##-- file opened 2018-08-30T00:25:18.958Z @20:24:21.26 -> showStatus @20:24:23.25 -> Cmd.loadCartridge @20:24:23.37 Gate.power :main,:ON,limit to 5000mA @20:24:23.39 Gate.power :analytic1,:ON,limit to 5000mA @20:24:23.41 Gate.power :analytic2,:ON,limit to 5000mA @20:24:23.43 Gate.power :analytic3,:ON,limit to 5000mA @20:24:23.57 Powering core bus @20:24:25.88 SPR Module 1, FW Rev. B, FPGA Rev. C @20:24:25.98 ArS.configure ArSconfig @20:24:26.08 LyS1.configure LyS1config @20:24:26.20 LyS2.configure LyS2config @20:24:26.32 AirS.configure AirSconfig @20:24:26.44 TV.configure TVconfig @20:24:26.56 SP.configure SPconfig @20:24:26.68 SC.configure SCconfig @20:24:26.79 PV.configure PVconfig @20:24:26.91 PinPort.configure PPconfig @20:24:27.04 Sea.configure SPconfig @20:24:27.09 All elf controllers running firmware version 4.5 @20:24:27.11 Gate.power :motor,:ON,limit to 5000mA @20:24:27.38 LyS1.reconfigure LyS1config @20:24:27.48 ArS.reconfigure ArSconfig @20:24:27.63 ArS.home.jog 0 @20:24:27.65 LyS1.home.jog 0 @20:24:27.84 PV.reconfigure PVconfig @20:24:27.90 TV.reconfigure TVconfig @20:24:27.94 LyS2.reconfigure LyS2config @20:24:27.99 PinPort.reconfigure PPconfig @20:24:28.09 AirS.reconfigure AirSconfig @20:24:28.20 PV.home.jog 0 @20:24:28.25 LyS2.home.jog 0 @20:24:28.29 PinPort.home.jog 0 @20:24:28.32 TV.home.jog 0 @20:24:28.45 AirS.home.jog 0 @20:24:28.73 ArS.reconfigure HomeMagSyringe @20:24:28.78 LyS1.reconfigure HomeSyringe @20:24:28.92 ArS.jog -234 @20:24:28.94 LyS1.jog -262 @20:24:29.11 PV.reconfigure HomePV @20:24:29.16 AirS.reconfigure HomeMagSyringe @20:24:29.20 LyS2.reconfigure HomeSyringe @20:24:29.23 PinPort.reconfigure HomePP @20:24:29.27 TV.reconfigure HomeTV @20:24:29.43 PV.jog -1474 @20:24:29.47 LyS2.jog -257 @20:24:29.51 PinPort.jog -111 @20:24:29.54 AirS.jog -313 @20:24:29.57 TV.jog -541 @20:24:29.77 LyS1.setRawPosition! -50 @20:24:29.79 ArS.setRawPosition! -50 @20:24:29.86 LyS1.seek :home @20:24:29.95 ArS.seek :home @20:24:30.35 AirS.setRawPosition! -50 @20:24:30.38 PinPort.setRawPosition! 0 @20:24:30.40 LyS2.setRawPosition! -50 @20:24:30.50 AirS.seek :home @20:24:30.58 PinPort.seek :home @20:24:30.61 LyS2.seek :home @20:24:30.84 PV.setRawPosition! -50 @20:24:30.87 ArS.reconfigure ArSconfig @20:24:30.89 PinPort.reconfigure PPconfig @20:24:30.93 LyS1.reconfigure LyS1config @20:24:31.07 PV.seek :home @20:24:31.23 AirS.reconfigure AirSconfig @20:24:31.26 LyS2.reconfigure LyS2config @20:24:31.43 PV.reconfigure PVconfig @20:24:31.54 TV.setRawPosition! 0 @20:24:31.58 TV.seek :home @20:24:31.68 TV.reconfigure TVconfig @20:24:31.83 TV.seek :clear @20:24:33.45 TV.coast @20:24:33.50 SC.reconfigure HomeSC @20:24:33.57 SC.home.jog 0 @20:24:34.32 SC.reconfigure SCconfig @20:24:34.38 SC.seek 28 @20:24:34.45 Selecting Cartridge 27 @20:24:34.75 SC.seek 27 @20:24:35.26 LyS2.seek :locked @20:24:35.38 PV.seek :locked @20:24:35.52 AirS.seek :locked @20:24:39.59 Powering cartridge bus @20:24:41.72 Gate.setIR 1750,5000,0,6 @20:24:41.84 Gate.setIR 0,5000,0,6 @20:24:41.88 Loaded Archive Cartridge @20:24:41.89 Gate.power :cartridge,:OFF @20:25:01.59 -> Cmd.startFiltering @20:25:01.83 Duration of filtering limited to 1:05:00 @20:25:02.08 Priming sample loop w/100ml, bypass w/10ml @20:25:02.16 SP.setPosition! 0ml @20:25:02.20 Intake.open @20:25:02.22 Exhaust.open @20:25:03.08 Sea @-42.9psia @20:25:03.10 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @20:25:08.89 Vacuum tare is 24% of full scale @20:25:08.90 PB.open @20:25:08.93 Exhaust.close @20:25:09.76 Sea @-42.9psia @20:25:09.84 SP.reconfigure SPprime @20:25:09.91 SP.seek 10ml,1:21 @20:25:20.76 SP.setPosition! 0ml @20:25:20.81 PB.close @20:25:20.83 Exhaust.open @20:25:21.65 Sea @-42.9psia @20:25:21.73 SP.reconfigure SPprime @20:25:21.80 SP.seek 100ml,8:51 @20:27:15.43 Intake.close @20:27:16.18 Pressurizing cartridge to 26psi (try #1 of 3) @20:27:16.19 SP.setPosition! 0ml @20:27:16.25 PB.open @20:27:17.02 TV.seek :crack @20:27:17.82 TV.seek :clear @20:27:18.62 Intake.open @20:27:20.08 PB.close @20:27:20.79 Exhaust.close @20:27:21.58 Sea @-42.9psia @20:27:21.66 SP.reconfigure SPcharge @20:27:21.73 SP.seek 2.3ml,42 seconds @20:27:32.58 Cartridge pressurized with 0.551ml to 26.6psi @20:27:32.96 delay 3 seconds @20:27:36.03 Cartridge at 22.5psi 3 seconds later @20:27:36.11 TV.seek :cartridge @20:27:37.93 TV.seek :clear @20:27:39.75 Sampling 1000.0ml @20:27:39.76 SP.setPosition! 0ml @20:27:39.81 Exhaust.open @20:27:40.59 Sea @-42.9psia @20:27:40.68 SP.reconfigure SPsample @20:27:40.75 SP.seek 1000ml,1:23:53 @20:27:41.70 Cartridge 27 sampling 0/1000ml at 0.17ml/s with 7.6psi vacuum @20:28:41.71 Cartridge 27 sampling 15/1000ml at 0.20ml/s with 24.8psi vacuum @20:28:58.28 Flow averaged 0.26ml/s over 1:12 @20:28:58.30 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @20:28:59.23 Relieved excess vacuum in 7.4 seconds @20:28:59.25 SP.seek 1000ml,1:22:20 @20:29:03.09 Flow estimate reduced to 0.21ml/s @20:29:03.11 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @20:29:04.04 Relieved excess vacuum in 1.7 seconds @20:29:04.06 SP.seek 1000ml,1:22:17 @20:29:07.43 Flow estimate reduced to 0.17ml/s @20:29:07.44 Intake.close @20:29:07.49 Exhaust.close @20:29:08.28 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @20:29:08.38 Sampled 19.8ml @20:29:08.47 TV.seek :bypass @20:29:10.08 TV.seek :clear @20:29:12.41 -> Cmd.startProcessing @20:29:12.66 PV.seek :reagent @20:29:13.54 Sample loop closed. Ready to Deliver Reagents @20:29:13.55 ArS.seek :sealed @20:29:17.82 ArS.seek :evac @20:29:31.75 delay 20 seconds @20:29:51.79 ArS.seek :clear @20:30:09.49 RNAlater soak for 10:00 @20:40:09.52 ArS.seek :tVent @20:40:12.08 delay 1 second @20:40:13.11 ArS.seek :evac @20:40:28.73 delay 10 seconds @20:40:38.76 Sample preserved and evacuated @20:40:39.77 -> Cmd.stop @20:40:39.92 PV.seek :locked @20:40:40.81 PinPort.seek :clear @20:40:40.95 ArS.seek :clear @20:40:41.13 LyS2.seek :clear @20:40:41.27 AirS.seek :clear @20:40:58.70 ArS.coast @20:40:58.75 LyS1.coast @20:40:58.80 LyS2.coast @20:40:58.85 AirS.coast @20:40:58.90 TV.coast @20:40:58.95 SP.coast @20:40:59.00 SC.coast @20:40:59.05 PV.coast @20:40:59.10 PinPort.coast @20:40:59.28 Gate.power :motor,:OFF @20:40:59.30 Gate.power :main,:OFF @20:40:59.32 Gate.power :core,:OFF @20:40:59.34 Gate.power :analytic1,:OFF @20:40:59.36 Gate.power :analytic2,:OFF @20:40:59.38 Gate.power :analytic3,:OFF @20:40:59.40 Safely stopped and ready to power off ##-- file closed 2018-08-30T00:53:06.154Z ##-- file opened 2018-08-30T00:53:06.156Z @20:52:05.89 -> showStatus @20:52:07.89 -> Cmd.loadCartridge @20:52:08.02 Gate.power :main,:ON,limit to 5000mA @20:52:08.03 Gate.power :analytic1,:ON,limit to 5000mA @20:52:08.05 Gate.power :analytic2,:ON,limit to 5000mA @20:52:08.07 Gate.power :analytic3,:ON,limit to 5000mA @20:52:08.22 Powering core bus @20:52:10.52 SPR Module 1, FW Rev. B, FPGA Rev. C @20:52:10.61 ArS.configure ArSconfig @20:52:10.71 LyS1.configure LyS1config @20:52:10.83 LyS2.configure LyS2config @20:52:10.94 AirS.configure AirSconfig @20:52:11.05 TV.configure TVconfig @20:52:11.17 SP.configure SPconfig @20:52:11.29 SC.configure SCconfig @20:52:11.41 PV.configure PVconfig @20:52:11.54 PinPort.configure PPconfig @20:52:11.66 Sea.configure SPconfig @20:52:11.71 All elf controllers running firmware version 4.5 @20:52:11.73 Gate.power :motor,:ON,limit to 5000mA @20:52:12.23 TV.reconfigure TVconfig @20:52:12.28 AirS.reconfigure AirSconfig @20:52:12.32 LyS1.reconfigure LyS1config @20:52:12.35 PinPort.reconfigure PPconfig @20:52:12.39 PV.reconfigure PVconfig @20:52:12.42 ArS.reconfigure ArSconfig @20:52:12.48 LyS2.reconfigure LyS2config @20:52:12.64 TV.home.jog 0 @20:52:12.66 AirS.home.jog 0 @20:52:12.71 LyS1.home.jog 0 @20:52:12.73 PV.home.jog 0 @20:52:12.81 ArS.home.jog 0 @20:52:12.87 PinPort.home.jog 0 @20:52:13.00 LyS2.home.jog 0 @20:52:13.52 TV.reconfigure HomeTV @20:52:13.62 PV.reconfigure HomePV @20:52:13.65 AirS.reconfigure HomeMagSyringe @20:52:13.68 PinPort.reconfigure HomePP @20:52:13.73 LyS1.reconfigure HomeSyringe @20:52:13.90 TV.jog -541 @20:52:13.93 AirS.jog -315 @20:52:13.96 PinPort.jog -111 @20:52:13.99 PV.jog -1475 @20:52:14.02 LyS1.jog -269 @20:52:14.24 LyS2.reconfigure HomeSyringe @20:52:14.26 ArS.reconfigure HomeMagSyringe @20:52:14.34 LyS2.jog -261 @20:52:14.38 ArS.jog -241 @20:52:14.51 PinPort.setRawPosition! 0 @20:52:14.56 PinPort.seek :home @20:52:14.90 AirS.setRawPosition! -50 @20:52:14.93 PinPort.reconfigure PPconfig @20:52:14.96 LyS1.setRawPosition! -50 @20:52:15.07 AirS.seek :home @20:52:15.12 LyS1.seek :home @20:52:15.24 LyS2.setRawPosition! -50 @20:52:15.26 ArS.setRawPosition! -50 @20:52:15.35 LyS2.seek :home @20:52:15.38 ArS.seek :home @20:52:15.54 PV.setRawPosition! -50 @20:52:15.57 AirS.reconfigure AirSconfig @20:52:15.60 LyS1.reconfigure LyS1config @20:52:15.73 PV.seek :home @20:52:15.86 ArS.reconfigure ArSconfig @20:52:15.89 LyS2.reconfigure LyS2config @20:52:16.05 TV.setRawPosition! 0 @20:52:16.10 TV.seek :home @20:52:16.20 PV.reconfigure PVconfig @20:52:16.22 TV.reconfigure TVconfig @20:52:16.47 TV.seek :clear @20:52:18.09 TV.coast @20:52:18.14 SC.reconfigure HomeSC @20:52:18.21 SC.home.jog 0 @20:52:18.97 SC.reconfigure SCconfig @20:52:19.03 SC.seek 27 @20:52:19.11 Selecting Cartridge 26 @20:52:19.42 SC.seek 26 @20:52:19.93 LyS2.seek :locked @20:52:20.05 PV.seek :locked @20:52:20.19 AirS.seek :locked @20:52:24.26 Powering cartridge bus @20:52:26.39 Gate.setIR 1750,5000,0,6 @20:52:26.51 Gate.setIR 0,5000,0,6 @20:52:26.57 Loaded Lyse-n-Go Cartridge @20:52:26.58 PuckHeater.configure HeatConfig @20:52:26.63 Gate.power :heat,:ON,limit to 5000mA @20:52:46.63 -> Cmd.startFiltering @20:52:46.85 Duration of filtering limited to 1:05:00 @20:52:47.11 Priming sample loop w/100ml, bypass w/10ml @20:52:47.18 SP.setPosition! 0ml @20:52:47.22 Intake.open @20:52:47.27 Exhaust.open @20:52:48.06 Sea @-42.9psia @20:52:48.08 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @20:52:53.61 Vacuum tare is 24% of full scale @20:52:53.62 PB.open @20:52:53.67 Exhaust.close @20:52:54.45 Sea @-42.9psia @20:52:54.53 SP.reconfigure SPprime @20:52:54.60 SP.seek 10ml,1:21 @20:53:05.46 SP.setPosition! 0ml @20:53:05.50 PB.close @20:53:05.53 Exhaust.open @20:53:06.36 Sea @-42.9psia @20:53:06.44 SP.reconfigure SPprime @20:53:06.50 SP.seek 100ml,8:51 @20:55:00.07 Intake.close @20:55:00.83 Pressurizing cartridge to 26psi (try #1 of 3) @20:55:00.84 SP.setPosition! 0ml @20:55:00.89 PB.open @20:55:01.69 TV.seek :crack @20:55:02.48 TV.seek :clear @20:55:03.28 Intake.open @20:55:04.73 PB.close @20:55:05.47 Exhaust.close @20:55:06.26 Sea @-42.9psia @20:55:06.34 SP.reconfigure SPcharge @20:55:06.41 SP.seek 2.3ml,42 seconds @20:55:14.83 Cartridge pressurized with 0.330ml to 26.1psi @20:55:15.06 delay 3 seconds @20:55:18.13 Cartridge at 19.6psi 3 seconds later @20:55:18.21 TV.seek :cartridge @20:55:20.03 TV.seek :clear @20:55:21.85 Sampling 1000.0ml @20:55:21.86 SP.setPosition! 