:48.13 Plunge.configure :default @22:40:48.25 SamplePump.configure :default @22:40:48.36 ExtraPump.configure :default @22:40:48.48 Toroid.configure :default @22:40:48.60 Carousel.configure :default @22:40:48.72 Pressure.configure :default @22:40:48.83 PinPort.configure :default @22:40:48.88 All elf controllers running firmware version 4.58 @22:40:48.91 Gate.power :motor,:ON,limit to 5000mA @22:40:49.10 Archive.reconfigure :default @22:40:49.16 Air.reconfigure :default @22:40:49.46 Air.home.jog 0 @22:40:49.51 Archive.home.jog 0 @22:40:49.64 Toroid.reconfigure :default @22:40:49.72 Plunge.reconfigure :default @22:40:49.75 Lysis2.reconfigure :default @22:40:49.81 PinPort.reconfigure :default @22:40:49.86 Lysis1.reconfigure :default @22:40:50.09 PinPort.home.jog 0 @22:40:50.14 Toroid.home.jog 0 @22:40:50.19 Lysis2.home.jog 0 @22:40:50.25 Plunge.home.jog 0 @22:40:50.31 Lysis1.home.jog 0 @22:40:50.48 Archive.reconfigure :homing @22:40:50.56 Air.reconfigure :homing @22:40:50.80 Archive.jog 1411 @22:40:50.86 Air.jog 150 @22:40:50.98 PinPort.reconfigure :homing @22:40:51.05 Toroid.reconfigure :homing @22:40:51.08 Lysis2.reconfigure :homing @22:40:51.12 Lysis1.reconfigure :homing @22:40:51.15 Plunge.reconfigure :homing @22:40:51.37 Plunge.jog 1552 @22:40:51.42 Toroid.jog 624 @22:40:51.48 Lysis2.jog 159 @22:40:51.53 Lysis1.jog 1411 @22:40:51.59 PinPort.jog -106 @22:40:51.67 Air.setRawPosition! 50 @22:40:51.72 Air.seek :home @22:40:51.82 PinPort.setRawPosition! 0 @22:40:51.86 PinPort.seek :home @22:40:52.00 PinPort.reconfigure :default @22:40:52.03 Air.reconfigure :default @22:40:52.06 Archive.setRawPosition! 50 @22:40:52.09 Lysis2.setRawPosition! 50 @22:40:52.25 Archive.seek :home @22:40:52.28 Lysis2.seek :home @22:40:52.61 Archive.reconfigure :default @22:40:52.69 Plunge.setRawPosition! 50 @22:40:52.72 Lysis2.reconfigure :default @22:40:52.74 Lysis1.setRawPosition! 50 @22:40:52.85 Plunge.seek :home @22:40:52.89 Lysis1.seek :home @22:40:53.17 Plunge.reconfigure :default @22:40:53.25 Lysis1.reconfigure :default @22:40:53.54 Toroid.setRawPosition! 0 @22:40:53.58 Toroid.seek :home @22:40:53.67 Toroid.reconfigure :default @22:40:53.80 Toroid.seek :clear @22:40:55.43 Carousel.reconfigure :default @22:40:55.49 Carousel.home.jog 0 @22:40:55.99 Carousel.seek 4 @22:40:56.13 Selecting Cartridge 3 @22:40:56.38 Carousel.seek 3 @22:40:56.86 Lysis1.seek :locked @22:41:01.00 Plunge.seek :locked @22:41:01.12 Air.seek :locked @22:41:05.25 Powering cartridge bus @22:41:07.37 Gate.setIR 1750,5000,0,6 @22:41:07.50 Gate.setIR 0,5000,0,6 @22:41:07.55 Loaded Lyse-n-Go Cartridge @22:41:07.56 PuckHeater.configure HeatConfig @22:41:07.61 Gate.power :heat,:ON,limit to 5000mA @22:41:23.95 -> Cmd.startFiltering @22:41:24.18 Duration of filtering limited to 2:05:00 @22:41:24.43 Priming sample loop w/100ml, bypass w/10ml @22:41:24.44 SamplePump.setPosition! 