##-- file opened 2023-04-20T21:16:17.888Z @14:16:18.77 -> showStatus @14:16:20.38 -> Cmd.loadCartridge -6 @14:16:20.45 Gate.power :main,:ON,limit to 5000mA @14:16:20.47 Gate.power :analytic1,:ON,limit to 5000mA @14:16:20.49 Gate.power :analytic2,:ON,limit to 5000mA @14:16:20.52 Gate.power :analytic3,:ON,limit to 5000mA @14:16:20.65 Powering core bus @14:16:22.92 SPR Module 1, FW Rev. B, FPGA Rev. C @14:16:23.35 Archive.configure :default @14:16:23.44 Air.configure :default @14:16:23.51 Lysis1.configure :default @14:16:23.57 Lysis2.configure :default @14:16:23.66 Plunge.configure :default @14:16:23.75 SamplePump.configure :default @14:16:23.84 Toroid.configure :default @14:16:23.93 Carousel.configure :default @14:16:24.02 Pressure.configure :default @14:16:24.12 ExtraPump.configure :default @14:16:24.18 PinPort.configure :default @14:16:24.25 All elf controllers running firmware version 4.83 @14:16:24.28 Gate.power :motor,:ON,limit to 5000mA @14:16:24.59 Air.home.jog 0 @14:16:24.74 Archive.home.jog 0 @14:16:25.09 Carousel.home.jog 0 @14:16:25.13 Toroid.home.jog 0 @14:16:25.17 Lysis2.home.jog 0 @14:16:25.22 PinPort.home.jog 0 @14:16:25.25 Plunge.home.jog 0 @14:16:25.31 Lysis1.home.jog 0 @14:16:25.86 Archive.in :homing @14:16:25.93 Air.in :homing @14:16:26.02 Archive.jog 1393 @14:16:26.08 Air.jog 1388 @14:16:26.31 Toroid.in :homing @14:16:26.34 Lysis2.in :homing @14:16:26.37 Lysis1.in :homing @14:16:26.41 PinPort.in :homing @14:16:26.45 Plunge.in :homing @14:16:26.61 Toroid.jog 623 @14:16:26.74 PinPort.jog -106 @14:16:26.77 Plunge.jog 1561 @14:16:26.80 Lysis1.jog 161 @14:16:26.84 Lysis2.jog 1406 @14:16:27.08 PinPort.setRawPosition! 0 @14:16:27.13 PinPort.seek :home @14:16:27.22 PinPort.in :default @14:16:27.31 Air.setRawPosition! 50 @14:16:27.33 Archive.setRawPosition! 50 @14:16:27.41 Air.seek :home @14:16:27.49 Archive.seek :home @14:16:27.58 Lysis1.setRawPosition! 50 @14:16:27.63 Lysis1.seek :home @14:16:27.72 Air.in :default @14:16:27.80 Archive.in :default @14:16:28.26 Lysis2.setRawPosition! 50 @14:16:28.29 Lysis1.in :default @14:16:28.31 Plunge.setRawPosition! 50 @14:16:28.44 Lysis2.seek :home @14:16:28.47 Plunge.seek :home @14:16:28.77 Lysis2.in :default @14:16:28.86 Toroid.setRawPosition! 0 @14:16:28.89 Plunge.in :default @14:16:28.97 Toroid.seek :home @14:16:29.08 Toroid.in :default @14:16:29.32 Toroid.seek :clear @14:16:30.94 Carousel.in :default @14:16:31.06 Carousel.home.seek 27 @14:16:31.31 Selecting Cartridge 14 @14:16:31.62 Carousel.seek 14 @14:16:34.55 Lysis1.in :fast @14:16:34.62 Lysis1.seek :locked @14:16:37.23 Plunge.seek :locked @14:16:37.36 Air.in :fast @14:16:37.43 Air.seek :locked @14:16:39.01 Carousel.reconfigure :locked @14:16:39.15 Powering cartridge bus @14:16:41.77 Gate.setIR 1750,5000,0,6 @14:16:41.89 Gate.setIR 0,5000,0,6 @14:16:42.00 Gate.power :cartridge,:OFF @14:16:42.54 Mounted archive Cartridge @14:16:57.15 -> Cmd.startFiltering @14:16:57.39 Preparing to filter cartridge 14 @14:16:57.72 Priming sample loop w/100ml, bypass w/10ml @14:16:57.74 SamplePump.setPosition! 0ml @14:16:57.79 Bypass.open @14:16:59.30 Intake.open @14:17:00.03 Sea 10.0m deep (29.5psia) @14:17:00.06 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:17:02.64 Vacuum tare is 60% of full scale @14:17:02.82 Pressure.in :default @14:17:02.88 Pressure.hold @14:17:03.07 SamplePump.in :prime @14:17:03.14 SamplePump.seek 10ml,59 seconds @14:17:11.90 Pressure.stop @14:17:11.96 SamplePump.setPosition! 0ml @14:17:12.01 Exhaust.open @14:17:13.47 Bypass.close @14:17:15.01 Pressure.in :default @14:17:15.07 Pressure.hold @14:17:15.56 Sea 10.6m deep (30.4psia) @14:17:15.71 SamplePump.in :prime @14:17:15.79 SamplePump.seek 100ml,5:16 @14:18:52.71 Pressure.stop @14:18:52.78 Bypass.open @14:18:53.52 Intake.close @14:18:53.86 Toroid.in :hold @14:18:53.94 Toroid.seek :crack @14:18:55.47 Toroid.seek :cartridge @14:18:56.58 Toroid.in :default @14:18:56.64 Toroid.seek :clear @14:18:58.60 Pressurizing cartridge to 26psi above 30.1psia @14:18:58.62 SamplePump.setPosition! 0ml @14:18:58.67 Intake.open @14:18:59.32 Bypass.close @14:19:00.04 Exhaust.close @14:19:00.87 Pressure.in :default @14:19:00.94 Pressure.hold @14:19:01.14 SamplePump.in :charge @14:19:01.21 SamplePump.seek 2.3ml,42 seconds @14:19:05.52 Pressure.stop @14:19:05.58 Intake.close @14:19:06.30 Pressurized cartridge with 0.086ml to 27.1psi @14:19:06.31 Isolated @54.4psia (24.2psi) @14:19:06.33 Verifying cartridge holds at least 0.65..0.85 of 54.4psia @14:19:06.37 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:19:10.97 Cartridge pressure fell 1% to 53.7psia @14:19:11.06 Sampling 1000.0ml in 1:25:20 @14:19:11.07 SamplePump.setPosition! 0ml @14:19:11.14 Exhaust.open @14:19:11.92 Intake.open @14:19:12.72 Pressure.in :default @14:19:12.79 Pressure.hold @14:19:12.91 Sea 10.2m deep (29.8psia) @14:19:12.92 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @14:19:13.07 SamplePump.in :sample @14:19:13.15 SamplePump.seek 1000ml,1:23:51 @14:19:15.76 Cartridge 14 filtering 1/1000ml at 0.43ml/s with 23.0psi vacuum 11.6m deep (31.8psia) @14:20:15.79 Cartridge 14 filtering 26/1000ml at 0.42ml/s with 25.7psi vacuum 10.5m deep (30.2psia) @14:21:15.77 Cartridge 14 filtering 50/1000ml at 0.39ml/s with 23.3psi vacuum 8.1m deep (26.7psia) @14:22:15.78 Cartridge 14 filtering 74/1000ml at 0.41ml/s with 22.4psi vacuum 9.4m deep (28.5psia) @14:23:15.76 Cartridge 14 filtering 98/1000ml at 0.38ml/s with 25.7psi vacuum 8.5m deep (27.3psia) @14:24:15.76 Cartridge 14 filtering 121/1000ml at 0.38ml/s with 24.5psi vacuum 10.9m deep (30.8psia) @14:25:15.77 Cartridge 14 filtering 144/1000ml at 0.36ml/s with 23.8psi vacuum 7.9m deep (26.4psia) @14:26:15.76 Cartridge 14 filtering 167/1000ml at 0.37ml/s with 24.2psi vacuum 9.5m deep (28.7psia) @14:27:15.77 Cartridge 14 filtering 189/1000ml at 0.37ml/s with 23.4psi vacuum 10.3m deep (29.9psia) @14:28:15.76 Cartridge 14 filtering 211/1000ml at 0.38ml/s with 23.9psi vacuum 8.4m deep (27.1psia) @14:29:15.76 Cartridge 14 filtering 233/1000ml at 0.38ml/s with 25.3psi vacuum 10.2m deep (29.8psia) @14:30:15.76 Cartridge 14 filtering 254/1000ml at 0.36ml/s with 24.2psi vacuum 10.3m deep (29.9psia) @14:31:15.77 Cartridge 14 filtering 275/1000ml at 0.35ml/s with 20.0psi vacuum 10.6m deep (30.4psia) @14:32:15.76 Cartridge 14 filtering 296/1000ml at 0.33ml/s with 24.2psi vacuum 7.5m deep (25.7psia) @14:33:15.77 Cartridge 14 filtering 317/1000ml at 0.33ml/s with 23.0psi vacuum 8.9m deep (27.9psia) @14:34:15.76 Cartridge 14 filtering 337/1000ml at 0.33ml/s with 21.6psi vacuum 9.3m deep (28.4psia) @14:35:15.77 