##-- file opened 2018-08-26T19:21:53.835Z @15:21:31.75 -> showStatus @15:21:33.32 -> Cmd.loadCartridge @15:21:33.44 Gate.power :main,:ON,limit to 5000mA @15:21:33.45 Gate.power :analytic1,:ON,limit to 5000mA @15:21:33.47 Gate.power :analytic2,:ON,limit to 5000mA @15:21:33.49 Gate.power :analytic3,:ON,limit to 5000mA @15:21:33.63 Powering core bus @15:21:35.93 SPR Module 1, FW Rev. B, FPGA Rev. C @15:21:36.03 ArS.configure ArSconfig @15:21:36.13 LyS1.configure LyS1config @15:21:36.25 LyS2.configure LyS2config @15:21:36.35 AirS.configure AirSconfig @15:21:36.47 TV.configure TVconfig @15:21:36.58 SP.configure SPconfig @15:21:36.70 SC.configure SCconfig @15:21:36.83 PV.configure PVconfig @15:21:36.94 PinPort.configure PPconfig @15:21:37.06 Sea.configure SPconfig @15:21:37.12 All elf controllers running firmware version 4.5 @15:21:37.13 Gate.power :motor,:ON,limit to 5000mA @15:21:37.27 ArS.reconfigure ArSconfig @15:21:37.53 ArS.home.jog 0 @15:21:37.68 PV.reconfigure PVconfig @15:21:37.75 TV.reconfigure TVconfig @15:21:37.79 LyS2.reconfigure LyS2config @15:21:37.82 LyS1.reconfigure LyS1config @15:21:37.86 PinPort.reconfigure PPconfig @15:21:37.95 AirS.reconfigure AirSconfig @15:21:38.06 PV.home.jog 0 @15:21:38.14 TV.home.jog 0 @15:21:38.17 LyS2.home.jog 0 @15:21:38.20 PinPort.home.jog 0 @15:21:38.26 LyS1.home.jog 0 @15:21:38.30 AirS.home.jog 0 @15:21:38.57 ArS.reconfigure HomeMagSyringe @15:21:39.00 PinPort.reconfigure HomePP @15:21:39.02 PV.reconfigure HomePV @15:21:39.05 TV.reconfigure HomeTV @15:21:39.09 LyS2.reconfigure HomeSyringe @15:21:39.12 ArS.jog -8650 @15:21:39.29 PV.jog -2166 @15:21:39.33 PinPort.jog -2410 @15:21:39.36 LyS2.jog -2502 @15:21:39.40 TV.jog -554 @15:21:39.61 LyS1.reconfigure HomeSyringe @15:21:39.63 AirS.reconfigure HomeMagSyringe @15:21:39.73 AirS.jog -7964 @15:21:39.76 LyS1.jog -5976 @15:21:40.88 PV.setRawPosition! -50 @15:21:40.92 PV.seek :home @15:21:41.09 LyS2.setRawPosition! -50 @15:21:41.13 LyS2.seek :home @15:21:41.22 PV.reconfigure PVconfig @15:21:41.54 TV.setRawPosition! 0 @15:21:41.56 LyS2.reconfigure LyS2config @15:21:41.64 TV.seek :home @15:21:41.75 TV.reconfigure TVconfig @15:21:42.81 LyS1.setRawPosition! -50 @15:21:42.85 LyS1.seek :home @15:21:43.13 LyS1.reconfigure LyS1config @15:21:43.39 ArS.setRawPosition! -50 @15:21:43.43 ArS.seek :home @15:21:43.61 AirS.setRawPosition! -50 @15:21:43.65 AirS.seek :home @15:21:43.74 ArS.reconfigure ArSconfig @15:21:43.94 AirS.reconfigure AirSconfig @15:21:45.17 PinPort.setRawPosition! 0 @15:21:45.22 PinPort.seek :home @15:21:45.30 PinPort.reconfigure PPconfig @15:21:45.83 TV.seek :clear @15:21:47.43 TV.coast @15:21:47.48 SC.reconfigure HomeSC @15:21:47.54 SC.home.jog 0 @15:21:48.29 SC.reconfigure SCconfig @15:21:48.35 SC.seek 46 @15:21:48.45 Selecting Cartridge 45 @15:21:48.76 SC.seek 45 @15:21:49.25 LyS2.seek :locked @15:21:49.38 PV.seek :locked @15:21:49.50 AirS.seek :locked @15:21:53.58 Powering cartridge bus @15:21:55.70 Gate.setIR 1750,5000,0,6 @15:21:55.83 Gate.setIR 0,5000,0,6 @15:21:55.86 Loaded Archive Cartridge @15:21:55.87 Gate.power :cartridge,:OFF @15:22:11.66 -> Cmd.startFiltering @15:22:11.88 Duration of filtering limited to 1:05:00 @15:22:12.14 Priming sample loop w/100ml, bypass w/10ml @15:22:12.22 SP.setPosition! 0ml @15:22:12.26 Intake.open @15:22:12.32 Exhaust.open @15:22:13.12 Sea @-42.9psia @15:22:13.13 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @15:22:18.66 Vacuum tare is 24% of full scale @15:22:18.67 PB.open @15:22:18.71 Exhaust.close @15:22:19.56 Sea @-42.9psia @15:22:19.64 SP.reconfigure SPprime @15:22:19.70 SP.seek 10ml,1:21 @15:22:30.56 SP.setPosition! 0ml @15:22:30.60 PB.close @15:22:30.88 Exhaust.open @15:22:31.72 Sea @-42.9psia @15:22:31.80 SP.reconfigure SPprime @15:22:31.87 SP.seek 100ml,8:51 @15:24:26.25 Intake.close @15:24:27.00 Pressurizing cartridge to 26psi (try #1 of 3) @15:24:27.01 SP.setPosition! 0ml @15:24:27.06 PB.open @15:24:27.87 TV.seek :crack @15:24:28.66 TV.seek :clear @15:24:29.46 Intake.open @15:24:30.93 PB.close @15:24:31.66 Exhaust.close @15:24:32.45 Sea @-42.9psia @15:24:32.53 SP.reconfigure SPcharge @15:24:32.59 SP.seek 2.3ml,42 seconds @15:24:42.74 Cartridge pressurized with 0.481ml to 26.1psi @15:24:42.98 delay 3 seconds @15:24:46.04 Cartridge at 23.4psi 3 seconds later @15:24:46.12 TV.seek :cartridge @15:24:47.95 TV.seek :clear @15:24:49.76 Sampling 1000.0ml @15:24:49.77 SP.setPosition! 0ml @15:24:49.82 Exhaust.open @15:24:50.61 Sea @-42.9psia @15:24:50.69 SP.reconfigure SPsample @15:24:50.76 SP.seek 1000ml,1:23:53 @15:24:51.72 Cartridge 45 sampling 0/1000ml at 0.17ml/s with 7.4psi vacuum @15:25:51.73 Cartridge 45 sampling 20/1000ml at 0.29ml/s with 23.1psi vacuum @15:26:48.37 Flow averaged 0.30ml/s over 1:48 @15:26:48.39 Waiting up to 15 seconds for vacuum to reduce from 28.7 to 18.0psi @15:26:49.02 Relieved excess vacuum in 10.5 seconds @15:26:49.04 SP.seek 1000ml,1:21:10 @15:26:51.72 Cartridge 45 sampling 33/1000ml at 0.20ml/s with 25.2psi vacuum @15:26:54.56 Flow estimate reduced to 0.24ml/s @15:26:54.59 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @15:26:55.21 Relieved excess vacuum in 3.1 seconds @15:26:55.23 SP.seek 1000ml,1:21:05 @15:26:58.93 Flow estimate reduced to 0.19ml/s @15:26:58.95 Intake.close @15:26:58.99 Exhaust.close @15:26:59.80 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @15:26:59.90 Sampled 34.2ml @15:26:59.98 TV.seek :bypass @15:27:01.59 TV.seek :clear @15:27:03.64 -> Cmd.startProcessing @15:27:03.89 PV.seek :reagent @15:27:04.73 Sample loop closed. Ready to Deliver Reagents @15:27:04.74 ArS.seek :sealed @15:27:09.02 ArS.seek :evac @15:27:22.95 delay 20 seconds @15:27:42.98 ArS.seek :clear @15:28:00.69 RNAlater soak for 10:00 @15:38:00.71 ArS.seek :tVent @15:38:03.29 delay 1 second @15:38:04.31 ArS.seek :evac @15:38:19.94 delay 10 seconds @15:38:29.97 Sample preserved and evacuated @15:38:30.98 -> Cmd.stop @15:38:31.12 PV.seek :locked @15:38:32.26 PinPort.seek :clear @15:38:32.39 ArS.seek :clear @15:38:32.58 LyS2.seek :clear @15:38:32.72 AirS.seek :clear @15:38:50.16 ArS.coast @15:38:50.20 LyS1.coast @15:38:50.25 LyS2.coast @15:38:50.30 AirS.coast @15:38:50.35 TV.coast @15:38:50.40 SP.coast @15:38:50.45 SC.coast @15:38:50.50 PV.coast @15:38:50.56 PinPort.coast @15:38:50.74 Gate.power :motor,:OFF @15:38:50.75 Gate.power :main,:OFF @15:38:50.78 Gate.power :core,:OFF @15:38:50.80 Gate.power :analytic1,:OFF @15:38:50.82 Gate.power :analytic2,:OFF @15:38:50.84 Gate.power :analytic3,:OFF @15:38:50.86 Safely stopped and ready to power off ##-- file closed 2018-08-26T19:50:12.792Z ##-- file opened 2018-08-26T19:50:12.797Z @15:49:47.65 -> showStatus @15:49:49.65 -> Cmd.loadCartridge @15:49:49.78 Gate.power :main,:ON,limit to 5000mA @15:49:49.79 Gate.power :analytic1,:ON,limit to 5000mA @15:49:49.81 Gate.power :analytic2,:ON,limit to 5000mA @15:49:49.83 Gate.power :analytic3,:ON,limit to 5000mA @15:49:49.98 Powering core bus @15:49:52.28 SPR Module 1, FW Rev. B, FPGA Rev. C @15:49:52.38 ArS.configure ArSconfig @15:49:52.48 LyS1.configure LyS1config @15:49:52.60 LyS2.configure LyS2config @15:49:52.71 AirS.configure