##-- file opened 2022-06-02T22:28:51.094Z
@15:28:51.52 -> showStatus
@15:28:53.13 -> Cmd.loadCartridge -6
@15:28:53.20 Gate.power :main,:ON,limit to 5000mA
@15:28:53.22 Gate.power :analytic1,:ON,limit to 5000mA
@15:28:53.24 Gate.power :analytic2,:ON,limit to 5000mA
@15:28:53.26 Gate.power :analytic3,:ON,limit to 5000mA
@15:28:53.41 Powering core bus
@15:28:55.67 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@15:28:56.13 <LRAUV> Archive.configure :default
@15:28:56.25 Air.configure :default
@15:28:56.37 Lysis1.configure :default
@15:28:56.59 Lysis2.configure :default
@15:28:56.83 Plunge.configure :default
@15:28:57.13 SamplePump.configure :default
@15:28:57.45 Toroid.configure :default
@15:28:57.77 Carousel.configure :default
@15:28:57.96 Pressure.configure :default
@15:28:58.08 ExtraPump.configure :default
@15:28:58.18 PinPort.configure :default
@15:28:58.24 All elf controllers running firmware version 4.79
@15:28:58.27 Gate.power :motor,:ON,limit to 5000mA
@15:28:58.45 <Archive_home> Archive.in :default
@15:28:58.71 Archive.home.jog 0
@15:28:58.85 <Toroid_home> Toroid.in :default
@15:28:58.93 <Plunge_home> Plunge.in :default
@15:28:58.97 <Lysis1_home> Lysis1.in :default
@15:28:59.03 <Air_home> Air.in :default
@15:28:59.08 <PinPort_home> PinPort.in :default
@15:28:59.12 <Lysis2_home> Lysis2.in :default
@15:28:59.30 <Toroid_home> Toroid.home.jog 0
@15:28:59.35 <Lysis1_home> Lysis1.home.jog 0
@15:28:59.37 <Plunge_home> Plunge.home.jog 0
@15:28:59.40 <Air_home> Air.home.jog 0
@15:28:59.42 <PinPort_home> PinPort.home.jog 0
@15:28:59.45 <Lysis2_home> Lysis2.home.jog 0
@15:28:59.99 <Archive_home> Archive.in :homing
@15:29:00.19 <Toroid_home> Toroid.in :homing
@15:29:00.23 <Plunge_home> Plunge.in :homing
@15:29:00.26 <Archive_home> Archive.jog 1391
@15:29:00.30 <PinPort_home> PinPort.in :homing
@15:29:00.34 <Lysis1_home> Lysis1.in :homing
@15:29:00.37 <Air_home> Air.in :homing
@15:29:00.41 <Lysis2_home> Lysis2.in :homing
@15:29:00.59 <Plunge_home> Plunge.jog 1555
@15:29:00.67 <Toroid_home> Toroid.jog 627
@15:29:00.71 <Lysis2_home> Lysis2.jog 1393
@15:29:00.74 <Lysis1_home> Lysis1.jog 1380
@15:29:00.77 <Air_home> Air.jog 1389
@15:29:00.80 <PinPort_home> PinPort.jog -107
@15:29:01.16 PinPort.setRawPosition! 0
@15:29:01.21 PinPort.seek :home
@15:29:01.30 PinPort.in :default
@15:29:01.64 <Archive_home> Archive.setRawPosition! 50
@15:29:01.69 Archive.seek :home
@15:29:01.97 Archive.in :default
@15:29:02.09 <Plunge_home> Plunge.setRawPosition! 50
@15:29:02.12 <Lysis2_home> Lysis2.setRawPosition! 50
@15:29:02.14 <Lysis1_home> Lysis1.setRawPosition! 50
@15:29:02.18 <Air_home> Air.setRawPosition! 50
@15:29:02.27 <Plunge_home> Plunge.seek :home
@15:29:02.36 <Lysis2_home> Lysis2.seek :home
@15:29:02.38 <Air_home> Air.seek :home
@15:29:02.41 <Lysis1_home> Lysis1.seek :home
@15:29:02.70 <Plunge_home> Plunge.in :default
@15:29:02.82 <Lysis2_home> Lysis2.in :default
@15:29:02.85 <Lysis1_home> Lysis1.in :default
@15:29:02.88 <Air_home> Air.in :default
@15:29:03.06 <Toroid_home> Toroid.setRawPosition! 0
@15:29:03.10 Toroid.seek :home
@15:29:03.21 Toroid.in :default
@15:29:03.36 <LRAUV> Toroid.seek :clear
@15:29:04.97 Carousel.in :default
@15:29:05.03 Carousel.home.jog 0
@15:29:05.51 Carousel.in :default
@15:29:05.57 Carousel.seek 31
@15:29:05.74 Selecting Cartridge 30
@15:29:05.99 Carousel.seek 30
@15:29:06.86 Lysis1.in :fast
