##-- file opened 2023-04-25T20:17:39.709Z
@13:17:40.87 -> showStatus
@13:17:42.49 -> Cmd.loadCartridge -6
@13:17:44.52 -> Cmd.stop
@13:17:44.53 ready to power off
##-- file closed 2023-04-25T20:54:49.302Z

##-- file opened 2023-04-25T20:54:49.309Z
@13:54:49.29 -> showStatus
@13:54:51.31 -> Cmd.loadCartridge -10
@13:54:51.37 Gate.power :main,:ON,limit to 5000mA
@13:54:51.39 Gate.power :analytic1,:ON,limit to 5000mA
@13:54:51.41 Gate.power :analytic2,:ON,limit to 5000mA
@13:54:51.44 Gate.power :analytic3,:ON,limit to 5000mA
@13:54:51.57 Powering core bus
@13:54:53.85 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@13:54:54.27 <LRAUV> Archive.configure :default
@13:54:54.36 Air.configure :default
@13:54:54.42 Lysis1.configure :default
@13:54:54.49 Lysis2.configure :default
@13:54:54.58 Plunge.configure :default
@13:54:54.67 SamplePump.configure :default
@13:54:54.76 Toroid.configure :default
@13:54:54.85 Carousel.configure :default
@13:54:54.94 Pressure.configure :default
@13:54:55.03 ExtraPump.configure :default
@13:54:55.10 PinPort.configure :default
@13:54:55.17 All elf controllers running firmware version 4.83
@13:54:55.20 Gate.power :motor,:ON,limit to 5000mA
@13:54:55.47 <Archive_home> Archive.home.jog 0
@13:54:55.92 <Carousel_home> Carousel.home.jog 0
@13:54:55.98 <Toroid_home> Toroid.home.jog 0
@13:54:56.00 <Lysis1_home> Lysis1.home.jog 0
@13:54:56.05 <Lysis2_home> Lysis2.home.jog 0
@13:54:56.10 <PinPort_home> PinPort.home.jog 0
@13:54:56.14 <Plunge_home> Plunge.home.jog 0
@13:54:56.18 <Air_home> Air.home.jog 0
@13:54:56.70 <Archive_home> Archive.in :homing
@13:54:57.00 <Toroid_home> Toroid.in :homing
@13:54:57.04 <Plunge_home> Plunge.in :homing
@13:54:57.07 <Archive_home> Archive.jog 1380
@13:54:57.11 <Lysis2_home> Lysis2.in :homing
@13:54:57.15 <Air_home> Air.in :homing
@13:54:57.18 <PinPort_home> PinPort.in :homing
@13:54:57.22 <Lysis1_home> Lysis1.in :homing
@13:54:57.40 <Toroid_home> Toroid.jog 626
@13:54:57.45 <Plunge_home> Plunge.jog 1538
@13:54:57.51 <Air_home> Air.jog 1372
@13:54:57.56 <PinPort_home> PinPort.jog -105
@13:54:57.61 <Lysis2_home> Lysis2.jog 1397
@13:54:57.67 <Lysis1_home> Lysis1.jog 1398
@13:54:58.26 <PinPort_home> PinPort.setRawPosition! 0
@13:54:58.31 PinPort.seek :home
@13:54:58.40 PinPort.in :default
@13:54:58.48 <Archive_home> Archive.setRawPosition! 50
@13:54:58.53 Archive.seek :home
@13:54:58.81 Archive.in :default
@13:54:58.91 <Plunge_home> Plunge.setRawPosition! 50
@13:54:58.93 <Air_home> Air.setRawPosition! 50
@13:54:59.01 <Plunge_home> Plunge.seek :home
@13:54:59.07 <Air_home> Air.seek :home
@13:54:59.27 <Lysis2_home> Lysis2.setRawPosition! 50
@13:54:59.33 Lysis2.seek :home
@13:54:59.44 <Plunge_home> Plunge.in :default
@13:54:59.45 <Lysis1_home> Lysis1.setRawPosition! 50
@13:54:59.48 <Air_home> Air.in :default
@13:54:59.61 <Lysis1_home> Lysis1.seek :home
@13:54:59.73 <Toroid_home> Toroid.setRawPosition! 0
@13:54:59.76 <Lysis2_home> Lysis2.in :default
@13:54:59.85 <Toroid_home> Toroid.seek :home
@13:54:59.97 <Lysis1_home> Lysis1.in :default
@13:55:00.01 <Toroid_home> Toroid.in :default
@13:55:00.36 <LRAUV> Toroid.seek :clear
@13:55:01.98 Carousel.in :default
@13:55:02.10 Carousel.home.seek 54
@13:55:02.41 Selecting Cartridge 9
@13:55:02.69 Carousel.seek 9
@13:55:12.15 Lysis1.in :fast
@13:55:12.22 Lysis1.seek :locked
@13:55:14.81 <Plunge> Plunge.seek :locked
@13:55:14.95 <Air> Air.in :fast
@13:55:15.02 Air.seek :locked
@13:55:16.59 <LRAUV> Carousel.reconfigure :locked
@13:55:16.79 Powering cartridge bus
@13:55:19.41 Gate.setIR 1750,5000,0,6
@13:55:19.53 Gate.setIR 0,5000,0,6
@13:55:19.60 PuckHeater.configure :default
@13:55:19.66 Gate.power :heat,:ON,limit to 5000mA
@13:55:20.34 Mounted lysis Cartridge board#Q05045
@13:55:20.38 Using default cartridge heat loss curve for HeatingAccelInCarousel
@13:55:28.88 -> Cmd.startFiltering
@13:55:29.13 <FILTERING> Preparing to filter cartridge 9
@13:55:29.42 Priming sample loop w/100ml, bypass w/10ml
@13:55:29.43 SamplePump.setPosition! 0ml
@13:55:29.49 Bypass.open
@13:55:30.95 Intake.open
@13:55:31.69 Sea 6.9m deep (24.9psia)
@13:55:31.71 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@13:55:34.29 Vacuum tare is 60% of full scale
@13:55:34.79 Pressure.in :default
@13:55:34.85 Pressure.hold
@13:55:35.04 SamplePump.in :prime
@13:55:35.12 SamplePump.seek 10ml,59 seconds
@13:55:43.86 Pressure.stop
@13:55:43.92 SamplePump.setPosition! 0ml
@13:55:43.98 Exhaust.open
@13:55:45.43 <closeBypass> Bypass.close
@13:55:46.93 <FILTERING> Pressure.in :default
@13:55:46.99 Pressure.hold
@13:55:47.12 Sea 7.0m deep (25.1psia)
@13:55:47.27 SamplePump.in :prime
@13:55:47.34 SamplePump.seek 100ml,5:16
@13:57:24.35 Pressure.stop
@13:57:24.43 Bypass.open
@13:57:25.16 Intake.close
@13:57:25.91 Toroid.in :hold
@13:57:25.98 Toroid.seek :crack
@13:57:27.51 Toroid.seek :cartridge
@13:57:28.61 Toroid.in :default
@13:57:28.67 Toroid.seek :clear
@13:57:30.65 Pressurizing cartridge to 26psi above 23.9psia
@13:57:30.66 SamplePump.setPosition! 0ml
@13:57:30.71 Intake.open
@13:57:31.37 Bypass.close
@13:57:32.07 Exhaust.close
@13:57:32.85 Pressure.in :default
@13:57:32.91 Pressure.hold
@13:57:33.09 SamplePump.in :charge
@13:57:33.17 SamplePump.seek 2.3ml,42 seconds
@13:57:43.44 Pressure.stop
@13:57:43.51 Intake.close
@13:57:44.25 Pressurized cartridge with 0.501ml to 26.1psi
@13:57:44.26 Isolated @49.2psia (25.3psi)
@13:57:44.28 Verifying cartridge holds at least 0.65..0.85 of 49.2psia
@13:57:44.31 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@13:57:48.97 Sampling 1000.0ml in 1:25:20
@13:57:48.99 SamplePump.setPosition! 0ml
@13:57:49.06 Exhaust.open
@13:57:49.77 Intake.open
@13:57:50.57 Pressure.in :default
