##-- file opened 2023-04-09T23:10:15.355Z @16:10:15.80 -> showStatus @16:10:17.82 -> Cmd.loadCartridge -10 @16:10:17.90 Gate.power :main,:ON,limit to 5000mA @16:10:17.92 Gate.power :analytic1,:ON,limit to 5000mA @16:10:17.94 Gate.power :analytic2,:ON,limit to 5000mA @16:10:17.97 Gate.power :analytic3,:ON,limit to 5000mA @16:10:18.11 Powering core bus @16:10:20.37 SPR Module 1, FW Rev. B, FPGA Rev. C @16:10:20.81 Archive.configure :default @16:10:20.90 Air.configure :default @16:10:20.97 Lysis1.configure :default @16:10:21.04 Lysis2.configure :default @16:10:21.13 Plunge.configure :default @16:10:21.22 SamplePump.configure :default @16:10:21.32 Toroid.configure :default @16:10:21.41 Carousel.configure :default @16:10:21.50 Pressure.configure :default @16:10:21.59 ExtraPump.configure :default @16:10:21.66 PinPort.configure :default @16:10:21.74 All elf controllers running firmware version 4.83 @16:10:21.77 Gate.power :motor,:ON,limit to 5000mA @16:10:22.04 Archive.home.jog 0 @16:10:22.52 Carousel.home.jog 0 @16:10:22.56 Toroid.home.jog 0 @16:10:22.59 Lysis1.home.jog 0 @16:10:22.62 Air.home.jog 0 @16:10:22.65 Lysis2.home.jog 0 @16:10:22.69 PinPort.home.jog 0 @16:10:23.13 Plunge.home.jog 0 @16:10:23.65 Archive.in :homing @16:10:23.94 Archive.jog 1394 @16:10:23.99 Toroid.in :homing @16:10:24.01 Plunge.in :homing @16:10:24.05 Lysis2.in :homing @16:10:24.09 Lysis1.in :homing @16:10:24.12 Air.in :homing @16:10:24.22 PinPort.in :homing @16:10:24.39 Plunge.jog 1572 @16:10:24.41 Toroid.jog 623 @16:10:24.47 Lysis1.jog 1398 @16:10:24.51 Lysis2.jog 1399 @16:10:24.56 Air.jog 1386 @16:10:24.65 PinPort.jog -105 @16:10:24.96 PinPort.setRawPosition! 0 @16:10:25.01 PinPort.seek :home @16:10:25.10 PinPort.in :default @16:10:25.33 Archive.setRawPosition! 50 @16:10:25.43 Archive.seek :home @16:10:25.70 Archive.in :default @16:10:25.78 Plunge.setRawPosition! 50 @16:10:25.86 Plunge.seek :home @16:10:25.95 Lysis1.setRawPosition! 50 @16:10:25.98 Lysis2.setRawPosition! 50 @16:10:26.02 Air.setRawPosition! 50 @16:10:26.08 Lysis1.seek :home @16:10:26.19 Lysis2.seek :home @16:10:26.24 Air.seek :home @16:10:26.37 Plunge.in :default @16:10:26.47 Lysis1.in :default @16:10:26.55 Lysis2.in :default @16:10:26.65 Air.in :default @16:10:26.73 Toroid.setRawPosition! 0 @16:10:26.79 Toroid.seek :home @16:10:26.88 Toroid.in :default @16:10:27.00 Toroid.seek :clear @16:10:28.62 Carousel.in :default @16:10:28.74 Carousel.home.seek 51 @16:10:28.99 Selecting Cartridge 50 @16:10:29.26 Carousel.seek 50 @16:10:29.74 Lysis1.in :fast @16:10:29.82 Lysis1.seek :locked @16:10:32.43 Plunge.seek :locked @16:10:32.56 Air.in :fast @16:10:32.63 Air.seek :locked @16:10:34.20 Carousel.reconfigure :locked @16:10:34.35 Powering cartridge bus @16:10:36.97 Gate.setIR 1750,5000,0,6 @16:10:37.10 Gate.setIR 0,5000,0,6 @16:10:37.16 PuckHeater.configure :default @16:10:37.23 Gate.power :heat,:ON,limit to 5000mA @16:10:37.91 Mounted lysis Cartridge board#Q05062 @16:10:37.95 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:10:55.39 -> Cmd.startFiltering @16:10:56.01 Preparing to filter cartridge 50 @16:10:56.30 Priming sample loop w/100ml, bypass w/10ml @16:10:56.32 SamplePump.setPosition! 0ml @16:10:56.37 Bypass.open @16:10:57.78 Intake.open @16:10:58.52 Sea 8.6m deep (27.4psia) @16:10:58.54 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:11:01.11 Vacuum tare is 59% of full scale @16:11:01.25 Pressure.in :default @16:11:01.32 Pressure.hold @16:11:01.50 SamplePump.in :prime @16:11:01.58 SamplePump.seek 10ml,59 seconds @16:11:10.33 Pressure.stop @16:11:10.38 SamplePump.setPosition! 0ml @16:11:10.44 Exhaust.open @16:11:11.91 Bypass.close @16:11:13.38 Pressure.in :default @16:11:13.45 Pressure.hold @16:11:13.57 Sea 8.4m deep (27.1psia) @16:11:13.72 SamplePump.in :prime @16:11:13.80 SamplePump.seek 100ml,5:16 @16:12:51.84 Pressure.stop @16:12:51.92 Bypass.open @16:12:52.61 Intake.close @16:12:53.37 Toroid.in :hold @16:12:53.44 Toroid.seek :crack @16:12:54.97 Toroid.seek :cartridge @16:12:56.07 Toroid.in :default @16:12:56.14 Toroid.seek :clear @16:12:58.10 Pressurizing cartridge to 26psi above 28.0psia @16:12:58.12 SamplePump.setPosition! 