##-- file opened 2019-08-17T14:57:54.059Z @10:57:54.44 -> showStatus @10:57:56.52 -> Cmd.loadCartridge -4 @10:57:56.67 Gate.power :main,:ON,limit to 5000mA @10:57:56.68 Gate.power :analytic1,:ON,limit to 5000mA @10:57:56.70 Gate.power :analytic2,:ON,limit to 5000mA @10:57:56.73 Gate.power :analytic3,:ON,limit to 5000mA @10:57:56.86 Powering core bus @10:57:59.14 SPR Module 1, FW Rev. B, FPGA Rev. C @10:57:59.27 Archive.configure :default @10:57:59.39 Air.configure :default @10:57:59.49 Lysis1.configure :default @10:57:59.59 Lysis2.configure :default @10:57:59.71 Plunge.configure :default @10:57:59.83 SamplePump.configure :default @10:57:59.95 ExtraPump.configure :default @10:58:00.07 Toroid.configure :default @10:58:00.19 Carousel.configure :default @10:58:00.31 Pressure.configure :default @10:58:00.42 PinPort.configure :default @10:58:00.48 All elf controllers running firmware version 4.58 @10:58:00.50 Gate.power :motor,:ON,limit to 5000mA @10:58:00.67 Archive.reconfigure :default @10:58:00.96 Archive.home.jog 0 @10:58:01.09 Toroid.reconfigure :default @10:58:01.16 Plunge.reconfigure :default @10:58:01.21 Lysis1.reconfigure :default @10:58:01.27 Air.reconfigure :default @10:58:01.32 PinPort.reconfigure :default @10:58:01.38 Lysis2.reconfigure :default @10:58:01.59 Lysis2.home.jog 0 @10:58:01.64 Plunge.home.jog 0 @10:58:01.70 Toroid.home.jog 0 @10:58:01.75 Lysis1.home.jog 0 @10:58:01.81 Air.home.jog 0 @10:58:01.87 PinPort.home.jog 0 @10:58:02.18 Archive.reconfigure :homing @10:58:02.35 PinPort.reconfigure :homing @10:58:02.43 Plunge.reconfigure :homing @10:58:02.48 Air.reconfigure :homing @10:58:02.51 Toroid.reconfigure :homing @10:58:02.55 Lysis1.reconfigure :homing @10:58:02.61 Lysis2.reconfigure :homing @10:58:02.82 Plunge.jog 1535 @10:58:02.87 Archive.jog 1381 @10:58:02.93 Air.jog 1409 @10:58:02.99 Lysis1.jog 1399 @10:58:03.05 Lysis2.jog 151 @10:58:03.11 PinPort.jog -108 @10:58:03.16 Toroid.jog 634 @10:58:03.34 PinPort.setRawPosition! 0 @10:58:03.38 PinPort.seek :home @10:58:03.49 Lysis2.setRawPosition! 50 @10:58:03.51 PinPort.reconfigure :default @10:58:03.60 Lysis2.seek :home @10:58:03.89 Lysis2.reconfigure :default @10:58:04.03 Plunge.setRawPosition! 50 @10:58:04.05 Archive.setRawPosition! 50 @10:58:04.15 Plunge.seek :home @10:58:04.18 Archive.seek :home @10:58:04.34 Lysis1.setRawPosition! 50 @10:58:04.35 Air.setRawPosition! 50 @10:58:04.42 Lysis1.seek :home @10:58:04.47 Air.seek :home @10:58:04.61 Plunge.reconfigure :default @10:58:04.63 Archive.reconfigure :default @10:58:04.76 Lysis1.reconfigure :default @10:58:04.85 Air.reconfigure :default @10:58:05.32 Toroid.setRawPosition! 0 @10:58:05.36 Toroid.seek :home @10:58:05.45 Toroid.reconfigure :default @10:58:05.58 Toroid.seek :clear @10:58:07.21 Carousel.reconfigure :default @10:58:07.28 Carousel.home.jog 0 @10:58:07.77 Carousel.seek 50 @10:58:07.91 Selecting Cartridge 51 @10:58:08.17 Carousel.seek 51 @10:58:08.66 Lysis1.seek :locked @10:58:12.80 Plunge.seek :locked @10:58:12.93 Air.seek :locked @10:58:17.06 Powering cartridge bus @10:58:19.18 Gate.setIR 1750,5000,0,6 @10:58:19.31 Gate.setIR 0,5000,0,6 @10:58:19.35 Loaded Archive Cartridge Gate.power :cartridge,:OFF @10:58:35.31 -> Cmd.startFiltering @10:58:35.54 Duration of filtering limited to 2:05:00 @10:58:35.79 Priming sample loop w/100ml, bypass w/10ml @10:58:35.81 SamplePump.setPosition! 0ml @10:58:35.85 Bypass.open @10:58:37.73 Intake.open @10:58:38.53 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:58:41.37 Vacuum tare is 52% of full scale @10:58:41.50 Pressure.reconfigure :default @10:58:41.56 Pressure.hold @10:58:41.73 SamplePump.reconfigure :prime @10:58:41.80 SamplePump.seek 10ml,1:21 @10:58:50.76 Pressure.coast @10:58:50.82 SamplePump.setPosition! 0ml @10:58:50.86 Exhaust.open @10:58:52.28 Bypass.close @10:58:53.84 Pressure.reconfigure :default @10:58:53.90 Pressure.hold @10:58:54.12 SamplePump.reconfigure :prime @10:58:54.19 SamplePump.seek 100ml,8:51 @11:00:47.23 Pressure.coast @11:00:47.28 Bypass.open @11:00:47.99 Intake.close @11:00:48.80 Toroid.reconfigure :hold @11:00:48.87 Toroid.seek :crack @11:00:49.66 Toroid.reconfigure :default @11:00:49.73 Toroid.seek :clear @11:00:51.28 Toroid.reconfigure :hold @11:00:51.35 Toroid.seek :cartridge @11:00:53.17 Toroid.reconfigure :default @11:00:53.24 Toroid.seek :clear @11:00:55.21 Pressurizing cartridge to 26psi above 15.2psia @11:00:55.22 SamplePump.setPosition! 0ml @11:00:55.26 Intake.open @11:00:55.96 Bypass.close @11:00:56.67 Exhaust.close @11:00:57.47 Pressure.reconfigure :default @11:00:57.53 Pressure.hold @11:00:57.70 SamplePump.reconfigure :charge @11:00:57.77 SamplePump.seek 2.3ml,42 seconds @11:01:06.68 Pressure.coast @11:01:06.73 Intake.close @11:01:07.52 Pressurized cartridge with 0.370ml to 26.3psi @11:01:07.53 Isolated @38.2psia (23.0psi) @11:01:07.54 Checking for cartridge leaks from 38.2psia @11:01:07.57 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @11:01:12.61 Cartridge pressure fell 1% to 37.9psia @11:01:12.69 Sampling 1000.0ml @11:01:12.70 SamplePump.setPosition! 0ml @11:01:12.75 Exhaust.open @11:01:13.46 Intake.open @11:01:14.32 Pressure.reconfigure :default @11:01:14.38 Pressure.hold @11:01:14.60 SamplePump.reconfigure :sample @11:01:14.67 SamplePump.seek 1000ml,1:23:53 @11:01:17.37 Cartridge 51 sampling 1/1000ml at 0.43ml/s with 26.1psi vacuum @11:02:17.36 Cartridge 51 sampling 24/1000ml at 0.40ml/s with 23.5psi vacuum @11:03:17.36 Cartridge 51 sampling 41/1000ml at 0.20ml/s with 26.6psi vacuum @11:04:17.36 Cartridge 51 sampling 54/1000ml at 0.20ml/s with 27.5psi vacuum @11:04:19.27 Flow averaged 0.30ml/s over 3:03 @11:04:19.30 Waiting up to 14 seconds for vacuum to reduce from 28.3 to 18.0psi @11:04:20.30 Relieved excess vacuum in 2.9 seconds @11:04:20.32 SamplePump.seek 1000ml,1:19:21 @11:04:23.56 Flow estimate reduced to 0.24ml/s @11:04:23.58 Waiting up to 15 seconds for vacuum to reduce from 28.9 to 18.0psi @11:04:24.59 Relieved excess vacuum in 1.2 seconds @11:04:24.61 SamplePump.seek 1000ml,1:19:18 @11:04:26.68 Flow estimate reduced to 0.19ml/s @11:04:26.69 Intake.close @11:04:26.74 Exhaust.close @11:04:26.92 Toroid.reconfigure :hold @11:04:26.99 Toroid.seek :bypass @11:04:28.62 Toroid.reconfigure :default @11:04:28.69 Toroid.seek :clear @11:04:30.31 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:04:30.33 Pressure.coast @11:04:30.51 Sampled 55.4ml @11:04:32.68 -> Cmd.startProcessing @11:04:32.93 Plunge.seek :reagent @11:04:33.79 Sample loop closed. Ready to Deliver Reagents @11:04:33.80 Archive.seek :sealed @11:04:38.50 Archive.seek :evac @11:04:52.45 delay 1:00 @11:05:52.48 Archive.seek :clear @11:06:10.20 RNAlater soak for 10:00 @11:16:10.23 Archive.seek :tVent @11:16:12.81 delay 1 second @11:16:13.83 Archive.seek :evac @11:16:29.45 delay 10 seconds @11:16:39.48 Archive.seek :tVent @11:16:55.10 delay 1 second @11:16:56.12 Archive.seek :evac @11:17:11.75 delay 10 seconds @11:17:21.78 Sample preserved and evacuated @11:17:22.87 -> Cmd.stop @11:17:23.01 Carousel.seek 51 @11:17:23.19 Plunge.seek :locked @11:17:24.03 PinPort.seek :clear @11:17:24.17 Archive.seek :clear @11:17:24.30 Air.seek :clear @11:17:24.43 Lysis1.seek :clear @11:17:41.94 Archive.coast @11:17:41.99 Air.coast @11:17:42.03 Lysis1.coast @11:17:42.08 Lysis2.coast @11:17:42.13 Plunge.coast @11:17:42.18 SamplePump.coast @11:17:42.23 Toroid.coast @11:17:42.28 Carousel.coast @11:17:42.33 PinPort.coast @11:17:42.52 Gate.power :motor,:OFF @11:17:42.54 Gate.power :core,:OFF @11:17:42.56 Gate.power :analytic1,:OFF @11:17:42.58 Gate.power :analytic2,:OFF @11:17:42.60 Gate.power :analytic3,:OFF @11:17:42.62 Gate.power :main,:OFF @11:17:42.64 Safely stopped and ready to power off ##-- file closed 2019-08-17T15:33:30.443Z ##-- file opened 2019-08-17T15:33:30.445Z @11:33:30.66 -> showStatus @11:33:32.68 -> Cmd.loadCartridge -15 @11:33:32.83 Gate.power :main,:ON,limit to 5000mA @11:33:32.84 Gate.power :analytic1,:ON,limit to 5000mA @11:33:32.86 Gate.power :analytic2,:ON,limit to 5000mA @11:33:32.88 Gate.power :analytic3,:ON,limit to 5000mA @11:33:33.02 Powering core bus @11:33:35.30 SPR Module 1, FW Rev. B, FPGA Rev. C @11:33:35.44 Archive.configure :default @11:33:35.56 Air.configure :default @11:33:35.66 Lysis1.configure :default @11:33:35.76 Lysis2.configure :default @11:33:35.88 Plunge.configure :default @11:33:36.00 SamplePump.configure :default @11:33:36.12 ExtraPump.configure :default @11:33:36.24 Toroid.configure :default @11:33:36.36 Carousel.configure :default @11:33:36.48 Pressure.configure :default @11:33:36.59 PinPort.configure :default @11:33:36.65 All elf controllers running firmware version 4.58 @11:33:36.67 Gate.power :motor,:ON,limit to 5000mA @11:33:36.83 Archive.reconfigure :default @11:33:37.18 Archive.home.jog 0 @11:33:37.31 Toroid.reconfigure :default @11:33:37.40 Plunge.reconfigure :default @11:33:37.43 Lysis1.reconfigure :default @11:33:37.48 PinPort.reconfigure :default @11:33:37.54 Lysis2.reconfigure :default @11:33:37.57 Air.reconfigure :default @11:33:37.79 Air.home.jog 0 @11:33:37.83 Plunge.home.jog 0 @11:33:37.89 Lysis1.home.jog 0 @11:33:37.95 Toroid.home.jog 0 @11:33:38.01 Lysis2.home.jog 0 @11:33:38.07 PinPort.home.jog 0 @11:33:38.36 Archive.reconfigure :homing @11:33:38.60 Toroid.reconfigure :homing @11:33:38.62 Plunge.reconfigure :homing @11:33:38.65 PinPort.reconfigure :homing @11:33:38.70 Lysis1.reconfigure :homing @11:33:38.73 Air.reconfigure :homing @11:33:38.77 Lysis2.reconfigure :homing @11:33:38.98 Air.jog 1398 @11:33:39.04 Plunge.jog 1538 @11:33:39.09 Toroid.jog 629 @11:33:39.15 Lysis2.jog 1430 @11:33:39.22 PinPort.jog -107 @11:33:39.27 Lysis1.jog 1399 @11:33:39.33 Archive.jog 1385 @11:33:39.45 PinPort.setRawPosition! 0 @11:33:39.50 PinPort.seek :home @11:33:39.58 PinPort.reconfigure :default @11:33:40.14 Air.setRawPosition! 50 @11:33:40.18 Air.seek :home @11:33:40.29 Plunge.setRawPosition! 50 @11:33:40.34 Plunge.seek :home @11:33:40.44 Lysis2.setRawPosition! 50 @11:33:40.49 Lysis2.seek :home @11:33:40.59 Air.reconfigure :default @11:33:40.63 Archive.setRawPosition! 50 @11:33:40.66 Lysis1.setRawPosition! 50 @11:33:40.75 Archive.seek :home @11:33:40.80 Lysis1.seek :home @11:33:40.95 Plunge.reconfigure :default @11:33:40.98 Lysis2.reconfigure :default @11:33:41.15 Lysis1.reconfigure :default @11:33:41.17 Archive.reconfigure :default @11:33:41.30 Toroid.setRawPosition! 0 @11:33:41.35 Toroid.seek :home @11:33:41.44 Toroid.reconfigure :default @11:33:41.60 Toroid.seek :clear @11:33:43.22 Carousel.reconfigure :default @11:33:43.29 Carousel.home.jog 0 @11:33:43.78 Carousel.seek 51 @11:33:43.93 Selecting Cartridge 48 @11:33:44.17 Carousel.seek 48 @11:33:45.05 Lysis1.seek :locked @11:33:49.19 Plunge.seek :locked @11:33:49.31 Air.seek :locked @11:33:53.44 Powering cartridge bus @11:33:55.56 Gate.setIR 1750,5000,0,6 @11:33:55.69 Gate.setIR 0,5000,0,6 @11:33:55.74 Loaded Lyse-n-Go Cartridge @11:33:55.76 PuckHeater.configure HeatConfig @11:33:55.81 Gate.power :heat,:ON,limit to 5000mA @11:34:11.78 -> Cmd.startFiltering @11:34:12.01 Duration of filtering limited to 2:05:00 @11:34:12.64 Priming sample loop w/100ml, bypass w/10ml @11:34:12.66 SamplePump.setPosition! 0ml @11:34:12.70 Bypass.open @11:34:14.17 Intake.open @11:34:14.97 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:34:17.82 Vacuum tare is 52% of full scale @11:34:17.94 Pressure.reconfigure :default @11:34:18.00 Pressure.hold @11:34:18.17 SamplePump.reconfigure :prime @11:34:18.24 SamplePump.seek 10ml,1:21 @11:34:27.19 Pressure.coast @11:34:27.25 SamplePump.setPosition! 0ml @11:34:27.30 Exhaust.open @11:34:28.70 Bypass.close @11:34:30.22 Pressure.reconfigure :default @11:34:30.28 Pressure.hold @11:34:30.50 SamplePump.reconfigure :prime @11:34:30.58 SamplePump.seek 100ml,8:51 @11:36:23.65 Pressure.coast @11:36:23.70 Bypass.open @11:36:24.44 Intake.close @11:36:25.27 Toroid.reconfigure :hold @11:36:25.33 Toroid.seek :crack @11:36:26.13 Toroid.reconfigure :default @11:36:26.19 Toroid.seek :clear @11:36:27.75 Toroid.reconfigure :hold @11:36:27.82 Toroid.seek :cartridge @11:36:29.65 Toroid.reconfigure :default @11:36:29.71 Toroid.seek :clear @11:36:31.69 Pressurizing cartridge to 26psi above 15.4psia @11:36:31.70 SamplePump.setPosition! 