0ml @22:41:24.48 Bypass.open @22:41:25.93 Intake.open @22:41:26.21 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @22:41:29.42 Vacuum tare is 52% of full scale @22:41:29.54 Pressure.reconfigure :default @22:41:29.60 Pressure.hold @22:41:29.77 SamplePump.reconfigure :prime @22:41:29.84 SamplePump.seek 10ml,1:10 @22:41:38.80 Pressure.coast @22:41:38.85 SamplePump.setPosition! 0ml @22:41:38.90 Exhaust.open @22:41:40.31 Bypass.close @22:41:41.85 Pressure.reconfigure :default @22:41:41.91 Pressure.hold @22:41:42.14 SamplePump.reconfigure :prime @22:41:42.21 SamplePump.seek 100ml,7:10 @22:43:37.92 Pressure.coast @22:43:37.98 Bypass.open @22:43:38.72 Intake.close @22:43:39.56 Toroid.reconfigure :hold @22:43:39.62 Toroid.seek :crack @22:43:40.42 Toroid.reconfigure :default @22:43:40.48 Toroid.seek :clear @22:43:42.04 Toroid.reconfigure :hold @22:43:42.11 Toroid.seek :cartridge @22:43:43.92 Toroid.reconfigure :default @22:43:43.98 Toroid.seek :clear @22:43:45.96 Pressurizing cartridge to 14psi above 19.7psia @22:43:45.97 SamplePump.setPosition! 0ml @22:43:46.01 Intake.open @22:43:46.77 Bypass.close @22:43:47.47 Exhaust.close @22:43:48.28 Pressure.reconfigure :default @22:43:48.33 Pressure.hold @22:43:48.50 SamplePump.reconfigure :charge @22:43:48.57 SamplePump.seek 2.3ml,41 seconds @22:43:55.83 Pressure.coast @22:43:55.89 Intake.close @22:43:56.68 Pressurized cartridge with 0.245ml to 14.1psi @22:43:56.69 Isolated @32.0psia (12.3psi) @22:43:56.70 Checking for cartridge leaks from 32.0psia @22:43:56.73 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @22:44:01.82 Sampling 1000.0ml @22:44:01.84 SamplePump.setPosition! 0ml @22:44:01.89 Exhaust.open @22:44:02.63 Intake.open @22:44:03.45 Pressure.reconfigure :default @22:44:03.51 Pressure.hold @22:44:03.63 Sea 3.3m deep (19.6psia) @22:44:03.76 SamplePump.reconfigure :sample @22:44:03.84 SamplePump.seek 1000ml,1:07:09 @22:44:06.49 Cartridge 3 sampling 1/1000ml at 0.33ml/s with 11.4psi vacuum 1.7m deep (17.2psia) @22:45:06.49 Cartridge 3 sampling 22/1000ml at 0.34ml/s with 13.5psi vacuum @22:46:06.49 Cartridge 3 sampling 43/1000ml at 0.34ml/s with 13.0psi vacuum @22:47:06.49 Cartridge 3 sampling 64/1000ml at 0.35ml/s with 13.0psi vacuum @22:48:06.49 Cartridge 3 sampling 85/1000ml at 0.33ml/s with 12.4psi vacuum @22:49:06.49 Cartridge 3 sampling 105/1000ml at 0.34ml/s with 13.5psi vacuum @22:50:06.49 Cartridge 3 sampling 125/1000ml at 0.33ml/s with 14.7psi vacuum @22:51:06.49 Cartridge 3 sampling 146/1000ml at 0.35ml/s with 12.7psi vacuum @22:52:06.49 Cartridge 3 sampling 166/1000ml at 0.31ml/s with 12.7psi vacuum @22:53:06.49 