@13:57:50.64 Pressure.hold
@13:57:51.14 Sea 7.5m deep (25.8psia)
@13:57:51.15 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s
@13:57:51.31 SamplePump.in :sample
@13:57:51.39 SamplePump.seek 1000ml,1:23:51
@13:57:53.66 <sampling> Cartridge 9 filtering 1/1000ml at 0.39ml/s with 24.3psi vacuum 6.9m deep (24.9psia)
@13:58:53.61 Cartridge 9 filtering 23/1000ml at 0.37ml/s with 23.9psi vacuum 6.1m deep (23.8psia)
@13:59:53.62 Cartridge 9 filtering 45/1000ml at 0.35ml/s with 22.4psi vacuum 5.6m deep (23.0psia)
@14:00:53.62 Cartridge 9 filtering 66/1000ml at 0.36ml/s with 23.2psi vacuum 7.8m deep (26.3psia)
@14:01:53.62 Cartridge 9 filtering 86/1000ml at 0.34ml/s with 21.9psi vacuum 6.6m deep (24.4psia)
@14:02:53.62 Cartridge 9 filtering 107/1000ml at 0.31ml/s with 24.7psi vacuum 6.8m deep (24.8psia)
@14:03:53.61 Cartridge 9 filtering 127/1000ml at 0.31ml/s with 26.4psi vacuum 4.6m deep (21.5psia)
@14:04:53.62 Cartridge 9 filtering 146/1000ml at 0.35ml/s with 24.4psi vacuum 6.5m deep (24.2psia)
@14:05:53.62 Cartridge 9 filtering 166/1000ml at 0.31ml/s with 21.2psi vacuum 7.4m deep (25.6psia)
@14:06:53.62 Cartridge 9 filtering 185/1000ml at 0.30ml/s with 24.9psi vacuum 5.4m deep (22.6psia)
@14:07:53.62 Cartridge 9 filtering 203/1000ml at 0.29ml/s with 25.5psi vacuum 4.7m deep (21.6psia)
@14:08:53.62 Cartridge 9 filtering 222/1000ml at 0.31ml/s with 24.9psi vacuum 6.6m deep (24.4psia)
@14:09:53.62 Cartridge 9 filtering 240/1000ml at 0.31ml/s with 25.4psi vacuum 6.7m deep (24.6psia)
@14:10:53.62 Cartridge 9 filtering 258/1000ml at 0.30ml/s with 24.9psi vacuum 5.4m deep (22.7psia)
@14:11:53.62 Cartridge 9 filtering 275/1000ml at 0.28ml/s with 23.9psi vacuum 7.4m deep (25.6psia)
@14:12:53.62 Cartridge 9 filtering 293/1000ml at 0.31ml/s with 23.3psi vacuum 6.3m deep (24.1psia)
@14:13:53.62 Cartridge 9 filtering 309/1000ml at 0.27ml/s with 26.3psi vacuum 5.2m deep (22.4psia)
@14:14:53.62 Cartridge 9 filtering 326/1000ml at 0.29ml/s with 24.2psi vacuum 6.8m deep (24.8psia)
@14:15:53.62 Cartridge 9 filtering 343/1000ml at 0.29ml/s with 22.9psi vacuum 6.6m deep (24.4psia)
@14:16:53.62 Cartridge 9 filtering 359/1000ml at 0.28ml/s with 25.7psi vacuum 5.4m deep (22.6psia)
@14:17:53.62 Cartridge 9 filtering 375/1000ml at 0.26ml/s with 24.0psi vacuum 6.4m deep (24.2psia)
@14:18:53.62 Cartridge 9 filtering 390/1000ml at 0.24ml/s with 24.1psi vacuum 5.5m deep (22.8psia)
@14:19:53.62 Cartridge 9 filtering 406/1000ml at 0.27ml/s with 24.2psi vacuum 6.7m deep (24.6psia)
@14:20:53.62 Cartridge 9 filtering 421/1000ml at 0.26ml/s with 23.3psi vacuum 6.0m deep (23.6psia)
@14:21:53.62 Cartridge 9 filtering 436/1000ml at 0.26ml/s with 25.4psi vacuum 5.2m deep (22.4psia)
@14:22:53.62 Cartridge 9 filtering 451/1000ml at 0.22ml/s with 24.1psi vacuum 6.0m deep (23.5psia)
@14:23:53.62 Cartridge 9 filtering 465/1000ml at 0.24ml/s with 25.4psi vacuum 5.4m deep (22.6psia)
@14:24:53.62 Cartridge 9 filtering 479/1000ml at 0.23ml/s with 25.5psi vacuum 6.1m deep (23.8psia)
@14:25:53.62 Cartridge 9 filtering 493/1000ml at 0.22ml/s with 22.7psi vacuum 7.0m deep (25.1psia)
@14:26:53.62 Cartridge 9 filtering 507/1000ml at 0.23ml/s with 26.2psi vacuum 6.1m deep (23.7psia)
@14:27:53.62 Cartridge 9 filtering 521/1000ml at 0.22ml/s with 25.3psi vacuum 6.1m deep (23.8psia)
@14:28:53.62 Cartridge 9 filtering 534/1000ml at 0.22ml/s with 22.6psi vacuum 5.7m deep (23.1psia)
@14:29:53.62 Cartridge 9 filtering 547/1000ml at 0.20ml/s with 24.9psi vacuum 5.9m deep (23.4psia)
@14:30:53.62 Cartridge 9 filtering 560/1000ml at 0.20ml/s with 23.8psi vacuum 4.7m deep (21.7psia)
@14:31:54.04 Cartridge 9 filtering 573/1000ml at 0.21ml/s with 24.4psi vacuum 5.6m deep (22.9psia)
@14:32:53.62 Cartridge 9 filtering 586/1000ml at 0.21ml/s with 24.9psi vacuum 5.5m deep (22.9psia)
@14:33:53.67 Cartridge 9 filtering 598/1000ml at 0.20ml/s with 25.8psi vacuum 4.7m deep (21.6psia)
@14:34:53.62 Cartridge 9 filtering 611/1000ml at 0.20ml/s with 24.1psi vacuum 5.1m deep (22.2psia)
@14:35:53.62 Cartridge 9 filtering 623/1000ml at 0.20ml/s with 24.9psi vacuum 4.8m deep (21.8psia)
@14:36:53.62 Cartridge 9 filtering 635/1000ml at 0.20ml/s with 25.2psi vacuum 5.4m deep (22.7psia)
@14:37:53.62 Cartridge 9 filtering 648/1000ml at 0.20ml/s with 25.9psi vacuum 5.5m deep (22.9psia)
@14:38:53.62 Cartridge 9 filtering 660/1000ml at 0.20ml/s with 26.4psi vacuum 6.0m deep (23.5psia)
@14:39:53.62 Cartridge 9 filtering 672/1000ml at 0.20ml/s with 25.2psi vacuum 5.1m deep (22.2psia)
@14:40:53.62 Cartridge 9 filtering 684/1000ml at 0.20ml/s with 25.9psi vacuum 6.5m deep (24.4psia)
@14:41:53.62 Cartridge 9 filtering 696/1000ml at 0.20ml/s with 26.6psi vacuum 6.1m deep (23.7psia)
@14:41:58.40 <FILTERING> Flow averaged 0.26ml/s over 44:08
@14:41:58.44 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi
@14:41:59.02 Relieved excess vacuum in .6 seconds
@14:41:59.05 SamplePump.seek 1000ml,25:45
@14:42:01.19 Flow estimate reduced to 0.21ml/s
@14:42:01.23 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi
@14:42:01.81 Relieved excess vacuum in .6 seconds
@14:42:01.84 SamplePump.seek 1000ml,25:43
@14:42:53.62 <sampling> Cartridge 9 filtering 708/1000ml at 0.20ml/s with 26.2psi vacuum 5.0m deep (22.1psia)
@14:43:36.68 <FILTERING> Flow averaged 0.20ml/s over 1:35
@14:43:36.73 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi
@14:43:37.31 Relieved excess vacuum in .6 seconds
@14:43:37.34 SamplePump.seek 1000ml,24:07
@14:43:53.61 <sampling> Cartridge 9 filtering 720/1000ml at 0.20ml/s with 25.9psi vacuum 6.4m deep (24.1psia)
@14:44:21.24 <FILTERING> Flow averaged 0.20ml/s over 44 seconds