0ml @11:36:31.74 Intake.open @11:36:32.48 Bypass.close @11:36:33.23 Exhaust.close @11:36:34.02 Pressure.reconfigure :default @11:36:34.07 Pressure.hold @11:36:34.24 SamplePump.reconfigure :charge @11:36:34.32 SamplePump.seek 2.3ml,42 seconds @11:36:44.54 Pressure.coast @11:36:44.59 Intake.close @11:36:45.42 Pressurized cartridge with 0.489ml to 26.6psi @11:36:45.43 Isolated @38.5psia (23.1psi) @11:36:45.44 Checking for cartridge leaks from 38.5psia @11:36:45.47 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @11:36:50.56 Sampling 1000.0ml @11:36:50.57 SamplePump.setPosition! 0ml @11:36:50.62 Exhaust.open @11:36:51.32 Intake.open @11:36:52.15 Pressure.reconfigure :default @11:36:52.21 Pressure.hold @11:36:52.43 SamplePump.reconfigure :sample @11:36:52.50 SamplePump.seek 1000ml,1:23:53 @11:36:55.20 Cartridge 48 sampling 1/1000ml at 0.54ml/s with 24.8psi vacuum @11:37:55.19 Cartridge 48 sampling 34/1000ml at 0.52ml/s with 23.8psi vacuum @11:38:55.19 Cartridge 48 sampling 65/1000ml at 0.49ml/s with 26.3psi vacuum @11:39:55.19 Cartridge 48 sampling 95/1000ml at 0.46ml/s with 24.5psi vacuum @11:40:55.19 Cartridge 48 sampling 124/1000ml at 0.44ml/s with 22.0psi vacuum @11:41:55.19 Cartridge 48 sampling 151/1000ml at 0.44ml/s with 24.2psi vacuum @11:42:55.19 Cartridge 48 sampling 177/1000ml at 0.42ml/s with 29.8psi vacuum @11:43:55.19 Cartridge 48 sampling 202/1000ml at 0.43ml/s with 26.4psi vacuum @11:44:55.19 Cartridge 48 sampling 226/1000ml at 0.40ml/s with 27.2psi vacuum @11:45:55.19 Cartridge 48 sampling 248/1000ml at 0.36ml/s with 23.7psi vacuum @11:46:55.19 Cartridge 48 sampling 268/1000ml at 0.29ml/s with 24.5psi vacuum @11:47:55.19 Cartridge 48 sampling 288/1000ml at 0.33ml/s with 26.9psi vacuum @11:48:55.19 Cartridge 48 sampling 305/1000ml at 0.29ml/s with 24.2psi vacuum @11:49:55.19 Cartridge 48 sampling 322/1000ml at 0.27ml/s with 22.7psi vacuum @11:50:55.19 Cartridge 48 sampling 337/1000ml at 0.26ml/s with 24.7psi vacuum @11:51:55.19 Cartridge 48 sampling 351/1000ml at 0.24ml/s with 24.8psi vacuum @11:52:55.19 Cartridge 48 sampling 364/1000ml at 0.20ml/s with 25.4psi vacuum @11:53:55.19 Cartridge 48 sampling 377/1000ml at 0.20ml/s with 25.1psi vacuum @11:54:55.19 Cartridge 48 sampling 389/1000ml at 0.20ml/s with 22.1psi vacuum @11:55:55.19 Cartridge 48 sampling 401/1000ml at 0.20ml/s with 25.4psi vacuum @11:56:37.39 Flow averaged 0.35ml/s over 19:43 @11:56:37.42 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @11:56:38.11 Relieved excess vacuum in 2.8 seconds @11:56:38.14 SamplePump.seek 1000ml,49:44 @11:56:55.19 Cartridge 48 sampling 413/1000ml at 0.20ml/s with 26.9psi vacuum @11:56:59.42 Flow averaged 0.24ml/s over 18 seconds @11:56:59.46 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @11:57:00.17 Relieved excess vacuum in 4.9 seconds @11:57:00.19 SamplePump.seek 1000ml,49:23 @11:57:14.67 Flow averaged 0.22ml/s over 14 seconds @11:57:14.70 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi @11:57:15.40 Relieved excess vacuum in 2.1 seconds @11:57:15.42 SamplePump.seek 1000ml,49:09 @11:57:25.19 Flow estimate reduced to 0.18ml/s @11:57:25.22 Intake.close @11:57:25.27 Exhaust.close @11:57:25.43 Toroid.reconfigure :hold @11:57:25.50 Toroid.seek :bypass @11:57:27.12 Toroid.reconfigure :default @11:57:27.19 Toroid.seek :clear @11:57:28.81 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:57:28.83 Pressure.coast @11:57:29.01 Sampled 418.5ml @11:57:31.07 -> Cmd.startProcessing @11:57:32.92 SPR.command \"read_sequences\" @11:57:32.95 SPR.command \"stats_on\" @11:57:32.99 SPR.command \"start 0 1\" @11:57:35.00 Begun log /var/log/esp/spr19aug1711h.spr @11:57:35.71 Handoff.select :waste @11:57:35.89 Archive.seek :empty @11:57:43.08 Syringe tare is 20% of full scale @11:57:53.61 PinPort.seek :cartridge @11:57:58.17 Lysis1.seek 1350ul @11:58:00.64 Lysis2.seek 1700ul @11:58:03.74 Plunge.seek :reagent @11:58:04.59 Evacuating puck with filter bubble point of 50psi @11:58:04.61 PinPort.seek :cartridge @11:58:04.69 Handoff.select :waste @11:58:12.02 Air pumping 1 strokes to reach 8psi @11:58:12.04 Air.reconfigure :gulp @11:58:12.11 Air.seek :tVent @11:58:13.93 1.8psi after gulp @11:58:13.94 Air stroke #1 of 1 @11:58:13.96 Air.reconfigure :pressurize @11:58:14.06 Air.seek :pressurized @11:58:26.89 2.1psi after pressurize @11:58:26.90 Air.reconfigure :compress @11:58:26.98 Air.seek :evac @11:58:29.64 1.4psi after compress @11:58:43.49 0.5psi after compress (stable) @11:58:43.61 at 0.5psi Opened to waste @11:58:43.62 Handoff.select :blocked @11:58:43.80 Air pumping 10 strokes to reach 50psi @11:58:43.81 Air.reconfigure :gulp @11:58:43.89 Air.seek :tVent @11:58:53.81 4.0psi after gulp @11:58:53.82 Air stroke #1 of 10 @11:58:53.84 Air.reconfigure :pressurize @11:58:53.92 Air.seek :pressurized @11:59:06.76 5.1psi after pressurize @11:59:06.77 Air.reconfigure :compress @11:59:06.84 Air.seek :evac @11:59:09.52 8.1psi after compress @11:59:33.16 20.0psi after compress (stable) @11:59:33.17 Air.reconfigure :gulp @11:59:33.25 Air.seek :tVent @11:59:43.14 20.4psi after gulp @11:59:43.15 Air stroke #2 of 10 @11:59:43.17 Air.reconfigure :pressurize @11:59:43.25 Air.seek :pressurized @11:59:56.09 20.3psi after pressurize @11:59:56.10 Air.reconfigure :compress @11:59:56.18 Air.seek :evac @11:59:58.86 24.1psi after compress @12:00:19.41 32.6psi after compress (stable) @12:00:19.43 Air.reconfigure :gulp @12:00:19.51 Air.seek :tVent @12:00:29.40 33.0psi after gulp @12:00:29.41 Air stroke #3 of 10 @12:00:29.43 Air.reconfigure :pressurize @12:00:29.50 Air.seek :pressurized @12:00:42.34 32.7psi after pressurize @12:00:42.35 Air.reconfigure :compress @12:00:42.43 Air.seek :evac @12:00:45.09 36.0psi after compress @12:01:04.34 43.2psi after compress (stable) @12:01:04.36 Air.reconfigure :gulp @12:01:04.44 Air.seek :tVent @12:01:14.34 43.6psi after gulp @12:01:14.35 Air stroke #4 of 10 @12:01:14.37 Air.reconfigure :pressurize @12:01:14.45 Air.seek :pressurized @12:01:27.29 43.3psi after pressurize @12:01:27.30 Air.reconfigure :compress @12:01:27.38 Air.seek :evac @12:01:30.04 46.0psi after compress @12:01:33.18 Reached 50.1 of 50.0psi after