##-- file opened 2018-08-31T04:49:32.019Z @00:48:42.62 -> showStatus @00:48:44.23 -> Cmd.loadCartridge @00:48:44.35 Gate.power :main,:ON,limit to 5000mA @00:48:44.37 Gate.power :analytic1,:ON,limit to 5000mA @00:48:44.39 Gate.power :analytic2,:ON,limit to 5000mA @00:48:44.41 Gate.power :analytic3,:ON,limit to 5000mA @00:48:44.55 Powering core bus @00:48:46.85 SPR Module 1, FW Rev. B, FPGA Rev. C @00:48:46.95 ArS.configure ArSconfig @00:48:47.05 LyS1.configure LyS1config @00:48:47.17 LyS2.configure LyS2config @00:48:47.27 AirS.configure AirSconfig @00:48:47.39 TV.configure TVconfig @00:48:47.51 SP.configure SPconfig @00:48:47.64 SC.configure SCconfig @00:48:47.76 PV.configure PVconfig @00:48:47.88 PinPort.configure PPconfig @00:48:48.00 Sea.configure SPconfig @00:48:48.05 All elf controllers running firmware version 4.5 @00:48:48.08 Gate.power :motor,:ON,limit to 5000mA @00:48:48.20 ArS.reconfigure ArSconfig @00:48:48.41 ArS.home.jog 0 @00:48:48.64 PV.reconfigure PVconfig @00:48:48.72 TV.reconfigure TVconfig @00:48:48.75 LyS2.reconfigure LyS2config @00:48:48.78 PinPort.reconfigure PPconfig @00:48:48.82 LyS1.reconfigure LyS1config @00:48:48.90 AirS.reconfigure AirSconfig @00:48:49.09 PV.home.jog 0 @00:48:49.16 LyS2.home.jog 0 @00:48:49.20 TV.home.jog 0 @00:48:49.22 PinPort.home.jog 0 @00:48:49.26 LyS1.home.jog 0 @00:48:49.30 AirS.home.jog 0 @00:48:49.66 ArS.reconfigure HomeMagSyringe @00:48:49.84 ArS.jog -241 @00:48:50.01 PV.reconfigure HomePV @00:48:50.05 TV.reconfigure HomeTV @00:48:50.08 LyS1.reconfigure HomeSyringe @00:48:50.12 LyS2.reconfigure HomeSyringe @00:48:50.17 PinPort.reconfigure HomePP @00:48:50.20 AirS.reconfigure HomeMagSyringe @00:48:50.35 PV.jog -1475 @00:48:50.40 TV.jog -544 @00:48:50.46 LyS1.jog -263 @00:48:50.53 PinPort.jog -110 @00:48:50.55 AirS.jog -314 @00:48:50.58 LyS2.jog -260 @00:48:50.79 ArS.setRawPosition! -50 @00:48:50.84 ArS.seek :home @00:48:51.17 PinPort.setRawPosition! 0 @00:48:51.23 PinPort.seek :home @00:48:51.35 AirS.setRawPosition! -50 @00:48:51.37 LyS1.setRawPosition! -50 @00:48:51.39 PinPort.reconfigure PPconfig @00:48:51.42 LyS2.setRawPosition! -50 @00:48:51.55 AirS.seek :home @00:48:51.59 LyS1.seek :home @00:48:51.62 LyS2.seek :home @00:48:51.85 PV.setRawPosition! -50 @00:48:51.87 ArS.reconfigure ArSconfig @00:48:51.95 PV.seek :home @00:48:52.13 AirS.reconfigure AirSconfig @00:48:52.16 LyS2.reconfigure LyS2config @00:48:52.19 LyS1.reconfigure LyS1config @00:48:52.38 PV.reconfigure PVconfig @00:48:52.47 TV.setRawPosition! 0 @00:48:52.52 TV.seek :home @00:48:52.61 TV.reconfigure TVconfig @00:48:52.81 TV.seek :clear @00:48:54.44 TV.coast @00:48:54.49 SC.reconfigure HomeSC @00:48:54.55 SC.home.jog 0 @00:48:55.31 SC.reconfigure SCconfig @00:48:55.37 SC.seek 22 @00:48:55.46 Selecting Cartridge 21 @00:48:55.76 SC.seek 21 @00:48:56.26 LyS2.seek :locked @00:48:56.39 PV.seek :locked @00:48:56.52 AirS.seek :locked @00:49:00.60 Powering cartridge bus @00:49:02.72 Gate.setIR 1750,5000,0,6 @00:49:02.85 Gate.setIR 0,5000,0,6 @00:49:02.88 Loaded Archive Cartridge @00:49:02.89 Gate.power :cartridge,:OFF @00:49:22.57 -> Cmd.startFiltering @00:49:22.79 Duration of filtering limited to 1:05:00 @00:49:23.05 Priming sample loop w/100ml, bypass w/10ml @00:49:23.12 SP.setPosition! 0ml @00:49:23.16 Intake.open @00:49:23.19 Exhaust.open @00:49:24.02 Sea @-42.9psia @00:49:24.03 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @00:49:29.81 Vacuum tare is 24% of full scale @00:49:29.83 PB.open @00:49:29.88 Exhaust.close @00:49:30.66 Sea @-42.9psia @00:49:30.74 SP.reconfigure SPprime @00:49:30.81 SP.seek 10ml,1:21 @00:49:41.67 SP.setPosition! 0ml @00:49:41.71 PB.close @00:49:41.74 Exhaust.open @00:49:42.59 Sea @-42.9psia @00:49:42.67 SP.reconfigure SPprime @00:49:42.74 SP.seek 100ml,8:51 @00:51:36.54 Intake.close @00:51:37.30 Pressurizing cartridge to 26psi (try #1 of 3) @00:51:37.31 SP.setPosition! 0ml @00:51:37.36 PB.open @00:51:38.16 TV.seek :crack @00:51:38.96 TV.seek :clear @00:51:39.76 Intake.open @00:51:41.21 PB.close @00:51:41.92 Exhaust.close @00:51:42.71 Sea @-42.9psia @00:51:42.79 SP.reconfigure SPcharge @00:51:42.85 SP.seek 2.3ml,42 seconds @00:51:51.25 Cartridge pressurized with 0.328ml to 26.5psi @00:51:51.48 delay 3 seconds @00:51:54.54 Cartridge at 21.3psi 3 seconds later @00:51:54.62 TV.seek :cartridge @00:51:56.45 TV.seek :clear @00:51:58.26 Sampling 1000.0ml @00:51:58.27 SP.setPosition! 