@14:44:21.28 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@14:44:21.88 Relieved excess vacuum in .6 seconds
@14:44:21.91 SamplePump.seek 1000ml,23:23
@14:44:42.77 Flow averaged 0.20ml/s over 21 seconds
@14:44:42.79 <closeIntake> Intake.close
@14:44:42.83 <closeExhaust> Exhaust.close
@14:44:43.08 <FILTERING> Toroid.in :hold
@14:44:43.20 Toroid.seek :bypass
@14:44:44.86 Toroid.in :default
@14:44:44.94 Toroid.seek :clear
@14:44:46.56 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@14:44:46.58 Pressure.stop
@14:44:46.77 Sampled  729.6ml
@14:44:48.81 <LRAUV> -> Cmd.startProcessing
@14:44:50.25 <PROCESSING> SPR.command \"read_sequences\"
@14:44:51.05 SPR.command \"stats_on\"
@14:44:51.60 SPR.command \"start 0 1\"
@14:44:51.90 <SPRlogger> SPR.start /var/log/esp/spr23apr2514h.spr.bz2
@14:44:54.38 <PROCESSING> Handoff.select :waste
@14:45:01.07 Syringe tare is 16% of full scale
@14:45:01.09 PinPort.in :locking
@14:45:01.17 PinPort.seek :locked
@14:45:01.65 <posArchive> Archive.in :fast
@14:45:01.71 <posLyS2> Lysis2.in :fast
@14:45:01.75 <PROCESSING> Lysis1.seek 1350uL
@14:45:01.88 <posArchive> Archive.seek :full
@14:45:01.96 <posLyS2> Lysis2.seek :full
@14:45:03.95 <PROCESSING> Plunge.seek :reagent
@14:45:04.88 Evacuating puck with filter bubble point of 50psi
@14:45:04.89 PinPort.seek :locked
@14:45:04.99 Handoff.select :waste
@14:45:12.09 Air syringe pumping up to 1 strokes to reach 8psi
@14:45:12.11 Air stroke #1 of 1
@14:45:12.20 Air.in :pressurize
@14:45:12.28 Air.seek :pressurized
@14:45:18.08 1.4psi after pressurize
@14:45:18.10 Air.in :compress
@14:45:18.18 Air.seek :evac
@14:45:21.18 0.6psi after compress
@14:45:31.76 -0.1psi after compress (stable)
@14:45:31.77 Air.in :default
@14:45:31.84 Air.hold
@14:45:32.04 at -0.1psi Opened to waste
@14:45:32.05 Handoff.select :blocked
@14:45:32.20 Archive syringe pumping up to 8 strokes to reach 50psi
@14:45:32.22 Archive stroke #1 of 8
@14:45:32.30 Archive.in :pressurize
@14:45:32.37 Archive.seek :pressurized
@14:45:44.87 0.1psi after pressurize
@14:45:50.44 0.1psi after pressurize (stable)
@14:45:50.46 Archive.in :compress
@14:45:50.54 Archive.seek :empty
@14:46:05.54 3.6psi after compress
@14:46:16.11 3.8psi after compress (stable)
@14:46:16.13 Archive.in :gulp
@14:46:16.22 Archive.seek :topVent
@14:46:22.45 3.9psi after gulp
@14:46:28.05 4.0psi after gulp (stable)
@14:46:28.06 Archive stroke #2 of 8
@14:46:28.17 Archive.in :pressurize
@14:46:28.24 Archive.seek :pressurized
@14:46:40.73 4.2psi after pressurize
@14:46:46.30 4.3psi after pressurize (stable)
@14:46:46.32 Archive.in :compress
@14:46:46.40 Archive.seek :empty
@14:47:01.02 3.9psi after compress
@14:47:36.12 18.8psi after compress (stable)
@14:47:36.16 Archive.in :gulp
@14:47:36.25 Archive.seek :topVent
@14:47:42.49 20.0psi after gulp
@14:47:48.06 20.5psi after gulp (stable)
@14:47:48.07 Archive stroke #3 of 8
@14:47:48.18 Archive.in :pressurize
@14:47:48.26 Archive.seek :pressurized
@14:48:00.77 21.2psi after pressurize
@14:48:06.38 21.5psi after pressurize (stable)
@14:48:06.41 Archive.in :compress
@14:48:06.50 Archive.seek :empty
@14:48:21.12 25.7psi after compress
@14:48:54.97 36.7psi after compress (stable)
@14:48:55.00 Archive.in :gulp
@14:48:55.08 Archive.seek :topVent
@14:49:01.32 37.8psi after gulp
@14:49:06.91 38.2psi after gulp (stable)
@14:49:06.93 Archive stroke #4 of 8
@14:49:07.03 Archive.in :pressurize
@14:49:07.11 Archive.seek :pressurized
@14:49:19.59 38.6psi after pressurize
@14:49:25.16 38.8psi after pressurize (stable)
@14:49:25.18 Archive.in :compress
@14:49:25.27 Archive.seek :empty
@14:49:39.89 42.8psi after compress
@14:49:51.43 Reached 50.1 of 50.0psi after pumping 6920uL (4.00 strokes)
@14:49:51.44 Archive.in :default
@14:49:51.53 Archive.hold
@14:49:51.60 Handoff.select :waste
@14:50:06.79 delay 1:00
@14:51:06.81 Handoff.select :blocked
@14:51:29.49 Handoff.select :waste
@14:51:29.62 0.2psi at evacEnd
@14:51:29.63 Handoff.select :blocked
@14:51:29.79 Archive.in :fast
@14:51:29.85 Archive.seek :topVent
@14:51:43.53 Stable @0.3psi after Handoff blocked
@14:51:43.55 Archive syringe pumping up to 8 strokes to reach 30psi
@14:51:43.57 Archive stroke #1 of 8
@14:51:43.67 Archive.in :pressurize
@14:51:43.74 Archive.seek :pressurized
@14:51:56.27 0.4psi after pressurize
@14:51:56.30 Archive.in :compress
@14:51:56.38 Archive.seek :empty
@14:52:11.05 2.9psi after compress
@14:52:33.16 7.8psi after compress (stable)
@14:52:33.18 Archive.in :gulp
@14:52:33.27 Archive.seek :topVent
@14:52:39.52 8.6psi after gulp
@14:52:45.09 8.9psi after gulp (stable)
@14:52:45.11 Archive stroke #2 of 8
@14:52:45.21 Archive.in :pressurize
@14:52:45.28 Archive.seek :pressurized
@14:52:57.78 9.6psi after pressurize
@14:52:57.79 Archive.in :compress
@14:52:57.87 Archive.seek :empty
@14:53:12.77 15.5psi after compress
@14:53:37.47 22.4psi after compress (stable)
@14:53:37.49 Archive.in :gulp
@14:53:37.57 Archive.seek :topVent
@14:53:43.81 23.2psi after gulp
@14:53:49.39 23.5psi after gulp (stable)
@14:53:49.40 Archive stroke #3 of 8
@14:53:49.50 Archive.in :pressurize
@14:53:49.57 Archive.seek :pressurized
@14:54:02.09 24.2psi after pressurize
@14:54:02.11 Archive.in :compress
@14:54:02.19 Archive.seek :empty
@14:54:16.82 28.8psi after compress
@14:54:18.75 Reached 30.4 of 30.3psi after pumping 5190uL (3.00 strokes)
@14:54:18.77 Archive.in :default
@14:54:18.85 Archive.hold
@14:54:18.92 Handoff.select :waste
@14:54:33.11 delay 1:00
@14:55:33.13 Handoff.select :blocked
@14:55:55.81 Handoff.select :waste
@14:55:55.94 0.2psi at evacEnd
@14:55:55.96 Air.seek :fullVent
@14:55:57.74 Archive.in :fast
@14:55:57.81 Archive.seek :topVent
@14:56:04.93 Seawater evacuated, Pressure above/below filter released.