Cartridge 14 filtering 358/1000ml at 0.34ml/s with 27.6psi vacuum 7.2m deep (25.3psia) @14:36:15.76 Cartridge 14 filtering 378/1000ml at 0.35ml/s with 23.9psi vacuum 9.3m deep (28.4psia) @14:37:15.77 Cartridge 14 filtering 397/1000ml at 0.31ml/s with 24.8psi vacuum 7.7m deep (26.1psia) @14:38:15.76 Cartridge 14 filtering 417/1000ml at 0.30ml/s with 26.0psi vacuum 6.8m deep (24.7psia) @14:39:15.77 Cartridge 14 filtering 436/1000ml at 0.31ml/s with 26.3psi vacuum 8.1m deep (26.6psia) @14:40:15.76 Cartridge 14 filtering 454/1000ml at 0.31ml/s with 25.0psi vacuum 9.2m deep (28.2psia) @14:41:15.77 Cartridge 14 filtering 473/1000ml at 0.29ml/s with 22.8psi vacuum 10.4m deep (30.0psia) @14:42:15.76 Cartridge 14 filtering 491/1000ml at 0.30ml/s with 23.3psi vacuum 6.5m deep (24.3psia) @14:43:15.77 Cartridge 14 filtering 509/1000ml at 0.29ml/s with 23.5psi vacuum 9.2m deep (28.3psia) @14:44:15.77 Cartridge 14 filtering 527/1000ml at 0.29ml/s with 24.8psi vacuum 6.6m deep (24.5psia) @14:45:15.77 Cartridge 14 filtering 545/1000ml at 0.31ml/s with 23.6psi vacuum 9.7m deep (29.0psia) @14:46:15.76 Cartridge 14 filtering 562/1000ml at 0.28ml/s with 24.4psi vacuum 7.4m deep (25.7psia) @14:47:15.77 Cartridge 14 filtering 579/1000ml at 0.28ml/s with 24.9psi vacuum 7.2m deep (25.3psia) @14:48:15.76 Cartridge 14 filtering 596/1000ml at 0.28ml/s with 27.2psi vacuum 5.9m deep (23.4psia) @14:49:15.76 Cartridge 14 filtering 613/1000ml at 0.27ml/s with 25.0psi vacuum 6.8m deep (24.8psia) @14:50:15.76 Cartridge 14 filtering 629/1000ml at 0.27ml/s with 25.0psi vacuum 7.9m deep (26.4psia) @14:51:15.77 Cartridge 14 filtering 646/1000ml at 0.27ml/s with 20.4psi vacuum 12.1m deep (32.6psia) @14:52:15.76 Cartridge 14 filtering 662/1000ml at 0.29ml/s with 24.6psi vacuum 10.1m deep (29.6psia) @14:53:15.77 Cartridge 14 filtering 678/1000ml at 0.25ml/s with 24.9psi vacuum 7.8m deep (26.2psia) @14:54:15.76 Cartridge 14 filtering 693/1000ml at 0.28ml/s with 23.7psi vacuum 8.8m deep (27.6psia) @14:55:15.77 Cartridge 14 filtering 709/1000ml at 0.27ml/s with 24.5psi vacuum 8.3m deep (27.0psia) @14:56:15.76 Cartridge 14 filtering 724/1000ml at 0.25ml/s with 25.0psi vacuum 8.2m deep (26.9psia) @14:57:15.77 Cartridge 14 filtering 740/1000ml at 0.25ml/s with 24.2psi vacuum 6.9m deep (24.9psia) @14:58:15.76 Cartridge 14 filtering 755/1000ml at 0.27ml/s with 20.6psi vacuum 11.3m deep (31.4psia) @14:59:15.77 Cartridge 14 filtering 770/1000ml at 0.25ml/s with 23.7psi vacuum 9.2m deep (28.4psia) @15:00:16.11 Cartridge 14 filtering 785/1000ml at 0.26ml/s with 23.1psi vacuum 10.0m deep (29.4psia) @15:01:15.77 Cartridge 14 filtering 799/1000ml at 0.24ml/s with 25.6psi vacuum 7.3m deep (25.5psia) @15:02:15.77 Cartridge 14 filtering 814/1000ml at 0.26ml/s with 24.9psi vacuum 8.8m deep (27.7psia) @15:03:15.77 Cartridge 14 filtering 828/1000ml at 0.23ml/s with 24.9psi vacuum 8.9m deep (27.8psia) @15:04:15.76 Cartridge 14 filtering 842/1000ml at 0.23ml/s with 22.9psi vacuum 9.7m deep (29.0psia) @15:05:15.77 Cartridge 14 filtering 856/1000ml at 0.23ml/s with 23.9psi vacuum 9.2m deep (28.3psia) @15:06:15.77 Cartridge 14 filtering 870/1000ml at 0.21ml/s with 25.6psi vacuum 6.2m deep (23.9psia) @15:07:15.77 Cartridge 14 filtering 884/1000ml at 0.24ml/s with 24.9psi vacuum 7.5m deep (25.7psia) @15:08:15.76 Cartridge 14 filtering 898/1000ml at 0.24ml/s with 26.7psi vacuum 9.3m deep (28.4psia) @15:09:15.77 Cartridge 14 filtering 911/1000ml at 0.22ml/s with 24.3psi vacuum 7.3m deep (25.6psia) @15:10:15.76 Cartridge 14 filtering 924/1000ml at 0.23ml/s with 28.0psi vacuum 7.7m deep (26.1psia) @15:11:15.76 Cartridge 14 filtering 938/1000ml at 0.23ml/s with 24.6psi vacuum 7.6m deep (25.9psia) @15:12:15.76 Cartridge 14 filtering 951/1000ml at 0.21ml/s with 21.6psi vacuum 9.1m deep (28.2psia) @15:13:15.77 Cartridge 14 filtering 964/1000ml at 0.21ml/s with 24.6psi vacuum 8.3m deep (26.9psia) @15:14:15.76 Cartridge 14 filtering 977/1000ml at 0.21ml/s with 24.8psi vacuum 8.6m deep (27.3psia) @15:15:15.77 Cartridge 14 filtering 990/1000ml at 0.20ml/s with 25.1psi vacuum 7.8m deep (26.2psia) @15:16:04.71 Pressure.stop @15:16:04.84 Intake.close @15:16:04.87 Exhaust.close @15:16:05.07 Toroid.in :hold @15:16:05.15 Toroid.seek :bypass @15:16:06.78 Toroid.in :default @15:16:06.85 Toroid.seek :clear @15:16:08.46 Sampled 1000.0ml @15:16:10.06 -> Cmd.startProcessing @15:16:10.48 Plunge.seek :reagent @15:16:11.28 Sample loop closed. Ready to Deliver Reagents @15:16:11.30 Archive.seek :sealed @15:16:15.25 Archive.seek :evac @15:16:29.30 delay 1:00 @15:17:29.33 Archive.seek :clear @15:17:46.83 RNAlater soak for 10:00 @15:27:46.86 Archive.seek :tVent @15:27:49.31 delay 1 second @15:27:50.33 Archive.seek :evac @15:28:05.87 delay 10 seconds @15:28:15.89 Sample preserved and evacuated @15:28:16.87 -> Cmd.stop @15:28:17.01 Carousel.in :default @15:28:17.13 Carousel.home.seek 14 @15:28:17.47 Plunge.seek :locked @15:28:18.34 Archive.in :unlocking @15:28:18.47 Archive.seek :clear @15:28:18.91 Air.in :unlocking @15:28:19.07 Air.seek :clear @15:28:19.11 Lysis1.in :unlocking @15:28:19.29 Lysis1.seek :clear @15:28:23.41 Gate.power :motor,:OFF @15:28:23.43 Gate.power :core,:OFF @15:28:23.45 Gate.power :analytic1,:OFF @15:28:23.47 Gate.power :analytic2,:OFF @15:28:23.50 Gate.power :analytic3,:OFF @15:28:23.53 Gate.power :main,:OFF @15:28:23.54 Safely stopped and ready to power off ##-- file closed 2023-04-20T22:40:56.365Z ##-- file opened 2023-04-20T22:40:56.372Z @15:40:56.71 -> showStatus @15:40:58.73 -> Cmd.loadCartridge -10 @15:40:58.80 Gate.power :main,:ON,limit to 5000mA @15:40:58.82 Gate.power :analytic1,:ON,limit to 5000mA @15:40:58.84 Gate.power :analytic2,:ON,limit to 5000mA @15:40:58.87 Gate.power :analytic3,:ON,limit to 5000mA @15:40:59.01 Powering core bus @15:41:01.27 SPR Module 1, FW Rev. B, FPGA Rev. C @15:41:01.71 Archive.configure :default @15:41:01.81 Air.configure :default @15:41:01.88 Lysis1.configure :default @15:41:01.94 Lysis2.configure :default @15:41:02.03 Plunge.configure :default @15:41:02.12 SamplePump.configure :default @15:41:02.21 Toroid.configure :default @15:41:02.30 Carousel.configure :default @15:41:02.39 Pressure.configure :default @15:41:02.48 ExtraPump.configure :default @15:41:02.55 PinPort.configure :default @15:41:02.67 All elf controllers running firmware version 4.83 @15:41:02.70 Gate.power :motor,:ON,limit to 5000mA @15:41:02.94 Archive.home.jog 0 @15:41:03.39 Carousel.home.jog 0 @15:41:03.44 Toroid.home.jog 0 @15:41:03.46 Lysis1.home.jog 0 @15:41:03.50 Lysis2.home.jog 0 @15:41:03.56 PinPort.home.jog 0 @15:41:03.58 Air.home.jog 0 @15:41:03.63 Plunge.home.jog 0 @15:41:04.19 Archive.in :homing @15:41:04.41 Plunge.in :homing @15:41:04.44 Archive.jog 1389 @15:41:04.47 PinPort.in :homing @15:41:04.50 Lysis2.in :homing @15:41:04.54 Air.in :homing @15:41:04.58 Toroid.in :homing @15:41:04.62 Lysis1.in :homing @15:41:04.86 Plunge.jog 1550 @15:41:04.89 PinPort.jog -104 @15:41:04.93 Lysis2.jog 1404 @15:41:04.96 Toroid.jog 615 @15:41:05.01 Lysis1.jog 158 @15:41:05.06 Air.jog 1386 @15:41:05.34 PinPort.setRawPosition! 