0ml @00:51:58.32 Exhaust.open @00:51:59.08 Sea @-42.9psia @00:51:59.17 SP.reconfigure SPsample @00:51:59.23 SP.seek 1000ml,1:23:53 @00:52:00.19 Cartridge 21 sampling 0/1000ml at 0.17ml/s with 6.1psi vacuum @00:53:00.20 Cartridge 21 sampling 24/1000ml at 0.40ml/s with 26.5psi vacuum @00:54:00.19 Cartridge 21 sampling 48/1000ml at 0.40ml/s with 24.3psi vacuum @00:55:00.20 Cartridge 21 sampling 71/1000ml at 0.35ml/s with 20.1psi vacuum @00:56:00.20 Cartridge 21 sampling 93/1000ml at 0.34ml/s with 19.8psi vacuum @00:57:00.20 Cartridge 21 sampling 114/1000ml at 0.37ml/s with 25.7psi vacuum @00:58:00.20 Cartridge 21 sampling 134/1000ml at 0.34ml/s with 25.2psi vacuum @00:59:00.19 Cartridge 21 sampling 154/1000ml at 0.35ml/s with 20.9psi vacuum @01:00:00.20 Cartridge 21 sampling 173/1000ml at 0.35ml/s with 22.4psi vacuum @01:01:00.20 Cartridge 21 sampling 191/1000ml at 0.31ml/s with 19.9psi vacuum @01:02:00.20 Cartridge 21 sampling 209/1000ml at 0.32ml/s with 22.3psi vacuum @01:03:00.20 Cartridge 21 sampling 226/1000ml at 0.23ml/s with 25.0psi vacuum @01:04:00.20 Cartridge 21 sampling 242/1000ml at 0.25ml/s with 23.6psi vacuum @01:05:00.20 Cartridge 21 sampling 258/1000ml at 0.24ml/s with 22.2psi vacuum @01:06:00.46 Cartridge 21 sampling 274/1000ml at 0.24ml/s with 23.9psi vacuum @01:07:00.20 Cartridge 21 sampling 289/1000ml at 0.24ml/s with 23.3psi vacuum @01:08:00.20 Cartridge 21 sampling 303/1000ml at 0.23ml/s with 20.1psi vacuum @01:09:00.20 Cartridge 21 sampling 317/1000ml at 0.23ml/s with 19.0psi vacuum @01:10:00.20 Cartridge 21 sampling 331/1000ml at 0.20ml/s with 24.4psi vacuum @01:11:00.20 Cartridge 21 sampling 344/1000ml at 0.23ml/s with 20.6psi vacuum @01:12:00.20 Cartridge 21 sampling 357/1000ml at 0.20ml/s with 23.4psi vacuum @01:13:00.20 Cartridge 21 sampling 370/1000ml at 0.20ml/s with 24.3psi vacuum @01:14:00.20 Cartridge 21 sampling 382/1000ml at 0.20ml/s with 26.2psi vacuum @01:15:00.20 Cartridge 21 sampling 395/1000ml at 0.20ml/s with 22.9psi vacuum @01:16:00.20 Cartridge 21 sampling 407/1000ml at 0.20ml/s with 27.8psi vacuum @01:17:00.20 Cartridge 21 sampling 419/1000ml at 0.20ml/s with 28.5psi vacuum @01:18:00.20 Cartridge 21 sampling 431/1000ml at 0.20ml/s with 27.0psi vacuum @01:19:00.20 Cartridge 21 sampling 444/1000ml at 0.20ml/s with 28.7psi vacuum @01:19:27.02 Flow averaged 0.27ml/s over 27:22 @01:19:27.04 Waiting up to 16 seconds for vacuum to reduce from 29.2 to 18.0psi @01:19:27.67 Relieved excess vacuum in 7.4 seconds @01:19:27.69 SP.seek 1000ml,46:26 @01:20:00.20 Cartridge 21 sampling 456/1000ml at 0.20ml/s with 27.6psi vacuum @01:20:23.24 Flow averaged 0.21ml/s over 53 seconds @01:20:23.26 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @01:20:23.88 Relieved excess vacuum in 3.6 seconds @01:20:23.91 SP.seek 1000ml,45:30 @01:20:56.17 Flow averaged 0.28ml/s over 24 seconds @01:20:56.19 Waiting up to 17 seconds for vacuum to reduce from 29.6 to 18.0psi @01:20:56.81 Relieved excess vacuum in 9.8 seconds @01:20:56.83 SP.seek 1000ml,44:58 @01:21:00.19 Cartridge 21 sampling 467/1000ml at 0.20ml/s with 29.9psi vacuum @01:21:06.93 Flow estimate reduced to 0.22ml/s @01:21:06.96 Waiting up to 17 seconds for vacuum to reduce from 29.5 to 18.0psi @01:21:07.57 Relieved excess vacuum in 7.3 seconds @01:21:07.60 SP.seek 1000ml,44:48 @01:21:22.13 Flow averaged 0.22ml/s over 14 seconds @01:21:22.15 Waiting up to 18 seconds for vacuum to reduce from 29.9 to 18.0psi @01:21:22.76 Relieved excess vacuum in 2.1 seconds @01:21:22.79 SP.seek 1000ml,44:34 @01:21:29.91 Flow estimate reduced to 0.17ml/s @01:21:29.93 Intake.close @01:21:29.98 Exhaust.close @01:21:30.76 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @01:21:30.86 Sampled 472.8ml @01:21:30.94 TV.seek :bypass @01:21:32.55 TV.seek :clear @01:21:34.87 -> Cmd.startProcessing @01:21:35.12 PV.seek :reagent @01:21:35.96 Sample loop closed. Ready to Deliver Reagents @01:21:35.97 ArS.seek :sealed @01:21:40.24 ArS.seek :evac @01:21:54.17 delay 20 seconds @01:22:14.20 ArS.seek :clear @01:22:31.91 RNAlater soak for 10:00 @01:32:32.19 ArS.seek :tVent @01:32:34.77 delay 1 second @01:32:35.79 ArS.seek :evac @01:32:51.41 delay 10 seconds @01:33:01.44 Sample preserved and evacuated @01:33:02.64 -> Cmd.stop @01:33:02.78 PV.seek :locked @01:33:03.68 PinPort.seek :clear @01:33:03.82 ArS.seek :clear @01:33:04.00 LyS2.seek :clear @01:33:04.14 AirS.seek :clear @01:33:21.58 ArS.coast @01:33:21.63 LyS1.coast @01:33:21.68 LyS2.coast @01:33:21.73 AirS.coast @01:33:21.78 TV.coast @01:33:21.83 SP.coast @01:33:21.88 SC.coast @01:33:21.93 PV.coast @01:33:21.98 PinPort.coast @01:33:22.17 Gate.power :motor,:OFF @01:33:22.18 Gate.power :main,:OFF @01:33:22.21 Gate.power :core,:OFF @01:33:22.23 Gate.power :analytic1,:OFF @01:33:22.25 Gate.power :analytic2,:OFF @01:33:22.27 Gate.power :analytic3,:OFF @01:33:22.29 Safely stopped and ready to power off ##-- file closed 2018-08-31T05:46:05.255Z ##-- file opened 2018-08-31T05:46:05.262Z @01:45:10.30 -> showStatus @01:45:12.31 -> Cmd.loadCartridge @01:45:12.43 Gate.power :main,:ON,limit to 5000mA @01:45:12.45 Gate.power :analytic1,:ON,limit to 5000mA @01:45:12.47 Gate.power :analytic2,:ON,limit