##-- file opened 2019-08-20T12:07:45.547Z @08:07:46.49 -> showStatus @08:07:48.11 -> Cmd.loadCartridge -4 @08:07:48.26 Gate.power :main,:ON,limit to 5000mA @08:07:48.27 Gate.power :analytic1,:ON,limit to 5000mA @08:07:48.30 Gate.power :analytic2,:ON,limit to 5000mA @08:07:48.32 Gate.power :analytic3,:ON,limit to 5000mA @08:07:48.45 Powering core bus @08:07:50.73 SPR Module 1, FW Rev. B, FPGA Rev. C @08:07:50.86 Archive.configure :default @08:07:51.03 Air.configure :default @08:07:51.13 Lysis1.configure :default @08:07:51.23 Lysis2.configure :default @08:07:51.35 Plunge.configure :default @08:07:51.47 SamplePump.configure :default @08:07:51.59 ExtraPump.configure :default @08:07:51.71 Toroid.configure :default @08:07:51.83 Carousel.configure :default @08:07:51.95 Pressure.configure :default @08:07:52.06 PinPort.configure :default @08:07:52.12 All elf controllers running firmware version 4.58 @08:07:52.14 Gate.power :motor,:ON,limit to 5000mA @08:07:52.33 Archive.reconfigure :default @08:07:52.41 Air.reconfigure :default @08:07:52.68 Air.home.jog 0 @08:07:52.73 Archive.home.jog 0 @08:07:52.90 Toroid.reconfigure :default @08:07:52.94 Plunge.reconfigure :default @08:07:52.98 Lysis1.reconfigure :default @08:07:53.06 Lysis2.reconfigure :default @08:07:53.12 PinPort.reconfigure :default @08:07:53.32 Lysis2.home.jog 0 @08:07:53.37 Toroid.home.jog 0 @08:07:53.43 PinPort.home.jog 0 @08:07:53.49 Lysis1.home.jog 0 @08:07:53.55 Plunge.home.jog 0 @08:07:53.77 Archive.reconfigure :homing @08:07:53.88 Air.reconfigure :homing @08:07:54.00 Air.jog 1408 @08:07:54.05 Archive.jog 1379 @08:07:54.20 Toroid.reconfigure :homing @08:07:54.24 Plunge.reconfigure :homing @08:07:54.27 PinPort.reconfigure :homing @08:07:54.31 Lysis1.reconfigure :homing @08:07:54.35 Lysis2.reconfigure :homing @08:07:54.57 Lysis2.jog 1427 @08:07:54.62 Toroid.jog 639 @08:07:54.68 Plunge.jog 1548 @08:07:54.73 Lysis1.jog 1399 @08:07:54.79 PinPort.jog -110 @08:07:55.02 PinPort.setRawPosition! 0 @08:07:55.06 PinPort.seek :home @08:07:55.17 PinPort.reconfigure :default @08:07:55.20 Air.setRawPosition! 50 @08:07:55.26 Air.seek :home @08:07:55.36 Archive.setRawPosition! 50 @08:07:55.41 Archive.seek :home @08:07:55.57 Air.reconfigure :default @08:07:55.69 Archive.reconfigure :default @08:07:55.77 Lysis2.setRawPosition! 50 @08:07:55.83 Lysis2.seek :home @08:07:55.92 Plunge.setRawPosition! 50 @08:07:55.94 Lysis1.setRawPosition! 50 @08:07:56.00 Plunge.seek :home @08:07:56.05 Lysis1.seek :home @08:07:56.22 Lysis2.reconfigure :default @08:07:56.41 Plunge.reconfigure :default @08:07:56.43 Lysis1.reconfigure :default @08:07:56.79 Toroid.setRawPosition! 