AirSconfig @15:49:52.82 TV.configure TVconfig @15:49:52.94 SP.configure SPconfig @15:49:53.06 SC.configure SCconfig @15:49:53.18 PV.configure PVconfig @15:49:53.30 PinPort.configure PPconfig @15:49:53.41 Sea.configure SPconfig @15:49:53.47 All elf controllers running firmware version 4.5 @15:49:53.49 Gate.power :motor,:ON,limit to 5000mA @15:49:53.61 ArS.reconfigure ArSconfig @15:49:53.94 ArS.home.jog 0 @15:49:54.08 PV.reconfigure PVconfig @15:49:54.14 TV.reconfigure TVconfig @15:49:54.17 AirS.reconfigure AirSconfig @15:49:54.21 LyS1.reconfigure LyS1config @15:49:54.24 PinPort.reconfigure PPconfig @15:49:54.30 LyS2.reconfigure LyS2config @15:49:54.46 TV.home.jog 0 @15:49:54.50 PV.home.jog 0 @15:49:54.57 PinPort.home.jog 0 @15:49:54.61 LyS2.home.jog 0 @15:49:54.65 LyS1.home.jog 0 @15:49:54.68 AirS.home.jog 0 @15:49:55.08 ArS.reconfigure HomeMagSyringe @15:49:55.41 PV.reconfigure HomePV @15:49:55.45 TV.reconfigure HomeTV @15:49:55.48 AirS.reconfigure HomeMagSyringe @15:49:55.52 ArS.jog -240 @15:49:55.55 LyS1.reconfigure HomeSyringe @15:49:55.59 PinPort.reconfigure HomePP @15:49:55.62 LyS2.reconfigure HomeSyringe @15:49:55.82 PV.jog -1481 @15:49:55.86 TV.jog -553 @15:49:55.89 AirS.jog -314 @15:49:55.93 LyS1.jog -259 @15:49:55.96 PinPort.jog -110 @15:49:56.01 LyS2.jog -266 @15:49:56.24 ArS.setRawPosition! -50 @15:49:56.29 ArS.seek :home @15:49:56.48 PinPort.setRawPosition! 0 @15:49:56.53 PinPort.seek :home @15:49:56.90 PinPort.reconfigure PPconfig @15:49:56.93 AirS.setRawPosition! -50 @15:49:56.96 LyS2.setRawPosition! -50 @15:49:56.98 LyS1.setRawPosition! -50 @15:49:57.01 ArS.reconfigure ArSconfig @15:49:57.14 AirS.seek :home @15:49:57.18 LyS2.seek :home @15:49:57.29 LyS1.seek :home @15:49:57.48 PV.setRawPosition! -50 @15:49:57.53 PV.seek :home @15:49:57.62 AirS.reconfigure AirSconfig @15:49:57.74 LyS2.reconfigure LyS2config @15:49:57.76 LyS1.reconfigure LyS1config @15:49:57.89 PV.reconfigure PVconfig @15:49:57.97 TV.setRawPosition! 0 @15:49:58.02 TV.seek :home @15:49:58.11 TV.reconfigure TVconfig @15:49:58.33 TV.seek :clear @15:49:59.95 TV.coast @15:50:00.00 SC.reconfigure HomeSC @15:50:00.06 SC.home.jog 0 @15:50:00.82 SC.reconfigure SCconfig @15:50:00.88 SC.seek 45 @15:50:00.97 Selecting Cartridge 44 @15:50:01.28 SC.seek 44 @15:50:01.78 LyS2.seek :locked @15:50:01.90 PV.seek :locked @15:50:02.03 AirS.seek :locked @15:50:06.12 Powering cartridge bus @15:50:08.24 Gate.setIR 1750,5000,0,6 @15:50:08.36 Gate.setIR 0,5000,0,6 @15:50:08.40 Loaded Archive Cartridge @15:50:08.41 Gate.power :cartridge,:OFF @15:50:27.98 -> Cmd.startFiltering @15:50:28.21 Duration of filtering limited to 1:05:00 @15:50:28.46 Priming sample loop w/100ml, bypass w/10ml @15:50:28.54 SP.setPosition! 0ml @15:50:28.58 Intake.open @15:50:28.62 Exhaust.open @15:50:29.42 Sea @-42.9psia @15:50:29.44 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @15:50:35.21 Vacuum tare is 24% of full scale @15:50:35.23 PB.open @15:50:35.26 Exhaust.close @15:50:36.07 Sea @-42.9psia @15:50:36.16 SP.reconfigure SPprime @15:50:36.22 SP.seek 10ml,1:21 @15:50:47.08 SP.setPosition! 0ml @15:50:47.13 PB.close @15:50:47.15 Exhaust.open @15:50:47.97 Sea @-42.9psia @15:50:48.05 SP.reconfigure SPprime @15:50:48.11 SP.seek 100ml,8:51 @15:52:42.78 Intake.close @15:52:43.49 Pressurizing cartridge to 26psi (try #1 of 3) @15:52:43.50 SP.setPosition! 0ml @15:52:43.55 PB.open @15:52:44.35 TV.seek :crack @15:52:45.14 TV.seek :clear @15:52:45.94 Intake.open @15:52:47.38 PB.close @15:52:48.11 Exhaust.close @15:52:48.85 Sea @-42.9psia @15:52:48.93 SP.reconfigure SPcharge @15:52:48.99 SP.seek 2.3ml,42 seconds @15:52:59.41 Cartridge pressurized with 0.507ml to 27.1psi @15:52:59.82 delay 3 seconds @15:53:02.89 Cartridge at 23.9psi 3 seconds later @15:53:02.97 TV.seek :cartridge @15:53:04.79 TV.seek :clear @15:53:06.61 Sampling 1000.0ml @15:53:06.62 SP.setPosition! 0ml @15:53:06.67 Exhaust.open @15:53:07.42 Sea @-42.9psia @15:53:07.51 SP.reconfigure SPsample @15:53:07.57 SP.seek 1000ml,1:23:53 @15:53:08.53 Cartridge 44 sampling 0/1000ml at 0.17ml/s with 6.9psi vacuum @15:54:08.54 Cartridge 44 sampling 22/1000ml at 0.31ml/s with 19.2psi vacuum @15:55:08.54 Cartridge 44 sampling 40/1000ml at 0.28ml/s with 19.7psi vacuum @15:56:08.54 Cartridge 44 sampling 54/1000ml at 0.20ml/s with 24.5psi vacuum @15:56:25.42 Flow averaged 0.30ml/s over 3:12 @15:56:25.44 Waiting up to 19 seconds for vacuum to reduce from 30.2 to 18.0psi @15:56:26.06 Relieved excess vacuum in 7.4 seconds @15:56:26.09 SP.seek 1000ml,1:19:03 @15:56:28.82 Flow estimate reduced to 0.24ml/s @15:56:28.84 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @15:56:29.46 Relieved excess vacuum in 3.3 seconds @15:56:29.49 SP.seek 1000ml,1:19:01 @15:56:32.63 Flow estimate reduced to 0.19ml/s @15:56:32.64 Intake.close @15:56:32.68 Exhaust.close @15:56:33.47 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @15:56:33.57 Sampled 58.9ml @15:56:33.65 TV.seek :bypass @15:56:35.26 TV.seek :clear @15:56:37.41 -> Cmd.startProcessing @15:56:37.67 PV.seek :reagent @15:56:38.52 Sample loop closed. Ready to Deliver Reagents @15:56:38.54 ArS.seek :sealed @15:56:43.04 ArS.seek :evac @15:56:56.98 delay 20 seconds @15:57:17.01 ArS.seek :clear @15:57:34.72 RNAlater soak for 10:00 @16:07:34.75 ArS.seek :tVent @16:07:37.32 delay 1 second @16:07:38.35 ArS.seek :evac @16:07:53.97 delay 10 seconds @16:08:04.01 Sample preserved and evacuated @16:08:05.19 -> Cmd.stop @16:08:05.34 PV.seek :locked @16:08:06.23 PinPort.seek :clear @16:08:06.37 ArS.seek :clear @16:08:06.55 LyS2.seek :clear @16:08:06.68 AirS.seek :clear @16:08:24.13 ArS.coast @16:08:24.18 LyS1.coast @16:08:24.23 LyS2.coast @16:08:24.27 AirS.coast @16:08:24.32 TV.coast @16:08:24.37 SP.coast @16:08:24.42 SC.coast @16:08:24.47 PV.coast @16:08:24.53 PinPort.coast @16:08:24.70 Gate.power :motor,:OFF @16:08:24.72 Gate.power :main,:OFF @16:08:24.74 Gate.power :core,:OFF @16:08:24.76 Gate.power :analytic1,:OFF @16:08:24.78 Gate.power :analytic2,:OFF @16:08:24.80 Gate.power :analytic3,:OFF @16:08:24.82 Safely stopped and ready to power off ##-- file closed 2018-08-26T20:19:51.072Z ##-- file opened 2018-08-26T20:19:51.074Z @16:19:23.22 -> showStatus @16:19:25.22 -> Cmd.loadCartridge @16:19:25.34 Gate.power :main,:ON,limit to 5000mA @16:19:25.35 Gate.power :analytic1,:ON,limit to 5000mA @16:19:25.38 Gate.power :analytic2,:ON,limit to 5000mA @16:19:25.40 Gate.power :analytic3,:ON,limit to 5000mA @16:19:25.54 Powering core bus @16:19:27.84 SPR Module 1, FW Rev. B, FPGA Rev. C @16:19:27.93 ArS.configure ArSconfig @16:19:28.03 LyS1.configure LyS1config @16:19:28.15 LyS2.configure LyS2config @16:19:28.25 AirS.configure AirSconfig @16:19:28.37 TV.configure TVconfig @16:19:28.49 SP.configure SPconfig @16:19:28.61 SC.configure SCconfig @16:19:28.73 PV.configure PVconfig @16:19:28.85 PinPort.configure PPconfig @16:19:28.97 Sea.configure SPconfig @16:19:29.03 All elf controllers running firmware version 4.5 @16:19:29.04 Gate.power :motor,:ON,limit to 5000mA @16:19:29.16 ArS.reconfigure ArSconfig @16:19:29.43 ArS.home.jog 0 @16:19:29.60 PV.reconfigure PVconfig @16:19:29.67 TV.reconfigure TVconfig @16:19:29.71 LyS2.reconfigure LyS2config @16:19:29.80 AirS.reconfigure AirSconfig @16:19:29.83 LyS1.reconfigure LyS1config @16:19:29.86 PinPort.reconfigure PPconfig @16:19:30.03 TV.home.jog 0 @16:19:30.09 LyS2.home.jog 0 @16:19:30.11 PV.home.jog 0 @16:19:30.14 LyS1.home.jog 0 @16:19:30.17 AirS.home.jog 0 @16:19:30.19 PinPort.home.jog 0 @16:19:30.57 ArS.reconfigure HomeMagSyringe @16:19:30.93 TV.reconfigure HomeTV @16:19:30.95 LyS2.reconfigure HomeSyringe @16:19:30.98 ArS.jog -261 @16:19:31.03 PinPort.reconfigure HomePP @16:19:31.05 PV.reconfigure HomePV @16:19:31.08 AirS.reconfigure HomeMagSyringe @16:19:31.13 LyS1.reconfigure HomeSyringe @16:19:31.34 TV.jog -554 @16:19:31.38 LyS2.jog -265 @16:19:31.41 PV.jog -1477 @16:19:31.44 PinPort.jog -110 @16:19:31.47 AirS.jog -318 @16:19:31.49 LyS1.jog -266 @16:19:31.75 ArS.setRawPosition! -50 @16:19:31.79 ArS.seek :home @16:19:31.95 PinPort.setRawPosition! 