##-- file opened 2022-05-30T00:59:53.025Z
@17:59:54.10 -> showStatus
@17:59:56.14 -> Cmd.loadCartridge -6
@17:59:56.21 Gate.power :main,:ON,limit to 5000mA
@17:59:56.23 Gate.power :analytic1,:ON,limit to 5000mA
@17:59:56.25 Gate.power :analytic2,:ON,limit to 5000mA
@17:59:56.28 Gate.power :analytic3,:ON,limit to 5000mA
@17:59:56.42 Powering core bus
@17:59:58.68 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@17:59:59.19 <LRAUV> Archive.configure :default
@17:59:59.32 Air.configure :default
@17:59:59.42 Lysis1.configure :default
@17:59:59.63 Lysis2.configure :default
@17:59:59.88 Plunge.configure :default
@18:00:00.15 SamplePump.configure :default
@18:00:00.45 Toroid.configure :default
@18:00:00.73 Carousel.configure :default
@18:00:01.03 Pressure.configure :default
@18:00:01.16 ExtraPump.configure :default
@18:00:01.27 PinPort.configure :default
@18:00:01.33 All elf controllers running firmware version 4.79
@18:00:01.35 Gate.power :motor,:ON,limit to 5000mA
@18:00:01.54 <Archive_home> Archive.in :default
@18:00:01.83 Archive.home.jog 0
@18:00:02.00 <Toroid_home> Toroid.in :default
@18:00:02.08 <Plunge_home> Plunge.in :default
@18:00:02.12 <Lysis1_home> Lysis1.in :default
@18:00:02.18 <PinPort_home> PinPort.in :default
@18:00:02.24 <Air_home> Air.in :default
@18:00:02.30 <Lysis2_home> Lysis2.in :default
@18:00:02.48 <Toroid_home> Toroid.home.jog 0
@18:00:02.52 <Lysis1_home> Lysis1.home.jog 0
@18:00:02.56 <Plunge_home> Plunge.home.jog 0
@18:00:02.60 <PinPort_home> PinPort.home.jog 0
@18:00:02.62 <Air_home> Air.home.jog 0
@18:00:02.70 <Lysis2_home> Lysis2.home.jog 0
@18:00:03.07 <Archive_home> Archive.in :homing
@18:00:03.27 <Plunge_home> Plunge.in :homing
@18:00:03.37 <Archive_home> Archive.jog 1392
@18:00:03.39 <PinPort_home> PinPort.in :homing
@18:00:03.42 <Toroid_home> Toroid.in :homing
@18:00:03.45 <Lysis1_home> Lysis1.in :homing
@18:00:03.62 <Plunge_home> Plunge.jog 1549
@18:00:03.66 <PinPort_home> PinPort.jog -107
@18:00:03.69 <Toroid_home> Toroid.jog 633
@18:00:03.73 <Lysis1_home> Lysis1.jog 1379
@18:00:03.92 <Lysis2_home> Lysis2.in :homing
@18:00:03.93 <Air_home> Air.in :homing
@18:00:04.05 <Lysis2_home> Lysis2.jog 1393
@18:00:04.08 <Air_home> Air.jog 1391
@18:00:04.19 <PinPort_home> PinPort.setRawPosition! 0
@18:00:04.23 PinPort.seek :home
@18:00:04.32 PinPort.in :default
@18:00:04.66 <Archive_home> Archive.setRawPosition! 50
@18:00:04.70 Archive.seek :home
@18:00:04.97 <Plunge_home> Plunge.setRawPosition! 50
@18:00:05.02 Plunge.seek :home
@18:00:05.11 <Lysis1_home> Lysis1.setRawPosition! 50
@18:00:05.13 <Archive_home> Archive.in :default
@18:00:05.21 <Lysis1_home> Lysis1.seek :home
@18:00:05.37 <Plunge_home> Plunge.in :default
@18:00:05.39 <Lysis2_home> Lysis2.setRawPosition! 50
@18:00:05.42 <Air_home> Air.setRawPosition! 50
@18:00:05.53 <Lysis2_home> Lysis2.seek :home
@18:00:05.60 <Air_home> Air.seek :home
@18:00:05.74 <Lysis1_home> Lysis1.in :default
@18:00:05.91 <Lysis2_home> Lysis2.in :default
@18:00:06.00 <Toroid_home> Toroid.setRawPosition! 0
@18:00:06.01 <Air_home> Air.in :default
@18:00:06.09 <Toroid_home> Toroid.seek :home
@18:00:06.20 Toroid.in :default
@18:00:06.43 <LRAUV> Toroid.seek :clear
@18:00:08.05 Carousel.in :default
@18:00:08.11 Carousel.home.jog 0
@18:00:08.59 Carousel.in :default
@18:00:08.65 Carousel.seek 37
@18:00:08.83 Selecting Cartridge 36
@18:00:09.09 Carousel.seek 36
@18:00:09.57 Lysis1.in :fast
@18:00:09.63 Lysis1.seek :locked
@18:00:12.22 <Plunge> Plunge.seek :locked
@18:00:12.35 <Air> Air.in :fast
@18:00:12.41 Air.seek :locked
@18:00:14.88 <LRAUV> Powering cartridge bus
@18:00:17.87 Gate.setIR 1750,5000,0,6
@18:00:17.99 Gate.setIR 0,5000,0,6
@18:00:18.04 Gate.power :cartridge,:OFF
@18:00:18.57 Mounted archive Cartridge
@18:00:35.71 -> Cmd.startFiltering
@18:00:35.96 <FILTERING> Preparing to filter cartridge 36
@18:00:36.22 Priming sample loop w/100ml, bypass w/10ml
@18:00:36.24 SamplePump.setPosition! 0ml
@18:00:36.29 Bypass.open
@18:00:37.73 Intake.open
@18:00:38.48 Sea 49.5m deep (87.8psia)