0ml @20:55:21.91 Exhaust.open @20:55:22.66 Sea @-42.9psia @20:55:22.76 SP.reconfigure SPsample @20:55:22.82 SP.seek 1000ml,1:23:53 @20:55:23.77 Cartridge 26 sampling 0/1000ml at 0.17ml/s with 3.2psi vacuum @20:56:23.78 Cartridge 26 sampling 33/1000ml at 0.55ml/s with 23.9psi vacuum @20:57:23.77 Cartridge 26 sampling 65/1000ml at 0.52ml/s with 19.8psi vacuum @20:58:23.78 Cartridge 26 sampling 97/1000ml at 0.51ml/s with 23.0psi vacuum @20:59:23.78 Cartridge 26 sampling 128/1000ml at 0.51ml/s with 22.4psi vacuum @21:00:23.78 Cartridge 26 sampling 157/1000ml at 0.44ml/s with 23.4psi vacuum @21:01:23.78 Cartridge 26 sampling 184/1000ml at 0.41ml/s with 23.2psi vacuum @21:02:23.77 Cartridge 26 sampling 209/1000ml at 0.40ml/s with 21.7psi vacuum @21:03:23.78 Cartridge 26 sampling 230/1000ml at 0.27ml/s with 23.3psi vacuum @21:04:23.78 Cartridge 26 sampling 247/1000ml at 0.22ml/s with 24.9psi vacuum @21:05:23.78 Cartridge 26 sampling 261/1000ml at 0.20ml/s with 23.0psi vacuum @21:06:23.78 Cartridge 26 sampling 274/1000ml at 0.20ml/s with 27.3psi vacuum @21:07:23.78 Cartridge 26 sampling 286/1000ml at 0.20ml/s with 27.1psi vacuum @21:07:26.92 Flow averaged 0.40ml/s over 12:02 @21:07:26.94 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi @21:07:27.83 Relieved excess vacuum in 4 seconds @21:07:27.85 SP.seek 1000ml,59:57 @21:07:47.23 Flow averaged 0.29ml/s over 14 seconds @21:07:47.25 Waiting up to 18 seconds for vacuum to reduce from 30.0 to 18.0psi @21:07:47.87 Relieved excess vacuum in 6.9 seconds @21:07:47.90 SP.seek 1000ml,59:38 @21:07:54.83 Flow estimate reduced to 0.23ml/s @21:07:54.85 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi @21:07:55.47 Relieved excess vacuum in 4.5 seconds @21:07:55.50 SP.seek 1000ml,59:32 @21:08:02.40 Flow estimate reduced to 0.18ml/s @21:08:02.42 Intake.close @21:08:02.46 Exhaust.close @21:08:03.20 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @21:08:03.29 Sampled 293.3ml @21:08:03.38 TV.seek :bypass @21:08:04.99 TV.seek :clear @21:08:07.25 -> Cmd.startProcessing @21:08:09.04 SPR.command \"read_sequences\" @21:08:09.50 SPR.command \"stats_on\" @21:08:09.54 SPR.command \"start 0 1\" @21:08:11.55 Begun log /var/log/esp/spr18aug2921h.spr @21:08:12.58 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @21:08:14.24 Handoff.select :waste @21:08:14.36 ArS.seek empty @21:08:32.08 PinPort.seek :cartridge @21:08:37.79 LyS1.seek 1350ul @21:08:43.72 PV.seek :reagent @21:08:44.57 AirS.seek :tVent @21:08:46.65 delay 2 seconds @21:08:48.67 AirS.reconfigure EvacAirS @21:08:48.72 AirS.seek :evac @21:09:11.03 delay 15 seconds @21:09:26.06 AirS.reconfigure AirSconfig @21:09:26.11 AirS.seek :tVent @21:09:40.32 delay 2 seconds @21:09:42.35 AirS.reconfigure EvacAirS @21:09:42.40 AirS.seek :evac @21:10:04.69 delay 15 seconds @21:10:19.72 AirS.reconfigure AirSconfig @21:10:19.77 AirS.seek :tVent @21:10:33.98 delay 2 seconds @21:10:36.01 AirS.reconfigure EvacAirS @21:10:36.07 AirS.seek :evac @21:10:58.38 delay 15 seconds @21:11:13.41 AirS.reconfigure AirSconfig @21:11:13.73 AirS.seek :tVent @21:11:27.93 delay 2 seconds @21:11:29.97 AirS.reconfigure EvacAirS @21:11:30.02 AirS.seek :evac @21:11:52.31 delay 15 seconds @21:12:07.34 AirS.reconfigure AirSconfig @21:12:07.41 AirS.seek :tVent @21:12:21.61 delay 2 seconds @21:12:23.64 AirS.reconfigure EvacAirS @21:12:23.69 AirS.seek :evac @21:12:45.98 delay 15 seconds @21:13:01.00 delay 1:30 @21:14:31.03 AirS.reconfigure AirSconfig @21:14:31.08 AirS.seek :fVent @21:14:32.52 delay 15 seconds @21:14:47.54 LyS1.reconfigure SlowLyS1 @21:14:47.65 LyS1.seek 1050ul @21:15:17.67 delay 5 seconds @21:15:22.69 Handoff.select :blocked @21:15:22.85 LyS1.seek 450ul @21:16:22.80 delay 10 seconds @21:16:32.82 AirS.reconfigure SlowAirS @21:16:32.93 AirS.reconfigure to mix into 1.37806 parts LyS1 @21:16:33.36 AirS.seek evac @21:16:33.39 LyS1.seek 300ul @21:16:48.50 AirS.reconfigure AirSconfig @21:16:48.56 AirS.seek :full @21:17:02.76 LyS1.seek :empty @21:17:32.84 PuckHeater.seek 84C @21:18:31.87 PuckHeater.hold 15:00,84C @21:33:27.64 LyS1.reconfigure LyS1config @21:33:27.70 LyS1.seek 900ul @21:33:36.03 AirS.seek :tVent @21:33:36.12 delay 2 seconds @21:33:38.14 AirS.reconfigure HalfSpeedAirS @21:33:38.19 AirS.seek :evac @21:34:05.91 delay 15 seconds @21:34:20.94 AirS.reconfigure AirSconfig @21:34:21.07 AirS.seek :tVent @21:34:35.27 delay 2 seconds @21:34:37.30 AirS.reconfigure HalfSpeedAirS @21:34:37.35 AirS.seek :evac @21:35:05.09 delay 15 seconds @21:35:20.12 AirS.reconfigure AirSconfig @21:35:20.19 AirS.seek :tVent @21:35:34.39 delay 2 seconds @21:35:36.42 AirS.reconfigure HalfSpeedAirS @21:35:36.48 AirS.seek :evac @21:36:04.20 delay 15 seconds @21:36:19.23 delay 1:00 @21:37:19.25 Handoff.select :waste @21:37:19.37 delay 15 seconds @21:37:34.39 AirS.seek :fVent @21:37:36.58 delay 10 seconds @21:37:46.61 AirS.reconfigure AirSconfig @21:37:46.67 AirS.seek :evac @21:37:48.11 Lysis Recovered in LyS1 @21:37:48.13 Lysis Complete @21:37:48.15 Handoff.select :waste @21:37:48.18 LyS2.seek :full @21:37:48.27 LyS1.reconfigure DAmixLyS1 @21:37:48.33 LyS2.reconfigure DAmixLyS2 @21:37:48.71 LyS1.seek 650ul @21:37:48.74 LyS2.seek 1480ul @21:38:36.03 delay 5 seconds @21:38:41.05 Handoff.select :SPR @21:38:41.17 SPR.command \"start 2 14\" @21:38:42.53 Running Experiment 2 @21:38:42.54 delay 10 seconds @21:38:52.57 SPR.command \"start 2 0\" @21:38:55.89 Waiting up to 11:00 for SPR to enter state 1 @21:46:14.18 SPR.command \"hold_on\" @21:46:15.87 LyS1.seek 300ul @21:46:15.93 LyS2.seek 1130ul @21:47:22.06 delay 15 seconds @21:47:37.08 SPR.command \"hold_off\" @21:47:39.88 Waiting up to 25:00 for SPR to enter state 7 @21:59:16.37 Handoff.select :waste @21:59:16.50 LyS1.reconfigure LyS1config @21:59:16.56 LyS2.reconfigure LyS2config @21:59:16.62 Rep #1 SPR