0 @15:41:05.38 PinPort.seek :home @15:41:05.47 PinPort.in :default @15:41:05.60 Lysis1.setRawPosition! 50 @15:41:05.67 Lysis1.seek :home @15:41:05.86 Archive.setRawPosition! 50 @15:41:05.91 Archive.seek :home @15:41:05.98 Lysis1.in :default @15:41:06.23 Plunge.setRawPosition! 50 @15:41:06.25 Archive.in :default @15:41:06.35 Plunge.seek :home @15:41:06.81 Air.setRawPosition! 50 @15:41:06.83 Lysis2.setRawPosition! 50 @15:41:06.90 Air.seek :home @15:41:06.95 Lysis2.seek :home @15:41:07.09 Plunge.in :default @15:41:07.21 Toroid.setRawPosition! 0 @15:41:07.28 Toroid.seek :home @15:41:07.37 Lysis2.in :default @15:41:07.41 Air.in :default @15:41:07.44 Toroid.in :default @15:41:07.84 Toroid.seek :clear @15:41:09.47 Carousel.in :default @15:41:09.59 Carousel.home.seek 14 @15:41:09.84 Selecting Cartridge 25 @15:41:10.11 Carousel.seek 25 @15:41:12.63 Lysis1.in :fast @15:41:12.69 Lysis1.seek :locked @15:41:15.29 Plunge.seek :locked @15:41:15.43 Air.in :fast @15:41:15.50 Air.seek :locked @15:41:17.07 Carousel.reconfigure :locked @15:41:17.22 Powering cartridge bus @15:41:19.85 Gate.setIR 1750,5000,0,6 @15:41:19.97 Gate.setIR 0,5000,0,6 @15:41:20.05 PuckHeater.configure :default @15:41:20.11 Gate.power :heat,:ON,limit to 5000mA @15:41:20.81 Mounted lysis Cartridge board#Q05061 @15:41:20.86 Using this cartridge's heat loss curve for HeatingAccelInCarousel @15:41:36.30 -> Cmd.startFiltering @15:41:36.55 Preparing to filter cartridge 25 @15:41:36.83 Priming sample loop w/100ml, bypass w/10ml @15:41:36.85 SamplePump.setPosition! 0ml @15:41:36.90 Bypass.open @15:41:38.38 Intake.open @15:41:39.19 Sea 10.1m deep (29.6psia) @15:41:39.20 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:41:41.80 Vacuum tare is 60% of full scale @15:41:41.93 Pressure.in :default @15:41:42.00 Pressure.hold @15:41:42.18 SamplePump.in :prime @15:41:42.26 SamplePump.seek 10ml,59 seconds @15:41:51.02 Pressure.stop @15:41:51.07 SamplePump.setPosition! 0ml @15:41:51.13 Exhaust.open @15:41:52.63 Bypass.close @15:41:54.12 Pressure.in :default @15:41:54.19 Pressure.hold @15:41:54.31 Sea 9.9m deep (29.3psia) @15:41:54.45 SamplePump.in :prime @15:41:54.53 SamplePump.seek 100ml,5:16 @15:43:31.23 Pressure.stop @15:43:31.31 Bypass.open @15:43:32.01 Intake.close @15:43:32.77 Toroid.in :hold @15:43:32.85 Toroid.seek :crack @15:43:34.38 Toroid.seek :cartridge @15:43:35.48 Toroid.in :default @15:43:35.54 Toroid.seek :clear @15:43:37.51 Pressurizing cartridge to 26psi above 29.5psia @15:43:37.53 SamplePump.setPosition! 0ml @15:43:37.59 Intake.open @15:43:38.23 Bypass.close @15:43:38.94 Exhaust.close @15:43:39.78 Pressure.in :default @15:43:39.85 Pressure.hold @15:43:40.04 SamplePump.in :charge @15:43:40.11 SamplePump.seek 2.3ml,42 seconds @15:43:48.82 Pressure.stop @15:43:48.88 Intake.close @15:43:49.26 Pressurized cartridge with 0.358ml to 26.1psi @15:43:49.27 Isolated @53.7psia (24.3psi) @15:43:49.30 Verifying cartridge holds at least 0.65..0.85 of 53.7psia @15:43:49.38 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:43:54.03 Sampling 1000.0ml in 1:25:20 @15:43:54.05 SamplePump.setPosition! 0ml @15:43:54.11 Exhaust.open @15:43:54.85 Intake.open @15:43:55.68 Pressure.in :default @15:43:55.75 Pressure.hold @15:43:55.87 Sea 10.6m deep (30.4psia) @15:43:55.88 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @15:43:56.04 SamplePump.in :sample @15:43:56.11 SamplePump.seek 1000ml,1:23:51 @15:43:58.72 Cartridge 25 filtering 1/1000ml at 0.44ml/s with 23.6psi vacuum 11.8m deep (32.1psia) @15:44:58.72 Cartridge 25 filtering 25/1000ml at 0.42ml/s with 25.4psi vacuum 9.6m deep (28.9psia) @15:45:58.72 Cartridge 25 filtering 48/1000ml at 0.40ml/s with 23.9psi vacuum 10.8m deep (30.7psia) @15:46:58.72 Cartridge 25 filtering 72/1000ml at 0.36ml/s with 22.5psi vacuum 8.7m deep (27.5psia) @15:47:58.73 Cartridge 25 filtering 94/1000ml at 0.37ml/s with 21.0psi vacuum 10.0m deep (29.4psia) @15:48:58.72 Cartridge 25 filtering 117/1000ml at 0.38ml/s with 24.5psi vacuum 9.1m deep (28.2psia) @15:49:58.72 Cartridge 25 filtering 139/1000ml at 0.34ml/s with 23.4psi vacuum 8.4m deep (27.0psia) @15:50:58.72 Cartridge 25 filtering 160/1000ml at 0.35ml/s with 21.0psi vacuum 9.5m deep (28.7psia) @15:51:58.72 Cartridge 25 filtering 181/1000ml at 0.35ml/s with 24.6psi vacuum 9.4m deep (28.5psia) @15:52:58.73 Cartridge 25 filtering 202/1000ml at 0.33ml/s with 22.7psi vacuum 9.7m deep (29.0psia) @15:53:58.72 Cartridge 25 filtering 223/1000ml at 0.33ml/s with 25.8psi vacuum 7.7m deep (26.1psia) @15:54:58.72 Cartridge 25 filtering 243/1000ml at 0.35ml/s with 24.7psi vacuum 10.2m deep (29.7psia) @15:55:58.72 Cartridge 25 filtering 263/1000ml at 0.34ml/s with 24.7psi vacuum 7.6m deep (26.0psia) @15:56:58.72 Cartridge 25 filtering 283/1000ml at 0.33ml/s with 25.7psi vacuum 8.6m deep (27.4psia) @15:57:58.72 Cartridge 25 filtering 302/1000ml at 0.30ml/s with 23.4psi vacuum 10.1m deep (29.6psia) @15:58:58.72 Cartridge 25 filtering 322/1000ml at 0.33ml/s with 22.9psi vacuum 8.4m deep (27.0psia) @15:59:58.73 Cartridge 25 filtering 340/1000ml at 0.29ml/s with 24.0psi vacuum 8.3m deep (27.0psia) @16:00:58.73 Cartridge 25 filtering 359/1000ml at 0.31ml/s with 26.3psi vacuum 8.5m deep (27.3psia) @16:01:58.73 Cartridge 25 filtering 378/1000ml at 0.31ml/s with 26.6psi vacuum 8.1m deep (26.6psia) @16:02:58.73 Cartridge 25 filtering 396/1000ml at 0.28ml/s with 26.4psi vacuum 6.8m deep (24.8psia) @16:03:58.72 Cartridge 25 filtering 414/1000ml at 0.31ml/s with 23.1psi vacuum 9.0m deep (27.9psia) @16:04:58.73 Cartridge 25 filtering 431/1000ml at 0.29ml/s with 22.4psi vacuum 8.8m deep (27.8psia) @16:05:58.73 Cartridge 25 filtering 449/1000ml at 0.29ml/s with 23.6psi vacuum 8.2m deep (26.8psia) @16:06:58.73 Cartridge 25 filtering 466/1000ml at 0.30ml/s with 23.4psi vacuum 10.6m deep (30.3psia) @16:07:58.72 Cartridge 25 filtering 483/1000ml at 0.28ml/s with 22.5psi vacuum 7.9m deep (26.4psia) @16:08:58.73 Cartridge 25 filtering 500/1000ml at 0.28ml/s with 21.1psi vacuum 9.3m deep (28.5psia) @16:09:58.72 Cartridge 25 filtering 517/1000ml at 0.27ml/s with 25.9psi vacuum 