@18:00:38.50 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@18:00:41.08 Vacuum tare is 54% of full scale
@18:00:41.21 <closeIntake> Intake.close
@18:00:41.27 <closeBypass> Bypass.close
@18:00:41.96 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 87.8psia
@18:00:41.98 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@18:00:46.55 Bypass.open
@18:00:47.22 Intake.open
@18:00:48.63 Pressure.in :default
@18:00:48.69 Pressure.hold
@18:00:48.87 SamplePump.in :prime
@18:00:48.94 SamplePump.seek 10ml,59 seconds
@18:00:57.56 Pressure.stop
@18:00:57.61 SamplePump.setPosition! 0ml
@18:00:57.67 Exhaust.open
@18:00:59.16 <closeBypass> Bypass.close
@18:01:00.66 <FILTERING> Pressure.in :default
@18:01:00.72 Pressure.hold
@18:01:00.83 Sea 50.0m deep (88.5psia)
@18:01:00.97 SamplePump.in :prime
@18:01:01.04 SamplePump.seek 100ml,5:16
@18:02:36.47 Pressure.stop
@18:02:36.53 Bypass.open
@18:02:37.20 Intake.close
@18:02:37.99 Toroid.in :hold
@18:02:38.05 Toroid.seek :crack
@18:02:39.55 Toroid.seek :cartridge
@18:02:40.63 Toroid.in :default
@18:02:40.70 Toroid.seek :clear
@18:02:42.66 <closeExhaust> Exhaust.close
@18:02:42.70 <closeBypass> Bypass.close
@18:02:43.47 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 88.0psia
@18:02:43.49 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@18:02:48.11 Sampling 1000.0ml
@18:02:48.13 SamplePump.setPosition! 0ml
@18:02:48.23 Exhaust.open
@18:02:49.01 Intake.open
@18:02:49.84 Pressure.in :default
@18:02:49.90 Pressure.hold
@18:02:50.01 Sea 49.6m deep (88.0psia)
@18:02:50.15 SamplePump.in :sample
@18:02:50.22 SamplePump.seek 1000ml,1:23:48
@18:02:52.88 <sampling> Cartridge 36 filtering 1/1000ml at 0.41ml/s with 24.8psi vacuum 50.8m deep (89.7psia)
@18:03:52.87 Cartridge 36 filtering 26/1000ml at 0.40ml/s with 26.2psi vacuum 51.3m deep (90.5psia)
@18:04:52.88 Cartridge 36 filtering 50/1000ml at 0.42ml/s with 22.8psi vacuum 48.5m deep (86.3psia)
@18:05:52.87 Cartridge 36 filtering 73/1000ml at 0.41ml/s with 21.2psi vacuum 51.7m deep (91.0psia)
@18:06:52.88 Cartridge 36 filtering 97/1000ml at 0.41ml/s with 22.9psi vacuum 49.3m deep (87.5psia)
@18:07:52.87 Cartridge 36 filtering 120/1000ml at 0.40ml/s with 26.5psi vacuum 48.9m deep (87.0psia)
@18:08:52.88 Cartridge 36 filtering 142/1000ml at 0.39ml/s with 22.3psi vacuum 50.5m deep (89.3psia)
@18:09:52.87 Cartridge 36 filtering 165/1000ml at 0.35ml/s with 24.7psi vacuum 47.5m deep (84.9psia)
@18:10:52.88 Cartridge 36 filtering 187/1000ml at 0.38ml/s with 26.5psi vacuum 47.5m deep (84.9psia)
@18:11:52.87 Cartridge 36 filtering 209/1000ml at 0.38ml/s with 21.8psi vacuum 51.0m deep (90.1psia)
@18:12:52.87 Cartridge 36 filtering 231/1000ml at 0.35ml/s with 21.0psi vacuum 49.4m deep (87.7psia)
@18:13:52.87 Cartridge 36 filtering 252/1000ml at 0.34ml/s with 24.1psi vacuum 45.8m deep (82.4psia)
@18:14:52.88 Cartridge 36 filtering 274/1000ml at 0.37ml/s with 27.1psi vacuum 46.0m deep (82.7psia)
@18:15:52.87 Cartridge 36 filtering 295/1000ml at 0.37ml/s with 27.0psi vacuum 46.7m deep (83.7psia)
@18:16:52.88 Cartridge 36 filtering 316/1000ml at 0.34ml/s with 21.1psi vacuum 51.3m deep (90.5psia)
@18:17:52.88 Cartridge 36 filtering 338/1000ml at 0.37ml/s with 22.1psi vacuum 48.9m deep (87.0psia)
@18:18:52.88 Cartridge 36 filtering 358/1000ml at 0.34ml/s with 20.7psi vacuum 46.3m deep (83.1psia)
@18:19:52.87 Cartridge 36 filtering 379/1000ml at 0.35ml/s with 26.0psi vacuum 47.4m deep (84.7psia)
@18:20:52.88 Cartridge 36 filtering 400/1000ml at 0.34ml/s with 23.1psi vacuum 49.6m deep (88.0psia)
@18:21:52.87 Cartridge 36 filtering 421/1000ml at 0.36ml/s with 22.7psi vacuum 46.5m deep (83.5psia)
@18:22:52.88 Cartridge 36 filtering 441/1000ml at 0.35ml/s with 23.2psi vacuum 49.7m deep (88.1psia)
@18:23:52.87 Cartridge 36 filtering 461/1000ml at 0.35ml/s with 27.5psi vacuum 46.3m deep (83.1psia)
@18:24:52.88 Cartridge 36 filtering 482/1000ml at 0.36ml/s with 23.5psi vacuum 47.0m deep (84.2psia)
@18:25:52.87 Cartridge 36 filtering 502/1000ml at 0.34ml/s with 27.3psi vacuum 46.0m deep (82.7psia)