0 @08:07:56.83 Toroid.seek :home @08:07:56.92 Toroid.reconfigure :default @08:07:57.05 Toroid.seek :clear @08:07:58.67 Carousel.reconfigure :default @08:07:58.74 Carousel.home.jog 0 @08:07:59.23 Carousel.seek 44 @08:07:59.39 Selecting Cartridge 45 @08:07:59.63 Carousel.seek 45 @08:08:00.11 Lysis1.seek :locked @08:08:04.24 Plunge.seek :locked @08:08:04.36 Air.seek :locked @08:08:08.48 Powering cartridge bus @08:08:10.62 Gate.setIR 1750,5000,0,6 @08:08:10.74 Gate.setIR 0,5000,0,6 @08:08:10.78 Loaded Archive Cartridge @08:08:10.79 Gate.power :cartridge,:OFF @08:08:26.56 -> Cmd.startFiltering @08:08:27.14 Duration of filtering limited to 2:05:00 @08:08:27.39 Priming sample loop w/100ml, bypass w/10ml @08:08:27.41 SamplePump.setPosition! 0ml @08:08:27.45 Bypass.open @08:08:28.93 Intake.open @08:08:29.73 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:08:32.57 Vacuum tare is 52% of full scale @08:08:32.70 Pressure.reconfigure :default @08:08:32.76 Pressure.hold @08:08:32.93 SamplePump.reconfigure :prime @08:08:33.00 SamplePump.seek 10ml,1:21 @08:08:41.96 Pressure.coast @08:08:42.01 SamplePump.setPosition! 0ml @08:08:42.06 Exhaust.open @08:08:43.49 Bypass.close @08:08:45.01 Pressure.reconfigure :default @08:08:45.07 Pressure.hold @08:08:45.29 SamplePump.reconfigure :prime @08:08:45.36 SamplePump.seek 100ml,8:51 @08:10:39.23 Pressure.coast @08:10:39.29 Bypass.open @08:10:40.01 Intake.close @08:10:40.85 Toroid.reconfigure :hold @08:10:40.91 Toroid.seek :crack @08:10:41.71 Toroid.reconfigure :default @08:10:41.77 Toroid.seek :clear @08:10:43.34 Toroid.reconfigure :hold @08:10:43.40 Toroid.seek :cartridge @08:10:45.21 Toroid.reconfigure :default @08:10:45.27 Toroid.seek :clear @08:10:47.23 Pressurizing cartridge to 26psi above 15.7psia @08:10:47.25 SamplePump.setPosition! 0ml @08:10:47.29 Intake.open @08:10:48.02 Bypass.close @08:10:48.78 Exhaust.close @08:10:49.56 Pressure.reconfigure :default @08:10:49.61 Pressure.hold @08:10:49.78 SamplePump.reconfigure :charge @08:10:49.85 SamplePump.seek 2.3ml,42 seconds @08:10:57.51 Pressure.coast @08:10:57.56 Intake.close @08:10:58.35 Pressurized cartridge with 0.272ml to 26.0psi @08:10:58.36 Isolated @38.4psia (22.7psi) @08:10:58.37 Checking for cartridge leaks from 38.4psia @08:10:58.41 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @08:11:03.44 Cartridge pressure fell 2% to 37.7psia @08:11:03.58 Sampling 1000.0ml @08:11:03.59 SamplePump.setPosition! 0ml @08:11:03.65 Exhaust.open @08:11:04.34 Intake.open @08:11:05.19 Pressure.reconfigure :default @08:11:05.25 Pressure.hold @08:11:05.46 SamplePump.reconfigure :sample @08:11:05.54 SamplePump.seek 1000ml,1:23:53 @08:11:08.23 Cartridge 45 sampling 1/1000ml at 0.39ml/s with 20.7psi vacuum @08:12:08.23 Cartridge 45 sampling 23/1000ml at 0.36ml/s with 22.2psi vacuum @08:13:08.23 Cartridge 45 sampling 44/1000ml at 0.36ml/s with 24.0psi vacuum @08:14:08.23 Cartridge 45 sampling 62/1000ml at 0.29ml/s with 23.3psi vacuum @08:15:08.23 Cartridge 45 sampling 77/1000ml at 0.23ml/s with 24.7psi vacuum @08:16:08.23 Cartridge 45 sampling 90/1000ml at 0.20ml/s with 28.6psi vacuum @08:16:35.12 Flow averaged 0.30ml/s over 5:23 @08:16:35.15 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @08:16:35.84 Relieved excess vacuum in 7.5 seconds @08:16:35.87 