@15:29:06.92 Lysis1.seek :locked
@15:29:09.53 <Plunge> Plunge.seek :locked
@15:29:09.66 <Air> Air.in :fast
@15:29:09.73 Air.seek :locked
@15:29:12.18 <LRAUV> Powering cartridge bus
@15:29:14.78 Gate.setIR 1750,5000,0,6
@15:29:14.90 Gate.setIR 0,5000,0,6
@15:29:14.96 Gate.power :cartridge,:OFF
@15:29:15.49 Mounted archive Cartridge
@15:29:32.75 -> Cmd.startFiltering
@15:29:32.99 <FILTERING> Preparing to filter cartridge 30
@15:29:33.26 Priming sample loop w/100ml, bypass w/10ml
@15:29:33.28 SamplePump.setPosition! 0ml
@15:29:33.33 Bypass.open
@15:29:34.80 Intake.open
@15:29:35.52 Sea 2.8m deep (18.8psia)
@15:29:35.54 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@15:29:38.10 Vacuum tare is 54% of full scale
@15:29:38.23 Pressure.in :default
@15:29:38.29 Pressure.hold
@15:29:38.47 SamplePump.in :prime
@15:29:38.54 SamplePump.seek 10ml,59 seconds
@15:29:47.15 Pressure.stop
@15:29:47.20 SamplePump.setPosition! 0ml
@15:29:47.27 Exhaust.open
@15:29:48.73 <closeBypass> Bypass.close
@15:29:50.26 <FILTERING> Pressure.in :default
@15:29:50.32 Pressure.hold
@15:29:50.44 Sea 2.8m deep (18.8psia)
@15:29:50.58 SamplePump.in :prime
@15:29:50.65 SamplePump.seek 100ml,5:16
@15:31:23.45 Pressure.stop
@15:31:23.51 Bypass.open
@15:31:24.20 Intake.close
@15:31:24.91 Toroid.in :hold
@15:31:24.97 Toroid.seek :crack
@15:31:26.50 Toroid.seek :cartridge
@15:31:27.59 Toroid.in :default
@15:31:27.65 Toroid.seek :clear
@15:31:29.62 Pressurizing cartridge to 26psi above 18.9psia
@15:31:29.63 SamplePump.setPosition! 0ml
@15:31:29.69 Intake.open
@15:31:30.36 Bypass.close
@15:31:31.04 Exhaust.close
@15:31:31.87 Pressure.in :default
@15:31:31.93 Pressure.hold
@15:31:32.11 SamplePump.in :charge
@15:31:32.17 SamplePump.seek 2.3ml,42 seconds
@15:31:41.04 Pressure.stop
@15:31:41.10 Intake.close
@15:31:41.81 Pressurized cartridge with 0.377ml to 27.0psi
@15:31:41.83 Isolated @42.8psia (23.9psi)
@15:31:41.84 Verifying cartridge holds at least 0.65..0.85 of 42.8psia
@15:31:41.88 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@15:31:46.52 Sampling 1000.0ml
@15:31:46.54 SamplePump.setPosition! 0ml
@15:31:46.60 Exhaust.open
@15:31:47.36 Intake.open
@15:31:48.12 Pressure.in :default
@15:31:48.18 Pressure.hold
@15:31:48.30 Sea 2.9m deep (19.0psia)
@15:31:48.44 SamplePump.in :sample
@15:31:48.51 SamplePump.seek 1000ml,1:23:48
@15:31:51.15 <sampling> Cartridge 30 filtering 1/1000ml at 0.45ml/s with 20.7psi vacuum 2.6m deep (18.6psia)
@15:32:51.16 Cartridge 30 filtering 28/1000ml at 0.47ml/s with 24.2psi vacuum 2.1m deep (17.8psia)
@15:33:51.16 Cartridge 30 filtering 54/1000ml at 0.45ml/s with 25.7psi vacuum 3.5m deep (19.8psia)
@15:34:51.15 Cartridge 30 filtering 79/1000ml at 0.39ml/s with 23.2psi vacuum 2.9m deep (19.0psia)
@15:35:51.16 Cartridge 30 filtering 103/1000ml at 0.40ml/s with 25.6psi vacuum 3.5m deep (19.8psia)
@15:36:51.15 Cartridge 30 filtering 126/1000ml at 0.38ml/s with 26.8psi vacuum 3.4m deep (19.8psia)
@15:37:51.16 Cartridge 30 filtering 148/1000ml at 0.36ml/s with 25.0psi vacuum 3.3m deep (19.5psia)
@15:38:51.15 Cartridge 30 filtering 169/1000ml at 0.32ml/s with 23.7psi vacuum 2.6m deep (18.6psia)
@15:39:51.16 Cartridge 30 filtering 189/1000ml at 0.33ml/s with 24.9psi vacuum 2.5m deep (18.4psia)
@15:40:51.15 Cartridge 30 filtering 209/1000ml at 0.30ml/s with 21.4psi vacuum 3.1m deep (19.2psia)