@18:26:52.92 Cartridge 36 filtering 522/1000ml at 0.33ml/s with 24.4psi vacuum 48.5m deep (86.4psia)
@18:27:52.87 Cartridge 36 filtering 542/1000ml at 0.35ml/s with 27.1psi vacuum 45.8m deep (82.4psia)
@18:28:52.88 Cartridge 36 filtering 562/1000ml at 0.31ml/s with 24.5psi vacuum 47.6m deep (85.0psia)
@18:29:52.87 Cartridge 36 filtering 581/1000ml at 0.32ml/s with 20.9psi vacuum 50.7m deep (89.6psia)
@18:30:52.88 Cartridge 36 filtering 601/1000ml at 0.32ml/s with 25.2psi vacuum 44.9m deep (81.1psia)
@18:31:52.87 Cartridge 36 filtering 621/1000ml at 0.30ml/s with 24.9psi vacuum 44.6m deep (80.6psia)
@18:32:52.88 Cartridge 36 filtering 640/1000ml at 0.34ml/s with 23.4psi vacuum 47.6m deep (85.1psia)
@18:33:52.87 Cartridge 36 filtering 660/1000ml at 0.32ml/s with 25.1psi vacuum 46.8m deep (83.9psia)
@18:34:52.87 Cartridge 36 filtering 679/1000ml at 0.31ml/s with 25.9psi vacuum 46.0m deep (82.7psia)
@18:35:52.87 Cartridge 36 filtering 698/1000ml at 0.32ml/s with 24.1psi vacuum 45.8m deep (82.4psia)
@18:36:52.87 Cartridge 36 filtering 718/1000ml at 0.34ml/s with 22.4psi vacuum 48.3m deep (86.0psia)
@18:37:52.87 Cartridge 36 filtering 737/1000ml at 0.30ml/s with 24.5psi vacuum 44.3m deep (80.2psia)
@18:38:52.88 Cartridge 36 filtering 756/1000ml at 0.31ml/s with 21.1psi vacuum 49.3m deep (87.6psia)
@18:39:52.87 Cartridge 36 filtering 775/1000ml at 0.31ml/s with 21.4psi vacuum 49.4m deep (87.8psia)
@18:40:52.88 Cartridge 36 filtering 794/1000ml at 0.30ml/s with 23.0psi vacuum 49.0m deep (87.1psia)
@18:41:52.87 Cartridge 36 filtering 813/1000ml at 0.31ml/s with 25.5psi vacuum 46.4m deep (83.3psia)
@18:42:52.88 Cartridge 36 filtering 832/1000ml at 0.30ml/s with 21.7psi vacuum 47.0m deep (84.1psia)
@18:43:52.87 Cartridge 36 filtering 850/1000ml at 0.33ml/s with 23.6psi vacuum 47.0m deep (84.2psia)
@18:44:52.88 Cartridge 36 filtering 869/1000ml at 0.33ml/s with 24.0psi vacuum 46.4m deep (83.3psia)
@18:45:52.87 Cartridge 36 filtering 888/1000ml at 0.31ml/s with 23.8psi vacuum 46.4m deep (83.3psia)
@18:46:52.88 Cartridge 36 filtering 906/1000ml at 0.30ml/s with 23.3psi vacuum 47.4m deep (84.7psia)
@18:47:52.87 Cartridge 36 filtering 925/1000ml at 0.33ml/s with 25.5psi vacuum 48.2m deep (85.9psia)
@18:48:52.88 Cartridge 36 filtering 944/1000ml at 0.31ml/s with 23.2psi vacuum 49.2m deep (87.4psia)
@18:49:52.87 Cartridge 36 filtering 962/1000ml at 0.29ml/s with 24.2psi vacuum 44.8m deep (80.9psia)
@18:50:53.30 Cartridge 36 filtering 980/1000ml at 0.30ml/s with 25.6psi vacuum 44.8m deep (80.9psia)
@18:51:52.87 Cartridge 36 filtering 999/1000ml at 0.31ml/s with 23.3psi vacuum 45.8m deep (82.3psia)
@18:51:57.39 <FILTERING> Pressure.stop
@18:51:57.51 <closeIntake> Intake.close
@18:51:57.55 <closeExhaust> Exhaust.close
@18:51:57.72 <FILTERING> Toroid.in :hold
@18:51:57.79 Toroid.seek :bypass
@18:51:59.41 Toroid.in :default
@18:51:59.48 Toroid.seek :clear
@18:52:01.09 Sampled  1000.0ml
@18:52:02.46 <LRAUV> -> Cmd.startProcessing
@18:52:02.88 <PROCESSING> Plunge.seek :reagent
@18:52:03.67 Sample loop closed. Ready to Deliver Reagents
@18:52:03.69 Archive.seek :sealed
@18:52:07.63 Archive.seek :evac
@18:52:21.66 delay 1:00
@18:53:21.69 Archive.seek :clear
@18:53:39.16 RNAlater soak for 10:00
@19:03:39.18 Archive.seek :tVent
@19:03:41.61 delay 1 second
@19:03:42.63 Archive.seek :evac
@19:03:58.14 delay 10 seconds
@19:04:08.17 Sample preserved and evacuated
@19:04:09.31 <LRAUV> -> Cmd.stop
@19:04:09.46 Carousel.seek 36
@19:04:09.64 Plunge.seek :locked
@19:04:10.56 <Archive> Archive.in :unlocking
@19:04:10.69 Archive.seek :clear
@19:04:10.73 <Air> Air.in :unlocking
@19:04:10.89 Air.seek :clear
@19:04:10.93 <Lysis1> Lysis1.in :unlocking
@19:04:11.07 Lysis1.seek :clear
@19:04:14.85 <LRAUV> Gate.power :motor,:OFF
@19:04:14.87 Gate.power :core,:OFF
@19:04:14.89 Gate.power :analytic1,:OFF
@19:04:14.91 Gate.power :analytic2,:OFF
@19:04:14.94 Gate.power :analytic3,:OFF
@19:04:14.96 Gate.power :main,:OFF
