##-- file opened 2023-04-21T14:03:08.730Z @07:03:09.19 -> showStatus @07:03:11.21 -> Cmd.loadCartridge -6 @07:03:11.27 Gate.power :main,:ON,limit to 5000mA @07:03:11.29 Gate.power :analytic1,:ON,limit to 5000mA @07:03:11.32 Gate.power :analytic2,:ON,limit to 5000mA @07:03:11.34 Gate.power :analytic3,:ON,limit to 5000mA @07:03:11.48 Powering core bus @07:03:13.75 SPR Module 1, FW Rev. B, FPGA Rev. C @07:03:14.19 Archive.configure :default @07:03:14.29 Air.configure :default @07:03:14.36 Lysis1.configure :default @07:03:14.42 Lysis2.configure :default @07:03:14.51 Plunge.configure :default @07:03:14.60 SamplePump.configure :default @07:03:14.69 Toroid.configure :default @07:03:14.78 Carousel.configure :default @07:03:14.87 Pressure.configure :default @07:03:14.97 ExtraPump.configure :default @07:03:15.03 PinPort.configure :default @07:03:15.11 All elf controllers running firmware version 4.83 @07:03:15.14 Gate.power :motor,:ON,limit to 5000mA @07:03:15.32 Archive.home.jog 0 @07:03:15.77 PinPort.home.jog 0 @07:03:15.87 Carousel.home.jog 0 @07:03:15.90 Toroid.home.jog 0 @07:03:15.92 Lysis1.home.jog 0 @07:03:15.96 Plunge.home.jog 0 @07:03:16.01 Lysis2.home.jog 0 @07:03:16.03 Air.home.jog 0 @07:03:16.58 Archive.in :homing @07:03:16.86 Archive.jog 1392 @07:03:16.91 Toroid.in :homing @07:03:16.94 Plunge.in :homing @07:03:16.98 Lysis2.in :homing @07:03:17.02 Lysis1.in :homing @07:03:17.06 Air.in :homing @07:03:17.09 PinPort.in :homing @07:03:17.34 Toroid.jog 615 @07:03:17.42 Lysis2.jog 1404 @07:03:17.46 Lysis1.jog 159 @07:03:17.50 Plunge.jog 1549 @07:03:17.53 Air.jog 1374 @07:03:17.56 PinPort.jog -105 @07:03:17.96 PinPort.setRawPosition! 0 @07:03:18.01 PinPort.seek :home @07:03:18.10 Lysis1.setRawPosition! 50 @07:03:18.13 PinPort.in :default @07:03:18.24 Lysis1.seek :home @07:03:18.32 Archive.setRawPosition! 50 @07:03:18.37 Archive.seek :home @07:03:18.51 Lysis1.in :default @07:03:18.65 Archive.in :default @07:03:19.13 Plunge.setRawPosition! 50 @07:03:19.15 Lysis2.setRawPosition! 50 @07:03:19.17 Air.setRawPosition! 50 @07:03:19.27 Plunge.seek :home @07:03:19.35 Air.seek :home @07:03:19.40 Lysis2.seek :home @07:03:19.59 Plunge.in :default @07:03:19.68 Air.in :default @07:03:19.77 Lysis2.in :default @07:03:27.66 I2C::Request::Timeout in Toroid_home -- No Response to I2C::Servo::RelMove3Request[1A:->44] while jogging Toroid Valve (TV) @07:03:27.70 Toroid.in :default @07:03:27.78 I2C::NodeOffline in LRAUV -- Toroid Valve (TV) valve0 Not Responding @07:03:27.80 Retry #1 of 2 @07:03:27.97 Gate.power :motor,:OFF @07:03:28.03 Gate.power :core,:OFF @07:03:28.07 Gate.power :analytic1,:OFF @07:03:28.10 Gate.power :analytic2,:OFF @07:03:28.12 Gate.power :analytic3,:OFF @07:03:28.15 Gate.power :main,:OFF @07:03:31.87 Gate.power :main,:ON,limit to 5000mA @07:03:31.89 Gate.power :analytic1,:ON,limit to 5000mA @07:03:31.92 Gate.power :analytic2,:ON,limit to 5000mA @07:03:31.95 Gate.power :analytic3,:ON,limit to 5000mA @07:03:32.03 Powering core bus @07:03:34.38 SPR Module 1, FW Rev. B, FPGA Rev. C @07:03:34.74 Archive.configure :default @07:03:34.84 Air.configure :default @07:03:34.94 Lysis1.configure :default @07:03:35.01 Lysis2.configure :default @07:03:35.11 Plunge.configure :default @07:03:35.21 SamplePump.configure :default @07:03:35.31 Toroid.configure :default @07:03:35.41 Carousel.configure :default @07:03:35.51 Pressure.configure :default @07:03:35.61 ExtraPump.configure :default @07:03:35.69 PinPort.configure :default @07:03:35.77 All elf controllers running firmware version 4.83 @07:03:35.80 Gate.power :motor,:ON,limit to 5000mA @07:03:36.18 Archive.home.jog 0 @07:03:36.71 Carousel.home.jog 0 @07:03:36.75 Toroid.home.jog 0 @07:03:36.79 Plunge.home.jog 0 @07:03:36.82 Lysis2.home.jog 0 @07:03:36.86 Lysis1.home.jog 0 @07:03:36.91 Air.home.jog 0 @07:03:36.94 PinPort.home.jog 0 @07:03:37.70 Archive.in :homing @07:03:37.92 Archive.jog 1387 @07:03:38.03 Toroid.in :homing @07:03:38.07 Plunge.in :homing @07:03:38.11 Lysis2.in :homing @07:03:38.16 Air.in :homing @07:03:38.21 PinPort.in :homing @07:03:38.25 Lysis1.in :homing @07:03:38.53 Toroid.jog 223 @07:03:38.57 Plunge.jog 385 @07:03:38.61 Lysis2.jog 1405 @07:03:38.64 PinPort.jog -105 @07:03:38.68 Air.jog 1376 @07:03:38.79 Lysis1.jog 157 @07:03:39.16 PinPort.setRawPosition! 0 @07:03:39.23 PinPort.seek :home @07:03:39.33 PinPort.in :default @07:03:39.44 Lysis1.setRawPosition! 50 @07:03:39.54 Archive.setRawPosition! 50 @07:03:39.58 Plunge.setRawPosition! 50 @07:03:39.66 Lysis1.seek :home @07:03:39.73 Archive.seek :home @07:03:39.82 Plunge.seek :home @07:03:40.02 Toroid.setRawPosition! 0 @07:03:40.10 Toroid.seek :home @07:03:40.63 Toroid.in :default @07:03:40.69 Plunge.in :default @07:03:40.73 Lysis2.setRawPosition! 50 @07:03:40.77 Air.setRawPosition! 50 @07:03:40.79 Archive.in :default @07:03:40.83 Lysis1.in :default @07:03:41.12 Lysis2.seek :home @07:03:41.16 Air.seek :home @07:03:41.49 Lysis2.in :default @07:03:41.58 Air.in :default @07:03:41.94 Toroid.seek :clear @07:03:43.55 Carousel.in :default @07:03:43.70 Carousel.home.seek 23 @07:03:43.96 Selecting Cartridge 12 @07:03:44.29 Carousel.seek 12 @07:03:46.82 Lysis1.in :fast @07:03:46.89 Lysis1.seek :locked @07:03:49.48 Plunge.seek :locked @07:03:49.63 Air.in :fast @07:03:49.69 Air.seek :locked @07:03:51.27 Carousel.reconfigure :locked @07:03:51.42 Powering cartridge bus @07:03:54.03 Gate.setIR 1750,5000,0,6 @07:03:54.15 Gate.setIR 0,5000,0,6 @07:03:54.22 Gate.power :cartridge,:OFF @07:03:54.76 Mounted archive Cartridge @07:04:15.85 -> Cmd.startFiltering @07:04:16.11 Preparing to filter cartridge 12 @07:04:16.38 Priming sample loop w/100ml, bypass w/10ml @07:04:16.40 SamplePump.setPosition! 0ml @07:04:16.46 Bypass.open @07:04:17.96 Intake.open @07:04:18.73 Sea 16.2m deep (38.7psia) @07:04:18.74 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:04:21.33 Vacuum tare is 60% of full scale @07:04:21.47 Intake.close @07:04:21.54 Bypass.close @07:04:22.26 Verifying toroid holds at least 0.6..0.85 of 38.7psia @07:04:22.28 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @07:04:26.86 Toroid pressure fell 8% to 35.5psia @07:04:26.87 Bypass.open @07:04:27.95 Intake.open @07:04:29.36 Pressure.in :default @07:04:29.43 Pressure.hold @07:04:29.62 SamplePump.in :prime @07:04:29.70 SamplePump.seek 10ml,59 seconds @07:04:38.47 Pressure.stop @07:04:38.52 SamplePump.setPosition! 0ml @07:04:38.58 Exhaust.open @07:04:40.03 Bypass.close @07:04:41.53 Pressure.in :default @07:04:41.59 Pressure.hold @07:04:41.76 Sea 16.2m deep (38.7psia) @07:04:41.91 SamplePump.in :prime @07:04:41.99 SamplePump.seek 100ml,5:16 @07:06:19.88 Pressure.stop @07:06:19.96 Bypass.open @07:06:20.64 Intake.close @07:06:21.38 Toroid.in :hold @07:06:21.45 Toroid.seek :crack @07:06:23.22 Toroid.seek :cartridge @07:06:24.33 Toroid.in :default @07:06:24.39 Toroid.seek :clear @07:06:26.37 Exhaust.close @07:06:26.41 Bypass.close @07:06:27.17 Verifying cartridge holds at least 0.65..0.85 of 39.4psia @07:06:27.19 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @07:06:31.84 Sampling 1000.0ml in 1:30:34.285714 @07:06:31.85 SamplePump.setPosition! 