8.7m deep (27.5psia) @16:10:58.73 Cartridge 25 filtering 533/1000ml at 0.27ml/s with 24.6psi vacuum 8.6m deep (27.4psia) @16:11:58.72 Cartridge 25 filtering 550/1000ml at 0.26ml/s with 24.0psi vacuum 8.1m deep (26.7psia) @16:12:58.73 Cartridge 25 filtering 566/1000ml at 0.26ml/s with 26.4psi vacuum 8.5m deep (27.2psia) @16:13:58.72 Cartridge 25 filtering 582/1000ml at 0.25ml/s with 25.4psi vacuum 8.4m deep (27.1psia) @16:14:58.72 Cartridge 25 filtering 597/1000ml at 0.28ml/s with 25.2psi vacuum 8.7m deep (27.5psia) @16:15:58.74 Cartridge 25 filtering 613/1000ml at 0.26ml/s with 25.4psi vacuum 8.2m deep (26.8psia) @16:16:58.72 Cartridge 25 filtering 628/1000ml at 0.26ml/s with 24.3psi vacuum 7.8m deep (26.2psia) @16:17:58.72 Cartridge 25 filtering 643/1000ml at 0.25ml/s with 23.6psi vacuum 8.8m deep (27.7psia) @16:18:58.72 Cartridge 25 filtering 659/1000ml at 0.25ml/s with 25.6psi vacuum 8.4m deep (27.1psia) @16:19:58.72 Cartridge 25 filtering 673/1000ml at 0.25ml/s with 25.4psi vacuum 8.5m deep (27.2psia) @16:20:58.72 Cartridge 25 filtering 688/1000ml at 0.23ml/s with 24.5psi vacuum 8.1m deep (26.7psia) @16:21:58.72 Cartridge 25 filtering 703/1000ml at 0.23ml/s with 24.0psi vacuum 8.5m deep (27.3psia) @16:22:59.05 Cartridge 25 filtering 717/1000ml at 0.26ml/s with 23.6psi vacuum 10.0m deep (29.4psia) @16:23:58.73 Cartridge 25 filtering 731/1000ml at 0.25ml/s with 23.6psi vacuum 9.3m deep (28.4psia) @16:24:58.72 Cartridge 25 filtering 745/1000ml at 0.22ml/s with 23.7psi vacuum 8.9m deep (27.9psia) @16:25:58.73 Cartridge 25 filtering 759/1000ml at 0.24ml/s with 22.8psi vacuum 8.1m deep (26.7psia) @16:26:58.72 Cartridge 25 filtering 773/1000ml at 0.22ml/s with 23.4psi vacuum 9.3m deep (28.5psia) @16:27:58.73 Cartridge 25 filtering 787/1000ml at 0.25ml/s with 23.8psi vacuum 8.8m deep (27.7psia) @16:28:58.72 Cartridge 25 filtering 800/1000ml at 0.24ml/s with 22.6psi vacuum 8.7m deep (27.5psia) @16:29:58.72 Cartridge 25 filtering 814/1000ml at 0.22ml/s with 24.4psi vacuum 8.0m deep (26.5psia) @16:30:58.72 Cartridge 25 filtering 827/1000ml at 0.21ml/s with 24.5psi vacuum 8.5m deep (27.2psia) @16:31:58.72 Cartridge 25 filtering 840/1000ml at 0.24ml/s with 24.4psi vacuum 9.3m deep (28.4psia) @16:32:58.72 Cartridge 25 filtering 853/1000ml at 0.21ml/s with 25.3psi vacuum 8.8m deep (27.7psia) @16:33:58.72 Cartridge 25 filtering 866/1000ml at 0.21ml/s with 24.8psi vacuum 9.1m deep (28.2psia) @16:34:58.72 Cartridge 25 filtering 879/1000ml at 0.21ml/s with 26.6psi vacuum 8.5m deep (27.3psia) @16:35:58.72 Cartridge 25 filtering 892/1000ml at 0.20ml/s with 25.0psi vacuum 8.1m deep (26.7psia) @16:36:58.72 Cartridge 25 filtering 905/1000ml at 0.20ml/s with 24.7psi vacuum 9.0m deep (28.1psia) @16:37:58.72 Cartridge 25 filtering 917/1000ml at 0.22ml/s with 23.8psi vacuum 8.6m deep (27.4psia) @16:38:58.72 Cartridge 25 filtering 929/1000ml at 0.20ml/s with 25.6psi vacuum 9.3m deep (28.4psia) @16:39:58.72 Cartridge 25 filtering 942/1000ml at 0.20ml/s with 26.8psi vacuum 7.5m deep (25.8psia) @16:40:58.72 Cartridge 25 filtering 954/1000ml at 0.20ml/s with 25.3psi vacuum 9.0m deep (28.0psia) @16:41:58.72 Cartridge 25 filtering 966/1000ml at 0.21ml/s with 24.2psi vacuum 7.9m deep (26.4psia) @16:42:58.72 Cartridge 25 filtering 978/1000ml at 0.20ml/s with 25.2psi vacuum 9.0m deep (28.1psia) @16:43:58.72 Cartridge 25 filtering 991/1000ml at 0.20ml/s with 25.7psi vacuum 7.9m deep (26.4psia) @16:44:44.74 Pressure.stop @16:44:44.86 Intake.close @16:44:44.90 Exhaust.close @16:44:45.08 Toroid.in :hold @16:44:45.16 Toroid.seek :bypass @16:44:46.79 Toroid.in :default @16:44:46.86 Toroid.seek :clear @16:44:48.47 Sampled 1000.0ml @16:44:49.95 -> Cmd.startProcessing @16:44:51.40 SPR.command \"read_sequences\" @16:44:51.76 SPR.command \"stats_on\" @16:44:51.89 SPR.command \"start 0 1\" @16:44:52.62 SPR.start /var/log/esp/spr23apr2016h.spr.bz2 @16:44:54.61 Handoff.select :waste @16:45:01.31 Syringe tare is 17% of full scale @16:45:01.34 PinPort.in :locking @16:45:01.42 PinPort.seek :locked @16:45:01.89 Archive.in :fast @16:45:01.95 Lysis2.in :fast @16:45:02.02 Lysis1.seek 1350uL @16:45:02.12 Archive.seek :full @16:45:02.20 Lysis2.seek :full @16:45:04.16 Plunge.seek :reagent @16:45:05.04 Evacuating puck with filter bubble point of 50psi @16:45:05.06 PinPort.seek :locked @16:45:05.15 Handoff.select :waste @16:45:11.73 Air syringe pumping up to 1 strokes to reach 8psi @16:45:11.74 Air stroke #1 of 1 @16:45:11.84 Air.in :pressurize @16:45:11.91 Air.seek :pressurized @16:45:17.65 0.4psi after pressurize @16:45:17.66 Air.in :compress @16:45:17.75 Air.seek :evac @16:45:20.78 0.6psi after compress @16:45:31.35 0.0psi after compress (stable) @16:45:31.37 Air.in :default @16:45:31.44 Air.hold @16:45:31.67 at -0.0psi Opened to waste @16:45:31.68 Handoff.select :blocked @16:45:31.83 Archive syringe pumping up to 8 strokes to reach 50psi @16:45:31.84 Archive stroke #1 of 8 @16:45:31.94 Archive.in :pressurize @16:45:32.05 Archive.seek :pressurized @16:45:44.56 7.6psi after pressurize @16:46:17.15 20.0psi after pressurize (stable) @16:46:17.16 Archive.in :compress @16:46:17.24 Archive.seek :empty @16:46:31.99 28.7psi after compress @16:47:02.08 35.3psi after compress (stable) @16:47:02.10 Archive.in :gulp @16:47:02.18 Archive.seek :topVent @16:47:08.44 35.9psi after gulp @16:47:14.03 35.8psi after gulp (stable) @16:47:14.04 Archive stroke #2 of 8 @16:47:14.17 Archive.in :pressurize @16:47:14.24 Archive.seek :pressurized @16:47:26.74 35.0psi after pressurize @16:47:32.32 35.5psi after pressurize (stable) @16:47:32.34 Archive.in :compress @16:47:32.42 Archive.seek :empty @16:47:46.60 Reached 50.3 of 50.0psi after pumping 3450uL (1.99 strokes) @16:47:46.61 Archive.in :default @16:47:46.69 Archive.hold @16:47:46.76 Handoff.select :waste @16:47:54.96 delay 1:00 @16:48:54.98 Handoff.select :blocked @16:49:22.67 Handoff.select :waste @16:49:22.81 4.3psi at evacEnd @16:49:22.82 Handoff.select :blocked @16:49:22.96 Archive.in :fast @16:49:23.02 Archive.seek :topVent @16:49:37.66 Stable @2.3psi after Handoff blocked @16:49:37.68 Archive syringe pumping up to 8 strokes to reach 32psi @16:49:37.69 Archive stroke #1 of 8 @16:49:37.80 Archive.in :pressurize @16:49:37.88 Archive.seek :pressurized @16:49:50.46 3.4psi after pressurize @16:49:50.48 Archive.in :compress @16:49:50.56 Archive.seek :empty @16:50:05.19 7.6psi after compress @16:50:38.77 17.3psi after compress (stable) @16:50:38.78 Archive.in :gulp @16:50:38.87 Archive.seek :topVent @16:50:45.13 18.3psi after gulp @16:50:50.73 18.8psi after gulp (stable) @16:50:50.77 