##-- file opened 2018-08-04T16:58:44.404Z @09:58:34.16 -> showStatus @09:58:35.74 -> Cmd.loadCartridge @09:58:35.86 Gate.power :main,:ON,limit to 5000mA @09:58:35.87 Gate.power :analytic1,:ON,limit to 5000mA @09:58:35.89 Gate.power :analytic2,:ON,limit to 5000mA @09:58:35.91 Gate.power :analytic3,:ON,limit to 5000mA @09:58:36.05 Powering core bus @09:58:38.35 SPR Module 1, FW Rev. B, FPGA Rev. C @09:58:38.44 ArS.configure ArSconfig @09:58:38.54 LyS1.configure LyS1config @09:58:38.66 LyS2.configure LyS2config @09:58:38.76 AirS.configure AirSconfig @09:58:38.93 TV.configure TVconfig @09:58:39.06 SP.configure SPconfig @09:58:39.18 SC.configure SCconfig @09:58:39.30 PV.configure PVconfig @09:58:39.42 PinPort.configure PPconfig @09:58:39.53 Sea.configure SPconfig @09:58:39.59 All elf controllers running firmware version 4.46 @09:58:39.60 Gate.power :motor,:ON,limit to 5000mA @09:58:40.10 TV.reconfigure TVconfig @09:58:40.17 AirS.reconfigure AirSconfig @09:58:40.21 PV.reconfigure PVconfig @09:58:40.25 LyS2.reconfigure LyS2config @09:58:40.28 LyS1.reconfigure LyS1config @09:58:40.33 PinPort.reconfigure PPconfig @09:58:40.36 ArS.reconfigure ArSconfig @09:58:40.56 TV.home.jog 0 @09:58:40.60 AirS.home.jog 0 @09:58:40.62 PV.home.jog 0 @09:58:40.65 LyS2.home.jog 0 @09:58:40.68 LyS1.home.jog 0 @09:58:40.70 PinPort.home.jog 0 @09:58:40.76 ArS.home.jog 0 @09:58:41.28 PV.reconfigure HomePV @09:58:41.32 TV.reconfigure HomeTV @09:58:41.35 AirS.reconfigure HomeMagSyringe @09:58:41.40 PinPort.reconfigure HomePP @09:58:41.60 TV.jog -557 @09:58:41.66 PV.jog -1505 @09:58:41.69 PinPort.jog -109 @09:58:41.72 AirS.jog -337 @09:58:41.93 LyS1.reconfigure HomeSyringe @09:58:41.95 ArS.reconfigure HomeMagSyringe @09:58:41.98 LyS2.reconfigure HomeSyringe @09:58:42.12 ArS.jog -286 @09:58:42.14 LyS2.jog -274 @09:58:42.17 LyS1.jog -284 @09:58:42.33 PinPort.setRawPosition! 0 @09:58:42.37 PinPort.seek :home @09:58:42.47 PinPort.reconfigure PPconfig @09:58:42.49 AirS.setRawPosition! -50 @09:58:42.56 AirS.seek :home @09:58:42.92 LyS2.setRawPosition! -50 @09:58:42.95 LyS1.setRawPosition! -50 @09:58:42.97 ArS.setRawPosition! -50 @09:58:43.05 LyS2.seek :home @09:58:43.12 LyS1.seek :home @09:58:43.16 ArS.seek :home @09:58:43.35 PV.setRawPosition! -50 @09:58:43.37 AirS.reconfigure AirSconfig @09:58:43.45 PV.seek :home @09:58:43.65 TV.setRawPosition! 0 @09:58:43.69 LyS1.reconfigure LyS1config @09:58:43.75 ArS.reconfigure ArSconfig @09:58:43.79 LyS2.reconfigure LyS2config @09:58:43.82 TV.seek :home @09:58:44.05 TV.reconfigure TVconfig @09:58:44.07 PV.reconfigure PVconfig @09:58:44.48 TV.seek :clear @09:58:46.11 TV.coast @09:58:46.16 SC.reconfigure HomeSC @09:58:46.22 SC.home.jog 0 @09:58:46.97 SC.reconfigure SCconfig @09:58:47.03 SC.seek 48 @09:58:47.12 Selecting Cartridge 47 @09:58:47.43 SC.seek 47 @09:58:47.92 LyS2.seek :locked @09:58:48.05 PV.seek :locked @09:58:48.18 AirS.seek :locked @09:58:52.26 Powering cartridge bus @09:58:54.38 Gate.setIR 1750,5000,0,6 @09:58:54.51 Gate.setIR 0,5000,0,6 @09:58:54.54 Loaded Archive Cartridge @09:58:54.55 Gate.power :cartridge,:OFF @09:59:14.34 -> Cmd.startFiltering @09:59:14.56 Duration of filtering limited to 1:05:00 @09:59:14.83 Priming sample loop w/100ml, bypass w/10ml @09:59:14.91 SP.setPosition! 0ml @09:59:14.95 Intake.open @09:59:14.97 Exhaust.open @09:59:15.79 Sea @-42.9psia @09:59:15.80 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @09:59:21.34 Vacuum tare is 23% of full scale @09:59:21.36 PB.open @09:59:21.64 Exhaust.close @09:59:22.47 Sea @-42.9psia @09:59:22.56 SP.reconfigure SPprime @09:59:22.62 SP.seek 10ml,1:21 @09:59:33.51 SP.setPosition! 0ml @09:59:33.55 PB.close @09:59:33.58 Exhaust.open @09:59:34.40 Sea @-42.9psia @09:59:34.48 SP.reconfigure SPprime @09:59:34.55 SP.seek 100ml,8:51 @10:01:29.02 Pressurizing cartridge to 26psi @10:01:29.03 Intake.close @10:01:29.79 SP.setPosition! 0ml @10:01:29.83 PB.open @10:01:30.55 TV.seek :crack @10:01:31.34 TV.seek :clear @10:01:32.14 Intake.open @10:01:33.62 PB.close @10:01:34.32 Exhaust.close @10:01:35.07 Sea @-42.9psia @10:01:35.15 SP.reconfigure SPcharge @10:01:35.22 SP.seek 7.5ml,1:08 @10:01:41.02 Cartridge pressurized with 0.154ml to 26.0psi @10:01:41.25 delay 3 seconds @10:01:44.32 Cartridge at 20.1psi 3 seconds later @10:01:44.40 TV.seek :cartridge @10:01:46.22 TV.seek :clear @10:01:48.03 Sampling 1000.0ml @10:01:48.04 SP.setPosition! 0ml @10:01:48.09 Exhaust.open @10:01:48.86 Sea @-42.9psia @10:01:48.95 SP.reconfigure SPsample @10:01:49.02 SP.seek 1000ml,1:23:53 @10:01:49.97 Cartridge 47 sampling 0/1000ml at 0.17ml/s with 8.7psi vacuum @10:02:49.98 Cartridge 47 sampling 21/1000ml at 0.33ml/s with 22.0psi vacuum @10:03:49.98 Cartridge 47 sampling 41/1000ml at 0.33ml/s with 17.3psi vacuum @10:04:49.98 Cartridge 47 sampling 60/1000ml at 0.30ml/s with 26.4psi vacuum @10:05:49.98 Cartridge 47 sampling 80/1000ml at 0.36ml/s with 22.7psi vacuum @10:06:49.98 Cartridge 47 sampling 99/1000ml at 0.30ml/s with 22.2psi vacuum @10:07:49.98 Cartridge 47 sampling 117/1000ml at 0.27ml/s with 19.7psi vacuum @10:08:49.98 Cartridge 47 sampling 135/1000ml at 0.26ml/s with 18.7psi vacuum @10:09:49.98 Cartridge 47 sampling 153/1000ml at 0.26ml/s with 21.3psi vacuum @10:10:49.98 Cartridge 47 sampling 171/1000ml at 0.28ml/s with 22.3psi vacuum @10:11:49.98 Cartridge 47 sampling 188/1000ml at 0.28ml/s with 23.9psi vacuum @10:12:49.98 Cartridge 47 sampling 205/1000ml at 0.29ml/s with 25.7psi vacuum @10:13:49.98 Cartridge 47 sampling 221/1000ml at 0.29ml/s with 25.3psi vacuum @10:14:49.98 Cartridge 47 sampling 238/1000ml at 0.28ml/s with 25.4psi vacuum @10:15:49.98 Cartridge 47 sampling 254/1000ml at 0.25ml/s with 25.3psi vacuum @10:16:49.98 Cartridge 47 sampling 270/1000ml at 0.30ml/s with 22.4psi vacuum @10:17:49.98 Cartridge 47 sampling 285/1000ml at 0.26ml/s with 19.6psi vacuum @10:18:50.24 Cartridge 47 sampling 300/1000ml at 0.28ml/s with 21.0psi vacuum @10:19:49.98 Cartridge 47 sampling 315/1000ml at 0.26ml/s with 18.5psi vacuum @10:20:49.99 Cartridge 47 sampling 330/1000ml at 0.23ml/s with 23.2psi vacuum @10:21:49.98 Cartridge 47 sampling 345/1000ml at 0.25ml/s with 22.7psi vacuum @10:22:49.98 Cartridge 47 sampling 359/1000ml at 0.23ml/s with 22.2psi vacuum @10:23:49.99 Cartridge 47 sampling 373/1000ml at 0.22ml/s with 27.0psi vacuum @10:24:49.98 Cartridge 47 sampling 387/1000ml at 0.23ml/s with 23.7psi vacuum @10:25:49.98 Cartridge 47 sampling 401/1000ml at 0.22ml/s with 24.6psi vacuum @10:26:49.98 Cartridge 47 sampling 415/1000ml at 0.22ml/s with 24.7psi vacuum @10:27:49.98 Cartridge 47 sampling 428/1000ml at 0.22ml/s with 24.9psi vacuum @10:28:49.98 Cartridge 47 sampling 442/1000ml at 0.21ml/s with 17.6psi vacuum @10:29:49.98 Cartridge 47 sampling 455/1000ml at 0.21ml/s with 18.7psi vacuum @10:30:49.98 Cartridge 47 sampling 468/1000ml at 0.20ml/s with 22.6psi vacuum @10:31:49.98 Cartridge 47 sampling 480/1000ml at 0.20ml/s with 25.4psi vacuum @10:32:49.98 Cartridge 47 sampling 493/1000ml at 0.20ml/s with 24.7psi vacuum @10:33:49.98 Cartridge 47 sampling 506/1000ml at 0.22ml/s with 24.5psi vacuum @10:34:49.98 Cartridge 47 sampling 518/1000ml at 0.20ml/s with 25.3psi vacuum @10:35:49.98 Cartridge 47 sampling 531/1000ml at 0.21ml/s with 22.9psi vacuum @10:36:49.98 Cartridge 47 sampling 544/1000ml at 0.20ml/s with 26.0psi vacuum @10:37:49.98 Cartridge 47 sampling 556/1000ml at 0.20ml/s with 23.9psi vacuum @10:38:49.98 Cartridge 47 sampling 568/1000ml at 0.20ml/s with 24.6psi vacuum @10:39:49.98 Cartridge 47 sampling 581/1000ml at 0.22ml/s with 24.0psi vacuum @10:40:49.98 Cartridge 47 sampling 593/1000ml at 0.20ml/s with 25.9psi vacuum @10:41:49.98 Cartridge 47 sampling 605/1000ml at 0.22ml/s with 22.9psi vacuum @10:42:49.98 Cartridge 47 sampling 618/1000ml at 0.20ml/s with 24.9psi vacuum @10:43:49.98 Cartridge 47 sampling 630/1000ml at 0.20ml/s with 27.4psi vacuum @10:44:49.98 Cartridge 47 sampling 642/1000ml at 0.20ml/s with 27.8psi vacuum @10:45:49.98 Cartridge 47 sampling 654/1000ml at 0.20ml/s with 30.1psi vacuum @10:46:49.98 Cartridge 47 sampling 666/1000ml at 0.20ml/s with 21.2psi vacuum @10:47:49.98 Cartridge 47 sampling 678/1000ml at 0.20ml/s with 24.9psi vacuum @10:48:49.97 Cartridge 47 sampling 690/1000ml at 0.20ml/s with 25.2psi vacuum @10:49:49.98 Cartridge 47 sampling 703/1000ml at 0.20ml/s with 24.3psi vacuum @10:50:49.98 Cartridge 47 sampling 715/1000ml at 0.20ml/s with 25.0psi vacuum @10:50:52.90 Flow averaged 0.24ml/s over 49:02 @10:50:52.92 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi @10:50:53.54 Relieved excess vacuum in 3.5 seconds @10:50:53.56 SP.seek 1000ml,24:14 @10:51:49.98 Cartridge 47 sampling 727/1000ml at 0.20ml/s with 23.8psi vacuum @10:52:49.98 Cartridge 47 sampling 739/1000ml at 0.20ml/s with 24.7psi vacuum @10:53:50.23 Cartridge 47 sampling 751/1000ml at 0.20ml/s with 28.4psi vacuum @10:53:58.81 Flow averaged 0.21ml/s over 2:57 @10:53:58.83 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi @10:53:59.45 Relieved excess vacuum in 9.1 seconds @10:53:59.47 SP.seek 1000ml,21:08 @10:54:49.98 Cartridge 47 sampling 763/1000ml at 0.20ml/s with 28.9psi vacuum @10:55:34.66 Flow averaged 0.21ml/s over 1:31 @10:55:34.68 Waiting up to 8 seconds for vacuum to reduce from 26.0 to 18.0psi @10:55:35.31 Relieved excess vacuum in 5.2 seconds @10:55:35.33 SP.seek 1000ml,19:32 @10:55:49.97 Cartridge 47 sampling 775/1000ml at 0.20ml/s with 28.4psi vacuum @10:56:49.98 Cartridge 47 sampling 787/1000ml at 0.20ml/s with 30.9psi vacuum @10:57:40.61 Flow averaged 0.20ml/s over 2:05 @10:57:40.63 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @10:57:41.26 Relieved excess vacuum in 1.1 seconds @10:57:41.28 SP.seek 1000ml,17:26 @10:57:49.98 Cartridge 47 sampling 799/1000ml at 0.20ml/s with 25.5psi vacuum @10:58:19.83 Flow averaged 0.27ml/s over 29 seconds @10:58:19.85 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @10:58:20.47 Relieved excess vacuum in 10.4 seconds @10:58:20.49 SP.seek 1000ml,16:47 @10:58:49.97 Cartridge 47 sampling 811/1000ml at 0.20ml/s with 28.7psi vacuum @10:59:17.94 Flow averaged 0.23ml/s over 50 seconds @10:59:17.96 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @10:59:18.59 Relieved excess vacuum in 8.5 seconds @10:59:18.61 SP.seek 1000ml,15:50 @10:59:49.98 Cartridge 47 sampling 822/1000ml at 0.20ml/s with 29.7psi vacuum @11:00:05.02 Flow averaged 0.22ml/s over 42 seconds @11:00:05.04 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @11:00:05.66 Relieved excess vacuum in 5.6 seconds @11:00:05.68 SP.seek 1000ml,15:04 @11:00:49.97 Cartridge 47 sampling 834/1000ml at 0.20ml/s with 29.2psi vacuum @11:00:52.07 Flow averaged 0.21ml/s over 45 seconds @11:00:52.09 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @11:00:52.72 Relieved excess vacuum in 2.7 seconds @11:00:52.74 SP.seek 1000ml,14:17 @11:01:49.98 Cartridge 47 sampling 846/1000ml at 0.20ml/s with 26.2psi vacuum @11:01:55.02 Flow averaged 0.22ml/s over 58 seconds @11:01:55.04 Waiting up to 8 seconds for vacuum to reduce from 26.0 to 18.0psi @11:01:55.67 Relieved excess vacuum in 5.6 seconds @11:01:55.69 SP.seek 1000ml,13:15 @11:02:08.98 Flow averaged 0.20ml/s over 14 seconds @11:02:08.99 Intake.close @11:02:09.04 Exhaust.close @11:02:09.83 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:02:09.92 Sampled 849.7ml @11:02:10.01 TV.seek :bypass @11:02:11.62 TV.seek :clear @11:02:13.84 -> Cmd.startProcessing @11:02:14.09 PV.seek :reagent @11:02:14.94 Sample loop closed. Ready to Deliver Reagents @11:02:14.95 ArS.seek :sealed @11:02:19.22 ArS.seek :evac @11:02:33.17 delay 20 seconds @11:02:53.20 ArS.seek :clear @11:03:10.94 RNAlater soak for 10:00 @11:13:10.96 ArS.seek :tVent @11:13:13.54 delay 1 second @11:13:14.57 ArS.seek :evac @11:13:30.19 delay 10 seconds @11:13:40.22 Sample preserved and evacuated @11:13:41.41 -> Cmd.stop @11:13:41.56 