@15:41:51.16 Cartridge 30 filtering 227/1000ml at 0.29ml/s with 23.2psi vacuum 3.1m deep (19.3psia)
@15:42:51.15 Cartridge 30 filtering 245/1000ml at 0.31ml/s with 21.5psi vacuum 3.8m deep (20.2psia)
@15:43:51.16 Cartridge 30 filtering 262/1000ml at 0.28ml/s with 24.2psi vacuum 4.1m deep (20.8psia)
@15:44:51.15 Cartridge 30 filtering 279/1000ml at 0.30ml/s with 24.4psi vacuum 3.7m deep (20.1psia)
@15:45:51.16 Cartridge 30 filtering 294/1000ml at 0.24ml/s with 21.9psi vacuum 2.1m deep (17.8psia)
@15:46:51.15 Cartridge 30 filtering 309/1000ml at 0.27ml/s with 26.2psi vacuum 3.2m deep (19.5psia)
@15:47:51.16 Cartridge 30 filtering 323/1000ml at 0.23ml/s with 25.7psi vacuum 2.8m deep (18.8psia)
@15:48:51.15 Cartridge 30 filtering 337/1000ml at 0.25ml/s with 24.0psi vacuum 2.5m deep (18.3psia)
@15:49:51.16 Cartridge 30 filtering 350/1000ml at 0.20ml/s with 23.6psi vacuum 2.4m deep (18.2psia)
@15:50:51.15 Cartridge 30 filtering 363/1000ml at 0.20ml/s with 23.1psi vacuum 3.2m deep (19.4psia)
@15:51:51.17 Cartridge 30 filtering 375/1000ml at 0.20ml/s with 25.6psi vacuum 3.7m deep (20.2psia)
@15:52:09.56 <FILTERING> Flow averaged 0.31ml/s over 20:22
@15:52:09.59 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi
@15:52:10.17 Relieved excess vacuum in .6 seconds
@15:52:10.19 SamplePump.seek 1000ml,52:16
@15:52:51.16 <sampling> Cartridge 30 filtering 387/1000ml at 0.20ml/s with 25.7psi vacuum 2.1m deep (17.7psia)
@15:52:54.15 <FILTERING> Flow averaged 0.20ml/s over 44 seconds
@15:52:54.18 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@15:52:54.75 Relieved excess vacuum in .6 seconds
@15:52:54.78 SamplePump.seek 1000ml,51:32
@15:53:01.52 Flow estimate reduced to 0.16ml/s
@15:53:01.54 <closeIntake> Intake.close
@15:53:01.59 <closeExhaust> Exhaust.close
@15:53:01.79 <FILTERING> Toroid.in :hold
@15:53:01.86 Toroid.seek :bypass
@15:53:03.47 Toroid.in :default
@15:53:03.54 Toroid.seek :clear
@15:53:05.14 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@15:53:05.16 Pressure.stop
@15:53:05.34 Sampled  388.8ml
@15:53:07.40 <LRAUV> -> Cmd.startProcessing
@15:53:08.22 <PROCESSING> Plunge.seek :reagent
@15:53:09.02 Sample loop closed. Ready to Deliver Reagents
@15:53:09.03 Archive.seek :sealed
@15:53:12.97 Archive.seek :evac
@15:53:27.01 delay 1:00
@15:54:27.04 Archive.seek :clear
@15:54:44.52 RNAlater soak for 10:00
@16:04:44.55 Archive.seek :tVent
@16:04:46.99 delay 1 second
@16:04:48.01 Archive.seek :evac
@16:05:03.51 delay 10 seconds
@16:05:13.54 Sample preserved and evacuated
@16:05:14.63 <LRAUV> -> Cmd.stop
@16:05:14.78 Carousel.seek 30
@16:05:14.96 Plunge.seek :locked
@16:05:15.88 <Archive> Archive.in :unlocking
@16:05:16.01 <Air> Air.in :unlocking
@16:05:16.05 <Archive> Archive.seek :clear
@16:05:16.20 <Air> Air.seek :clear
@16:05:16.24 <Lysis1> Lysis1.in :unlocking
@16:05:16.41 Lysis1.seek :clear
@16:05:20.21 <LRAUV> Gate.power :motor,:OFF
@16:05:20.23 Gate.power :core,:OFF
@16:05:20.25 Gate.power :analytic1,:OFF
@16:05:20.28 Gate.power :analytic2,:OFF
@16:05:20.30 Gate.power :analytic3,:OFF
@16:05:20.32 Gate.power :main,:OFF
@16:05:20.34 Safely stopped and ready to power off
##-- file closed 2022-06-02T23:17:05.218Z

##-- file opened 2022-06-02T23:17:05.234Z
@16:17:05.68 -> showStatus
@16:17:07.70 -> Cmd.loadCartridge -10
@16:17:07.77 Gate.power :main,:ON,limit to 5000mA
@16:17:07.79 Gate.power :analytic1,:ON,limit to 5000mA
@16:17:07.81 Gate.power :analytic2,:ON,limit to 5000mA