0ml @16:12:58.18 Intake.open @16:12:58.85 Bypass.close @16:12:59.57 Exhaust.close @16:13:00.38 Pressure.in :default @16:13:00.45 Pressure.hold @16:13:00.63 SamplePump.in :charge @16:13:00.71 SamplePump.seek 2.3ml,42 seconds @16:13:10.29 Pressure.stop @16:13:10.35 Intake.close @16:13:11.09 Pressurized cartridge with 0.434ml to 27.0psi @16:13:11.10 Isolated @51.8psia (23.9psi) @16:13:11.12 Verifying cartridge holds at least 0.65..0.85 of 51.8psia @16:13:11.53 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:13:16.19 Sampling 1000.0ml in 1:25:20 @16:13:16.20 SamplePump.setPosition! 0ml @16:13:16.27 Exhaust.open @16:13:16.98 Intake.open @16:13:17.79 Pressure.in :default @16:13:17.86 Pressure.hold @16:13:18.03 Sea 8.8m deep (27.7psia) @16:13:18.04 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @16:13:18.20 SamplePump.in :sample @16:13:18.27 SamplePump.seek 1000ml,1:23:51 @16:13:20.83 Cartridge 50 filtering 1/1000ml at 0.40ml/s with 23.0psi vacuum 8.7m deep (27.5psia) @16:14:20.88 Cartridge 50 filtering 24/1000ml at 0.37ml/s with 24.2psi vacuum 6.9m deep (24.9psia) @16:15:20.83 Cartridge 50 filtering 47/1000ml at 0.36ml/s with 23.7psi vacuum 6.4m deep (24.1psia) @16:16:20.83 Cartridge 50 filtering 69/1000ml at 0.38ml/s with 25.3psi vacuum 6.3m deep (24.0psia) @16:17:20.88 Cartridge 50 filtering 90/1000ml at 0.37ml/s with 22.4psi vacuum 8.1m deep (26.7psia) @16:18:20.83 Cartridge 50 filtering 112/1000ml at 0.33ml/s with 25.4psi vacuum 5.3m deep (22.5psia) @16:19:20.84 Cartridge 50 filtering 132/1000ml at 0.33ml/s with 22.9psi vacuum 7.2m deep (25.3psia) @16:20:20.84 Cartridge 50 filtering 153/1000ml at 0.35ml/s with 24.9psi vacuum 7.6m deep (26.0psia) @16:21:20.84 Cartridge 50 filtering 173/1000ml at 0.32ml/s with 23.2psi vacuum 5.4m deep (22.6psia) @16:22:20.83 Cartridge 50 filtering 192/1000ml at 0.33ml/s with 22.5psi vacuum 6.3m deep (23.9psia) @16:23:20.83 Cartridge 50 filtering 211/1000ml at 0.32ml/s with 24.7psi vacuum 6.7m deep (24.6psia) @16:24:20.83 Cartridge 50 filtering 230/1000ml at 0.29ml/s with 22.9psi vacuum 7.1m deep (25.2psia) @16:25:20.83 Cartridge 50 filtering 249/1000ml at 0.29ml/s with 26.5psi vacuum 5.7m deep (23.1psia) @16:26:20.84 Cartridge 50 filtering 267/1000ml at 0.30ml/s with 26.4psi vacuum 4.1m deep (20.7psia) @16:27:20.83 Cartridge 50 filtering 284/1000ml at 0.30ml/s with 23.5psi vacuum 7.4m deep (25.6psia) @16:28:20.83 Cartridge 50 filtering 302/1000ml at 0.28ml/s with 24.8psi vacuum 4.9m deep (22.0psia) @16:29:20.84 Cartridge 50 filtering 319/1000ml at 0.27ml/s with 22.0psi vacuum 5.3m deep (22.5psia) @16:30:20.83 Cartridge 50 filtering 336/1000ml at 0.27ml/s with 22.7psi vacuum 4.1m deep (20.8psia) @16:31:20.82 Cartridge 50 filtering 353/1000ml at 0.30ml/s with 23.4psi vacuum 7.5m deep (25.8psia) @16:32:20.83 Cartridge 50 filtering 370/1000ml at 0.28ml/s with 22.6psi vacuum 5.7m deep (23.1psia) @16:33:20.83 Cartridge 50 filtering 386/1000ml at 0.28ml/s with 23.4psi vacuum 5.3m deep (22.5psia) @16:34:20.83 Cartridge 50 filtering 403/1000ml at 0.27ml/s with 24.7psi vacuum 5.9m deep (23.4psia) @16:35:20.83 Cartridge 50 filtering 419/1000ml at 0.27ml/s with 24.2psi vacuum 6.0m deep (23.6psia) @16:36:21.18 Cartridge 50 filtering 435/1000ml at 0.26ml/s with 22.4psi vacuum 4.4m deep (21.3psia) @16:37:20.83 Cartridge 50 filtering 451/1000ml at 0.25ml/s with 26.1psi vacuum 5.5m deep (22.8psia) @16:38:20.84 Cartridge 50 filtering 466/1000ml at 0.27ml/s with 23.6psi vacuum 4.2m deep (20.9psia) @16:39:20.84 Cartridge 50 filtering 482/1000ml at 0.26ml/s with 22.6psi vacuum 6.0m deep (23.6psia) @16:40:20.84 Cartridge 50 filtering 497/1000ml at 0.25ml/s with 25.3psi vacuum 4.0m deep (20.6psia) @16:41:20.83 Cartridge 50 filtering 513/1000ml at 0.25ml/s with 24.1psi vacuum 5.3m deep (22.5psia) @16:42:20.84 Cartridge 50 filtering 528/1000ml at 0.26ml/s with 21.5psi vacuum 6.4m deep (24.2psia) @16:43:20.83 Cartridge 50 filtering 543/1000ml at 0.25ml/s with 27.3psi vacuum 3.9m deep (20.4psia) @16:44:20.84 Cartridge 50 filtering 557/1000ml at 0.25ml/s with 23.2psi vacuum 3.7m deep (20.2psia) @16:45:20.84 