PV.seek :locked @11:13:42.46 PinPort.seek :clear @11:13:42.60 ArS.seek :clear @11:13:42.78 LyS2.seek :clear @11:13:42.91 AirS.seek :clear @11:14:00.35 ArS.coast @11:14:00.40 LyS1.coast @11:14:00.45 LyS2.coast @11:14:00.50 AirS.coast @11:14:00.55 TV.coast @11:14:00.60 SP.coast @11:14:00.65 SC.coast @11:14:00.71 PV.coast @11:14:00.76 PinPort.coast @11:14:00.94 Gate.power :motor,:OFF @11:14:00.95 Gate.power :main,:OFF @11:14:00.98 Gate.power :core,:OFF @11:14:01.00 Gate.power :analytic1,:OFF @11:14:01.02 Gate.power :analytic2,:OFF @11:14:01.04 Gate.power :analytic3,:OFF @11:14:01.06 Safely stopped and ready to power off ##-- file closed 2018-08-04T18:19:35.654Z ##-- file opened 2018-08-04T18:19:35.657Z @11:19:17.45 -> showStatus @11:19:19.46 -> Cmd.loadCartridge @11:19:19.58 Gate.power :main,:ON,limit to 5000mA @11:19:19.60 Gate.power :analytic1,:ON,limit to 5000mA @11:19:19.62 Gate.power :analytic2,:ON,limit to 5000mA @11:19:19.64 Gate.power :analytic3,:ON,limit to 5000mA @11:19:19.79 Powering core bus @11:19:22.08 SPR Module 1, FW Rev. B, FPGA Rev. C @11:19:22.18 ArS.configure ArSconfig @11:19:22.28 LyS1.configure LyS1config @11:19:22.40 LyS2.configure LyS2config @11:19:22.51 AirS.configure AirSconfig @11:19:22.63 TV.configure TVconfig @11:19:22.75 SP.configure SPconfig @11:19:22.87 SC.configure SCconfig @11:19:22.99 PV.configure PVconfig @11:19:23.11 PinPort.configure PPconfig @11:19:23.23 Sea.configure SPconfig @11:19:23.29 All elf controllers running firmware version 4.46 @11:19:23.31 Gate.power :motor,:ON,limit to 5000mA @11:19:23.81 PV.reconfigure PVconfig @11:19:23.87 TV.reconfigure TVconfig @11:19:23.90 LyS2.reconfigure LyS2config @11:19:23.94 ArS.reconfigure ArSconfig @11:19:23.97 PinPort.reconfigure PPconfig @11:19:24.02 AirS.reconfigure AirSconfig @11:19:24.05 LyS1.reconfigure LyS1config @11:19:24.24 PV.home.jog 0 @11:19:24.29 TV.home.jog 0 @11:19:24.31 ArS.home.jog 0 @11:19:24.34 LyS2.home.jog 0 @11:19:24.37 AirS.home.jog 0 @11:19:24.41 LyS1.home.jog 0 @11:19:24.46 PinPort.home.jog 0 @11:19:25.14 LyS2.reconfigure HomeSyringe @11:19:25.19 PinPort.reconfigure HomePP @11:19:25.24 TV.reconfigure HomeTV @11:19:25.27 AirS.reconfigure HomeMagSyringe @11:19:25.31 ArS.reconfigure HomeMagSyringe @11:19:25.34 PV.reconfigure HomePV @11:19:25.54 LyS2.jog -277 @11:19:25.58 TV.jog -554 @11:19:25.62 PinPort.jog -107 @11:19:25.65 PV.jog -1505 @11:19:25.68 ArS.jog -290 @11:19:25.71 AirS.jog -340 @11:19:25.94 LyS1.reconfigure HomeSyringe @11:19:26.00 LyS1.jog -285 @11:19:26.11 PinPort.setRawPosition! 0 @11:19:26.16 PinPort.seek :home @11:19:26.24 PinPort.reconfigure PPconfig @11:19:26.33 LyS2.setRawPosition! -50 @11:19:26.38 LyS2.seek :home @11:19:26.72 AirS.setRawPosition! -50 @11:19:26.74 ArS.setRawPosition! -50 @11:19:26.76 LyS1.setRawPosition! -50 @11:19:26.85 AirS.seek :home @11:19:26.90 ArS.seek :home @11:19:26.97 LyS1.seek :home @11:19:27.17 PV.setRawPosition! -50 @11:19:27.19 LyS2.reconfigure LyS2config @11:19:27.28 PV.seek :home @11:19:27.40 AirS.reconfigure AirSconfig @11:19:27.43 LyS1.reconfigure LyS1config @11:19:27.46 ArS.reconfigure ArSconfig @11:19:27.68 TV.setRawPosition! 0 @11:19:27.70 PV.reconfigure PVconfig @11:19:27.79 TV.seek :home @11:19:27.89 TV.reconfigure TVconfig @11:19:28.12 TV.seek :clear @11:19:29.75 TV.coast @11:19:29.80 SC.reconfigure HomeSC @11:19:29.87 SC.home.jog 0 @11:19:30.63 SC.reconfigure SCconfig @11:19:30.69 SC.seek 47 @11:19:30.78 Selecting Cartridge 46 @11:19:31.09 SC.seek 46 @11:19:31.58 LyS2.seek :locked @11:19:31.71 PV.seek :locked @11:19:31.84 AirS.seek :locked @11:19:35.92 Powering cartridge bus @11:19:38.05 Gate.setIR 1750,5000,0,6 @11:19:38.17 Gate.setIR 0,5000,0,6 @11:19:38.22 Loaded Lyse-n-Go Cartridge @11:19:38.23 PuckHeater.configure HeatConfig @11:19:38.29 Gate.power :heat,:ON,limit to 5000mA @11:19:58.17 -> Cmd.startFiltering @11:19:58.40 Duration of filtering limited to 1:05:00 @11:19:58.65 Priming sample loop w/100ml, bypass w/10ml @11:19:58.72 SP.setPosition! 0ml @11:19:58.76 Intake.open @11:19:58.81 Exhaust.open @11:19:59.57 Sea @-42.9psia @11:19:59.58 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @11:20:05.35 Vacuum tare is 23% of full scale @11:20:05.37 PB.open @11:20:05.40 Exhaust.close @11:20:06.22 Sea @-42.9psia @11:20:06.31 SP.reconfigure SPprime @11:20:06.37 SP.seek 10ml,1:21 @11:20:17.25 SP.setPosition! 0ml @11:20:17.29 PB.close @11:20:17.32 Exhaust.open @11:20:18.11 Sea @-42.9psia @11:20:18.19 SP.reconfigure SPprime @11:20:18.26 SP.seek 100ml,8:51 @11:22:12.40 Pressurizing cartridge to 26psi @11:22:12.41 Intake.close @11:22:13.11 SP.setPosition! 0ml @11:22:13.15 PB.open @11:22:13.89 TV.seek :crack @11:22:14.68 TV.seek :clear @11:22:15.48 Intake.open @11:22:16.92 PB.close @11:22:17.64 Exhaust.close @11:22:18.41 Sea @-42.9psia @11:22:18.49 SP.reconfigure SPcharge @11:22:18.55 SP.seek 7.5ml,1:08 @11:22:28.97 Cartridge pressurized with 0.506ml to 26.3psi @11:22:29.20 delay 3 seconds @11:22:32.27 Cartridge at 22.0psi 3 seconds later @11:22:32.35 TV.seek :cartridge @11:22:34.17 TV.seek :clear @11:22:35.99 Sampling 1000.0ml @11:22:36.00 SP.setPosition! 