Analysis has Completed @21:59:17.31 Ended log /var/log/esp/spr18aug2921h.spr @21:59:17.32 SPR.command \"stats_off\" @21:59:19.38 -> Cmd.stop @21:59:19.47 Handoff.select :close @21:59:19.65 Gate.power :heat,:OFF @21:59:19.66 Gate.power :cartridge,:OFF @21:59:19.69 PV.seek :locked @21:59:20.59 PinPort.seek :clear @21:59:26.59 ArS.seek :clear @21:59:26.72 LyS1.seek :clear @21:59:26.85 LyS2.seek :clear @21:59:26.98 AirS.seek :clear @21:59:44.34 ArS.coast @21:59:44.39 LyS1.coast @21:59:44.44 LyS2.coast @21:59:44.48 AirS.coast @21:59:44.54 TV.coast @21:59:44.58 SP.coast @21:59:44.63 SC.coast @21:59:44.68 PV.coast @21:59:44.73 PinPort.coast @21:59:44.91 Gate.power :motor,:OFF @21:59:44.93 Gate.power :main,:OFF @21:59:44.95 Gate.power :core,:OFF @21:59:44.97 Gate.power :analytic1,:OFF @21:59:44.99 Gate.power :analytic2,:OFF @21:59:45.01 Gate.power :analytic3,:OFF @21:59:45.03 Safely stopped and ready to power off ##-- file closed 2018-08-30T02:12:43.437Z ##-- file opened 2018-08-30T02:12:43.439Z @22:11:35.79 -> showStatus @22:11:37.79 -> Cmd.loadCartridge @22:11:37.92 Gate.power :main,:ON,limit to 5000mA @22:11:37.93 Gate.power :analytic1,:ON,limit to 5000mA @22:11:37.96 Gate.power :analytic2,:ON,limit to 5000mA @22:11:37.97 Gate.power :analytic3,:ON,limit to 5000mA @22:11:38.11 Powering core bus @22:11:40.42 SPR Module 1, FW Rev. B, FPGA Rev. C @22:11:40.51 ArS.configure ArSconfig @22:11:40.61 LyS1.configure LyS1config @22:11:40.73 LyS2.configure LyS2config @22:11:40.83 AirS.configure AirSconfig @22:11:40.95 TV.configure TVconfig @22:11:41.07 SP.configure SPconfig @22:11:41.19 SC.configure SCconfig @22:11:41.31 PV.configure PVconfig @22:11:41.42 PinPort.configure PPconfig @22:11:41.54 Sea.configure SPconfig @22:11:41.60 All elf controllers running firmware version 4.5 @22:11:41.62 Gate.power :motor,:ON,limit to 5000mA @22:11:41.74 ArS.reconfigure ArSconfig @22:11:41.94 ArS.home.jog 0 @22:11:42.21 PinPort.reconfigure PPconfig @22:11:42.26 PV.reconfigure PVconfig @22:11:42.30 AirS.reconfigure AirSconfig @22:11:42.33 LyS1.reconfigure LyS1config @22:11:42.39 TV.reconfigure TVconfig @22:11:42.44 LyS2.reconfigure LyS2config @22:11:42.63 PinPort.home.jog 0 @22:11:42.66 AirS.home.jog 0 @22:11:42.69 PV.home.jog 0 @22:11:42.71 LyS1.home.jog 0 @22:11:42.75 TV.home.jog 0 @22:11:42.77 LyS2.home.jog 0 @22:11:43.23 ArS.reconfigure HomeMagSyringe @22:11:43.41 TV.reconfigure HomeTV @22:11:43.47 LyS2.reconfigure HomeSyringe @22:11:43.50 ArS.jog -225 @22:11:43.55 PinPort.reconfigure HomePP @22:11:43.57 PV.reconfigure HomePV @22:11:43.60 LyS1.reconfigure HomeSyringe @22:11:43.66 AirS.reconfigure HomeMagSyringe @22:11:43.86 TV.jog -545 @22:11:43.96 LyS2.jog -258 @22:11:44.00 PinPort.jog -112 @22:11:44.04 PV.jog -1478 @22:11:44.07 AirS.jog -320 @22:11:44.12 LyS1.jog -263 @22:11:44.35 ArS.setRawPosition! -50 @22:11:44.40 ArS.seek :home @22:11:44.83 PinPort.setRawPosition! 0 @22:11:44.86 LyS2.setRawPosition! -50 @22:11:44.88 ArS.reconfigure ArSconfig @22:11:44.98 PinPort.seek :home @22:11:45.05 LyS2.seek :home @22:11:45.21 PinPort.reconfigure PPconfig @22:11:45.23 AirS.setRawPosition! -50 @22:11:45.26 LyS1.setRawPosition! -50 @22:11:45.37 AirS.seek :home @22:11:45.42 LyS1.seek :home @22:11:45.58 PV.setRawPosition! -50 @22:11:45.59 LyS2.reconfigure LyS2config @22:11:45.68 PV.seek :home @22:11:45.81 AirS.reconfigure AirSconfig @22:11:45.84 LyS1.reconfigure LyS1config @22:11:45.98 TV.setRawPosition! 0 @22:11:46.03 TV.seek :home @22:11:46.13 PV.reconfigure PVconfig @22:11:46.15 TV.reconfigure TVconfig @22:11:46.42 TV.seek :clear @22:11:48.04 TV.coast @22:11:48.09 SC.reconfigure HomeSC @22:11:48.16 SC.home.jog 0 @22:11:48.91 SC.reconfigure SCconfig @22:11:48.97 SC.seek 26 @22:11:49.07 Selecting Cartridge 25 @22:11:49.37 SC.seek 25 @22:11:49.87 LyS2.seek :locked @22:11:49.99 PV.seek :locked @22:11:50.13 AirS.seek :locked @22:11:54.21 Powering cartridge bus @22:11:56.34 Gate.setIR 1750,5000,0,6 @22:11:56.47 Gate.setIR 0,5000,0,6 @22:11:56.50 Loaded Archive Cartridge @22:11:56.51 Gate.power :cartridge,:OFF @22:12:16.14 -> Cmd.startFiltering @22:12:16.37 Duration of filtering limited to 1:05:00 @22:12:16.62 Priming sample loop w/100ml, bypass w/10ml @22:12:16.70 SP.setPosition! 0ml @22:12:16.74 Intake.open @22:12:16.77 Exhaust.open @22:12:17.58 Sea @-42.9psia @22:12:17.60 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @22:12:23.38 Vacuum tare is 25% of full scale @22:12:23.40 PB.open @22:12:23.44 Exhaust.close @22:12:24.22 Sea @-42.9psia @22:12:24.31 SP.reconfigure SPprime @22:12:24.37 SP.seek 10ml,1:21 @22:12:35.22 SP.setPosition! 0ml @22:12:35.27 PB.close @22:12:35.29 Exhaust.open @22:12:36.09 Sea @-42.9psia @22:12:36.18 SP.reconfigure SPprime @22:12:36.24 SP.seek 100ml,8:51 @22:14:29.75 Intake.close @22:14:30.46 Pressurizing cartridge to 26psi (try #1 of 3) @22:14:30.48 SP.setPosition! 0ml @22:14:30.53 PB.open @22:14:31.32 TV.seek :crack @22:14:32.11 TV.seek :clear @22:14:32.90 Intake.open @22:14:34.33 PB.close @22:14:35.07 Exhaust.close @22:14:35.82 Sea @-42.9psia @22:14:35.90 SP.reconfigure SPcharge @22:14:35.97 SP.seek 2.3ml,42 seconds @22:14:45.62 Cartridge pressurized with 0.434ml to 26.2psi @22:14:45.85 delay 3 seconds @22:14:48.92 Cartridge at 17.9psi 3 seconds later @22:14:49.00 TV.seek :cartridge @22:14:50.83 TV.seek :clear @22:14:52.65 Sampling 1000.0ml @22:14:52.66 SP.setPosition! 