to 5000mA @01:45:12.49 Gate.power :analytic3,:ON,limit to 5000mA @01:45:12.63 Powering core bus @01:45:14.93 SPR Module 1, FW Rev. B, FPGA Rev. C @01:45:15.03 ArS.configure ArSconfig @01:45:15.13 LyS1.configure LyS1config @01:45:15.24 LyS2.configure LyS2config @01:45:15.35 AirS.configure AirSconfig @01:45:15.47 TV.configure TVconfig @01:45:15.58 SP.configure SPconfig @01:45:15.70 SC.configure SCconfig @01:45:15.81 PV.configure PVconfig @01:45:15.93 PinPort.configure PPconfig @01:45:16.05 Sea.configure SPconfig @01:45:16.11 All elf controllers running firmware version 4.5 @01:45:16.13 Gate.power :motor,:ON,limit to 5000mA @01:45:16.38 ArS.reconfigure ArSconfig @01:45:16.43 LyS1.reconfigure LyS1config @01:45:16.65 LyS1.home.jog 0 @01:45:16.67 ArS.home.jog 0 @01:45:16.83 PV.reconfigure PVconfig @01:45:16.90 TV.reconfigure TVconfig @01:45:16.96 LyS2.reconfigure LyS2config @01:45:17.02 PinPort.reconfigure PPconfig @01:45:17.05 AirS.reconfigure AirSconfig @01:45:17.21 TV.home.jog 0 @01:45:17.25 PV.home.jog 0 @01:45:17.28 LyS2.home.jog 0 @01:45:17.31 PinPort.home.jog 0 @01:45:17.33 AirS.home.jog 0 @01:45:17.98 TV.reconfigure HomeTV @01:45:18.00 LyS2.reconfigure HomeSyringe @01:45:18.03 PinPort.reconfigure HomePP @01:45:18.08 PV.reconfigure HomePV @01:45:18.11 AirS.reconfigure HomeMagSyringe @01:45:18.15 ArS.reconfigure HomeMagSyringe @01:45:18.20 LyS1.reconfigure HomeSyringe @01:45:18.38 LyS2.jog -261 @01:45:18.42 TV.jog -545 @01:45:18.45 PV.jog -1477 @01:45:18.48 PinPort.jog -110 @01:45:18.53 AirS.jog -320 @01:45:18.57 LyS1.jog -266 @01:45:18.60 ArS.jog -242 @01:45:19.05 PinPort.setRawPosition! 0 @01:45:19.09 PinPort.seek :home @01:45:19.46 PinPort.reconfigure PPconfig @01:45:19.50 AirS.setRawPosition! -50 @01:45:19.52 LyS2.setRawPosition! -50 @01:45:19.55 ArS.setRawPosition! -50 @01:45:19.58 LyS1.setRawPosition! -50 @01:45:19.70 AirS.seek :home @01:45:19.80 LyS2.seek :home @01:45:19.87 LyS1.seek :home @01:45:19.90 ArS.seek :home @01:45:20.08 AirS.reconfigure AirSconfig @01:45:20.10 PV.setRawPosition! -50 @01:45:20.18 PV.seek :home @01:45:20.36 LyS1.reconfigure LyS1config @01:45:20.39 LyS2.reconfigure LyS2config @01:45:20.43 ArS.reconfigure ArSconfig @01:45:20.62 PV.reconfigure PVconfig @01:45:20.64 TV.setRawPosition! 0 @01:45:20.73 TV.seek :home @01:45:20.82 TV.reconfigure TVconfig @01:45:21.05 TV.seek :clear @01:45:22.68 TV.coast @01:45:22.73 SC.reconfigure HomeSC @01:45:22.80 SC.home.jog 0 @01:45:23.55 SC.reconfigure SCconfig @01:45:23.60 SC.seek 21 @01:45:23.70 Selecting Cartridge 20 @01:45:24.01 SC.seek 20 @01:45:24.52 LyS2.seek :locked @01:45:24.65 PV.seek :locked @01:45:24.77 AirS.seek :locked @01:45:28.85 Powering cartridge bus @01:45:30.98 Gate.setIR 1750,5000,0,6 @01:45:31.11 Gate.setIR 0,5000,0,6 @01:45:31.16 Loaded Lyse-n-Go Cartridge @01:45:31.17 PuckHeater.configure HeatConfig @01:45:31.23 Gate.power :heat,:ON,limit to 5000mA @01:45:51.07 -> Cmd.startFiltering @01:45:51.29 Duration of filtering limited to 1:05:00 @01:45:51.55 Priming sample loop w/100ml, bypass w/10ml @01:45:51.62 SP.setPosition! 0ml @01:45:51.66 Intake.open @01:45:51.68 Exhaust.open @01:45:52.53 Sea @-42.9psia @01:45:52.55 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @01:45:58.33 Vacuum tare is 25% of full scale @01:45:58.35 PB.open @01:45:58.40 Exhaust.close @01:45:59.20 Sea @-42.9psia @01:45:59.28 SP.reconfigure SPprime @01:45:59.35 SP.seek 10ml,1:21 @01:46:10.21 SP.setPosition! 0ml @01:46:10.25 PB.close @01:46:10.28 Exhaust.open @01:46:11.11 Sea @-42.9psia @01:46:11.19 SP.reconfigure SPprime @01:46:11.26 SP.seek 100ml,8:51 @01:48:05.12 Intake.close @01:48:05.89 Pressurizing cartridge to 26psi (try #1 of 3) @01:48:05.90 SP.setPosition! 0ml @01:48:05.95 PB.open @01:48:06.73 TV.seek :crack @01:48:07.52 TV.seek :clear @01:48:08.32 Intake.open @01:48:09.79 PB.close @01:48:10.50 Exhaust.close @01:48:11.27 Sea @-42.9psia @01:48:11.35 SP.reconfigure SPcharge @01:48:11.42 SP.seek 2.3ml,42 seconds @01:48:22.80 Cartridge pressurized with 0.606ml to 26.8psi @01:48:23.03 delay 3 seconds @01:48:26.10 Cartridge at 19.9psi 3 seconds later @01:48:26.18 TV.seek :cartridge @01:48:28.00 TV.seek :clear @01:48:29.81 Sampling 1000.0ml @01:48:29.82 SP.setPosition! 