SamplePump.seek 1000ml,1:15:53 @08:16:52.43 Flow averaged 0.25ml/s over 14 seconds @08:16:52.46 Waiting up to 15 seconds for vacuum to reduce from 28.7 to 18.0psi @08:16:53.16 Relieved excess vacuum in 4.2 seconds @08:16:53.18 SamplePump.seek 1000ml,1:15:37 @08:17:00.84 Flow estimate reduced to 0.20ml/s @08:17:00.87 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @08:17:01.56 Relieved excess vacuum in 5.3 seconds @08:17:01.59 SamplePump.seek 1000ml,1:15:29 @08:17:03.85 Flow estimate reduced to 0.16ml/s @08:17:03.86 Intake.close @08:17:03.92 Exhaust.close @08:17:04.09 Toroid.reconfigure :hold @08:17:04.16 Toroid.seek :bypass @08:17:06.15 Toroid.reconfigure :default @08:17:06.23 Toroid.seek :clear @08:17:07.86 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @08:17:07.87 Pressure.coast @08:17:08.05 Sampled 101.0ml @08:17:10.18 -> Cmd.startProcessing @08:17:10.43 Plunge.seek :reagent @08:17:11.30 Sample loop closed. Ready to Deliver Reagents @08:17:11.31 Archive.seek :sealed @08:17:15.58 Archive.seek :evac @08:17:29.52 delay 1:00 @08:18:29.54 Archive.seek :clear @08:18:47.26 RNAlater soak for 10:00 @08:28:47.29 Archive.seek :tVent @08:28:49.87 delay 1 second @08:28:50.89 Archive.seek :evac @08:29:06.50 delay 10 seconds @08:29:16.53 Archive.seek :tVent @08:29:32.15 delay 1 second @08:29:33.17 Archive.seek :evac @08:29:48.79 delay 10 seconds @08:29:58.82 Sample preserved and evacuated @08:29:59.79 -> Cmd.stop @08:29:59.93 Carousel.seek 45 @08:30:00.10 Plunge.seek :locked @08:30:00.94 PinPort.seek :clear @08:30:01.08 Archive.seek :clear @08:30:01.21 Air.seek :clear @08:30:01.34 Lysis1.seek :clear @08:30:18.82 Archive.coast @08:30:18.87 Air.coast @08:30:18.92 Lysis1.coast @08:30:18.97 Lysis2.coast @08:30:19.02 Plunge.coast @08:30:19.08 SamplePump.coast @08:30:19.12 Toroid.coast @08:30:19.17 Carousel.coast @08:30:19.23 PinPort.coast @08:30:19.41 Gate.power :motor,:OFF @08:30:19.43 Gate.power :core,:OFF @08:30:19.45 Gate.power :analytic1,:OFF @08:30:19.47 Gate.power :analytic2,:OFF @08:30:19.49 Gate.power :analytic3,:OFF @08:30:19.51 Gate.power :main,:OFF @08:30:19.53 Safely stopped and ready to power off ##-- file closed 2019-08-20T12:46:07.903Z ##-- file opened 2019-08-20T12:46:07.994Z @08:46:08.03 -> showStatus @08:46:10.04 -> Cmd.loadCartridge -15 @08:46:10.19 Gate.power :main,:ON,limit to 5000mA @08:46:10.20 Gate.power :analytic1,:ON,limit to 5000mA @08:46:10.22 Gate.power :analytic2,:ON,limit to 5000mA @08:46:10.24 Gate.power :analytic3,:ON,limit to 5000mA @08:46:10.38 Powering core bus @08:46:12.66 SPR Module 1, FW Rev. B, FPGA Rev. C @08:46:12.80 Archive.configure :default @08:46:12.92 Air.configure :default @08:46:13.02 Lysis1.configure :default @08:46:13.12 Lysis2.configure :default @08:46:13.24 Plunge.configure :default @08:46:13.36 SamplePump.configure :default @08:46:13.48 ExtraPump.configure :default @08:46:13.60 Toroid.configure :default @08:46:13.72 Carousel.configure :default @08:46:13.84 Pressure.configure :default @08:46:13.94 PinPort.configure :default @08:46:14.00 All elf controllers running firmware version 4.58 @08:46:14.03 Gate.power :motor,:ON,limit to 5000mA @08:46:14.23 Archive.reconfigure :default @08:46:14.51 Archive.home.jog 0 @08:46:14.64 PinPort.reconfigure :default @08:46:14.72 Toroid.reconfigure :default @08:46:14.76 Plunge.reconfigure :default @08:46:14.79 Lysis1.reconfigure :default @08:46:14.84 Lysis2.reconfigure :default @08:46:14.92 Air.reconfigure :default @08:46:15.12 Lysis2.home.jog 0 @08:46:15.17 PinPort.home.jog 0 @08:46:15.23 Plunge.home.jog 0 @08:46:15.28 Toroid.home.jog 0 @08:46:15.34 Air.home.jog 0 @08:46:15.40 Lysis1.home.jog 0 @08:46:15.69 Archive.reconfigure :homing @08:46:15.95 Toroid.reconfigure :homing @08:46:15.98 Plunge.reconfigure :homing @08:46:16.01 PinPort.reconfigure :homing @08:46:16.05 Lysis2.reconfigure :homing @08:46:16.08 Lysis1.reconfigure :homing @08:46:16.12 Air.reconfigure :homing @08:46:16.35 Air.jog 1401 @08:46:16.40 Plunge.jog 1542 @08:46:16.46 Toroid.jog 637 @08:46:16.51 Lysis1.jog 1399 @08:46:16.57 Lysis2.jog 1427 @08:46:16.63 Archive.jog 1382 @08:46:16.68 PinPort.jog -108 @08:46:16.92 PinPort.setRawPosition! 0 @08:46:16.96 PinPort.seek :home @08:46:17.05 PinPort.reconfigure :default @08:46:17.50 Air.setRawPosition! 50 @08:46:17.54 Air.seek :home @08:46:17.63 Plunge.setRawPosition! 50 @08:46:17.69 Plunge.seek :home @08:46:17.78 Lysis2.setRawPosition! 50 @08:46:17.81 Lysis1.setRawPosition! 50 @08:46:17.84 Archive.setRawPosition! 50 @08:46:17.92 Lysis2.seek :home @08:46:17.98 Lysis1.seek :home @08:46:18.01 Archive.seek :home @08:46:18.18 Plunge.reconfigure :default @08:46:18.20 Air.reconfigure :default @08:46:18.32 Lysis1.reconfigure :default @08:46:18.43 Archive.reconfigure :default @08:46:18.45 Lysis2.reconfigure :default @08:46:18.62 Toroid.setRawPosition! 0 @08:46:18.67 Toroid.seek :home @08:46:18.76 Toroid.reconfigure :default @08:46:18.90 Toroid.seek :clear @08:46:20.53 Carousel.reconfigure :default @08:46:20.60 Carousel.home.jog 0 @08:46:21.09 Carousel.seek 45 @08:46:21.25 Selecting Cartridge 42 @08:46:21.49 Carousel.seek 42 @08:46:22.38 Lysis1.seek :locked @08:46:26.51 Plunge.seek :locked @08:46:26.63 Air.seek :locked @08:46:30.76 Powering cartridge bus @08:46:32.88 Gate.setIR 1750,5000,0,6 @08:46:33.01 Gate.setIR 0,5000,0,6 @08:46:33.06 Loaded Lyse-n-Go Cartridge @08:46:33.08 PuckHeater.configure HeatConfig @08:46:33.13 Gate.power :heat,:ON,limit to 5000mA @08:46:49.05 -> Cmd.startFiltering @08:46:49.65 Duration of filtering limited to 2:05:00 @08:46:49.90 Priming sample loop w/100ml, bypass w/10ml @08:46:49.92 SamplePump.setPosition! 0ml @08:46:49.97 Bypass.open @08:46:51.49 Intake.open @08:46:52.34 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:46:55.18 Vacuum tare is 52% of full scale @08:46:55.31 Pressure.reconfigure :default @08:46:55.37 Pressure.hold @08:46:55.54 SamplePump.reconfigure :prime @08:46:55.61 SamplePump.seek 10ml,1:21 @08:47:04.56 Pressure.coast @08:47:04.62 SamplePump.setPosition! 