@14:56:05.06 Lysis1.in :slow
@14:56:05.13 Lysis1.seek 1050uL
@14:56:35.17 delay 5 seconds
@14:56:40.18 Handoff.select :blocked
@14:56:40.37 Lysis1.seek 450uL
@14:57:40.36 delay 3 seconds
@14:57:43.44 Air.in mix with 1.40048 parts Lysis1
@14:57:43.87 <Airmixer> Air.seek :evac
@14:57:43.91 <PROCESSING> Lysis1.seek 300uL
@14:57:59.02 Lysis1.seek :empty
@14:58:29.13 Lysis buffer delivered @13.6psi
@14:58:29.15 Polling Can every 2:00
@14:58:29.69 PuckHeater.configure :default #ambient 11.7C
@14:58:29.75 PuckHeater.seek 80.2C
@14:59:34.82 PuckHeater.hold 2:00,80.2C
@15:01:34.50 Polling Can every 2:00
@15:01:35.05 PuckHeater.configure :default #ambient 11.8C
@15:01:35.14 PuckHeater.hold 30 seconds,79.2C
@15:02:05.23 Polling Can every 2:00
@15:02:05.77 PuckHeater.configure :default #ambient 11.8C
@15:02:05.83 PuckHeater.hold 30 seconds,78.6C
@15:02:35.93 Polling Can every 2:00
@15:02:36.47 PuckHeater.configure :default #ambient 11.7C
@15:02:36.56 PuckHeater.hold 10:00,78.1C
@15:12:35.02 Lysis complete. Syringe pressure @21.1psi
@15:12:35.04 Archive syringe pumping up to 5 strokes to reach 40psi
@15:12:35.05 Archive stroke #1 of 5
@15:12:35.15 Archive.in :pressurize
@15:12:35.24 Archive.seek :pressurized
@15:12:47.76 20.5psi after pressurize
@15:12:53.34 20.3psi after pressurize (stable)
@15:12:53.36 Archive.in :compress
@15:12:53.44 Archive.seek :empty
@15:13:01.20 Reached 40.3 of 40.0psi after pumping 1582uL (0.91 strokes)
@15:13:01.21 Archive.in :default
@15:13:01.28 Archive.hold
@15:13:01.36 delay 2 seconds
@15:13:03.38 Archive.in :fast
@15:13:03.44 Archive.seek :full
@15:13:10.10 Lysis1.seek 138uL
@15:13:23.98 delay 1 second
@15:13:25.00 <fillArchive> Archive.seek :full
@15:13:25.04 <PROCESSING> Lysis1.seek :empty
@15:13:38.98 Handoff.select :blocked
@15:13:39.08 Initial air pressure @41.0psi
@15:13:39.15 Lysis1.in :extract
@15:13:39.24 Lysis1.seek 600uL,nil
@15:13:42.63 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@15:13:42.64 Archive stroke #1 of 6
@15:13:42.73 Archive.in :pressurize
@15:13:42.82 Archive.seek :pressurized
@15:13:55.33 14.5psi after pressurize
@15:14:03.82 Archive.in :default
@15:14:03.88 Archive.hold
@15:14:04.01 <PROCESSING> Initial 600uL lysate @14.3psi
@15:14:04.02 Handoff.select :blocked
@15:14:04.14 Initial air pressure @14.7psi
@15:14:04.22 Lysis1.seek 825uL,nil
@15:14:04.33 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@15:14:04.35 Archive stroke #1 of 6
@15:14:04.50 Archive.in :compress
@15:14:04.59 Archive.seek :empty
@15:14:19.23 13.7psi after compress
@15:14:29.81 13.7psi after compress (stable)
@15:14:34.99 Archive.in :gulp
@15:14:35.07 Archive.seek :topVent
@15:14:41.40 14.0psi after gulp
@15:14:46.99 14.1psi after gulp (stable)
@15:14:47.00 Archive stroke #2 of 6
@15:14:47.10 Archive.in :pressurize
@15:14:47.18 Archive.seek :pressurized
@15:15:00.12 13.9psi after pressurize
@15:15:05.74 14.0psi after pressurize (stable)
@15:15:05.76 Archive.in :compress
@15:15:05.85 Archive.seek :empty
@15:15:19.43 Archive.in :default
@15:15:19.49 Archive.hold
@15:15:19.70 <PROCESSING> Recovered 825uL lysate @15.8psi
@15:15:19.73 Air.in :halfSpeed
@15:15:19.82 Air.seek :fullVent
@15:15:22.04 Puck Top Vented, bottom @2.2psi
@15:15:22.05 Handoff.select :waste
@15:15:22.17 delay 5 seconds
@15:15:27.24 Handoff open to waste, bottom @0.5psi
@15:15:27.25 Air.in :default
@15:15:27.33 Air.seek :evac
@15:15:28.77 Lysis2.in :DAmix
@15:15:28.89 Lysis1.in :DAmix
@15:15:29.33 <Lysis2mixer> Lysis2.seek 1480uL
@15:15:29.38 <PROCESSING> Lysis1.seek 575uL
@15:16:04.49 delay 5 seconds
@15:16:09.50 Handoff.select SPR
@15:16:09.63 SPR.command \"start 2 14\"
@15:16:10.43 SPR stabilized
@15:16:10.56 delay 10 seconds
@15:16:20.58 SPR.command \"start 1 0\"
@15:16:23.79 Waiting up to 4:00 for SPR to enter state 1
@15:18:28.05 SPR.command \"hold_on\"
@15:18:29.95 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@15:18:30.40 <Lysis2mixer> Lysis2.seek 1180uL
@15:18:30.44 <PROCESSING> Lysis1.seek 275uL
@15:19:12.50 delay 15 seconds
@15:19:27.53 SPR.command \"hold_off\"
@15:19:30.12 Waiting up to 38:00 for SPR to enter state 7
@15:37:28.75 <SPRlogger> SPRsummary:-25.5RIU,150RIU,146RIU,230RIU,none,none,none after 3335s
@15:44:05.98 <PROCESSING> SPR analysis completed
@15:44:12.82 <SPRlogger> Ended log spr23apr2514h.spr.bz2
@15:44:12.83 <PROCESSING> SPR.command \"stats_off\"
@15:44:14.30 <LRAUV> -> Cmd.stop
@15:44:14.40 <closeHandoff> Handoff.select :close
@15:44:14.53 <LRAUV> Gate.power :heat,:OFF
@15:44:14.55 Gate.power :cartridge,:OFF
@15:44:14.64 PinPort.in :unlocking
@15:44:14.71 PinPort.seek :clear
@15:44:15.18 Carousel.in :default
@15:44:15.29 Carousel.home.seek 9
@15:44:15.59 Plunge.seek :locked
@15:44:16.46 <Archive> Archive.in :unlocking
@15:44:16.59 Archive.seek :clear
@15:44:16.64 <Air> Air.in :unlocking
@15:44:16.81 Air.seek :clear
@15:44:16.85 <Lysis1> Lysis1.in :unlocking
@15:44:17.02 Lysis1.seek :clear
@15:44:17.06 <Lysis2> Lysis2.in :unlocking
@15:44:17.17 Lysis2.seek :clear
@15:44:21.20 <LRAUV> Gate.power :motor,:OFF
@15:44:21.21 Gate.power :core,:OFF
@15:44:21.24 Gate.power :analytic1,:OFF
@15:44:21.26 Gate.power :analytic2,:OFF
@15:44:21.28 Gate.power :analytic3,:OFF
@15:44:21.30 Gate.power :main,:OFF
@15:44:21.32 Safely stopped and ready to power off
##-- file closed 2023-04-25T23:01:04.713Z

##-- file opened 2023-04-25T23:01:04.715Z
@16:01:05.04 -> showStatus
@16:01:07.11 -> Cmd.loadCartridge -10
@16:01:07.17 Gate.power :main,:ON,limit to 5000mA