0ml @01:48:29.87 Exhaust.open @01:48:30.63 Sea @-42.9psia @01:48:30.72 SP.reconfigure SPsample @01:48:30.78 SP.seek 1000ml,1:23:53 @01:48:31.74 Cartridge 20 sampling 0/1000ml at 0.17ml/s with 4.2psi vacuum @01:49:31.75 Cartridge 20 sampling 33/1000ml at 0.54ml/s with 26.0psi vacuum @01:50:31.75 Cartridge 20 sampling 65/1000ml at 0.53ml/s with 24.3psi vacuum @01:51:31.75 Cartridge 20 sampling 98/1000ml at 0.52ml/s with 20.4psi vacuum @01:52:31.75 Cartridge 20 sampling 129/1000ml at 0.53ml/s with 21.9psi vacuum @01:53:31.75 Cartridge 20 sampling 161/1000ml at 0.53ml/s with 22.0psi vacuum @01:54:31.75 Cartridge 20 sampling 192/1000ml at 0.51ml/s with 21.5psi vacuum @01:55:31.75 Cartridge 20 sampling 223/1000ml at 0.51ml/s with 24.7psi vacuum @01:56:31.75 Cartridge 20 sampling 255/1000ml at 0.53ml/s with 21.5psi vacuum @01:57:31.75 Cartridge 20 sampling 285/1000ml at 0.49ml/s with 23.5psi vacuum @01:58:31.75 Cartridge 20 sampling 316/1000ml at 0.48ml/s with 24.5psi vacuum @01:59:31.78 Cartridge 20 sampling 346/1000ml at 0.50ml/s with 21.8psi vacuum @02:00:31.75 Cartridge 20 sampling 375/1000ml at 0.49ml/s with 24.8psi vacuum @02:01:31.75 Cartridge 20 sampling 403/1000ml at 0.45ml/s with 25.2psi vacuum @02:02:32.01 Cartridge 20 sampling 431/1000ml at 0.44ml/s with 19.9psi vacuum @02:03:31.75 Cartridge 20 sampling 458/1000ml at 0.45ml/s with 21.3psi vacuum @02:04:31.75 Cartridge 20 sampling 484/1000ml at 0.45ml/s with 20.7psi vacuum @02:05:31.75 Cartridge 20 sampling 509/1000ml at 0.39ml/s with 23.7psi vacuum @02:06:31.75 Cartridge 20 sampling 534/1000ml at 0.41ml/s with 25.9psi vacuum @02:07:31.75 Cartridge 20 sampling 558/1000ml at 0.41ml/s with 24.7psi vacuum @02:08:31.75 Cartridge 20 sampling 580/1000ml at 0.40ml/s with 22.0psi vacuum @02:09:31.76 Cartridge 20 sampling 602/1000ml at 0.33ml/s with 19.1psi vacuum @02:10:31.75 Cartridge 20 sampling 622/1000ml at 0.38ml/s with 22.8psi vacuum @02:11:31.75 Cartridge 20 sampling 642/1000ml at 0.36ml/s with 25.6psi vacuum @02:12:31.75 Cartridge 20 sampling 660/1000ml at 0.32ml/s with 19.4psi vacuum @02:13:31.75 Cartridge 20 sampling 678/1000ml at 0.30ml/s with 25.9psi vacuum @02:14:31.75 Cartridge 20 sampling 695/1000ml at 0.30ml/s with 23.6psi vacuum @02:15:31.75 Cartridge 20 sampling 711/1000ml at 0.28ml/s with 19.5psi vacuum @02:16:31.75 Cartridge 20 sampling 726/1000ml at 0.25ml/s with 24.7psi vacuum @02:17:31.75 Cartridge 20 sampling 741/1000ml at 0.25ml/s with 23.8psi vacuum @02:18:31.75 Cartridge 20 sampling 756/1000ml at 0.23ml/s with 24.5psi vacuum @02:19:31.75 Cartridge 20 sampling 770/1000ml at 0.21ml/s with 23.9psi vacuum @02:20:31.74 Cartridge 20 sampling 783/1000ml at 0.23ml/s with 19.8psi vacuum @02:21:31.75 Cartridge 20 sampling 796/1000ml at 0.22ml/s with 20.9psi vacuum @02:22:31.75 Cartridge 20 sampling 808/1000ml at 0.20ml/s with 22.9psi vacuum @02:23:31.75 Cartridge 20 sampling 821/1000ml at 0.20ml/s with 27.3psi vacuum @02:24:31.75 Cartridge 20 sampling 833/1000ml at 0.20ml/s with 23.0psi vacuum @02:25:31.74 Cartridge 20 sampling 846/1000ml at 0.21ml/s with 21.4psi vacuum @02:26:31.75 Cartridge 20 sampling 858/1000ml at 0.20ml/s with 25.0psi vacuum @02:27:31.75 Cartridge 20 sampling 870/1000ml at 0.20ml/s with 26.1psi vacuum @02:28:24.45 Flow averaged 0.37ml/s over 39:52 @02:28:24.47 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi @02:28:25.10 Relieved excess vacuum in 3.2 seconds @02:28:25.12 SP.seek 1000ml,10:27 @02:28:31.75 Cartridge 20 sampling 882/1000ml at 0.20ml/s with 31.3psi vacuum @02:29:19.40 Flow averaged 0.23ml/s over 47 seconds @02:29:19.42 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi @02:29:20.05 Relieved excess vacuum in 8.1 seconds @02:29:20.07 SP.seek 1000ml,9:33 @02:29:31.74 Flow averaged 0.19ml/s over 12 seconds @02:29:31.75 Intake.close @02:29:31.79 Exhaust.close @02:29:31.95 Cartridge 20 sampling 894/1000ml at 0.00ml/s with 5.5psi vacuum @02:29:32.59 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @02:29:32.69 Sampled 893.8ml @02:29:32.77 TV.seek :bypass @02:29:34.38 TV.seek :clear @02:29:36.67 -> Cmd.startProcessing @02:29:38.72 SPR.command \"read_sequences\" @02:29:39.24 SPR.command \"stats_on\" @02:29:39.28 SPR.command \"start 0 1\" @02:29:41.29 Begun log /var/log/esp/spr18aug3102h.spr @02:29:42.09 Handoff.select :waste @02:29:42.21 ArS.seek empty @02:29:59.91 PinPort.seek :cartridge @02:30:05.62 LyS1.seek 1350ul @02:30:11.56 PV.seek :reagent @02:30:12.39 AirS.seek :tVent @02:30:14.46 delay 2 seconds @02:30:16.49 AirS.reconfigure EvacAirS @02:30:16.54 AirS.seek :evac @02:30:38.84 delay 15 seconds @02:30:53.87 AirS.reconfigure AirSconfig @02:30:53.92 AirS.seek :tVent @02:31:08.13 delay 2 seconds @02:31:10.16 AirS.reconfigure EvacAirS @02:31:10.21 AirS.seek :evac @02:31:32.49 delay 15 seconds @02:31:47.52 AirS.reconfigure AirSconfig @02:31:47.57 AirS.seek :tVent @02:32:01.78 delay 2 seconds @02:32:03.81 AirS.reconfigure EvacAirS @02:32:03.87 AirS.seek :evac @02:32:26.15 delay 15 seconds @02:32:41.18 AirS.reconfigure AirSconfig @02:32:41.23 AirS.seek :tVent @02:32:55.43 delay 2 seconds @02:32:57.46 AirS.reconfigure EvacAirS @02:32:57.51 AirS.seek :evac @02:33:19.79 delay 15 seconds @02:33:34.82 AirS.reconfigure AirSconfig @02:33:34.87 AirS.seek :tVent @02:33:49.07 delay 2 seconds @02:33:51.10 AirS.reconfigure EvacAirS @02:33:51.15 AirS.seek :evac @02:34:13.44 delay 15 seconds @02:34:28.46 delay 1:30 @02:35:58.53 AirS.reconfigure AirSconfig @02:35:58.59 AirS.seek :fVent @02:36:00.03 delay 15 seconds @02:36:15.05 LyS1.reconfigure SlowLyS1 @02:36:15.17 LyS1.seek 1050ul @02:36:45.18 delay 5 seconds @02:36:50.20 Handoff.select :blocked @02:36:50.36 LyS1.seek 450ul @02:37:50.31 delay 10 seconds @02:38:00.33 AirS.reconfigure SlowAirS @02:38:00.45 AirS.reconfigure to mix into 1.37806 parts LyS1 @02:38:00.83 AirS.seek evac @02:38:00.87 LyS1.seek 300ul @02:38:15.97 AirS.reconfigure AirSconfig @02:38:16.03 AirS.seek :full @02:38:30.24 LyS1.seek :empty @02:39:00.33 PuckHeater.seek 84.1C @02:40:01.65 PuckHeater.hold 15:00,84.1C @02:54:58.30 LyS1.reconfigure LyS1config @02:54:58.36 LyS1.seek 900ul @02:55:06.70 AirS.seek :tVent @02:55:06.78 delay 2 seconds @02:55:08.81 AirS.reconfigure HalfSpeedAirS @02:55:08.87 AirS.seek :evac @02:55:36.81 delay 15 seconds @02:55:51.89 AirS.reconfigure AirSconfig @02:55:51.94 AirS.seek :tVent @02:56:06.16 delay 2 seconds @02:56:08.19 AirS.reconfigure HalfSpeedAirS @02:56:08.24 AirS.seek :evac @02:56:35.97 delay 15 seconds @02:56:51.00 AirS.reconfigure AirSconfig @02:56:51.05 AirS.seek :tVent @02:57:05.27 delay 2 seconds @02:57:07.30 AirS.reconfigure HalfSpeedAirS @02:57:07.35 AirS.seek :evac @02:57:35.12 delay 15 seconds @02:57:50.13 delay 1:00 @02:58:50.15 Handoff.select :waste @02:58:50.25 delay 15 seconds @02:59:05.28 AirS.seek :fVent @02:59:07.47 delay 10 seconds @02:59:17.50 AirS.reconfigure AirSconfig @02:59:17.57 AirS.seek :evac @02:59:19.01 Lysis Recovered in LyS1 @02:59:19.02 Lysis Complete @02:59:19.03 Handoff.select :waste @02:59:19.05 LyS2.seek :full @02:59:19.14 LyS1.reconfigure DAmixLyS1 @02:59:19.19 LyS2.reconfigure DAmixLyS2 @02:59:19.59 LyS2.seek 1480ul @02:59:19.62 LyS1.seek 650ul @03:00:06.95 delay 5 seconds @03:00:11.96 Handoff.select :SPR @03:00:12.07 SPR.command \"start 2 14\" @03:00:13.21 Running Experiment 2 @03:00:13.22 delay 10 seconds @03:00:23.24 SPR.command \"start 2 0\" @03:00:27.08 Waiting up to 11:00 for SPR to enter state 1 @03:07:45.82 SPR.command \"hold_on\" @03:07:48.00 LyS2.seek 1130ul @03:07:48.03 LyS1.seek 300ul @03:08:54.21 delay 15 seconds @03:09:09.23 SPR.command \"hold_off\" @03:09:12.97 Waiting up to 25:00 for SPR to enter state 7 @03:20:48.96 Handoff.select :waste @03:20:49.08 LyS1.reconfigure LyS1config @03:20:49.14 LyS2.reconfigure LyS2config @03:20:49.21 Rep #1 SPR Analysis has Completed @03:20:49.89 Ended log /var/log/esp/spr18aug3102h.spr @03:20:49.90 SPR.command \"stats_off\" @03:20:52.02 -> Cmd.stop @03:20:52.11 Handoff.select :close @03:20:52.28 Gate.power :heat,:OFF @03:20:52.29 Gate.power :cartridge,:OFF @03:20:52.32 PV.seek :locked @03:20:53.21 PinPort.seek :clear @03:20:59.22 ArS.seek :clear @03:20:59.35 LyS1.seek :clear @03:20:59.48 LyS2.seek :clear @03:20:59.61 AirS.seek :clear @03:21:16.97 ArS.coast @03:21:17.02 LyS1.coast @03:21:17.07 LyS2.coast @03:21:17.11 AirS.coast @03:21:17.16 TV.coast @03:21:17.21 SP.coast @03:21:17.26 SC.coast @03:21:17.32 PV.coast @03:21:17.37 PinPort.coast @03:21:17.54 Gate.power :motor,:OFF @03:21:17.56 Gate.power :main,:OFF @03:21:17.58 Gate.power :core,:OFF @03:21:17.60 Gate.power :analytic1,:OFF @03:21:17.62 Gate.power :analytic2,:OFF @03:21:17.64 Gate.power :analytic3,:OFF @03:21:17.66 Safely stopped and ready to power off ##-- file closed 2018-08-31T07:33:18.901Z ##-- file opened 2018-08-31T07:33:18.903Z @03:32:14.14 -> showStatus @03:32:16.12 -> Cmd.loadCartridge @03:32:16.24 Gate.power :main,:ON,limit to 5000mA @03:32:16.26 Gate.power :analytic1,:ON,limit to 5000mA @03:32:16.28 Gate.power :analytic2,:ON,limit to 5000mA @03:32:16.30 Gate.power :analytic3,:ON,limit to 5000mA @03:32:16.45 Powering core bus @03:32:18.75 SPR Module 1, FW Rev. B, FPGA Rev. C @03:32:18.84 ArS.configure ArSconfig @03:32:18.94 LyS1.configure LyS1config @03:32:19.06 LyS2.configure LyS2config @03:32:19.18 AirS.configure AirSconfig @03:32:19.29 TV.configure TVconfig @03:32:19.41 SP.configure SPconfig @03:32:19.53 SC.configure SCconfig @03:32:19.64 PV.configure PVconfig @03:32:19.76 PinPort.configure PPconfig @03:32:19.88 Sea.configure SPconfig @03:32:19.94 All elf controllers running firmware version 4.5 @03:32:19.96 Gate.power :motor,:ON,limit to 5000mA @03:32:20.32 LyS1.reconfigure LyS1config @03:32:20.37 LyS2.reconfigure LyS2config @03:32:20.42 ArS.reconfigure ArSconfig @03:32:20.53 LyS2.home.jog 0 @03:32:20.56 LyS1.home.jog 0 @03:32:20.59 ArS.home.jog 0 @03:32:20.74 PV.reconfigure PVconfig @03:32:20.80 TV.reconfigure TVconfig @03:32:20.83 PinPort.reconfigure PPconfig @03:32:20.88 AirS.reconfigure AirSconfig @03:32:20.99 PV.home.jog 0 @03:32:21.04 TV.home.jog 0 @03:32:21.08 PinPort.home.jog 0 @03:32:21.11 AirS.home.jog 0 @03:32:21.75 AirS.reconfigure HomeMagSyringe @03:32:21.79 LyS2.reconfigure HomeSyringe @03:32:21.82 LyS1.reconfigure HomeSyringe @03:32:21.87 PV.reconfigure HomePV @03:32:21.93 TV.reconfigure HomeTV @03:32:21.99 ArS.reconfigure HomeMagSyringe @03:32:22.02 PinPort.reconfigure HomePP @03:32:22.23 AirS.jog -326 @03:32:22.28 PV.jog -1478 @03:32:22.31 LyS1.jog -262 @03:32:22.34 LyS2.jog -259 @03:32:22.37 ArS.jog -256 @03:32:22.39 TV.jog -548 @03:32:22.45 PinPort.jog -110 @03:32:22.97 PinPort.setRawPosition! 