0ml @08:47:04.66 Exhaust.open @08:47:06.09 Bypass.close @08:47:07.64 Pressure.reconfigure :default @08:47:07.70 Pressure.hold @08:47:07.92 SamplePump.reconfigure :prime @08:47:07.99 SamplePump.seek 100ml,8:51 @08:49:02.47 Pressure.coast @08:49:02.53 Bypass.open @08:49:03.26 Intake.close @08:49:04.10 Toroid.reconfigure :hold @08:49:04.17 Toroid.seek :crack @08:49:04.96 Toroid.reconfigure :default @08:49:05.02 Toroid.seek :clear @08:49:06.58 Toroid.reconfigure :hold @08:49:06.65 Toroid.seek :cartridge @08:49:08.46 Toroid.reconfigure :default @08:49:08.52 Toroid.seek :clear @08:49:10.50 Pressurizing cartridge to 26psi above 15.6psia @08:49:10.51 SamplePump.setPosition! 0ml @08:49:10.56 Intake.open @08:49:11.27 Bypass.close @08:49:12.01 Exhaust.close @08:49:12.79 Pressure.reconfigure :default @08:49:12.84 Pressure.hold @08:49:13.01 SamplePump.reconfigure :charge @08:49:13.08 SamplePump.seek 2.3ml,42 seconds @08:49:22.49 Pressure.coast @08:49:22.54 Intake.close @08:49:23.37 Pressurized cartridge with 0.413ml to 26.5psi @08:49:23.38 Isolated @38.9psia (23.3psi) @08:49:23.40 Checking for cartridge leaks from 38.9psia @08:49:23.43 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @08:49:28.46 Cartridge pressure fell 1% to 38.5psia @08:49:28.54 Sampling 1000.0ml @08:49:28.55 SamplePump.setPosition! 0ml @08:49:28.60 Exhaust.open @08:49:29.31 Intake.open @08:49:30.18 Pressure.reconfigure :default @08:49:30.24 Pressure.hold @08:49:30.46 SamplePump.reconfigure :sample @08:49:30.54 SamplePump.seek 1000ml,1:23:53 @08:49:33.22 Cartridge 42 sampling 1/1000ml at 0.56ml/s with 24.8psi vacuum @08:50:33.22 Cartridge 42 sampling 33/1000ml at 0.51ml/s with 25.7psi vacuum @08:51:33.22 Cartridge 42 sampling 64/1000ml at 0.47ml/s with 23.3psi vacuum @08:52:33.22 Cartridge 42 sampling 92/1000ml at 0.47ml/s with 25.9psi vacuum @08:53:33.22 Cartridge 42 sampling 118/1000ml at 0.45ml/s with 20.9psi vacuum @08:54:33.22 Cartridge 42 sampling 142/1000ml at 0.37ml/s with 28.4psi vacuum @08:55:33.22 Cartridge 42 sampling 165/1000ml at 0.33ml/s with 18.8psi vacuum @08:56:33.22 Cartridge 42 sampling 185/1000ml at 0.34ml/s with 23.6psi vacuum @08:57:33.22 Cartridge 42 sampling 203/1000ml at 0.32ml/s with 23.9psi vacuum @08:58:33.22 Cartridge 42 sampling 220/1000ml at 0.24ml/s with 24.8psi vacuum @08:59:33.22 Cartridge 42 sampling 235/1000ml at 0.27ml/s with 25.7psi vacuum @09:00:33.22 Cartridge 42 sampling 249/1000ml at 0.23ml/s with 22.3psi vacuum @09:01:33.22 Cartridge 42 sampling 262/1000ml at 0.21ml/s with 25.8psi vacuum @09:02:33.22 Cartridge 42 sampling 274/1000ml at 0.20ml/s with 25.1psi vacuum @09:03:27.60 Flow averaged 0.34ml/s over 13:53 @09:03:27.63 Waiting up to 16 seconds for vacuum to reduce from 29.3 to 18.0psi @09:03:28.32 Relieved excess vacuum in 4.9 seconds @09:03:28.35 SamplePump.seek 1000ml,1:00:08 @09:03:33.21 Cartridge 42 sampling 286/1000ml at 0.20ml/s with 