pumping 7787ul (5.00 strokes) @12:01:33.20 Handoff.select :waste @12:01:47.19 delay 30 seconds @12:02:17.21 Handoff.select :blocked @12:02:42.30 Handoff.select :waste @12:02:42.42 PSI at @0.41psi at evacEnd @12:02:42.43 Handoff.select :blocked @12:02:42.55 Air.reconfigure :gulp @12:02:42.66 Air.seek :tVent @12:02:59.74 Stable @0.6psi after Handoff blocked @12:02:59.82 Air pumping 10 strokes to reach 31psi @12:02:59.83 Air stroke #1 of 10 @12:02:59.86 Air.reconfigure :pressurize @12:02:59.93 Air.seek :pressurized @12:03:12.77 0.8psi after pressurize @12:03:12.78 Air.reconfigure :compress @12:03:12.86 Air.seek :evac @12:03:15.55 4.2psi after compress @12:03:32.61 11.6psi after compress (stable) @12:03:32.63 Air.reconfigure :gulp @12:03:32.70 Air.seek :tVent @12:03:42.60 11.8psi after gulp @12:03:42.61 Air stroke #2 of 10 @12:03:42.63 Air.reconfigure :pressurize @12:03:42.71 Air.seek :pressurized @12:03:55.56 11.8psi after pressurize @12:03:55.58 Air.reconfigure :compress @12:03:55.66 Air.seek :evac @12:03:58.36 16.1psi after compress @12:04:14.46 21.4psi after compress (stable) @12:04:14.48 Air.reconfigure :gulp @12:04:14.55 Air.seek :tVent @12:04:24.44 21.6psi after gulp @12:04:24.45 Air stroke #3 of 10 @12:04:24.47 Air.reconfigure :pressurize @12:04:24.55 Air.seek :pressurized @12:04:37.38 21.6psi after pressurize @12:04:37.39 Air.reconfigure :compress @12:04:37.47 Air.seek :evac @12:04:40.14 25.2psi after compress @12:04:57.40 30.4psi after compress (stable) @12:04:57.42 Air.reconfigure :gulp @12:04:57.50 Air.seek :tVent @12:04:57.68 Reached 30.4 of 30.6psi after pumping 4672ul (3.00 strokes) @12:04:57.69 Handoff.select :waste @12:05:07.08 delay 30 seconds @12:05:37.10 Handoff.select :blocked @12:06:02.13 Handoff.select :waste @12:06:02.25 PSI at @0.55psi at evacEnd @12:06:02.26 Air.reconfigure :default @12:06:02.38 Air.seek :fVent @12:06:03.82 delay 5 seconds @12:06:08.84 Seawater evacuated, Presure above/below filter released. Lysis begins @12:06:08.91 Lysis1.reconfigure :slow @12:06:08.98 Lysis1.seek 1050ul @12:06:39.01 delay 5 seconds @12:06:44.03 Handoff.select :blocked @12:06:44.20 Lysis1.seek 450ul @12:07:44.17 delay 3 seconds @12:07:47.26 Air.reconfigure to mix into 1.37806 parts Lysis1 @12:07:47.75 Air.seek :evac @12:07:47.80 Lysis1.seek 300ul @12:08:02.90 Air.reconfigure :default @12:08:02.96 Air.seek :full @12:08:17.18 Lysis1.seek :empty @12:08:47.29 Lysis Delivered. Syringe pressure @35.2psi @12:08:47.34 PuckHeater.seek 84.1C @12:09:30.57 PuckHeater.hold 15:00,84.1C @12:24:27.97 Lysis complete. Syringe pressure @43.3psi @12:24:27.98 Handoff.select :blocked @12:24:28.08 Lysis1.reconfigure :halfSpeed @12:24:28.15 Lysis1.seek 200ul @12:24:31.93 delay 1 second @12:24:32.95 Lysis1.seek :empty @12:24:36.75 Initial AirS pressure @42.5psi @12:24:36.82 Lysis1.reconfigure :extract @12:24:36.91 Lysis1.seek 900ul,10:00 @12:24:38.62 Air pumping 10 strokes to reach 21psi @12:24:38.63 Air stroke #1 of 10 @12:24:38.65 Air.reconfigure :pressurize @12:24:38.73 Air.seek :pressurized @12:24:51.59 16.2psi after pressurize @12:24:51.60 Air.reconfigure :compress @12:24:51.69 Air.seek :evac @12:24:54.35 16.7psi after compress @12:25:08.32 15.3psi after compress (stable) @12:25:08.33 Air.reconfigure :gulp @12:25:08.41 Air.seek :tVent @12:25:18.30 15.8psi after gulp @12:25:18.31 Air stroke #2 of 10 @12:25:18.33 Air.reconfigure :pressurize @12:25:18.41 Air.seek :pressurized @12:25:31.24 15.9psi after pressurize @12:25:31.28 Air.reconfigure :compress @12:25:31.36 Air.seek :evac @12:25:32.78 Air.reconfigure :default @12:25:32.84 Air.hold @12:25:33.37 Lysis1 at 900ul @19.7psi @12:25:33.39 Lysis1.reconfigure :default @12:25:33.46 Lysis1.seek 925ul @12:25:34.24 Lysis1 at 925ul, bottom @15.7psi after recovery @12:25:35.36 Lysis1 at 925ul, bottom @14.9psi @12:25:35.38 Air.reconfigure :halfSpeed @12:25:35.45 Air.seek :fVent @12:25:38.63 Puck Top Vented, bottom @2.6psi @12:25:38.64 Handoff.select :waste @12:25:38.75 delay 5 seconds @12:25:43.77 delay 5 seconds @12:25:48.79 Handoff.select SPR @12:25:48.90 SPR.command \"start 2 14\" @12:25:49.80 delay 10 seconds @12:25:59.82 SPR.command \"start 2 0\" @12:26:03.05 Waiting up to 11:00 for SPR to enter state 1 @12:34:35.14 SPR.command \"hold_on\" @12:34:36.51 SPR Dilution 1 of 1 -- Running Experiment 2 @12:34:36.52 Lysis1.reconfigure :DAmix @12:34:36.64 Lysis2.reconfigure :DAmix @12:34:36.98 Lysis1.seek 675ul @12:34:37.03 Lysis2.seek 1450ul @12:35:12.45 Lysis1.seek 375ul @12:35:12.50 Lysis2.seek 1150ul @12:35:54.53 volL1 = 300.0 and volL2 = 300.0 @12:35:54.56 delay 15 seconds @12:36:09.59 SPR.command \"hold_off\" @12:36:12.16 Waiting up to 25:00 for SPR to enter state 7 @12:47:48.69 SPR Analysis has Completed @12:47:48.71 Handoff.select :waste @12:47:49.61 Ended log /var/log/esp/spr19aug1711h.spr @12:47:49.62 SPR.command \"stats_off\" @12:47:51.14 -> Cmd.stop @12:47:51.23 Handoff.select :close @12:47:51.36 Gate.power :heat,:OFF @12:47:51.38 Gate.power :cartridge,:OFF @12:47:51.46 Carousel.seek 48 @12:47:51.63 Plunge.seek :locked @12:47:52.47 PinPort.seek :clear @12:47:57.09 Archive.seek :clear @12:47:57.22 Air.reconfigure :default @12:47:57.35 Lysis1.reconfigure :default @12:47:57.46 Air.seek :clear @12:47:57.52 Lysis1.seek :clear @12:47:57.60 Lysis2.reconfigure :default @12:47:57.68 Lysis2.seek :clear @12:48:14.84 Archive.coast @12:48:14.89 Air.coast @12:48:14.94 Lysis1.coast @12:48:14.99 Lysis2.coast @12:48:15.04 Plunge.coast @12:48:15.09 SamplePump.coast @12:48:15.14 Toroid.coast @12:48:15.19 Carousel.coast @12:48:15.25 PinPort.coast @12:48:15.43 Gate.power :motor,:OFF @12:48:15.44 Gate.power :core,:OFF @12:48:15.47 Gate.power :analytic1,:OFF @12:48:15.49 Gate.power :analytic2,:OFF @12:48:15.51 Gate.power :analytic3,:OFF @12:48:15.53 Gate.power :main,:OFF @12:48:15.55 Safely stopped and ready to power off ##-- file closed 2019-08-17T18:06:46.173Z ##-- file opened 2019-08-17T18:06:46.175Z @14:06:46.12 -> showStatus @14:06:48.14 -> Cmd.loadCartridge -4 @14:06:48.29 Gate.power :main,:ON,limit to 5000mA @14:06:48.30 Gate.power :analytic1,:ON,limit to 5000mA @14:06:48.32 Gate.power :analytic2,:ON,limit to 5000mA @14:06:48.35 Gate.power :analytic3,:ON,limit to 5000mA @14:06:48.48 Powering core bus @14:06:50.77 SPR Module 1, FW Rev. B, FPGA Rev. C @14:06:50.90 Archive.configure :default @14:06:51.02 Air.configure :default @14:06:51.16 Lysis1.configure :default @14:06:51.26 Lysis2.configure :default @14:06:51.38 Plunge.configure :default @14:06:51.50 SamplePump.configure :default @14:06:51.62 ExtraPump.configure :default @14:06:51.74 Toroid.configure :default @14:06:51.86 Carousel.configure :default @14:06:51.98 Pressure.configure :default @14:06:52.08 PinPort.configure :default @14:06:52.13 All elf controllers running firmware version 4.58 @14:06:52.16 Gate.power :motor,:ON,limit to 5000mA @14:06:52.57 Toroid.reconfigure :default @14:06:52.65 Plunge.reconfigure :default @14:06:52.68 Lysis1.reconfigure :default @14:06:52.71 Air.reconfigure :default @14:06:52.76 PinPort.reconfigure :default @14:06:52.79 Archive.reconfigure :default @14:06:52.83 Lysis2.reconfigure :default @14:06:53.08 Archive.home.jog 0 @14:06:53.13 Toroid.home.jog 0 @14:06:53.18 Air.home.jog 0 @14:06:53.24 PinPort.home.jog 0 @14:06:53.30 Lysis2.home.jog 0 @14:06:53.36 Plunge.home.jog 0 @14:06:53.42 Lysis1.home.jog 0 @14:06:53.84 PinPort.reconfigure :homing @14:06:53.93 Plunge.reconfigure :homing @14:06:53.98 Toroid.reconfigure :homing @14:06:54.04 Air.reconfigure :homing @14:06:54.07 Archive.reconfigure :homing @14:06:54.11 Lysis2.reconfigure :homing @14:06:54.34 Toroid.jog 629 @14:06:54.39 Lysis2.jog 1425 @14:06:54.45 Plunge.jog 1542 @14:06:54.51 Archive.jog 1384 @14:06:54.57 Air.jog 1412 @14:06:54.62 PinPort.jog -106 @14:06:54.70 Lysis1.reconfigure :homing @14:06:54.77 Lysis1.jog 1399 @14:06:54.85 PinPort.setRawPosition! 0 @14:06:54.89 PinPort.seek :home @14:06:54.98 PinPort.reconfigure :default @14:06:55.56 Lysis2.setRawPosition! 50 @14:06:55.60 Lysis2.seek :home @14:06:55.73 Air.setRawPosition! 50 @14:06:55.76 Archive.setRawPosition! 50 @14:06:55.78 Plunge.setRawPosition! 50 @14:06:55.86 Air.seek :home @14:06:55.94 Plunge.seek :home @14:06:55.99 Archive.seek :home @14:06:56.17 Lysis1.setRawPosition! 50 @14:06:56.19 Lysis2.reconfigure :default @14:06:56.28 Lysis1.seek :home @14:06:56.42 Plunge.reconfigure :default @14:06:56.45 Air.reconfigure :default @14:06:56.49 Archive.reconfigure :default @14:06:56.67 Lysis1.reconfigure :default @14:06:56.69 Toroid.setRawPosition! 0 @14:06:56.77 Toroid.seek :home @14:06:56.89 Toroid.reconfigure :default @14:06:57.11 Toroid.seek :clear @14:06:58.73 Carousel.reconfigure :default @14:06:58.80 Carousel.home.jog 0 @14:06:59.30 Carousel.seek 48 @14:06:59.47 Selecting Cartridge 49 @14:06:59.72 Carousel.seek 49 @14:07:00.21 Lysis1.seek :locked @14:07:04.34 Plunge.seek :locked @14:07:04.46 Air.seek :locked @14:07:08.65 Powering cartridge bus @14:07:10.77 Gate.setIR 1750,5000,0,6 @14:07:10.90 Gate.setIR 0,5000,0,6 @14:07:10.94 Loaded Archive Cartridge @14:07:10.95 Gate.power :cartridge,:OFF @14:07:26.67 -> Cmd.startFiltering @14:07:26.90 Duration of filtering limited to 2:05:00 @14:07:27.51 Priming sample loop w/100ml, bypass w/10ml @14:07:27.53 SamplePump.setPosition! 0ml @14:07:27.57 Bypass.open @14:07:29.10 Intake.open @14:07:29.91 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:07:32.75 Vacuum tare is 52% of full scale @14:07:32.88 Pressure.reconfigure :default @14:07:32.94 Pressure.hold @14:07:33.11 SamplePump.reconfigure :prime @14:07:33.19 SamplePump.seek 10ml,1:21 @14:07:42.15 Pressure.coast @14:07:42.20 SamplePump.setPosition! 0ml @14:07:42.24 Exhaust.open @14:07:43.65 Bypass.close @14:07:45.17 Pressure.reconfigure :default @14:07:45.23 Pressure.hold @14:07:45.45 SamplePump.reconfigure :prime @14:07:45.53 SamplePump.seek 100ml,8:51 @14:09:38.20 Pressure.coast @14:09:38.25 Bypass.open @14:09:38.97 Intake.close @14:09:39.78 Toroid.reconfigure :hold @14:09:39.85 Toroid.seek :crack @14:09:40.64 Toroid.reconfigure :default @14:09:40.71 Toroid.seek :clear @14:09:42.26 Toroid.reconfigure :hold @14:09:42.33 Toroid.seek :cartridge @14:09:44.14 Toroid.reconfigure :default @14:09:44.20 Toroid.seek :clear @14:09:46.18 Pressurizing cartridge to 26psi above 15.7psia @14:09:46.19 SamplePump.setPosition! 0ml @14:09:46.24 Intake.open @14:09:46.95 Bypass.close @14:09:47.67 Exhaust.close @14:09:48.46 Pressure.reconfigure :default @14:09:48.52 Pressure.hold @14:09:48.69 SamplePump.reconfigure :charge @14:09:48.76 SamplePump.seek 2.3ml,42 seconds @14:09:56.06 Pressure.coast @14:09:56.11 Intake.close @14:09:56.92 Pressurized cartridge with 0.247ml to 26.2psi @14:09:56.93 Isolated @37.8psia (22.1psi) @14:09:56.94 Checking for cartridge leaks from 37.8psia @14:09:56.97 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @14:10:02.02 Cartridge pressure fell 1% to 37.4psia @14:10:02.10 Sampling 1000.0ml @14:10:02.11 SamplePump.setPosition! 0ml @14:10:02.16 Exhaust.open @14:10:02.84 Intake.open @14:10:03.71 Pressure.reconfigure :default @14:10:03.77 Pressure.hold @14:10:03.99 SamplePump.reconfigure :sample @14:10:04.07 SamplePump.seek 1000ml,1:23:53 @14:10:06.75 Cartridge 49 sampling 1/1000ml at 0.39ml/s with 22.9psi vacuum @14:11:06.75 Cartridge 49 sampling 23/1000ml at 0.37ml/s with 23.3psi vacuum @14:12:06.75 Cartridge 49 sampling 41/1000ml at 0.31ml/s with 23.3psi vacuum @14:13:06.75 Cartridge 49 sampling 57/1000ml at 0.24ml/s with 25.1psi vacuum @14:14:06.75 Cartridge 49 sampling 69/1000ml at 0.20ml/s with 28.3psi vacuum @14:14:28.96 Flow averaged 0.28ml/s over 4:23 @14:14:28.99 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi @14:14:29.69 Relieved excess vacuum in 2.8 seconds @14:14:29.71 SamplePump.seek 1000ml,1:17:43 @14:14:40.06 Flow estimate reduced to 0.22ml/s @14:14:40.08 Waiting up to 17 seconds for vacuum to reduce from 29.6 to 18.0psi @14:14:40.79 Relieved excess vacuum in 8 seconds @14:14:40.81 SamplePump.seek 1000ml,1:17:33 @14:14:48.45 Flow estimate reduced to 0.18ml/s @14:14:48.46 Intake.close @14:14:48.52 Exhaust.close @14:14:48.69 Toroid.reconfigure :hold @14:14:48.76 Toroid.seek :bypass @14:14:50.37 Toroid.reconfigure :default @14:14:50.44 Toroid.seek :clear @14:14:52.05 