Cartridge 3 sampling 186/1000ml at 0.34ml/s with 12.7psi vacuum @22:54:06.49 Cartridge 3 sampling 205/1000ml at 0.32ml/s with 12.4psi vacuum @22:55:06.49 Cartridge 3 sampling 225/1000ml at 0.33ml/s with 11.6psi vacuum @22:56:06.49 Cartridge 3 sampling 244/1000ml at 0.32ml/s with 14.4psi vacuum @22:57:06.49 Cartridge 3 sampling 263/1000ml at 0.32ml/s with 13.4psi vacuum @22:58:06.49 Cartridge 3 sampling 282/1000ml at 0.30ml/s with 13.7psi vacuum @22:59:06.49 Cartridge 3 sampling 301/1000ml at 0.30ml/s with 13.8psi vacuum @23:00:06.49 Cartridge 3 sampling 320/1000ml at 0.30ml/s with 12.5psi vacuum @23:01:06.49 Cartridge 3 sampling 338/1000ml at 0.32ml/s with 12.0psi vacuum 1.6m deep (17.1psia) @23:02:06.49 Cartridge 3 sampling 356/1000ml at 0.28ml/s with 12.9psi vacuum @23:03:06.49 Cartridge 3 sampling 374/1000ml at 0.28ml/s with 13.2psi vacuum @23:04:06.49 Cartridge 3 sampling 392/1000ml at 0.32ml/s with 13.3psi vacuum @23:05:06.49 Cartridge 3 sampling 409/1000ml at 0.28ml/s with 12.9psi vacuum @23:06:06.49 Cartridge 3 sampling 426/1000ml at 0.27ml/s with 13.1psi vacuum @23:07:06.49 Cartridge 3 sampling 443/1000ml at 0.28ml/s with 13.2psi vacuum @23:08:06.49 Cartridge 3 sampling 460/1000ml at 0.29ml/s with 14.5psi vacuum @23:09:06.49 Cartridge 3 sampling 476/1000ml at 0.30ml/s with 13.1psi vacuum @23:10:06.49 Cartridge 3 sampling 493/1000ml at 0.29ml/s with 14.0psi vacuum @23:11:06.86 Cartridge 3 sampling 509/1000ml at 0.25ml/s with 13.0psi vacuum @23:12:06.49 Cartridge 3 sampling 525/1000ml at 0.25ml/s with 13.0psi vacuum @23:13:06.49 Cartridge 3 sampling 541/1000ml at 0.28ml/s with 14.0psi vacuum @23:14:06.49 Cartridge 3 sampling 556/1000ml at 0.27ml/s with 12.5psi vacuum @23:15:06.49 Cartridge 3 sampling 572/1000ml at 0.25ml/s with 15.1psi vacuum @23:16:06.49 Cartridge 3 sampling 587/1000ml at 0.25ml/s with 13.9psi vacuum @23:17:06.49 Cartridge 3 sampling 602/1000ml at 0.25ml/s with 14.3psi vacuum @23:18:06.49 Cartridge 3 sampling 617/1000ml at 0.26ml/s with 13.4psi vacuum @23:19:06.49 Cartridge 3 sampling 633/1000ml at 0.25ml/s with 13.5psi vacuum @23:20:06.49 Cartridge 3 sampling 648/1000ml at 0.25ml/s with 15.4psi vacuum @23:20:37.58 Flow averaged 0.30ml/s over 36:33 @23:20:37.61 Waiting up to 7 seconds for vacuum to reduce from 14.8 to 10.0psi @23:20:38.62 Relieved excess vacuum in 2 seconds @23:20:38.64 SamplePump.seek 1000ml,23:29 @23:21:06.49 Cartridge 3 sampling 662/1000ml at 0.25ml/s with 15.0psi vacuum @23:21:31.07 Flow averaged 0.27ml/s over 48 seconds @23:21:31.10 Waiting up to 7 seconds for vacuum to reduce from 14.5 to 10.0psi @23:21:32.10 Relieved excess vacuum in 5.5 seconds @23:21:32.12 SamplePump.seek 1000ml,22:36 @23:21:34.34 Flow estimate reduced to 0.22ml/s @23:21:34.36 Intake.close @23:21:34.40 Exhaust.close @23:21:34.58 Toroid.reconfigure :hold @23:21:34.65 Toroid.seek :bypass @23:21:36.28 Toroid.reconfigure :default @23:21:36.35 Toroid.seek :clear @23:21:37.97 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @23:21:37.99 Pressure.coast @23:21:38.17 Sampled 669.0ml @23:21:40.22 -> Cmd.startProcessing @23:21:42.07 SPR.command \"read_sequences\" @23:21:42.77 SPR.command \"stats_on\" @23:21:42.81 SPR.command \"start 0 1\" @23:21:44.81 Begun log /var/log/esp/spr19jul2823h.spr @23:21:45.62 Handoff.select :waste @23:21:45.80 Archive.seek :empty @23:21:52.96 Syringe tare is 21% of full scale @23:22:03.54 PinPort.seek :cartridge @23:22:08.10 Lysis1.seek 1350ul @23:22:10.58 Lysis2.seek 1700ul @23:22:13.68 Plunge.seek :reagent @23:22:14.52 Evacuating puck with filter bubble point of 30psi @23:22:14.53 PinPort.seek :cartridge @23:22:14.62 Handoff.select :waste @23:22:21.94 Air pumping 1 strokes to reach 4psi @23:22:21.96 Air.reconfigure :gulp @23:22:22.05 Air.seek :tVent @23:22:23.84 0.0psi after gulp @23:22:23.85 Air stroke #1 of 1 @23:22:23.88 Air.reconfigure :pressurize @23:22:23.96 Air.seek :pressurized @23:22:36.80 0.1psi after pressurize @23:22:36.81 Air.reconfigure :compress @23:22:36.89 Air.seek :evac @23:22:39.55 0.6psi after compress @23:22:53.43 0.4psi after compress (stable) @23:22:53.55 at 0.4psi Opened to waste @23:22:53.56 Handoff.select :blocked @23:22:54.11 Air pumping 10 strokes to reach 30psi @23:22:54.13 Air.reconfigure :gulp @23:22:54.23 Air.seek :tVent @23:23:04.12 9.4psi after gulp @23:23:04.13 Air stroke #1 of 10 @23:23:04.15 Air.reconfigure :pressurize @23:23:04.23 Air.seek :pressurized @23:23:17.07 9.8psi after pressurize @23:23:17.08 Air.reconfigure :compress @23:23:17.16 Air.seek :evac @23:23:19.83 15.8psi after compress @23:23:37.01 23.8psi after compress (stable) @23:23:37.03 Air.reconfigure :gulp @23:23:37.11 Air.seek :tVent @23:23:47.00 24.0psi after gulp @23:23:47.01 Air stroke #2 of 10 @23:23:47.02 Air.reconfigure :pressurize @23:23:47.11 Air.seek :pressurized @23:23:59.96 23.7psi after pressurize @23:23:59.97 Air.reconfigure :compress @23:24:00.04 Air.seek :evac @23:24:02.72 29.8psi after compress @23:24:02.88 Reached 30.6 of 30.0psi after pumping 4672ul (3.00 strokes) @23:24:02.89 Handoff.select :waste @23:24:25.73 delay 1:00 @23:25:25.75 Handoff.select :blocked @23:25:51.16 Handoff.select :waste @23:25:51.28 PSI at @0.98psi at evacEnd @23:25:51.29 Handoff.select :blocked @23:25:51.43 Air.reconfigure :gulp @23:25:51.51 Air.seek :tVent @23:26:08.57 Stable @0.9psi after Handoff blocked @23:26:08.65 Air pumping 10 strokes to reach 26psi @23:26:08.67 Air stroke #1 of 10 @23:26:08.69 Air.reconfigure :pressurize @23:26:08.77 