0ml @07:06:31.92 Exhaust.open @07:06:32.69 Intake.open @07:06:33.50 Pressure.in :default @07:06:33.57 Pressure.hold @07:06:33.69 Sea 16.4m deep (38.9psia) @07:06:33.70 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @07:06:33.86 SamplePump.in :sample @07:06:33.94 SamplePump.seek 1000ml,1:23:51 @07:06:36.53 Cartridge 12 filtering 1/1000ml at 0.38ml/s with 22.5psi vacuum 16.2m deep (38.6psia) @07:07:36.54 Cartridge 12 filtering 25/1000ml at 0.38ml/s with 24.4psi vacuum 14.8m deep (36.5psia) @07:08:36.54 Cartridge 12 filtering 48/1000ml at 0.38ml/s with 25.3psi vacuum 14.1m deep (35.5psia) @07:09:36.94 Cartridge 12 filtering 72/1000ml at 0.39ml/s with 24.0psi vacuum 16.4m deep (38.9psia) @07:10:36.54 Cartridge 12 filtering 95/1000ml at 0.38ml/s with 26.1psi vacuum 14.8m deep (36.6psia) @07:11:36.54 Cartridge 12 filtering 117/1000ml at 0.37ml/s with 22.5psi vacuum 15.9m deep (38.2psia) @07:12:36.54 Cartridge 12 filtering 140/1000ml at 0.38ml/s with 24.9psi vacuum 14.8m deep (36.6psia) @07:13:36.54 Cartridge 12 filtering 162/1000ml at 0.36ml/s with 22.7psi vacuum 17.0m deep (39.8psia) @07:14:36.54 Cartridge 12 filtering 184/1000ml at 0.35ml/s with 24.4psi vacuum 14.9m deep (36.7psia) @07:15:36.54 Cartridge 12 filtering 206/1000ml at 0.34ml/s with 23.1psi vacuum 15.1m deep (37.0psia) @07:16:36.54 Cartridge 12 filtering 227/1000ml at 0.35ml/s with 24.0psi vacuum 14.8m deep (36.6psia) @07:17:36.54 Cartridge 12 filtering 248/1000ml at 0.35ml/s with 23.0psi vacuum 17.3m deep (40.2psia) @07:18:36.54 Cartridge 12 filtering 269/1000ml at 0.36ml/s with 27.0psi vacuum 14.9m deep (36.7psia) @07:19:36.54 Cartridge 12 filtering 290/1000ml at 0.34ml/s with 26.1psi vacuum 13.6m deep (34.8psia) @07:20:36.54 Cartridge 12 filtering 311/1000ml at 0.33ml/s with 24.7psi vacuum 12.9m deep (33.8psia) @07:21:36.59 Cartridge 12 filtering 331/1000ml at 0.32ml/s with 24.4psi vacuum 12.7m deep (33.4psia) @07:22:36.54 Cartridge 12 filtering 351/1000ml at 0.33ml/s with 24.6psi vacuum 14.2m deep (35.7psia) @07:23:36.54 Cartridge 12 filtering 371/1000ml at 0.32ml/s with 26.0psi vacuum 14.4m deep (36.0psia) @07:24:36.54 Cartridge 12 filtering 391/1000ml at 0.32ml/s with 25.7psi vacuum 14.8m deep (36.5psia) @07:25:36.54 Cartridge 12 filtering 410/1000ml at 0.33ml/s with 20.8psi vacuum 15.2m deep (37.1psia) @07:26:36.54 Cartridge 12 filtering 430/1000ml at 0.32ml/s with 21.7psi vacuum 13.3m deep (34.4psia) @07:27:36.54 Cartridge 12 filtering 449/1000ml at 0.33ml/s with 28.0psi vacuum 14.3m deep (35.9psia) @07:28:36.54 Cartridge 12 filtering 468/1000ml at 0.34ml/s with 23.1psi vacuum 17.7m deep (40.8psia) @07:29:36.54 Cartridge 12 filtering 487/1000ml at 0.33ml/s with 27.4psi vacuum 14.4m deep (36.0psia) @07:30:36.54 Cartridge 12 filtering 505/1000ml at 0.30ml/s with 24.0psi vacuum 14.0m deep (35.4psia) @07:31:36.54 Cartridge 12 filtering 524/1000ml at 0.31ml/s with 23.9psi vacuum 15.3m deep (37.3psia) @07:32:36.54 Cartridge 12 filtering 542/1000ml at 0.29ml/s with 26.0psi vacuum 11.3m deep (31.4psia) @07:33:36.54 Cartridge 12 filtering 560/1000ml at 0.29ml/s with 22.1psi vacuum 15.3m deep (37.3psia) @07:34:36.54 Cartridge 12 filtering 578/1000ml at 0.32ml/s with 24.8psi vacuum 16.8m deep (39.6psia) @07:35:36.54 Cartridge 12 filtering 596/1000ml at 0.31ml/s with 28.4psi vacuum 13.7m deep (34.9psia) @07:36:36.54 Cartridge 12 filtering 614/1000ml at 0.30ml/s with 19.6psi vacuum 16.0m deep (38.3psia) @07:37:36.54 Cartridge 12 filtering 631/1000ml at 0.31ml/s with 22.7psi vacuum 18.1m deep (41.4psia) @07:38:36.54 Cartridge 12 filtering 649/1000ml at 0.29ml/s with 25.4psi vacuum 13.5m deep (34.6psia) @07:39:36.54 Cartridge 12 filtering 666/1000ml at 0.27ml/s with 24.5psi vacuum 12.3m deep (32.9psia) @07:40:36.54 Cartridge 12 filtering 683/1000ml at 0.31ml/s with 23.1psi vacuum 16.5m deep (39.1psia) @07:41:36.54 Cartridge 12 filtering 700/1000ml at 0.27ml/s with 23.6psi vacuum 14.1m deep (35.5psia) @07:42:36.54 Cartridge 12 filtering 717/1000ml at 0.27ml/s with 23.2psi vacuum 16.9m deep (39.7psia) @07:43:36.54 Cartridge 12 filtering 734/1000ml at 0.27ml/s with 25.7psi vacuum 11.0m deep (30.9psia) @07:44:36.54 Cartridge 12 filtering 750/1000ml at 0.27ml/s with 23.2psi vacuum 13.5m deep (34.7psia) @07:45:36.54 Cartridge 12 filtering 767/1000ml at 0.28ml/s with 24.3psi vacuum 15.1m deep (37.1psia) @07:46:36.84 Cartridge 12 filtering 783/1000ml at 0.27ml/s with 21.2psi vacuum 16.4m deep (38.9psia) @07:47:36.54 Cartridge 12 filtering 800/1000ml at 0.29ml/s with 21.8psi vacuum 17.4m deep (40.4psia) @07:48:36.54 Cartridge 12 filtering 816/1000ml at 0.27ml/s with 19.6psi vacuum 16.0m deep (38.3psia) @07:49:36.54 Cartridge 12 filtering 832/1000ml at 0.25ml/s with 22.0psi vacuum 13.7m deep (35.0psia) @07:50:36.54 Cartridge 12 filtering 848/1000ml at 0.27ml/s with 25.0psi vacuum 14.8m deep (36.6psia) @07:51:36.54 Cartridge 12 filtering 864/1000ml at 0.26ml/s with 24.9psi vacuum 12.8m deep (33.7psia) @07:52:36.54 Cartridge 12 filtering 879/1000ml at 0.25ml/s with 23.8psi vacuum 14.6m deep (36.2psia) @07:53:36.54 Cartridge 12 filtering 895/1000ml at 0.24ml/s with 24.2psi vacuum 11.6m deep (31.9psia) @07:54:36.54 Cartridge 12 filtering 910/1000ml at 0.26ml/s with 25.7psi vacuum 15.5m deep (37.7psia) @07:55:36.54 Cartridge 12 filtering 926/1000ml at 0.26ml/s with 21.8psi vacuum 12.6m deep (33.3psia) @07:56:36.54 Cartridge 12 filtering 941/1000ml at 0.27ml/s with 24.8psi vacuum 14.2m deep (35.7psia) @07:57:36.54 Cartridge 12 filtering 956/1000ml at 0.25ml/s with 24.1psi vacuum 13.2m deep (34.2psia) @07:58:36.54 Cartridge 12 filtering 971/1000ml at 0.25ml/s with 23.4psi vacuum 15.2m deep (37.2psia) @07:59:36.54 Cartridge 12 filtering 986/1000ml at 0.25ml/s with 21.5psi vacuum 15.6m deep (37.8psia) @08:00:31.85 Pressure.stop @08:00:31.97 Intake.close @08:00:32.01 Exhaust.close @08:00:32.24 Toroid.in :hold @08:00:32.31 Toroid.seek :bypass @08:00:33.93 Toroid.in :default @08:00:34.01 Toroid.seek :clear @08:00:35.62 Sampled 1000.0ml @08:00:37.14 -> Cmd.startProcessing @08:00:37.58 Plunge.seek :reagent @08:00:38.38 Sample loop closed. Ready to Deliver Reagents @08:00:38.40 Archive.seek :sealed @08:00:42.34 Archive.seek :evac @08:00:56.45 delay 1:00 @08:01:56.48 Archive.seek :clear @08:02:14.00 RNAlater soak for 10:00 @08:12:14.03 Archive.seek :tVent @08:12:16.48 delay 1 second @08:12:17.50 Archive.seek :evac @08:12:33.03 delay 10 seconds @08:12:43.06 Sample preserved and evacuated @08:12:43.98 -> Cmd.stop @08:12:44.14 Carousel.in :default @08:12:44.25 Carousel.home.seek 12 @08:12:44.55 Plunge.seek :locked @08:12:45.43 Archive.in :unlocking @08:12:45.56 Archive.seek :clear @08:12:45.61 Air.in :unlocking @08:12:45.78 Air.seek :clear @08:12:45.87 Lysis1.in :unlocking @08:12:46.42 Lysis1.seek :clear @08:12:50.21 Gate.power :motor,:OFF @08:12:50.23 Gate.power :core,:OFF @08:12:50.25 Gate.power :analytic1,:OFF @08:12:50.27 Gate.power :analytic2,:OFF @08:12:50.29 Gate.power :analytic3,:OFF @08:12:50.32 Gate.power :main,:OFF @08:12:50.34 Safely stopped and ready to power off ##-- file closed 2023-04-21T15:24:39.105Z ##-- file opened 2023-04-21T15:24:39.107Z @08:24:39.39 -> showStatus @08:24:41.41 -> Cmd.loadCartridge -10 @08:24:41.47 Gate.power :main,:ON,limit to 5000mA @08:24:41.49 Gate.power :analytic1,:ON,limit to 5000mA @08:24:41.52 Gate.power :analytic2,:ON,limit to 5000mA @08:24:41.54 Gate.power :analytic3,:ON,limit to 5000mA @08:24:41.68 Powering core bus @08:24:43.95 SPR Module 1, FW Rev. B, FPGA Rev. C @08:24:44.38 Archive.configure :default @08:24:44.47 Air.configure :default @08:24:44.53 Lysis1.configure :default @08:24:44.60 Lysis2.configure :default @08:24:44.69 Plunge.configure :default @08:24:44.78 SamplePump.configure :default @08:24:44.87 Toroid.configure :default @08:24:44.96 Carousel.configure :default @08:24:45.05 Pressure.configure :default @08:24:45.14 ExtraPump.configure :default @08:24:45.21 PinPort.configure :default @08:24:45.28 All elf controllers running firmware version 4.83 @08:24:45.31 Gate.power :motor,:ON,limit to 5000mA @08:24:45.44 Archive.home.jog 0 @08:24:46.02 Carousel.home.jog 0 @08:24:46.04 Toroid.home.jog 0 @08:24:46.08 Lysis2.home.jog 0 @08:24:46.14 PinPort.home.jog 0 @08:24:46.17 Plunge.home.jog 0 @08:24:46.20 Air.home.jog 0 @08:24:46.25 Lysis1.home.jog 0 @08:24:46.80 Archive.in :homing @08:24:46.92 Archive.jog 1393 @08:24:47.11 PinPort.in :homing @08:24:47.15 Toroid.in :homing @08:24:47.18 Plunge.in :homing @08:24:47.22 Lysis2.in :homing @08:24:47.25 Lysis1.in :homing @08:24:47.29 Air.in :homing @08:24:47.50 PinPort.jog -105 @08:24:47.54 Toroid.jog 619 @08:24:47.59 Plunge.jog 1534 @08:24:47.62 Lysis2.jog 1407 @08:24:47.67 Air.jog 1378 @08:24:47.73 Lysis1.jog 157 @08:24:47.95 PinPort.setRawPosition! 0 @08:24:48.00 PinPort.seek :home @08:24:48.08 PinPort.in :default @08:24:48.16 Archive.setRawPosition! 50 @08:24:48.21 Archive.seek :home @08:24:48.29 Lysis1.setRawPosition! 50 @08:24:48.35 Lysis1.seek :home @08:24:48.49 Archive.in :default @08:24:49.02 Lysis2.setRawPosition! 50 @08:24:49.05 Air.setRawPosition! 50 @08:24:49.11 Plunge.setRawPosition! 50 @08:24:49.15 Lysis1.in :default @08:24:49.27 Lysis2.seek :home @08:24:49.33 Air.seek :home @08:24:49.39 Plunge.seek :home @08:24:49.63 Lysis2.in :default @08:24:49.72 Air.in :default @08:24:49.82 Plunge.in :default @08:24:49.84 Toroid.setRawPosition! 