0 @16:19:32.00 PinPort.seek :home @16:19:32.33 PinPort.reconfigure PPconfig @16:19:32.39 LyS2.setRawPosition! -50 @16:19:32.41 ArS.reconfigure ArSconfig @16:19:32.50 LyS2.seek :home @16:19:32.65 AirS.setRawPosition! -50 @16:19:32.66 LyS1.setRawPosition! -50 @16:19:32.75 AirS.seek :home @16:19:32.79 LyS1.seek :home @16:19:32.96 PV.setRawPosition! -50 @16:19:32.98 LyS2.reconfigure LyS2config @16:19:33.05 PV.seek :home @16:19:33.20 AirS.reconfigure AirSconfig @16:19:33.22 LyS1.reconfigure LyS1config @16:19:33.38 PV.reconfigure PVconfig @16:19:33.47 TV.setRawPosition! 0 @16:19:33.52 TV.seek :home @16:19:33.61 TV.reconfigure TVconfig @16:19:33.79 TV.seek :clear @16:19:35.40 TV.coast @16:19:35.46 SC.reconfigure HomeSC @16:19:35.52 SC.home.jog 0 @16:19:36.29 SC.reconfigure SCconfig @16:19:36.35 SC.seek 44 @16:19:36.43 Selecting Cartridge 43 @16:19:36.74 SC.seek 43 @16:19:37.24 LyS2.seek :locked @16:19:37.37 PV.seek :locked @16:19:37.50 AirS.seek :locked @16:19:41.58 Powering cartridge bus @16:19:43.71 Gate.setIR 1750,5000,0,6 @16:19:43.83 Gate.setIR 0,5000,0,6 @16:19:43.88 Loaded Lyse-n-Go Cartridge @16:19:43.89 PuckHeater.configure HeatConfig @16:19:43.95 Gate.power :heat,:ON,limit to 5000mA @16:20:03.94 -> Cmd.startFiltering @16:20:04.16 Duration of filtering limited to 1:05:00 @16:20:04.41 Priming sample loop w/100ml, bypass w/10ml @16:20:04.49 SP.setPosition! 0ml @16:20:04.53 Intake.open @16:20:04.57 Exhaust.open @16:20:05.37 Sea @-42.9psia @16:20:05.39 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @16:20:10.92 Vacuum tare is 24% of full scale @16:20:11.18 PB.open @16:20:11.22 Exhaust.close @16:20:12.04 Sea @-42.9psia @16:20:12.12 SP.reconfigure SPprime @16:20:12.19 SP.seek 10ml,1:21 @16:20:23.05 SP.setPosition! 0ml @16:20:23.09 PB.close @16:20:23.12 Exhaust.open @16:20:23.94 Sea @-42.9psia @16:20:24.02 SP.reconfigure SPprime @16:20:24.08 SP.seek 100ml,8:51 @16:22:18.50 Intake.close @16:22:19.26 Pressurizing cartridge to 26psi (try #1 of 3) @16:22:19.27 SP.setPosition! 0ml @16:22:19.32 PB.open @16:22:20.09 TV.seek :crack @16:22:20.88 TV.seek :clear @16:22:21.68 Intake.open @16:22:23.15 PB.close @16:22:23.83 Exhaust.close @16:22:24.56 Sea @-42.9psia @16:22:24.64 SP.reconfigure SPcharge @16:22:24.71 SP.seek 2.3ml,42 seconds @16:22:33.79 Cartridge pressurized with 0.385ml to 27.0psi @16:22:34.17 delay 3 seconds @16:22:37.23 Cartridge at 23.2psi 3 seconds later @16:22:37.32 TV.seek :cartridge @16:22:39.14 TV.seek :clear @16:22:40.96 Sampling 1000.0ml @16:22:40.97 SP.setPosition! 0ml @16:22:41.03 Exhaust.open @16:22:41.77 Sea @-42.9psia @16:22:41.86 SP.reconfigure SPsample @16:22:41.93 SP.seek 1000ml,1:23:53 @16:22:42.88 Cartridge 43 sampling 0/1000ml at 0.17ml/s with 5.6psi vacuum @16:23:42.89 Cartridge 43 sampling 32/1000ml at 0.55ml/s with 25.2psi vacuum @16:24:42.89 Cartridge 43 sampling 64/1000ml at 0.53ml/s with 26.8psi vacuum @16:25:42.89 Cartridge 43 sampling 96/1000ml at 0.51ml/s with 27.4psi vacuum @16:26:42.89 Cartridge 43 sampling 128/1000ml at 0.54ml/s with 24.2psi vacuum @16:27:42.89 Cartridge 43 sampling 159/1000ml at 0.50ml/s with 24.4psi vacuum @16:28:42.89 Cartridge 43 sampling 190/1000ml at 0.50ml/s with 26.9psi vacuum @16:29:42.89 Cartridge 43 sampling 220/1000ml at 0.52ml/s with 23.9psi vacuum @16:30:42.89 Cartridge 43 sampling 249/1000ml at 0.47ml/s with 17.3psi vacuum @16:31:42.89 Cartridge 43 sampling 278/1000ml at 0.49ml/s with 24.5psi vacuum @16:32:42.89 Cartridge 43 sampling 306/1000ml at 0.46ml/s with 23.2psi vacuum @16:33:42.89 Cartridge 43 sampling 334/1000ml at 0.47ml/s with 20.6psi vacuum @16:34:42.89 Cartridge 43 sampling 360/1000ml at 0.45ml/s with 22.1psi vacuum @16:35:42.89 Cartridge 43 sampling 386/1000ml at 0.43ml/s with 20.9psi vacuum @16:36:42.89 Cartridge 43 sampling 410/1000ml at 0.38ml/s with 17.8psi vacuum @16:37:42.89 Cartridge 43 sampling 434/1000ml at 0.38ml/s with 19.2psi vacuum @16:38:43.14 Cartridge 43 sampling 456/1000ml at 0.34ml/s with 17.7psi vacuum @16:39:42.89 Cartridge 43 sampling 476/1000ml at 0.36ml/s with 22.8psi vacuum @16:40:42.89 Cartridge 43 sampling 494/1000ml at 0.27ml/s with 21.3psi vacuum @16:41:42.89 Cartridge 43 sampling 512/1000ml at 0.30ml/s with 24.8psi vacuum @16:42:42.89 Cartridge 43 sampling 528/1000ml at 0.29ml/s with 20.2psi vacuum @16:43:42.89 Cartridge 43 sampling 543/1000ml at 0.24ml/s with 23.7psi vacuum @16:44:42.89 Cartridge 43 sampling 557/1000ml at 0.26ml/s with 20.7psi vacuum @16:45:42.89 Cartridge 43 sampling 571/1000ml at 0.20ml/s with 26.3psi vacuum @16:46:42.89 Cartridge 43 sampling 583/1000ml at 0.20ml/s with 24.8psi vacuum @16:47:42.89 Cartridge 43 sampling 596/1000ml at 0.20ml/s with 27.6psi vacuum @16:48:42.89 Cartridge 43 sampling 608/1000ml at 0.20ml/s with 24.6psi vacuum @16:49:42.89 Cartridge 43 sampling 620/1000ml at 0.20ml/s with 29.1psi vacuum @16:50:41.50 Flow averaged 0.38ml/s over 27:52 @16:50:41.53 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @16:50:42.15 Relieved excess vacuum in 9.1 seconds @16:50:42.17 SP.seek 1000ml,31:10 @16:50:42.88 Cartridge 43 sampling 632/1000ml at 0.12ml/s with 10.8psi vacuum @16:51:19.15 Flow averaged 0.22ml/s over 34 seconds @16:51:19.17 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @16:51:19.78 Relieved excess vacuum in 4.5 seconds @16:51:19.81 SP.seek 1000ml,30:33 @16:51:36.17 Flow averaged 0.24ml/s over 14 seconds @16:51:36.19 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @16:51:36.81 Relieved excess vacuum in 3.9 seconds @16:51:36.84 SP.seek 1000ml,30:17 @16:51:42.89 Cartridge 43 sampling 644/1000ml at 0.20ml/s with 26.3psi vacuum @16:51:53.17 Flow averaged 0.20ml/s over 17 seconds @16:51:53.18 Intake.close @16:51:53.24 Exhaust.close @16:51:54.00 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @16:51:54.10 Sampled 646.1ml @16:51:54.18 TV.seek :bypass @16:51:55.79 TV.seek :clear @16:51:57.91 -> Cmd.startProcessing @16:51:59.70 SPR.command \"read_sequences\" @16:51:59.95 SPR.command \"stats_on\" @16:51:59.98 SPR.command \"start 0 1\" @16:52:01.99 Begun log /var/log/esp/spr18aug2616h.spr @16:52:02.80 Handoff.select :waste @16:52:02.92 ArS.seek empty @16:52:20.62 PinPort.seek :cartridge @16:52:26.33 LyS1.seek 1350ul @16:52:32.27 