@19:04:14.98 Safely stopped and ready to power off
##-- file closed 2022-05-30T02:17:22.118Z

##-- file opened 2022-05-30T02:17:22.120Z
@19:17:23.20 -> showStatus
@19:17:25.22 -> Cmd.loadCartridge -10
@19:17:25.29 Gate.power :main,:ON,limit to 5000mA
@19:17:25.30 Gate.power :analytic1,:ON,limit to 5000mA
@19:17:25.33 Gate.power :analytic2,:ON,limit to 5000mA
@19:17:25.35 Gate.power :analytic3,:ON,limit to 5000mA
@19:17:25.50 Powering core bus
@19:17:27.75 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@19:17:28.26 <LRAUV> Archive.configure :default
@19:17:28.53 Air.configure :default
@19:17:28.76 Lysis1.configure :default
@19:17:29.04 Lysis2.configure :default
@19:17:29.35 Plunge.configure :default
@19:17:29.65 SamplePump.configure :default
@19:17:29.84 Toroid.configure :default
@19:17:29.96 Carousel.configure :default
@19:17:30.09 Pressure.configure :default
@19:17:30.21 ExtraPump.configure :default
@19:17:30.32 PinPort.configure :default
@19:17:30.38 All elf controllers running firmware version 4.79
@19:17:30.40 Gate.power :motor,:ON,limit to 5000mA
@19:17:30.66 <Archive_home> Archive.in :default
@19:17:30.71 <Air_home> Air.in :default
@19:17:30.96 Air.home.jog 0
@19:17:30.99 <Archive_home> Archive.home.jog 0
@19:17:31.17 <Toroid_home> Toroid.in :default
@19:17:31.22 <Plunge_home> Plunge.in :default
@19:17:31.26 <Lysis1_home> Lysis1.in :default
@19:17:31.31 <PinPort_home> PinPort.in :default
@19:17:31.36 <Lysis2_home> Lysis2.in :default
@19:17:31.47 <Toroid_home> Toroid.home.jog 0
@19:17:31.55 <Lysis1_home> Lysis1.home.jog 0
@19:17:31.59 <PinPort_home> PinPort.home.jog 0
@19:17:31.60 <Plunge_home> Plunge.home.jog 0
@19:17:31.65 <Lysis2_home> Lysis2.home.jog 0
@19:17:32.34 <Toroid_home> Toroid.in :homing
@19:17:32.36 <Plunge_home> Plunge.in :homing
@19:17:32.40 <PinPort_home> PinPort.in :homing
@19:17:32.43 <Lysis1_home> Lysis1.in :homing
@19:17:32.46 <Air_home> Air.in :homing
@19:17:32.50 <Archive_home> Archive.in :homing
@19:17:32.54 <Lysis2_home> Lysis2.in :homing
@19:17:32.77 <Plunge_home> Plunge.jog 1558
@19:17:32.81 <PinPort_home> PinPort.jog -107
@19:17:32.83 <Lysis1_home> Lysis1.jog 1379
@19:17:32.86 <Toroid_home> Toroid.jog 630
@19:17:32.89 <Air_home> Air.jog 1392
@19:17:32.91 <Lysis2_home> Lysis2.jog 1396
@19:17:32.98 <Archive_home> Archive.jog 1386
@19:17:33.24 <PinPort_home> PinPort.setRawPosition! 0
@19:17:33.28 PinPort.seek :home
@19:17:33.37 PinPort.in :default
@19:17:34.14 <Plunge_home> Plunge.setRawPosition! 50
@19:17:34.21 Plunge.seek :home
@19:17:34.32 <Lysis1_home> Lysis1.setRawPosition! 50
@19:17:34.35 <Lysis2_home> Lysis2.setRawPosition! 50
@19:17:34.39 <Archive_home> Archive.setRawPosition! 50
@19:17:34.42 <Air_home> Air.setRawPosition! 50
@19:17:34.51 <Lysis1_home> Lysis1.seek :home
@19:17:34.58 <Lysis2_home> Lysis2.seek :home
@19:17:34.63 <Archive_home> Archive.seek :home
@19:17:34.74 <Air_home> Air.seek :home
@19:17:34.91 <Plunge_home> Plunge.in :default
@19:17:34.93 <Lysis1_home> Lysis1.in :default
@19:17:35.09 <Lysis2_home> Lysis2.in :default
@19:17:35.12 <Archive_home> Archive.in :default
@19:17:35.25 <Toroid_home> Toroid.setRawPosition! 0
@19:17:35.28 <Air_home> Air.in :default
@19:17:35.36 <Toroid_home> Toroid.seek :home
@19:17:35.47 Toroid.in :default
@19:17:35.70 <LRAUV> Toroid.seek :clear
@19:17:37.32 Carousel.in :default
@19:17:37.38 Carousel.home.jog 0
@19:17:37.87 Carousel.in :default
@19:17:37.93 Carousel.seek 36
@19:17:38.08 Selecting Cartridge 35
@19:17:38.34 Carousel.seek 35
@19:17:38.82 Lysis1.in :fast
@19:17:38.89 Lysis1.seek :locked
@19:17:41.48 <Plunge> Plunge.seek :locked
@19:17:41.62 <Air> Air.in :fast
@19:17:41.68 Air.seek :locked
@19:17:44.54 <LRAUV> Powering cartridge bus
@19:17:47.14 Gate.setIR 1750,5000,0,6
@19:17:47.30 Gate.setIR 0,5000,0,6
@19:17:47.37 PuckHeater.configure :default
@19:17:47.43 Gate.power :heat,:ON,limit to 5000mA
@19:17:48.09 Mounted lysis Cartridge board#Q05046
@19:17:48.13 Using default puck heat loss curve for HeatingAccelInCarousel
@19:18:05.62 -> Cmd.startFiltering