0 @08:24:49.93 Toroid.seek :home @08:24:50.03 Toroid.in :default @08:24:50.21 Toroid.seek :clear @08:24:51.83 Carousel.in :default @08:24:51.96 Carousel.home.seek 12 @08:24:52.21 Selecting Cartridge 21 @08:24:52.48 Carousel.seek 21 @08:24:54.61 Lysis1.in :fast @08:24:54.68 Lysis1.seek :locked @08:24:57.27 Plunge.seek :locked @08:24:57.41 Air.in :fast @08:24:57.48 Air.seek :locked @08:24:59.08 Carousel.reconfigure :locked @08:24:59.23 Powering cartridge bus @08:25:01.84 Gate.setIR 1750,5000,0,6 @08:25:01.97 Gate.setIR 0,5000,0,6 @08:25:02.03 PuckHeater.configure :default @08:25:02.10 Gate.power :heat,:ON,limit to 5000mA @08:25:02.81 Mounted lysis Cartridge board#Q05038 @08:25:02.84 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:25:18.98 -> Cmd.startFiltering @08:25:19.22 Preparing to filter cartridge 21 @08:25:19.51 Priming sample loop w/100ml, bypass w/10ml @08:25:19.52 SamplePump.setPosition! 0ml @08:25:19.58 Bypass.open @08:25:21.03 Intake.open @08:25:21.79 Sea 16.4m deep (39.0psia) @08:25:21.81 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:25:24.39 Vacuum tare is 60% of full scale @08:25:24.53 Intake.close @08:25:24.58 Bypass.close @08:25:25.30 Verifying toroid holds at least 0.6..0.85 of 39.3psia @08:25:25.33 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @08:25:29.90 Toroid pressure rose 2% to 40.3psia @08:25:29.91 Bypass.open @08:25:30.64 Intake.open @08:25:32.06 Pressure.in :default @08:25:32.13 Pressure.hold @08:25:32.32 SamplePump.in :prime @08:25:32.39 SamplePump.seek 10ml,59 seconds @08:25:41.14 Pressure.stop @08:25:41.20 SamplePump.setPosition! 0ml @08:25:41.26 Exhaust.open @08:25:42.76 Bypass.close @08:25:44.30 Pressure.in :default @08:25:44.36 Pressure.hold @08:25:44.49 Sea 16.0m deep (38.3psia) @08:25:44.63 SamplePump.in :prime @08:25:44.71 SamplePump.seek 100ml,5:16 @08:27:21.86 Pressure.stop @08:27:21.94 Bypass.open @08:27:22.69 Intake.close @08:27:23.48 Toroid.in :hold @08:27:23.54 Toroid.seek :crack @08:27:25.08 Toroid.seek :cartridge @08:27:26.19 Toroid.in :default @08:27:26.25 Toroid.seek :clear @08:27:28.22 Exhaust.close @08:27:28.25 Bypass.close @08:27:29.01 Verifying cartridge holds at least 0.65..0.85 of 38.2psia @08:27:29.03 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @08:27:33.67 Sampling 1000.0ml in 1:30:34.285714 @08:27:33.69 SamplePump.setPosition! 0ml @08:27:33.75 Exhaust.open @08:27:34.51 Intake.open @08:27:35.34 Pressure.in :default @08:27:35.41 Pressure.hold @08:27:35.53 Sea 15.7m deep (37.9psia) @08:27:35.54 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @08:27:35.70 SamplePump.in :sample @08:27:35.77 SamplePump.seek 1000ml,1:23:51 @08:27:38.38 Cartridge 21 filtering 1/1000ml at 0.43ml/s with 25.0psi vacuum 14.4m deep (36.0psia) @08:28:38.38 Cartridge 21 filtering 25/1000ml at 0.41ml/s with 25.0psi vacuum 15.9m deep (38.2psia) @08:29:38.39 Cartridge 21 filtering 48/1000ml at 0.41ml/s with 24.1psi vacuum 17.4m deep (40.4psia) @08:30:38.38 Cartridge 21 filtering 72/1000ml at 0.40ml/s with 24.8psi vacuum 15.1m deep (37.0psia) @08:31:38.39 Cartridge 21 filtering 95/1000ml at 0.40ml/s with 24.3psi vacuum 16.5m deep (39.2psia) @08:32:38.38 Cartridge 21 filtering 117/1000ml at 0.36ml/s with 24.0psi vacuum 14.8m deep (36.5psia) @08:33:38.39 Cartridge 21 filtering 140/1000ml at 0.38ml/s with 25.7psi vacuum 14.8m deep (36.5psia) @08:34:38.38 Cartridge 21 filtering 162/1000ml at 0.36ml/s with 21.5psi vacuum 15.4m deep (37.5psia) @08:35:38.38 Cartridge 21 filtering 184/1000ml at 0.35ml/s with 23.1psi vacuum 14.6m deep (36.2psia) @08:36:38.38 Cartridge 21 filtering 206/1000ml at 0.34ml/s with 24.5psi vacuum 15.0m deep (36.9psia) @08:37:38.38 Cartridge 21 filtering 227/1000ml at 0.35ml/s with 21.3psi vacuum 16.1m deep (38.5psia) @08:38:38.38 Cartridge 21 filtering 248/1000ml at 0.36ml/s with 23.1psi vacuum 15.2m deep (37.1psia) @08:39:38.38 Cartridge 21 filtering 269/1000ml at 0.34ml/s with 26.4psi vacuum 15.4m deep (37.4psia) @08:40:38.38 Cartridge 21 filtering 290/1000ml at 0.36ml/s with 25.0psi vacuum 15.3m deep (37.3psia) @08:41:38.38 Cartridge 21 filtering 310/1000ml at 0.35ml/s with 22.4psi vacuum 15.4m deep (37.4psia) @08:42:38.38 Cartridge 21 filtering 330/1000ml at 0.34ml/s with 25.8psi vacuum 15.6m deep (37.8psia) @08:43:38.39 Cartridge 21 filtering 350/1000ml at 0.32ml/s with 23.6psi vacuum 14.4m deep (35.9psia) @08:44:38.38 Cartridge 21 filtering 370/1000ml at 0.30ml/s with 24.9psi vacuum 13.3m deep (34.3psia) @08:45:38.38 Cartridge 21 filtering 389/1000ml at 0.31ml/s with 26.7psi vacuum 14.5m deep (36.1psia) @08:46:38.38 Cartridge 21 filtering 408/1000ml at 0.32ml/s with 22.0psi vacuum 14.1m deep (35.5psia) @08:47:38.43 Cartridge 21 filtering 427/1000ml at 0.31ml/s with 22.7psi vacuum 15.9m deep (38.2psia) @08:48:38.38 Cartridge 21 filtering 446/1000ml at 0.33ml/s with 23.2psi vacuum 14.1m deep (35.6psia) @08:49:38.38 Cartridge 21 filtering 465/1000ml at 0.30ml/s with 22.1psi vacuum 15.9m deep (38.2psia) @08:50:38.38 Cartridge 21 filtering 483/1000ml at 0.30ml/s with 22.5psi vacuum 14.0m deep (35.5psia) @08:51:38.39 Cartridge 21 filtering 502/1000ml at 0.30ml/s with 25.4psi vacuum 15.0m deep (36.9psia) @08:52:38.38 Cartridge 21 filtering 520/1000ml at 0.29ml/s with 25.3psi vacuum 15.1m deep (37.0psia) @08:53:38.38 Cartridge 21 filtering 538/1000ml at 0.28ml/s with 22.8psi vacuum 14.3m deep (35.8psia) @08:54:38.38 Cartridge 21 filtering 555/1000ml at 0.27ml/s with 25.2psi vacuum 13.4m deep (34.5psia) @08:55:38.39 Cartridge 21 filtering 573/1000ml at 0.28ml/s with 23.6psi vacuum 13.5m deep (34.6psia) @08:56:38.38 Cartridge 21 filtering 590/1000ml at 0.27ml/s with 25.2psi vacuum 13.4m deep (34.5psia) @08:57:38.38 Cartridge 21 filtering 608/1000ml at 0.29ml/s with 23.0psi vacuum 14.7m deep (36.4psia) @08:58:38.38 Cartridge 21 filtering 625/1000ml at 0.27ml/s with 24.4psi vacuum 13.4m deep (34.5psia) @08:59:38.38 Cartridge 21 filtering 642/1000ml at 0.30ml/s with 23.6psi vacuum 15.3m deep (37.3psia) @09:00:38.38 Cartridge 21 filtering 659/1000ml at 0.29ml/s with 25.5psi vacuum 14.7m deep (36.3psia) @09:01:38.39 Cartridge 21 filtering 675/1000ml at 0.29ml/s with 25.3psi vacuum 14.8m deep (36.6psia) @09:02:38.38 Cartridge 21 filtering 692/1000ml at 0.27ml/s with 22.6psi vacuum 16.5m deep (39.2psia) @09:03:38.68 Cartridge 21 filtering 709/1000ml at 0.27ml/s with 23.1psi vacuum 15.1m deep (37.1psia) @09:04:38.38 Cartridge 21 filtering 725/1000ml at 0.26ml/s with 24.3psi vacuum 13.0m deep (34.0psia) @09:05:38.39 Cartridge 21 filtering 741/1000ml at 0.29ml/s with 26.2psi vacuum 15.2m deep (37.1psia) @09:06:38.39 Cartridge 21 filtering 758/1000ml at 0.27ml/s with 24.8psi vacuum 15.2m deep (37.2psia) @09:07:38.39 Cartridge 21 filtering 774/1000ml at 0.28ml/s with 23.2psi vacuum 14.3m deep (35.8psia) @09:08:38.38 Cartridge 21 filtering 790/1000ml at 0.26ml/s with 24.9psi vacuum 14.8m deep (36.5psia) @09:09:38.39 Cartridge 21 filtering 806/1000ml at 0.25ml/s with 23.1psi vacuum 15.0m deep (36.9psia) @09:10:38.39 Cartridge 21 filtering 821/1000ml at 0.27ml/s with 27.0psi vacuum 13.8m deep (35.1psia) @09:11:38.38 Cartridge 21 filtering 837/1000ml at 0.26ml/s with 23.7psi vacuum 14.9m deep (36.6psia) @09:12:38.39 Cartridge 21 filtering 853/1000ml at 0.26ml/s with 23.6psi vacuum 14.0m deep (35.3psia) @09:13:38.39 Cartridge 21 filtering 868/1000ml at 0.26ml/s with 22.7psi vacuum 16.2m deep (38.7psia) @09:14:38.38 Cartridge 21 filtering 884/1000ml at 0.25ml/s with 24.6psi vacuum 12.9m deep (33.7psia) @09:15:38.40 Cartridge 21 filtering 899/1000ml at 0.26ml/s with 24.0psi vacuum 15.3m deep (37.3psia) @09:16:38.39 Cartridge 21 filtering 914/1000ml at 0.26ml/s with 22.2psi vacuum 14.8m deep (36.6psia) @09:17:38.39 Cartridge 21 filtering 929/1000ml at 0.25ml/s with 22.8psi vacuum 15.6m deep (37.8psia) @09:18:38.39 Cartridge 21 filtering 944/1000ml at 0.24ml/s with 23.9psi vacuum 14.9m deep (36.7psia) @09:19:38.39 Cartridge 21 filtering 959/1000ml at 0.25ml/s with 22.5psi vacuum 15.3m deep (37.2psia) @09:20:38.39 Cartridge 21 filtering 974/1000ml at 0.26ml/s with 22.1psi vacuum 16.6m deep (39.2psia) @09:21:38.38 Cartridge 21 filtering 989/1000ml at 0.25ml/s with 24.4psi vacuum 14.4m deep (35.9psia) @09:22:22.14 Pressure.stop @09:22:22.26 Intake.close @09:22:22.30 Exhaust.close @09:22:22.49 Toroid.in :hold @09:22:22.56 Toroid.seek :bypass @09:22:24.19 Toroid.in :default @09:22:24.26 Toroid.seek :clear @09:22:25.87 Sampled 1000.0ml @09:22:27.38 -> Cmd.startProcessing @09:22:28.84 SPR.command \"read_sequences\" @09:22:29.35 SPR.command \"stats_on\" @09:22:29.48 SPR.command \"start 0 1\" @09:22:30.20 SPR.start /var/log/esp/spr23apr2109h.spr.bz2 @09:22:32.20 Handoff.select :waste @09:22:38.89 Syringe tare is 17% of full scale @09:22:38.91 PinPort.in :locking @09:22:38.99 PinPort.seek :locked @09:22:39.46 Archive.in :fast @09:22:39.50 Lysis2.in :fast @09:22:39.59 Lysis1.seek 1350uL @09:22:39.66 Archive.seek :full @09:22:39.76 Lysis2.seek :full @09:22:41.75 Plunge.seek :reagent @09:22:42.63 Evacuating puck with filter bubble point of 50psi @09:22:42.65 PinPort.seek :locked @09:22:42.74 Handoff.select :waste @09:22:49.33 Air syringe pumping up to 1 strokes to reach 8psi @09:22:49.34 Air stroke #1 of 1 @09:22:49.43 Air.in :pressurize @09:22:49.51 Air.seek :pressurized @09:22:55.25 0.1psi after pressurize @09:22:55.26 Air.in :compress @09:22:55.37 Air.seek :evac @09:22:58.40 0.8psi after compress @09:23:08.97 0.1psi after compress (stable) @09:23:08.98 Air.in :default @09:23:09.06 Air.hold @09:23:09.25 at 0.0psi Opened to waste @09:23:09.26 Handoff.select :blocked @09:23:09.40 Archive syringe pumping up to 8 strokes to reach 50psi @09:23:09.41 Archive stroke #1 of 8 @09:23:09.50 Archive.in :pressurize @09:23:09.62 Archive.seek :pressurized @09:23:22.12 8.7psi after pressurize @09:23:38.70 19.3psi after pressurize (stable) @09:23:38.72 Archive.in :compress @09:23:38.80 Archive.seek :empty @09:23:53.44 30.4psi after compress @09:24:04.02 31.4psi after compress (stable) @09:24:04.04 Archive.in :gulp @09:24:04.12 Archive.seek :topVent @09:24:10.37 31.5psi after gulp @09:24:16.16 31.4psi after gulp (stable) @09:24:16.17 Archive stroke #2 of 8 @09:24:16.28 Archive.in :pressurize @09:24:16.35 Archive.seek :pressurized @09:24:28.86 31.3psi after pressurize @09:24:47.44 36.0psi after pressurize (stable) @09:24:47.46 Archive.in :compress @09:24:47.55 Archive.seek :empty @09:25:01.33 Reached 50.2 of 50.0psi after pumping 3441uL (1.99 strokes) @09:25:01.35 Archive.in :default @09:25:01.43 Archive.hold @09:25:01.50 Handoff.select :waste @09:25:14.68 delay 1:00 @09:26:14.70 Handoff.select :blocked @09:26:37.38 Handoff.select :waste @09:26:37.50 1.9psi at evacEnd @09:26:37.51 Handoff.select :blocked @09:26:37.66 Archive.in :fast @09:26:37.73 Archive.seek :topVent @09:26:51.34 Stable @1.2psi after Handoff blocked @09:26:51.36 Archive syringe pumping up to 8 strokes to reach 31psi @09:26:51.38 Archive stroke #1 of 8 @09:26:51.49 Archive.in :pressurize @09:26:51.56 Archive.seek :pressurized @09:27:04.05 2.0psi after pressurize @09:27:04.07 Archive.in :compress @09:27:04.19 Archive.seek :empty @09:27:18.81 8.4psi after compress @09:27:41.88 15.5psi after compress (stable) @09:27:41.91 Archive.in :gulp @09:27:42.02 Archive.seek :topVent @09:27:48.26 16.4psi after gulp @09:27:53.84 16.7psi after gulp (stable) @09:27:53.85 Archive stroke #2 of 8 @09:27:53.96 Archive.in :pressurize @09:27:54.03 Archive.seek :pressurized @09:28:06.53 17.4psi after pressurize @09:28:06.56 Archive.in :compress @09:28:06.65 Archive.seek :empty @09:28:21.33 22.9psi after compress @09:28:45.91 30.0psi after compress (stable) @09:28:45.92 Archive.in :gulp @09:28:46.01 Archive.seek :topVent @09:28:52.26 30.9psi after gulp @09:28:56.07 Reached 31.3 of 31.2psi after pumping 3460uL (2.00 strokes) @09:28:56.09 Archive.in :default @09:28:56.17 Archive.hold @09:28:56.24 Handoff.select :waste @09:29:12.92 delay 1:00 @09:30:12.94 Handoff.select :blocked @09:30:35.63 Handoff.select :waste @09:30:35.75 0.8psi at evacEnd @09:30:35.77 Air.seek :fullVent @09:30:37.20 Archive.in :fast @09:30:37.27 Archive.seek :topVent @09:30:37.35 Seawater evacuated, Pressure above/below filter released. @09:30:37.44 Lysis1.in :slow @09:30:37.51 Lysis1.seek 1050uL @09:31:07.54 delay 5 seconds @09:31:12.56 Handoff.select :blocked @09:31:12.75 Lysis1.seek 450uL @09:32:12.73 delay 3 seconds @09:32:15.81 Air.in mix with 1.40048 parts Lysis1 @09:32:16.27 Air.seek :evac @09:32:16.33 Lysis1.seek 300uL @09:32:31.44 Lysis1.seek :empty @09:33:01.57 Lysis buffer delivered @35.2psi @09:33:01.58 Polling Can every 2:00 @09:33:02.14 PuckHeater.configure :default #ambient 11.5C @09:33:02.19 PuckHeater.seek 80.2C @09:34:14.24 PuckHeater.hold 2:00,80.2C @09:36:14.07 Polling Can every 2:00 @09:36:14.62 PuckHeater.configure :default #ambient 11.6C @09:36:14.69 PuckHeater.hold 30 seconds,79.2C @09:36:44.84 Polling Can every 2:00 @09:36:45.39 PuckHeater.configure :default #ambient 11.5C @09:36:45.44 PuckHeater.hold 30 seconds,78.7C @09:37:15.57 Polling Can every 2:00 @09:37:16.11 PuckHeater.configure :default #ambient 11.5C @09:37:16.17 PuckHeater.hold 10:00,78.1C @09:47:15.19 Lysis complete. Syringe pressure @39.2psi @09:47:15.21 Archive syringe pumping up to 5 strokes to reach 40psi @09:47:15.23 Archive stroke #1 of 5 @09:47:15.33 Archive.in :pressurize @09:47:15.40 Archive.seek :pressurized @09:47:27.90 38.6psi after pressurize @09:47:33.47 38.5psi after pressurize (stable) @09:47:33.49 Archive.in :compress @09:47:33.57 Archive.seek :empty @09:47:40.51 Reached 43.0 of 40.0psi after pumping 1564uL (0.90 strokes) @09:47:40.52 Archive.in :default @09:47:40.60 Archive.hold @09:47:40.67 delay 2 seconds @09:47:42.69 Archive.in :fast @09:47:42.76 Archive.seek :full @09:47:49.35 Lysis1.seek 138uL @09:48:03.24 delay 1 second @09:48:04.26 Archive.seek :full @09:48:04.30 Lysis1.seek :empty @09:48:18.24 Handoff.select :blocked @09:48:18.33 Initial air pressure @52.9psi @09:48:18.40 Lysis1.in :extract @09:48:18.49 Lysis1.seek 600uL,nil @09:48:20.19 Archive syringe pumping up to 6 strokes to reach 25psi @09:48:20.20 Archive stroke #1 of 6 @09:48:20.35 Archive.in :pressurize @09:48:20.42 Archive.seek :pressurized @09:48:32.91 13.9psi after pressurize @09:49:02.20 Archive.in :default @09:49:02.26 Archive.hold @09:49:02.39 Initial 600uL lysate @13.8psi @09:49:02.40 Handoff.select :blocked @09:49:02.51 Initial air pressure @14.2psi @09:49:02.60 Lysis1.seek 825uL,nil @09:49:02.71 Archive syringe pumping up to 6 strokes to reach 17psi @09:49:02.72 Archive stroke #1 of 6 @09:49:02.87 Archive.in :compress @09:49:02.96 Archive.seek :empty @09:49:12.24 Reached 17.1 of 17.0psi after pumping 1614uL (0.93 strokes) @09:49:12.25 Archive.in :default @09:49:12.32 Archive.hold @09:49:13.08 Archive syringe pumping up to 6 strokes to reach 17psi @09:49:13.09 Archive stroke #1 of 6 @09:49:13.19 Archive.in :compress @09:49:13.27 Archive.seek :empty @09:49:15.13 Reached 16.9 of 17.0psi after pumping 1650uL (0.95 strokes) @09:49:15.14 Archive.in :default @09:49:15.23 Archive.hold @09:49:16.01 Archive syringe pumping up to 6 strokes to reach 17psi @09:49:16.02 Archive stroke #1 of 6 @09:49:16.12 Archive.in :compress @09:49:16.19 Archive.seek :empty @09:49:18.37 Archive.in :default @09:49:18.45 Archive.hold @09:49:18.72 Recovered 825uL lysate @15.8psi @09:49:18.75 Air.in :halfSpeed @09:49:18.84 Air.seek :fullVent @09:49:21.04 Puck Top Vented, bottom @5.7psi @09:49:21.05 Handoff.select :waste @09:49:21.18 delay 5 seconds @09:49:26.26 Handoff open to waste, bottom @-0.1psi @09:49:26.28 Air.in :default @09:49:26.37 Air.seek :evac @09:49:27.79 Lysis2.in :DAmix @09:49:27.91 Lysis1.in :DAmix @09:49:28.38 Lysis2.seek 1480uL @09:49:28.42 Lysis1.seek 575uL @09:50:03.60 delay 5 seconds @09:50:08.62 Handoff.select SPR @09:50:08.75 SPR.command \"start 2 14\" @09:50:09.79 SPR stabilized @09:50:09.93 delay 10 seconds @09:50:19.94 SPR.command \"start 1 0\" @09:50:22.66 Waiting up to 4:00 for SPR to enter state 1 @09:52:26.92 SPR.command \"hold_on\" @09:52:28.34 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @09:52:28.82 Lysis2.seek 1180uL @09:52:28.86 Lysis1.seek 275uL @09:53:10.95 delay 15 seconds @09:53:25.97 SPR.command \"hold_off\" @09:53:28.99 Waiting up to 38:00 for SPR to enter state 7 @10:11:28.58 SPRsummary:24RIU,160RIU,178RIU,244RIU,2.76ng/L,none,2.12ng/L after 3104s @10:18:05.80 SPR analysis completed @10:18:11.12 Ended log spr23apr2109h.spr.bz2 @10:18:11.14 SPR.command \"stats_off\" @10:18:13.02 -> Cmd.stop @10:18:13.12 Handoff.select :close @10:18:13.25 Gate.power :heat,:OFF @10:18:13.27 Gate.power :cartridge,:OFF @10:18:13.36 PinPort.in :unlocking @10:18:13.43 PinPort.seek :clear @10:18:13.91 Carousel.in :default @10:18:14.02 Carousel.home.seek 21 @10:18:14.32 Plunge.seek :locked @10:18:15.19 Archive.in :unlocking @10:18:15.33 Archive.seek :clear @10:18:15.37 Air.in :unlocking @10:18:15.54 Air.seek :clear @10:18:15.60 Lysis1.in :unlocking @10:18:15.76 Lysis1.seek :clear @10:18:15.85 Lysis2.in :unlocking @10:18:15.91 Lysis2.seek :clear @10:18:19.92 Gate.power :motor,:OFF @10:18:19.94 Gate.power :core,:OFF @10:18:19.96 Gate.power :analytic1,:OFF @10:18:19.99 Gate.power :analytic2,:OFF @10:18:20.01 Gate.power :analytic3,:OFF @10:18:20.04 Gate.power :main,:OFF @10:18:20.06 Safely stopped and ready to power off ##-- file closed 2023-04-22T16:35:11.027Z ##-- file opened 2023-04-22T16:35:11.032Z @09:35:11.25 -> showStatus @09:35:13.27 -> Cmd.loadCartridge -6 @09:35:13.33 Gate.power :main,:ON,limit to 5000mA @09:35:13.35 Gate.power :analytic1,:ON,limit to 5000mA @09:35:13.38 Gate.power :analytic2,:ON,limit to 5000mA @09:35:13.41 Gate.power :analytic3,:ON,limit to 5000mA @09:35:13.54 Powering core bus @09:35:15.80 SPR Module 1, FW Rev. B, FPGA Rev. C @09:35:16.25 Archive.configure :default @09:35:16.35 Air.configure :default @09:35:16.41 Lysis1.configure :default @09:35:16.48 Lysis2.configure :default @09:35:16.57 Plunge.configure :default @09:35:16.66 SamplePump.configure :default @09:35:16.75 Toroid.configure :default @09:35:16.84 Carousel.configure :default @09:35:16.94 Pressure.configure :default @09:35:17.03 ExtraPump.configure :default @09:35:17.10 PinPort.configure :default @09:35:17.17 All elf controllers running firmware version 4.83 @09:35:17.20 Gate.power :motor,:ON,limit to 5000mA @09:35:17.48 Air.home.jog 0 @09:35:17.68 Archive.home.jog 0 @09:35:18.02 Carousel.home.jog 0 @09:35:18.06 Toroid.home.jog 0 @09:35:18.09 Lysis2.home.jog 0 @09:35:18.13 Lysis1.home.jog 0 @09:35:18.21 PinPort.home.jog 0 @09:35:18.24 Plunge.home.jog 0 @09:35:18.77 Air.in :homing @09:35:18.80 Archive.in :homing @09:35:18.95 Air.jog 1377 @09:35:18.98 Archive.jog 1393 @09:35:19.18 Toroid.in :homing @09:35:19.21 Plunge.in :homing @09:35:19.24 Lysis1.in :homing @09:35:19.29 Lysis2.in :homing @09:35:19.33 PinPort.in :homing @09:35:19.46 Toroid.jog 629 @09:35:19.56 PinPort.jog -106 @09:35:19.57 Plunge.jog 1554 @09:35:19.62 Lysis1.jog 157 @09:35:19.70 Lysis2.jog 1404 @09:35:19.89 PinPort.setRawPosition! 0 @09:35:19.94 PinPort.seek :home @09:35:20.02 PinPort.in :default @09:35:20.15 Air.setRawPosition! 50 @09:35:20.23 Lysis1.setRawPosition! 50 @09:35:20.26 Air.seek :home @09:35:20.28 Archive.setRawPosition! 50 @09:35:20.40 Lysis1.seek :home @09:35:20.80 Archive.seek :home @09:35:20.95 Air.in :default @09:35:21.04 Plunge.setRawPosition! 50 @09:35:21.07 Lysis2.setRawPosition! 50 @09:35:21.16 Plunge.seek :home @09:35:21.19 Lysis2.seek :home @09:35:21.33 Lysis1.in :default @09:35:21.35 Archive.in :default @09:35:21.49 Plunge.in :default @09:35:21.57 Lysis2.in :default @09:35:21.78 Toroid.setRawPosition! 0 @09:35:21.83 Toroid.seek :home @09:35:21.92 Toroid.in :default @09:35:22.00 Toroid.seek :clear @09:35:23.62 Carousel.in :default @09:35:23.74 Carousel.home.seek 21 @09:35:24.00 Selecting Cartridge 10 @09:35:24.32 Carousel.seek 10 @09:35:26.84 Lysis1.in :fast @09:35:26.91 Lysis1.seek :locked @09:35:29.52 Plunge.seek :locked @09:35:29.65 Air.in :fast @09:35:29.73 Air.seek :locked @09:35:31.30 Carousel.reconfigure :locked @09:35:31.45 Powering cartridge bus @09:35:34.07 Gate.setIR 1750,5000,0,6 @09:35:34.19 Gate.setIR 0,5000,0,6 @09:35:34.25 Gate.power :cartridge,:OFF @09:35:34.79 Mounted archive Cartridge @09:35:50.11 -> Cmd.startFiltering @09:35:50.36 Preparing to filter cartridge 10 @09:35:50.68 Priming sample loop w/100ml, bypass w/10ml @09:35:50.70 SamplePump.setPosition! 0ml @09:35:50.76 Bypass.open @09:35:52.24 Intake.open @09:35:52.97 Sea 7.9m deep (26.4psia) @09:35:52.99 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:35:56.07 Vacuum tare is 60% of full scale @09:35:56.21 Pressure.in :default @09:35:56.27 Pressure.hold @09:35:56.46 SamplePump.in :prime @09:35:56.53 SamplePump.seek 10ml,59 seconds @09:36:05.30 Pressure.stop @09:36:05.35 SamplePump.setPosition! 0ml @09:36:05.41 Exhaust.open @09:36:06.92 Bypass.close @09:36:08.46 Pressure.in :default @09:36:08.52 Pressure.hold @09:36:08.65 Sea 7.9m deep (26.3psia) @09:36:08.80 SamplePump.in :prime @09:36:08.87 SamplePump.seek 100ml,5:16 @09:37:47.53 Pressure.stop @09:37:47.61 Bypass.open @09:37:48.33 Intake.close @09:37:49.11 Toroid.in :hold @09:37:49.18 Toroid.seek :crack @09:37:50.72 Toroid.seek :cartridge @09:37:51.82 Toroid.in :default @09:37:51.89 Toroid.seek :clear @09:37:53.85 Pressurizing cartridge to 26psi above 26.5psia @09:37:53.86 SamplePump.setPosition! 0ml @09:37:53.92 Intake.open @09:37:54.58 Bypass.close @09:37:55.29 Exhaust.close @09:37:56.16 Pressure.in :default @09:37:56.24 Pressure.hold @09:37:56.43 SamplePump.in :charge @09:37:56.50 SamplePump.seek 2.3ml,42 seconds @09:38:02.39 Pressure.stop @09:38:02.45 Intake.close @09:38:03.18 Pressurized cartridge with 0.161ml to 26.0psi @09:38:03.19 Isolated @49.3psia (22.8psi) @09:38:03.22 Verifying cartridge holds at least 0.65..0.85 of 49.3psia @09:38:03.25 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:38:07.83 Cartridge pressure fell 4% to 47.4psia @09:38:07.93 Sampling 1000.0ml in 1:25:20 @09:38:07.94 SamplePump.setPosition! 0ml @09:38:08.02 Exhaust.open @09:38:08.81 Intake.open @09:38:09.64 Pressure.in :default @09:38:09.70 Pressure.hold @09:38:09.83 Sea 7.6m deep (25.9psia) @09:38:09.84 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @09:38:10.00 SamplePump.in :sample @09:38:10.13 SamplePump.seek 1000ml,1:23:51 @09:38:12.67 Cartridge 10 filtering 1/1000ml at 0.39ml/s with 20.0psi vacuum 7.3m deep (25.5psia) @09:39:12.67 Cartridge 10 filtering 24/1000ml at 0.40ml/s with 26.8psi vacuum 5.4m deep (22.7psia) @09:40:13.05 Cartridge 10 filtering 47/1000ml at 0.37ml/s with 25.5psi vacuum 5.6m deep (22.9psia) @09:41:12.72 Cartridge 10 filtering 69/1000ml at 0.36ml/s with 24.5psi vacuum 6.5m deep (24.3psia) @09:42:12.68 Cartridge 10 filtering 91/1000ml at 0.38ml/s with 26.3psi vacuum 5.5m deep (22.8psia) @09:43:12.67 Cartridge 10 filtering 113/1000ml at 0.35ml/s with 22.0psi vacuum 7.4m deep (25.6psia) @09:44:12.67 Cartridge 10 filtering 135/1000ml at 0.37ml/s with 23.1psi vacuum 8.3m deep (27.0psia) @09:45:12.67 Cartridge 10 filtering 156/1000ml at 0.34ml/s with 24.6psi vacuum 5.8m deep (23.3psia) @09:46:12.68 Cartridge 10 filtering 177/1000ml at 0.33ml/s with 24.1psi vacuum 5.1m deep (22.3psia) @09:47:12.67 Cartridge 10 filtering 197/1000ml at 0.34ml/s with 21.3psi vacuum 6.3m deep (24.1psia) @09:48:12.68 Cartridge 10 filtering 217/1000ml at 0.35ml/s with 27.0psi vacuum 5.9m deep (23.4psia) @09:49:12.67 Cartridge 10 filtering 237/1000ml at 0.32ml/s with 23.4psi vacuum 5.0m deep (22.0psia) @09:50:12.68 Cartridge 10 filtering 257/1000ml at 0.33ml/s with 24.3psi vacuum 7.0m deep (25.1psia) @09:51:12.67 Cartridge 10 filtering 276/1000ml at 0.33ml/s with 23.6psi vacuum 7.3m deep (25.5psia) @09:52:12.68 Cartridge 10 filtering 295/1000ml at 0.31ml/s with 23.4psi vacuum 7.4m deep (25.7psia) @09:53:12.67 Cartridge 10 filtering 314/1000ml at 0.31ml/s with 24.4psi vacuum 5.9m deep (23.4psia) @09:54:12.68 Cartridge 10 filtering 333/1000ml at 0.30ml/s with 25.8psi vacuum 5.5m deep (22.8psia) @09:55:12.67 Cartridge 10 filtering 351/1000ml at 0.31ml/s with 21.3psi vacuum 5.9m deep (23.4psia) @09:56:12.68 Cartridge 10 filtering 369/1000ml at 0.31ml/s with 20.9psi vacuum 7.3m deep (25.5psia) @09:57:12.67 Cartridge 10 filtering 387/1000ml at 0.29ml/s with 25.3psi vacuum 6.3m deep (24.0psia) @09:58:12.68 Cartridge 10 filtering 405/1000ml at 0.31ml/s with 24.2psi vacuum 5.5m deep (22.8psia) @09:59:12.68 Cartridge 10 filtering 422/1000ml at 0.31ml/s with 24.4psi vacuum 4.9m deep (22.0psia) @10:00:12.68 Cartridge 10 filtering 440/1000ml at 0.28ml/s with 26.4psi vacuum 4.2m deep (21.0psia) @10:01:12.67 Cartridge 10 filtering 457/1000ml at 0.30ml/s with 25.8psi vacuum 6.5m deep (24.2psia) @10:02:12.68 Cartridge 10 filtering 