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @14:14:52.07 Pressure.coast @14:14:52.26 Sampled 77.4ml @14:14:54.54 -> Cmd.startProcessing @14:14:54.79 Plunge.seek :reagent @14:14:56.02 Sample loop closed. Ready to Deliver Reagents @14:14:56.03 Archive.seek :sealed @14:15:00.31 Archive.seek :evac @14:15:14.26 delay 1:00 @14:16:14.29 Archive.seek :clear @14:16:32.02 RNAlater soak for 10:00 @14:26:32.05 Archive.seek :tVent @14:26:34.63 delay 1 second @14:26:35.65 Archive.seek :evac @14:26:51.27 delay 10 seconds @14:27:01.30 Archive.seek :tVent @14:27:16.92 delay 1 second @14:27:17.94 Archive.seek :evac @14:27:33.56 delay 10 seconds @14:27:43.59 Sample preserved and evacuated @14:27:44.57 -> Cmd.stop @14:27:44.71 Carousel.seek 49 @14:27:44.89 Plunge.seek :locked @14:27:45.73 PinPort.seek :clear @14:27:45.87 Archive.seek :clear @14:27:46.00 Air.seek :clear @14:27:46.13 Lysis1.seek :clear @14:28:03.62 Archive.coast @14:28:03.66 Air.coast @14:28:03.72 Lysis1.coast @14:28:03.76 Lysis2.coast @14:28:03.82 Plunge.coast @14:28:03.87 SamplePump.coast @14:28:03.92 Toroid.coast @14:28:03.97 Carousel.coast @14:28:04.02 PinPort.coast @14:28:04.20 Gate.power :motor,:OFF @14:28:04.22 Gate.power :core,:OFF @14:28:04.24 Gate.power :analytic1,:OFF @14:28:04.26 Gate.power :analytic2,:OFF @14:28:04.28 Gate.power :analytic3,:OFF @14:28:04.30 Gate.power :main,:OFF @14:28:04.32 Safely stopped and ready to power off ##-- file closed 2019-08-17T18:43:52.978Z ##-- file opened 2019-08-17T18:43:52.986Z @14:43:53.36 -> showStatus @14:43:55.37 -> Cmd.loadCartridge -15 @14:43:55.52 Gate.power :main,:ON,limit to 5000mA @14:43:55.53 Gate.power :analytic1,:ON,limit to 5000mA @14:43:55.55 Gate.power :analytic2,:ON,limit to 5000mA @14:43:55.58 Gate.power :analytic3,:ON,limit to 5000mA @14:43:55.71 Powering core bus @14:43:58.00 SPR Module 1, FW Rev. B, FPGA Rev. C @14:43:58.12 Archive.configure :default @14:43:58.24 Air.configure :default @14:43:58.35 Lysis1.configure :default @14:43:58.45 Lysis2.configure :default @14:43:58.57 Plunge.configure :default @14:43:58.69 SamplePump.configure :default @14:43:58.81 ExtraPump.configure :default @14:43:58.93 Toroid.configure :default @14:43:59.05 Carousel.configure :default @14:43:59.17 Pressure.configure :default @14:43:59.27 PinPort.configure :default @14:43:59.32 All elf controllers running firmware version 4.58 @14:43:59.35 Gate.power :motor,:ON,limit to 5000mA @14:43:59.46 Archive.reconfigure :default @14:43:59.81 Archive.home.jog 0 @14:43:59.99 Toroid.reconfigure :default @14:44:00.07 Plunge.reconfigure :default @14:44:00.11 Lysis1.reconfigure :default @14:44:00.17 Air.reconfigure :default @14:44:00.22 Lysis2.reconfigure :default @14:44:00.27 PinPort.reconfigure :default @14:44:00.48 Lysis2.home.jog 0 @14:44:00.54 Lysis1.home.jog 0 @14:44:00.59 Plunge.home.jog 0 @14:44:00.65 Toroid.home.jog 0 @14:44:00.71 PinPort.home.jog 0 @14:44:00.77 Air.home.jog 0 @14:44:01.09 Archive.reconfigure :homing @14:44:01.28 Toroid.reconfigure :homing @14:44:01.32 Plunge.reconfigure :homing @14:44:01.35 Air.reconfigure :homing @14:44:01.40 PinPort.reconfigure :homing @14:44:01.43 Lysis1.reconfigure :homing @14:44:01.47 Lysis2.reconfigure :homing @14:44:01.68 Air.jog 1404 @14:44:01.74 Lysis1.jog 1399 @14:44:01.79 Plunge.jog 1539 @14:44:01.85 Archive.jog 1379 @14:44:01.91 Toroid.jog 638 @14:44:01.97 PinPort.jog -106 @14:44:02.03 Lysis2.jog 1428 @14:44:02.20 PinPort.setRawPosition! 0 @14:44:02.24 PinPort.seek :home @14:44:02.33 PinPort.reconfigure :default @14:44:02.84 Air.setRawPosition! 50 @14:44:02.90 Air.seek :home @14:44:03.01 Plunge.setRawPosition! 50 @14:44:03.03 Archive.setRawPosition! 50 @14:44:03.07 Lysis1.setRawPosition! 50 @14:44:03.12 Plunge.seek :home @14:44:03.21 Archive.seek :home @14:44:03.26 Lysis1.seek :home @14:44:03.44 Lysis2.setRawPosition! 50 @14:44:03.46 Air.reconfigure :default @14:44:03.55 Lysis2.seek :home @14:44:03.68 Lysis1.reconfigure :default @14:44:03.71 Archive.reconfigure :default @14:44:03.74 Plunge.reconfigure :default @14:44:03.91 Lysis2.reconfigure :default @14:44:04.08 Toroid.setRawPosition! 0 @14:44:04.12 Toroid.seek :home @14:44:04.22 Toroid.reconfigure :default @14:44:04.36 Toroid.seek :clear @14:44:05.98 Carousel.reconfigure :default @14:44:06.05 Carousel.home.jog 0 @14:44:06.55 Carousel.seek 49 @14:44:06.71 Selecting Cartridge 46 @14:44:06.95 Carousel.seek 46 @14:44:07.84 Lysis1.seek :locked @14:44:11.97 Plunge.seek :locked @14:44:12.09 Air.seek :locked @14:44:16.22 Powering cartridge bus @14:44:18.34 Gate.setIR 1750,5000,0,6 @14:44:18.47 Gate.setIR 0,5000,0,6 @14:44:18.53 Loaded Lyse-n-Go Cartridge @14:44:18.54 PuckHeater.configure HeatConfig @14:44:18.59 Gate.power :heat,:ON,limit to 5000mA @14:44:34.48 -> Cmd.startFiltering @14:44:34.71 Duration of filtering limited to 2:05:00 @14:44:35.34 Priming sample loop w/100ml, bypass w/10ml @14:44:35.35 SamplePump.setPosition! 0ml @14:44:35.39 Bypass.open @14:44:36.88 Intake.open @14:44:37.69 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:44:40.52 Vacuum tare is 52% of full scale @14:44:40.65 Pressure.reconfigure :default @14:44:40.71 Pressure.hold @14:44:40.88 SamplePump.reconfigure :prime @14:44:40.95 SamplePump.seek 10ml,1:21 @14:44:49.90 Pressure.coast @14:44:49.96 SamplePump.setPosition! 0ml @14:44:50.01 Exhaust.open @14:44:51.42 Bypass.close @14:44:52.96 Pressure.reconfigure :default @14:44:53.02 Pressure.hold @14:44:53.24 SamplePump.reconfigure :prime @14:44:53.31 SamplePump.seek 100ml,8:51 @14:46:46.33 Pressure.coast @14:46:46.39 Bypass.open @14:46:47.13 Intake.close @14:46:47.95 Toroid.reconfigure :hold @14:46:48.01 Toroid.seek :crack @14:46:48.81 Toroid.reconfigure :default @14:46:48.87 Toroid.seek :clear @14:46:50.43 Toroid.reconfigure :hold @14:46:50.50 Toroid.seek :cartridge @14:46:52.31 Toroid.reconfigure :default @14:46:52.37 Toroid.seek :clear @14:46:54.34 Pressurizing cartridge to 26psi above 16.2psia @14:46:54.35 SamplePump.setPosition! 