0ml @11:22:36.05 Exhaust.open @11:22:36.82 Sea @-42.9psia @11:22:36.91 SP.reconfigure SPsample @11:22:36.97 SP.seek 1000ml,1:23:53 @11:22:37.93 Cartridge 46 sampling 0/1000ml at 0.17ml/s with 4.6psi vacuum @11:23:37.94 Cartridge 46 sampling 32/1000ml at 0.55ml/s with 22.5psi vacuum @11:24:37.93 Cartridge 46 sampling 63/1000ml at 0.54ml/s with 24.0psi vacuum @11:25:37.94 Cartridge 46 sampling 95/1000ml at 0.50ml/s with 26.0psi vacuum @11:26:37.94 Cartridge 46 sampling 126/1000ml at 0.49ml/s with 23.5psi vacuum @11:27:37.94 Cartridge 46 sampling 156/1000ml at 0.48ml/s with 21.6psi vacuum @11:28:37.94 Cartridge 46 sampling 187/1000ml at 0.48ml/s with 28.0psi vacuum @11:29:37.93 Cartridge 46 sampling 216/1000ml at 0.53ml/s with 24.0psi vacuum @11:30:37.94 Cartridge 46 sampling 246/1000ml at 0.46ml/s with 22.6psi vacuum @11:31:37.94 Cartridge 46 sampling 275/1000ml at 0.51ml/s with 21.2psi vacuum @11:32:37.94 Cartridge 46 sampling 304/1000ml at 0.49ml/s with 24.1psi vacuum @11:33:37.94 Cartridge 46 sampling 332/1000ml at 0.50ml/s with 22.9psi vacuum @11:34:37.93 Cartridge 46 sampling 360/1000ml at 0.43ml/s with 21.9psi vacuum @11:35:37.94 Cartridge 46 sampling 388/1000ml at 0.43ml/s with 20.6psi vacuum @11:36:37.94 Cartridge 46 sampling 415/1000ml at 0.44ml/s with 25.5psi vacuum @11:37:37.94 Cartridge 46 sampling 441/1000ml at 0.43ml/s with 17.5psi vacuum @11:38:37.94 Cartridge 46 sampling 467/1000ml at 0.42ml/s with 17.5psi vacuum @11:39:38.19 Cartridge 46 sampling 493/1000ml at 0.40ml/s with 22.5psi vacuum @11:40:37.94 Cartridge 46 sampling 518/1000ml at 0.43ml/s with 25.9psi vacuum @11:41:37.95 Cartridge 46 sampling 543/1000ml at 0.38ml/s with 21.0psi vacuum @11:42:37.95 Cartridge 46 sampling 567/1000ml at 0.37ml/s with 26.8psi vacuum @11:43:37.94 Cartridge 46 sampling 591/1000ml at 0.36ml/s with 25.3psi vacuum @11:44:37.93 Cartridge 46 sampling 614/1000ml at 0.37ml/s with 19.1psi vacuum @11:45:37.94 Cartridge 46 sampling 637/1000ml at 0.35ml/s with 26.5psi vacuum @11:46:37.94 Cartridge 46 sampling 659/1000ml at 0.33ml/s with 25.4psi vacuum @11:47:37.94 Cartridge 46 sampling 680/1000ml at 0.39ml/s with 22.7psi vacuum @11:48:37.94 Cartridge 46 sampling 701/1000ml at 0.37ml/s with 25.9psi vacuum @11:49:37.94 Cartridge 46 sampling 722/1000ml at 0.36ml/s with 20.3psi vacuum @11:50:37.94 Cartridge 46 sampling 742/1000ml at 0.34ml/s with 19.3psi vacuum @11:51:37.94 Cartridge 46 sampling 761/1000ml at 0.33ml/s with 19.3psi vacuum @11:52:37.94 Cartridge 46 sampling 780/1000ml at 0.34ml/s with 25.9psi vacuum @11:53:37.94 Cartridge 46 sampling 799/1000ml at 0.35ml/s with 24.1psi vacuum @11:54:37.94 Cartridge 46 sampling 817/1000ml at 0.27ml/s with 25.2psi vacuum @11:55:37.94 Cartridge 46 sampling 834/1000ml at 0.25ml/s with 23.8psi vacuum @11:56:37.94 Cartridge 46 sampling 852/1000ml at 0.27ml/s with 24.3psi vacuum @11:57:37.94 Cartridge 46 sampling 868/1000ml at 0.31ml/s with 20.6psi vacuum @11:58:37.94 Cartridge 46 sampling 885/1000ml at 0.26ml/s with 25.4psi vacuum @11:59:37.93 Cartridge 46 sampling 901/1000ml at 0.24ml/s with 23.9psi vacuum @12:00:37.94 Cartridge 46 sampling 916/1000ml at 0.24ml/s with 21.9psi vacuum @12:01:37.94 Cartridge 46 sampling 932/1000ml at 0.23ml/s with 24.0psi vacuum @12:02:37.94 Cartridge 46 sampling 947/1000ml at 0.24ml/s with 25.7psi vacuum @12:03:37.94 Cartridge 46 sampling 961/1000ml at 0.27ml/s with 24.2psi vacuum @12:04:37.94 Cartridge 46 sampling 976/1000ml at 0.26ml/s with 24.9psi vacuum @12:05:37.94 Cartridge 46 sampling 990/1000ml at 0.21ml/s with 23.3psi vacuum @12:06:22.19 Intake.close @12:06:22.22 Exhaust.close @12:06:23.02 Sampled 1000.0ml @12:06:23.10 TV.seek :bypass @12:06:24.76 TV.seek :clear @12:06:27.05 -> Cmd.startProcessing @12:06:28.84 SPR.command \"read_sequences\" @12:06:29.06 SPR.command \"stats_on\" @12:06:29.10 SPR.command \"start 2 0\" @12:06:31.10 Begun log /var/log/esp/spr18aug0412h.spr @12:06:31.91 Handoff.select :waste @12:06:32.03 ArS.seek empty @12:06:49.77 PinPort.seek :cartridge @12:06:55.49 LyS1.seek 1350ul @12:07:01.69 PV.seek :reagent @12:07:02.54 AirS.seek :tVent @12:07:04.61 delay 2 seconds @12:07:06.64 AirS.reconfigure EvacAirS @12:07:06.69 AirS.seek :evac @12:07:29.02 delay 15 seconds @12:07:44.04 AirS.reconfigure AirSconfig @12:07:44.09 AirS.seek :tVent @12:07:58.32 delay 2 seconds @12:08:00.34 AirS.reconfigure EvacAirS @12:08:00.40 AirS.seek :evac @12:08:22.72 delay 15 seconds @12:08:37.75 AirS.reconfigure AirSconfig @12:08:37.80 AirS.seek :tVent @12:08:52.03 delay 2 seconds @12:08:54.06 AirS.reconfigure EvacAirS @12:08:54.12 AirS.seek :evac @12:09:16.44 delay 15 seconds @12:09:31.47 AirS.reconfigure AirSconfig @12:09:31.52 AirS.seek :tVent @12:09:45.74 delay 2 seconds @12:09:47.77 AirS.reconfigure EvacAirS @12:09:47.82 AirS.seek :evac @12:10:10.13 delay 15 seconds @12:10:25.16 AirS.reconfigure AirSconfig @12:10:25.21 AirS.seek :tVent @12:10:39.44 delay 2 seconds @12:10:41.47 AirS.reconfigure EvacAirS @12:10:41.52 AirS.seek :evac @12:11:03.84 delay 15 seconds @12:11:18.87 delay 1:30 @12:12:48.90 AirS.reconfigure AirSconfig @12:12:49.00 AirS.seek :fVent @12:12:50.44 delay 15 seconds @12:13:05.46 LyS1.reconfigure SlowLyS1 @12:13:05.56 LyS1.seek 1050ul @12:13:35.60 delay 5 seconds @12:13:40.62 Handoff.select :blocked @12:13:40.78 LyS1.seek 450ul @12:14:40.79 delay 10 seconds @12:14:50.82 AirS.reconfigure SlowAirS @12:14:50.93 AirS.reconfigure to mix into 1.37806 parts LyS1 @12:14:51.28 AirS.seek evac @12:14:51.32 LyS1.seek 300ul @12:15:06.43 AirS.reconfigure AirSconfig @12:15:06.49 AirS.seek :full @12:15:20.70 LyS1.seek :empty @12:15:50.81 PuckHeater.seek 86.1C @12:17:14.26 PuckHeater.hold 15:00,86.1C @12:32:13.09 LyS1.reconfigure LyS1config @12:32:13.18 LyS1.seek 900ul @12:32:21.51 AirS.seek :tVent @12:32:21.60 delay 2 seconds @12:32:23.63 AirS.reconfigure HalfSpeedAirS @12:32:23.68 AirS.seek :evac @12:32:51.42 delay 15 seconds @12:33:06.45 AirS.reconfigure AirSconfig @12:33:06.50 AirS.seek :tVent @12:33:20.71 delay 2 seconds @12:33:22.74 AirS.reconfigure HalfSpeedAirS @12:33:22.79 AirS.seek :evac @12:33:50.53 delay 15 seconds @12:34:05.57 AirS.reconfigure AirSconfig @12:34:05.62 AirS.seek :tVent @12:34:19.86 delay 2 seconds @12:34:21.89 AirS.reconfigure HalfSpeedAirS @12:34:21.94 AirS.seek :evac @12:34:49.66 delay 15 seconds @12:35:04.68 delay 1:00 @12:36:04.71 Handoff.select :waste @12:36:04.82 delay 15 seconds @12:36:19.86 AirS.seek :fVent @12:36:22.05 delay 10 seconds @12:36:32.08 AirS.reconfigure AirSconfig @12:36:32.13 AirS.seek :evac @12:36:33.57 Lysis Recovered in LyS1 @12:36:33.58 Lysis Complete @12:36:33.59 Handoff.select :waste @12:36:33.62 LyS2.seek :full @12:36:33.70 LyS1.reconfigure DAmixLyS1 @12:36:33.76 