PV.seek :reagent @16:52:33.11 AirS.seek :tVent @16:52:35.17 delay 2 seconds @16:52:37.19 AirS.reconfigure EvacAirS @16:52:37.25 AirS.seek :evac @16:52:59.80 delay 15 seconds @16:53:14.82 AirS.reconfigure AirSconfig @16:53:14.88 AirS.seek :tVent @16:53:29.09 delay 2 seconds @16:53:31.11 AirS.reconfigure EvacAirS @16:53:31.18 AirS.seek :evac @16:53:53.47 delay 15 seconds @16:54:08.49 AirS.reconfigure AirSconfig @16:54:08.55 AirS.seek :tVent @16:54:22.76 delay 2 seconds @16:54:24.78 AirS.reconfigure EvacAirS @16:54:24.84 AirS.seek :evac @16:54:47.12 delay 15 seconds @16:55:02.14 AirS.reconfigure AirSconfig @16:55:02.20 AirS.seek :tVent @16:55:16.41 delay 2 seconds @16:55:18.43 AirS.reconfigure EvacAirS @16:55:18.49 AirS.seek :evac @16:55:40.79 delay 15 seconds @16:55:55.82 AirS.reconfigure AirSconfig @16:55:55.88 AirS.seek :tVent @16:56:10.10 delay 2 seconds @16:56:12.12 AirS.reconfigure EvacAirS @16:56:12.18 AirS.seek :evac @16:56:34.47 delay 15 seconds @16:56:49.49 delay 1:30 @16:58:19.51 AirS.reconfigure AirSconfig @16:58:19.57 AirS.seek :fVent @16:58:21.01 delay 15 seconds @16:58:36.03 LyS1.reconfigure SlowLyS1 @16:58:36.13 LyS1.seek 1050ul @16:59:06.19 delay 5 seconds @16:59:11.21 Handoff.select :blocked @16:59:11.41 LyS1.seek 450ul @17:00:11.35 delay 10 seconds @17:00:21.38 AirS.reconfigure SlowAirS @17:00:21.49 AirS.reconfigure to mix into 1.37806 parts LyS1 @17:00:21.85 AirS.seek evac @17:00:21.88 LyS1.seek 300ul @17:00:36.99 AirS.reconfigure AirSconfig @17:00:37.04 AirS.seek :full @17:00:51.26 LyS1.seek :empty @17:01:21.36 PuckHeater.seek 84.2C @17:02:22.57 PuckHeater.hold 15:00,84.2C @17:17:21.78 LyS1.reconfigure LyS1config @17:17:21.84 LyS1.seek 900ul @17:17:30.18 AirS.seek :tVent @17:17:30.26 delay 2 seconds @17:17:32.28 AirS.reconfigure HalfSpeedAirS @17:17:32.34 AirS.seek :evac @17:18:00.07 delay 15 seconds @17:18:15.29 AirS.reconfigure AirSconfig @17:18:15.40 AirS.seek :tVent @17:18:29.61 delay 2 seconds @17:18:31.63 AirS.reconfigure HalfSpeedAirS @17:18:31.69 AirS.seek :evac @17:18:59.42 delay 15 seconds @17:19:14.44 AirS.reconfigure AirSconfig @17:19:14.50 AirS.seek :tVent @17:19:28.71 delay 2 seconds @17:19:30.73 AirS.reconfigure HalfSpeedAirS @17:19:30.79 AirS.seek :evac @17:19:58.53 delay 15 seconds @17:20:13.55 delay 1:00 @17:21:13.57 Handoff.select :waste @17:21:13.67 delay 15 seconds @17:21:28.69 AirS.seek :fVent @17:21:30.89 delay 10 seconds @17:21:40.91 AirS.reconfigure AirSconfig @17:21:40.96 AirS.seek :evac @17:21:42.40 Lysis Recovered in LyS1 @17:21:42.42 Lysis Complete @17:21:42.43 Handoff.select :waste @17:21:42.46 LyS2.seek :full @17:21:42.54 LyS1.reconfigure DAmixLyS1 @17:21:42.60 LyS2.reconfigure DAmixLyS2 @17:21:42.98 LyS2.seek 1480ul @17:21:43.02 LyS1.seek 650ul @17:22:30.33 delay 5 seconds @17:22:35.35 Handoff.select :SPR @17:22:35.47 SPR.command \"start 2 14\" @17:22:36.76 Running Experiment 2 @17:22:36.77 delay 10 seconds @17:22:46.79 SPR.command \"start 2 0\" @17:22:50.12 Waiting up to 11:00 for SPR to enter state 1 @17:30:08.41 SPR.command \"hold_on\" @17:30:10.60 LyS2.seek 1130ul @17:30:10.63 LyS1.seek 300ul @17:31:16.80 delay 15 seconds @17:31:31.82 SPR.command \"hold_off\" @17:31:35.06 Waiting up to 25:00 for SPR to enter state 7 @17:43:10.61 Handoff.select :waste @17:43:10.73 LyS1.reconfigure LyS1config @17:43:10.79 LyS2.reconfigure LyS2config @17:43:10.85 Rep #1 SPR Analysis has Completed @17:43:11.55 Ended log /var/log/esp/spr18aug2616h.spr @17:43:11.56 SPR.command \"stats_off\" @17:43:13.66 -> Cmd.stop @17:43:13.74 Handoff.select :close @17:43:13.92 Gate.power :heat,:OFF @17:43:13.93 Gate.power :cartridge,:OFF @17:43:13.96 PV.seek :locked @17:43:14.85 PinPort.seek :clear @17:43:20.62 ArS.seek :clear @17:43:21.00 LyS1.seek :clear @17:43:21.13 LyS2.seek :clear @17:43:21.26 AirS.seek :clear @17:43:38.60 ArS.coast @17:43:38.65 LyS1.coast @17:43:38.70 LyS2.coast @17:43:38.75 AirS.coast @17:43:38.80 TV.coast @17:43:38.85 SP.coast @17:43:38.90 SC.coast @17:43:38.95 PV.coast @17:43:39.00 PinPort.coast @17:43:39.19 Gate.power :motor,:OFF @17:43:39.20 Gate.power :main,:OFF @17:43:39.23 Gate.power :core,:OFF @17:43:39.25 Gate.power :analytic1,:OFF @17:43:39.27 Gate.power :analytic2,:OFF @17:43:39.29 Gate.power :analytic3,:OFF @17:43:39.31 Safely stopped and ready to power off ##-- file closed 2018-08-26T21:55:03.680Z ##-- file opened 2018-08-26T21:55:03.681Z @17:54:27.08 -> showStatus @17:54:29.09 -> Cmd.loadCartridge @17:54:29.21 Gate.power :main,:ON,limit to 5000mA @17:54:29.23 Gate.power :analytic1,:ON,limit to 5000mA @17:54:29.25 Gate.power :analytic2,:ON,limit to 5000mA @17:54:29.27 Gate.power :analytic3,:ON,limit to 5000mA @17:54:29.41 Powering core bus @17:54:31.71 SPR Module 1, FW Rev. B, FPGA Rev. C @17:54:31.80 ArS.configure ArSconfig @17:54:31.91 LyS1.configure LyS1config @17:54:32.03 LyS2.configure LyS2config @17:54:32.14 AirS.configure AirSconfig @17:54:32.26 TV.configure TVconfig @17:54:32.38 SP.configure SPconfig @17:54:32.50 SC.configure SCconfig @17:54:32.62 PV.configure PVconfig @17:54:32.74 PinPort.configure PPconfig @17:54:32.86 Sea.configure SPconfig @17:54:32.92 All elf controllers running firmware version 4.5 @17:54:32.94 Gate.power :motor,:ON,limit to 5000mA @17:54:33.10 ArS.reconfigure ArSconfig @17:54:33.34 ArS.home.jog 0 @17:54:33.49 PV.reconfigure PVconfig @17:54:33.57 TV.reconfigure TVconfig @17:54:33.60 LyS2.reconfigure LyS2config @17:54:33.64 PinPort.reconfigure PPconfig @17:54:33.67 LyS1.reconfigure LyS1config @17:54:33.72 AirS.reconfigure AirSconfig @17:54:33.93 PV.home.jog 0 @17:54:33.96 TV.home.jog 0 @17:54:33.99 LyS2.home.jog 0 @17:54:34.01 LyS1.home.jog 0 @17:54:34.04 PinPort.home.jog 0 @17:54:34.07 AirS.home.jog 0 @17:54:34.62 ArS.reconfigure HomeMagSyringe @17:54:34.79 PV.reconfigure HomePV @17:54:34.83 TV.reconfigure HomeTV @17:54:34.91 LyS1.reconfigure HomeSyringe @17:54:34.96 PinPort.reconfigure HomePP @17:54:35.00 LyS2.reconfigure HomeSyringe @17:54:35.03 ArS.jog -245 @17:54:35.08 AirS.reconfigure HomeMagSyringe @17:54:35.27 PV.jog -1479 @17:54:35.33 TV.jog -557 @17:54:35.36 LyS1.jog -259 @17:54:35.39 PinPort.jog -109 @17:54:35.42 LyS2.jog -265 @17:54:35.46 AirS.jog -327 @17:54:35.76 ArS.setRawPosition! -50 @17:54:35.80 ArS.seek :home @17:54:35.90 PinPort.setRawPosition! 0 @17:54:35.94 PinPort.seek :home @17:54:36.03 PinPort.reconfigure PPconfig @17:54:36.38 AirS.setRawPosition! -50 @17:54:36.41 LyS1.setRawPosition! -50 @17:54:36.43 ArS.reconfigure ArSconfig @17:54:36.46 LyS2.setRawPosition! -50 @17:54:36.57 AirS.seek :home @17:54:36.67 LyS1.seek :home @17:54:36.70 LyS2.seek :home @17:54:36.89 PV.setRawPosition! -50 @17:54:36.93 PV.seek :home @17:54:37.05 AirS.reconfigure AirSconfig @17:54:37.15 LyS2.reconfigure LyS2config @17:54:37.18 LyS1.reconfigure LyS1config @17:54:37.31 PV.reconfigure PVconfig @17:54:37.39 TV.setRawPosition! 