0 @03:32:23.24 PinPort.seek :home @03:32:23.39 AirS.setRawPosition! -50 @03:32:23.42 LyS1.setRawPosition! -50 @03:32:23.44 ArS.setRawPosition! -50 @03:32:23.46 PinPort.reconfigure PPconfig @03:32:23.49 LyS2.setRawPosition! -50 @03:32:23.63 AirS.seek :home @03:32:23.71 LyS1.seek :home @03:32:23.74 LyS2.seek :home @03:32:23.77 ArS.seek :home @03:32:23.98 PV.setRawPosition! -50 @03:32:24.03 PV.seek :home @03:32:24.13 AirS.reconfigure AirSconfig @03:32:24.26 LyS1.reconfigure LyS1config @03:32:24.30 ArS.reconfigure ArSconfig @03:32:24.33 LyS2.reconfigure LyS2config @03:32:24.51 PV.reconfigure PVconfig @03:32:24.53 TV.setRawPosition! 0 @03:32:24.62 TV.seek :home @03:32:24.71 TV.reconfigure TVconfig @03:32:24.94 TV.seek :clear @03:32:26.56 TV.coast @03:32:26.61 SC.reconfigure HomeSC @03:32:26.68 SC.home.jog 0 @03:32:27.43 SC.reconfigure SCconfig @03:32:27.48 SC.seek 20 @03:32:27.57 Selecting Cartridge 19 @03:32:27.88 SC.seek 19 @03:32:28.38 LyS2.seek :locked @03:32:28.51 PV.seek :locked @03:32:28.64 AirS.seek :locked @03:32:32.72 Powering cartridge bus @03:32:34.85 Gate.setIR 1750,5000,0,6 @03:32:34.97 Gate.setIR 0,5000,0,6 @03:32:35.01 Loaded Archive Cartridge Gate.power :cartridge,:OFF @03:32:54.45 -> Cmd.startFiltering @03:32:54.67 Duration of filtering limited to 1:05:00 @03:32:54.93 Priming sample loop w/100ml, bypass w/10ml @03:32:55.00 SP.setPosition! 0ml @03:32:55.04 Intake.open @03:32:55.08 Exhaust.open @03:32:55.88 Sea @-42.9psia @03:32:55.89 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @03:33:01.67 Vacuum tare is 25% of full scale @03:33:01.69 PB.open @03:33:01.72 Exhaust.close @03:33:02.51 Sea @-42.9psia @03:33:02.60 SP.reconfigure SPprime @03:33:02.66 SP.seek 10ml,1:21 @03:33:13.52 SP.setPosition! 0ml @03:33:13.56 PB.close @03:33:13.59 Exhaust.open @03:33:14.41 Sea @-42.9psia @03:33:14.49 SP.reconfigure SPprime @03:33:14.55 SP.seek 100ml,8:51 @03:35:08.21 Intake.close @03:35:08.98 Pressurizing cartridge to 26psi (try #1 of 3) @03:35:08.99 SP.setPosition! 0ml @03:35:09.04 PB.open @03:35:09.86 TV.seek :crack @03:35:10.65 TV.seek :clear @03:35:11.45 Intake.open @03:35:12.92 PB.close @03:35:13.65 Exhaust.close @03:35:14.42 Sea @-42.9psia @03:35:14.50 SP.reconfigure SPcharge @03:35:14.57 SP.seek 2.3ml,42 seconds @03:35:22.31 Cartridge pressurized with 0.278ml to 26.4psi @03:35:22.54 delay 3 seconds @03:35:25.61 Cartridge at 20.3psi 3 seconds later @03:35:25.68 TV.seek :cartridge @03:35:27.50 TV.seek :clear @03:35:29.32 Sampling 1000.0ml @03:35:29.33 SP.setPosition! 0ml @03:35:29.38 Exhaust.open @03:35:30.13 Sea @-42.9psia @03:35:30.22 SP.reconfigure SPsample @03:35:30.28 SP.seek 1000ml,1:23:53 @03:35:31.24 Cartridge 19 sampling 0/1000ml at 0.17ml/s with 6.3psi vacuum @03:36:31.25 Cartridge 19 sampling 24/1000ml at 0.41ml/s with 24.4psi vacuum @03:37:31.24 Cartridge 19 sampling 48/1000ml at 0.35ml/s with 23.5psi vacuum @03:38:31.25 Cartridge 19 sampling 70/1000ml at 0.41ml/s with 23.7psi vacuum @03:39:31.25 Cartridge 19 sampling 92/1000ml at 0.33ml/s with 20.0psi vacuum @03:40:31.25 Cartridge 19 sampling 112/1000ml at 0.30ml/s with 21.6psi vacuum @03:41:31.25 Cartridge 19 sampling 132/1000ml at 0.30ml/s with 18.6psi vacuum @03:42:31.24 Cartridge 19 sampling 151/1000ml at 0.34ml/s with 21.4psi vacuum @03:43:31.25 Cartridge 19 sampling 169/1000ml at 0.31ml/s with 19.8psi vacuum @03:44:31.25 Cartridge 19 sampling 187/1000ml at 0.26ml/s with 20.6psi vacuum @03:45:31.25 Cartridge 19 sampling 204/1000ml at 0.24ml/s with 22.3psi vacuum @03:46:31.25 Cartridge 19 sampling 220/1000ml at 0.22ml/s with 23.7psi vacuum @03:47:31.24 Cartridge 19 sampling 236/1000ml at 0.22ml/s with 22.3psi vacuum @03:48:31.25 Cartridge 19 sampling 251/1000ml at 0.26ml/s with 19.6psi vacuum @03:49:31.51 Cartridge 19 sampling 265/1000ml at 0.23ml/s with 22.9psi vacuum @03:50:31.25 Cartridge 19 sampling 279/1000ml at 0.22ml/s with 22.3psi vacuum @03:51:31.25 Cartridge 19 sampling 293/1000ml at 0.20ml/s with 23.1psi vacuum @03:52:31.25 Cartridge 19 sampling 306/1000ml at 0.24ml/s with 20.3psi vacuum @03:53:31.25 Cartridge 19 sampling 319/1000ml at 0.20ml/s with 24.3psi vacuum @03:54:31.25 Cartridge 19 sampling 331/1000ml at 0.20ml/s with 24.8psi vacuum @03:55:31.25 Cartridge 19 sampling 344/1000ml at 0.20ml/s with 27.5psi vacuum @03:56:31.25 Cartridge 