0ml @22:14:52.70 Exhaust.open @22:14:53.46 Sea @-42.9psia @22:14:53.56 SP.reconfigure SPsample @22:14:53.62 SP.seek 1000ml,1:23:53 @22:14:54.58 Cartridge 25 sampling 0/1000ml at 0.17ml/s with 4.4psi vacuum @22:15:54.59 Cartridge 25 sampling 16/1000ml at 0.20ml/s with 22.8psi vacuum @22:16:06.52 Flow averaged 0.26ml/s over 1:12 @22:16:06.55 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @22:16:07.16 Relieved excess vacuum in 2.5 seconds @22:16:07.19 SP.seek 1000ml,1:22:21 @22:16:10.36 Flow estimate reduced to 0.21ml/s @22:16:10.38 Waiting up to 27 seconds for vacuum to reduce from 33.5 to 18.0psi @22:16:11.30 Relieved excess vacuum in 1 second @22:16:11.33 SP.seek 1000ml,1:22:19 @22:16:14.06 Flow estimate reduced to 0.16ml/s @22:16:14.08 Intake.close @22:16:14.12 Exhaust.close @22:16:14.90 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @22:16:15.00 Sampled 19.2ml @22:16:15.08 TV.seek :bypass @22:16:16.70 TV.seek :clear @22:16:18.97 -> Cmd.startProcessing @22:16:19.23 PV.seek :reagent @22:16:20.06 Sample loop closed. Ready to Deliver Reagents @22:16:20.08 ArS.seek :sealed @22:16:24.36 ArS.seek :evac @22:16:38.28 delay 20 seconds @22:16:58.57 ArS.seek :clear @22:17:16.27 RNAlater soak for 10:00 @22:27:16.29 ArS.seek :tVent @22:27:18.86 delay 1 second @22:27:19.89 ArS.seek :evac @22:27:35.51 delay 10 seconds @22:27:45.55 Sample preserved and evacuated @22:27:46.73 -> Cmd.stop @22:27:46.88 PV.seek :locked @22:27:47.77 PinPort.seek :clear @22:27:47.91 ArS.seek :clear @22:27:48.09 LyS2.seek :clear @22:27:48.22 AirS.seek :clear @22:28:05.66 ArS.coast @22:28:05.71 LyS1.coast @22:28:05.76 LyS2.coast @22:28:05.81 AirS.coast @22:28:05.86 TV.coast @22:28:05.91 SP.coast @22:28:05.96 SC.coast @22:28:06.01 PV.coast @22:28:06.06 PinPort.coast @22:28:06.24 Gate.power :motor,:OFF @22:28:06.25 Gate.power :main,:OFF @22:28:06.27 Gate.power :core,:OFF @22:28:06.29 Gate.power :analytic1,:OFF @22:28:06.32 Gate.power :analytic2,:OFF @22:28:06.34 Gate.power :analytic3,:OFF @22:28:06.36 Safely stopped and ready to power off ##-- file closed 2018-08-30T07:42:24.275Z ##-- file opened 2018-08-30T07:42:24.277Z @03:40:46.32 -> showStatus @03:40:48.32 -> Cmd.loadCartridge @03:40:48.44 Gate.power :main,:ON,limit to 5000mA @03:40:48.46 Gate.power :analytic1,:ON,limit to 5000mA @03:40:48.48 Gate.power :analytic2,:ON,limit to 5000mA @03:40:48.50 Gate.power :analytic3,:ON,limit to 5000mA @03:40:48.64 Powering core bus @03:40:50.94 SPR Module 1, FW Rev. B, FPGA Rev. C @03:40:51.04 ArS.configure ArSconfig @03:40:51.14 LyS1.configure LyS1config @03:40:51.26 LyS2.configure LyS2config @03:40:51.38 AirS.configure AirSconfig @03:40:51.49 TV.configure TVconfig @03:40:51.61 SP.configure SPconfig @03:40:51.73 SC.configure SCconfig @03:40:51.85 PV.configure PVconfig @03:40:51.97 PinPort.configure PPconfig @03:40:52.09 Sea.configure SPconfig @03:40:52.15 All elf controllers running firmware version 4.5 @03:40:52.17 Gate.power :motor,:ON,limit to 5000mA @03:40:52.33 ArS.reconfigure ArSconfig @03:40:52.60 ArS.home.jog 0 @03:40:52.73 PV.reconfigure PVconfig @03:40:52.79 TV.reconfigure TVconfig @03:40:52.82 LyS2.reconfigure LyS2config @03:40:52.88 AirS.reconfigure AirSconfig @03:40:52.98 LyS1.reconfigure LyS1config @03:40:53.02 PinPort.reconfigure PPconfig @03:40:53.21 TV.home.jog 0 @03:40:53.24 PV.home.jog 0 @03:40:53.27 LyS2.home.jog 0 @03:40:53.29 AirS.home.jog 0 @03:40:53.32 LyS1.home.jog 0 @03:40:53.35 PinPort.home.jog 0 @03:40:53.68 ArS.reconfigure HomeMagSyringe @03:40:53.85 PinPort.reconfigure HomePP @03:40:53.92 TV.reconfigure HomeTV @03:40:54.01 PV.reconfigure HomePV @03:40:54.05 ArS.jog -240 @03:40:54.21 PinPort.jog -111 @03:40:54.23 PV.jog -1474 @03:40:54.26 TV.jog -549 @03:40:54.45 LyS2.reconfigure HomeSyringe @03:40:54.47 LyS1.reconfigure HomeSyringe @03:40:54.50 AirS.reconfigure HomeMagSyringe @03:40:54.62 LyS1.jog -260 @03:40:54.67 AirS.jog -319 @03:40:54.70 LyS2.jog -260 @03:40:54.85 PinPort.setRawPosition! 0 @03:40:54.88 ArS.setRawPosition! -50 @03:40:54.94 PinPort.seek :home @03:40:55.25 ArS.seek :home @03:40:55.39 AirS.setRawPosition! -50 @03:40:55.42 LyS2.setRawPosition! -50 @03:40:55.44 LyS1.setRawPosition! -50 @03:40:55.48 PinPort.reconfigure PPconfig @03:40:55.61 AirS.seek :home @03:40:55.65 LyS1.seek :home @03:40:55.69 LyS2.seek :home @03:40:55.88 PV.setRawPosition! -50 @03:40:55.90 ArS.reconfigure ArSconfig @03:40:55.99 PV.seek :home @03:40:56.14 AirS.reconfigure AirSconfig @03:40:56.16 LyS2.reconfigure LyS2config @03:40:56.20 LyS1.reconfigure LyS1config @03:40:56.42 TV.setRawPosition! 0 @03:40:56.44 PV.reconfigure PVconfig @03:40:56.53 TV.seek :home @03:40:56.63 TV.reconfigure TVconfig @03:40:56.83 TV.seek :clear @03:40:58.45 TV.coast @03:40:58.50 SC.reconfigure HomeSC @03:40:58.57 SC.home.jog 0 @03:40:59.32 SC.reconfigure SCconfig @03:40:59.38 SC.seek 25 @03:40:59.48 Selecting Cartridge 24 @03:40:59.79 SC.seek 24 @03:41:00.28 LyS2.seek :locked @03:41:00.41 PV.seek :locked @03:41:00.54 AirS.seek :locked @03:41:04.62 Powering cartridge bus @03:41:06.75 Gate.setIR 1750,5000,0,6 @03:41:06.87 Gate.setIR 0,5000,0,6 @03:41:06.91 Loaded Archive Cartridge @03:41:06.92 Gate.power :cartridge,:OFF @03:41:26.65 -> Cmd.startFiltering @03:41:26.88 Duration of filtering limited to 1:05:00 @03:41:27.13 Priming sample loop w/100ml, bypass w/10ml @03:41:27.21 SP.setPosition! 0ml @03:41:27.25 Intake.open @03:41:27.27 Exhaust.open @03:41:28.13 Sea @-42.9psia @03:41:28.14 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @03:41:33.92 Vacuum tare is 24% of full scale @03:41:33.93 PB.open @03:41:33.98 Exhaust.close @03:41:34.78 Sea @-42.9psia @03:41:34.87 SP.reconfigure SPprime @03:41:34.93 SP.seek 10ml,1:21 @03:41:45.78 SP.setPosition! 