LyS2.reconfigure DAmixLyS2 @12:36:34.18 LyS2.seek 1480ul @12:36:34.22 LyS1.seek 650ul @12:37:21.53 delay 5 seconds @12:37:26.55 Handoff.select :SPR @12:37:26.66 SPR.command \"start 1 14\" @12:37:27.62 Running Experiment-Rep #1 @12:37:27.63 delay 10 seconds @12:37:37.65 SPR.command \"start 1 0\" @12:37:40.99 Waiting up to 4:00 for SPR to enter state 1 @12:39:45.23 SPR.command \"hold_on\" @12:39:46.98 LyS2.seek 1130ul @12:39:47.01 LyS1.seek 300ul @12:40:53.20 delay 15 seconds @12:41:08.22 SPR.command \"hold_off\" @12:41:11.44 Waiting up to 25:00 for SPR to enter state 7 @13:01:16.28 Handoff.select :waste @13:01:16.40 LyS1.reconfigure LyS1config @13:01:16.47 LyS2.reconfigure LyS2config @13:01:16.53 Rep #1 SPR Analysis has Completed @13:01:17.22 Ended log /var/log/esp/spr18aug0412h.spr @13:01:17.24 SPR.command \"stats_off\" @13:01:19.47 -> Cmd.stop @13:01:19.56 Handoff.select :close @13:01:19.73 Gate.power :heat,:OFF @13:01:19.75 Gate.power :cartridge,:OFF @13:01:19.78 PV.seek :locked @13:01:20.68 PinPort.seek :clear @13:01:26.45 ArS.seek :clear @13:01:26.57 LyS1.seek :clear @13:01:26.71 LyS2.seek :clear @13:01:26.90 AirS.seek :clear @13:01:44.21 ArS.coast @13:01:44.26 LyS1.coast @13:01:44.31 LyS2.coast @13:01:44.35 AirS.coast @13:01:44.41 TV.coast @13:01:44.46 SP.coast @13:01:44.51 SC.coast @13:01:44.56 PV.coast @13:01:44.61 PinPort.coast @13:01:44.79 Gate.power :motor,:OFF @13:01:44.81 Gate.power :main,:OFF @13:01:44.83 Gate.power :core,:OFF @13:01:44.85 Gate.power :analytic1,:OFF @13:01:44.87 Gate.power :analytic2,:OFF @13:01:44.89 Gate.power :analytic3,:OFF @13:01:44.91 Safely stopped and ready to power off ##-- file closed 2018-08-04T20:17:22.421Z ##-- file opened 2018-08-04T20:17:22.432Z @13:16:53.43 -> showStatus @13:16:55.44 -> Cmd.loadCartridge @13:16:55.56 Gate.power :main,:ON,limit to 5000mA @13:16:55.58 Gate.power :analytic1,:ON,limit to 5000mA @13:16:55.60 Gate.power :analytic2,:ON,limit to 5000mA @13:16:55.62 Gate.power :analytic3,:ON,limit to 5000mA @13:16:55.77 Powering core bus @13:16:58.08 SPR Module 1, FW Rev. B, FPGA Rev. C @13:16:58.17 ArS.configure ArSconfig @13:16:58.28 LyS1.configure LyS1config @13:16:58.39 LyS2.configure LyS2config @13:16:58.50 AirS.configure AirSconfig @13:16:58.62 TV.configure TVconfig @13:16:58.74 SP.configure SPconfig @13:16:58.85 SC.configure SCconfig @13:16:58.98 PV.configure PVconfig @13:16:59.09 PinPort.configure PPconfig @13:16:59.21 Sea.configure SPconfig @13:16:59.27 All elf controllers running firmware version 4.46 @13:16:59.28 Gate.power :motor,:ON,limit to 5000mA @13:16:59.77 TV.reconfigure TVconfig @13:16:59.82 AirS.reconfigure AirSconfig @13:16:59.85 LyS1.reconfigure LyS1config @13:16:59.90 PinPort.reconfigure PPconfig @13:16:59.93 PV.reconfigure PVconfig @13:16:59.97 ArS.reconfigure ArSconfig @13:17:00.01 LyS2.reconfigure LyS2config @13:17:00.24 AirS.home.jog 0 @13:17:00.27 TV.home.jog 0 @13:17:00.30 PinPort.home.jog 0 @13:17:00.32 PV.home.jog 0 @13:17:00.36 ArS.home.jog 0 @13:17:00.38 LyS1.home.jog 0 @13:17:00.41 LyS2.home.jog 0 @13:17:00.99 PinPort.reconfigure HomePP @13:17:01.03 PV.reconfigure HomePV @13:17:01.07 TV.reconfigure HomeTV @13:17:01.10 AirS.reconfigure HomeMagSyringe @13:17:01.27 PinPort.jog -109 @13:17:01.30 TV.jog -554 @13:17:01.38 PV.jog -1508 @13:17:01.41 AirS.jog -340 @13:17:01.60 LyS1.reconfigure HomeSyringe @13:17:01.62 LyS2.reconfigure HomeSyringe @13:17:01.67 ArS.reconfigure HomeMagSyringe @13:17:01.79 LyS2.jog -277 @13:17:01.82 LyS1.jog -282 @13:17:01.94 ArS.jog -288 @13:17:02.06 PinPort.setRawPosition! 0 @13:17:02.11 PinPort.seek :home @13:17:02.21 PinPort.reconfigure PPconfig @13:17:02.23 AirS.setRawPosition! -50 @13:17:02.30 AirS.seek :home @13:17:02.68 LyS2.setRawPosition! -50 @13:17:02.70 ArS.setRawPosition! -50 @13:17:02.73 PV.setRawPosition! -50 @13:17:02.77 LyS1.setRawPosition! -50 @13:17:02.83 LyS2.seek :home @13:17:02.92 PV.seek :home @13:17:03.00 ArS.seek :home @13:17:03.04 LyS1.seek :home @13:17:03.26 AirS.reconfigure AirSconfig @13:17:03.39 TV.setRawPosition! 0 @13:17:03.41 LyS2.reconfigure LyS2config @13:17:03.51 TV.seek :home @13:17:03.65 TV.reconfigure TVconfig @13:17:03.68 LyS1.reconfigure LyS1config @13:17:03.72 PV.reconfigure PVconfig @13:17:03.75 ArS.reconfigure ArSconfig @13:17:04.37 TV.seek :clear @13:17:05.98 TV.coast @13:17:06.03 SC.reconfigure HomeSC @13:17:06.10 SC.home.jog 0 @13:17:06.86 SC.reconfigure SCconfig @13:17:06.91 SC.seek 46 @13:17:07.01 Selecting Cartridge 45 @13:17:07.31 SC.seek 45 @13:17:07.81 LyS2.seek :locked @13:17:07.94 PV.seek :locked @13:17:08.07 AirS.seek :locked @13:17:12.16 Powering cartridge bus @13:17:14.28 Gate.setIR 1750,5000,0,6 @13:17:14.41 Gate.setIR 0,5000,0,6 @13:17:14.44 Loaded Archive Cartridge @13:17:14.45 Gate.power :cartridge,:OFF @13:17:33.80 -> Cmd.startFiltering @13:17:34.03 Duration of filtering limited to 1:05:00 @13:17:34.29 Priming sample loop w/100ml, bypass w/10ml @13:17:34.36 SP.setPosition! 0ml @13:17:34.40 Intake.open @13:17:34.43 Exhaust.open @13:17:35.26 Sea @-42.9psia @13:17:35.27 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @13:17:40.80 Vacuum tare is 22% of full scale @13:17:40.82 PB.open @13:17:40.85 Exhaust.close @13:17:41.92 Sea @-42.9psia @13:17:42.00 SP.reconfigure SPprime @13:17:42.07 SP.seek 10ml,1:21 @13:17:52.95 SP.setPosition! 0ml @13:17:52.99 PB.close @13:17:53.02 Exhaust.open @13:17:53.85 Sea @-42.9psia @13:17:53.93 SP.reconfigure SPprime @13:17:53.99 SP.seek 100ml,8:51 @13:19:48.36 Pressurizing cartridge to 26psi @13:19:48.37 Intake.close @13:19:49.14 SP.setPosition! 0ml @13:19:49.19 PB.open @13:19:49.98 TV.seek :crack @13:19:50.77 TV.seek :clear @13:19:51.56 Intake.open @13:19:53.03 PB.close @13:19:53.77 Exhaust.close @13:19:54.56 Sea @-42.9psia @13:19:54.64 SP.reconfigure SPcharge @13:19:54.70 SP.seek 7.5ml,1:08 @13:19:59.72 Cartridge pressurized with 0.114ml to 26.4psi @13:19:59.94 delay 3 seconds @13:20:03.01 Cartridge at 20.2psi 3 seconds later @13:20:03.09 TV.seek :cartridge @13:20:04.92 TV.seek :clear @13:20:06.76 Sampling 1000.0ml @13:20:06.78 SP.setPosition! 