19 sampling 356/1000ml at 0.20ml/s with 22.7psi vacuum @03:57:31.25 Cartridge 19 sampling 368/1000ml at 0.20ml/s with 24.0psi vacuum @03:58:31.25 Cartridge 19 sampling 380/1000ml at 0.20ml/s with 24.6psi vacuum @03:59:26.23 Flow averaged 0.27ml/s over 23:52 @03:59:26.25 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi @03:59:26.87 Relieved excess vacuum in 5.4 seconds @03:59:26.89 SP.seek 1000ml,51:15 @03:59:31.25 Cartridge 19 sampling 392/1000ml at 0.20ml/s with 24.9psi vacuum @04:00:31.19 Flow averaged 0.23ml/s over 55 seconds @04:00:31.21 Waiting up to 17 seconds for vacuum to reduce from 29.8 to 18.0psi @04:00:31.34 Cartridge 19 sampling 404/1000ml at 0.00ml/s with 14.7psi vacuum @04:00:31.84 Relieved excess vacuum in 10.4 seconds @04:00:31.86 SP.seek 1000ml,50:11 @04:00:49.70 Flow averaged 0.27ml/s over 14 seconds @04:00:49.72 Waiting up to 19 seconds for vacuum to reduce from 30.6 to 18.0psi @04:00:50.34 Relieved excess vacuum in 5.4 seconds @04:00:50.36 SP.seek 1000ml,49:54 @04:01:00.64 Flow estimate reduced to 0.21ml/s @04:01:00.67 Waiting up to 15 seconds for vacuum to reduce from 28.8 to 18.0psi @04:01:01.29 Relieved excess vacuum in 7.8 seconds @04:01:01.31 SP.seek 1000ml,49:44 @04:01:16.23 Flow averaged 0.22ml/s over 14 seconds @04:01:16.25 Waiting up to 15 seconds for vacuum to reduce from 28.9 to 18.0psi @04:01:16.88 Relieved excess vacuum in 2.5 seconds @04:01:16.90 SP.seek 1000ml,49:29 @04:01:22.28 Flow estimate reduced to 0.18ml/s @04:01:22.30 Intake.close @04:01:22.35 Exhaust.close @04:01:23.13 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @04:01:23.23 Sampled 413.4ml @04:01:23.31 TV.seek :bypass @04:01:24.92 TV.seek :clear @04:01:27.11 -> Cmd.startProcessing @04:01:27.36 PV.seek :reagent @04:01:28.21 Sample loop closed. Ready to Deliver Reagents @04:01:28.22 ArS.seek :sealed @04:01:32.50 ArS.seek :evac @04:01:46.43 delay 20 seconds @04:02:06.46 ArS.seek :clear @04:02:24.17 RNAlater soak for 10:00 @04:12:24.20 ArS.seek :tVent @04:12:26.77 delay 1 second @04:12:27.80 ArS.seek :evac @04:12:43.42 delay 10 seconds @04:12:53.45 Sample preserved and evacuated @04:12:54.43 -> Cmd.stop @04:12:54.57 PV.seek :locked @04:12:55.45 PinPort.seek :clear @04:12:55.84 ArS.seek :clear @04:12:56.03 LyS2.seek :clear @04:12:56.17 AirS.seek :clear @04:13:13.60 ArS.coast @04:13:13.65 LyS1.coast @04:13:13.69 LyS2.coast @04:13:13.74 AirS.coast @04:13:13.79 TV.coast @04:13:13.85 SP.coast @04:13:13.90 SC.coast @04:13:13.95 PV.coast @04:13:14.00 PinPort.coast @04:13:14.17 Gate.power :motor,:OFF @04:13:14.19 Gate.power :main,:OFF @04:13:14.21 Gate.power :core,:OFF @04:13:14.23 Gate.power :analytic1,:OFF @04:13:14.25 Gate.power :analytic2,:OFF @04:13:14.27 Gate.power :analytic3,:OFF @04:13:14.29 Safely stopped and ready to power off ##-- file closed 2018-08-31T09:42:34.570Z ##-- file opened 2018-08-31T09:42:34.581Z @05:41:17.79 -> showStatus @05:41:19.78 -> Cmd.loadCartridge @05:41:19.91 Gate.power :main,:ON,limit to 5000mA @05:41:19.92 Gate.power :analytic1,:ON,limit to 5000mA @05:41:19.95 Gate.power :analytic2,:ON,limit to 5000mA @05:41:19.97 Gate.power :analytic3,:ON,limit to 5000mA @05:41:20.12 Powering core bus @05:41:22.41 SPR Module 1, FW Rev. B, FPGA Rev. C @05:41:22.50 ArS.configure ArSconfig @05:41:22.60 LyS1.configure LyS1config @05:41:22.72 LyS2.configure LyS2config @05:41:22.84 AirS.configure AirSconfig @05:41:22.96 TV.configure TVconfig @05:41:23.08 SP.configure SPconfig @05:41:23.20 SC.configure SCconfig @05:41:23.32 PV.configure PVconfig @05:41:23.44 PinPort.configure PPconfig @05:41:23.55 Sea.configure SPconfig @05:41:23.61 All elf controllers running firmware version 4.5 @05:41:23.63 Gate.power :motor,:ON,limit to 5000mA @05:41:24.17 PV.reconfigure PVconfig @05:41:24.20 TV.reconfigure TVconfig @05:41:24.23 AirS.reconfigure AirSconfig @05:41:24.27 LyS1.reconfigure LyS1config @05:41:24.31 PinPort.reconfigure PPconfig @05:41:24.34 ArS.reconfigure ArSconfig @05:41:24.39 LyS2.reconfigure LyS2config @05:41:24.59 PV.home.jog 0 @05:41:24.62 TV.home.jog 0 @05:41:24.66 LyS1.home.jog 0 @05:41:24.68 PinPort.home.jog 0 @05:41:24.70 AirS.home.jog 0 @05:41:24.73 ArS.home.jog 0 @05:41:24.76 LyS2.home.jog 0 @05:41:25.40 TV.reconfigure HomeTV @05:41:25.45 LyS1.reconfigure HomeSyringe @05:41:25.50 PV.reconfigure HomePV @05:41:25.55 PinPort.reconfigure HomePP @05:41:25.71 TV.jog -545 @05:41:25.76 LyS1.jog -259 @05:41:25.80 PinPort.jog -110 @05:41:25.82 PV.jog -1474 @05:41:26.05 LyS2.reconfigure HomeSyringe @05:41:26.08 ArS.reconfigure HomeMagSyringe @05:41:26.15 AirS.reconfigure HomeMagSyringe @05:41:26.21 LyS2.jog -258 @05:41:26.32 AirS.jog -315 @05:41:26.35 ArS.jog -244 @05:41:26.72 PinPort.setRawPosition! 