Archive stroke #2 of 8 @16:50:50.87 Archive.in :pressurize @16:50:50.95 Archive.seek :pressurized @16:51:03.45 19.4psi after pressurize @16:51:03.46 Archive.in :compress @16:51:03.55 Archive.seek :empty @16:51:18.22 22.0psi after compress @16:51:47.40 Reached 32.3 of 32.3psi after pumping 3460uL (2.00 strokes) @16:51:47.41 Archive.in :default @16:51:47.52 Archive.hold @16:51:47.59 Handoff.select :waste @16:51:58.28 delay 1:00 @16:52:58.30 Handoff.select :blocked @16:53:21.02 Handoff.select :waste @16:53:21.16 2.0psi at evacEnd @16:53:21.18 Air.seek :fullVent @16:53:22.61 Archive.in :fast @16:53:22.68 Archive.seek :topVent @16:53:29.79 Seawater evacuated, Pressure above/below filter released. @16:53:29.88 Lysis1.in :slow @16:53:29.95 Lysis1.seek 1050uL @16:54:00.00 delay 5 seconds @16:54:05.02 Handoff.select :blocked @16:54:05.21 Lysis1.seek 450uL @16:55:05.52 delay 3 seconds @16:55:08.59 Air.in mix with 1.40048 parts Lysis1 @16:55:09.08 Air.seek :evac @16:55:09.12 Lysis1.seek 300uL @16:55:24.25 Lysis1.seek :empty @16:55:54.40 Lysis buffer delivered @19.9psi @16:55:54.42 Polling Can every 2:00 @16:55:54.97 PuckHeater.configure :default #ambient 12.9C @16:55:55.02 PuckHeater.seek 81.2C @16:57:08.28 PuckHeater.hold 2:00,81.2C @16:59:08.30 Polling Can every 2:00 @16:59:08.84 PuckHeater.configure :default #ambient 13.0C @16:59:08.90 PuckHeater.hold 30 seconds,80.1C @16:59:39.07 Polling Can every 2:00 @16:59:39.61 PuckHeater.configure :default #ambient 13.0C @16:59:39.67 PuckHeater.hold 30 seconds,79.6C @17:00:09.85 Polling Can every 2:00 @17:00:10.39 PuckHeater.configure :default #ambient 12.9C @17:00:10.45 PuckHeater.hold 10:00,79C @17:10:09.98 Lysis complete. Syringe pressure @26.7psi @17:10:10.01 Archive syringe pumping up to 5 strokes to reach 40psi @17:10:10.02 Archive stroke #1 of 5 @17:10:10.12 Archive.in :pressurize @17:10:10.19 Archive.seek :pressurized @17:10:22.69 26.4psi after pressurize @17:10:28.28 26.5psi after pressurize (stable) @17:10:28.32 Archive.in :compress @17:10:28.40 Archive.seek :empty @17:10:36.87 Reached 40.6 of 40.0psi after pumping 1596uL (0.92 strokes) @17:10:36.89 Archive.in :default @17:10:36.97 Archive.hold @17:10:37.05 delay 2 seconds @17:10:39.07 Archive.in :fast @17:10:39.13 Archive.seek :full @17:10:45.84 Lysis1.seek 138uL @17:10:59.72 delay 1 second @17:11:00.75 Archive.seek :full @17:11:00.78 Lysis1.seek :empty @17:11:14.68 Handoff.select :blocked @17:11:14.77 Initial air pressure @50.1psi @17:11:14.83 Lysis1.in :extract @17:11:14.95 Lysis1.seek 600uL,nil @17:11:17.17 Archive syringe pumping up to 6 strokes to reach 25psi @17:11:17.18 Archive stroke #1 of 6 @17:11:17.31 Archive.in :pressurize @17:11:17.39 Archive.seek :pressurized @17:11:29.94 15.6psi after pressurize @17:11:47.01 15.1psi after pressurize (stable) @17:11:47.03 Archive.in :compress @17:11:47.12 Archive.seek :empty @17:11:56.87 Archive.in :default @17:11:56.94 Archive.hold @17:11:57.08 Initial 600uL lysate @15.9psi @17:11:57.09 Handoff.select :blocked @17:11:57.22 Initial air pressure @16.5psi @17:11:57.69 Lysis1.seek 825uL,nil @17:11:58.38 Archive syringe pumping up to 6 strokes to reach 17psi @17:11:58.40 Archive stroke #1 of 6 @17:11:58.49 Archive.in :compress @17:11:58.59 Archive.seek :empty @17:12:00.22 Reached 17.0 of 17.0psi after pumping 1659uL (0.96 strokes) @17:12:00.24 Archive.in :default @17:12:00.31 Archive.hold @17:12:01.04 Archive syringe pumping up to 6 strokes to reach 17psi @17:12:01.05 Archive stroke #1 of 6 @17:12:01.19 Archive.in :compress @17:12:01.26 Archive.seek :empty @17:12:03.17 Reached 17.0 of 17.0psi after pumping 1697uL (0.98 strokes) @17:12:03.19 Archive.in :default @17:12:03.26 Archive.hold @17:12:03.99 Archive syringe pumping up to 6 strokes to reach 17psi @17:12:04.00 Archive stroke #1 of 6 @17:12:04.09 Archive.in :compress @17:12:04.17 Archive.seek :empty @17:12:05.39 Archive.in :default @17:12:05.45 Archive.hold @17:12:05.68 Recovered 825uL lysate @14.0psi @17:12:05.71 Air.in :halfSpeed @17:12:05.81 Air.seek :fullVent @17:12:08.01 Puck Top Vented, bottom @1.7psi @17:12:08.02 Handoff.select :waste @17:12:08.15 delay 5 seconds @17:12:13.22 Handoff open to waste, bottom @-0.0psi @17:12:13.25 Air.in :default @17:12:13.34 Air.seek :evac @17:12:14.76 Lysis2.in :DAmix @17:12:14.88 Lysis1.in :DAmix @17:12:15.39 Lysis2.seek 1480uL @17:12:15.42 Lysis1.seek 575uL @17:12:50.57 delay 5 seconds @17:12:55.59 Handoff.select SPR @17:12:55.70 SPR.command \"start 2 14\" @17:12:56.31 SPR stabilized @17:12:56.44 delay 10 seconds @17:13:06.46 SPR.command \"start 1 0\" @17:13:09.67 Waiting up to 4:00 for SPR to enter state 1 @17:15:13.92 SPR.command \"hold_on\" @17:15:15.34 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @17:15:15.81 Lysis2.seek 1180uL @17:15:15.89 Lysis1.seek 275uL @17:15:57.90 delay 15 seconds @17:16:12.91 SPR.command \"hold_off\" @17:16:16.00 Waiting up to 38:00 for SPR to enter state 7 @17:34:15.58 SPRsummary:15.2RIU,151RIU,161RIU,221RIU,2.75ng/L,none,3.15ng/L after 3130s @17:40:52.80 SPR analysis completed @17:40:58.41 Ended log spr23apr2016h.spr.bz2 @17:40:58.42 SPR.command \"stats_off\" @17:40:59.77 -> Cmd.stop @17:40:59.88 Handoff.select :close @17:41:00.01 Gate.power :heat,:OFF @17:41:00.03 Gate.power :cartridge,:OFF @17:41:00.12 PinPort.in :unlocking @17:41:00.19 PinPort.seek :clear @17:41:00.66 Carousel.in :default @17:41:00.78 Carousel.home.seek 25 @17:41:01.10 Plunge.seek :locked @17:41:01.97 Archive.in :unlocking @17:41:02.11 Air.in :unlocking @17:41:02.16 Archive.seek :clear @17:41:02.35 Air.seek :clear @17:41:02.46 Lysis1.in :unlocking @17:41:02.60 Lysis2.in :unlocking @17:41:02.64 Lysis1.seek :clear @17:41:02.75 Lysis2.seek :clear @17:41:06.73 Gate.power :motor,:OFF @17:41:06.75 Gate.power :core,:OFF @17:41:06.77 Gate.power :analytic1,:OFF @17:41:06.79 Gate.power :analytic2,:OFF @17:41:06.81 Gate.power :analytic3,:OFF @17:41:06.84 Gate.power :main,:OFF @17:41:06.86 Safely stopped and ready to power off ##-- file closed 2023-04-21T03:02:56.089Z ##-- file opened 2023-04-21T03:02:56.091Z @20:02:56.31 -> showStatus @20:02:58.33 -> Cmd.loadCartridge -10 @20:02:58.39 Gate.power :main,:ON,limit to 5000mA @20:02:58.42 Gate.power :analytic1,:ON,limit to 5000mA @20:02:58.44 Gate.power :analytic2,:ON,limit to 5000mA @20:02:58.47 Gate.power :analytic3,:ON,limit to 5000mA @20:02:58.60 Powering core bus @20:03:00.87 SPR Module 1, FW Rev. B, FPGA Rev. C @20:03:01.31 Archive.configure :default @20:03:01.40 Air.configure :default @20:03:01.47 Lysis1.configure :default @20:03:01.54 Lysis2.configure :default @20:03:01.62 