@16:01:07.18 Gate.power :analytic1,:ON,limit to 5000mA
@16:01:07.21 Gate.power :analytic2,:ON,limit to 5000mA
@16:01:07.24 Gate.power :analytic3,:ON,limit to 5000mA
@16:01:07.42 Powering core bus
@16:01:09.64 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@16:01:10.11 <LRAUV> Archive.configure :default
@16:01:10.20 Air.configure :default
@16:01:10.27 Lysis1.configure :default
@16:01:10.34 Lysis2.configure :default
@16:01:10.43 Plunge.configure :default
@16:01:10.52 SamplePump.configure :default
@16:01:10.61 Toroid.configure :default
@16:01:10.70 Carousel.configure :default
@16:01:10.79 Pressure.configure :default
@16:01:10.88 ExtraPump.configure :default
@16:01:10.96 PinPort.configure :default
@16:01:11.03 All elf controllers running firmware version 4.83
@16:01:11.05 Gate.power :motor,:ON,limit to 5000mA
@16:01:11.19 <Archive_home> Archive.home.jog 0
@16:01:11.71 <Carousel_home> Carousel.home.jog 0
@16:01:11.80 <Toroid_home> Toroid.home.jog 0
@16:01:11.83 <Lysis2_home> Lysis2.home.jog 0
@16:01:11.86 <Lysis1_home> Lysis1.home.jog 0
@16:01:11.90 <PinPort_home> PinPort.home.jog 0
@16:01:11.95 <Plunge_home> Plunge.home.jog 0
@16:01:11.99 <Air_home> Air.home.jog 0
@16:01:12.62 <Archive_home> Archive.in :homing
@16:01:12.84 <Plunge_home> Plunge.in :homing
@16:01:12.87 <Archive_home> Archive.jog 1392
@16:01:12.90 <PinPort_home> PinPort.in :homing
@16:01:12.93 <Lysis1_home> Lysis1.in :homing
@16:01:12.98 <Air_home> Air.in :homing
@16:01:13.02 <Toroid_home> Toroid.in :homing
@16:01:13.06 <Lysis2_home> Lysis2.in :homing
@16:01:13.30 <Plunge_home> Plunge.jog 1559
@16:01:13.36 <PinPort_home> PinPort.jog -106
@16:01:13.38 <Lysis1_home> Lysis1.jog 156
@16:01:13.41 <Air_home> Air.jog 1390
@16:01:13.44 <Toroid_home> Toroid.jog 633
@16:01:13.47 <Lysis2_home> Lysis2.jog 1404
@16:01:14.12 <PinPort_home> PinPort.setRawPosition! 0
@16:01:14.14 <Lysis1_home> Lysis1.setRawPosition! 50
@16:01:14.24 Lysis1.seek :home
@16:01:14.29 <PinPort_home> PinPort.seek :home
@16:01:14.33 <Archive_home> Archive.setRawPosition! 50
@16:01:14.48 Archive.seek :home
@16:01:14.51 <PinPort_home> PinPort.in :default
@16:01:14.65 <Lysis1_home> Lysis1.in :default
@16:01:14.74 <Plunge_home> Plunge.setRawPosition! 50
@16:01:14.79 Plunge.seek :home
@16:01:14.88 <Archive_home> Archive.in :default
@16:01:15.07 <Plunge_home> Plunge.in :default
@16:01:15.17 <Air_home> Air.setRawPosition! 50
@16:01:15.22 Air.seek :home
@16:01:15.31 <Lysis2_home> Lysis2.setRawPosition! 50
@16:01:15.35 Lysis2.seek :home
@16:01:15.51 <Air_home> Air.in :default
@16:01:15.64 <Lysis2_home> Lysis2.in :default
@16:01:16.11 <Toroid_home> Toroid.setRawPosition! 0
@16:01:16.16 Toroid.seek :home
@16:01:16.25 Toroid.in :default
@16:01:16.32 <LRAUV> Toroid.seek :clear
@16:01:17.94 Carousel.in :default
@16:01:18.06 Carousel.home.seek 9
@16:01:18.32 Selecting Cartridge 7
@16:01:18.58 Carousel.seek 7
@16:01:19.27 Lysis1.in :fast
@16:01:19.34 Lysis1.seek :locked
@16:01:21.95 <Plunge> Plunge.seek :locked
@16:01:22.09 <Air> Air.in :fast
@16:01:22.15 Air.seek :locked
@16:01:23.73 <LRAUV> Carousel.reconfigure :locked
@16:01:23.87 Powering cartridge bus
@16:01:26.49 Gate.setIR 1750,5000,0,6
@16:01:26.61 Gate.setIR 0,5000,0,6
@16:01:26.68 PuckHeater.configure :default
@16:01:26.74 Gate.power :heat,:ON,limit to 5000mA
@16:01:27.41 Mounted lysis Cartridge board#Q05036
@16:01:27.45 Using default cartridge heat loss curve for HeatingAccelInCarousel
@16:01:44.63 -> Cmd.startFiltering
@16:01:44.87 <FILTERING> Preparing to filter cartridge 7
@16:01:45.16 Priming sample loop w/100ml, bypass w/10ml
@16:01:45.17 SamplePump.setPosition! 0ml
@16:01:45.23 Bypass.open
@16:01:46.70 Intake.open
@16:01:47.39 Sea 7.0m deep (25.0psia)
@16:01:47.41 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@16:01:50.35 Vacuum tare is 60% of full scale
@16:01:50.50 Pressure.in :default
@16:01:50.57 Pressure.hold
@16:01:50.75 SamplePump.in :prime
@16:01:50.82 SamplePump.seek 10ml,59 seconds
@16:01:59.59 Pressure.stop
@16:01:59.64 SamplePump.setPosition! 0ml
@16:01:59.70 Exhaust.open
@16:02:01.13 <closeBypass> Bypass.close
@16:02:02.66 <FILTERING> Pressure.in :default
@16:02:02.72 Pressure.hold
@16:02:02.85 Sea 7.7m deep (26.0psia)
@16:02:02.99 SamplePump.in :prime
@16:02:03.07 SamplePump.seek 100ml,5:16
@16:03:40.89 Pressure.stop
@16:03:40.97 Bypass.open
@16:03:41.70 Intake.close
@16:03:42.42 Toroid.in :hold
@16:03:42.49 Toroid.seek :crack
@16:03:44.02 Toroid.seek :cartridge
@16:03:45.12 Toroid.in :default
@16:03:45.19 Toroid.seek :clear
@16:03:47.15 Pressurizing cartridge to 26psi above 25.4psia
@16:03:47.16 SamplePump.setPosition! 0ml
@16:03:47.22 Intake.open
@16:03:47.87 Bypass.close
@16:03:48.55 Exhaust.close
@16:03:49.37 Pressure.in :default
@16:03:49.43 Pressure.hold
@16:03:49.62 SamplePump.in :charge
@16:03:49.69 SamplePump.seek 2.3ml,42 seconds
@16:04:00.60 Pressure.stop
@16:04:00.66 Intake.close
@16:04:01.41 Pressurized cartridge with 0.563ml to 26.1psi
@16:04:01.42 Isolated @48.8psia (23.4psi)
@16:04:01.44 Verifying cartridge holds at least 0.65..0.85 of 48.8psia
@16:04:01.47 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@16:04:06.12 Sampling 1000.0ml in 1:25:20
@16:04:06.13 SamplePump.setPosition! 0ml
@16:04:06.20 Exhaust.open
@16:04:06.97 Intake.open
@16:04:07.78 Pressure.in :default
@16:04:07.85 Pressure.hold
@16:04:08.36 Sea 7.1m deep (25.1psia)
@16:04:08.37 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s
@16:04:08.53 SamplePump.in :sample
@16:04:08.60 SamplePump.seek 1000ml,1:23:51