0 @05:41:26.73 LyS1.setRawPosition! -50 @05:41:26.83 LyS1.seek :home @05:41:26.86 PinPort.seek :home @05:41:27.01 PinPort.reconfigure PPconfig @05:41:27.02 AirS.setRawPosition! -50 @05:41:27.05 LyS2.setRawPosition! -50 @05:41:27.18 AirS.seek :home @05:41:27.22 LyS2.seek :home @05:41:27.35 LyS1.reconfigure LyS1config @05:41:27.37 ArS.setRawPosition! -50 @05:41:27.40 PV.setRawPosition! -50 @05:41:27.50 ArS.seek :home @05:41:27.58 PV.seek :home @05:41:27.73 LyS2.reconfigure LyS2config @05:41:27.76 AirS.reconfigure AirSconfig @05:41:27.93 ArS.reconfigure ArSconfig @05:41:27.95 TV.setRawPosition! 0 @05:41:28.04 TV.seek :home @05:41:28.15 PV.reconfigure PVconfig @05:41:28.17 TV.reconfigure TVconfig @05:41:28.52 TV.seek :clear @05:41:30.14 TV.coast @05:41:30.20 SC.reconfigure HomeSC @05:41:30.25 SC.home.jog 0 @05:41:31.01 SC.reconfigure SCconfig @05:41:31.07 SC.seek 19 @05:41:31.16 Selecting Cartridge 18 @05:41:31.46 SC.seek 18 @05:41:31.96 LyS2.seek :locked @05:41:32.09 PV.seek :locked @05:41:32.22 AirS.seek :locked @05:41:36.30 Powering cartridge bus @05:41:38.43 Gate.setIR 1750,5000,0,6 @05:41:38.56 Gate.setIR 0,5000,0,6 @05:41:38.59 Loaded Archive Cartridge @05:41:38.60 Gate.power :cartridge,:OFF @05:41:58.14 -> Cmd.startFiltering @05:41:58.37 Duration of filtering limited to 1:05:00 @05:41:58.61 Priming sample loop w/100ml, bypass w/10ml @05:41:58.69 SP.setPosition! 0ml @05:41:58.73 Intake.open @05:41:58.76 Exhaust.open @05:41:59.59 Sea @-42.9psia @05:41:59.61 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @05:42:05.39 Vacuum tare is 24% of full scale @05:42:05.41 PB.open @05:42:05.45 Exhaust.close @05:42:06.25 Sea @-42.9psia @05:42:06.33 SP.reconfigure SPprime @05:42:06.39 SP.seek 10ml,1:21 @05:42:17.25 SP.setPosition! 0ml @05:42:17.30 PB.close @05:42:17.33 Exhaust.open @05:42:18.16 Sea @-42.9psia @05:42:18.24 SP.reconfigure SPprime @05:42:18.31 SP.seek 100ml,8:51 @05:44:11.99 Intake.close @05:44:12.71 Pressurizing cartridge to 26psi (try #1 of 3) @05:44:12.73 SP.setPosition! 0ml @05:44:12.78 PB.open @05:44:13.58 TV.seek :crack @05:44:14.37 TV.seek :clear @05:44:15.16 Intake.open @05:44:16.63 PB.close @05:44:17.34 Exhaust.close @05:44:18.11 Sea @-42.9psia @05:44:18.20 SP.reconfigure SPcharge @05:44:18.26 SP.seek 2.3ml,42 seconds @05:44:26.16 Cartridge pressurized with 0.290ml to 27.9psi @05:44:26.39 delay 3 seconds @05:44:29.46 Cartridge at 22.3psi 3 seconds later @05:44:29.54 TV.seek :cartridge @05:44:31.37 TV.seek :clear @05:44:33.18 Sampling 1000.0ml @05:44:33.19 SP.setPosition! 0ml @05:44:33.24 Exhaust.open @05:44:34.00 Sea @-42.9psia @05:44:34.08 SP.reconfigure SPsample @05:44:34.15 SP.seek 1000ml,1:23:53 @05:44:35.11 Cartridge 18 sampling 0/1000ml at 0.17ml/s with 5.7psi vacuum @05:45:35.12 Cartridge 18 sampling 22/1000ml at 0.38ml/s with 24.0psi vacuum @05:46:35.11 Cartridge 18 sampling 40/1000ml at 0.29ml/s with 19.5psi vacuum @05:47:35.11 Cartridge 18 sampling 55/1000ml at 0.20ml/s with 24.4psi vacuum @05:48:25.19 Flow averaged 0.29ml/s over 3:42 @05:48:25.22 Waiting up to 17 seconds for vacuum to reduce from 29.6 to 18.0psi @05:48:25.83 Relieved excess vacuum in 10.6 seconds @05:48:25.86 SP.seek 1000ml,1:18:28 @05:48:31.21 Flow estimate reduced to 0.23ml/s @05:48:31.24 Waiting up to 17 seconds for vacuum to reduce from 29.8 to 18.0psi @05:48:31.85 Relieved excess vacuum in 2.9 seconds @05:48:31.88 SP.seek 1000ml,1:18:23 @05:48:35.10 Cartridge 18 sampling 66/1000ml at 0.20ml/s with 29.7psi vacuum @05:48:35.42 Flow estimate reduced to 0.19ml/s @05:48:35.44 Intake.close @05:48:35.48 Exhaust.close @05:48:36.30 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @05:48:36.40 Sampled 66.5ml @05:48:36.48 TV.seek :bypass @05:48:38.09 TV.seek :clear @05:48:40.37 -> Cmd.startProcessing @05:48:40.87 PV.seek :reagent @05:48:41.71 Sample loop closed. Ready to Deliver Reagents @05:48:41.73 ArS.seek :sealed @05:48:46.00 ArS.seek :evac @05:48:59.93 delay 20 seconds @05:49:19.97 ArS.seek :clear @05:49:37.69 RNAlater soak for 10:00 @05:59:37.71 ArS.seek :tVent @05:59:40.28 delay 1 second @05:59:41.31 ArS.seek :evac @05:59:56.92 delay 10 seconds @06:00:06.96 Sample preserved and evacuated @06:00:08.10 -> Cmd.stop @06:00:08.25 PV.seek :locked @06:00:09.14 PinPort.seek :clear @06:00:09.28 ArS.seek :clear @06:00:09.47 LyS2.seek :clear @06:00:09.61 AirS.seek :clear @06:00:27.03 ArS.coast @06:00:27.08 LyS1.coast @06:00:27.13 LyS2.coast @06:00:27.18 AirS.coast @06:00:27.23 TV.coast @06:00:27.27 SP.coast @06:00:27.32 SC.coast @06:00:27.37 PV.coast @06:00:27.42 PinPort.coast @06:00:27.60 Gate.power :motor,:OFF @06:00:27.61 Gate.power :main,:OFF @06:00:27.63 Gate.power :core,:OFF @06:00:27.66 Gate.power :analytic1,:OFF @06:00:27.68 Gate.power :analytic2,:OFF @06:00:27.70 Gate.power :analytic3,:OFF @06:00:27.72 Safely stopped and ready to power off ##-- file closed 2018-08-31T17:19:15.363Z