Air.seek :pressurized @23:26:21.61 1.2psi after pressurize @23:26:21.62 Air.reconfigure :compress @23:26:21.70 Air.seek :evac @23:26:24.36 3.3psi after compress @23:26:46.02 11.1psi after compress (stable) @23:26:46.04 Air.reconfigure :gulp @23:26:46.12 Air.seek :tVent @23:26:56.00 11.9psi after gulp @23:26:56.01 Air stroke #2 of 10 @23:26:56.03 Air.reconfigure :pressurize @23:26:56.11 Air.seek :pressurized @23:27:08.95 12.0psi after pressurize @23:27:08.96 Air.reconfigure :compress @23:27:09.04 Air.seek :evac @23:27:11.70 13.6psi after compress @23:27:34.30 20.9psi after compress (stable) @23:27:34.32 Air.reconfigure :gulp @23:27:34.40 Air.seek :tVent @23:27:44.29 21.6psi after gulp @23:27:44.30 Air stroke #3 of 10 @23:27:44.32 Air.reconfigure :pressurize @23:27:44.40 Air.seek :pressurized @23:27:57.23 21.8psi after pressurize @23:27:57.24 Air.reconfigure :compress @23:27:57.33 Air.seek :evac @23:28:00.00 22.7psi after compress @23:28:02.29 Reached 26.0 of 25.9psi after pumping 6230ul (4.00 strokes) @23:28:02.31 Handoff.select :waste @23:28:11.85 delay 1:00 @23:29:11.87 Handoff.select :blocked @23:29:36.85 Handoff.select :waste @23:29:36.97 PSI at @0.80psi at evacEnd @23:29:36.98 Air.reconfigure :default @23:29:37.07 Air.seek :fVent @23:29:38.53 delay 5 seconds @23:29:43.55 Seawater evacuated, Presure above/below filter released. Lysis begins @23:29:43.62 Lysis1.reconfigure :slow @23:29:43.69 Lysis1.seek 1050ul @23:30:13.74 delay 5 seconds @23:30:18.76 Handoff.select :blocked @23:30:18.92 Lysis1.seek 450ul @23:31:18.89 delay 3 seconds @23:31:21.96 Air.reconfigure to mix into 1.37806 parts Lysis1 @23:31:22.36 Air.seek :evac @23:31:22.40 Lysis1.seek 300ul @23:31:37.53 Air.reconfigure :default @23:31:37.59 Air.seek :full @23:31:51.81 Lysis1.seek :empty @23:32:21.92 Lysis Delivered. Syringe pressure @50.3psi @23:32:21.97 PuckHeater.seek 85.6C @23:33:23.14 PuckHeater.hold 15:00,85.6C @23:48:22.27 Lysis complete. Syringe pressure @52.6psi @23:48:22.28 Handoff.select :blocked @23:48:22.38 Lysis1.reconfigure :halfSpeed @23:48:22.45 Lysis1.seek 200ul @23:48:26.21 delay 1 second @23:48:27.23 Lysis1.seek :empty @23:48:31.05 Initial AirS pressure @52.6psi @23:48:31.11 Lysis1.reconfigure :extract @23:48:31.23 Lysis1.seek 875ul,10:00 @23:48:32.92 Air pumping 10 strokes to reach 21psi @23:48:32.93 Air stroke #1 of 10 @23:48:32.95 Air.reconfigure :pressurize @23:48:33.04 Air.seek :pressurized @23:48:45.88 16.0psi after pressurize @23:48:45.90 Air.reconfigure :compress @23:48:45.97 Air.seek :evac @23:48:48.64 16.5psi after compress @23:48:49.86 Reached 30.2 of 21.0psi after pumping 3115ul (2.00 strokes) @23:48:50.54 Air pumping 8.0 strokes to reach 21psi @23:48:50.56 Air.reconfigure :gulp @23:48:50.64 