474/1000ml at 0.28ml/s with 23.3psi vacuum 6.2m deep (23.9psia) @10:03:12.67 Cartridge 10 filtering 490/1000ml at 0.28ml/s with 23.4psi vacuum 6.3m deep (24.0psia) @10:04:12.68 Cartridge 10 filtering 507/1000ml at 0.27ml/s with 24.0psi vacuum 4.3m deep (21.1psia) @10:05:12.67 Cartridge 10 filtering 523/1000ml at 0.27ml/s with 26.9psi vacuum 3.9m deep (20.5psia) @10:06:12.68 Cartridge 10 filtering 539/1000ml at 0.28ml/s with 22.3psi vacuum 7.5m deep (25.8psia) @10:07:12.67 Cartridge 10 filtering 555/1000ml at 0.25ml/s with 25.2psi vacuum 5.4m deep (22.7psia) @10:08:12.68 Cartridge 10 filtering 571/1000ml at 0.27ml/s with 26.4psi vacuum 5.8m deep (23.3psia) @10:09:12.67 Cartridge 10 filtering 587/1000ml at 0.25ml/s with 24.4psi vacuum 5.0m deep (22.1psia) @10:10:12.68 Cartridge 10 filtering 602/1000ml at 0.27ml/s with 26.8psi vacuum 5.5m deep (22.9psia) @10:11:12.67 Cartridge 10 filtering 617/1000ml at 0.25ml/s with 22.6psi vacuum 6.5m deep (24.3psia) @10:12:12.68 Cartridge 10 filtering 633/1000ml at 0.27ml/s with 25.0psi vacuum 5.1m deep (22.3psia) @10:13:12.68 Cartridge 10 filtering 648/1000ml at 0.25ml/s with 23.8psi vacuum 6.8m deep (24.8psia) @10:14:12.67 Cartridge 10 filtering 662/1000ml at 0.26ml/s with 24.9psi vacuum 4.9m deep (22.0psia) @10:15:12.68 Cartridge 10 filtering 677/1000ml at 0.24ml/s with 22.4psi vacuum 6.2m deep (23.9psia) @10:16:12.68 Cartridge 10 filtering 691/1000ml at 0.26ml/s with 25.2psi vacuum 4.9m deep (21.9psia) @10:17:12.68 Cartridge 10 filtering 706/1000ml at 0.23ml/s with 24.9psi vacuum 6.0m deep (23.5psia) @10:18:12.68 Cartridge 10 filtering 720/1000ml at 0.25ml/s with 24.8psi vacuum 4.8m deep (21.8psia) @10:19:12.68 Cartridge 10 filtering 734/1000ml at 0.22ml/s with 26.8psi vacuum 4.0m deep (20.6psia) @10:20:12.68 Cartridge 10 filtering 748/1000ml at 0.22ml/s with 25.6psi vacuum 4.6m deep (21.5psia) @10:21:12.68 Cartridge 10 filtering 762/1000ml at 0.24ml/s with 25.0psi vacuum 4.3m deep (21.0psia) @10:22:12.69 Cartridge 10 filtering 775/1000ml at 0.23ml/s with 25.5psi vacuum 5.9m deep (23.3psia) @10:23:12.68 Cartridge 10 filtering 789/1000ml at 0.24ml/s with 23.6psi vacuum 7.8m deep (26.2psia) @10:24:12.68 Cartridge 10 filtering 802/1000ml at 0.22ml/s with 23.7psi vacuum 5.8m deep (23.2psia) @10:25:12.68 Cartridge 10 filtering 816/1000ml at 0.22ml/s with 25.6psi vacuum 6.0m deep (23.6psia) @10:26:12.68 Cartridge 10 filtering 829/1000ml at 0.22ml/s with 26.3psi vacuum 5.6m deep (23.0psia) @10:27:12.68 Cartridge 10 filtering 842/1000ml at 0.21ml/s with 22.9psi vacuum 6.0m deep (23.6psia) @10:28:12.69 Cartridge 10 filtering 855/1000ml at 0.21ml/s with 23.7psi vacuum 7.3m deep (25.5psia) @10:29:12.68 Cartridge 10 filtering 868/1000ml at 0.22ml/s with 27.1psi vacuum 5.2m deep (22.4psia) @10:30:12.68 Cartridge 10 filtering 880/1000ml at 0.20ml/s with 21.8psi vacuum 6.2m deep (23.9psia) @10:31:12.68 Cartridge 10 filtering 893/1000ml at 0.20ml/s with 25.0psi vacuum 4.7m deep (21.6psia) @10:32:12.68 Cartridge 10 filtering 906/1000ml at 0.20ml/s with 22.5psi vacuum 6.6m deep (24.5psia) @10:33:12.67 Cartridge 10 filtering 918/1000ml at 0.20ml/s with 26.2psi vacuum 4.8m deep (21.8psia) @10:34:12.68 Cartridge 10 filtering 930/1000ml at 0.20ml/s with 22.7psi vacuum 5.0m deep (22.1psia) @10:35:12.67 Cartridge 10 filtering 943/1000ml at 0.20ml/s with 25.0psi vacuum 4.9m deep (21.9psia) @10:36:12.68 Cartridge 10 filtering 955/1000ml at 0.22ml/s with 23.6psi vacuum 6.6m deep (24.4psia) @10:37:12.67 Cartridge 10 filtering 967/1000ml at 0.21ml/s with 25.4psi vacuum 3.9m deep (20.5psia) @10:38:12.68 Cartridge 10 filtering 980/1000ml at 0.21ml/s with 22.5psi vacuum 7.1m deep (25.2psia) @10:39:12.67 Cartridge 10 filtering 992/1000ml at 0.21ml/s with 23.8psi vacuum 6.3m deep (24.0psia) @10:39:52.00 Pressure.stop @10:39:52.12 Intake.close @10:39:52.16 Exhaust.close @10:39:52.35 Toroid.in :hold @10:39:52.42 Toroid.seek :bypass @10:39:54.04 Toroid.in :default @10:39:54.12 Toroid.seek :clear @10:39:55.73 Sampled 1000.0ml @10:39:57.16 -> Cmd.startProcessing @10:39:57.58 Plunge.seek :reagent @10:39:58.38 Sample loop closed. Ready to Deliver Reagents @10:39:58.40 Archive.seek :sealed @10:40:02.35 Archive.seek :evac @10:40:16.40 delay 1:00 @10:41:16.43 Archive.seek :clear @10:41:33.93 RNAlater soak for 10:00 @10:51:33.96 Archive.seek :tVent @10:51:36.42 delay 1 second @10:51:37.44 Archive.seek :evac @10:51:52.96 delay 10 seconds @10:52:02.99 Sample preserved and evacuated @10:52:03.97 -> Cmd.stop @10:52:04.11 Carousel.in :default @10:52:04.23 Carousel.home.seek 10 @10:52:04.53 Plunge.seek :locked @10:52:05.41 Archive.in :unlocking @10:52:05.55 Air.in :unlocking @10:52:05.59 Archive.seek :clear @10:52:05.76 Air.seek :clear @10:52:05.81 Lysis1.in :unlocking @10:52:05.97 Lysis1.seek :clear @10:52:10.16 Gate.power :motor,:OFF @10:52:10.17 Gate.power :core,:OFF @10:52:10.20 Gate.power :analytic1,:OFF @10:52:10.22 Gate.power :analytic2,:OFF @10:52:10.25 Gate.power :analytic3,:OFF @10:52:10.28 Gate.power :main,:OFF @10:52:10.29 Safely stopped and ready to power off ##-- file closed 2023-04-22T18:04:41.728Z ##-- file opened 2023-04-22T18:04:41.731Z @11:04:42.00 -> showStatus @11:04:44.02 -> Cmd.loadCartridge -10 @11:04:44.09 Gate.power :main,:ON,limit to 5000mA @11:04:44.11 Gate.power :analytic1,:ON,limit to 5000mA @11:04:44.13 Gate.power :analytic2,:ON,limit to 5000mA @11:04:44.16 Gate.power :analytic3,:ON,limit to 5000mA @11:04:44.29 Powering core bus @11:04:46.56 SPR Module 1, FW Rev. B, FPGA Rev. C @11:04:46.99 Archive.configure :default @11:04:47.08 Air.configure :default @11:04:47.15 Lysis1.configure :default @11:04:47.27 Lysis2.configure :default @11:04:47.36 Plunge.configure :default @11:04:47.45 SamplePump.configure :default @11:04:47.54 Toroid.configure :default @11:04:47.63 Carousel.configure :default @11:04:47.73 Pressure.configure :default @11:04:47.82 ExtraPump.configure :default @11:04:47.89 PinPort.configure :default @11:04:47.96 All elf controllers running firmware version 4.83 @11:04:47.99 Gate.power :motor,:ON,limit to 5000mA @11:04:48.29 Lysis1.home.jog 0 @11:04:48.53 Archive.home.jog 0 @11:04:48.64 Air.home.jog 0 @11:04:48.91 Carousel.home.jog 0 @11:04:48.95 Toroid.home.jog 0 @11:04:49.02 Lysis2.home.jog 0 @11:04:49.09 PinPort.home.jog 0 @11:04:49.13 Plunge.home.jog 0 @11:04:49.53 Air.in :homing @11:04:49.60 Archive.in :homing @11:04:49.64 Lysis1.in :homing @11:04:49.78 Air.jog 1383 @11:04:49.85 Lysis1.jog 157 @11:04:49.89 Archive.jog 1393 @11:04:50.10 Plunge.in :homing @11:04:50.12 PinPort.in :homing @11:04:50.15 Toroid.in :homing @11:04:50.19 Lysis2.in :homing @11:04:50.35 PinPort.jog -105 @11:04:50.40 Toroid.jog 620 @11:04:50.44 Lysis2.jog 1406 @11:04:50.49 Plunge.jog 1561 @11:04:50.68 Lysis1.setRawPosition! 50 @11:04:50.72 Lysis1.seek :home @11:04:50.80 PinPort.setRawPosition! 0 @11:04:50.85 PinPort.seek :home @11:04:50.94 PinPort.in :default @11:04:51.03 Air.setRawPosition! 50 @11:04:51.07 Lysis1.in :default @11:04:51.43 Air.seek :home @11:04:51.63 Archive.setRawPosition! 50 @11:04:51.68 Archive.seek :home @11:04:51.78 Lysis2.setRawPosition! 50 @11:04:51.83 Lysis2.seek :home @11:04:51.93 Air.in :default @11:04:51.96 Plunge.setRawPosition! 50 @11:04:52.05 Plunge.seek :home @11:04:52.16 Archive.in :default @11:04:52.19 Lysis2.in :default @11:04:52.34 Plunge.in :default @11:04:52.66 Toroid.setRawPosition! 0 @11:04:52.71 Toroid.seek :home @11:04:52.80 Toroid.in :default @11:04:52.88 Toroid.seek :clear @11:04:54.49 Carousel.in :default @11:04:54.62 Carousel.home.seek 10 @11:04:54.88 Selecting Cartridge 19 @11:04:55.15 Carousel.seek 19 @11:04:57.27 Lysis1.in :fast @11:04:57.34 Lysis1.seek :locked @11:04:59.94 Plunge.seek :locked @11:05:00.09 Air.in :fast @11:05:00.16 Air.seek :locked @11:05:01.73 Carousel.reconfigure :locked @11:05:01.87 Powering cartridge bus @11:05:04.51 Gate.setIR 1750,5000,0,6 @11:05:04.64 Gate.setIR 0,5000,0,6 @11:05:04.71 PuckHeater.configure :default @11:05:04.77 Gate.power :heat,:ON,limit to 5000mA @11:05:05.46 Mounted lysis Cartridge board#Q05075 @11:05:05.49 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:05:21.66 -> Cmd.startFiltering @11:05:21.91 Preparing to filter cartridge 19 @11:05:22.18 Priming sample loop w/100ml, bypass w/10ml @11:05:22.20 SamplePump.setPosition! 