0 @17:54:37.44 TV.seek :home @17:54:37.54 TV.reconfigure TVconfig @17:54:37.75 TV.seek :clear @17:54:39.37 TV.coast @17:54:39.42 SC.reconfigure HomeSC @17:54:39.48 SC.home.jog 0 @17:54:40.25 SC.reconfigure SCconfig @17:54:40.30 SC.seek 43 @17:54:40.40 Selecting Cartridge 42 @17:54:40.71 SC.seek 42 @17:54:41.21 LyS2.seek :locked @17:54:41.34 PV.seek :locked @17:54:41.47 AirS.seek :locked @17:54:45.55 Powering cartridge bus @17:54:47.68 Gate.setIR 1750,5000,0,6 @17:54:47.80 Gate.setIR 0,5000,0,6 @17:54:47.83 Loaded Archive Cartridge @17:54:47.84 Gate.power :cartridge,:OFF @17:55:07.40 -> Cmd.startFiltering @17:55:07.62 Duration of filtering limited to 1:05:00 @17:55:07.87 Priming sample loop w/100ml, bypass w/10ml @17:55:07.95 SP.setPosition! 0ml @17:55:07.99 Intake.open @17:55:08.02 Exhaust.open @17:55:08.85 Sea @-42.9psia @17:55:08.86 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @17:55:14.64 Vacuum tare is 25% of full scale @17:55:14.66 PB.open @17:55:14.70 Exhaust.close @17:55:15.47 Sea @-42.9psia @17:55:15.55 SP.reconfigure SPprime @17:55:15.61 SP.seek 10ml,1:21 @17:55:26.48 SP.setPosition! 0ml @17:55:26.52 PB.close @17:55:26.55 Exhaust.open @17:55:27.36 Sea @-42.9psia @17:55:27.44 SP.reconfigure SPprime @17:55:27.51 SP.seek 100ml,8:51 @17:57:21.75 Intake.close @17:57:22.49 Pressurizing cartridge to 26psi (try #1 of 3) @17:57:22.50 SP.setPosition! 0ml @17:57:22.55 PB.open @17:57:23.34 TV.seek :crack @17:57:24.13 TV.seek :clear @17:57:24.93 Intake.open @17:57:26.38 PB.close @17:57:27.08 Exhaust.close @17:57:27.85 Sea @-42.9psia @17:57:27.93 SP.reconfigure SPcharge @17:57:27.99 SP.seek 2.3ml,42 seconds @17:57:34.59 Cartridge pressurized with 0.201ml to 26.2psi @17:57:34.81 delay 3 seconds @17:57:37.89 Cartridge at 21.5psi 3 seconds later @17:57:37.97 TV.seek :cartridge @17:57:39.79 TV.seek :clear @17:57:41.61 Sampling 1000.0ml @17:57:41.62 SP.setPosition! 0ml @17:57:41.67 Exhaust.open @17:57:42.43 Sea @-42.9psia @17:57:42.51 SP.reconfigure SPsample @17:57:42.58 SP.seek 1000ml,1:23:53 @17:57:43.54 Cartridge 42 sampling 0/1000ml at 0.17ml/s with 5.9psi vacuum @17:58:43.55 Cartridge 42 sampling 23/1000ml at 0.38ml/s with 19.2psi vacuum @17:59:43.55 Cartridge 42 sampling 44/1000ml at 0.35ml/s with 22.9psi vacuum @18:00:43.55 Cartridge 42 sampling 62/1000ml at 0.31ml/s with 25.4psi vacuum @18:01:43.55 Cartridge 42 sampling 77/1000ml at 0.25ml/s with 18.6psi vacuum @18:02:43.55 Cartridge 42 sampling 90/1000ml at 0.24ml/s with 23.6psi vacuum @18:03:39.63 Flow averaged 0.29ml/s over 5:52 @18:03:39.65 Waiting up to 15 seconds for vacuum to reduce from 28.9 to 18.0psi @18:03:40.26 Relieved excess vacuum in 6.5 seconds @18:03:40.29 SP.seek 1000ml,1:15:24 @18:03:43.54 Cartridge 42 sampling 102/1000ml at 0.20ml/s with 29.7psi vacuum @18:03:53.61 Flow estimate reduced to 0.23ml/s @18:03:53.64 Waiting up to 17 seconds for vacuum to reduce from 29.8 to 18.0psi @18:03:54.25 Relieved excess vacuum in 10.6 seconds @18:03:54.28 SP.seek 1000ml,1:15:11 @18:03:58.05 Flow estimate reduced to 0.19ml/s @18:03:58.07 Intake.close @18:03:58.11 Exhaust.close @18:03:58.89 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @18:03:58.98 Sampled 104.9ml @18:03:59.06 TV.seek :bypass @18:04:00.68 TV.seek :clear @18:04:02.99 -> Cmd.startProcessing @18:04:03.49 PV.seek :reagent @18:04:04.35 Sample loop closed. Ready to Deliver Reagents @18:04:04.37 ArS.seek :sealed @18:04:08.64 ArS.seek :evac @18:04:22.58 delay 20 seconds @18:04:42.61 ArS.seek :clear @18:05:00.34 RNAlater soak for 10:00 @18:15:00.37 ArS.seek :tVent @18:15:02.95 delay 1 second @18:15:03.97 ArS.seek :evac @18:15:19.59 delay 10 seconds @18:15:29.62 Sample preserved and evacuated @18:15:30.83 -> Cmd.stop @18:15:30.97 PV.seek :locked @18:15:31.86 PinPort.seek :clear @18:15:32.00 ArS.seek :clear @18:15:32.18 LyS2.seek :clear @18:15:32.31 AirS.seek :clear @18:15:49.75 ArS.coast @18:15:49.80 LyS1.coast @18:15:49.85 LyS2.coast @18:15:49.90 AirS.coast @18:15:49.95 TV.coast @18:15:50.00 SP.coast @18:15:50.05 SC.coast @18:15:50.10 PV.coast @18:15:50.14 PinPort.coast @18:15:50.33 Gate.power :motor,:OFF @18:15:50.34 Gate.power :main,:OFF @18:15:50.37 Gate.power :core,:OFF @18:15:50.39 Gate.power :analytic1,:OFF @18:15:50.41 Gate.power :analytic2,:OFF @18:15:50.43 Gate.power :analytic3,:OFF @18:15:50.45 Safely stopped and ready to power off ##-- file closed 2018-08-27T01:32:16.725Z ##-- file opened 2018-08-27T01:32:16.732Z @21:31:20.10 -> showStatus @21:31:22.11 -> Cmd.loadCartridge @21:31:22.23 Gate.power :main,:ON,limit to 5000mA @21:31:22.25 Gate.power :analytic1,:ON,limit to 5000mA @21:31:22.27 Gate.power :analytic2,:ON,limit to 5000mA @21:31:22.29 Gate.power :analytic3,:ON,limit to 5000mA @21:31:22.43 Powering core bus @21:31:24.73 SPR Module 1, FW Rev. B, FPGA Rev. C @21:31:24.83 ArS.configure ArSconfig @21:31:24.93 LyS1.configure LyS1config @21:31:25.04 LyS2.configure LyS2config @21:31:25.15 AirS.configure AirSconfig @21:31:25.27 TV.configure TVconfig @21:31:25.39 SP.configure SPconfig @21:31:25.51 SC.configure SCconfig @21:31:25.62 PV.configure PVconfig @21:31:25.74 PinPort.configure PPconfig @21:31:25.86 Sea.configure SPconfig @21:31:25.92 All elf controllers running firmware version 4.5 @21:31:25.93 Gate.power :motor,:ON,limit to 5000mA @21:31:26.10 ArS.reconfigure ArSconfig @21:31:26.39 ArS.home.jog 0 @21:31:26.52 PinPort.reconfigure PPconfig @21:31:26.59 PV.reconfigure PVconfig @21:31:26.62 AirS.reconfigure AirSconfig @21:31:26.65 LyS1.reconfigure LyS1config @21:31:26.70 TV.reconfigure TVconfig @21:31:26.76 LyS2.reconfigure LyS2config @21:31:26.97 PinPort.home.jog 0 @21:31:27.04 AirS.home.jog 0 @21:31:27.07 PV.home.jog 0 @21:31:27.11 TV.home.jog 0 @21:31:27.15 LyS1.home.jog 0 @21:31:27.19 LyS2.home.jog 0 @21:31:27.55 ArS.reconfigure HomeMagSyringe @21:31:27.73 ArS.jog -249 @21:31:27.89 PV.reconfigure HomePV @21:31:27.92 TV.reconfigure HomeTV @21:31:27.96 AirS.reconfigure HomeMagSyringe @21:31:27.99 LyS2.reconfigure HomeSyringe @21:31:28.03 PinPort.reconfigure HomePP @21:31:28.11 LyS1.reconfigure HomeSyringe @21:31:28.25 PV.jog -1475 @21:31:28.29 AirS.jog -312 @21:31:28.32 TV.jog -544 @21:31:28.35 PinPort.jog -110 @21:31:28.39 LyS2.jog -263 @21:31:28.46 LyS1.jog -262 @21:31:28.64 ArS.setRawPosition! -50 @21:31:28.68 ArS.seek :home @21:31:28.86 PinPort.setRawPosition! 0 @21:31:28.91 PinPort.seek :home @21:31:29.01 PinPort.reconfigure PPconfig @21:31:29.28 ArS.reconfigure ArSconfig @21:31:29.41 LyS2.setRawPosition! -50 @21:31:29.45 LyS1.setRawPosition! -50 @21:31:29.49 AirS.setRawPosition! -50 @21:31:29.54 LyS2.seek :home @21:31:29.62 LyS1.seek :home @21:31:29.73 AirS.seek :home @21:31:29.83 PV.setRawPosition! -50 @21:31:29.88 PV.seek :home @21:31:30.00 LyS1.reconfigure LyS1config @21:31:30.03 LyS2.reconfigure LyS2config @21:31:30.18 AirS.reconfigure AirSconfig @21:31:30.20 PV.reconfigure PVconfig @21:31:30.36 TV.setRawPosition! 0 @21:31:30.41 TV.seek :home @21:31:30.51 TV.reconfigure TVconfig @21:31:30.77 TV.seek :clear @21:31:32.39 TV.coast @21:31:32.44 SC.reconfigure HomeSC @21:31:32.51 SC.home.jog 0 @21:31:33.27 SC.reconfigure SCconfig @21:31:33.33 SC.seek 42 @21:31:33.43 Selecting Cartridge 41 @21:31:33.74 SC.seek 41 @21:31:34.23 LyS2.seek :locked @21:31:34.36 PV.seek :locked @21:31:34.49 AirS.seek :locked @21:31:38.57 Powering cartridge bus @21:31:40.71 Gate.setIR 1750,5000,0,6 @21:31:40.83 Gate.setIR 0,5000,0,6 @21:31:40.87 Loaded Archive Cartridge @21:31:40.88 Gate.power :cartridge,:OFF @21:32:00.43 -> Cmd.startFiltering @21:32:00.66 Duration of filtering limited to 1:05:00 @21:32:00.91 Priming sample loop w/100ml, bypass w/10ml @21:32:00.98 SP.setPosition! 