29.8psi vacuum @09:03:42.91 Flow estimate reduced to 0.27ml/s @09:03:42.94 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @09:03:44.02 Relieved excess vacuum in 10.4 seconds @09:03:44.04 SamplePump.seek 1000ml,59:53 @09:04:03.19 Flow averaged 0.29ml/s over 14 seconds @09:04:03.22 Waiting up to 15 seconds for vacuum to reduce from 28.7 to 18.0psi @09:04:03.92 Relieved excess vacuum in 6.8 seconds @09:04:03.94 SamplePump.seek 1000ml,59:34 @09:04:13.72 Flow estimate reduced to 0.23ml/s @09:04:13.75 Waiting up to 15 seconds for vacuum to reduce from 28.7 to 18.0psi @09:04:14.46 Relieved excess vacuum in 7.4 seconds @09:04:14.48 SamplePump.seek 1000ml,59:25 @09:04:22.72 Flow estimate reduced to 0.19ml/s @09:04:22.74 Intake.close @09:04:22.81 Exhaust.close @09:04:22.97 Toroid.reconfigure :hold @09:04:23.04 Toroid.seek :bypass @09:04:24.66 Toroid.reconfigure :default @09:04:24.73 Toroid.seek :clear @09:04:26.35 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @09:04:26.37 Pressure.coast @09:04:26.56 Sampled 295.1ml @09:04:28.57 -> Cmd.startProcessing @09:04:30.43 SPR.command \"read_sequences\" @09:04:30.67 SPR.command \"stats_on\" @09:04:30.71 SPR.command \"start 0 1\" @09:04:32.72 Begun log /var/log/esp/spr19aug2009h.spr @09:04:33.52 Handoff.select :waste @09:04:33.71 Archive.seek :empty @09:04:40.91 Syringe tare is 20% of full scale @09:04:51.43 PinPort.seek :cartridge @09:04:55.99 Lysis1.seek 1350ul @09:04:58.46 Lysis2.seek 1700ul @09:05:01.56 Plunge.seek :reagent @09:05:02.40 Evacuating puck with filter bubble point of 50psi @09:05:02.41 PinPort.seek :cartridge @09:05:02.50 Handoff.select :waste @09:05:10.38 Air pumping 1 strokes to reach 8psi @09:05:10.40 Air.reconfigure :gulp @09:05:10.47 Air.seek :tVent @09:05:12.26 1.8psi after gulp @09:05:12.27 Air stroke #1 of 1 @09:05:12.29 Air.reconfigure :pressurize @09:05:12.37 Air.seek :pressurized @09:05:25.20 1.6psi after pressurize @09:05:25.21 Air.reconfigure :compress @09:05:25.29 Air.seek :evac @09:05:27.97 1.4psi after compress @09:05:41.85 0.3psi after compress (stable) @09:05:41.97 at 0.2psi Opened to waste @09:05:41.98 Handoff.select :blocked @09:05:42.16 Air pumping 10 strokes to reach 50psi @09:05:42.18 Air.reconfigure :gulp @09:05:42.26 Air.seek :tVent @09:05:52.15 9.0psi after gulp @09:05:52.16 Air stroke #1 of 10 @09:05:52.18 Air.reconfigure :pressurize @09:05:52.26 Air.seek :pressurized @09:06:05.08 12.6psi after pressurize @09:06:05.09 Air.reconfigure :compress @09:06:05.17 Air.seek :evac @09:06:07.83 14.5psi after compress @09:06:46.47 37.3psi after compress (stable) @09:06:46.49 Air.reconfigure :gulp @09:06:46.57 Air.seek :tVent @09:06:56.45 38.9psi after gulp @09:06:56.46 Air stroke #2 of 10 @09:06:56.48 Air.reconfigure :pressurize @09:06:56.56 Air.seek :pressurized @09:07:09.39 36.6psi after pressurize @09:07:09.40 Air.reconfigure :compress @09:07:09.49 Air.seek :evac @09:07:12.15 35.4psi after compress @09:07:24.35 Reached 50.0 of 50.0psi