@16:04:10.81 <sampling> Cartridge 7 filtering 1/1000ml at 0.41ml/s with 24.9psi vacuum 5.2m deep (22.4psia)
@16:05:10.82 Cartridge 7 filtering 25/1000ml at 0.39ml/s with 26.4psi vacuum 6.2m deep (23.9psia)
@16:06:10.82 Cartridge 7 filtering 48/1000ml at 0.37ml/s with 24.3psi vacuum 6.7m deep (24.6psia)
@16:07:10.82 Cartridge 7 filtering 71/1000ml at 0.39ml/s with 24.7psi vacuum 6.7m deep (24.7psia)
@16:08:10.82 Cartridge 7 filtering 93/1000ml at 0.38ml/s with 23.5psi vacuum 5.2m deep (22.3psia)
@16:09:10.82 Cartridge 7 filtering 116/1000ml at 0.37ml/s with 25.9psi vacuum 5.0m deep (22.0psia)
@16:10:10.82 Cartridge 7 filtering 137/1000ml at 0.36ml/s with 22.5psi vacuum 6.0m deep (23.6psia)
@16:11:10.82 Cartridge 7 filtering 159/1000ml at 0.34ml/s with 26.2psi vacuum 4.1m deep (20.8psia)
@16:12:10.82 Cartridge 7 filtering 180/1000ml at 0.36ml/s with 21.8psi vacuum 7.5m deep (25.8psia)
@16:13:10.82 Cartridge 7 filtering 201/1000ml at 0.33ml/s with 24.1psi vacuum 4.7m deep (21.6psia)
@16:14:10.82 Cartridge 7 filtering 222/1000ml at 0.35ml/s with 24.1psi vacuum 6.7m deep (24.7psia)
@16:15:10.82 Cartridge 7 filtering 242/1000ml at 0.32ml/s with 23.7psi vacuum 5.1m deep (22.3psia)
@16:16:10.82 Cartridge 7 filtering 262/1000ml at 0.34ml/s with 25.0psi vacuum 6.2m deep (23.8psia)
@16:17:10.81 Cartridge 7 filtering 281/1000ml at 0.33ml/s with 23.3psi vacuum 5.2m deep (22.5psia)
@16:18:10.82 Cartridge 7 filtering 301/1000ml at 0.30ml/s with 22.9psi vacuum 5.6m deep (22.9psia)
@16:19:10.83 Cartridge 7 filtering 320/1000ml at 0.31ml/s with 26.7psi vacuum 5.1m deep (22.2psia)
@16:20:10.81 Cartridge 7 filtering 338/1000ml at 0.32ml/s with 24.2psi vacuum 4.5m deep (21.4psia)
@16:21:10.83 Cartridge 7 filtering 357/1000ml at 0.32ml/s with 21.9psi vacuum 6.7m deep (24.7psia)
@16:22:10.82 Cartridge 7 filtering 375/1000ml at 0.31ml/s with 23.3psi vacuum 5.4m deep (22.7psia)
@16:23:10.83 Cartridge 7 filtering 393/1000ml at 0.31ml/s with 24.5psi vacuum 5.7m deep (23.1psia)
@16:24:10.82 Cartridge 7 filtering 410/1000ml at 0.28ml/s with 24.3psi vacuum 5.7m deep (23.1psia)
@16:25:10.87 Cartridge 7 filtering 428/1000ml at 0.30ml/s with 25.9psi vacuum 5.3m deep (22.5psia)
@16:26:10.82 Cartridge 7 filtering 445/1000ml at 0.28ml/s with 24.1psi vacuum 4.7m deep (21.7psia)
@16:27:10.82 Cartridge 7 filtering 462/1000ml at 0.27ml/s with 24.0psi vacuum 6.2m deep (23.8psia)
@16:28:10.82 Cartridge 7 filtering 478/1000ml at 0.26ml/s with 23.5psi vacuum 6.0m deep (23.5psia)
@16:29:10.82 Cartridge 7 filtering 494/1000ml at 0.27ml/s with 22.5psi vacuum 5.7m deep (23.2psia)
@16:30:10.87 Cartridge 7 filtering 510/1000ml at 0.26ml/s with 25.6psi vacuum 6.3m deep (24.0psia)
@16:31:10.82 Cartridge 7 filtering 526/1000ml at 0.26ml/s with 22.8psi vacuum 4.3m deep (21.0psia)
@16:32:10.82 Cartridge 7 filtering 542/1000ml at 0.27ml/s with 24.7psi vacuum 6.8m deep (24.7psia)
@16:33:10.82 Cartridge 7 filtering 557/1000ml at 0.27ml/s with 24.5psi vacuum 5.5m deep (22.8psia)
@16:34:10.82 Cartridge 7 filtering 572/1000ml at 0.25ml/s with 23.0psi vacuum 6.6m deep (24.4psia)
@16:35:10.87 Cartridge 7 filtering 587/1000ml at 0.24ml/s with 22.8psi vacuum 6.2m deep (23.8psia)
@16:36:10.82 Cartridge 7 filtering 601/1000ml at 0.23ml/s with 23.7psi vacuum 5.1m deep (22.2psia)
@16:37:11.13 Cartridge 7 filtering 616/1000ml at 0.26ml/s with 25.4psi vacuum 6.6m deep (24.5psia)
@16:38:10.82 Cartridge 7 filtering 630/1000ml at 0.25ml/s with 24.3psi vacuum 5.8m deep (23.3psia)
@16:39:10.81 Cartridge 7 filtering 644/1000ml at 0.23ml/s with 23.4psi vacuum 7.7m deep (26.0psia)
@16:40:10.82 Cartridge 7 filtering 658/1000ml at 0.22ml/s with 23.5psi vacuum 5.9m deep (23.4psia)
@16:41:10.82 Cartridge 7 filtering 672/1000ml at 0.22ml/s with 23.4psi vacuum 6.7m deep (24.6psia)
@16:42:10.83 Cartridge 7 filtering 685/1000ml at 0.21ml/s with 23.5psi vacuum 6.0m deep (23.5psia)
@16:43:10.83 Cartridge 7 filtering 698/1000ml at 0.21ml/s with 22.8psi vacuum 5.0m deep (22.1psia)
@16:44:10.83 Cartridge 7 filtering 712/1000ml at 0.21ml/s with 24.6psi vacuum 4.2m deep (21.0psia)
@16:45:10.82 Cartridge 7 filtering 725/1000ml at 0.21ml/s with 24.1psi vacuum 5.3m deep (22.5psia)
@16:46:10.83 Cartridge 7 filtering 738/1000ml at 0.20ml/s with 24.9psi vacuum 5.8m deep (23.2psia)
@16:47:10.83 Cartridge 7 filtering 750/1000ml at 0.21ml/s with 25.0psi vacuum 5.5m deep (22.8psia)
@16:48:10.82 Cartridge 7 filtering 763/1000ml at 0.20ml/s with 24.4psi vacuum 5.3m deep (22.6psia)
@16:49:10.83 Cartridge 7 filtering 775/1000ml at 0.22ml/s with 22.8psi vacuum 5.9m deep (23.4psia)
@16:50:10.82 Cartridge 7 filtering 788/1000ml at 0.22ml/s with 24.4psi vacuum 5.0m deep (22.1psia)
@16:51:10.82 Cartridge 7 filtering 800/1000ml at 0.21ml/s with 24.2psi vacuum 6.0m deep (23.5psia)
@16:52:10.82 Cartridge 7 filtering 812/1000ml at 0.20ml/s with 24.6psi vacuum 4.6m deep (21.6psia)
@16:53:10.82 Cartridge 7 filtering 824/1000ml at 0.20ml/s with 25.6psi vacuum 6.5m deep (24.4psia)
@16:54:10.83 Cartridge 7 filtering 837/1000ml at 0.20ml/s with 26.3psi vacuum 5.8m deep (23.3psia)
@16:55:10.82 Cartridge 7 filtering 849/1000ml at 0.20ml/s with 23.8psi vacuum 4.6m deep (21.5psia)
@16:56:10.82 Cartridge 7 filtering 861/1000ml at 0.20ml/s with 26.3psi vacuum 5.8m deep (23.3psia)
@16:57:10.83 Cartridge 7 filtering 873/1000ml at 0.20ml/s with 25.7psi vacuum 4.7m deep (21.7psia)
@16:58:02.04 <FILTERING> Flow averaged 0.27ml/s over 53:54
@16:58:02.08 Waiting up to 8 seconds for vacuum to reduce from 26.0 to 18.0psi