@16:17:07.84 Gate.power :analytic3,:ON,limit to 5000mA
@16:17:07.98 Powering core bus
@16:17:10.24 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@16:17:10.72 <LRAUV> Archive.configure :default
@16:17:10.85 Air.configure :default
@16:17:10.95 Lysis1.configure :default
@16:17:11.05 Lysis2.configure :default
@16:17:11.18 Plunge.configure :default
@16:17:11.32 SamplePump.configure :default
@16:17:11.44 Toroid.configure :default
@16:17:11.57 Carousel.configure :default
@16:17:11.69 Pressure.configure :default
@16:17:11.82 ExtraPump.configure :default
@16:17:11.92 PinPort.configure :default
@16:17:11.98 All elf controllers running firmware version 4.79
@16:17:12.01 Gate.power :motor,:ON,limit to 5000mA
@16:17:12.21 <Archive_home> Archive.in :default
@16:17:12.50 Archive.home.jog 0
@16:17:12.66 <Toroid_home> Toroid.in :default
@16:17:12.74 <Plunge_home> Plunge.in :default
@16:17:12.77 <Lysis1_home> Lysis1.in :default
@16:17:12.83 <Air_home> Air.in :default
@16:17:12.88 <PinPort_home> PinPort.in :default
@16:17:12.91 <Lysis2_home> Lysis2.in :default
@16:17:13.09 <Toroid_home> Toroid.home.jog 0
@16:17:13.16 <PinPort_home> PinPort.home.jog 0
@16:17:13.18 <Lysis2_home> Lysis2.home.jog 0
@16:17:13.21 <Lysis1_home> Lysis1.home.jog 0
@16:17:13.23 <Air_home> Air.home.jog 0
@16:17:13.26 <Plunge_home> Plunge.home.jog 0
@16:17:13.72 <Archive_home> Archive.in :homing
@16:17:14.01 <Toroid_home> Toroid.in :homing
@16:17:14.05 <Plunge_home> Plunge.in :homing
@16:17:14.08 <Archive_home> Archive.jog 1391
@16:17:14.13 <PinPort_home> PinPort.in :homing
@16:17:14.16 <Lysis1_home> Lysis1.in :homing
@16:17:14.20 <Air_home> Air.in :homing
@16:17:14.23 <Lysis2_home> Lysis2.in :homing
@16:17:14.59 <Toroid_home> Toroid.jog 626
@16:17:14.68 <Plunge_home> Plunge.jog 1559
@16:17:14.74 <PinPort_home> PinPort.jog -107
@16:17:14.80 <Lysis1_home> Lysis1.jog 1380
@16:17:14.89 <Lysis2_home> Lysis2.jog 1392
@16:17:14.98 <Air_home> Air.jog 1382
@16:17:15.67 <Archive_home> Archive.setRawPosition! 50
@16:17:15.71 <PinPort_home> PinPort.setRawPosition! 0
@16:17:15.88 <Archive_home> Archive.seek :home
@16:17:15.90 <PinPort_home> PinPort.seek :home
@16:17:16.06 PinPort.in :default
@16:17:16.20 <Archive_home> Archive.in :default
@16:17:16.37 <Plunge_home> Plunge.setRawPosition! 50
@16:17:16.43 Plunge.seek :home
@16:17:16.53 <Lysis1_home> Lysis1.setRawPosition! 50
@16:17:16.55 <Lysis2_home> Lysis2.setRawPosition! 50
@16:17:16.64 <Lysis1_home> Lysis1.seek :home
@16:17:16.67 <Lysis2_home> Lysis2.seek :home
@16:17:16.83 <Plunge_home> Plunge.in :default
@16:17:16.85 <Air_home> Air.setRawPosition! 50
@16:17:16.93 Air.seek :home
@16:17:17.04 <Lysis2_home> Lysis2.in :default
@16:17:17.07 <Lysis1_home> Lysis1.in :default
@16:17:17.19 <Toroid_home> Toroid.setRawPosition! 0
@16:17:17.26 Toroid.seek :home
@16:17:17.36 Toroid.in :default
@16:17:17.38 <Air_home> Air.in :default
@16:17:17.80 <LRAUV> Toroid.seek :clear
@16:17:19.41 Carousel.in :default
@16:17:19.48 Carousel.home.jog 0
@16:17:19.96 Carousel.in :default
@16:17:20.02 Carousel.seek 30
@16:17:20.18 Selecting Cartridge 29
@16:17:20.43 Carousel.seek 29
@16:17:21.29 Lysis1.in :fast
@16:17:21.36 Lysis1.seek :locked
@16:17:23.96 <Plunge> Plunge.seek :locked
@16:17:24.09 <Air> Air.in :fast
@16:17:24.15 Air.seek :locked
@16:17:26.62 <LRAUV> Powering cartridge bus
@16:17:29.22 Gate.setIR 1750,5000,0,6