@19:18:05.86 <FILTERING> Preparing to filter cartridge 35
@19:18:06.13 Priming sample loop w/100ml, bypass w/10ml
@19:18:06.14 SamplePump.setPosition! 0ml
@19:18:06.20 Bypass.open
@19:18:07.65 Intake.open
@19:18:08.37 Sea 50.3m deep (89.1psia)
@19:18:08.39 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@19:18:10.96 Vacuum tare is 54% of full scale
@19:18:11.10 <closeIntake> Intake.close
@19:18:11.15 <closeBypass> Bypass.close
@19:18:11.89 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 89.1psia
@19:18:11.91 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@19:18:16.48 Toroid pressure rose 1% to 89.6psia
@19:18:16.49 Bypass.open
@19:18:17.18 Intake.open
@19:18:18.64 Pressure.in :default
@19:18:18.70 Pressure.hold
@19:18:18.88 SamplePump.in :prime
@19:18:18.95 SamplePump.seek 10ml,59 seconds
@19:18:27.58 Pressure.stop
@19:18:27.63 SamplePump.setPosition! 0ml
@19:18:27.68 Exhaust.open
@19:18:29.17 <closeBypass> Bypass.close
@19:18:30.68 <FILTERING> Pressure.in :default
@19:18:30.75 Pressure.hold
@19:18:30.86 Sea 50.2m deep (88.9psia)
@19:18:31.00 SamplePump.in :prime
@19:18:31.07 SamplePump.seek 100ml,5:16
@19:19:39.57 SamplePump.seek 100ml,1:52
@19:20:07.04 Pressure.stop
@19:20:07.10 Bypass.open
@19:20:07.80 Intake.close
@19:20:08.59 Toroid.in :hold
@19:20:08.65 Toroid.seek :crack
@19:20:10.15 Toroid.seek :cartridge
@19:20:11.24 Toroid.in :default
@19:20:11.30 Toroid.seek :clear
@19:20:13.27 <closeExhaust> Exhaust.close
@19:20:13.32 <closeBypass> Bypass.close
@19:20:14.11 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 90.0psia
@19:20:14.13 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@19:20:18.75 Sampling 1000.0ml
@19:20:18.76 SamplePump.setPosition! 0ml
@19:20:18.82 Exhaust.open
@19:20:19.60 Intake.open
@19:20:20.41 Pressure.in :default
@19:20:20.47 Pressure.hold
@19:20:20.59 Sea 51.2m deep (90.3psia)
@19:20:20.73 SamplePump.in :sample
@19:20:20.80 SamplePump.seek 1000ml,1:23:48
@19:20:23.44 <sampling> Cartridge 35 filtering 1/1000ml at 0.43ml/s with 22.8psi vacuum 52.5m deep (92.4psia)
@19:21:23.44 Cartridge 35 filtering 26/1000ml at 0.41ml/s with 21.0psi vacuum 50.3m deep (89.1psia)
@19:22:23.44 Cartridge 35 filtering 51/1000ml at 0.40ml/s with 22.6psi vacuum 49.6m deep (88.0psia)
@19:23:23.44 Cartridge 35 filtering 76/1000ml at 0.42ml/s with 25.6psi vacuum 51.3m deep (90.6psia)
@19:24:23.44 Cartridge 35 filtering 102/1000ml at 0.41ml/s with 20.8psi vacuum 49.3m deep (87.6psia)
@19:25:23.44 Cartridge 35 filtering 126/1000ml at 0.42ml/s with 19.1psi vacuum 51.7m deep (91.1psia)
@19:26:23.44 Cartridge 35 filtering 151/1000ml at 0.40ml/s with 27.8psi vacuum 48.2m deep (85.9psia)
@19:27:23.44 Cartridge 35 filtering 176/1000ml at 0.40ml/s with 24.3psi vacuum 48.5m deep (86.4psia)
@19:28:23.44 Cartridge 35 filtering 201/1000ml at 0.39ml/s with 22.3psi vacuum 52.3m deep (92.0psia)
@19:29:23.44 Cartridge 35 filtering 225/1000ml at 0.42ml/s with 25.6psi vacuum 47.8m deep (85.3psia)
@19:30:23.45 Cartridge 35 filtering 249/1000ml at 0.41ml/s with 26.2psi vacuum 47.9m deep (85.5psia)
@19:31:23.44 Cartridge 35 filtering 274/1000ml at 0.41ml/s with 24.6psi vacuum 49.8m deep (88.2psia)
@19:32:23.44 Cartridge 35 filtering 298/1000ml at 0.42ml/s with 22.9psi vacuum 48.7m deep (86.7psia)
@19:33:23.44 Cartridge 35 filtering 322/1000ml at 0.39ml/s with 27.3psi vacuum 48.9m deep (87.0psia)
@19:34:23.44 Cartridge 35 filtering 346/1000ml at 0.40ml/s with 21.8psi vacuum 48.5m deep (86.3psia)
@19:35:23.45 Cartridge 35 filtering 370/1000ml at 0.42ml/s with 23.7psi vacuum 51.5m deep (90.9psia)
@19:36:23.44 Cartridge 35 filtering 394/1000ml at 0.39ml/s with 23.5psi vacuum 49.0m deep (87.1psia)
@19:37:23.44 Cartridge 35 filtering 418/1000ml at 0.38ml/s with 28.6psi vacuum 46.1m deep (82.9psia)
@19:38:23.44 Cartridge 35 filtering 442/1000ml at 0.39ml/s with 21.1psi vacuum 48.3m deep (86.0psia)
@19:39:23.44 Cartridge 35 filtering 465/1000ml at 0.40ml/s with 19.2psi vacuum 52.6m deep (92.5psia)