0ml @21:32:01.02 Intake.open @21:32:01.07 Exhaust.open @21:32:01.88 Sea @-42.9psia @21:32:01.90 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @21:32:07.42 Vacuum tare is 23% of full scale @21:32:07.44 PB.open @21:32:07.47 Exhaust.close @21:32:08.50 Sea @-42.9psia @21:32:08.58 SP.reconfigure SPprime @21:32:08.65 SP.seek 10ml,1:21 @21:32:19.51 SP.setPosition! 0ml @21:32:19.56 PB.close @21:32:19.58 Exhaust.open @21:32:20.41 Sea @-42.9psia @21:32:20.49 SP.reconfigure SPprime @21:32:20.56 SP.seek 100ml,8:51 @21:34:15.15 Intake.close @21:34:15.90 Pressurizing cartridge to 26psi (try #1 of 3) @21:34:15.91 SP.setPosition! 0ml @21:34:15.96 PB.open @21:34:16.75 TV.seek :crack @21:34:17.54 TV.seek :clear @21:34:18.34 Intake.open @21:34:19.78 PB.close @21:34:20.51 Exhaust.close @21:34:21.29 Sea @-42.9psia @21:34:21.38 SP.reconfigure SPcharge @21:34:21.44 SP.seek 2.3ml,42 seconds @21:34:28.66 Cartridge pressurized with 0.241ml to 26.1psi @21:34:28.89 delay 3 seconds @21:34:31.96 Cartridge at 22.1psi 3 seconds later @21:34:32.04 TV.seek :cartridge @21:34:33.86 TV.seek :clear @21:34:35.68 Sampling 1000.0ml @21:34:35.69 SP.setPosition! 0ml @21:34:35.74 Exhaust.open @21:34:36.49 Sea @-42.9psia @21:34:36.58 SP.reconfigure SPsample @21:34:36.65 SP.seek 1000ml,1:23:53 @21:34:37.60 Cartridge 41 sampling 0/1000ml at 0.17ml/s with 7.3psi vacuum @21:35:37.61 Cartridge 41 sampling 21/1000ml at 0.31ml/s with 26.0psi vacuum @21:36:37.60 Cartridge 41 sampling 37/1000ml at 0.22ml/s with 22.0psi vacuum @21:37:34.67 Flow averaged 0.29ml/s over 2:52 @21:37:34.69 Waiting up to 22 seconds for vacuum to reduce from 31.4 to 18.0psi @21:37:35.31 Relieved excess vacuum in 7.5 seconds @21:37:35.33 SP.seek 1000ml,1:19:48 @21:37:37.60 Cartridge 41 sampling 49/1000ml at 0.20ml/s with 29.2psi vacuum @21:37:42.26 Flow estimate reduced to 0.23ml/s @21:37:42.28 Waiting up to 27 seconds for vacuum to reduce from 33.4 to 18.0psi @21:37:42.90 Relieved excess vacuum in 4.5 seconds @21:37:42.93 SP.seek 1000ml,1:19:42 @21:37:48.31 Flow estimate reduced to 0.18ml/s @21:37:48.32 Intake.close @21:37:48.37 Exhaust.close @21:37:49.17 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @21:37:49.26 Sampled 51.2ml @21:37:49.34 TV.seek :bypass @21:37:50.96 TV.seek :clear @21:37:53.11 -> Cmd.startProcessing @21:37:53.36 PV.seek :reagent @21:37:54.21 Sample loop closed. Ready to Deliver Reagents @21:37:54.23 ArS.seek :sealed @21:37:58.51 ArS.seek :evac @21:38:12.44 delay 20 seconds @21:38:32.72 ArS.seek :clear @21:38:50.44 RNAlater soak for 10:00 @21:48:50.47 ArS.seek :tVent @21:48:53.04 delay 1 second @21:48:54.07 ArS.seek :evac @21:49:09.69 delay 10 seconds @21:49:19.72 Sample preserved and evacuated @21:49:20.91 -> Cmd.stop @21:49:21.06 PV.seek :locked @21:49:21.95 PinPort.seek :clear @21:49:22.09 ArS.seek :clear @21:49:22.29 LyS2.seek :clear @21:49:22.41 AirS.seek :clear @21:49:39.84 ArS.coast @21:49:39.89 LyS1.coast @21:49:39.93 LyS2.coast @21:49:39.98 AirS.coast @21:49:40.03 TV.coast @21:49:40.09 SP.coast @21:49:40.14 SC.coast @21:49:40.19 PV.coast @21:49:40.24 PinPort.coast @21:49:40.42 Gate.power :motor,:OFF @21:49:40.44 Gate.power :main,:OFF @21:49:40.46 Gate.power :core,:OFF @21:49:40.48 Gate.power :analytic1,:OFF @21:49:40.50 Gate.power :analytic2,:OFF @21:49:40.52 Gate.power :analytic3,:OFF @21:49:40.54 Safely stopped and ready to power off ##-- file closed 2018-08-27T02:01:27.685Z ##-- file opened 2018-08-27T02:01:27.687Z @22:00:28.39 -> showStatus @22:00:30.37 -> Cmd.loadCartridge @22:00:30.49 Gate.power :main,:ON,limit to 5000mA @22:00:30.50 Gate.power :analytic1,:ON,limit to 5000mA @22:00:30.52 Gate.power :analytic2,:ON,limit to 5000mA @22:00:30.55 Gate.power :analytic3,:ON,limit to 5000mA @22:00:30.69 Powering core bus @22:00:33.00 SPR Module 1, FW Rev. B, FPGA Rev. C @22:00:33.09 ArS.configure ArSconfig @22:00:33.19 LyS1.configure LyS1config @22:00:33.31 LyS2.configure LyS2config @22:00:33.42 AirS.configure AirSconfig @22:00:33.54 TV.configure TVconfig @22:00:33.66 SP.configure SPconfig @22:00:33.78 SC.configure SCconfig @22:00:33.89 PV.configure PVconfig @22:00:34.01 PinPort.configure PPconfig @22:00:34.13 Sea.configure SPconfig @22:00:34.19 All elf controllers running firmware version 4.5 @22:00:34.21 Gate.power :motor,:ON,limit to 5000mA @22:00:34.33 ArS.reconfigure ArSconfig @22:00:34.61 ArS.home.jog 0 @22:00:34.76 PV.reconfigure PVconfig @22:00:34.84 TV.reconfigure TVconfig @22:00:34.92 LyS2.reconfigure LyS2config @22:00:34.97 LyS1.reconfigure LyS1config @22:00:35.03 PinPort.reconfigure PPconfig @22:00:35.07 AirS.reconfigure AirSconfig @22:00:35.22 PV.home.jog 0 @22:00:35.30 TV.home.jog 0 @22:00:35.33 PinPort.home.jog 0 @22:00:35.36 LyS2.home.jog 0 @22:00:35.40 LyS1.home.jog 0 @22:00:35.44 AirS.home.jog 0 @22:00:35.79 ArS.reconfigure HomeMagSyringe @22:00:35.97 TV.reconfigure HomeTV @22:00:36.05 PinPort.reconfigure HomePP @22:00:36.09 PV.reconfigure HomePV @22:00:36.12 LyS2.reconfigure HomeSyringe @22:00:36.16 ArS.jog -242 @22:00:36.32 PV.jog -1477 @22:00:36.34 TV.jog -546 @22:00:36.38 LyS2.jog -265 @22:00:36.41 PinPort.jog -110 @22:00:36.61 LyS1.reconfigure HomeSyringe @22:00:36.63 AirS.reconfigure HomeMagSyringe @22:00:36.71 LyS1.jog -265 @22:00:36.75 AirS.jog -321 @22:00:36.89 ArS.setRawPosition! -50 @22:00:36.91 PinPort.setRawPosition! 0 @22:00:36.97 ArS.seek :home @22:00:37.02 PinPort.seek :home @22:00:37.38 PinPort.reconfigure PPconfig @22:00:37.44 LyS2.setRawPosition! -50 @22:00:37.52 LyS2.seek :home @22:00:37.62 AirS.setRawPosition! -50 @22:00:37.66 ArS.reconfigure ArSconfig @22:00:37.68 LyS1.setRawPosition! -50 @22:00:37.77 AirS.seek :home @22:00:37.85 LyS1.seek :home @22:00:37.99 PV.setRawPosition! -50 @22:00:38.02 LyS2.reconfigure LyS2config @22:00:38.10 PV.seek :home @22:00:38.24 AirS.reconfigure AirSconfig @22:00:38.26 LyS1.reconfigure LyS1config @22:00:38.39 TV.setRawPosition! 0 @22:00:38.45 TV.seek :home @22:00:38.55 TV.reconfigure TVconfig @22:00:38.57 PV.reconfigure PVconfig @22:00:38.83 TV.seek :clear @22:00:40.45 TV.coast @22:00:40.50 SC.reconfigure HomeSC @22:00:40.56 SC.home.jog 0 @22:00:41.32 SC.reconfigure SCconfig @22:00:41.38 SC.seek 41 @22:00:41.48 Selecting Cartridge 40 @22:00:41.78 SC.seek 40 @22:00:42.30 LyS2.seek :locked @22:00:42.42 PV.seek :locked @22:00:42.55 AirS.seek :locked @22:00:46.63 Powering cartridge bus @22:00:48.76 Gate.setIR 1750,5000,0,6 @22:00:48.88 Gate.setIR 0,5000,0,6 @22:00:48.93 Loaded Lyse-n-Go Cartridge @22:00:48.94 PuckHeater.configure HeatConfig @22:00:49.00 Gate.power :heat,:ON,limit to 5000mA @22:01:09.11 -> Cmd.startFiltering @22:01:09.34 Duration of filtering limited to 1:05:00 @22:01:09.59 Priming sample loop w/100ml, bypass w/10ml @22:01:09.66 SP.setPosition! 