@16:17:29.34 Gate.setIR 0,5000,0,6
@16:17:29.40 PuckHeater.configure :default
@16:17:29.46 Gate.power :heat,:ON,limit to 5000mA
@16:17:30.14 Mounted lysis Cartridge board#Q05037
@16:17:30.17 Using default puck heat loss curve for HeatingAccelInCarousel
@16:17:48.13 -> Cmd.startFiltering
@16:17:48.37 <FILTERING> Preparing to filter cartridge 29
@16:17:48.64 Priming sample loop w/100ml, bypass w/10ml
@16:17:48.66 SamplePump.setPosition! 0ml
@16:17:48.71 Bypass.open
@16:17:50.16 Intake.open
@16:17:50.91 Sea 2.7m deep (18.6psia)
@16:17:50.93 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@16:17:53.51 Vacuum tare is 54% of full scale
@16:17:53.64 Pressure.in :default
@16:17:53.70 Pressure.hold
@16:17:53.88 SamplePump.in :prime
@16:17:53.95 SamplePump.seek 10ml,59 seconds
@16:18:02.56 Pressure.stop
@16:18:02.61 SamplePump.setPosition! 0ml
@16:18:02.67 Exhaust.open
@16:18:04.12 <closeBypass> Bypass.close
@16:18:05.61 <FILTERING> Pressure.in :default
@16:18:05.67 Pressure.hold
@16:18:05.79 Sea 2.7m deep (18.7psia)
@16:18:05.94 SamplePump.in :prime
@16:18:06.01 SamplePump.seek 100ml,5:16
@16:19:38.79 Pressure.stop
@16:19:38.84 Bypass.open
@16:19:39.48 Intake.close
@16:19:40.22 Toroid.in :hold
@16:19:40.29 Toroid.seek :crack
@16:19:41.79 Toroid.seek :cartridge
@16:19:42.89 Toroid.in :default
@16:19:42.94 Toroid.seek :clear
@16:19:44.90 Pressurizing cartridge to 26psi above 18.2psia
@16:19:44.92 SamplePump.setPosition! 0ml
@16:19:44.97 Intake.open
@16:19:45.63 Bypass.close
@16:19:46.28 Exhaust.close
@16:19:47.10 Pressure.in :default
@16:19:47.16 Pressure.hold
@16:19:47.33 SamplePump.in :charge
@16:19:47.40 SamplePump.seek 2.3ml,42 seconds
@16:19:58.19 Pressure.stop
@16:19:58.25 Intake.close
@16:19:59.00 Pressurized cartridge with 0.561ml to 26.0psi
@16:19:59.01 Isolated @41.3psia (23.1psi)
@16:19:59.03 Verifying cartridge holds at least 0.65..0.85 of 41.3psia
@16:19:59.06 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@16:20:03.70 Sampling 1000.0ml
@16:20:03.72 SamplePump.setPosition! 0ml
@16:20:03.78 Exhaust.open
@16:20:04.52 Intake.open
@16:20:05.39 Pressure.in :default
@16:20:05.45 Pressure.hold
@16:20:05.56 Sea 3.0m deep (19.2psia)
@16:20:05.70 SamplePump.in :sample
@16:20:05.77 SamplePump.seek 1000ml,1:23:48
@16:20:08.37 <sampling> Cartridge 29 filtering 1/1000ml at 0.43ml/s with 25.7psi vacuum 3.3m deep (19.6psia)
@16:21:08.37 Cartridge 29 filtering 27/1000ml at 0.44ml/s with 22.9psi vacuum 2.9m deep (18.9psia)
@16:22:08.37 Cartridge 29 filtering 52/1000ml at 0.41ml/s with 22.1psi vacuum 1.7m deep (17.2psia)
@16:23:08.37 Cartridge 29 filtering 75/1000ml at 0.39ml/s with 25.7psi vacuum 2.4m deep (18.2psia)
@16:24:08.38 Cartridge 29 filtering 98/1000ml at 0.38ml/s with 22.2psi vacuum 3.6m deep (20.0psia)
@16:25:08.37 Cartridge 29 filtering 119/1000ml at 0.35ml/s with 27.8psi vacuum
@16:26:08.38 Cartridge 29 filtering 140/1000ml at 0.36ml/s with 22.1psi vacuum 3.4m deep (19.7psia)
@16:27:08.38 Cartridge 29 filtering 160/1000ml at 0.34ml/s with 24.7psi vacuum
@16:28:08.38 Cartridge 29 filtering 179/1000ml at 0.33ml/s with 21.6psi vacuum 2.3m deep (18.1psia)
@16:29:08.37 Cartridge 29 filtering 197/1000ml at 0.27ml/s with 23.1psi vacuum 2.4m deep (18.2psia)
@16:30:08.38 Cartridge 29 filtering 214/1000ml at 0.30ml/s with 21.7psi vacuum 2.6m deep (18.5psia)
@16:31:08.37 Cartridge 29 filtering 231/1000ml at 0.29ml/s with 25.5psi vacuum 3.0m deep (19.1psia)