Plunge.configure :default @20:03:01.71 SamplePump.configure :default @20:03:01.81 Toroid.configure :default @20:03:01.90 Carousel.configure :default @20:03:01.99 Pressure.configure :default @20:03:02.08 ExtraPump.configure :default @20:03:02.15 PinPort.configure :default @20:03:02.22 All elf controllers running firmware version 4.83 @20:03:02.25 Gate.power :motor,:ON,limit to 5000mA @20:03:02.38 Archive.home.jog 0 @20:03:02.96 Carousel.home.jog 0 @20:03:03.01 Toroid.home.jog 0 @20:03:03.04 Lysis2.home.jog 0 @20:03:03.09 Lysis1.home.jog 0 @20:03:03.13 PinPort.home.jog 0 @20:03:03.16 Plunge.home.jog 0 @20:03:03.18 Air.home.jog 0 @20:03:03.75 Archive.in :homing @20:03:04.05 Plunge.in :homing @20:03:04.08 Lysis1.in :homing @20:03:04.11 Archive.jog 1388 @20:03:04.15 PinPort.in :homing @20:03:04.19 Lysis2.in :homing @20:03:04.22 Toroid.in :homing @20:03:04.27 Air.in :homing @20:03:04.52 Plunge.jog 1555 @20:03:04.54 Lysis1.jog 155 @20:03:04.58 PinPort.jog -105 @20:03:04.61 Lysis2.jog 1402 @20:03:04.63 Toroid.jog 618 @20:03:04.69 Air.jog 1386 @20:03:04.98 PinPort.setRawPosition! 0 @20:03:05.04 PinPort.seek :home @20:03:05.12 PinPort.in :default @20:03:05.20 Lysis1.setRawPosition! 50 @20:03:05.25 Lysis1.seek :home @20:03:05.52 Lysis1.in :default @20:03:05.60 Archive.setRawPosition! 50 @20:03:05.66 Archive.seek :home @20:03:05.90 Plunge.setRawPosition! 50 @20:03:05.96 Plunge.seek :home @20:03:06.43 Lysis2.setRawPosition! 50 @20:03:06.45 Air.setRawPosition! 50 @20:03:06.47 Archive.in :default @20:03:06.60 Lysis2.seek :home @20:03:06.63 Air.seek :home @20:03:06.76 Plunge.in :default @20:03:06.91 Toroid.setRawPosition! 0 @20:03:06.98 Toroid.seek :home @20:03:07.09 Lysis2.in :default @20:03:07.11 Air.in :default @20:03:07.14 Toroid.in :default @20:03:07.54 Toroid.seek :clear @20:03:09.16 Carousel.in :default @20:03:09.28 Carousel.home.seek 25 @20:03:09.54 Selecting Cartridge 23 @20:03:09.81 Carousel.seek 23 @20:03:10.50 Lysis1.in :fast @20:03:10.57 Lysis1.seek :locked @20:03:13.18 Plunge.seek :locked @20:03:13.32 Air.in :fast @20:03:13.39 Air.seek :locked @20:03:14.96 Carousel.reconfigure :locked @20:03:15.11 Powering cartridge bus @20:03:17.72 Gate.setIR 1750,5000,0,6 @20:03:17.85 Gate.setIR 0,5000,0,6 @20:03:17.93 PuckHeater.configure :default @20:03:17.99 Gate.power :heat,:ON,limit to 5000mA @20:03:18.68 Mounted lysis Cartridge board#Q05042 @20:03:18.71 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:03:35.94 -> Cmd.startFiltering @20:03:36.19 Preparing to filter cartridge 23 @20:03:36.47 Priming sample loop w/100ml, bypass w/10ml @20:03:36.48 SamplePump.setPosition! 0ml @20:03:36.54 Bypass.open @20:03:38.01 Intake.open @20:03:38.75 Sea 13.0m deep (34.0psia) @20:03:38.77 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @20:03:41.35 Vacuum tare is 60% of full scale @20:03:41.49 Pressure.in :default @20:03:41.55 Pressure.hold @20:03:41.74 SamplePump.in :prime @20:03:41.82 SamplePump.seek 10ml,59 seconds @20:03:50.57 Pressure.stop @20:03:50.62 SamplePump.setPosition! 0ml @20:03:50.67 Exhaust.open @20:03:52.51 Bypass.close @20:03:54.04 Pressure.in :default @20:03:54.12 Pressure.hold @20:03:54.24 Sea 13.2m deep (34.2psia) @20:03:54.39 SamplePump.in :prime @20:03:54.46 SamplePump.seek 100ml,5:16 @20:05:31.52 Pressure.stop @20:05:31.59 Bypass.open @20:05:32.28 Intake.close @20:05:33.04 Toroid.in :hold @20:05:33.11 Toroid.seek :crack @20:05:34.64 Toroid.seek :cartridge @20:05:35.74 Toroid.in :default @20:05:35.82 Toroid.seek :clear @20:05:37.78 Pressurizing cartridge to 26psi above 34.5psia @20:05:37.80 SamplePump.setPosition! 0ml @20:05:37.85 Intake.open @20:05:38.53 Bypass.close @20:05:39.23 Exhaust.close @20:05:40.05 Pressure.in :default @20:05:40.12 Pressure.hold @20:05:40.31 SamplePump.in :charge @20:05:40.39 SamplePump.seek 2.3ml,42 seconds @20:05:48.49 Pressure.stop @20:05:48.55 Intake.close @20:05:49.32 Pressurized cartridge with 0.309ml to 26.3psi @20:05:49.33 Isolated @60.3psia (25.8psi) @20:05:49.35 Verifying cartridge holds at least 0.65..0.85 of 60.3psia @20:05:49.39 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @20:05:53.97 Cartridge pressure fell 2% to 59.0psia @20:05:54.06 Sampling 1000.0ml in 1:25:20 @20:05:54.08 SamplePump.setPosition! 0ml @20:05:54.15 Exhaust.open @20:05:54.92 Intake.open @20:05:55.74 Pressure.in :default @20:05:55.81 Pressure.hold @20:05:55.93 Sea 14.1m deep (35.5psia) @20:05:55.94 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @20:05:56.10 SamplePump.in :sample @20:05:56.17 SamplePump.seek 1000ml,1:23:51 @20:05:58.78 Cartridge 23 filtering 1/1000ml at 0.38ml/s with 23.8psi vacuum 11.9m deep (32.3psia) @20:06:58.78 Cartridge 23 filtering 23/1000ml at 0.35ml/s with 23.6psi vacuum 11.8m deep (32.2psia) @20:07:59.16 Cartridge 23 filtering 46/1000ml at 0.38ml/s with 22.4psi vacuum 15.3m deep (37.3psia) @20:08:58.79 Cartridge 23 filtering 67/1000ml at 0.37ml/s with 23.3psi vacuum 14.0m deep (35.4psia) @20:09:58.78 Cartridge 23 filtering 89/1000ml at 0.34ml/s with 22.8psi vacuum 11.5m deep (31.7psia) @20:10:58.79 Cartridge 23 filtering 109/1000ml at 0.33ml/s with 26.8psi vacuum 10.5m deep (30.3psia) @20:11:58.79 Cartridge 23 filtering 130/1000ml at 0.35ml/s with 22.6psi vacuum 13.0m deep (33.9psia) @20:12:58.79 Cartridge 23 filtering 150/1000ml at 0.33ml/s with 25.2psi vacuum 10.9m deep (30.9psia) @20:13:58.78 Cartridge 23 filtering 170/1000ml at 0.31ml/s with 25.2psi vacuum 10.4m deep (30.1psia) @20:14:58.79 Cartridge 23 filtering 190/1000ml at 0.31ml/s with 22.8psi vacuum 13.5m deep (34.7psia) @20:15:58.78 Cartridge 23 filtering 209/1000ml at 0.30ml/s with 23.6psi vacuum 13.1m deep (34.0psia) @20:16:58.79 Cartridge 23 filtering 229/1000ml at 0.29ml/s with 23.8psi vacuum 12.4m deep (33.0psia) @20:17:58.79 Cartridge 23 filtering 247/1000ml at 0.30ml/s with 22.7psi vacuum 12.4m deep (33.0psia) @20:18:58.79 Cartridge 23 filtering 266/1000ml at 0.32ml/s with 25.4psi vacuum 11.2m deep (31.3psia) @20:19:58.83 Cartridge 23 filtering 284/1000ml at 0.30ml/s with 23.3psi vacuum 12.5m deep (33.2psia) @20:20:58.79 Cartridge 23 filtering 302/1000ml at 0.29ml/s with 21.7psi vacuum 12.7m deep (33.5psia) @20:21:58.79 Cartridge 23 filtering 320/1000ml at 0.31ml/s with 24.2psi vacuum 13.4m deep (34.6psia) @20:22:58.78 Cartridge 23 filtering 338/1000ml at 0.28ml/s with 22.4psi vacuum 12.2m deep (32.7psia) @20:23:58.78 Cartridge 23 filtering 356/1000ml at 0.29ml/s with 21.7psi vacuum 11.5m deep (31.7psia) @20:24:58.78 Cartridge 23 filtering 373/1000ml at 0.28ml/s with 24.6psi vacuum 12.6m deep (33.3psia) @20:25:58.79 Cartridge 23 filtering 