Cartridge 50 filtering 572/1000ml at 0.24ml/s with 26.5psi vacuum 4.3m deep (21.1psia) @16:46:20.83 Cartridge 50 filtering 587/1000ml at 0.26ml/s with 22.7psi vacuum 5.0m deep (22.1psia) @16:47:20.83 Cartridge 50 filtering 601/1000ml at 0.23ml/s with 23.1psi vacuum 5.1m deep (22.2psia) @16:48:20.82 Cartridge 50 filtering 615/1000ml at 0.23ml/s with 24.2psi vacuum 4.3m deep (21.1psia) @16:49:20.84 Cartridge 50 filtering 629/1000ml at 0.22ml/s with 25.2psi vacuum 3.7m deep (20.2psia) @16:50:20.84 Cartridge 50 filtering 643/1000ml at 0.24ml/s with 22.4psi vacuum 5.5m deep (22.8psia) @16:51:20.84 Cartridge 50 filtering 657/1000ml at 0.24ml/s with 22.8psi vacuum 5.0m deep (22.1psia) @16:52:20.83 Cartridge 50 filtering 671/1000ml at 0.22ml/s with 24.4psi vacuum 4.0m deep (20.5psia) @16:53:20.84 Cartridge 50 filtering 685/1000ml at 0.23ml/s with 23.1psi vacuum 5.2m deep (22.4psia) @16:54:20.84 Cartridge 50 filtering 698/1000ml at 0.22ml/s with 23.5psi vacuum 3.8m deep (20.3psia) @16:55:20.83 Cartridge 50 filtering 712/1000ml at 0.21ml/s with 23.3psi vacuum 4.0m deep (20.6psia) @16:56:20.83 Cartridge 50 filtering 725/1000ml at 0.21ml/s with 24.9psi vacuum 3.8m deep (20.3psia) @16:57:20.83 Cartridge 50 filtering 738/1000ml at 0.24ml/s with 24.2psi vacuum 5.3m deep (22.5psia) @16:58:20.82 Cartridge 50 filtering 751/1000ml at 0.21ml/s with 22.7psi vacuum 4.8m deep (21.8psia) @16:59:20.84 Cartridge 50 filtering 764/1000ml at 0.21ml/s with 23.8psi vacuum 4.9m deep (22.0psia) @17:00:20.83 Cartridge 50 filtering 777/1000ml at 0.21ml/s with 24.6psi vacuum 4.5m deep (21.3psia) @17:01:20.83 Cartridge 50 filtering 790/1000ml at 0.22ml/s with 24.3psi vacuum 4.6m deep (21.4psia) @17:02:20.86 Cartridge 50 filtering 803/1000ml at 0.22ml/s with 22.2psi vacuum 5.2m deep (22.3psia) @17:03:20.83 Cartridge 50 filtering 815/1000ml at 0.21ml/s with 24.1psi vacuum 4.6m deep (21.5psia) @17:04:20.85 Cartridge 50 filtering 828/1000ml at 0.20ml/s with 26.3psi vacuum 3.8m deep (20.3psia) @17:05:20.84 Cartridge 50 filtering 840/1000ml at 0.20ml/s with 22.9psi vacuum 5.0m deep (22.1psia) @17:06:20.83 Cartridge 50 filtering 853/1000ml at 0.21ml/s with 22.9psi vacuum 4.6m deep (21.6psia) @17:07:20.84 Cartridge 50 filtering 865/1000ml at 0.20ml/s with 24.8psi vacuum 4.0m deep (20.6psia) @17:08:20.83 Cartridge 50 filtering 877/1000ml at 0.20ml/s with 24.3psi vacuum 3.8m deep (20.3psia) @17:09:20.84 Cartridge 50 filtering 889/1000ml at 0.20ml/s with 24.8psi vacuum 5.0m deep (22.1psia) @17:10:20.83 Cartridge 50 filtering 901/1000ml at 0.20ml/s with 26.3psi vacuum 4.1m deep (20.8psia) @17:11:20.82 Cartridge 50 filtering 914/1000ml at 0.20ml/s with 24.4psi vacuum 4.4m deep (21.2psia) @17:12:20.83 Cartridge 50 filtering 926/1000ml at 0.20ml/s with 26.0psi vacuum 4.8m deep (21.8psia) @17:13:21.15 Cartridge 50 filtering 938/1000ml at 0.20ml/s with 24.6psi vacuum 5.3m deep (22.5psia) @17:14:20.84 Cartridge 50 filtering 950/1000ml at 0.20ml/s with 26.4psi vacuum 4.6m deep (21.5psia) @17:15:20.84 Cartridge 50 filtering 962/1000ml at 0.20ml/s with 24.7psi vacuum 5.4m deep (22.6psia) @17:16:20.87 Cartridge 50 filtering 974/1000ml at 0.20ml/s with 25.4psi vacuum 3.8m deep (20.3psia) @17:16:53.97 Flow averaged 0.26ml/s over 1:03:36 @17:16:54.02 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi @17:16:54.60 Relieved excess vacuum in .6 seconds @17:16:54.64 SamplePump.seek 1000ml,2:06 @17:17:20.82 Cartridge 50 filtering 986/1000ml at 0.20ml/s with 26.1psi vacuum 4.6m deep (21.5psia) @17:18:20.84 Cartridge 50 filtering 998/1000ml at 0.20ml/s with 25.2psi vacuum 4.2m deep (20.9psia) @17:18:29.40 Pressure.stop @17:18:29.52 Intake.close @17:18:29.57 Exhaust.close @17:18:29.78 Toroid.in :hold @17:18:29.85 Toroid.seek :bypass @17:18:31.48 Toroid.in :default @17:18:31.55 Toroid.seek :clear @17:18:33.17 Sampled 1000.0ml @17:18:34.50 -> Cmd.startProcessing @17:18:35.95 SPR.command \"read_sequences\" @17:18:36.63 SPR.command \"stats_on\" @17:18:36.76 SPR.command \"start 0 1\" @17:18:37.49 SPR.start /var/log/esp/spr23apr0917h.spr.bz2 @17:18:39.48 Handoff.select :waste @17:18:46.16 Syringe tare is 17% of full scale @17:18:46.19 PinPort.in :locking @17:18:46.27 PinPort.seek :locked @17:18:46.76 Archive.in :fast @17:18:46.80 Lysis2.in :fast @17:18:46.90 Lysis1.seek 1350uL @17:18:46.99 Archive.seek :full @17:18:47.08 Lysis2.seek :full @17:18:49.05 Plunge.seek :reagent @17:18:50.37 Evacuating puck with filter bubble point of 50psi @17:18:50.40 PinPort.seek :locked @17:18:50.50 Handoff.select :waste @17:18:57.09 Air syringe pumping up to 1 strokes to reach 8psi @17:18:57.10 Air stroke #1 of 1 @17:18:57.20 Air.in :pressurize @17:18:57.27 Air.seek :pressurized @17:19:03.00 0.3psi after pressurize @17:19:03.02 Air.in :compress @17:19:03.11 Air.seek :evac @17:19:06.11 1.2psi after compress @17:19:16.68 0.1psi after compress (stable) @17:19:16.69 Air.in :default @17:19:16.77 Air.hold @17:19:17.00 at 0.0psi Opened to waste @17:19:17.01 Handoff.select :blocked @17:19:17.15 Archive syringe pumping up to 8 strokes to reach 50psi @17:19:17.16 Archive stroke #1 of 8 @17:19:17.26 Archive.in :pressurize @17:19:17.33 Archive.seek :pressurized @17:19:29.83 9.4psi after pressurize @17:19:52.92 20.6psi after pressurize (stable) @17:19:52.94 Archive.in :compress @17:19:53.03 Archive.seek :empty @17:20:07.66 30.8psi after compress @17:20:18.73 33.4psi after compress (stable) @17:20:18.75 Archive.in :gulp @17:20:18.84 Archive.seek :topVent @17:20:25.09 33.5psi after gulp @17:20:30.67 33.5psi after gulp (stable) @17:20:30.68 Archive stroke #2 of 8 @17:20:30.78 Archive.in :pressurize @17:20:30.86 Archive.seek :pressurized @17:20:43.36 33.3psi after pressurize @17:21:06.43 37.8psi after pressurize (stable) @17:21:06.45 Archive.in :compress @17:21:06.55 Archive.seek :empty @17:21:19.43 Reached 50.2 of 50.0psi after pumping 3421uL (1.98 strokes) @17:21:19.45 Archive.in :default @17:21:19.53 Archive.hold @17:21:19.60 Handoff.select :waste @17:21:27.79 delay 1:00 @17:22:27.80 Handoff.select :blocked @17:22:55.49 Handoff.select :waste @17:22:55.66 3.8psi at evacEnd @17:22:55.67 Handoff.select :blocked @17:22:55.81 Archive.in :fast @17:22:55.88 Archive.seek :topVent @17:23:09.42 Stable @2.2psi after Handoff blocked @17:23:09.44 Archive syringe pumping up to 8 strokes to reach 32psi @17:23:09.46 Archive stroke #1 of 8 @17:23:09.59 Archive.in :pressurize @17:23:09.66 Archive.seek :pressurized @17:23:22.16 3.4psi after pressurize @17:23:22.18 Archive.in :compress @17:23:22.26 Archive.seek :empty @17:23:36.91 8.5psi after compress @17:24:07.00 18.4psi after compress (stable) @17:24:07.02 Archive.in :gulp @17:24:07.11 Archive.seek :topVent @17:24:13.35 19.2psi after gulp @17:24:18.93 19.5psi after gulp (stable) @17:24:18.94 Archive stroke #2 of 8 @17:24:19.04 Archive.in :pressurize @17:24:19.11 Archive.seek :pressurized @17:24:31.60 20.0psi after pressurize @17:24:31.63 Archive.in :compress @17:24:31.71 Archive.seek :empty @17:24:46.34 24.3psi after compress @17:25:08.91 31.4psi after compress (stable) @17:25:08.93 Archive.in :gulp @17:25:09.02 Archive.seek :topVent @17:25:15.36 32.0psi after gulp @17:25:18.04 Reached 32.2 of 32.2psi after pumping 3460uL (2.00 strokes) @17:25:18.05 Archive.in :default @17:25:18.15 Archive.hold @17:25:18.23 Handoff.select :waste @17:25:25.43 delay 1:00 @17:26:25.45 Handoff.select :blocked @17:26:48.14 Handoff.select :waste @17:26:48.27 1.3psi at evacEnd @17:26:48.29 Air.seek :fullVent @17:26:49.72 Archive.in :fast @17:26:49.79 Archive.seek :topVent @17:26:49.89 Seawater evacuated, Pressure above/below filter released. @17:26:50.00 Lysis1.in :slow @17:26:50.07 Lysis1.seek 1050uL @17:27:20.14 delay 5 seconds @17:27:25.17 Handoff.select :blocked @17:27:25.36 Lysis1.seek 450uL @17:28:25.38 delay 3 seconds @17:28:28.48 Air.in mix with 1.40048 parts Lysis1 @17:28:28.93 Air.seek :evac @17:28:28.97 Lysis1.seek 300uL @17:28:44.10 Lysis1.seek :empty @17:29:14.23 Lysis buffer delivered @17.4psi @17:29:14.25 Polling Can every 2:00 @17:29:14.80 PuckHeater.configure :default #ambient 11.6C @17:29:14.85 PuckHeater.seek 80.2C @17:30:28.10 PuckHeater.hold 2:00,80.2C @17:32:28.22 Polling Can every 2:00 @17:32:28.76 PuckHeater.configure :default #ambient 11.7C @17:32:28.82 PuckHeater.hold 