@16:32:08.38 Cartridge 29 filtering 247/1000ml at 0.25ml/s with 24.1psi vacuum 2.6m deep (18.5psia)
@16:33:08.37 Cartridge 29 filtering 263/1000ml at 0.24ml/s with 23.9psi vacuum 2.3m deep (18.0psia)
@16:34:08.38 Cartridge 29 filtering 278/1000ml at 0.25ml/s with 21.9psi vacuum 1.7m deep (17.1psia)
@16:35:08.37 Cartridge 29 filtering 292/1000ml at 0.25ml/s with 25.6psi vacuum
@16:36:08.38 Cartridge 29 filtering 306/1000ml at 0.21ml/s with 23.2psi vacuum 2.3m deep (18.1psia)
@16:37:08.37 Cartridge 29 filtering 319/1000ml at 0.20ml/s with 24.9psi vacuum
@16:38:08.38 Cartridge 29 filtering 331/1000ml at 0.20ml/s with 25.0psi vacuum 1.7m deep (17.2psia)
@16:39:08.38 Cartridge 29 filtering 344/1000ml at 0.20ml/s with 25.0psi vacuum 2.5m deep (18.4psia)
@16:39:39.44 <FILTERING> Flow averaged 0.30ml/s over 19:34
@16:39:39.47 Waiting up to 13 seconds for vacuum to reduce from 28.2 to 18.0psi
@16:39:40.05 Relieved excess vacuum in .6 seconds
@16:39:40.07 SamplePump.seek 1000ml,54:40
@16:40:08.37 <sampling> Cartridge 29 filtering 356/1000ml at 0.21ml/s with 24.7psi vacuum 2.2m deep (17.9psia)
@16:40:10.94 <FILTERING> Flow averaged 0.20ml/s over 31 seconds
@16:40:10.97 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi
@16:40:11.55 Relieved excess vacuum in .6 seconds
@16:40:11.57 SamplePump.seek 1000ml,54:08
@16:40:26.08 Flow averaged 0.20ml/s over 15 seconds
@16:40:26.11 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi
@16:40:26.69 Relieved excess vacuum in .6 seconds
@16:40:26.71 SamplePump.seek 1000ml,53:54
@16:40:38.26 Flow averaged 0.20ml/s over 12 seconds
@16:40:38.28 <closeIntake> Intake.close
@16:40:38.35 <closeExhaust> Exhaust.close
@16:40:38.53 <FILTERING> Toroid.in :hold
@16:40:38.59 Toroid.seek :bypass
@16:40:40.21 Toroid.in :default
@16:40:40.28 Toroid.seek :clear
@16:40:41.89 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@16:40:41.90 Pressure.stop
@16:40:42.51 Sampled  361.4ml
@16:40:44.17 <LRAUV> -> Cmd.startProcessing
@16:40:45.62 <PROCESSING> SPR.command \"read_sequences\"
@16:40:46.02 SPR.command \"stats_on\"
@16:40:46.14 SPR.command \"start 0 1\"
@16:40:46.87 <SPRlogger> SPR.start /var/log/esp/spr22jun0216h.spr.bz2
@16:40:49.35 <PROCESSING> Handoff.select :waste
@16:40:56.04 Syringe tare is 19% of full scale
@16:40:56.07 PinPort.in :locking
@16:40:56.15 PinPort.seek :locked
@16:40:56.62 <posArchive> Archive.in :fast
@16:40:56.67 <posLyS2> Lysis2.in :fast
@16:40:56.79 <PROCESSING> Lysis1.seek 1350uL
@16:40:56.82 <posArchive> Archive.seek :full
@16:40:56.91 <posLyS2> Lysis2.seek :full
@16:40:58.93 <PROCESSING> Plunge.seek :reagent
@16:40:59.83 Evacuating puck with filter bubble point of 50psi
@16:40:59.85 PinPort.seek :locked
@16:40:59.95 Handoff.select :waste
@16:41:06.53 Air syringe pumping up to 1 stroke to reach 8psi
@16:41:06.54 Air stroke #1 of 1
@16:41:06.63 Air.in :pressurize
@16:41:06.70 Air.seek :pressurized
@16:41:18.27 0.5psi after pressurize
@16:41:18.29 Air.in :compress
@16:41:18.37 Air.seek :evac
@16:41:22.48 0.9psi after compress
@16:41:35.05 0.1psi after compress (stable)
@16:41:35.07 Air.in :default
@16:41:35.14 Air.hold
@16:41:35.30 at 0.0psi Opened to waste
@16:41:35.31 Handoff.select :blocked
@16:41:35.45 Archive syringe pumping up to 8 strokes to reach 50psi
@16:41:35.46 Archive stroke #1 of 8
@16:41:35.55 Archive.in :pressurize