0ml @14:46:54.40 Intake.open @14:46:55.10 Bypass.close @14:46:55.82 Exhaust.close @14:46:56.62 Pressure.reconfigure :default @14:46:56.67 Pressure.hold @14:46:56.84 SamplePump.reconfigure :charge @14:46:56.91 SamplePump.seek 2.3ml,42 seconds @14:47:08.33 Pressure.coast @14:47:08.39 Intake.close @14:47:09.16 Pressurized cartridge with 0.611ml to 26.3psi @14:47:09.17 Isolated @38.2psia (22.0psi) @14:47:09.18 Checking for cartridge leaks from 38.2psia @14:47:09.21 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @14:47:14.24 Cartridge pressure fell 3% to 37.0psia @14:47:14.32 Sampling 1000.0ml @14:47:14.33 SamplePump.setPosition! 0ml @14:47:14.38 Exhaust.open @14:47:15.05 Intake.open @14:47:15.90 Pressure.reconfigure :default @14:47:15.96 Pressure.hold @14:47:16.18 SamplePump.reconfigure :sample @14:47:16.26 SamplePump.seek 1000ml,1:23:53 @14:47:18.94 Cartridge 46 sampling 1/1000ml at 0.53ml/s with 21.0psi vacuum @14:48:18.94 Cartridge 46 sampling 34/1000ml at 0.53ml/s with 22.4psi vacuum @14:49:18.94 Cartridge 46 sampling 65/1000ml at 0.53ml/s with 23.5psi vacuum @14:50:18.94 Cartridge 46 sampling 95/1000ml at 0.49ml/s with 17.6psi vacuum 2.8m deep (18.8psia) @14:51:18.94 Cartridge 46 sampling 123/1000ml at 0.45ml/s with 19.5psi vacuum 1.8m deep (17.4psia) @14:52:18.94 Cartridge 46 sampling 150/1000ml at 0.41ml/s with 17.5psi vacuum @14:53:18.94 Cartridge 46 sampling 175/1000ml at 0.42ml/s with 22.0psi vacuum @14:54:18.94 Cartridge 46 sampling 200/1000ml at 0.42ml/s with 26.8psi vacuum @14:55:18.94 Cartridge 46 sampling 223/1000ml at 0.38ml/s with 25.6psi vacuum @14:56:18.94 Cartridge 46 sampling 245/1000ml at 0.34ml/s with 16.9psi vacuum 1.6m deep (17.1psia) @14:57:18.94 Cartridge 46 sampling 266/1000ml at 0.36ml/s with 27.4psi vacuum @14:58:18.94 Cartridge 46 sampling 285/1000ml at 0.30ml/s with 16.5psi vacuum @14:59:18.94 Cartridge 46 sampling 303/1000ml at 0.32ml/s with 25.7psi vacuum @15:00:18.94 Cartridge 46 sampling 319/1000ml at 0.27ml/s with 20.1psi vacuum @15:01:18.94 Cartridge 46 sampling 334/1000ml at 0.25ml/s with 27.5psi vacuum @15:02:18.94 Cartridge 46 sampling 347/1000ml at 0.23ml/s with 20.7psi vacuum @15:03:18.94 Cartridge 46 sampling 360/1000ml at 0.20ml/s with 25.9psi vacuum @15:04:18.93 Cartridge 46 sampling 373/1000ml at 0.22ml/s with 19.8psi vacuum 2.4m deep (18.2psia) @15:05:18.94 Cartridge 46 sampling 385/1000ml at 0.20ml/s with 23.7psi vacuum @15:06:18.94 Cartridge 46 sampling 397/1000ml at 0.20ml/s with 24.2psi vacuum @15:07:13.95 Flow averaged 0.34ml/s over 19:53 @15:07:13.98 Waiting up to 13 seconds for vacuum to reduce from 27.8 to 18.0psi @15:07:14.68 Relieved excess vacuum in 5.6 seconds @15:07:14.70 SamplePump.seek 1000ml,49:49 @15:07:19.32 Cartridge 46 sampling 410/1000ml at 0.20ml/s with 29.1psi vacuum @15:07:41.77 Flow averaged 0.22ml/s over 25 seconds @15:07:41.80 Waiting up to 12 seconds for vacuum to reduce from 27.7 to 18.0psi @15:07:42.50 Relieved excess vacuum in 3.1 seconds @15:07:42.52 SamplePump.seek 1000ml,49:22 @15:08:03.31 Flow averaged 0.32ml/s over 14 seconds @15:08:03.34 Waiting up to 12 seconds for vacuum to reduce from 27.7 to 18.0psi @15:08:04.04 Relieved excess vacuum in 8.4 seconds @15:08:04.06 SamplePump.seek 1000ml,49:01 @15:08:18.93 Cartridge 46 sampling 421/1000ml at 0.20ml/s with 28.6psi vacuum @15:08:20.13 Flow averaged 0.21ml/s over 15 seconds @15:08:20.16 Waiting up to 15 seconds for vacuum to reduce from 28.8 to 18.0psi @15:08:20.86 Relieved excess vacuum in 1.9 seconds @15:08:20.88 SamplePump.seek 1000ml,48:45 @15:08:33.84 Flow estimate reduced to 0.17ml/s @15:08:33.85 Intake.close @15:08:33.90 Exhaust.close @15:08:34.07 Toroid.reconfigure :hold @15:08:34.14 Toroid.seek :bypass @15:08:35.76 Toroid.reconfigure :default @15:08:35.83 Toroid.seek :clear @15:08:37.45 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @15:08:37.47 Pressure.coast @15:08:37.65 Sampled 424.0ml @15:08:39.82 -> Cmd.startProcessing @15:08:41.67 SPR.command \"read_sequences\" @15:08:41.90 SPR.command \"stats_on\" @15:08:41.94 SPR.command \"start 0 1\" @15:08:43.95 Begun log /var/log/esp/spr19aug1715h.spr @15:08:44.75 Handoff.select :waste @15:08:44.91 Archive.seek :empty @15:08:52.16 Syringe tare is 20% of full scale @15:09:02.69 PinPort.seek :cartridge @15:09:07.25 Lysis1.seek 1350ul @15:09:09.72 Lysis2.seek 1700ul @15:09:12.82 Plunge.seek :reagent @15:09:13.65 Evacuating puck with filter bubble point of 50psi @15:09:13.67 PinPort.seek :cartridge @15:09:13.75 Handoff.select :waste @15:09:21.08 Air pumping 1 strokes to reach 8psi @15:09:21.10 Air.reconfigure :gulp @15:09:21.19 Air.seek :tVent @15:09:22.98 0.1psi after gulp @15:09:22.99 Air stroke #1 of 1 @15:09:23.01 Air.reconfigure :pressurize @15:09:23.10 Air.seek :pressurized @15:09:35.94 0.3psi after pressurize @15:09:35.95 Air.reconfigure :compress @15:09:36.03 Air.seek :evac @15:09:38.69 0.6psi after compress @15:09:52.56 -0.0psi after compress (stable) @15:09:52.68 at 0.0psi Opened to waste @15:09:52.69 Handoff.select :blocked @15:09:52.87 Air pumping 10 strokes to reach 50psi @15:09:52.88 Air.reconfigure :gulp @15:09:52.96 Air.seek :tVent @15:10:02.87 5.5psi after gulp @15:10:02.88 Air stroke #1 of 10 @15:10:02.89 Air.reconfigure :pressurize @15:10:02.98 Air.seek :pressurized @15:10:15.82 7.2psi after pressurize @15:10:15.84 Air.reconfigure :compress @15:10:15.91 Air.seek :evac @15:10:18.58 9.4psi after compress @15:10:55.18 34.8psi after compress (stable) @15:10:55.20 Air.reconfigure :gulp @15:10:55.28 Air.seek :tVent @15:11:05.17 36.8psi after gulp @15:11:05.18 Air stroke #2 of 10 @15:11:05.21 Air.reconfigure :pressurize @15:11:05.29 Air.seek :pressurized @15:11:18.13 33.4psi after pressurize @15:11:18.14 Air.reconfigure :compress @15:11:18.22 Air.seek :evac @15:11:20.88 32.4psi after compress @15:11:50.78 Reached 50.0 of 50.0psi after pumping 4672ul (3.00 strokes) @15:11:50.79 Handoff.select :waste @15:12:16.92 delay 30 seconds @15:12:46.94 Handoff.select :blocked @15:13:11.93 Handoff.select :waste @15:13:12.07 PSI at @2.78psi at evacEnd @15:13:12.08 Handoff.select :blocked @15:13:12.20 Air.reconfigure :gulp @15:13:12.31 Air.seek :tVent @15:13:31.61 Stable @6.1psi after Handoff blocked @15:13:31.69 Air pumping 10 strokes to reach 36psi @15:13:31.70 Air stroke #1 of 10 @15:13:31.72 Air.reconfigure :pressurize @15:13:31.80 Air.seek :pressurized @15:13:44.65 6.5psi after pressurize @15:13:44.66 Air.reconfigure :compress @15:13:44.74 Air.seek :evac @15:13:47.78 6.6psi after compress @15:14:33.74 14.8psi after compress (stable) @15:14:33.76 Air.reconfigure :gulp @15:14:33.84 Air.seek :tVent @15:14:43.76 16.5psi after gulp @15:14:43.77 Air stroke #2 of 10 @15:14:43.80 Air.reconfigure :pressurize @15:14:43.88 Air.seek :pressurized @15:14:56.72 16.9psi after pressurize @15:14:56.73 Air.reconfigure :compress @15:14:56.82 Air.seek :evac @15:14:59.48 16.9psi after compress @15:15:25.93 30.1psi after compress (stable) @15:15:25.95 Air.reconfigure :gulp @15:15:26.03 Air.seek :tVent @15:15:35.92 31.7psi after gulp @15:15:35.93 Air stroke #3 of 10 @15:15:35.95 Air.reconfigure :pressurize @15:15:36.03 Air.seek :pressurized @15:15:48.87 30.9psi after pressurize @15:15:48.88 Air.reconfigure :compress @15:15:48.98 Air.seek :evac @15:15:51.64 30.4psi after compress @15:16:15.61 Reached 36.1 of 36.1psi after pumping 6230ul (4.00 strokes) @15:16:15.62 Handoff.select :waste @15:16:34.05 delay 30 seconds @15:17:04.07 Handoff.select :blocked @15:17:33.86 Handoff.select :waste @15:17:33.97 PSI at @5.29psi at evacEnd @15:17:33.99 Air.reconfigure :default @15:17:34.10 Air.seek :fVent @15:17:35.54 delay 5 seconds @15:17:40.55 Seawater evacuated, Presure above/below filter released. Lysis begins @15:17:40.64 Lysis1.reconfigure :slow @15:17:40.71 Lysis1.seek 1050ul @15:18:10.75 delay 5 seconds @15:18:15.77 Handoff.select :blocked @15:18:15.94 Lysis1.seek 450ul @15:19:15.92 delay 3 seconds @15:19:19.01 Air.reconfigure to mix into 1.37806 parts Lysis1 @15:19:19.47 Air.seek :evac @15:19:19.51 Lysis1.seek 300ul @15:19:34.62 Air.reconfigure :default @15:19:34.68 Air.seek :full @15:19:48.91 Lysis1.seek :empty @15:20:19.02 Lysis Delivered. Syringe pressure @42.1psi @15:20:19.06 PuckHeater.seek 84.1C @15:21:11.14 PuckHeater.hold 15:00,84.1C @15:36:08.71 Lysis complete. Syringe pressure @49.0psi @15:36:08.72 Handoff.select :blocked @15:36:08.82 Lysis1.reconfigure :halfSpeed @15:36:08.89 Lysis1.seek 200ul @15:36:12.66 delay 1 second @15:36:13.68 Lysis1.seek :empty @15:36:17.50 Initial AirS pressure @52.2psi @15:36:17.56 Lysis1.reconfigure :extract @15:36:17.68 Lysis1.seek 900ul,10:00 @15:36:19.39 Air pumping 10 strokes to reach 21psi @15:36:19.40 Air stroke #1 of 10 @15:36:19.42 Air.reconfigure :pressurize @15:36:19.51 Air.seek :pressurized @15:36:32.34 13.5psi after pressurize @15:36:32.35 Air.reconfigure :compress @15:36:32.43 Air.seek :evac @15:36:35.10 16.6psi after compress @15:36:49.01 15.8psi after compress (stable) @15:36:49.03 Air.reconfigure :gulp @15:36:49.10 Air.seek :tVent @15:36:58.99 16.0psi after gulp @15:36:59.00 Air stroke #2 of 10 @15:36:59.02 Air.reconfigure :pressurize @15:36:59.10 Air.seek :pressurized @15:37:11.93 15.7psi after pressurize @15:37:11.94 Air.reconfigure :compress @15:37:12.02 Air.seek :evac @15:37:14.69 16.7psi after compress @15:37:28.60 15.7psi after compress (stable) @15:37:28.63 Air.reconfigure :gulp @15:37:28.70 Air.seek :tVent @15:37:38.59 15.8psi after gulp @15:37:38.60 Air stroke #3 of 10 @15:37:38.62 Air.reconfigure :pressurize @15:37:38.70 Air.seek :pressurized @15:37:51.53 15.5psi after pressurize @15:37:51.54 Air.reconfigure :compress @15:37:51.62 Air.seek :evac @15:37:54.29 15.5psi after compress @15:38:08.19 15.8psi after compress (stable) @15:38:08.20 Air.reconfigure :gulp @15:38:08.30 Air.seek :tVent @15:38:18.18 15.7psi after gulp @15:38:18.19 Air stroke #4 of 10 @15:38:18.21 Air.reconfigure :pressurize @15:38:18.29 Air.seek :pressurized @15:38:31.12 16.0psi after pressurize @15:38:31.13 Air.reconfigure :compress @15:38:31.21 Air.seek :evac @15:38:33.88 15.6psi after compress @15:38:40.08 Air.reconfigure :default @15:38:40.13 Air.hold @15:38:40.30 Lysis1 at 900ul @16.8psi @15:38:40.31 Lysis1.reconfigure :default @15:38:40.39 Lysis1.seek 925ul @15:38:41.18 Lysis1 at 925ul, bottom @11.4psi after recovery @15:38:42.30 Lysis1 at 925ul, bottom @14.1psi @15:38:42.33 Air.reconfigure :halfSpeed @15:38:42.41 Air.seek :fVent @15:38:44.67 Puck Top Vented, bottom @5.7psi @15:38:44.68 Handoff.select :waste @15:38:45.10 delay 5 seconds @15:38:50.21 delay 5 seconds @15:38:55.23 Handoff.select SPR @15:38:55.35 SPR.command \"start 2 14\" @15:38:56.12 delay 10 seconds @15:39:06.14 SPR.command \"start 2 0\" @15:39:09.37 Waiting up to 11:00 for SPR to enter state 1 @15:47:41.45 SPR.command \"hold_on\" @15:47:42.85 SPR Dilution 1 of 1 -- Running Experiment 2 @15:47:42.87 Lysis2.reconfigure :DAmix @15:47:42.98 Lysis1.reconfigure :DAmix @15:47:43.42 Lysis2.seek 1450ul @15:47:43.49 Lysis1.seek 675ul @15:48:18.86 Lysis2.seek 1150ul @15:48:18.89 Lysis1.seek 375ul @15:49:00.91 volL1 = 300.0 and volL2 = 300.0 @15:49:00.93 delay 15 seconds @15:49:15.96 SPR.command \"hold_off\" @15:49:19.00 Waiting up to 25:00 for SPR to enter state 7 @16:00:55.96 SPR Analysis has Completed @16:00:55.97 Handoff.select :waste @16:00:56.88 Ended log /var/log/esp/spr19aug1715h.spr @16:00:56.89 SPR.command \"stats_off\" @16:00:58.53 -> Cmd.stop @16:00:58.66 Handoff.select :close @16:00:58.81 Gate.power :heat,:OFF @16:00:58.83 Gate.power :cartridge,:OFF @16:00:58.91 Carousel.seek 46 @16:00:59.10 Plunge.seek :locked @16:00:59.94 PinPort.seek :clear @16:01:04.57 Archive.seek :clear @16:01:04.70 Air.reconfigure :default @16:01:04.84 Lysis1.reconfigure :default @16:01:04.95 Air.seek :clear @16:01:05.01 Lysis1.seek :clear @16:01:05.09 Lysis2.reconfigure :default @16:01:05.16 Lysis2.seek :clear @16:01:22.32 Archive.coast @16:01:22.37 Air.coast @16:01:22.42 Lysis1.coast @16:01:22.47 Lysis2.coast @16:01:22.53 Plunge.coast @16:01:22.57 SamplePump.coast @16:01:22.62 Toroid.coast @16:01:22.68 Carousel.coast @16:01:22.73 PinPort.coast @16:01:22.91 Gate.power :motor,:OFF @16:01:22.93 Gate.power :core,:OFF @16:01:22.95 Gate.power :analytic1,:OFF @16:01:22.97 Gate.power :analytic2,:OFF @16:01:22.99 Gate.power :analytic3,:OFF @16:01:23.01 Gate.power :main,:OFF @16:01:23.03 Safely stopped and ready to power off ##-- file closed 2019-08-18T11:38:37.230Z