0ml @13:20:06.85 Exhaust.open @13:20:07.64 Sea @-42.9psia @13:20:07.73 SP.reconfigure SPsample @13:20:07.80 SP.seek 1000ml,1:23:53 @13:20:08.75 Cartridge 45 sampling 0/1000ml at 0.17ml/s with 7.1psi vacuum @13:21:08.76 Cartridge 45 sampling 21/1000ml at 0.37ml/s with 20.8psi vacuum @13:22:08.76 Cartridge 45 sampling 41/1000ml at 0.37ml/s with 24.8psi vacuum @13:23:08.76 Cartridge 45 sampling 61/1000ml at 0.37ml/s with 22.0psi vacuum @13:24:08.76 Cartridge 45 sampling 82/1000ml at 0.34ml/s with 24.2psi vacuum @13:25:08.76 Cartridge 45 sampling 102/1000ml at 0.36ml/s with 23.9psi vacuum @13:26:08.76 Cartridge 45 sampling 122/1000ml at 0.32ml/s with 23.7psi vacuum @13:27:08.76 Cartridge 45 sampling 141/1000ml at 0.36ml/s with 22.9psi vacuum @13:28:08.76 Cartridge 45 sampling 161/1000ml at 0.29ml/s with 19.6psi vacuum @13:29:08.76 Cartridge 45 sampling 180/1000ml at 0.33ml/s with 17.8psi vacuum @13:30:08.76 Cartridge 45 sampling 200/1000ml at 0.30ml/s with 20.9psi vacuum @13:31:08.76 Cartridge 45 sampling 219/1000ml at 0.32ml/s with 24.6psi vacuum @13:32:08.76 Cartridge 45 sampling 238/1000ml at 0.30ml/s with 23.2psi vacuum @13:33:08.76 Cartridge 45 sampling 258/1000ml at 0.29ml/s with 26.1psi vacuum @13:34:08.76 Cartridge 45 sampling 277/1000ml at 0.30ml/s with 25.5psi vacuum @13:35:08.76 Cartridge 45 sampling 296/1000ml at 0.31ml/s with 25.0psi vacuum @13:36:08.76 Cartridge 45 sampling 315/1000ml at 0.35ml/s with 25.2psi vacuum @13:37:09.02 Cartridge 45 sampling 334/1000ml at 0.32ml/s with 16.6psi vacuum @13:38:08.76 Cartridge 45 sampling 353/1000ml at 0.31ml/s with 24.6psi vacuum @13:39:08.76 Cartridge 45 sampling 372/1000ml at 0.29ml/s with 19.2psi vacuum @13:40:08.76 Cartridge 45 sampling 391/1000ml at 0.34ml/s with 19.8psi vacuum @13:41:08.76 Cartridge 45 sampling 410/1000ml at 0.34ml/s with 24.5psi vacuum @13:42:08.76 Cartridge 45 sampling 429/1000ml at 0.31ml/s with 24.4psi vacuum @13:43:08.76 Cartridge 45 sampling 447/1000ml at 0.31ml/s with 24.0psi vacuum @13:44:08.76 Cartridge 45 sampling 466/1000ml at 0.31ml/s with 23.5psi vacuum @13:45:08.76 Cartridge 45 sampling 485/1000ml at 0.34ml/s with 22.5psi vacuum @13:46:08.76 Cartridge 45 sampling 504/1000ml at 0.33ml/s with 19.5psi vacuum @13:47:08.76 Cartridge 45 sampling 522/1000ml at 0.28ml/s with 26.5psi vacuum @13:48:08.76 Cartridge 45 sampling 541/1000ml at 0.34ml/s with 23.5psi vacuum @13:49:08.76 Cartridge 45 sampling 560/1000ml at 0.29ml/s with 20.0psi vacuum @13:50:08.76 Cartridge 45 sampling 579/1000ml at 0.32ml/s with 26.6psi vacuum @13:51:08.76 Cartridge 45 sampling 597/1000ml at 0.29ml/s with 21.7psi vacuum @13:52:08.76 Cartridge 45 sampling 616/1000ml at 0.34ml/s with 25.2psi vacuum @13:53:08.76 Cartridge 45 sampling 634/1000ml at 0.30ml/s with 24.3psi vacuum @13:54:08.76 Cartridge 45 sampling 653/1000ml at 0.29ml/s with 25.6psi vacuum @13:55:08.76 Cartridge 45 sampling 672/1000ml at 0.28ml/s with 18.6psi vacuum @13:56:08.76 Cartridge 45 sampling 690/1000ml at 0.32ml/s with 19.3psi vacuum @13:57:08.76 Cartridge 45 sampling 709/1000ml at 0.34ml/s with 20.2psi vacuum @13:58:08.76 Cartridge 45 sampling 727/1000ml at 0.34ml/s with 21.3psi vacuum @13:59:08.76 Cartridge 45 sampling 746/1000ml at 0.34ml/s with 20.7psi vacuum @14:00:08.76 Cartridge 45 sampling 764/1000ml at 0.33ml/s with 20.3psi vacuum @14:01:08.76 Cartridge 45 sampling 783/1000ml at 0.30ml/s with 20.9psi vacuum @14:02:08.76 Cartridge 45 sampling 801/1000ml at 0.27ml/s with 26.4psi vacuum @14:03:08.76 Cartridge 45 sampling 820/1000ml at 0.30ml/s with 24.9psi vacuum @14:04:08.76 Cartridge 45 sampling 838/1000ml at 0.31ml/s with 16.9psi vacuum @14:05:08.76 Cartridge 45 sampling 856/1000ml at 0.30ml/s with 22.9psi vacuum @14:06:08.76 Cartridge 45 sampling 875/1000ml at 0.28ml/s with 19.9psi vacuum @14:07:08.76 Cartridge 45 sampling 893/1000ml at 0.35ml/s with 23.9psi vacuum @14:08:08.76 Cartridge 45 sampling 911/1000ml at 0.30ml/s with 23.9psi vacuum @14:09:08.76 Cartridge 45 sampling 929/1000ml at 0.26ml/s with 24.9psi vacuum @14:10:08.76 Cartridge 45 sampling 948/1000ml at 0.31ml/s with 16.5psi vacuum @14:11:08.76 Cartridge 45 sampling 966/1000ml at 0.33ml/s with 22.2psi vacuum @14:12:08.76 Cartridge 45 sampling 984/1000ml at 0.30ml/s with 24.0psi vacuum @14:13:03.07 Intake.close @14:13:03.12 Exhaust.close @14:13:04.13 Sampled 1000.0ml @14:13:04.22 TV.seek :bypass @14:13:05.84 TV.seek :clear @14:13:08.22 -> Cmd.startProcessing @14:13:08.47 PV.seek :reagent @14:13:09.31 Sample loop closed. Ready to Deliver Reagents @14:13:09.33 ArS.seek :sealed @14:13:13.61 ArS.seek :evac @14:13:27.54 delay 20 seconds @14:13:47.58 ArS.seek :clear @14:14:05.30 RNAlater soak for 10:00 @14:24:05.33 ArS.seek :tVent @14:24:07.90 delay 1 second @14:24:08.93 ArS.seek :evac @14:24:24.55 delay 10 seconds @14:24:34.59 Sample preserved and evacuated @14:24:35.82 -> Cmd.stop @14:24:35.96 PV.seek :locked @14:24:36.85 PinPort.seek :clear @14:24:36.99 ArS.seek :clear @14:24:37.17 LyS2.seek :clear @14:24:37.29 AirS.seek :clear @14:24:54.75 ArS.coast @14:24:54.80 LyS1.coast @14:24:54.85 LyS2.coast @14:24:54.90 AirS.coast @14:24:54.95 TV.coast @14:24:55.00 SP.coast @14:24:55.05 SC.coast @14:24:55.10 PV.coast @14:24:55.15 PinPort.coast @14:24:55.34 Gate.power :motor,:OFF @14:24:55.35 Gate.power :main,:OFF @14:24:55.38 Gate.power :core,:OFF @14:24:55.40 Gate.power :analytic1,:OFF @14:24:55.42 Gate.power :analytic2,:OFF @14:24:55.44 Gate.power :analytic3,:OFF @14:24:55.46 Safely stopped and ready to power off ##-- file closed 2018-08-04T21:29:07.828Z ##-- file opened 2018-08-04T21:29:07.830Z @14:28:32.20 -> showStatus @14:28:34.21 -> Cmd.loadCartridge @14:28:34.33 Gate.power :main,:ON,limit to 5000mA @14:28:34.34 Gate.power :analytic1,:ON,limit to 5000mA @14:28:34.36 Gate.power :analytic2,:ON,limit to 5000mA @14:28:34.39 Gate.power :analytic3,:ON,limit to 5000mA @14:28:34.53 Powering core bus @14:28:36.82 SPR Module 1, FW Rev. B, FPGA Rev. C @14:28:36.92 ArS.configure ArSconfig @14:28:37.02 LyS1.configure LyS1config @14:28:37.14 LyS2.configure LyS2config @14:28:37.24 AirS.configure AirSconfig @14:28:37.35 TV.configure TVconfig @14:28:37.47 SP.configure SPconfig @14:28:37.59 SC.configure SCconfig @14:28:37.71 PV.configure PVconfig @14:28:37.83 PinPort.configure PPconfig @14:28:37.95 Sea.configure SPconfig @14:28:38.01 All elf controllers running firmware version 4.46 @14:28:38.03 Gate.power :motor,:ON,limit to 5000mA @14:28:38.18 ArS.reconfigure ArSconfig @14:28:38.48 ArS.home.jog 0 @14:28:38.61 PinPort.reconfigure PPconfig @14:28:38.68 PV.reconfigure PVconfig @14:28:38.72 AirS.reconfigure AirSconfig @14:28:38.75 LyS1.reconfigure LyS1config @14:28:38.78 TV.reconfigure TVconfig @14:28:38.86 LyS2.reconfigure LyS2config @14:28:39.01 PinPort.home.jog 0 @14:28:39.06 PV.home.jog 0 @14:28:39.10 LyS1.home.jog 0 @14:28:39.13 TV.home.jog 0 @14:28:39.15 LyS2.home.jog 0 @14:28:39.19 AirS.home.jog 0 @14:28:39.54 ArS.reconfigure HomeMagSyringe @14:28:39.74 PV.reconfigure HomePV @14:28:39.78 TV.reconfigure HomeTV @14:28:39.82 LyS1.reconfigure HomeSyringe @14:28:39.92 PinPort.reconfigure HomePP @14:28:40.00 ArS.jog -285 @14:28:40.16 PV.jog -1502 @14:28:40.18 TV.jog -553 @14:28:40.22 PinPort.jog -107 @14:28:40.26 LyS1.jog -282 @14:28:40.47 LyS2.reconfigure HomeSyringe @14:28:40.48 AirS.reconfigure HomeMagSyringe @14:28:40.57 LyS2.jog -272 @14:28:40.60 AirS.jog -338 @14:28:40.73 PinPort.setRawPosition! 0 @14:28:40.75 ArS.setRawPosition! -50 @14:28:40.82 PinPort.seek :home @14:28:40.86 ArS.seek :home @14:28:40.99 PinPort.reconfigure PPconfig @14:28:41.25 LyS1.setRawPosition! -50 @14:28:41.36 LyS1.seek :home @14:28:41.46 LyS2.setRawPosition! -50 @14:28:41.50 AirS.setRawPosition! -50 @14:28:41.54 ArS.reconfigure ArSconfig @14:28:41.63 LyS2.seek :home @14:28:41.71 AirS.seek :home @14:28:41.86 PV.setRawPosition! -50 @14:28:41.88 LyS1.reconfigure LyS1config @14:28:41.95 PV.seek :home @14:28:42.10 AirS.reconfigure AirSconfig @14:28:42.12 LyS2.reconfigure LyS2config @14:28:42.23 TV.setRawPosition! 0 @14:28:42.30 TV.seek :home @14:28:42.40 TV.reconfigure TVconfig @14:28:42.43 PV.reconfigure PVconfig @14:28:42.71 TV.seek :clear @14:28:44.34 TV.coast @14:28:44.39 SC.reconfigure HomeSC @14:28:44.45 SC.home.jog 0 @14:28:45.22 SC.reconfigure SCconfig @14:28:45.28 SC.seek 45 @14:28:45.38 Selecting Cartridge 44 @14:28:45.68 SC.seek 44 @14:28:46.18 LyS2.seek :locked @14:28:46.31 PV.seek :locked @14:28:46.45 AirS.seek :locked @14:28:50.52 Powering cartridge bus @14:28:52.64 Gate.setIR 1750,5000,0,6 @14:28:52.77 Gate.setIR 0,5000,0,6 @14:28:52.83 Loaded Lyse-n-Go Cartridge @14:28:52.84 PuckHeater.configure HeatConfig @14:28:52.89 Gate.power :heat,:ON,limit to 5000mA @14:29:13.01 -> Cmd.startFiltering @14:29:13.24 Duration of filtering limited to 1:05:00 @14:29:13.49 Priming sample loop w/100ml, bypass w/10ml @14:29:13.57 SP.setPosition! 0ml @14:29:13.61 Intake.open @14:29:13.63 Exhaust.open @14:29:14.47 Sea @-42.9psia @14:29:14.48 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @14:29:20.00 Vacuum tare is 23% of full scale @14:29:20.02 PB.open @14:29:20.30 Exhaust.close @14:29:21.10 Sea @-42.9psia @14:29:21.18 SP.reconfigure SPprime @14:29:21.25 SP.seek 10ml,1:21 @14:29:32.13 SP.setPosition! 0ml @14:29:32.17 PB.close @14:29:32.20 Exhaust.open @14:29:32.99 Sea @-42.9psia @14:29:33.08 SP.reconfigure SPprime @14:29:33.15 SP.seek 100ml,8:51 @14:31:28.04 Pressurizing cartridge to 26psi @14:31:28.05 Intake.close @14:31:28.81 SP.setPosition! 0ml @14:31:28.85 PB.open @14:31:29.63 TV.seek :crack @14:31:30.42 TV.seek :clear @14:31:31.22 Intake.open @14:31:32.67 PB.close @14:31:33.40 Exhaust.close @14:31:34.15 Sea @-42.9psia @14:31:34.23 SP.reconfigure SPcharge @14:31:34.30 SP.seek 7.5ml,1:08 @14:31:39.20 Cartridge pressurized with 0.109ml to 26.1psi @14:31:39.44 delay 3 seconds @14:31:42.51 Cartridge at 22.3psi 3 seconds later @14:31:42.59 TV.seek :cartridge @14:31:44.40 TV.seek :clear @14:31:46.21 Sampling 1000.0ml @14:31:46.22 SP.setPosition! 0ml @14:31:46.27 Exhaust.open @14:31:47.03 Sea @-42.9psia @14:31:47.12 SP.reconfigure SPsample @14:31:47.18 SP.seek 1000ml,1:23:53 @14:31:48.14 Cartridge 44 sampling 0/1000ml at 0.17ml/s with 3.7psi vacuum @14:32:48.15 Cartridge 44 sampling 31/1000ml at 0.55ml/s with 25.5psi vacuum @14:33:48.15 Cartridge 44 sampling 63/1000ml at 0.49ml/s with 22.7psi vacuum @14:34:48.15 Cartridge 44 sampling 94/1000ml at 0.51ml/s with 18.1psi vacuum @14:35:48.15 Cartridge 44 sampling 125/1000ml at 0.50ml/s with 21.7psi vacuum @14:36:48.15 Cartridge 44 sampling 156/1000ml at 0.52ml/s with 26.0psi vacuum @14:37:48.15 Cartridge 44 sampling 187/1000ml at 0.52ml/s with 17.4psi vacuum -39.0m deep (-42.9psia) @14:38:48.15 Cartridge 44 sampling 219/1000ml at 0.51ml/s with 25.7psi vacuum @14:39:48.15 Cartridge 44 sampling 250/1000ml at 0.52ml/s with 17.6psi vacuum @14:40:48.15 Cartridge 44 sampling 281/1000ml at 0.53ml/s with 25.1psi vacuum @14:41:48.15 Cartridge 44 sampling 312/1000ml at 0.49ml/s with 21.6psi vacuum @14:42:48.15 Cartridge 44 sampling 343/1000ml at 0.51ml/s with 17.4psi vacuum @14:43:48.15 Cartridge 44 sampling 374/1000ml at 0.54ml/s with 23.0psi vacuum @14:44:48.15 Cartridge 44 sampling 405/1000ml at 0.54ml/s with 25.8psi vacuum @14:45:48.15 Cartridge 44 sampling 436/1000ml at 0.53ml/s with 25.4psi vacuum @14:46:48.15 Cartridge 44 sampling 467/1000ml at 0.53ml/s with 24.9psi vacuum @14:47:48.15 Cartridge 44 sampling 498/1000ml at 0.54ml/s with 25.7psi vacuum @14:48:48.41 Cartridge 44 sampling 529/1000ml at 0.49ml/s with 21.2psi vacuum @14:49:48.15 Cartridge 44 sampling 560/1000ml at 0.52ml/s with 19.1psi vacuum @14:50:48.15 Cartridge 44 sampling 590/1000ml at 0.48ml/s with 20.4psi