@19:40:23.44 Cartridge 35 filtering 489/1000ml at 0.41ml/s with 23.1psi vacuum 48.5m deep (86.3psia)
@19:41:23.44 Cartridge 35 filtering 512/1000ml at 0.37ml/s with 23.2psi vacuum 50.6m deep (89.5psia)
@19:42:23.44 Cartridge 35 filtering 536/1000ml at 0.40ml/s with 19.1psi vacuum 52.5m deep (92.3psia)
@19:43:23.44 Cartridge 35 filtering 559/1000ml at 0.41ml/s with 27.0psi vacuum 48.5m deep (86.4psia)
@19:44:23.45 Cartridge 35 filtering 582/1000ml at 0.41ml/s with 24.0psi vacuum 51.4m deep (90.7psia)
@19:45:23.44 Cartridge 35 filtering 606/1000ml at 0.41ml/s with 21.3psi vacuum 52.3m deep (91.9psia)
@19:46:23.44 Cartridge 35 filtering 629/1000ml at 0.39ml/s with 26.5psi vacuum 49.3m deep (87.6psia)
@19:47:23.44 Cartridge 35 filtering 651/1000ml at 0.35ml/s with 21.9psi vacuum 49.5m deep (87.8psia)
@19:48:23.44 Cartridge 35 filtering 673/1000ml at 0.36ml/s with 22.7psi vacuum 48.7m deep (86.7psia)
@19:49:23.44 Cartridge 35 filtering 695/1000ml at 0.36ml/s with 21.1psi vacuum 48.3m deep (86.0psia)
@19:50:23.44 Cartridge 35 filtering 717/1000ml at 0.34ml/s with 22.5psi vacuum 50.8m deep (89.8psia)
@19:51:23.44 Cartridge 35 filtering 739/1000ml at 0.38ml/s with 23.5psi vacuum 48.3m deep (86.1psia)
@19:52:23.44 Cartridge 35 filtering 761/1000ml at 0.35ml/s with 27.8psi vacuum 46.7m deep (83.8psia)
@19:53:23.44 Cartridge 35 filtering 783/1000ml at 0.34ml/s with 24.2psi vacuum 49.4m deep (87.7psia)
@19:54:23.44 Cartridge 35 filtering 804/1000ml at 0.34ml/s with 24.4psi vacuum 48.7m deep (86.7psia)
@19:55:23.44 Cartridge 35 filtering 826/1000ml at 0.37ml/s with 26.6psi vacuum 49.1m deep (87.3psia)
@19:56:23.44 Cartridge 35 filtering 847/1000ml at 0.34ml/s with 23.8psi vacuum 50.1m deep (88.8psia)
@19:57:23.44 Cartridge 35 filtering 869/1000ml at 0.34ml/s with 21.3psi vacuum 50.6m deep (89.5psia)
@19:58:23.44 Cartridge 35 filtering 890/1000ml at 0.34ml/s with 24.3psi vacuum 49.3m deep (87.6psia)
@19:59:23.44 Cartridge 35 filtering 911/1000ml at 0.35ml/s with 25.2psi vacuum 48.2m deep (85.9psia)
@20:00:23.49 Cartridge 35 filtering 932/1000ml at 0.35ml/s with 23.8psi vacuum 46.0m deep (82.7psia)
@20:01:23.44 Cartridge 35 filtering 953/1000ml at 0.36ml/s with 27.1psi vacuum 46.6m deep (83.5psia)
@20:02:23.44 Cartridge 35 filtering 974/1000ml at 0.36ml/s with 25.7psi vacuum 48.9m deep (87.0psia)
@20:03:23.88 Cartridge 35 filtering 995/1000ml at 0.36ml/s with 21.9psi vacuum 52.7m deep (92.6psia)
@20:03:36.54 <FILTERING> Pressure.stop
@20:03:36.66 <closeIntake> Intake.close
@20:03:36.70 <closeExhaust> Exhaust.close
@20:03:36.88 <FILTERING> Toroid.in :hold
@20:03:36.95 Toroid.seek :bypass
@20:03:38.56 Toroid.in :default
@20:03:38.62 Toroid.seek :clear
@20:03:40.23 Sampled  1000.0ml
@20:03:41.68 <LRAUV> -> Cmd.startProcessing
@20:03:43.11 <PROCESSING> SPR.command \"read_sequences\"
@20:03:43.64 SPR.command \"stats_on\"
@20:03:43.75 SPR.command \"start 0 1\"
@20:03:44.50 <SPRlogger> SPR.start /var/log/esp/spr22may2920h.spr.bz2
@20:03:46.49 <PROCESSING> Handoff.select :waste
@20:03:53.17 Syringe tare is 18% of full scale
@20:03:53.20 PinPort.in :locking
@20:03:53.27 PinPort.seek :locked
@20:03:53.76 <posArchive> Archive.in :fast
@20:03:53.80 <posLyS2> Lysis2.in :fast
@20:03:53.89 <PROCESSING> Lysis1.seek 1350uL
@20:03:53.96 <posArchive> Archive.seek :full
@20:03:54.03 <posLyS2> Lysis2.seek :full
@20:03:56.01 <PROCESSING> Plunge.seek :reagent
@20:03:56.88 Evacuating puck with filter bubble point of 50psi
@20:03:56.91 PinPort.seek :locked
@20:03:56.99 Handoff.select :waste
@20:04:04.07 Air syringe pumping up to 1 stroke to reach 8psi
@20:04:04.08 Air stroke #1 of 1
@20:04:04.18 Air.in :pressurize
@20:04:04.24 Air.seek :pressurized
@20:04:15.82 1.3psi after pressurize
@20:04:15.84 Air.in :compress
@20:04:15.91 Air.seek :evac
@20:04:20.06 1.3psi after compress
@20:04:32.64 -0.1psi after compress (stable)
@20:04:32.66 Air.in :default
@20:04:32.73 Air.hold
@20:04:32.90 at -0.1psi Opened to waste
@20:04:32.91 Handoff.select :blocked
@20:04:33.04 Archive syringe pumping up to 8 strokes to reach 50psi