30 seconds,79.2C @17:32:59.01 Polling Can every 2:00 @17:32:59.55 PuckHeater.configure :default #ambient 11.7C @17:32:59.61 PuckHeater.hold 30 seconds,78.6C @17:33:29.78 Polling Can every 2:00 @17:33:30.32 PuckHeater.configure :default #ambient 11.7C @17:33:30.38 PuckHeater.hold 10:00,78.1C @17:43:30.16 Lysis complete. Syringe pressure @21.3psi @17:43:30.18 Archive syringe pumping up to 5 strokes to reach 40psi @17:43:30.20 Archive stroke #1 of 5 @17:43:30.30 Archive.in :pressurize @17:43:30.37 Archive.seek :pressurized @17:43:42.89 20.8psi after pressurize @17:43:48.48 20.7psi after pressurize (stable) @17:43:48.50 Archive.in :compress @17:43:48.60 Archive.seek :empty @17:43:56.30 Reached 40.9 of 40.0psi after pumping 1580uL (0.91 strokes) @17:43:56.31 Archive.in :default @17:43:56.39 Archive.hold @17:43:56.46 delay 2 seconds @17:43:58.49 Archive.in :fast @17:43:58.55 Archive.seek :full @17:44:05.20 Lysis1.seek 138uL @17:44:19.08 delay 1 second @17:44:20.11 Archive.seek :full @17:44:20.14 Lysis1.seek :empty @17:44:34.09 Handoff.select :blocked @17:44:34.18 Initial air pressure @46.7psi @17:44:34.24 Lysis1.in :extract @17:44:34.34 Lysis1.seek 600uL,nil @17:44:36.08 Archive syringe pumping up to 6 strokes to reach 25psi @17:44:36.09 Archive stroke #1 of 6 @17:44:36.19 Archive.in :pressurize @17:44:36.28 Archive.seek :pressurized @17:44:48.84 14.1psi after pressurize @17:44:54.41 14.2psi after pressurize (stable) @17:44:54.43 Archive.in :compress @17:44:54.53 Archive.seek :empty @17:45:05.32 Archive.in :default @17:45:05.38 Archive.hold @17:45:05.53 Initial 600uL lysate @16.5psi @17:45:05.54 Handoff.select :blocked @17:45:05.64 Initial air pressure @17.5psi @17:45:05.72 Lysis1.seek 825uL,nil @17:45:06.36 Archive syringe pumping up to 6 strokes to reach 17psi @17:45:06.37 Archive stroke #1 of 6 @17:45:06.47 Archive.in :compress @17:45:06.55 Archive.seek :empty @17:45:10.42 14.8psi after compress @17:45:12.71 Archive.in :default @17:45:12.77 Archive.hold @17:45:13.01 Recovered 825uL lysate @17.1psi @17:45:13.03 Air.in :halfSpeed @17:45:13.11 Air.seek :fullVent @17:45:15.31 Puck Top Vented, bottom @4.7psi @17:45:15.32 Handoff.select :waste @17:45:15.45 delay 5 seconds @17:45:20.52 Handoff open to waste, bottom @-0.0psi @17:45:20.53 Air.in :default @17:45:20.61 Air.seek :evac @17:45:22.02 Lysis2.in :DAmix @17:45:22.14 Lysis1.in :DAmix @17:45:22.68 Lysis2.seek 1480uL @17:45:22.71 Lysis1.seek 575uL @17:45:57.81 delay 5 seconds @17:46:02.84 Handoff.select SPR @17:46:02.99 SPR.command \"start 2 14\" @17:46:04.77 SPR stabilized @17:46:04.91 delay 10 seconds @17:46:14.92 SPR.command \"start 1 0\" @17:46:17.65 Waiting up to 4:00 for SPR to enter state 1 @17:48:21.90 SPR.command \"hold_on\" @17:48:23.33 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @17:48:23.80 Lysis2.seek 1180uL @17:48:23.83 Lysis1.seek 275uL @17:49:05.92 delay 15 seconds @17:49:20.94 SPR.command \"hold_off\" @17:49:23.98 Waiting up to 38:00 for SPR to enter state 7 @18:07:23.56 SPRsummary:42.5RIU,204RIU,195RIU,256RIU,none,none,2.59ng/L after 3091s @18:14:00.78 SPR analysis completed @18:14:05.69 Ended log spr23apr0917h.spr.bz2 @18:14:05.71 SPR.command \"stats_off\" @18:14:07.16 -> Cmd.stop @18:14:07.27 Handoff.select :close @18:14:07.40 Gate.power :heat,:OFF @18:14:07.42 Gate.power :cartridge,:OFF @18:14:07.51 PinPort.in :unlocking @18:14:07.58 PinPort.seek :clear @18:14:08.05 Carousel.in :default @18:14:08.17 Carousel.home.seek 50 @18:14:08.50 Plunge.seek :locked @18:14:09.37 Archive.in :unlocking @18:14:09.51 Air.in :unlocking @18:14:09.55 Archive.seek :clear @18:14:09.72 Air.seek :clear @18:14:09.76 Lysis1.in :unlocking @18:14:09.91 Lysis1.seek :clear @18:14:10.01 Lysis2.in :unlocking @18:14:10.09 Lysis2.seek :clear @18:14:14.27 Gate.power :motor,:OFF @18:14:14.29 Gate.power :core,:OFF @18:14:14.31 Gate.power :analytic1,:OFF @18:14:14.33 Gate.power :analytic2,:OFF @18:14:14.36 Gate.power :analytic3,:OFF @18:14:14.38 Gate.power :main,:OFF @18:14:14.40 Safely stopped and ready to power off ##-- file closed 2023-04-10T02:22:27.081Z ##-- file opened 2023-04-10T02:22:27.083Z @19:22:27.61 -> showStatus @19:22:29.63 -> Cmd.loadCartridge -6 @19:22:29.69 Gate.power :main,:ON,limit to 5000mA @19:22:29.72 Gate.power :analytic1,:ON,limit to 5000mA @19:22:29.75 Gate.power :analytic2,:ON,limit to 5000mA @19:22:29.77 Gate.power :analytic3,:ON,limit to 5000mA @19:22:29.91 Powering core bus @19:22:32.18 SPR Module 1, FW Rev. B, FPGA Rev. C @19:22:32.61 Archive.configure :default @19:22:32.69 Air.configure :default @19:22:32.77 Lysis1.configure :default @19:22:32.83 Lysis2.configure :default @19:22:32.92 Plunge.configure :default @19:22:33.01 SamplePump.configure :default @19:22:33.10 Toroid.configure :default @19:22:33.20 Carousel.configure :default @19:22:33.29 Pressure.configure :default @19:22:33.38 ExtraPump.configure :default @19:22:33.45 PinPort.configure :default @19:22:33.52 All elf controllers running firmware version 4.83 @19:22:33.56 Gate.power :motor,:ON,limit to 5000mA @19:22:33.87 Archive.home.jog 0 @19:22:33.98 Air.home.jog 0 @19:22:34.74 Carousel.home.jog 0 @19:22:34.80 Toroid.home.jog 0 @19:22:34.83 Lysis2.home.jog 0 @19:22:34.89 Lysis1.home.jog 0 @19:22:34.95 PinPort.home.jog 0 @19:22:34.99 Plunge.home.jog 0 @19:22:35.49 Air.in :homing @19:22:35.53 Archive.in :homing @19:22:35.69 Air.jog 1382 @19:22:35.75 Archive.jog 1392 @19:22:35.94 PinPort.in :homing @19:22:35.97 Toroid.in :homing @19:22:36.00 Plunge.in :homing @19:22:36.04 Lysis2.in :homing @19:22:36.07 Lysis1.in :homing @19:22:36.24 PinPort.jog -105 @19:22:36.29 Toroid.jog 625 @19:22:36.32 Plunge.jog 1569 @19:22:36.35 Lysis2.jog 1400 @19:22:36.38 Lysis1.jog 1400 @19:22:36.61 PinPort.setRawPosition! 0 @19:22:36.66 PinPort.seek :home @19:22:36.74 PinPort.in :default @19:22:36.87 Air.setRawPosition! 50 @19:22:36.93 Air.seek :home @19:22:37.02 Archive.setRawPosition! 50 @19:22:37.07 Archive.seek :home @19:22:37.22 Air.in :default @19:22:37.34 Archive.in :default @19:22:37.64 Lysis2.setRawPosition! 50 @19:22:37.71 Lysis2.seek :home @19:22:37.79 Plunge.setRawPosition! 50 @19:22:37.81 Lysis1.setRawPosition! 50 @19:22:37.89 Plunge.seek :home @19:22:37.95 Lysis1.seek :home @19:22:38.07 Lysis2.in :default @19:22:38.18 Plunge.in :default @19:22:38.27 Lysis1.in :default @19:22:38.54 Toroid.setRawPosition! 0 @19:22:38.58 Toroid.seek :home @19:22:38.67 Toroid.in :default @19:22:38.75 Toroid.seek :clear @19:22:40.37 Carousel.in :default @19:22:40.48 Carousel.home.seek 50 @19:22:40.73 Selecting Cartridge 46 @19:22:41.06 Carousel.seek 46 @19:22:42.15 Lysis1.in :fast @19:22:42.22 Lysis1.seek :locked @19:22:44.82 Plunge.seek :locked @19:22:44.95 Air.in :fast @19:22:45.03 Air.seek :locked @19:22:46.60 Carousel.reconfigure :locked @19:22:46.74 Powering cartridge bus @19:22:49.36 Gate.setIR 1750,5000,0,6 @19:22:49.48 Gate.setIR 0,5000,0,6 @19:22:49.54 Gate.power :cartridge,:OFF @19:22:50.08 Mounted archive Cartridge @19:23:06.40 -> Cmd.startFiltering @19:23:07.06 Preparing to filter cartridge 46 @19:23:07.35 Priming sample loop w/100ml, bypass w/10ml @19:23:07.36 SamplePump.setPosition! 0ml @19:23:07.43 Bypass.open @19:23:08.90 Intake.open @19:23:09.63 Sea 7.5m deep (25.8psia) @19:23:09.65 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @19:23:12.26 Vacuum tare is 59% of full scale @19:23:12.39 Pressure.in :default @19:23:12.45 Pressure.hold @19:23:12.64 SamplePump.in :prime @19:23:12.71 SamplePump.seek 10ml,59 seconds @19:23:21.48 Pressure.stop @19:23:21.53 SamplePump.setPosition! 0ml @19:23:21.59 Exhaust.open @19:23:23.03 Bypass.close @19:23:24.54 Pressure.in :default @19:23:24.60 Pressure.hold @19:23:24.72 Sea 7.5m deep (25.7psia) @19:23:24.86 SamplePump.in :prime @19:23:24.94 SamplePump.seek 100ml,5:16 @19:25:02.90 Pressure.stop @19:25:02.98 Bypass.open @19:25:03.67 Intake.close @19:25:04.42 Toroid.in :hold @19:25:04.49 Toroid.seek :crack @19:25:06.02 Toroid.seek :cartridge @19:25:07.12 Toroid.in :default @19:25:07.18 Toroid.seek :clear @19:25:09.15 Pressurizing cartridge to 26psi above 25.7psia @19:25:09.16 SamplePump.setPosition! 