vacuum @14:51:48.15 Cartridge 44 sampling 621/1000ml at 0.53ml/s with 25.1psi vacuum @14:52:48.15 Cartridge 44 sampling 652/1000ml at 0.51ml/s with 17.7psi vacuum @14:53:48.15 Cartridge 44 sampling 683/1000ml at 0.51ml/s with 25.2psi vacuum @14:54:48.15 Cartridge 44 sampling 714/1000ml at 0.53ml/s with 20.6psi vacuum @14:55:48.15 Cartridge 44 sampling 745/1000ml at 0.49ml/s with 20.9psi vacuum @14:56:48.15 Cartridge 44 sampling 775/1000ml at 0.54ml/s with 25.5psi vacuum @14:57:48.15 Cartridge 44 sampling 806/1000ml at 0.50ml/s with 17.9psi vacuum @14:58:48.15 Cartridge 44 sampling 837/1000ml at 0.54ml/s with 23.2psi vacuum @14:59:48.15 Cartridge 44 sampling 868/1000ml at 0.49ml/s with 20.5psi vacuum @15:00:48.15 Cartridge 44 sampling 899/1000ml at 0.55ml/s with 22.5psi vacuum @15:01:48.15 Cartridge 44 sampling 929/1000ml at 0.49ml/s with 20.5psi vacuum @15:02:48.15 Cartridge 44 sampling 960/1000ml at 0.54ml/s with 24.8psi vacuum @15:03:48.15 Cartridge 44 sampling 991/1000ml at 0.50ml/s with 18.0psi vacuum @15:04:10.07 Intake.close @15:04:10.12 Exhaust.close @15:04:10.85 Sampled 1000.0ml @15:04:10.94 TV.seek :bypass @15:04:12.55 TV.seek :clear @15:04:14.71 -> Cmd.startProcessing @15:04:16.49 SPR.command \"read_sequences\" @15:04:17.14 SPR.command \"stats_on\" @15:04:17.18 SPR.command \"start 2 0\" @15:04:19.18 Begun log /var/log/esp/spr18aug0415h.spr @15:04:20.21 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @15:04:21.88 Handoff.select :waste @15:04:22.00 ArS.seek empty @15:04:39.72 PinPort.seek :cartridge @15:04:45.45 LyS1.seek 1350ul @15:04:51.39 PV.seek :reagent @15:04:52.23 AirS.seek :tVent @15:04:54.30 delay 2 seconds @15:04:56.33 AirS.reconfigure EvacAirS @15:04:56.39 AirS.seek :evac @15:05:18.70 delay 15 seconds @15:05:33.73 AirS.reconfigure AirSconfig @15:05:33.78 AirS.seek :tVent @15:05:48.00 delay 2 seconds @15:05:50.03 AirS.reconfigure EvacAirS @15:05:50.08 AirS.seek :evac @15:06:12.40 delay 15 seconds @15:06:27.43 AirS.reconfigure AirSconfig @15:06:27.48 AirS.seek :tVent @15:06:41.70 delay 2 seconds @15:06:43.73 AirS.reconfigure EvacAirS @15:06:43.78 AirS.seek :evac @15:07:06.09 delay 15 seconds @15:07:21.11 AirS.reconfigure AirSconfig @15:07:21.17 AirS.seek :tVent @15:07:35.39 delay 2 seconds @15:07:37.42 AirS.reconfigure EvacAirS @15:07:37.47 AirS.seek :evac @15:07:59.78 delay 15 seconds @15:08:14.81 AirS.reconfigure AirSconfig @15:08:14.87 AirS.seek :tVent @15:08:29.09 delay 2 seconds @15:08:31.11 AirS.reconfigure EvacAirS @15:08:31.17 AirS.seek :evac @15:08:53.48 delay 15 seconds @15:09:08.50 delay 1:30 @15:10:38.53 AirS.reconfigure AirSconfig @15:10:38.58 AirS.seek :fVent @15:10:40.01 delay 15 seconds @15:10:55.03 LyS1.reconfigure SlowLyS1 @15:10:55.13 LyS1.seek 1050ul @15:11:25.17 delay 5 seconds @15:11:30.19 Handoff.select :blocked @15:11:30.35 LyS1.seek 450ul @15:12:30.37 delay 10 seconds @15:12:40.39 AirS.reconfigure SlowAirS @15:12:40.50 AirS.reconfigure to mix into 1.37806 parts LyS1 @15:12:40.83 AirS.seek evac @15:12:40.87 LyS1.seek 300ul @15:12:55.97 AirS.reconfigure AirSconfig @15:12:56.03 AirS.seek :full @15:13:10.24 LyS1.seek :empty @15:13:40.33 PuckHeater.seek 86.5C @15:14:53.04 PuckHeater.hold 15:00,86.5C @15:29:51.96 LyS1.reconfigure LyS1config @15:29:52.02 LyS1.seek 900ul @15:30:00.37 AirS.seek :tVent @15:30:00.45 delay 2 seconds @15:30:02.48 AirS.reconfigure HalfSpeedAirS @15:30:02.58 AirS.seek :evac @15:30:30.34 delay 15 seconds @15:30:45.37 AirS.reconfigure AirSconfig @15:30:45.42 AirS.seek :tVent @15:30:59.65 delay 2 seconds @15:31:01.68 AirS.reconfigure HalfSpeedAirS @15:31:01.74 AirS.seek :evac @15:31:29.51 delay 15 seconds @15:31:44.54 AirS.reconfigure AirSconfig @15:31:44.59 AirS.seek :tVent @15:31:58.81 delay 2 seconds @15:32:00.84 AirS.reconfigure HalfSpeedAirS @15:32:00.90 AirS.seek :evac @15:32:28.65 delay 15 seconds @15:32:43.67 delay 1:00 @15:33:43.69 Handoff.select :waste @15:33:43.80 delay 15 seconds @15:33:58.83 AirS.seek :fVent @15:34:01.02 delay 10 seconds @15:34:11.05 AirS.reconfigure AirSconfig @15:34:11.10 AirS.seek :evac @15:34:12.54 Lysis Recovered in LyS1 @15:34:12.55 Lysis Complete @15:34:12.56 Handoff.select :waste @15:34:12.59 LyS2.seek :full @15:34:12.67 LyS1.reconfigure DAmixLyS1 @15:34:12.73 LyS2.reconfigure DAmixLyS2 @15:34:13.14 LyS2.seek 1480ul @15:34:13.18 LyS1.seek 650ul @15:35:00.51 delay 5 seconds @15:35:05.53 Handoff.select :SPR @15:35:05.64 SPR.command \"start 1 14\" @15:35:06.72 Running Experiment-Rep #1 @15:35:06.73 delay 10 seconds @15:35:16.75 SPR.command \"start 1 0\" @15:35:19.61 Waiting up to 4:00 for SPR to enter state 1 @15:37:23.85 SPR.command \"hold_on\" @15:37:25.59 LyS2.seek 1130ul @15:37:25.62 LyS1.seek 300ul @15:38:31.83 delay 15 seconds @15:38:46.85 SPR.command \"hold_off\" @15:38:49.58 Waiting up to 25:00 for SPR to enter state 7 @15:58:53.96 Handoff.select :waste @15:58:54.08 LyS1.reconfigure LyS1config @15:58:54.15 LyS2.reconfigure LyS2config @15:58:54.44 Rep #1 SPR Analysis has Completed @15:58:54.89 Ended log /var/log/esp/spr18aug0415h.spr @15:58:54.90 SPR.command \"stats_off\" @15:58:57.04 -> Cmd.stop @15:58:57.13 Handoff.select :close @15:58:57.31 Gate.power :heat,:OFF @15:58:57.33 Gate.power :cartridge,:OFF @15:58:57.36 PV.seek :locked @15:58:58.25 PinPort.seek :clear @15:59:04.03 ArS.seek :clear @15:59:04.16 LyS1.seek :clear @15:59:04.29 LyS2.seek :clear @15:59:04.42 AirS.seek :clear @15:59:21.81 ArS.coast @15:59:21.86 LyS1.coast @15:59:21.91 LyS2.coast @15:59:21.96 AirS.coast @15:59:22.01 TV.coast @15:59:22.05 SP.coast @15:59:22.10 SC.coast @15:59:22.15 PV.coast @15:59:22.20 PinPort.coast @15:59:22.38 Gate.power :motor,:OFF @15:59:22.40 Gate.power :main,:OFF @15:59:22.42 Gate.power :core,:OFF @15:59:22.44 Gate.power :analytic1,:OFF @15:59:22.46 Gate.power :analytic2,:OFF @15:59:22.48 Gate.power :analytic3,:OFF @15:59:22.50 Safely stopped and ready to power off ##-- file closed 2018-08-05T08:22:38.034Z