0ml @03:41:45.83 PB.close @03:41:45.85 Exhaust.open @03:41:46.67 Sea @-42.9psia @03:41:46.75 SP.reconfigure SPprime @03:41:46.81 SP.seek 100ml,8:51 @03:43:40.80 Intake.close @03:43:41.54 Pressurizing cartridge to 26psi (try #1 of 3) @03:43:41.55 SP.setPosition! 0ml @03:43:41.60 PB.open @03:43:42.39 TV.seek :crack @03:43:43.18 TV.seek :clear @03:43:43.98 Intake.open @03:43:45.45 PB.close @03:43:46.16 Exhaust.close @03:43:46.93 Sea @-42.9psia @03:43:47.01 SP.reconfigure SPcharge @03:43:47.08 SP.seek 2.3ml,42 seconds @03:43:54.96 Cartridge pressurized with 0.289ml to 26.5psi @03:43:55.19 delay 3 seconds @03:43:58.26 Cartridge at 20.5psi 3 seconds later @03:43:58.34 TV.seek :cartridge @03:44:00.15 TV.seek :clear @03:44:01.96 Sampling 1000.0ml @03:44:01.97 SP.setPosition! 0ml @03:44:02.02 Exhaust.open @03:44:02.80 Sea @-42.9psia @03:44:02.89 SP.reconfigure SPsample @03:44:02.96 SP.seek 1000ml,1:23:53 @03:44:03.91 Cartridge 24 sampling 0/1000ml at 0.17ml/s with 6.1psi vacuum @03:45:03.92 Cartridge 24 sampling 24/1000ml at 0.34ml/s with 23.3psi vacuum @03:46:03.91 Cartridge 24 sampling 44/1000ml at 0.32ml/s with 22.0psi vacuum @03:47:03.92 Cartridge 24 sampling 60/1000ml at 0.24ml/s with 23.1psi vacuum @03:48:03.92 Cartridge 24 sampling 73/1000ml at 0.20ml/s with 24.0psi vacuum @03:48:36.58 Flow averaged 0.29ml/s over 4:32 @03:48:36.60 Waiting up to 18 seconds for vacuum to reduce from 30.0 to 18.0psi @03:48:37.22 Relieved excess vacuum in 3.2 seconds @03:48:37.24 SP.seek 1000ml,1:17:16 @03:48:43.93 Flow estimate reduced to 0.23ml/s @03:48:43.95 Waiting up to 20 seconds for vacuum to reduce from 30.9 to 18.0psi @03:48:44.57 Relieved excess vacuum in 4.2 seconds @03:48:44.60 SP.seek 1000ml,1:17:10 @03:48:50.00 Flow estimate reduced to 0.19ml/s @03:48:50.01 Intake.close @03:48:50.05 Exhaust.close @03:48:50.83 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @03:48:50.93 Sampled 81.4ml @03:48:51.01 TV.seek :bypass @03:48:52.62 TV.seek :clear @03:48:54.80 -> Cmd.startProcessing @03:48:55.32 PV.seek :reagent @03:48:56.17 Sample loop closed. Ready to Deliver Reagents @03:48:56.19 ArS.seek :sealed @03:49:00.47 ArS.seek :evac @03:49:14.40 delay 20 seconds @03:49:34.44 ArS.seek :clear @03:49:52.15 RNAlater soak for 10:00 @03:59:52.18 ArS.seek :tVent @03:59:54.75 delay 1 second @03:59:55.78 ArS.seek :evac @04:00:11.39 delay 10 seconds @04:00:21.42 Sample preserved and evacuated @04:00:22.53 -> Cmd.stop @04:00:22.67 PV.seek :locked @04:00:23.56 PinPort.seek :clear @04:00:23.69 ArS.seek :clear @04:00:23.88 LyS2.seek :clear @04:00:24.01 AirS.seek :clear @04:00:41.45 ArS.coast @04:00:41.50 LyS1.coast @04:00:41.55 LyS2.coast @04:00:41.60 AirS.coast @04:00:41.64 TV.coast @04:00:41.70 SP.coast @04:00:41.75 SC.coast @04:00:41.80 PV.coast @04:00:41.85 PinPort.coast @04:00:42.03 Gate.power :motor,:OFF @04:00:42.05 Gate.power :main,:OFF @04:00:42.07 Gate.power :core,:OFF @04:00:42.09 Gate.power :analytic1,:OFF @04:00:42.11 Gate.power :analytic2,:OFF @04:00:42.13 Gate.power :analytic3,:OFF @04:00:42.15 Safely stopped and ready to power off ##-- file closed 2018-08-30T08:14:10.673Z ##-- file opened 2018-08-30T08:14:10.675Z @04:12:29.80 -> showStatus @04:12:31.79 -> Cmd.loadCartridge @04:12:31.92 Gate.power :main,:ON,limit to 5000mA @04:12:31.93 Gate.power :analytic1,:ON,limit to 5000mA @04:12:31.95 Gate.power :analytic2,:ON,limit to 5000mA @04:12:31.98 Gate.power :analytic3,:ON,limit to 5000mA @04:12:32.12 Powering core bus @04:12:34.42 SPR Module 1, FW Rev. B, FPGA Rev. C @04:12:34.52 ArS.configure ArSconfig @04:12:34.62 LyS1.configure LyS1config @04:12:34.74 LyS2.configure LyS2config @04:12:34.84 AirS.configure AirSconfig @04:12:34.96 TV.configure TVconfig @04:12:35.08 SP.configure SPconfig @04:12:35.19 SC.configure SCconfig @04:12:35.31 PV.configure PVconfig @04:12:35.44 PinPort.configure PPconfig @04:12:35.56 Sea.configure SPconfig @04:12:35.61 All elf controllers running firmware version 4.5 @04:12:35.63 Gate.power :motor,:ON,limit to 5000mA @04:12:35.76 ArS.reconfigure ArSconfig @04:12:36.00 ArS.home.jog 0 @04:12:36.19 PV.reconfigure PVconfig @04:12:36.26 TV.reconfigure TVconfig @04:12:36.29 LyS2.reconfigure LyS2config @04:12:36.33 LyS1.reconfigure LyS1config @04:12:36.36 PinPort.reconfigure PPconfig @04:12:36.40 AirS.reconfigure AirSconfig @04:12:36.62 PV.home.jog 0 @04:12:36.67 TV.home.jog 0 @04:12:36.69 LyS1.home.jog 0 @04:12:36.73 PinPort.home.jog 0 @04:12:36.75 AirS.home.jog 0 @04:12:36.78 LyS2.home.jog 0 @04:12:37.23 ArS.reconfigure HomeMagSyringe @04:12:37.40 PV.reconfigure HomePV @04:12:37.44 TV.reconfigure HomeTV @04:12:37.50 ArS.jog -244 @04:12:37.56 LyS1.reconfigure HomeSyringe @04:12:37.58 PinPort.reconfigure HomePP @04:12:37.77 PV.jog -1477 @04:12:37.81 TV.jog -542 @04:12:37.84 LyS1.jog -262 @04:12:37.87 PinPort.jog -111 @04:12:38.07 LyS2.reconfigure HomeSyringe @04:12:38.08 AirS.reconfigure HomeMagSyringe @04:12:38.19 AirS.jog -314 @04:12:38.21 LyS2.jog -260 @04:12:38.33 PinPort.setRawPosition! 0 @04:12:38.34 ArS.setRawPosition! -50 @04:12:38.41 PinPort.seek :home @04:12:38.44 ArS.seek :home @04:12:38.85 AirS.setRawPosition! -50 @04:12:38.88 PinPort.reconfigure PPconfig @04:12:38.90 LyS1.setRawPosition! -50 @04:12:38.95 LyS2.setRawPosition! -50 @04:12:39.05 AirS.seek :home @04:12:39.13 LyS1.seek :home @04:12:39.18 LyS2.seek :home @04:12:39.39 PV.setRawPosition! -50 @04:12:39.40 ArS.reconfigure ArSconfig @04:12:39.48 PV.seek :home @04:12:39.63 LyS2.reconfigure LyS2config @04:12:39.65 LyS1.reconfigure LyS1config @04:12:39.68 AirS.reconfigure AirSconfig @04:12:39.90 TV.setRawPosition! 