@16:58:02.67 Relieved excess vacuum in .6 seconds
@16:58:02.69 SamplePump.seek 1000ml,10:14
@16:58:10.81 <sampling> Cartridge 7 filtering 885/1000ml at 0.20ml/s with 25.9psi vacuum 6.4m deep (24.2psia)
@16:59:10.83 Cartridge 7 filtering 897/1000ml at 0.20ml/s with 24.4psi vacuum 4.9m deep (21.9psia)
@16:59:39.38 <FILTERING> Flow averaged 0.20ml/s over 1:37
@16:59:39.41 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi
@16:59:40.00 Relieved excess vacuum in .6 seconds
@16:59:40.03 SamplePump.seek 1000ml,8:37
@16:59:43.43 Flow estimate reduced to 0.16ml/s
@16:59:43.45 <closeIntake> Intake.close
@16:59:43.54 <closeExhaust> Exhaust.close
@16:59:43.73 <FILTERING> Toroid.in :hold
@16:59:43.80 Toroid.seek :bypass
@16:59:45.45 Toroid.in :default
@16:59:45.52 Toroid.seek :clear
@16:59:47.14 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@16:59:47.16 Pressure.stop
@16:59:47.35 Sampled  903.4ml
@16:59:49.39 <LRAUV> -> Cmd.startProcessing
@16:59:51.28 <PROCESSING> SPR.command \"read_sequences\"
@16:59:51.79 SPR.command \"stats_on\"
@16:59:51.93 SPR.command \"start 0 1\"
@16:59:52.65 <SPRlogger> SPR.start /var/log/esp/spr23apr2516h.spr.bz2
@16:59:54.64 <PROCESSING> Handoff.select :waste
@17:00:01.33 Syringe tare is 16% of full scale
@17:00:01.35 PinPort.in :locking
@17:00:01.43 PinPort.seek :locked
@17:00:01.91 <posArchive> Archive.in :fast
@17:00:01.97 <posLyS2> Lysis2.in :fast
@17:00:02.04 <PROCESSING> Lysis1.seek 1350uL
@17:00:02.13 <posArchive> Archive.seek :full
@17:00:02.24 <posLyS2> Lysis2.seek :full
@17:00:04.20 <PROCESSING> Plunge.seek :reagent
@17:00:05.08 Evacuating puck with filter bubble point of 50psi
@17:00:05.10 PinPort.seek :locked
@17:00:05.18 Handoff.select :waste
@17:00:11.77 Air syringe pumping up to 1 strokes to reach 8psi
@17:00:11.78 Air stroke #1 of 1
@17:00:11.88 Air.in :pressurize
@17:00:11.95 Air.seek :pressurized
@17:00:17.68 1.2psi after pressurize
@17:00:17.69 Air.in :compress
@17:00:17.80 Air.seek :evac
@17:00:20.84 1.0psi after compress
@17:00:31.41 0.1psi after compress (stable)
@17:00:31.42 Air.in :default
@17:00:31.54 Air.hold
@17:00:31.72 at 0.0psi Opened to waste
@17:00:31.73 Handoff.select :blocked
@17:00:31.87 Archive syringe pumping up to 8 strokes to reach 50psi
@17:00:31.88 Archive stroke #1 of 8
@17:00:31.98 Archive.in :pressurize
@17:00:32.07 Archive.seek :pressurized
@17:00:44.58 1.7psi after pressurize
@17:00:50.16 2.2psi after pressurize (stable)
@17:00:50.17 Archive.in :compress
@17:00:50.26 Archive.seek :empty
@17:01:04.89 14.6psi after compress
@17:01:37.96 24.4psi after compress (stable)
@17:01:37.98 Archive.in :gulp
@17:01:38.07 Archive.seek :topVent
@17:01:44.32 25.3psi after gulp
@17:01:49.89 25.8psi after gulp (stable)
@17:01:49.90 Archive stroke #2 of 8
@17:01:50.00 Archive.in :pressurize
@17:01:50.07 Archive.seek :pressurized
@17:02:02.56 26.2psi after pressurize
@17:02:08.43 26.4psi after pressurize (stable)
@17:02:08.47 Archive.in :compress
@17:02:08.57 Archive.seek :empty
@17:02:23.19 32.1psi after compress
@17:02:58.28 44.4psi after compress (stable)
@17:02:58.29 Archive.in :gulp
@17:02:58.38 Archive.seek :topVent
@17:03:04.64 45.1psi after gulp
@17:03:10.21 45.2psi after gulp (stable)
@17:03:10.22 Archive stroke #3 of 8
@17:03:10.32 Archive.in :pressurize
@17:03:10.39 Archive.seek :pressurized
@17:03:22.89 45.0psi after pressurize
@17:03:28.46 45.1psi after pressurize (stable)
@17:03:28.48 Archive.in :compress
@17:03:28.56 Archive.seek :empty
@17:03:42.58 Reached 50.1 of 50.0psi after pumping 5177uL (2.99 strokes)
@17:03:42.59 Archive.in :default
@17:03:42.67 Archive.hold
@17:03:42.74 Handoff.select :waste
@17:03:55.94 delay 1:00
@17:04:55.95 Handoff.select :blocked
@17:05:36.19 Pressure too High, open to waste again (8.0psi)
@17:05:36.21 Handoff.select :waste
@17:05:36.35 delay 1:00
@17:06:36.37 Handoff.select :blocked
@17:06:59.08 Handoff.select :waste
@17:06:59.20 2.5psi at evacEnd
@17:06:59.21 Handoff.select :blocked
@17:06:59.35 Archive.in :fast
@17:06:59.42 Archive.seek :topVent
@17:07:13.07 Stable @1.8psi after Handoff blocked
@17:07:13.10 Archive syringe pumping up to 8 strokes to reach 32psi
@17:07:13.12 Archive stroke #1 of 8
@17:07:13.22 Archive.in :pressurize
@17:07:13.29 Archive.seek :pressurized
@17:07:25.79 3.2psi after pressurize
@17:07:25.80 Archive.in :compress
@17:07:25.89 Archive.seek :empty
@17:07:40.54 6.1psi after compress
@17:08:33.62 17.5psi after compress (stable)
@17:08:33.63 Archive.in :gulp
@17:08:33.72 Archive.seek :topVent
@17:08:39.97 18.6psi after gulp
@17:08:45.55 19.2psi after gulp (stable)
@17:08:45.56 Archive stroke #2 of 8
@17:08:45.66 Archive.in :pressurize
@17:08:45.76 Archive.seek :pressurized
@17:08:58.26 20.4psi after pressurize
@17:08:58.28 Archive.in :compress
@17:08:58.35 Archive.seek :empty
@17:09:12.99 23.1psi after compress
@17:09:46.05 Reached 31.8 of 31.8psi after pumping 3460uL (2.00 strokes)
@17:09:46.07 Archive.in :default
@17:09:46.15 Archive.hold
@17:09:46.25 Handoff.select :waste
@17:09:55.45 delay 1:00
@17:10:55.47 Handoff.select :blocked
@17:11:32.71 Pressure too High, open to waste again (8.0psi)
@17:11:32.72 Handoff.select :waste
@17:11:32.87 delay 1:00
@17:12:32.88 Handoff.select :blocked
@17:12:55.56 Handoff.select :waste
@17:12:55.68 2.5psi at evacEnd
@17:12:55.70 Air.seek :fullVent
@17:12:57.14 Archive.in :fast
@17:12:57.21 Archive.seek :topVent
@17:13:04.31 Seawater evacuated, Pressure above/below filter released.