0ml @11:05:22.26 Bypass.open @11:05:23.81 Intake.open @11:05:24.53 Sea 7.9m deep (26.3psia) @11:05:24.55 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:05:27.13 Vacuum tare is 60% of full scale @11:05:27.26 Pressure.in :default @11:05:27.32 Pressure.hold @11:05:27.51 SamplePump.in :prime @11:05:27.59 SamplePump.seek 10ml,59 seconds @11:05:36.71 Pressure.stop @11:05:36.77 SamplePump.setPosition! 0ml @11:05:36.83 Exhaust.open @11:05:38.33 Bypass.close @11:05:39.88 Pressure.in :default @11:05:39.95 Pressure.hold @11:05:40.07 Sea 7.5m deep (25.8psia) @11:05:40.21 SamplePump.in :prime @11:05:40.29 SamplePump.seek 100ml,5:16 @11:07:17.67 Pressure.stop @11:07:17.75 Bypass.open @11:07:18.50 Intake.close @11:07:19.28 Toroid.in :hold @11:07:19.35 Toroid.seek :crack @11:07:20.88 Toroid.seek :cartridge @11:07:21.99 Toroid.in :default @11:07:22.06 Toroid.seek :clear @11:07:24.03 Pressurizing cartridge to 26psi above 26.1psia @11:07:24.04 SamplePump.setPosition! 0ml @11:07:24.10 Intake.open @11:07:24.77 Bypass.close @11:07:25.47 Exhaust.close @11:07:26.34 Pressure.in :default @11:07:26.41 Pressure.hold @11:07:26.60 SamplePump.in :charge @11:07:26.67 SamplePump.seek 2.3ml,42 seconds @11:07:37.08 Pressure.stop @11:07:37.14 Intake.close @11:07:37.90 Pressurized cartridge with 0.514ml to 26.0psi @11:07:37.91 Isolated @50.7psia (24.6psi) @11:07:37.94 Verifying cartridge holds at least 0.65..0.85 of 50.7psia @11:07:37.97 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:07:42.64 Sampling 1000.0ml in 1:25:20 @11:07:42.66 SamplePump.setPosition! 0ml @11:07:42.72 Exhaust.open @11:07:43.49 Intake.open @11:07:44.32 Pressure.in :default @11:07:44.38 Pressure.hold @11:07:44.50 Sea 8.6m deep (27.4psia) @11:07:44.51 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @11:07:44.67 SamplePump.in :sample @11:07:44.74 SamplePump.seek 1000ml,1:23:51 @11:07:47.73 Cartridge 19 filtering 1/1000ml at 0.42ml/s with 23.7psi vacuum 7.0m deep (25.0psia) @11:08:47.36 Cartridge 19 filtering 25/1000ml at 0.39ml/s with 23.6psi vacuum 5.8m deep (23.3psia) @11:09:47.36 Cartridge 19 filtering 49/1000ml at 0.38ml/s with 24.1psi vacuum 5.5m deep (22.8psia) @11:10:47.36 Cartridge 19 filtering 73/1000ml at 0.38ml/s with 27.1psi vacuum 5.3m deep (22.5psia) @11:11:47.36 Cartridge 19 filtering 96/1000ml at 0.37ml/s with 27.0psi vacuum 5.2m deep (22.4psia) @11:12:47.36 Cartridge 19 filtering 118/1000ml at 0.39ml/s with 23.9psi vacuum 7.1m deep (25.2psia) @11:13:47.35 Cartridge 19 filtering 140/1000ml at 0.36ml/s with 26.8psi vacuum 5.1m deep (22.2psia) @11:14:47.36 Cartridge 19 filtering 162/1000ml at 0.34ml/s with 24.5psi vacuum 6.5m deep (24.3psia) @11:15:47.35 Cartridge 19 filtering 183/1000ml at 0.35ml/s with 22.7psi vacuum 5.9m deep (23.4psia) @11:16:47.36 Cartridge 19 filtering 204/1000ml at 0.33ml/s with 23.5psi vacuum 6.1m deep (23.7psia) @11:17:47.36 Cartridge 19 filtering 225/1000ml at 0.33ml/s with 24.1psi vacuum 6.7m deep (24.6psia) @11:18:47.35 Cartridge 19 filtering 245/1000ml at 0.35ml/s with 24.1psi vacuum 6.2m deep (23.9psia) @11:19:47.36 Cartridge 19 filtering 265/1000ml at 0.34ml/s with 22.3psi vacuum 6.6m deep (24.5psia) @11:20:47.36 Cartridge 19 filtering 285/1000ml at 0.33ml/s with 23.1psi vacuum 6.0m deep (23.5psia) @11:21:47.36 Cartridge 19 filtering 305/1000ml at 0.33ml/s with 22.6psi vacuum 7.2m deep (25.3psia) @11:22:47.36 Cartridge 19 filtering 324/1000ml at 0.33ml/s with 23.0psi vacuum 6.8m deep (24.7psia) @11:23:47.39 Cartridge 19 filtering 343/1000ml at 0.32ml/s with 24.1psi vacuum 8.3m deep (26.9psia) @11:24:47.36 Cartridge 19 filtering 361/1000ml at 0.30ml/s with 24.9psi vacuum 7.1m deep (25.2psia) @11:25:47.36 Cartridge 19 filtering 379/1000ml at 0.29ml/s with 23.1psi vacuum 6.5m deep (24.4psia) @11:26:47.36 Cartridge 19 filtering 397/1000ml at 0.31ml/s with 25.1psi vacuum 5.4m deep (22.6psia) @11:27:47.36 Cartridge 19 filtering 415/1000ml at 0.27ml/s with 25.0psi vacuum 5.8m deep (23.3psia) @11:28:47.35 Cartridge 19 filtering 432/1000ml at 0.29ml/s with 23.5psi vacuum 5.8m deep (23.2psia) @11:29:47.36 Cartridge 19 filtering 449/1000ml at 0.27ml/s with 23.3psi vacuum 6.7m deep (24.6psia) @11:30:47.36 Cartridge 19 filtering 466/1000ml at 0.26ml/s with 24.2psi vacuum 5.8m deep (23.3psia) @11:31:47.36 Cartridge 19 filtering 482/1000ml at 0.26ml/s with 21.1psi vacuum 7.6m deep (25.9psia) @11:32:47.41 Cartridge 19 filtering 499/1000ml at 0.29ml/s with 23.5psi vacuum 7.3m deep (25.5psia) @11:33:47.35 Cartridge 19 filtering 515/1000ml at 0.28ml/s with 23.7psi vacuum 5.4m deep (22.7psia) @11:34:47.36 Cartridge 19 filtering 531/1000ml at 0.27ml/s with 23.6psi vacuum 6.6m deep (24.4psia) @11:35:47.36 Cartridge 19 filtering 547/1000ml at 0.25ml/s with 24.2psi vacuum 5.6m deep (22.9psia) @11:36:47.36 Cartridge 19 filtering 562/1000ml at 0.26ml/s with 24.2psi vacuum 6.5m deep (24.3psia) @11:37:47.36 Cartridge 19 filtering 578/1000ml at 0.25ml/s with 22.9psi vacuum 7.8m deep (26.2psia) @11:38:47.36 Cartridge 19 filtering 593/1000ml at 0.24ml/s with 23.8psi vacuum 5.4m deep (22.7psia) @11:39:47.36 Cartridge 19 filtering 608/1000ml at 0.26ml/s with 25.2psi vacuum 6.7m deep (24.5psia) @11:40:47.36 Cartridge 19 filtering 623/1000ml at 0.23ml/s with 23.6psi vacuum 6.1m deep (23.6psia) @11:41:47.36 Cartridge 19 filtering 638/1000ml at 0.25ml/s with 26.1psi vacuum 6.4m deep (24.2psia) @11:42:47.36 Cartridge 19 filtering 652/1000ml at 0.24ml/s with 23.6psi vacuum 5.9m deep (23.4psia) @11:43:47.36 Cartridge 19 filtering 667/1000ml at 0.23ml/s with 25.3psi vacuum 6.2m deep (23.8psia) @11:44:47.67 Cartridge 19 filtering 681/1000ml at 0.23ml/s with 23.6psi vacuum 6.3m deep (23.9psia) @11:45:47.36 Cartridge 19 filtering 695/1000ml at 0.23ml/s with 23.8psi vacuum 5.8m deep (23.2psia) @11:46:47.36 Cartridge 19 filtering 709/1000ml at 0.23ml/s with 25.8psi vacuum 6.7m deep (24.7psia) @11:47:47.36 Cartridge 19 filtering 724/1000ml at 0.22ml/s with 23.2psi vacuum 5.7m deep (23.1psia) @11:48:47.36 Cartridge 19 filtering 737/1000ml at 0.22ml/s with 22.7psi vacuum 6.2m deep (23.9psia) @11:49:47.36 Cartridge 19 filtering 751/1000ml at 0.23ml/s with 24.3psi vacuum 7.3m deep (25.5psia) @11:50:47.36 Cartridge 19 filtering 765/1000ml at 0.22ml/s with 24.7psi vacuum 7.2m deep (25.4psia) @11:51:47.36 Cartridge 19 filtering 778/1000ml at 0.24ml/s with 25.6psi vacuum 5.5m deep (22.8psia) @11:52:47.36 Cartridge 19 filtering 792/1000ml at 0.21ml/s with 23.0psi vacuum 6.7m deep (24.5psia) @11:53:47.35 Cartridge 19 filtering 805/1000ml at 0.20ml/s with 25.0psi vacuum 5.5m deep (22.8psia) @11:54:47.36 Cartridge 19 filtering 818/1000ml at 0.22ml/s with 25.4psi vacuum 6.0m deep (23.5psia) @11:55:47.36 Cartridge 19 filtering 831/1000ml at 0.20ml/s with 24.6psi vacuum 5.7m deep (23.1psia) @11:56:47.36 Cartridge 19 filtering 844/1000ml at 0.21ml/s with 23.7psi vacuum 6.1m deep (23.7psia) @11:57:47.36 Cartridge 19 filtering 857/1000ml at 0.22ml/s with 24.4psi vacuum 6.3m deep (24.0psia) @11:58:47.36 Cartridge 19 filtering 870/1000ml at 0.20ml/s with 24.2psi vacuum 6.0m deep (23.5psia) @11:59:47.36 Cartridge 19 filtering 882/1000ml at 0.20ml/s with 25.0psi vacuum 6.0m deep (23.6psia) @12:00:47.36 Cartridge 19 filtering 895/1000ml at 0.21ml/s with 24.1psi vacuum 5.8m deep (23.3psia) @12:01:47.36 Cartridge 19 filtering 907/1000ml at 0.20ml/s with 25.0psi vacuum 6.5m deep (24.3psia) @12:02:47.36 Cartridge 19 filtering 920/1000ml at 0.20ml/s with 25.6psi vacuum 5.3m deep (22.6psia) @12:03:47.36 Cartridge 19 filtering 932/1000ml at 0.21ml/s with 24.8psi vacuum 6.6m deep (24.5psia) @12:04:47.36 Cartridge 19 filtering 944/1000ml at 0.21ml/s with 25.3psi vacuum 6.3m deep (24.0psia) @12:05:47.36 Cartridge 19 filtering 956/1000ml at 0.20ml/s with 24.0psi vacuum 5.6m deep (23.0psia) @12:06:47.36 Cartridge 19 filtering 968/1000ml at 0.20ml/s with 25.7psi vacuum 6.3m deep (24.0psia) @12:07:47.36 Cartridge 19 filtering 981/1000ml at 0.20ml/s with 24.1psi vacuum 5.9m deep (23.4psia) @12:08:47.36 Cartridge 19 filtering 993/1000ml at 0.20ml/s with 25.3psi vacuum 6.3m deep (24.0psia) @12:09:23.94 Pressure.stop @12:09:24.06 Intake.close @12:09:24.13 Exhaust.close @12:09:24.31 Toroid.in :hold @12:09:24.38 Toroid.seek :bypass @12:09:26.00 Toroid.in :default @12:09:26.07 Toroid.seek :clear @12:09:27.68 Sampled 1000.0ml @12:09:29.15 -> Cmd.startProcessing @12:09:30.60 SPR.command \"read_sequences\" @12:09:30.97 SPR.command \"stats_on\" @12:09:31.10 SPR.command \"start 0 1\" @12:09:31.82 SPR.start /var/log/esp/spr23apr2212h.spr.bz2 @12:09:34.31 Handoff.select :waste @12:09:41.00 Syringe tare is 17% of full scale @12:09:41.02 PinPort.in :locking @12:09:41.10 PinPort.seek :locked @12:09:41.58 Archive.in :fast @12:09:41.63 Lysis2.in :fast @12:09:41.71 Lysis1.seek 1350uL @12:09:41.79 Archive.seek :full @12:09:41.88 Lysis2.seek :full @12:09:43.89 Plunge.seek :reagent @12:09:44.81 Evacuating puck with filter bubble point of 50psi @12:09:44.83 PinPort.seek :locked @12:09:44.93 Handoff.select :waste @12:09:51.53 Air syringe pumping up to 1 strokes to reach 8psi @12:09:51.54 Air stroke #1 of 1 @12:09:51.64 Air.in :pressurize @12:09:51.70 Air.seek :pressurized @12:09:57.44 0.1psi after pressurize @12:09:57.46 Air.in :compress @12:09:57.54 Air.seek :evac @12:10:00.54 0.8psi after compress @12:10:11.12 0.2psi after compress (stable) @12:10:11.14 Air.in :default @12:10:11.24 Air.hold @12:10:11.43 at 0.1psi Opened to waste @12:10:11.44 Handoff.select :blocked @12:10:11.58 Archive syringe pumping up to 8 strokes to reach 50psi @12:10:11.59 Archive stroke #1 of 8 @12:10:11.68 Archive.in :pressurize @12:10:11.76 Archive.seek :pressurized @12:10:24.32 9.6psi after pressurize @12:10:51.39 23.6psi after pressurize (stable) @12:10:51.41 Archive.in :compress @12:10:51.49 Archive.seek :empty @12:11:06.12 34.9psi after compress @12:11:18.19 37.5psi after compress (stable) @12:11:18.21 Archive.in :gulp @12:11:18.31 Archive.seek :topVent @12:11:24.58 37.6psi after gulp @12:11:30.16 37.6psi after gulp (stable) @12:11:30.17 Archive stroke #2 of 8 @12:11:30.27 Archive.in :pressurize @12:11:30.34 Archive.seek :pressurized @12:11:42.84 35.5psi after pressurize @12:11:56.42 41.3psi after pressurize (stable) @12:11:56.44 Archive.in :compress @12:11:56.52 Archive.seek :empty @12:12:05.38 Reached 50.1 of 50.0psi after pumping 3335uL (1.93 strokes) @12:12:05.39 Archive.in :default @12:12:05.48 Archive.hold @12:12:05.55 Handoff.select :waste @12:12:14.80 delay 1:00 @12:13:14.82 Handoff.select :blocked @12:13:37.52 Handoff.select :waste @12:13:37.64 0.8psi at evacEnd @12:13:37.65 Handoff.select :blocked @12:13:37.79 Archive.in :fast @12:13:37.85 Archive.seek :topVent @12:13:51.14 Stable @1.5psi after Handoff blocked @12:13:51.17 Archive syringe pumping up to 8 strokes to reach 31psi @12:13:51.18 Archive stroke #1 of 8 @12:13:51.29 Archive.in :pressurize @12:13:51.36 Archive.seek :pressurized @12:14:03.90 2.2psi after pressurize @12:14:03.92 Archive.in :compress @12:14:04.00 Archive.seek :empty @12:14:18.63 10.8psi after compress @12:14:54.21 27.5psi after compress (stable) @12:14:54.23 Archive.in :gulp @12:14:54.31 Archive.seek :topVent @12:15:00.55 28.6psi after gulp @12:15:06.15 29.1psi after gulp (stable) @12:15:06.16 Archive stroke #2 of 8 @12:15:06.28 Archive.in :pressurize @12:15:06.37 Archive.seek :pressurized @12:15:18.87 29.7psi after pressurize @12:15:18.89 Archive.in :compress @12:15:18.97 Archive.seek :empty @12:15:28.70 Reached 31.5 of 31.5psi after pumping 3354uL (1.94 strokes) @12:15:28.72 Archive.in :default @12:15:28.80 Archive.hold @12:15:28.87 Handoff.select :waste @12:15:42.06 delay 1:00 @12:16:42.07 Handoff.select :blocked @12:17:04.76 Handoff.select :waste @12:17:04.88 2.6psi at evacEnd @12:17:04.90 Air.seek :fullVent @12:17:06.33 Archive.in :fast @12:17:06.42 Archive.seek :topVent @12:17:13.17 Seawater evacuated, Pressure above/below filter released. @12:17:13.26 Lysis1.in :slow @12:17:13.33 Lysis1.seek 1050uL @12:17:43.39 delay 5 seconds @12:17:48.41 Handoff.select :blocked @12:17:48.60 Lysis1.seek 450uL @12:18:48.63 delay 3 seconds @12:18:51.71 Air.in mix with 1.40048 parts Lysis1 @12:18:52.18 Air.seek :evac @12:18:52.25 Lysis1.seek 300uL @12:19:07.38 Lysis1.seek :empty @12:19:37.52 Lysis buffer delivered @22.6psi @12:19:37.53 Polling Can every 2:00 @12:19:38.08 PuckHeater.configure :default #ambient 11.9C @12:19:38.13 PuckHeater.seek 80.2C @12:20:51.04 PuckHeater.hold 2:00,80.2C @12:22:50.51 Polling Can every 2:00 @12:22:51.05 PuckHeater.configure :default #ambient 12.0C @12:22:51.10 PuckHeater.hold 30 seconds,79.2C @12:23:21.15 Polling Can every 2:00 @12:23:21.69 PuckHeater.configure :default #ambient 12.0C @12:23:21.75 PuckHeater.hold 30 seconds,78.6C @12:23:51.80 Polling Can every 2:00 @12:23:52.34 PuckHeater.configure :default #ambient 11.9C @12:23:52.40 PuckHeater.hold 10:00,78.1C @12:33:49.57 Lysis complete. Syringe pressure @21.4psi @12:33:49.59 Archive syringe pumping up to 5 strokes to reach 40psi @12:33:49.61 Archive stroke #1 of 5 @12:33:49.71 Archive.in :pressurize @12:33:49.78 Archive.seek :pressurized @12:34:02.31 21.5psi after pressurize @12:34:11.38 38.9psi after pressurize (stable) @12:34:11.40 Archive.in :compress @12:34:11.48 Archive.seek :empty @12:34:13.17 Reached 40.5 of 40.0psi after pumping 1451uL (0.84 strokes) @12:34:13.18 Archive.in :default @12:34:13.26 Archive.hold @12:34:13.33 delay 2 seconds @12:34:15.35 Archive.in :fast @12:34:15.42 Archive.seek :full @12:34:21.65 Lysis1.seek 138uL @12:34:35.56 delay 1 second @12:34:36.58 Archive.seek :full @12:34:36.62 Lysis1.seek :empty @12:34:50.90 Handoff.select :blocked @12:34:51.06 Initial air pressure @40.4psi @12:34:51.12 Lysis1.in :extract @12:34:51.21 Lysis1.seek 600uL,nil @12:34:54.15 Archive syringe pumping up to 6 strokes to reach 25psi @12:34:54.16 Archive stroke #1 of 6 @12:34:54.29 Archive.in :pressurize @12:34:54.36 Archive.seek :pressurized @12:35:06.90 15.4psi after pressurize @12:35:11.09 Archive.in :default @12:35:11.15 Archive.hold @12:35:11.30 Initial 600uL lysate @15.5psi @12:35:11.32 Handoff.select :blocked @12:35:11.43 Initial air pressure @16.2psi @12:35:11.51 Lysis1.seek 825uL,nil @12:35:12.71 Archive syringe pumping up to 6 strokes to reach 17psi @12:35:12.72 Archive stroke #1 of 6 @12:35:12.82 Archive.in :compress @12:35:12.90 Archive.seek :empty @12:35:24.86 Archive.in :default @12:35:24.92 Archive.hold @12:35:25.15 Recovered 825uL lysate @13.9psi @12:35:25.17 Air.in :halfSpeed @12:35:25.25 Air.seek :fullVent @12:35:27.45 Puck Top Vented, bottom @3.1psi @12:35:27.47 Handoff.select :waste @12:35:27.61 delay 5 seconds @12:35:32.67 Handoff open to waste, bottom @-0.1psi @12:35:32.69 Air.in :default @12:35:32.77 Air.seek :evac @12:35:34.19 Lysis2.in :DAmix @12:35:34.31 Lysis1.in :DAmix @12:35:34.75 Lysis2.seek 1480uL @12:35:34.81 Lysis1.seek 575uL @12:36:09.95 delay 5 seconds @12:36:14.97 Handoff.select SPR @12:36:15.09 SPR.command \"start 2 14\" @12:36:16.06 SPR stabilized @12:36:16.20 delay 10 seconds @12:36:26.22 SPR.command \"start 1 0\" @12:36:29.43 Waiting up to 4:00 for SPR to enter state 1 @12:38:33.69 SPR.command \"hold_on\" @12:38:35.59 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @12:38:36.05 Lysis2.seek 1180uL @12:38:36.11 Lysis1.seek 275uL @12:39:18.17 delay 15 seconds @12:39:33.18 SPR.command \"hold_off\" @12:39:35.76 Waiting up to 38:00 for SPR to enter state 7 @12:57:34.39 SPRsummary:28RIU,105RIU,136RIU,165RIU,11ng/L,4.78ng/L,9.57ng/L after 3045s @13:04:11.61 SPR analysis completed @13:04:16.44 Ended log spr23apr2212h.spr.bz2 @13:04:16.45 SPR.command \"stats_off\" @13:04:17.81 -> Cmd.stop @13:04:17.91 Handoff.select :close @13:04:18.04 Gate.power :heat,:OFF @13:04:18.06 Gate.power :cartridge,:OFF @13:04:18.15 PinPort.in :unlocking @13:04:18.22 PinPort.seek :clear @13:04:18.70 Carousel.in :default @13:04:18.82 Carousel.home.seek 19 @13:04:19.13 Plunge.seek :locked @13:04:20.00 Archive.in :unlocking @13:04:20.14 Archive.seek :clear @13:04:20.18 Air.in :unlocking @13:04:20.37 Lysis1.in :unlocking @13:04:20.41 Air.seek :clear @13:04:20.59 Lysis1.seek :clear @13:04:20.68 Lysis2.in :unlocking @13:04:20.75 Lysis2.seek :clear @13:04:24.70 Gate.power :motor,:OFF @13:04:24.72 Gate.power :core,:OFF @13:04:24.75 Gate.power :analytic1,:OFF @13:04:24.77 Gate.power :analytic2,:OFF @13:04:24.79 Gate.power :analytic3,:OFF @13:04:24.82 Gate.power :main,:OFF @13:04:24.84 Safely stopped and ready to power off ##-- file closed 2023-04-23T03:57:52.778Z