Air.seek :tVent @23:49:00.54 16.9psi after gulp @23:49:00.55 Air stroke #1 of 8.0 @23:49:00.57 Air.reconfigure :pressurize @23:49:00.65 Air.seek :pressurized @23:49:13.48 15.2psi after pressurize @23:49:13.49 Air.reconfigure :compress @23:49:13.58 Air.seek :evac @23:49:16.24 16.6psi after compress @23:49:21.47 Air.reconfigure :default @23:49:21.52 Air.hold @23:49:21.68 Lysis1 at 875ul pressure @16.7psi @23:49:21.70 Lysis1.reconfigure :default @23:49:21.78 Lysis1.seek 900ul @23:49:22.54 Lysis1 at 900ul pressure(finPSI) @14.8psi @23:49:22.55 delay 1 second @23:49:23.68 Lysis1 at 900ul pressure @17.7psi @23:49:23.70 Air.reconfigure :halfSpeed @23:49:23.78 Air.seek :fVent @23:49:26.05 Puck Top Vented. Syringe pressure @1.4psi @23:49:26.06 Handoff.select :waste @23:49:26.17 delay 5 seconds @23:49:31.19 Lysate Recovered in LyS1 @23:49:31.21 Lysis2.reconfigure :DAmix @23:49:31.31 Lysis1.reconfigure :DAmix @23:49:31.71 Lysis2.seek 1450ul @23:49:31.78 Lysis1.seek 650ul @23:50:06.82 delay 5 seconds @23:50:11.84 Handoff.select SPR @23:50:11.95 SPR.command \"start 2 14\" @23:50:12.95 delay 10 seconds @23:50:22.97 SPR.command \"start 1 0\" @23:50:26.20 Waiting up to 4:00 for SPR to enter state 1 @23:52:58.82 SPR.command \"hold_on\" @23:53:00.26 SPR Dilution 1 of 1 -- Running Experiment 1 @23:53:00.67 Lysis2.seek 1150ul @23:53:00.73 Lysis1.seek 350ul @23:53:42.75 volL1 = 300.0 and volL2 = 300.0 @23:53:42.77 delay 15 seconds @23:53:57.80 SPR.command \"hold_off\" @23:54:00.90 Waiting up to 25:00 for SPR to enter state 7 @00:14:14.75PDT29-Jul-19 SPR Analysis has Completed @00:14:14.76 Handoff.select :waste @00:14:15.68 Ended log /var/log/esp/spr19jul2823h.spr @00:14:15.69 SPR.command \"stats_off\" @00:14:17.19 -> Cmd.stop @00:14:17.28 Handoff.select :close @00:14:17.40 Gate.power :heat,:OFF @00:14:17.44 Gate.power :cartridge,:OFF @00:14:17.54 Carousel.seek 3 @00:14:17.72 Plunge.seek :locked @00:14:18.56 PinPort.seek :clear @00:14:23.17 Archive.seek :clear @00:14:23.30 Air.reconfigure :default @00:14:23.43 Lysis1.reconfigure :default @00:14:23.53 Air.seek :clear @00:14:23.59 Lysis1.seek :clear @00:14:23.67 Lysis2.reconfigure :default @00:14:23.75 Lysis2.seek :clear @00:14:40.93 Archive.coast @00:14:40.99 Air.coast @00:14:41.03 Lysis1.coast @00:14:41.08 Lysis2.coast @00:14:41.13 Plunge.coast @00:14:41.51 SamplePump.coast @00:14:41.58 Toroid.coast @00:14:41.63 Carousel.coast @00:14:41.68 PinPort.coast @00:14:41.86 Gate.power :motor,:OFF @00:14:41.88 Gate.power :core,:OFF @00:14:41.90 Gate.power :analytic1,:OFF @00:14:41.92 Gate.power :analytic2,:OFF @00:14:41.94 Gate.power :analytic3,:OFF @00:14:41.96 Gate.power :main,:OFF @00:14:41.98 Safely stopped and ready to power off ##-- file closed 2019-07-29T18:23:24.333Z