0ml @22:01:09.71 Intake.open @22:01:09.73 Exhaust.open @22:01:10.58 Sea @-42.9psia @22:01:10.59 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @22:01:16.37 Vacuum tare is 24% of full scale @22:01:16.39 PB.open @22:01:16.42 Exhaust.close @22:01:17.25 Sea @-42.9psia @22:01:17.33 SP.reconfigure SPprime @22:01:17.40 SP.seek 10ml,1:21 @22:01:28.25 SP.setPosition! 0ml @22:01:28.29 PB.close @22:01:28.32 Exhaust.open @22:01:29.16 Sea @-42.9psia @22:01:29.24 SP.reconfigure SPprime @22:01:29.31 SP.seek 100ml,8:51 @22:03:23.71 Intake.close @22:03:24.48 Pressurizing cartridge to 26psi (try #1 of 3) @22:03:24.49 SP.setPosition! 0ml @22:03:24.54 PB.open @22:03:25.33 TV.seek :crack @22:03:26.12 TV.seek :clear @22:03:26.92 Intake.open @22:03:28.38 PB.close @22:03:29.10 Exhaust.close @22:03:29.86 Sea @-42.9psia @22:03:29.94 SP.reconfigure SPcharge @22:03:30.00 SP.seek 2.3ml,42 seconds @22:03:40.56 Cartridge pressurized with 0.521ml to 26.2psi @22:03:40.94 delay 3 seconds @22:03:44.01 Cartridge at 24.2psi 3 seconds later @22:03:44.09 TV.seek :cartridge @22:03:45.91 TV.seek :clear @22:03:47.72 Sampling 1000.0ml @22:03:47.73 SP.setPosition! 0ml @22:03:47.78 Exhaust.open @22:03:48.56 Sea @-42.9psia @22:03:48.65 SP.reconfigure SPsample @22:03:48.71 SP.seek 1000ml,1:23:53 @22:03:49.67 Cartridge 40 sampling 0/1000ml at 0.17ml/s with 3.9psi vacuum @22:04:49.68 Cartridge 40 sampling 32/1000ml at 0.54ml/s with 24.3psi vacuum @22:05:49.68 Cartridge 40 sampling 63/1000ml at 0.52ml/s with 24.0psi vacuum @22:06:49.68 Cartridge 40 sampling 93/1000ml at 0.50ml/s with 20.8psi vacuum @22:07:49.68 Cartridge 40 sampling 122/1000ml at 0.48ml/s with 22.6psi vacuum @22:08:49.68 Cartridge 40 sampling 149/1000ml at 0.40ml/s with 21.8psi vacuum @22:09:49.68 Cartridge 40 sampling 174/1000ml at 0.35ml/s with 21.9psi vacuum @22:10:49.68 Cartridge 40 sampling 194/1000ml at 0.34ml/s with 23.7psi vacuum @22:11:49.68 Cartridge 40 sampling 211/1000ml at 0.24ml/s with 25.1psi vacuum @22:12:49.68 Cartridge 40 sampling 225/1000ml at 0.20ml/s with 22.2psi vacuum @22:13:49.68 Cartridge 40 sampling 237/1000ml at 0.20ml/s with 26.3psi vacuum @22:14:44.46 Flow averaged 0.38ml/s over 10:52 @22:14:44.48 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @22:14:45.11 Relieved excess vacuum in 5.3 seconds @22:14:45.13 SP.seek 1000ml,1:03:10 @22:14:49.75 Cartridge 40 sampling 249/1000ml at 0.20ml/s with 26.0psi vacuum @22:14:56.72 Flow estimate reduced to 0.31ml/s @22:14:56.75 Waiting up to 14 seconds for vacuum to reduce from 28.3 to 18.0psi @22:14:57.37 Relieved excess vacuum in 7.5 seconds @22:14:57.65 SP.seek 1000ml,1:02:58 @22:14:59.90 Flow estimate reduced to 0.24ml/s @22:14:59.93 Waiting up to 11 seconds for vacuum to reduce from 27.0 to 18.0psi @22:15:00.55 Relieved excess vacuum in 2.8 seconds @22:15:00.57 SP.seek 1000ml,1:02:57 @22:15:10.57 Flow estimate reduced to 0.20ml/s @22:15:10.59 Intake.close @22:15:10.65 Exhaust.close @22:15:11.43 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @22:15:11.54 Sampled 253.0ml @22:15:11.62 TV.seek :bypass @22:15:13.23 TV.seek :clear @22:15:15.43 -> Cmd.startProcessing @22:15:17.22 SPR.command \"read_sequences\" @22:15:17.25 SPR.command \"stats_on\" @22:15:17.29 SPR.command \"start 0 1\" @22:15:19.30 Begun log /var/log/esp/spr18aug2622h.spr @22:15:20.10 Handoff.select :waste @22:15:20.22 ArS.seek empty @22:15:37.93 PinPort.seek :cartridge @22:15:43.64 LyS1.seek 1350ul @22:15:49.59 PV.seek :reagent @22:15:50.42 AirS.seek :tVent @22:15:52.49 delay 2 seconds @22:15:54.52 AirS.reconfigure EvacAirS @22:15:54.57 AirS.seek :evac @22:16:16.89 delay 15 seconds @22:16:31.92 AirS.reconfigure AirSconfig @22:16:31.97 AirS.seek :tVent @22:16:46.19 delay 2 seconds @22:16:48.22 AirS.reconfigure EvacAirS @22:16:48.27 AirS.seek :evac @22:17:10.57 delay 15 seconds @22:17:25.60 AirS.reconfigure AirSconfig @22:17:25.65 AirS.seek :tVent @22:17:39.87 delay 2 seconds @22:17:41.90 AirS.reconfigure EvacAirS @22:17:41.95 AirS.seek :evac @22:18:04.24 delay 15 seconds @22:18:19.27 AirS.reconfigure AirSconfig @22:18:19.33 AirS.seek :tVent @22:18:33.55 delay 2 seconds @22:18:35.59 AirS.reconfigure EvacAirS @22:18:35.64 AirS.seek :evac @22:18:57.94 delay 15 seconds @22:19:12.97 AirS.reconfigure AirSconfig @22:19:13.02 AirS.seek :tVent @22:19:27.24 delay 2 seconds @22:19:29.27 AirS.reconfigure EvacAirS @22:19:29.32 AirS.seek :evac @22:19:51.64 delay 15 seconds @22:20:06.66 delay 1:30 @22:21:36.69 AirS.reconfigure AirSconfig @22:21:36.74 AirS.seek :fVent @22:21:38.18 delay 15 seconds @22:21:53.20 LyS1.reconfigure SlowLyS1 @22:21:53.31 LyS1.seek 1050ul @22:22:23.34 delay 5 seconds @22:22:28.37 Handoff.select :blocked @22:22:28.53 LyS1.seek 450ul @22:23:28.49 delay 10 seconds @22:23:38.51 AirS.reconfigure SlowAirS @22:23:38.62 AirS.reconfigure to mix into 1.37806 parts LyS1 @22:23:39.03 AirS.seek evac @22:23:39.07 LyS1.seek 300ul @22:23:54.18 AirS.reconfigure AirSconfig @22:23:54.25 AirS.seek :full @22:24:08.46 LyS1.seek :empty @22:24:38.56 PuckHeater.seek 84.3C @22:25:46.23 PuckHeater.hold 15:00,84.3C @22:40:41.08 LyS1.reconfigure LyS1config @22:40:41.14 LyS1.seek 900ul @22:40:49.49 AirS.seek :tVent @22:40:49.57 delay 2 seconds @22:40:51.60 AirS.reconfigure HalfSpeedAirS @22:40:51.65 AirS.seek :evac @22:41:19.68 delay 15 seconds @22:41:34.71 AirS.reconfigure AirSconfig @22:41:34.76 AirS.seek :tVent @22:41:48.99 delay 2 seconds @22:41:51.02 AirS.reconfigure HalfSpeedAirS @22:41:51.07 AirS.seek :evac @22:42:18.84 delay 15 seconds @22:42:33.87 AirS.reconfigure AirSconfig @22:42:33.92 AirS.seek :tVent @22:42:48.15 delay 2 seconds @22:42:50.18 AirS.reconfigure HalfSpeedAirS @22:42:50.23 AirS.seek :evac @22:43:17.99 delay 15 seconds @22:43:33.01 delay 1:00 @22:44:33.03 Handoff.select :waste @22:44:33.13 delay 15 seconds @22:44:48.16 AirS.seek :fVent @22:44:50.35 delay 10 seconds @22:45:00.37 AirS.reconfigure AirSconfig @22:45:00.43 AirS.seek :evac @22:45:01.87 Lysis Recovered in LyS1 @22:45:01.88 Lysis Complete @22:45:01.89 Handoff.select :waste @22:45:01.92 LyS2.seek :full @22:45:02.01 LyS1.reconfigure DAmixLyS1 @22:45:02.06 LyS2.reconfigure DAmixLyS2 @22:45:02.46 LyS2.seek 1480ul @22:45:02.50 LyS1.seek 650ul @22:45:49.87 delay 5 seconds @22:45:54.89 Handoff.select :SPR @22:45:55.01 SPR.command \"start 2 14\" @22:45:55.95 Running Experiment 2 @22:45:55.96 delay 10 seconds @22:46:05.98 SPR.command \"start 2 0\" @22:46:09.32 Waiting up to 11:00 for SPR to enter state 1 @22:53:27.61 SPR.command \"hold_on\" @22:53:29.31 LyS2.seek 1130ul @22:53:29.35 LyS1.seek 300ul @22:54:35.55 delay 15 seconds @22:54:50.57 SPR.command \"hold_off\" @22:54:53.81 Waiting up to 25:00 for SPR to enter state 7 @23:06:30.75 Handoff.select :waste @23:06:30.87 LyS1.reconfigure LyS1config @23:06:30.93 LyS2.reconfigure LyS2config @23:06:30.99 Rep #1 SPR Analysis has Completed @23:06:31.68 Ended log /var/log/esp/spr18aug2622h.spr @23:06:31.69 SPR.command \"stats_off\" @23:06:33.71 -> Cmd.stop @23:06:33.80 Handoff.select :close @23:06:33.97 Gate.power :heat,:OFF @23:06:33.99 Gate.power :cartridge,:OFF @23:06:34.02 PV.seek :locked @23:06:34.92 PinPort.seek :clear @23:06:40.92 ArS.seek :clear @23:06:41.05 LyS1.seek :clear @23:06:41.18 LyS2.seek :clear @23:06:41.33 AirS.seek :clear @23:06:58.68 ArS.coast @23:06:58.72 