@16:41:35.61 Archive.seek :pressurized
@16:41:48.51 9.1psi after pressurize
@16:41:48.53 Archive.in :compress
@16:41:48.60 Archive.seek :empty
@16:42:03.29 28.5psi after compress
@16:42:17.86 33.6psi after compress (stable)
@16:42:17.88 Archive.in :gulp
@16:42:17.96 Archive.seek :topVent
@16:42:25.12 33.8psi after gulp
@16:42:37.69 33.7psi after gulp (stable)
@16:42:37.70 Archive stroke #2 of 8
@16:42:37.79 Archive.in :pressurize
@16:42:37.85 Archive.seek :pressurized
@16:42:50.71 33.6psi after pressurize
@16:42:50.73 Archive.in :compress
@16:42:50.85 Archive.seek :empty
@16:43:04.75 Reached 50.2 of 50.0psi after pumping 3443uL (1.99 strokes)
@16:43:04.76 Archive.in :default
@16:43:04.83 Archive.hold
@16:43:04.90 Handoff.select :waste
@16:43:13.09 delay 1:00
@16:44:13.12 Handoff.select :blocked
@16:44:35.82 Handoff.select :waste
@16:44:35.93 2.2psi at evacEnd
@16:44:35.94 Handoff.select :blocked
@16:44:36.07 Archive.in :fast
@16:44:36.14 Archive.seek :topVent
@16:44:49.73 Stable @2.9psi after Handoff blocked
@16:44:49.75 Archive syringe pumping up to 8 strokes to reach 33psi
@16:44:49.77 Archive stroke #1 of 8
@16:44:49.91 Archive.in :pressurize
@16:44:49.98 Archive.seek :pressurized
@16:45:02.87 4.6psi after pressurize
@16:45:02.88 Archive.in :compress
@16:45:02.96 Archive.seek :empty
@16:45:17.64 8.1psi after compress
@16:46:00.21 32.4psi after compress (stable)
@16:46:00.23 Archive.in :gulp
@16:46:00.31 Archive.seek :topVent
@16:46:01.30 Reached 33.0 of 32.9psi after pumping 1730uL (1.00 strokes)
@16:46:01.31 Archive.in :default
@16:46:01.38 Archive.hold
@16:46:02.12 Handoff.select :waste
@16:46:14.85 delay 1:00
@16:47:14.86 Handoff.select :blocked
@16:47:37.55 Handoff.select :waste
@16:47:38.05 2.5psi at evacEnd
@16:47:38.07 Air.seek :fullVent
@16:47:39.49 Archive.in :fast
@16:47:39.55 Archive.seek :topVent
@16:47:45.95 Seawater evacuated, Presure above/below filter released. Lysis begins
@16:47:46.04 Lysis1.in :slow
@16:47:46.11 Lysis1.seek 1050uL
@16:48:16.12 delay 5 seconds
@16:48:21.14 Handoff.select :blocked
@16:48:21.31 Lysis1.seek 450uL
@16:49:21.26 delay 3 seconds
@16:49:24.33 Air.in mix with 1.40048 parts Lysis1
@16:49:24.76 <Airmixer> Air.seek :evac
@16:49:24.81 <PROCESSING> Lysis1.seek 300uL
@16:49:39.99 Lysis1.seek :empty
@16:50:10.07 Lysis buffer delivered @27.2psi
@16:50:10.08 Polling Can every 2:00
@16:50:10.63 PuckHeater.configure :default #ambient 16.4C
@16:50:10.68 PuckHeater.seek 80C
@16:51:07.39 PuckHeater.hold 2:00,80C
@16:53:07.06 Polling Can every 2:00
@16:53:07.61 PuckHeater.configure :default #ambient 16.5C
@16:53:07.67 PuckHeater.hold 30 seconds,79C
@16:53:37.74 Polling Can every 2:00
@16:53:38.30 PuckHeater.configure :default #ambient 16.5C
@16:53:38.38 PuckHeater.hold 30 seconds,78.5C
@16:54:08.46 Polling Can every 2:00
@16:54:09.00 PuckHeater.configure :default #ambient 16.5C
@16:54:09.05 PuckHeater.hold 10:00,77.9C
@17:04:06.92 Lysis complete. Syringe pressure @37.1psi
@17:04:06.93 Handoff.select :blocked
@17:04:06.98 Archive syringe pumping up to 5 strokes to reach 40psi
@17:04:06.99 Archive stroke #1 of 5
@17:04:07.08 Archive.in :pressurize
@17:04:07.15 Archive.seek :pressurized
@17:04:20.01 36.9psi after pressurize
@17:04:20.03 Archive.in :compress
@17:04:20.10 Archive.seek :empty
@17:04:28.68 Reached 41.4 of 40.0psi after pumping 1599uL (0.92 strokes)
@17:04:28.69 Archive.in :default