@17:13:04.40 Lysis1.in :slow
@17:13:04.47 Lysis1.seek 1050uL
@17:13:34.53 delay 5 seconds
@17:13:39.54 Handoff.select :blocked
@17:13:39.73 Lysis1.seek 450uL
@17:14:39.76 delay 3 seconds
@17:14:42.84 Air.in mix with 1.40048 parts Lysis1
@17:14:43.32 <Airmixer> Air.seek :evac
@17:14:43.37 <PROCESSING> Lysis1.seek 300uL
@17:14:58.50 Lysis1.seek :empty
@17:15:28.65 Lysis buffer delivered @17.0psi
@17:15:28.68 Polling Can every 2:00
@17:15:29.23 PuckHeater.configure :default #ambient 12.3C
@17:15:29.28 PuckHeater.seek 80.2C
@17:16:40.26 PuckHeater.hold 2:00,80.2C
@17:18:40.12 Polling Can every 2:00
@17:18:40.67 PuckHeater.configure :default #ambient 12.3C
@17:18:40.75 PuckHeater.hold 30 seconds,79.1C
@17:19:10.88 Polling Can every 2:00
@17:19:11.44 PuckHeater.hold 30 seconds,78.6C
@17:19:41.58 Polling Can every 2:00
@17:19:42.13 PuckHeater.configure :default #ambient 12.3C
@17:19:42.22 PuckHeater.hold 10:00,78.1C
@17:29:41.41 Lysis complete. Syringe pressure @18.0psi
@17:29:41.43 Archive syringe pumping up to 5 strokes to reach 40psi
@17:29:41.45 Archive stroke #1 of 5
@17:29:41.54 Archive.in :pressurize
@17:29:41.62 Archive.seek :pressurized
@17:29:54.15 17.8psi after pressurize
@17:29:59.75 17.7psi after pressurize (stable)
@17:29:59.79 Archive.in :compress
@17:29:59.87 Archive.seek :empty
@17:30:08.39 Reached 40.5 of 40.0psi after pumping 1598uL (0.92 strokes)
@17:30:08.40 Archive.in :default
@17:30:08.49 Archive.hold
@17:30:08.56 delay 2 seconds
@17:30:10.59 Archive.in :fast
@17:30:10.65 Archive.seek :full
@17:30:17.35 Lysis1.seek 138uL
@17:30:31.24 delay 1 second
@17:30:32.26 <fillArchive> Archive.seek :full
@17:30:32.65 <PROCESSING> Lysis1.seek :empty
@17:30:46.58 Handoff.select :blocked
@17:30:46.68 Initial air pressure @43.1psi
@17:30:46.75 Lysis1.in :extract
@17:30:46.84 Lysis1.seek 600uL,nil
@17:30:50.29 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@17:30:50.30 Archive stroke #1 of 6
@17:30:50.39 Archive.in :pressurize
@17:30:50.46 Archive.seek :pressurized
@17:31:03.01 14.1psi after pressurize
@17:31:08.59 14.1psi after pressurize (stable)
@17:31:08.61 Archive.in :compress
@17:31:08.69 Archive.seek :empty
@17:31:13.98 Archive.in :default
@17:31:14.04 Archive.hold
@17:31:14.18 <PROCESSING> Initial 600uL lysate @14.7psi
@17:31:14.19 Handoff.select :blocked
@17:31:14.29 Initial air pressure @15.2psi
@17:31:14.37 Lysis1.seek 825uL,nil
@17:31:14.46 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@17:31:14.49 Archive stroke #1 of 6
@17:31:14.64 Archive.in :compress
@17:31:14.71 Archive.seek :empty
@17:31:15.31 Reached 16.6 of 17.0psi after pumping 1533uL (0.89 strokes)
@17:31:15.33 Archive.in :default
@17:31:15.42 Archive.hold
@17:31:15.69 Archive syringe pumping up to 6 strokes to reach 17psi
@17:31:15.70 Archive stroke #1 of 6
@17:31:15.83 Archive.in :compress
@17:31:15.91 Archive.seek :empty
@17:31:25.23 12.9psi after compress
@17:31:35.80 13.1psi after compress (stable)
@17:31:40.97 Archive.in :gulp
@17:31:41.06 Archive.seek :topVent
@17:31:47.65 13.4psi after gulp
@17:31:53.32 13.4psi after gulp (stable)
@17:31:53.34 Archive stroke #2 of 6
@17:31:53.43 Archive.in :pressurize
@17:31:53.51 Archive.seek :pressurized
@17:32:06.02 13.5psi after pressurize
@17:32:11.69 13.5psi after pressurize (stable)
@17:32:11.71 Archive.in :compress
@17:32:11.79 Archive.seek :empty
@17:32:26.39 Archive.in :default
@17:32:26.46 Archive.hold
@17:32:26.70 <PROCESSING> Recovered 825uL lysate @17.2psi
@17:32:26.72 Air.in :halfSpeed
@17:32:26.80 Air.seek :fullVent
@17:32:29.00 Puck Top Vented, bottom @30.0psi
@17:32:29.01 Handoff.select :waste
@17:32:29.14 delay 5 seconds
@17:32:34.21 Handoff open to waste, bottom @1.3psi
@17:32:34.23 Air.in :default
@17:32:34.31 Air.seek :evac
@17:32:35.72 Lysis1.in :DAmix
@17:32:35.87 Lysis2.in :DAmix
@17:32:36.32 <Lysis1mixer> Lysis1.seek 575uL
@17:32:36.39 <PROCESSING> Lysis2.seek 1480uL
@17:33:11.47 delay 5 seconds
@17:33:16.49 Handoff.select SPR
@17:33:16.63 SPR.command \"start 2 14\"
@17:33:17.91 SPR stabilized
@17:33:18.05 delay 10 seconds
@17:33:28.07 SPR.command \"start 1 0\"
@17:33:31.28 Waiting up to 4:00 for SPR to enter state 1
@17:35:35.54 SPR.command \"hold_on\"
@17:35:36.96 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@17:35:37.42 <Lysis1mixer> Lysis1.seek 275uL
@17:35:37.47 <PROCESSING> Lysis2.seek 1180uL
@17:36:19.54 delay 15 seconds
@17:36:34.55 SPR.command \"hold_off\"
@17:36:38.09 Waiting up to 38:00 for SPR to enter state 7
@17:54:38.14 <SPRlogger> SPRsummary:12.6RIU,153RIU,153RIU,213RIU,2.31ng/L,none,3.97ng/L after 3471s
@18:01:15.36 <PROCESSING> SPR analysis completed
@18:01:22.32 <SPRlogger> Ended log spr23apr2516h.spr.bz2
@18:01:22.33 <PROCESSING> SPR.command \"stats_off\"
@18:01:23.70 <LRAUV> -> Cmd.stop
@18:01:23.80 <closeHandoff> Handoff.select :close
@18:01:23.93 <LRAUV> Gate.power :heat,:OFF
@18:01:23.95 Gate.power :cartridge,:OFF
@18:01:24.04 PinPort.in :unlocking
@18:01:24.11 PinPort.seek :clear
@18:01:24.58 Carousel.in :default
@18:01:24.69 Carousel.home.seek 7
@18:01:25.00 Plunge.seek :locked
@18:01:25.87 <Archive> Archive.in :unlocking
@18:01:26.00 Archive.seek :clear
@18:01:26.04 <Air> Air.in :unlocking
@18:01:26.22 Air.seek :clear
@18:01:26.27 <Lysis1> Lysis1.in :unlocking
@18:01:26.79 Lysis1.seek :clear
@18:01:26.84 <Lysis2> Lysis2.in :unlocking
@18:01:26.94 Lysis2.seek :clear
@18:01:30.83 <LRAUV> Gate.power :motor,:OFF
@18:01:30.85 Gate.power :core,:OFF
@18:01:30.87 Gate.power :analytic1,:OFF
@18:01:30.90 Gate.power :analytic2,:OFF
@18:01:30.92 Gate.power :analytic3,:OFF
@18:01:30.94 Gate.power :main,:OFF
@18:01:30.96 Safely stopped and ready to power off
##-- file closed 2023-04-26T19:10:15.986Z