@20:04:33.05 Archive stroke #1 of 8
@20:04:33.14 Archive.in :pressurize
@20:04:33.21 Archive.seek :pressurized
@20:04:46.06 0.0psi after pressurize
@20:04:46.08 Archive.in :compress
@20:04:46.16 Archive.seek :empty
@20:05:00.85 25.3psi after compress
@20:05:13.42 25.0psi after compress (stable)
@20:05:13.44 Archive.in :gulp
@20:05:13.52 Archive.seek :topVent
@20:05:20.68 25.4psi after gulp
@20:05:33.24 25.4psi after gulp (stable)
@20:05:33.25 Archive stroke #2 of 8
@20:05:33.35 Archive.in :pressurize
@20:05:33.41 Archive.seek :pressurized
@20:05:46.26 25.1psi after pressurize
@20:05:46.28 Archive.in :compress
@20:05:46.36 Archive.seek :empty
@20:06:01.04 26.9psi after compress
@20:06:13.62 27.9psi after compress (stable)
@20:06:13.64 Archive.in :gulp
@20:06:13.72 Archive.seek :topVent
@20:06:20.87 29.5psi after gulp
@20:06:33.47 30.3psi after gulp (stable)
@20:06:33.49 Archive stroke #3 of 8
@20:06:33.59 Archive.in :pressurize
@20:06:33.66 Archive.seek :pressurized
@20:06:46.92 31.1psi after pressurize
@20:06:46.94 Archive.in :compress
@20:06:47.02 Archive.seek :empty
@20:07:01.70 33.9psi after compress
@20:08:08.77 45.5psi after compress (stable)
@20:08:08.79 Archive.in :gulp
@20:08:08.87 Archive.seek :topVent
@20:08:16.44 48.0psi after gulp
@20:08:29.07 49.4psi after gulp (stable)
@20:08:29.09 Archive stroke #4 of 8
@20:08:29.18 Archive.in :pressurize
@20:08:29.25 Archive.seek :pressurized
@20:08:31.96 Reached 50.0 of 50.0psi after pumping 5462uL (3.16 strokes)
@20:08:31.98 Archive.in :default
@20:08:32.05 Archive.hold
@20:08:32.13 Handoff.select :waste
@20:08:45.31 delay 1:00
@20:09:45.33 Handoff.select :blocked
@20:10:08.00 Handoff.select :waste
@20:10:08.12 1.3psi at evacEnd
@20:10:08.15 Handoff.select :blocked
@20:10:08.29 Archive.in :fast
@20:10:08.35 Archive.seek :topVent
@20:10:16.94 Stable @0.6psi after Handoff blocked
@20:10:16.96 Archive syringe pumping up to 8 strokes to reach 31psi
@20:10:16.98 Archive stroke #1 of 8
@20:10:17.07 Archive.in :pressurize
@20:10:17.14 Archive.seek :pressurized
@20:10:30.00 1.4psi after pressurize
@20:10:30.03 Archive.in :compress
@20:10:30.10 Archive.seek :empty
@20:10:44.79 8.1psi after compress
@20:11:09.87 15.7psi after compress (stable)
@20:11:09.89 Archive.in :gulp
@20:11:09.96 Archive.seek :topVent
@20:11:17.12 16.7psi after gulp
@20:11:29.70 17.5psi after gulp (stable)
@20:11:29.71 Archive stroke #2 of 8
@20:11:29.80 Archive.in :pressurize
@20:11:29.87 Archive.seek :pressurized
@20:11:42.74 18.3psi after pressurize
@20:11:42.76 Archive.in :compress
@20:11:42.84 Archive.seek :empty
@20:11:57.52 23.4psi after compress
@20:12:15.64 Reached 30.6 of 30.6psi after pumping 3460uL (2.00 strokes)
@20:12:15.65 Archive.in :default
@20:12:15.72 Archive.hold
@20:12:15.79 Handoff.select :waste
@20:12:23.00 delay 1:00
@20:13:23.01 Handoff.select :blocked
@20:13:45.74 Handoff.select :waste
@20:13:45.85 2.1psi at evacEnd
@20:13:45.87 Air.seek :fullVent
@20:13:47.29 Archive.in :fast
@20:13:47.35 Archive.seek :topVent
@20:13:54.45 Seawater evacuated, Presure above/below filter released. Lysis begins
@20:13:54.54 Lysis1.in :slow
@20:13:54.61 Lysis1.seek 1050uL
@20:14:24.62 delay 5 seconds
@20:14:29.64 Handoff.select :blocked
@20:14:29.81 Lysis1.seek 450uL
@20:15:29.79 delay 3 seconds
@20:15:32.86 Air.in mix with 1.40048 parts Lysis1
@20:15:33.33 <Airmixer> Air.seek :evac
@20:15:33.37 <PROCESSING> Lysis1.seek 300uL
@20:15:48.49 Lysis1.seek :empty
@20:16:18.60 Lysis buffer delivered @41.7psi
@20:16:18.61 Polling Can every 2:00
@20:16:19.16 PuckHeater.configure :default #ambient 11.6C
@20:16:19.21 PuckHeater.seek 80.2C
@20:17:28.52 PuckHeater.hold 2:00,80.2C
@20:19:28.56 Polling Can every 2:00
@20:19:29.10 PuckHeater.configure :default #ambient 11.7C
@20:19:29.15 PuckHeater.hold 30 seconds,79.2C
@20:19:59.34 Polling Can every 2:00
@20:19:59.88 PuckHeater.configure :default #ambient 11.6C
@20:19:59.93 PuckHeater.hold 30 seconds,78.6C
@20:20:30.13 Polling Can every 2:00