@17:04:28.76 Archive.hold
@17:04:28.83 delay 2 seconds
@17:04:30.85 Archive.in :fast
@17:04:30.94 Archive.seek :full
@17:04:37.95 Lysis1.seek 138uL
@17:04:51.82 delay 1 second
@17:04:52.85 <fillArchive> Archive.seek :full
@17:04:52.89 <PROCESSING> Lysis1.seek :empty
@17:05:06.88 Initial air pressure @55.2psi
@17:05:06.96 Lysis1.in :extract
@17:05:07.03 Lysis1.seek 600uL,nil
@17:05:10.51 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@17:05:10.52 Archive stroke #1 of 6
@17:05:10.62 Archive.in :pressurize
@17:05:10.72 Archive.seek :pressurized
@17:05:23.61 15.9psi after pressurize
@17:05:23.63 Archive.in :compress
@17:05:23.70 Archive.seek :empty
@17:05:36.74 Archive.in :default
@17:05:36.79 Archive.hold
@17:05:36.95 <PROCESSING> Initial 600uL lysate @18.5psi
@17:05:36.96 Handoff.select :blocked
@17:05:37.06 Initial air pressure @19.8psi
@17:05:37.14 Lysis1.seek 825uL,nil
@17:05:38.41 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@17:05:38.42 Archive stroke #1 of 6
@17:05:38.57 Archive.in :compress
@17:05:38.63 Archive.seek :empty
@17:05:40.28 15.9psi after compress
@17:05:43.80 Archive.in :default
@17:05:43.85 Archive.hold
@17:05:44.04 <PROCESSING> Recovered 825uL lysate @16.5psi
@17:05:44.06 Air.in :halfSpeed
@17:05:44.13 Air.seek :fullVent
@17:05:46.34 Puck Top Vented, bottom @0.4psi
@17:05:46.35 Handoff.select :waste
@17:05:46.47 delay 5 seconds
@17:05:51.53 Handoff open to waste, bottom @-0.2psi
@17:05:51.55 Air.in :default
@17:05:51.62 Air.seek :evac
@17:05:53.04 Lysis1.in :DAmix
@17:05:53.15 Lysis2.in :DAmix
@17:05:53.56 <Lysis1mixer> Lysis1.seek 575uL
@17:05:53.60 <PROCESSING> Lysis2.seek 1480uL
@17:06:28.65 delay 5 seconds
@17:06:33.67 Handoff.select SPR
@17:06:33.79 SPR.command \"start 2 14\"
@17:06:34.35 SPR stabilized
@17:06:34.49 delay 10 seconds
@17:06:44.50 SPR.command \"start 1 0\"
@17:06:47.71 Waiting up to 4:00 for SPR to enter state 1
@17:08:50.09 SPR.command \"hold_on\"
@17:08:51.50 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@17:08:51.97 <Lysis1mixer> Lysis1.seek 275uL
@17:08:52.01 <PROCESSING> Lysis2.seek 1180uL
@17:09:34.05 delay 15 seconds
@17:09:49.07 SPR.command \"hold_off\"
@17:09:52.15 Waiting up to 38:00 for SPR to enter state 7
@17:30:41.98 <SPRlogger> SPRsummary:-52.34,169.331,170.605,220.453 after 3139s
@17:37:19.21 <PROCESSING> SPR analysis completed
@17:37:24.32 <SPRlogger> Ended log spr22jun0216h.spr.bz2
@17:37:24.33 <PROCESSING> SPR.command \"stats_off\"
@17:37:25.97 <LRAUV> -> Cmd.stop
@17:37:26.07 <closeHandoff> Handoff.select :close
@17:37:26.20 <LRAUV> Gate.power :heat,:OFF
@17:37:26.21 Gate.power :cartridge,:OFF
@17:37:26.30 Carousel.seek 29
@17:37:26.47 Plunge.seek :locked
@17:37:27.33 PinPort.in :unlocking
@17:37:27.39 PinPort.seek :clear
@17:37:27.94 <Archive> Archive.in :unlocking
@17:37:28.07 Archive.seek :clear
@17:37:28.18 <Air> Air.in :unlocking
@17:37:28.27 Air.seek :clear
@17:37:28.38 <Lysis1> Lysis1.in :unlocking
@17:37:28.47 Lysis1.seek :clear
@17:37:28.51 <Lysis2> Lysis2.in :unlocking
@17:37:28.62 Lysis2.seek :clear
@17:37:32.19 <LRAUV> Gate.power :motor,:OFF
@17:37:32.20 Gate.power :core,:OFF
@17:37:32.23 Gate.power :analytic1,:OFF
@17:37:32.25 Gate.power :analytic2,:OFF
@17:37:32.27 Gate.power :analytic3,:OFF
@17:37:32.30 Gate.power :main,:OFF
@17:37:32.32 Safely stopped and ready to power off
##-- file closed 2022-06-04T05:14:13.910Z