after pumping 4672ul (3.00 strokes) @09:07:24.37 Handoff.select :waste @09:07:46.60 delay 30 seconds @09:08:16.63 Handoff.select :blocked @09:08:41.61 Handoff.select :waste @09:08:41.73 PSI at @6.71psi at evacEnd @09:08:41.74 Handoff.select :blocked @09:08:41.87 Air.reconfigure :gulp @09:08:41.98 Air.seek :tVent @09:08:59.06 Stable @4.9psi after Handoff blocked @09:08:59.15 Air pumping 10 strokes to reach 35psi @09:08:59.16 Air stroke #1 of 10 @09:08:59.18 Air.reconfigure :pressurize @09:08:59.25 Air.seek :pressurized @09:09:12.10 8.6psi after pressurize @09:09:12.12 Air.reconfigure :compress @09:09:12.21 Air.seek :evac @09:09:14.88 9.1psi after compress @09:10:10.20 24.5psi after compress (stable) @09:10:10.22 Air.reconfigure :gulp @09:10:10.30 Air.seek :tVent @09:10:20.20 25.0psi after gulp @09:10:20.21 Air stroke #2 of 10 @09:10:20.23 Air.reconfigure :pressurize @09:10:20.32 Air.seek :pressurized @09:10:33.14 24.7psi after pressurize @09:10:33.15 Air.reconfigure :compress @09:10:33.23 Air.seek :evac @09:10:35.90 25.4psi after compress @09:10:55.94 34.6psi after compress (stable) @09:10:55.96 Air.reconfigure :gulp @09:10:56.03 Air.seek :tVent @09:10:56.20 Reached 34.8 of 34.9psi after pumping 3115ul (2.00 strokes) @09:10:56.22 Handoff.select :waste @09:11:15.00 delay 30 seconds @09:11:45.03 Handoff.select :blocked @09:12:10.03 Handoff.select :waste @09:12:10.15 PSI at @2.78psi at evacEnd @09:12:10.16 Air.reconfigure :default @09:12:10.28 Air.seek :fVent @09:12:11.71 delay 5 seconds @09:12:16.73 Seawater evacuated, Presure above/below filter released. Lysis begins @09:12:16.81 Lysis1.reconfigure :slow @09:12:16.87 Lysis1.seek 1050ul @09:12:46.87 delay 5 seconds @09:12:51.89 Handoff.select :blocked @09:12:52.05 Lysis1.seek 450ul @09:13:51.98 delay 3 seconds @09:13:55.05 Air.reconfigure to mix into 1.37806 parts Lysis1 @09:13:55.54 Air.seek :evac @09:13:55.58 Lysis1.seek 300ul @09:14:10.68 Air.reconfigure :default @09:14:10.74 Air.seek :full @09:14:24.95 Lysis1.seek :empty @09:14:55.04 Lysis Delivered. Syringe pressure @28.6psi @09:14:55.08 PuckHeater.seek 84.1C @09:15:48.00 PuckHeater.hold 15:00,84.1C @09:30:47.62 Lysis complete. Syringe pressure @36.9psi @09:30:47.63 Handoff.select :blocked @09:30:47.74 Lysis1.reconfigure :halfSpeed @09:30:47.80 Lysis1.seek 200ul @09:30:51.59 delay 1 second @09:30:52.61 Lysis1.seek :empty @09:30:56.44 Initial AirS pressure @37.4psi @09:30:56.50 Lysis1.reconfigure :extract @09:30:56.58 Lysis1.seek 900ul,10:00 @09:30:57.81 Air pumping 10 strokes to reach 21psi @09:30:57.82 Air stroke #1 of 10 @09:30:57.84 Air.reconfigure :pressurize @09:30:57.92 Air.seek :pressurized @09:31:10.75 15.5psi after pressurize @09:31:10.76 Air.reconfigure :compress @09:31:10.84 Air.seek :evac @09:31:13.52 17.5psi after compress @09:31:27.79 15.8psi after compress (stable) @09:31:27.81 Air.reconfigure :gulp @09:31:27.88 Air.seek :tVent @09:31:37.77 15.7psi after gulp @09:31:37.78 Air stroke #2 of 10 @09:31:37.80 Air.reconfigure :pressurize @09:31:37.88 Air.seek :pressurized @09:31:50.70 15.8psi after pressurize @09:31:50.71 Air.reconfigure :compress @09:31:50.79 Air.seek :evac @09:31:53.47 16.1psi after compress @09:32:07.38 15.8psi after compress (stable) @09:32:07.40 Air.reconfigure :gulp @09:32:07.47 Air.seek :tVent @09:32:17.36 16.0psi after gulp @09:32:17.37 Air stroke #3 of 10 @09:32:17.39 Air.reconfigure :pressurize @09:32:17.46 Air.seek :pressurized @09:32:30.29 15.6psi after pressurize @09:32:30.30 Air.reconfigure :compress @09:32:30.38 Air.seek :evac @09:32:33.04 15.5psi after compress @09:32:46.79 Air.reconfigure :default @09:32:46.84 Air.hold @09:32:47.01 Lysis1 at 900ul @16.3psi @09:32:47.04 Lysis1.reconfigure :default @09:32:47.12 Lysis1.seek 925ul @09:32:47.89 Lysis1 at 925ul, bottom @10.2psi after recovery @09:32:49.03 Lysis1 at 925ul, bottom @19.6psi @09:32:49.05 Air.reconfigure :halfSpeed @09:32:49.12 Air.seek :fVent @09:32:51.37 Puck Top Vented, bottom @21.5psi @09:32:51.38 Handoff.select :waste @09:32:51.50 delay 5 seconds @09:32:56.52 delay 5 seconds @09:33:01.54 Handoff.select SPR @09:33:01.65 SPR.command \"start 2 14\" @09:33:02.73 delay 10 seconds @09:33:12.75 SPR.command \"start 2 0\" @09:33:15.99 Waiting up to 11:00 for SPR to enter state 1 @09:41:48.06 SPR.command \"hold_on\" @09:41:49.45 SPR Dilution 1 of 1 -- Running Experiment 2 @09:41:49.46 Lysis1.reconfigure :DAmix @09:41:49.58 Lysis2.reconfigure :DAmix @09:41:49.99 Lysis1.seek 675ul @09:41:50.07 Lysis2.seek 1450ul @09:42:25.43 Lysis1.seek 375ul @09:42:25.47 Lysis2.seek 1150ul @09:43:07.50 volL1 = 300.0 and volL2 = 300.0 @09:43:07.52 delay 15 seconds @09:43:22.55 SPR.command \"hold_off\" @09:43:25.11 Waiting up to 25:00 for SPR to enter state 7 @09:55:01.64 SPR Analysis has Completed @09:55:01.65 Handoff.select :waste @09:55:02.55 Ended log /var/log/esp/spr19aug2009h.spr @09:55:02.56 SPR.command \"stats_off\" @09:55:04.15 -> Cmd.stop @09:55:04.24 Handoff.select :close @09:55:04.37 Gate.power :heat,:OFF @09:55:04.39 Gate.power :cartridge,:OFF @09:55:04.47 Carousel.seek 42 @09:55:04.64 Plunge.seek :locked @09:55:05.48 PinPort.seek :clear @09:55:10.09 Archive.seek :clear @09:55:10.22 Air.reconfigure :default @09:55:10.36 Lysis1.reconfigure :default @09:55:10.47 Air.seek :clear @09:55:10.53 Lysis1.seek :clear @09:55:10.62 Lysis2.reconfigure :default @09:55:10.70 Lysis2.seek :clear @09:55:27.84 Archive.coast @09:55:27.89 Air.coast @09:55:27.94 Lysis1.coast @09:55:27.99 Lysis2.coast @09:55:28.04 Plunge.coast @09:55:28.09 SamplePump.coast @09:55:28.14 Toroid.coast @09:55:28.19 Carousel.coast @09:55:28.24 PinPort.coast @09:55:28.43 Gate.power :motor,:OFF @09:55:28.45 Gate.power :core,:OFF @09:55:28.47 Gate.power :analytic1,:OFF @09:55:28.49 Gate.power :analytic2,:OFF @09:55:28.51 Gate.power :analytic3,:OFF @09:55:28.53 Gate.power :main,:OFF @09:55:28.55 Safely stopped and ready to power off ##-- file closed 2019-08-20T17:05:51.586Z