@20:20:30.67 PuckHeater.configure :default #ambient 11.6C
@20:20:30.73 PuckHeater.hold 10:00,78.1C
@20:30:30.69 Lysis complete. Syringe pressure @58.3psi
@20:30:30.71 Handoff.select :blocked
@20:30:30.76 Archive syringe pumping up to 5 strokes to reach 40psi
@20:30:30.77 Archive stroke #1 of 5
@20:30:30.86 Archive.in :pressurize
@20:30:30.92 Archive.seek :pressurized
@20:30:31.12 Reached 58.4 of 40.0psi after pumping 0uL (0.00 strokes)
@20:30:31.13 Archive.in :default
@20:30:31.21 Archive.hold
@20:30:31.28 delay 2 seconds
@20:30:33.30 Archive.in :fast
@20:30:33.36 Archive.seek :full
@20:30:33.53 Lysis1.seek 138uL
@20:30:47.41 delay 1 second
@20:30:48.44 <fillArchive> Archive.seek :full
@20:30:48.47 <PROCESSING> Lysis1.seek :empty
@20:31:02.46 Initial air pressure @56.9psi
@20:31:02.54 Lysis1.in :extract
@20:31:02.63 Lysis1.seek 600uL,nil
@20:31:06.10 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@20:31:06.12 Archive stroke #1 of 6
@20:31:06.21 Archive.in :pressurize
@20:31:06.28 Archive.seek :pressurized
@20:31:19.14 13.3psi after pressurize
@20:31:19.16 Archive.in :compress
@20:31:19.26 Archive.seek :empty
@20:31:24.51 Reached 29.6 of 25.0psi after pumping 1526uL (0.88 strokes)
@20:31:24.52 Archive.in :default
@20:31:24.59 Archive.hold
@20:31:30.90 Archive syringe pumping up to 6 strokes to reach 25psi
@20:31:30.91 Archive stroke #1 of 6
@20:31:31.06 Archive.in :compress
@20:31:31.14 Archive.hold
@20:31:31.27 <PROCESSING> Initial 600uL lysate @17.1psi
@20:31:31.28 Handoff.select :blocked
@20:31:31.38 Initial air pressure @17.3psi
@20:31:31.45 Lysis1.seek 825uL,nil
@20:31:32.68 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@20:31:32.69 Archive stroke #1 of 6
@20:31:32.79 Archive.in :compress
@20:31:32.86 Archive.seek :empty
@20:31:36.95 Archive.in :default
@20:31:37.00 Archive.hold
@20:31:37.22 <PROCESSING> Recovered 825uL lysate @16.4psi
@20:31:37.24 Air.in :halfSpeed
@20:31:37.34 Air.seek :fullVent
@20:31:39.54 Puck Top Vented, bottom @0.2psi
@20:31:39.55 Handoff.select :waste
@20:31:39.68 delay 5 seconds
@20:31:44.74 Handoff open to waste, bottom @0.1psi
@20:31:44.76 Air.in :default
@20:31:44.83 Air.seek :evac
@20:31:46.27 Lysis2.in :DAmix
@20:31:46.38 Lysis1.in :DAmix
@20:31:46.78 <Lysis2mixer> Lysis2.seek 1480uL
@20:31:46.86 <PROCESSING> Lysis1.seek 575uL
@20:32:21.89 delay 5 seconds
@20:32:26.91 Handoff.select SPR
@20:32:27.03 SPR.command \"start 2 14\"
@20:32:27.91 SPR stabilized
@20:32:28.09 delay 10 seconds
@20:32:38.10 SPR.command \"start 1 0\"
@20:32:41.27 Waiting up to 4:00 for SPR to enter state 1
@20:34:43.64 SPR.command \"hold_on\"
@20:34:45.54 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@20:34:45.97 <Lysis2mixer> Lysis2.seek 1180uL
@20:34:46.01 <PROCESSING> Lysis1.seek 275uL
@20:35:28.04 delay 15 seconds
@20:35:43.07 SPR.command \"hold_off\"
@20:35:46.19 Waiting up to 38:00 for SPR to enter state 7
@20:56:35.54 <SPRlogger> SPRsummary:-33.204,119.645,139.325,180.228 after 3325s
@21:03:12.76 <PROCESSING> SPR analysis completed
@21:03:19.33 <SPRlogger> Ended log spr22may2920h.spr.bz2
@21:03:19.34 <PROCESSING> SPR.command \"stats_off\"
@21:03:20.80 <LRAUV> -> Cmd.stop
@21:03:20.90 <closeHandoff> Handoff.select :close
@21:03:21.03 <LRAUV> Gate.power :heat,:OFF
@21:03:21.05 Gate.power :cartridge,:OFF
@21:03:21.14 Carousel.seek 35
@21:03:21.32 Plunge.seek :locked
@21:03:22.18 PinPort.in :unlocking
@21:03:22.24 PinPort.seek :clear
@21:03:22.78 <Archive> Archive.in :unlocking
@21:03:22.90 Archive.seek :clear
@21:03:22.95 <Air> Air.in :unlocking
@21:03:23.11 Air.seek :clear
@21:03:23.15 <Lysis1> Lysis1.in :unlocking
@21:03:23.30 Lysis1.seek :clear
@21:03:23.40 <Lysis2> Lysis2.in :unlocking
@21:03:23.47 Lysis2.seek :clear
@21:03:27.02 <LRAUV> Gate.power :motor,:OFF
@21:03:27.04 Gate.power :core,:OFF
@21:03:27.06 Gate.power :analytic1,:OFF
@21:03:27.08 Gate.power :analytic2,:OFF
@21:03:27.11 Gate.power :analytic3,:OFF
@21:03:27.13 Gate.power :main,:OFF
@21:03:27.16 Safely stopped and ready to power off