LyS1.coast @23:06:58.77 LyS2.coast @23:06:58.82 AirS.coast @23:06:58.88 TV.coast @23:06:58.93 SP.coast @23:06:58.98 SC.coast @23:06:59.03 PV.coast @23:06:59.08 PinPort.coast @23:06:59.26 Gate.power :motor,:OFF @23:06:59.28 Gate.power :main,:OFF @23:06:59.30 Gate.power :core,:OFF @23:06:59.32 Gate.power :analytic1,:OFF @23:06:59.34 Gate.power :analytic2,:OFF @23:06:59.36 Gate.power :analytic3,:OFF @23:06:59.38 Safely stopped and ready to power off ##-- file closed 2018-08-27T03:18:53.117Z ##-- file opened 2018-08-27T03:18:53.129Z @23:17:46.60 -> showStatus @23:17:48.61 -> Cmd.loadCartridge @23:17:48.73 Gate.power :main,:ON,limit to 5000mA @23:17:48.75 Gate.power :analytic1,:ON,limit to 5000mA @23:17:48.77 Gate.power :analytic2,:ON,limit to 5000mA @23:17:48.79 Gate.power :analytic3,:ON,limit to 5000mA @23:17:48.93 Powering core bus @23:17:51.24 SPR Module 1, FW Rev. B, FPGA Rev. C @23:17:51.34 ArS.configure ArSconfig @23:17:51.44 LyS1.configure LyS1config @23:17:51.57 LyS2.configure LyS2config @23:17:51.67 AirS.configure AirSconfig @23:17:51.79 TV.configure TVconfig @23:17:51.92 SP.configure SPconfig @23:17:52.03 SC.configure SCconfig @23:17:52.15 PV.configure PVconfig @23:17:52.27 PinPort.configure PPconfig @23:17:52.39 Sea.configure SPconfig @23:17:52.44 All elf controllers running firmware version 4.5 @23:17:52.46 Gate.power :motor,:ON,limit to 5000mA @23:17:52.99 PV.reconfigure PVconfig @23:17:53.02 TV.reconfigure TVconfig @23:17:53.05 PinPort.reconfigure PPconfig @23:17:53.10 AirS.reconfigure AirSconfig @23:17:53.13 LyS1.reconfigure LyS1config @23:17:53.16 ArS.reconfigure ArSconfig @23:17:53.20 LyS2.reconfigure LyS2config @23:17:53.41 PV.home.jog 0 @23:17:53.46 TV.home.jog 0 @23:17:53.48 PinPort.home.jog 0 @23:17:53.51 AirS.home.jog 0 @23:17:53.53 LyS1.home.jog 0 @23:17:53.56 LyS2.home.jog 0 @23:17:53.60 ArS.home.jog 0 @23:17:54.12 TV.reconfigure HomeTV @23:17:54.19 PV.reconfigure HomePV @23:17:54.28 LyS1.reconfigure HomeSyringe @23:17:54.34 PinPort.reconfigure HomePP @23:17:54.51 TV.jog -545 @23:17:54.54 PinPort.jog -109 @23:17:54.57 PV.jog -1485 @23:17:54.60 LyS1.jog -266 @23:17:54.83 LyS2.reconfigure HomeSyringe @23:17:54.85 ArS.reconfigure HomeMagSyringe @23:17:54.93 AirS.reconfigure HomeMagSyringe @23:17:55.07 LyS2.jog -264 @23:17:55.11 AirS.jog -330 @23:17:55.15 ArS.jog -241 @23:17:55.32 LyS1.setRawPosition! -50 @23:17:55.34 PinPort.setRawPosition! 0 @23:17:55.43 LyS1.seek :home @23:17:55.45 PinPort.seek :home @23:17:55.89 PV.setRawPosition! -50 @23:17:55.92 LyS2.setRawPosition! -50 @23:17:55.94 ArS.setRawPosition! -50 @23:17:55.98 PinPort.reconfigure PPconfig @23:17:56.00 AirS.setRawPosition! -50 @23:17:56.17 PV.seek :home @23:17:56.20 LyS2.seek :home @23:17:56.24 ArS.seek :home @23:17:56.27 AirS.seek :home @23:17:56.52 TV.setRawPosition! 0 @23:17:56.54 LyS1.reconfigure LyS1config @23:17:56.63 TV.seek :home @23:17:56.82 TV.reconfigure TVconfig @23:17:56.85 AirS.reconfigure AirSconfig @23:17:56.88 ArS.reconfigure ArSconfig @23:17:56.91 PV.reconfigure PVconfig @23:17:56.94 LyS2.reconfigure LyS2config @23:17:57.60 TV.seek :clear @23:17:59.22 TV.coast @23:17:59.27 SC.reconfigure HomeSC @23:17:59.34 SC.home.jog 0 @23:18:00.09 SC.reconfigure SCconfig @23:18:00.15 SC.seek 40 @23:18:00.26 Selecting Cartridge 39 @23:18:00.57 SC.seek 39 @23:18:01.07 LyS2.seek :locked @23:18:01.19 PV.seek :locked @23:18:01.33 AirS.seek :locked @23:18:05.41 Powering cartridge bus @23:18:07.53 Gate.setIR 1750,5000,0,6 @23:18:07.65 Gate.setIR 0,5000,0,6 @23:18:07.69 Loaded Archive Cartridge @23:18:07.70 Gate.power :cartridge,:OFF @23:18:26.92 -> Cmd.startFiltering @23:18:27.15 Duration of filtering limited to 1:05:00 @23:18:27.40 Priming sample loop w/100ml, bypass w/10ml @23:18:27.48 SP.setPosition! 0ml @23:18:27.52 Intake.open @23:18:27.54 Exhaust.open @23:18:28.38 Sea @-42.9psia @23:18:28.40 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @23:18:34.18 Vacuum tare is 24% of full scale @23:18:34.19 PB.open @23:18:34.22 Exhaust.close @23:18:35.02 Sea @-42.9psia @23:18:35.10 SP.reconfigure SPprime @23:18:35.17 SP.seek 10ml,1:21 @23:18:46.02 SP.setPosition! 0ml @23:18:46.07 PB.close @23:18:46.09 Exhaust.open @23:18:46.91 Sea @-42.9psia @23:18:46.99 SP.reconfigure SPprime @23:18:47.05 SP.seek 100ml,8:51 @23:20:41.14 Intake.close @23:20:41.88 Pressurizing cartridge to 26psi (try #1 of 3) @23:20:41.90 SP.setPosition! 0ml @23:20:41.95 PB.open @23:20:42.73 TV.seek :crack @23:20:43.53 TV.seek :clear @23:20:44.32 Intake.open @23:20:45.80 PB.close @23:20:46.52 Exhaust.close @23:20:47.29 Sea @-42.9psia @23:20:47.38 SP.reconfigure SPcharge @23:20:47.44 SP.seek 2.3ml,42 seconds @23:20:54.63 Cartridge pressurized with 0.239ml to 26.1psi @23:20:54.86 delay 3 seconds @23:20:57.93 Cartridge at 21.4psi 3 seconds later @23:20:58.01 TV.seek :cartridge @23:20:59.83 TV.seek :clear @23:21:01.63 Sampling 1000.0ml @23:21:01.65 SP.setPosition! 0ml @23:21:01.70 Exhaust.open @23:21:02.50 Sea @-42.9psia @23:21:02.59 SP.reconfigure SPsample @23:21:02.66 SP.seek 1000ml,1:23:53 @23:21:03.61 Cartridge 39 sampling 0/1000ml at 0.17ml/s with 6.4psi vacuum @23:22:03.62 Cartridge 39 sampling 22/1000ml at 0.30ml/s with 17.7psi vacuum @23:23:03.62 Cartridge 39 sampling 39/1000ml at 0.22ml/s with 20.7psi vacuum @23:24:03.62 Cartridge 39 sampling 52/1000ml at 0.21ml/s with 22.7psi vacuum @23:24:22.29 Flow averaged 0.29ml/s over 3:12 @23:24:22.32 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @23:24:22.93 Relieved excess vacuum in 9.2 seconds @23:24:22.96 SP.seek 1000ml,1:19:15 @23:24:31.42 Flow estimate reduced to 0.23ml/s @23:24:31.44 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @23:24:32.06 Relieved excess vacuum in 6 seconds @23:24:32.09 SP.seek 1000ml,1:19:07 @23:24:37.41 Flow estimate reduced to 0.19ml/s @23:24:37.43 Intake.close @23:24:37.46 Exhaust.close @23:24:38.26 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @23:24:38.37 Sampled 58.0ml @23:24:38.45 TV.seek :bypass @23:24:40.07 TV.seek :clear @23:24:42.37 -> Cmd.startProcessing @23:24:42.63 PV.seek :reagent @23:24:43.47 Sample loop closed. Ready to Deliver Reagents @23:24:43.49 ArS.seek :sealed @23:24:48.01 ArS.seek :evac @23:25:01.93 delay 20 seconds @23:25:21.97 ArS.seek :clear @23:25:39.68 RNAlater soak for 10:00 @23:35:39.71 ArS.seek :tVent @23:35:42.28 delay 1 second @23:35:43.31 ArS.seek :evac @23:35:58.92 delay 10 seconds @23:36:08.96 Sample preserved and evacuated @23:36:10.13 -> Cmd.stop @23:36:10.28 PV.seek :locked @23:36:11.17 PinPort.seek :clear @23:36:11.32 ArS.seek :clear @23:36:11.50 LyS2.seek :clear @23:36:11.63 AirS.seek :clear @23:36:29.07 ArS.coast @23:36:29.12 LyS1.coast @23:36:29.17 LyS2.coast @23:36:29.22 AirS.coast @23:36:29.27 TV.coast @23:36:29.32 SP.coast @23:36:29.37 SC.coast @23:36:29.42 PV.coast @23:36:29.47 PinPort.coast @23:36:29.64 Gate.power :motor,:OFF @23:36:29.66 Gate.power :main,:OFF @23:36:29.68 Gate.power :core,:OFF @23:36:29.70 Gate.power :analytic1,:OFF @23:36:29.72 Gate.power :analytic2,:OFF @23:36:29.74 Gate.power :analytic3,:OFF @23:36:29.77 Safely stopped and ready to power off ##-- file closed 2018-08-27T12:01:04.698Z