0ml @19:25:09.22 Intake.open @19:25:09.88 Bypass.close @19:25:10.55 Exhaust.close @19:25:11.34 Pressure.in :default @19:25:11.40 Pressure.hold @19:25:11.59 SamplePump.in :charge @19:25:11.66 SamplePump.seek 2.3ml,42 seconds @19:25:16.40 Pressure.stop @19:25:16.46 Intake.close @19:25:17.21 Pressurized cartridge with 0.104ml to 26.1psi @19:25:17.22 Isolated @49.2psia (23.6psi) @19:25:17.25 Verifying cartridge holds at least 0.65..0.85 of 49.2psia @19:25:17.28 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @19:25:21.94 Sampling 1000.0ml in 1:25:20 @19:25:22.00 SamplePump.setPosition! 0ml @19:25:22.07 Exhaust.open @19:25:22.78 Intake.open @19:25:23.62 Pressure.in :default @19:25:23.68 Pressure.hold @19:25:23.81 Sea 7.7m deep (26.1psia) @19:25:23.82 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @19:25:23.98 SamplePump.in :sample @19:25:24.05 SamplePump.seek 1000ml,1:23:51 @19:25:26.65 Cartridge 46 filtering 1/1000ml at 0.40ml/s with 26.2psi vacuum 5.2m deep (22.4psia) @19:26:26.65 Cartridge 46 filtering 25/1000ml at 0.39ml/s with 24.3psi vacuum 3.8m deep (20.3psia) @19:27:26.66 Cartridge 46 filtering 48/1000ml at 0.37ml/s with 24.8psi vacuum 5.4m deep (22.6psia) @19:28:26.65 Cartridge 46 filtering 70/1000ml at 0.38ml/s with 21.1psi vacuum 6.5m deep (24.3psia) @19:29:26.66 Cartridge 46 filtering 92/1000ml at 0.38ml/s with 23.2psi vacuum 5.0m deep (22.0psia) @19:30:26.65 Cartridge 46 filtering 114/1000ml at 0.34ml/s with 23.0psi vacuum 3.9m deep (20.4psia) @19:31:26.70 Cartridge 46 filtering 135/1000ml at 0.35ml/s with 26.1psi vacuum 3.9m deep (20.5psia) @19:32:26.66 Cartridge 46 filtering 156/1000ml at 0.31ml/s with 24.9psi vacuum 2.4m deep (18.3psia) @19:33:26.66 Cartridge 46 filtering 176/1000ml at 0.34ml/s with 22.3psi vacuum 5.7m deep (23.1psia) @19:34:26.65 Cartridge 46 filtering 197/1000ml at 0.36ml/s with 23.2psi vacuum 7.0m deep (25.1psia) @19:35:26.66 Cartridge 46 filtering 216/1000ml at 0.30ml/s with 25.9psi vacuum 2.5m deep (18.4psia) @19:36:26.65 Cartridge 46 filtering 236/1000ml at 0.35ml/s with 24.7psi vacuum 6.5m deep (24.3psia) @19:37:26.66 Cartridge 46 filtering 255/1000ml at 0.32ml/s with 25.5psi vacuum 2.8m deep (18.8psia) @19:38:26.65 Cartridge 46 filtering 275/1000ml at 0.29ml/s with 24.6psi vacuum 2.6m deep (18.6psia) @19:39:26.66 Cartridge 46 filtering 294/1000ml at 0.34ml/s with 24.2psi vacuum 6.7m deep (24.6psia) @19:40:26.65 Cartridge 46 filtering 312/1000ml at 0.33ml/s with 24.1psi vacuum 4.0m deep (20.6psia) @19:41:26.66 Cartridge 46 filtering 331/1000ml at 0.31ml/s with 23.4psi vacuum 6.5m deep (24.3psia) @19:42:26.65 Cartridge 46 filtering 349/1000ml at 0.33ml/s with 22.6psi vacuum 5.5m deep (22.9psia) @19:43:26.66 Cartridge 46 filtering 367/1000ml at 0.33ml/s with 22.7psi vacuum 6.8m deep (24.8psia) @19:44:26.65 Cartridge 46 filtering 385/1000ml at 0.27ml/s with 22.5psi vacuum 3.4m deep (19.7psia) @19:45:26.66 Cartridge 46 filtering 403/1000ml at 0.29ml/s with 24.1psi vacuum 6.0m deep (23.6psia) @19:46:26.65 Cartridge 46 filtering 421/1000ml at 0.29ml/s with 21.7psi vacuum 4.8m deep (21.8psia) @19:47:26.66 Cartridge 46 filtering 438/1000ml at 0.29ml/s with 24.4psi vacuum 5.4m deep (22.7psia) @19:48:26.65 Cartridge 46 filtering 455/1000ml at 0.27ml/s with 26.7psi vacuum @19:49:26.65 Cartridge 46 filtering 472/1000ml at 0.30ml/s with 23.6psi vacuum 6.9m deep (24.8psia) @19:50:26.69 Cartridge 46 filtering 489/1000ml at 0.30ml/s with 24.6psi vacuum 3.5m deep (19.9psia) @19:51:26.66 Cartridge 46 filtering 506/1000ml at 0.27ml/s with 26.0psi vacuum 3.2m deep (19.5psia) @19:52:26.66 Cartridge 46 filtering 522/1000ml at 0.26ml/s with 26.2psi vacuum 1.8m deep (17.4psia) @19:53:26.66 Cartridge 46 filtering 538/1000ml at 0.26ml/s with 21.2psi vacuum 6.0m deep (23.5psia) @19:54:26.66 Cartridge 46 filtering 555/1000ml at 0.26ml/s with 23.6psi vacuum 4.3m deep (21.0psia) @19:55:26.66 Cartridge 46 filtering 571/1000ml at 0.26ml/s with 21.7psi vacuum 5.2m deep (22.4psia) @19:56:26.65 Cartridge 46 filtering 586/1000ml at 0.26ml/s with 23.8psi vacuum 2.9m deep (19.0psia) @19:57:26.66 Cartridge 46 filtering 602/1000ml at 0.27ml/s with 24.7psi vacuum 3.4m deep (19.6psia) @19:58:26.66 Cartridge 46 filtering 617/1000ml at 0.26ml/s with 24.9psi vacuum 3.8m deep (20.4psia) @19:59:26.66 