390/1000ml at 0.28ml/s with 26.3psi vacuum 10.7m deep (30.5psia) @20:26:58.78 Cartridge 23 filtering 407/1000ml at 0.27ml/s with 22.0psi vacuum 12.6m deep (33.3psia) @20:27:58.79 Cartridge 23 filtering 424/1000ml at 0.27ml/s with 23.7psi vacuum 12.2m deep (32.7psia) @20:28:58.79 Cartridge 23 filtering 440/1000ml at 0.29ml/s with 23.9psi vacuum 13.1m deep (34.0psia) @20:29:58.79 Cartridge 23 filtering 457/1000ml at 0.29ml/s with 23.3psi vacuum 12.7m deep (33.5psia) @20:30:58.78 Cartridge 23 filtering 473/1000ml at 0.26ml/s with 22.3psi vacuum 13.1m deep (34.0psia) @20:31:58.78 Cartridge 23 filtering 489/1000ml at 0.26ml/s with 24.2psi vacuum 12.2m deep (32.8psia) @20:32:58.78 Cartridge 23 filtering 505/1000ml at 0.28ml/s with 23.1psi vacuum 13.1m deep (34.0psia) @20:33:58.79 Cartridge 23 filtering 521/1000ml at 0.27ml/s with 22.9psi vacuum 11.2m deep (31.3psia) @20:34:58.78 Cartridge 23 filtering 537/1000ml at 0.26ml/s with 24.7psi vacuum 12.5m deep (33.1psia) @20:35:58.78 Cartridge 23 filtering 552/1000ml at 0.25ml/s with 25.0psi vacuum 12.8m deep (33.7psia) @20:36:58.78 Cartridge 23 filtering 568/1000ml at 0.26ml/s with 23.6psi vacuum 12.8m deep (33.6psia) @20:37:58.79 Cartridge 23 filtering 583/1000ml at 0.26ml/s with 23.0psi vacuum 11.8m deep (32.1psia) @20:38:58.78 Cartridge 23 filtering 598/1000ml at 0.25ml/s with 24.3psi vacuum 11.1m deep (31.1psia) @20:39:58.78 Cartridge 23 filtering 613/1000ml at 0.26ml/s with 27.1psi vacuum 11.8m deep (32.2psia) @20:40:58.78 Cartridge 23 filtering 628/1000ml at 0.24ml/s with 23.4psi vacuum 11.9m deep (32.2psia) @20:41:58.78 Cartridge 23 filtering 643/1000ml at 0.26ml/s with 23.1psi vacuum 12.6m deep (33.4psia) @20:42:58.78 Cartridge 23 filtering 658/1000ml at 0.24ml/s with 24.7psi vacuum 12.1m deep (32.6psia) @20:43:58.79 Cartridge 23 filtering 672/1000ml at 0.26ml/s with 23.7psi vacuum 13.5m deep (34.6psia) @20:44:59.16 Cartridge 23 filtering 686/1000ml at 0.22ml/s with 24.7psi vacuum 11.1m deep (31.1psia) @20:45:58.79 Cartridge 23 filtering 701/1000ml at 0.23ml/s with 24.3psi vacuum 13.0m deep (33.9psia) @20:46:58.78 Cartridge 23 filtering 715/1000ml at 0.25ml/s with 25.2psi vacuum 13.3m deep (34.3psia) @20:47:58.79 Cartridge 23 filtering 729/1000ml at 0.23ml/s with 23.8psi vacuum 12.1m deep (32.6psia) @20:48:58.78 Cartridge 23 filtering 743/1000ml at 0.23ml/s with 24.6psi vacuum 11.5m deep (31.6psia) @20:49:58.79 Cartridge 23 filtering 757/1000ml at 0.24ml/s with 24.5psi vacuum 12.4m deep (33.1psia) @20:50:58.78 Cartridge 23 filtering 770/1000ml at 0.22ml/s with 25.7psi vacuum 10.8m deep (30.6psia) @20:51:58.78 Cartridge 23 filtering 784/1000ml at 0.21ml/s with 24.2psi vacuum 11.3m deep (31.4psia) @20:52:58.78 Cartridge 23 filtering 797/1000ml at 0.22ml/s with 24.0psi vacuum 12.2m deep (32.7psia) @20:53:58.78 Cartridge 23 filtering 811/1000ml at 0.22ml/s with 23.6psi vacuum 12.1m deep (32.6psia) @20:54:58.78 Cartridge 23 filtering 824/1000ml at 0.24ml/s with 22.8psi vacuum 12.5m deep (33.2psia) @20:55:58.78 Cartridge 23 filtering 837/1000ml at 0.22ml/s with 22.9psi vacuum 11.9m deep (32.2psia) @20:56:58.78 Cartridge 23 filtering 851/1000ml at 0.21ml/s with 24.1psi vacuum 12.5m deep (33.1psia) @20:57:58.79 Cartridge 23 filtering 864/1000ml at 0.21ml/s with 23.6psi vacuum 11.7m deep (31.9psia) @20:58:58.78 Cartridge 23 filtering 876/1000ml at 0.20ml/s with 24.9psi vacuum 12.5m deep (33.2psia) @20:59:58.78 Cartridge 23 filtering 889/1000ml at 0.23ml/s with 25.4psi vacuum 12.7m deep (33.5psia) @21:00:58.78 Cartridge 23 filtering 902/1000ml at 0.20ml/s with 23.8psi vacuum 12.8m deep (33.6psia) @21:01:58.78 Cartridge 23 filtering 915/1000ml at 0.21ml/s with 23.0psi vacuum 12.3m deep (32.9psia) @21:02:58.78 Cartridge 23 filtering 927/1000ml at 0.20ml/s with 25.6psi vacuum 10.9m deep (30.8psia) @21:03:58.79 Cartridge 23 filtering 940/1000ml at 0.20ml/s with 24.6psi vacuum 10.8m deep (30.7psia) @21:04:58.78 Cartridge 23 filtering 952/1000ml at 0.20ml/s with 23.1psi vacuum 13.3m deep (34.4psia) @21:05:58.78 Cartridge 23 filtering 965/1000ml at 0.23ml/s with 24.9psi vacuum 12.7m deep (33.5psia) @21:06:58.78 Cartridge 23 filtering 977/1000ml at 0.20ml/s with 23.4psi vacuum 12.6m deep (33.3psia) @21:07:58.79 Cartridge 23 filtering 989/1000ml at 0.20ml/s with 26.0psi vacuum 11.8m deep (32.2psia) @21:08:50.88 Pressure.stop @21:08:51.01 Intake.close @21:08:51.04 Exhaust.close @21:08:51.25 Toroid.in :hold @21:08:51.32 Toroid.seek :bypass @21:08:52.95 Toroid.in :default @21:08:53.02 Toroid.seek :clear @21:08:54.63 Sampled 1000.0ml @21:08:56.09 -> Cmd.startProcessing @21:08:57.55 SPR.command \"read_sequences\" @21:08:58.10 SPR.command \"stats_on\" @21:08:58.23 SPR.command \"start 0 1\" @21:08:58.95 SPR.start /var/log/esp/spr23apr2021h.spr.bz2 @21:09:00.95 Handoff.select :waste @21:09:07.64 Syringe tare is 17% of full scale @21:09:07.67 PinPort.in :locking @21:09:07.75 PinPort.seek :locked @21:09:08.23 Archive.in :fast @21:09:08.27 Lysis2.in :fast @21:09:08.36 Lysis1.seek 1350uL @21:09:08.48 Archive.seek :full @21:09:08.52 Lysis2.seek :full @21:09:10.56 Plunge.seek :reagent @21:09:11.45 Evacuating puck with filter bubble point of 50psi @21:09:11.48 PinPort.seek :locked @21:09:11.57 Handoff.select :waste @21:09:18.15 Air syringe pumping up to 1 strokes to reach 8psi @21:09:18.16 Air stroke #1 of 1 @21:09:18.26 Air.in :pressurize @21:09:18.33 Air.seek :pressurized @21:09:24.06 2.1psi after pressurize @21:09:24.11 Air.in :compress @21:09:24.21 Air.seek :evac @21:09:27.21 0.9psi after compress @21:09:37.79 -0.1psi after compress (stable) @21:09:37.80 Air.in :default @21:09:37.88 Air.hold @21:09:38.07 at -0.1psi Opened to waste @21:09:38.08 Handoff.select :blocked @21:09:38.22 Archive syringe pumping up to 8 strokes to reach 50psi @21:09:38.23 Archive stroke #1 of 8 @21:09:38.35 Archive.in :pressurize @21:09:38.44 Archive.seek :pressurized @21:09:50.95 5.1psi after pressurize @21:09:56.52 5.6psi after pressurize (stable) @21:09:56.54 Archive.in :compress @21:09:56.62 Archive.seek :empty @21:10:11.26 33.4psi after compress @21:10:24.34 38.4psi after compress (stable) @21:10:24.36 Archive.in :gulp @21:10:24.45 Archive.seek :topVent @21:10:30.70 38.6psi after gulp @21:10:36.32 38.7psi after gulp (stable) @21:10:36.33 Archive stroke #2 of 8 @21:10:36.43 Archive.in :pressurize @21:10:36.50 Archive.seek :pressurized @21:10:49.01 38.4psi after pressurize @21:10:54.59 38.6psi after pressurize (stable) @21:10:54.61 Archive.in :compress @21:10:54.69 Archive.seek :empty @21:11:07.28 Reached 50.3 of 50.0psi after pumping 3414uL (1.97 strokes) @21:11:07.30 