Cartridge 46 filtering 633/1000ml at 0.27ml/s with 25.2psi vacuum 2.9m deep (18.9psia) @20:00:26.65 Cartridge 46 filtering 648/1000ml at 0.26ml/s with 20.3psi vacuum 4.3m deep (21.1psia) @20:01:26.66 Cartridge 46 filtering 663/1000ml at 0.24ml/s with 25.8psi vacuum 3.1m deep (19.3psia) @20:02:26.68 Cartridge 46 filtering 678/1000ml at 0.25ml/s with 25.6psi vacuum 3.7m deep (20.2psia) @20:03:26.67 Cartridge 46 filtering 693/1000ml at 0.24ml/s with 24.8psi vacuum 2.6m deep (18.6psia) @20:04:26.66 Cartridge 46 filtering 707/1000ml at 0.26ml/s with 23.0psi vacuum 4.8m deep (21.8psia) @20:05:26.66 Cartridge 46 filtering 722/1000ml at 0.24ml/s with 24.0psi vacuum 3.4m deep (19.7psia) @20:06:26.66 Cartridge 46 filtering 736/1000ml at 0.25ml/s with 24.5psi vacuum 5.2m deep (22.4psia) @20:07:26.66 Cartridge 46 filtering 750/1000ml at 0.25ml/s with 27.7psi vacuum 3.8m deep (20.4psia) @20:08:26.65 Cartridge 46 filtering 764/1000ml at 0.24ml/s with 25.2psi vacuum 3.4m deep (19.6psia) @20:09:26.66 Cartridge 46 filtering 778/1000ml at 0.23ml/s with 27.3psi vacuum 3.6m deep (20.0psia) @20:10:26.65 Cartridge 46 filtering 792/1000ml at 0.25ml/s with 24.3psi vacuum 5.0m deep (22.0psia) @20:11:26.66 Cartridge 46 filtering 806/1000ml at 0.22ml/s with 23.6psi vacuum 4.2m deep (20.9psia) @20:12:26.65 Cartridge 46 filtering 820/1000ml at 0.23ml/s with 22.2psi vacuum 4.1m deep (20.8psia) @20:13:26.66 Cartridge 46 filtering 833/1000ml at 0.21ml/s with 25.3psi vacuum 2.1m deep (17.9psia) @20:14:26.66 Cartridge 46 filtering 847/1000ml at 0.24ml/s with 21.4psi vacuum 5.3m deep (22.6psia) @20:15:26.66 Cartridge 46 filtering 860/1000ml at 0.23ml/s with 24.5psi vacuum 3.6m deep (20.0psia) @20:16:26.65 Cartridge 46 filtering 873/1000ml at 0.22ml/s with 22.6psi vacuum 4.5m deep (21.4psia) @20:17:26.67 Cartridge 46 filtering 886/1000ml at 0.20ml/s with 25.8psi vacuum 1.6m deep (17.1psia) @20:18:26.65 Cartridge 46 filtering 899/1000ml at 0.21ml/s with 24.5psi vacuum 1.8m deep (17.4psia) @20:19:26.66 Cartridge 46 filtering 912/1000ml at 0.23ml/s with 21.6psi vacuum 5.8m deep (23.3psia) @20:20:26.65 Cartridge 46 filtering 925/1000ml at 0.20ml/s with 25.9psi vacuum 2.7m deep (18.6psia) @20:21:26.66 Cartridge 46 filtering 937/1000ml at 0.21ml/s with 26.0psi vacuum 4.3m deep (21.1psia) @20:22:26.66 Cartridge 46 filtering 950/1000ml at 0.20ml/s with 23.3psi vacuum 3.6m deep (20.0psia) @20:23:26.66 Cartridge 46 filtering 963/1000ml at 0.21ml/s with 26.4psi vacuum 3.6m deep (20.0psia) @20:24:26.65 Cartridge 46 filtering 975/1000ml at 0.20ml/s with 23.4psi vacuum 3.1m deep (19.2psia) @20:25:26.66 Cartridge 46 filtering 988/1000ml at 0.20ml/s with 25.1psi vacuum 3.2m deep (19.4psia) @20:26:26.65 Cartridge 46 filtering 1000/1000ml at 0.20ml/s with 24.2psi vacuum 3.7m deep (20.1psia) @20:26:27.54 Pressure.stop @20:26:27.73 Intake.close @20:26:27.78 Exhaust.close @20:26:28.03 Toroid.in :hold @20:26:28.11 Toroid.seek :bypass @20:26:29.73 Toroid.in :default @20:26:29.81 Toroid.seek :clear @20:26:31.42 Sampled 1000.0ml @20:26:33.01 -> Cmd.startProcessing @20:26:33.50 Plunge.seek :reagent @20:26:34.30 Sample loop closed. Ready to Deliver Reagents @20:26:34.31 Archive.seek :sealed @20:26:38.26 Archive.seek :evac @20:26:52.38 delay 1:00 @20:27:52.41 Archive.seek :clear @20:28:09.93 RNAlater soak for 10:00 @20:38:09.96 Archive.seek :tVent @20:38:12.40 delay 1 second @20:38:13.42 Archive.seek :evac @20:38:28.96 delay 10 seconds @20:38:38.99 Sample preserved and evacuated @20:38:40.23 -> Cmd.stop @20:38:40.37 Carousel.in :default @20:38:40.48 Carousel.home.seek 46 @20:38:40.77 Plunge.seek :locked @20:38:41.65 Archive.in :unlocking @20:38:41.76 Archive.seek :clear @20:38:41.86 Air.in :unlocking @20:38:42.00 Air.seek :clear @20:38:42.04 Lysis1.in :unlocking @20:38:42.21 Lysis1.seek :clear @20:38:46.29 Gate.power :motor,:OFF @20:38:46.31 Gate.power :core,:OFF @20:38:46.33 Gate.power :analytic1,:OFF @20:38:46.36 Gate.power :analytic2,:OFF @20:38:46.38 Gate.power :analytic3,:OFF @20:38:46.40 Gate.power :main,:OFF @20:38:46.42 Safely stopped and ready to power off ##-- file closed 2023-04-10T16:26:52.271Z