Archive.in :default @21:11:07.38 Archive.hold @21:11:07.46 Handoff.select :waste @21:11:31.15 delay 1:00 @21:12:31.17 Handoff.select :blocked @21:12:53.88 Handoff.select :waste @21:12:54.01 2.0psi at evacEnd @21:12:54.02 Handoff.select :blocked @21:12:54.17 Archive.in :fast @21:12:54.24 Archive.seek :topVent @21:13:07.76 Stable @1.4psi after Handoff blocked @21:13:07.78 Archive syringe pumping up to 8 strokes to reach 31psi @21:13:07.80 Archive stroke #1 of 8 @21:13:07.91 Archive.in :pressurize @21:13:07.98 Archive.seek :pressurized @21:13:20.49 2.0psi after pressurize @21:13:20.51 Archive.in :compress @21:13:20.63 Archive.seek :empty @21:13:35.26 7.3psi after compress @21:13:59.37 15.7psi after compress (stable) @21:13:59.40 Archive.in :gulp @21:13:59.49 Archive.seek :topVent @21:14:05.74 16.4psi after gulp @21:14:11.31 16.8psi after gulp (stable) @21:14:11.33 Archive stroke #2 of 8 @21:14:11.80 Archive.in :pressurize @21:14:11.87 Archive.seek :pressurized @21:14:24.38 17.5psi after pressurize @21:14:24.40 Archive.in :compress @21:14:24.48 Archive.seek :empty @21:14:39.16 21.6psi after compress @21:15:02.76 29.6psi after compress (stable) @21:15:02.80 Archive.in :gulp @21:15:02.89 Archive.seek :topVent @21:15:09.14 30.5psi after gulp @21:15:14.72 30.9psi after gulp (stable) @21:15:14.73 Archive stroke #3 of 8 @21:15:14.83 Archive.in :pressurize @21:15:14.91 Archive.seek :pressurized @21:15:21.99 Reached 31.4 of 31.4psi after pumping 4251uL (2.46 strokes) @21:15:22.00 Archive.in :default @21:15:22.08 Archive.hold @21:15:22.20 Handoff.select :waste @21:15:35.39 delay 1:00 @21:16:35.42 Handoff.select :blocked @21:16:58.14 Handoff.select :waste @21:16:58.27 1.9psi at evacEnd @21:16:58.29 Air.seek :fullVent @21:16:59.72 Archive.in :fast @21:16:59.79 Archive.seek :topVent @21:17:03.65 Seawater evacuated, Pressure above/below filter released. @21:17:03.74 Lysis1.in :slow @21:17:03.85 Lysis1.seek 1050uL @21:17:33.90 delay 5 seconds @21:17:38.92 Handoff.select :blocked @21:17:39.11 Lysis1.seek 450uL @21:18:39.14 delay 3 seconds @21:18:42.49 Air.in mix with 1.40048 parts Lysis1 @21:18:42.96 Air.seek :evac @21:18:43.00 Lysis1.seek 300uL @21:18:58.17 Lysis1.seek :empty @21:19:28.33 Lysis buffer delivered @26.5psi @21:19:28.35 Polling Can every 2:00 @21:19:28.90 PuckHeater.configure :default #ambient 12.2C @21:19:28.95 PuckHeater.seek 80.2C @21:20:38.89 PuckHeater.hold 2:00,80.2C @21:22:39.09 Polling Can every 2:00 @21:22:39.63 PuckHeater.configure :default #ambient 12.2C @21:22:39.69 PuckHeater.hold 30 seconds,79.1C @21:23:09.91 Polling Can every 2:00 @21:23:10.45 PuckHeater.configure :default #ambient 12.2C @21:23:10.51 PuckHeater.hold 30 seconds,78.6C @21:23:40.73 Polling Can every 2:00 @21:23:41.27 PuckHeater.configure :default #ambient 12.2C @21:23:41.33 PuckHeater.hold 10:00,78.1C @21:33:41.82 Lysis complete. Syringe pressure @21.2psi @21:33:41.84 Archive syringe pumping up to 5 strokes to reach 40psi @21:33:41.86 Archive stroke #1 of 5 @21:33:41.96 Archive.in :pressurize @21:33:42.03 Archive.seek :pressurized @21:33:54.53 20.7psi after pressurize @21:34:00.11 20.6psi after pressurize (stable) @21:34:00.13 Archive.in :compress @21:34:00.21 Archive.seek :empty @21:34:07.86 Reached 40.8 of 40.0psi after pumping 1579uL (0.91 strokes) @21:34:07.88 Archive.in :default @21:34:07.95 Archive.hold @21:34:08.02 delay 2 seconds @21:34:10.04 Archive.in :fast @21:34:10.11 Archive.seek :full @21:34:16.76 Lysis1.seek 138uL @21:34:30.65 delay 1 second @21:34:31.68 Archive.seek :full @21:34:31.71 Lysis1.seek :empty @21:34:46.02 Handoff.select :blocked @21:34:46.12 Initial air pressure @46.8psi @21:34:46.18 Lysis1.in :extract @21:34:46.28 Lysis1.seek 600uL,nil @21:34:48.51 Archive syringe pumping up to 6 strokes to reach 25psi @21:34:48.52 Archive stroke #1 of 6 @21:34:48.66 Archive.in :pressurize @21:34:48.73 Archive.seek :pressurized @21:35:01.37 15.9psi after pressurize @21:35:11.95 15.3psi after pressurize (stable) @21:35:11.97 Archive.in :compress @21:35:12.06 Archive.seek :empty @21:35:20.56 Archive.in :default @21:35:20.63 Archive.hold @21:35:20.78 Initial 600uL lysate @16.2psi @21:35:20.79 Handoff.select :blocked @21:35:20.89 Initial air pressure @17.4psi @21:35:20.99 Lysis1.seek 825uL,nil @21:35:22.20 Archive syringe pumping up to 6 strokes to reach 17psi @21:35:22.21 Archive stroke #1 of 6 @21:35:22.31 Archive.in :compress @21:35:22.39 Archive.seek :empty @21:35:26.59 Archive.in :default @21:35:26.66 Archive.hold @21:35:26.91 Recovered 825uL lysate @14.7psi @21:35:26.93 Air.in :halfSpeed @21:35:27.01 Air.seek :fullVent @21:35:29.21 Puck Top Vented, bottom @19.8psi @21:35:29.22 Handoff.select :waste @21:35:29.36 delay 5 seconds @21:35:34.43 Handoff open to waste, bottom @0.0psi @21:35:34.45 Air.in :default @21:35:34.52 Air.seek :evac @21:35:35.94 Lysis2.in :DAmix @21:35:36.44 Lysis1.in :DAmix @21:35:36.83 Lysis2.seek 1480uL @21:35:36.88 Lysis1.seek 575uL @21:36:12.01 delay 5 seconds @21:36:17.04 Handoff.select SPR @21:36:17.17 SPR.command \"start 2 14\" @21:36:18.18 SPR stabilized @21:36:18.32 delay 10 seconds @21:36:28.34 SPR.command \"start 1 0\" @21:36:31.55 Waiting up to 4:00 for SPR to enter state 1 @21:38:35.81 SPR.command \"hold_on\" @21:38:37.71 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @21:38:38.16 Lysis2.seek 1180uL @21:38:38.22 Lysis1.seek 275uL @21:39:20.25 delay 15 seconds @21:39:35.28 SPR.command \"hold_off\" @21:39:38.36 Waiting up to 38:00 for SPR to enter state 7 @21:57:37.46 SPRsummary:18.3RIU,137RIU,146RIU,195RIU,4.75ng/L,1.93ng/L,5.54ng/L after 3083s @22:04:14.69 SPR analysis completed @22:04:19.97 Ended log spr23apr2021h.spr.bz2 @22:04:19.98 SPR.command \"stats_off\" @22:04:21.50 -> Cmd.stop @22:04:21.60 Handoff.select :close @22:04:21.73 Gate.power :heat,:OFF @22:04:21.75 Gate.power :cartridge,:OFF @22:04:21.84 PinPort.in :unlocking @22:04:21.91 PinPort.seek :clear @22:04:22.38 Carousel.in :default @22:04:22.50 Carousel.home.seek 23 @22:04:22.80 Plunge.seek :locked @22:04:23.67 Archive.in :unlocking @22:04:23.80 Archive.seek :clear @22:04:23.84 Air.in :unlocking @22:04:24.03 Air.seek :clear @22:04:24.11 Lysis1.in :unlocking @22:04:24.25 Lysis1.seek :clear @22:04:24.31 Lysis2.in :unlocking @22:04:24.41 Lysis2.seek :clear @22:04:28.34 Gate.power :motor,:OFF @22:04:28.36 Gate.power :core,:OFF @22:04:28.39 Gate.power :analytic1,:OFF @22:04:28.40 Gate.power :analytic2,:OFF @22:04:28.43 Gate.power :analytic3,:OFF @22:04:28.45 Gate.power :main,:OFF @22:04:28.48 Safely stopped and ready to power off ##-- file closed 2023-04-21T14:03:08.726Z