0 @04:12:39.92 PV.reconfigure PVconfig @04:12:40.00 TV.seek :home @04:12:40.11 TV.reconfigure TVconfig @04:12:40.34 TV.seek :clear @04:12:41.95 TV.coast @04:12:42.00 SC.reconfigure HomeSC @04:12:42.06 SC.home.jog 0 @04:12:42.81 SC.reconfigure SCconfig @04:12:42.87 SC.seek 24 @04:12:42.96 Selecting Cartridge 23 @04:12:43.26 SC.seek 23 @04:12:43.76 LyS2.seek :locked @04:12:43.88 PV.seek :locked @04:12:44.01 AirS.seek :locked @04:12:48.09 Powering cartridge bus @04:12:50.22 Gate.setIR 1750,5000,0,6 @04:12:50.35 Gate.setIR 0,5000,0,6 @04:12:50.40 Loaded Lyse-n-Go Cartridge @04:12:50.41 PuckHeater.configure HeatConfig @04:12:50.46 Gate.power :heat,:ON,limit to 5000mA @04:13:10.54 -> Cmd.startFiltering @04:13:10.78 Duration of filtering limited to 1:05:00 @04:13:11.02 Priming sample loop w/100ml, bypass w/10ml @04:13:11.10 SP.setPosition! 0ml @04:13:11.14 Intake.open @04:13:11.16 Exhaust.open @04:13:11.99 Sea @-42.9psia @04:13:12.01 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @04:13:17.79 Vacuum tare is 24% of full scale @04:13:17.81 PB.open @04:13:17.85 Exhaust.close @04:13:18.66 Sea @-42.9psia @04:13:18.75 SP.reconfigure SPprime @04:13:18.81 SP.seek 10ml,1:21 @04:13:29.66 SP.setPosition! 0ml @04:13:29.71 PB.close @04:13:29.73 Exhaust.open @04:13:30.54 Sea @-42.9psia @04:13:30.62 SP.reconfigure SPprime @04:13:30.69 SP.seek 100ml,8:51 @04:15:24.58 Intake.close @04:15:25.33 Pressurizing cartridge to 26psi (try #1 of 3) @04:15:25.35 SP.setPosition! 0ml @04:15:25.40 PB.open @04:15:26.19 TV.seek :crack @04:15:26.99 TV.seek :clear @04:15:27.78 Intake.open @04:15:29.24 PB.close @04:15:29.99 Exhaust.close @04:15:30.73 Sea @-42.9psia @04:15:30.82 SP.reconfigure SPcharge @04:15:30.88 SP.seek 2.3ml,42 seconds @04:15:43.11 Cartridge pressurized with 0.702ml to 27.1psi @04:15:43.49 delay 3 seconds @04:15:46.56 Cartridge at 21.5psi 3 seconds later @04:15:46.64 TV.seek :cartridge @04:15:48.47 TV.seek :clear @04:15:50.29 Sampling 1000.0ml @04:15:50.30 SP.setPosition! 0ml @04:15:50.34 Exhaust.open @04:15:51.09 Sea @-42.9psia @04:15:51.18 SP.reconfigure SPsample @04:15:51.25 SP.seek 1000ml,1:23:53 @04:15:52.20 Cartridge 23 sampling 0/1000ml at 0.17ml/s with 4.9psi vacuum @04:16:52.21 Cartridge 23 sampling 33/1000ml at 0.55ml/s with 23.1psi vacuum @04:17:52.21 Cartridge 23 sampling 65/1000ml at 0.55ml/s with 24.1psi vacuum @04:18:52.21 Cartridge 23 sampling 97/1000ml at 0.53ml/s with 23.9psi vacuum @04:19:52.22 Cartridge 23 sampling 129/1000ml at 0.52ml/s with 25.8psi vacuum @04:20:52.21 Cartridge 23 sampling 160/1000ml at 0.50ml/s with 23.4psi vacuum @04:21:52.21 Cartridge 23 sampling 190/1000ml at 0.52ml/s with 22.3psi vacuum @04:22:52.21 Cartridge 23 sampling 220/1000ml at 0.50ml/s with 22.4psi vacuum @04:23:52.21 Cartridge 23 sampling 249/1000ml at 0.49ml/s with 22.3psi vacuum @04:24:52.21 Cartridge 23 sampling 276/1000ml at 0.42ml/s with 23.8psi vacuum @04:25:52.21 Cartridge 23 sampling 302/1000ml at 0.41ml/s with 20.1psi vacuum @04:26:52.21 Cartridge 23 sampling 326/1000ml at 0.37ml/s with 19.9psi vacuum @04:27:52.21 Cartridge 23 sampling 347/1000ml at 0.36ml/s with 24.4psi vacuum @04:28:52.21 Cartridge 23 sampling 365/1000ml at 0.25ml/s with 25.6psi vacuum @04:29:52.47 Cartridge 23 sampling 382/1000ml at 0.23ml/s with 25.7psi vacuum @04:30:52.22 Cartridge 23 sampling 397/1000ml at 0.22ml/s with 21.8psi vacuum @04:31:52.22 Cartridge 23 sampling 410/1000ml at 0.20ml/s with 24.1psi vacuum @04:32:52.22 Cartridge 23 sampling 423/1000ml at 0.20ml/s with 24.7psi vacuum @04:33:52.22 Cartridge 23 sampling 435/1000ml at 0.20ml/s with 25.3psi vacuum @04:34:47.98 Flow averaged 0.39ml/s over 18:52 @04:34:48.01 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @04:34:48.63 Relieved excess vacuum in 6.2 seconds @04:34:48.66 SP.seek 1000ml,46:40 @04:34:52.20 Cartridge 23 sampling 447/1000ml at 0.20ml/s with 28.1psi vacuum @04:35:04.99 Flow averaged 0.23ml/s over 14 seconds @04:35:05.02 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @04:35:05.64 Relieved excess vacuum in 3.3 seconds @04:35:05.67 SP.seek 1000ml,46:25 @04:35:15.76 Flow estimate reduced to 0.19ml/s @04:35:15.78 Intake.close @04:35:15.83 Exhaust.close @04:35:16.60 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @04:35:16.69 Sampled 451.4ml @04:35:16.77 TV.seek :bypass @04:35:18.39 TV.seek :clear @04:35:20.52 -> Cmd.startProcessing @04:35:22.31 SPR.command \"read_sequences\" @04:35:22.94 SPR.command \"stats_on\" @04:35:22.98 SPR.command \"start 0 1\" @04:35:24.98 Begun log /var/log/esp/spr18aug3004h.spr @04:35:25.81 Handoff.select :waste @04:35:25.93 ArS.seek empty @04:35:43.65 PinPort.seek :cartridge @04:35:49.36 LyS1.seek 1350ul @04:35:55.30 PV.seek :reagent @04:35:56.13 AirS.seek :tVent @04:35:58.19 delay 2 seconds @04:36:00.22 AirS.reconfigure EvacAirS @04:36:00.27 AirS.seek :evac @04:36:22.58 delay 15 seconds @04:36:37.61 AirS.reconfigure AirSconfig @04:36:37.67 AirS.seek :tVent @04:36:51.88 delay 2 seconds @04:36:53.91 AirS.reconfigure EvacAirS @04:36:53.96 AirS.seek :evac @04:37:16.25 delay 15 seconds @04:37:31.28 AirS.reconfigure AirSconfig @04:37:31.34 AirS.seek :tVent @04:37:45.54 delay 2 seconds @04:37:47.57 AirS.reconfigure EvacAirS @04:37:47.63 AirS.seek :evac @04:38:09.92 delay 15 seconds @04:38:24.95 AirS.reconfigure AirSconfig @04:38:25.01 AirS.seek :tVent @04:38:39.24 delay 2 seconds @04:38:41.27 AirS.reconfigure EvacAirS @04:38:41.32 AirS.seek :evac @04:39:03.60 delay 15 seconds @04:39:18.63 AirS.reconfigure AirSconfig @04:39:18.69 AirS.seek :tVent @04:39:32.89 delay 2 seconds @04:39:34.92 AirS.reconfigure EvacAirS @04:39:34.97 AirS.seek :evac @04:39:57.26 delay 15 seconds @04:40:12.29 delay 1:30 @04:41:42.31 AirS.reconfigure AirSconfig @04:41:42.37 AirS.seek :fVent @04:41:43.80 delay 15 seconds @04:41:58.83 LyS1.reconfigure SlowLyS1 @04:41:58.93 LyS1.seek 1050ul @04:42:28.94 delay 5 seconds @04:42:33.96 Handoff.select :blocked @04:42:34.13 LyS1.seek 450ul @04:43:34.07 delay 10 seconds @04:43:44.10 AirS.reconfigure SlowAirS @04:43:44.44 AirS.reconfigure to mix into 1.37806 parts LyS1 @04:43:44.77 AirS.seek evac @04:43:44.80 LyS1.seek 300ul @04:43:59.89 AirS.reconfigure AirSconfig @04:43:59.95 AirS.seek :full @04:44:14.16 LyS1.seek :empty @04:44:44.22 PuckHeater.seek 84.1C @04:45:42.18 PuckHeater.hold 15:00,84.1C @05:00:43.30 LyS1.reconfigure LyS1config @05:00:43.37 LyS1.seek 900ul @05:00:51.72 AirS.seek :tVent @05:00:51.80 delay 2 seconds @05:00:53.82 AirS.reconfigure HalfSpeedAirS @05:00:53.87 AirS.seek :evac @05:01:21.59 delay 15 seconds @05:01:36.82 AirS.reconfigure AirSconfig @05:01:36.93 AirS.seek :tVent @05:01:51.12 delay 2 seconds @05:01:53.15 AirS.reconfigure HalfSpeedAirS @05:01:53.23 AirS.seek :evac @05:02:20.94 delay 15 seconds @05:02:35.97 AirS.reconfigure AirSconfig @05:02:36.03 AirS.seek :tVent @05:02:50.23 delay 2 seconds @05:02:52.26 AirS.reconfigure HalfSpeedAirS @05:02:52.32 AirS.seek :evac @05:03:20.03 delay 15 seconds @05:03:35.06 delay 1:00 @05:04:35.09 Handoff.select :waste @05:04:35.22 delay 15 seconds @05:04:50.25 AirS.seek :fVent @05:04:52.44 delay 10 seconds @05:05:02.47 AirS.reconfigure AirSconfig @05:05:02.53 AirS.seek :evac @05:05:03.97 Lysis Recovered in LyS1 @05:05:03.98 Lysis Complete @05:05:04.00 Handoff.select :waste @05:05:04.02 LyS2.seek :full @05:05:04.11 LyS1.reconfigure DAmixLyS1 @05:05:04.16 LyS2.reconfigure DAmixLyS2 @05:05:04.55 LyS1.seek 650ul @05:05:04.60 LyS2.seek 1480ul @05:05:51.89 delay 5 seconds @05:05:56.91 Handoff.select :SPR @05:05:57.04 SPR.command \"start 2 14\" @05:05:57.87 Running Experiment 2 @05:05:57.88 delay 10 seconds @05:06:07.89 SPR.command \"start 2 0\" @05:06:11.73 Waiting up to 11:00 for SPR to enter state 1 @05:13:30.45 SPR.command \"hold_on\" @05:13:32.16 LyS1.seek 300ul @05:13:32.20 LyS2.seek 1130ul @05:14:38.34 delay 15 seconds @05:14:53.36 SPR.command \"hold_off\" @05:14:56.16 Waiting up to 25:00 for SPR to enter state 7 @05:26:32.65 Handoff.select :waste @05:26:32.78 LyS1.reconfigure LyS1config @05:26:32.84 LyS2.reconfigure LyS2config @05:26:32.90 Rep #1 SPR Analysis has Completed @05:26:33.59 Ended log /var/log/esp/spr18aug3004h.spr @05:26:33.61 SPR.command \"stats_off\" @05:26:35.88 -> Cmd.stop @05:26:35.97 Handoff.select :close @05:26:36.15 Gate.power :heat,:OFF @05:26:36.16 Gate.power :cartridge,:OFF @05:26:36.19 PV.seek :locked @05:26:37.09 PinPort.seek :clear @05:26:42.86 ArS.seek :clear @05:26:43.22 LyS1.seek :clear @05:26:43.35 LyS2.seek :clear @05:26:43.50 AirS.seek :clear @05:27:00.84 ArS.coast @05:27:00.89 LyS1.coast @05:27:00.94 LyS2.coast @05:27:00.99 AirS.coast @05:27:01.04 TV.coast @05:27:01.09 SP.coast @05:27:01.14 SC.coast @05:27:01.19 PV.coast @05:27:01.24 PinPort.coast @05:27:01.43 Gate.power :motor,:OFF @05:27:01.44 Gate.power :main,:OFF @05:27:01.46 Gate.power :core,:OFF @05:27:01.48 Gate.power :analytic1,:OFF @05:27:01.50 Gate.power :analytic2,:OFF @05:27:01.53 Gate.power :analytic3,:OFF @05:27:01.54 Safely stopped and ready to power off ##-- file closed 2018-08-30T09:40:32.307Z ##-- file opened 2018-08-30T09:40:32.309Z @05:38:43.47 -> showStatus @05:38:45.47 -> Cmd.loadCartridge @05:38:45.59 Gate.power :main,:ON,limit to 5000mA @05:38:45.61 Gate.power :analytic1,:ON,limit to 5000mA @05:38:45.63 Gate.power :analytic2,:ON,limit to 5000mA @05:38:45.65 Gate.power :analytic3,:ON,limit to 5000mA @05:38:45.80 Powering core bus @05:38:48.09 SPR Module 1, FW Rev. B, FPGA Rev. C @05:38:48.19 ArS.configure ArSconfig @05:38:48.28 LyS1.configure LyS1config @05:38:48.40 LyS2.configure LyS2config @05:38:48.50 AirS.configure AirSconfig @05:38:48.62 TV.configure TVconfig @05:38:48.74 SP.configure SPconfig @05:38:48.86 SC.configure SCconfig @05:38:48.97 PV.configure PVconfig @05:38:49.09 PinPort.configure PPconfig @05:38:49.21 Sea.configure SPconfig @05:38:49.26 All elf controllers running firmware version 4.5 @05:38:49.28 Gate.power :motor,:ON,limit to 5000mA @05:38:49.78 TV.reconfigure TVconfig @05:38:49.85 AirS.reconfigure AirSconfig @05:38:49.88 LyS1.reconfigure LyS1config @05:38:49.92 PinPort.reconfigure PPconfig @05:38:49.95 PV.reconfigure PVconfig @05:38:49.99 LyS2.reconfigure LyS2config @05:38:50.02 ArS.reconfigure ArSconfig @05:38:50.24 AirS.home.jog 0 @05:38:50.27 TV.home.jog 0 @05:38:50.29 PinPort.home.jog 0 @05:38:50.32 LyS2.home.jog 0 @05:38:50.35 LyS1.home.jog 0 @05:38:50.37 PV.home.jog 0 @05:38:50.40 ArS.home.jog 0 @05:38:50.93 PV.reconfigure HomePV @05:38:51.00 AirS.reconfigure HomeMagSyringe @05:38:51.03 LyS2.reconfigure HomeSyringe @05:38:51.09 PinPort.reconfigure HomePP @05:38:51.15 TV.reconfigure HomeTV @05:38:51.35 AirS.jog -324 @05:38:51.39 LyS2.jog -258 @05:38:51.42 PV.jog -1478 @05:38:51.45 PinPort.jog -110 @05:38:51.47 TV.jog -549 @05:38:51.71 ArS.reconfigure HomeMagSyringe @05:38:51.73 LyS1.reconfigure HomeSyringe @05:38:51.81 ArS.jog -232 @05:38:51.85 LyS1.jog -261 @05:38:52.03 PinPort.setRawPosition! 0 @05:38:52.09 PinPort.seek :home @05:38:52.43 PinPort.reconfigure PPconfig @05:38:52.45 AirS.setRawPosition! -50 @05:38:52.48 LyS2.setRawPosition! -50 @05:38:52.58 AirS.seek :home @05:38:52.64 LyS2.seek :home @05:38:52.80 PV.setRawPosition! -50 @05:38:52.82 LyS1.setRawPosition! -50 @05:38:52.85 ArS.setRawPosition! -50 @05:38:52.93 PV.seek :home @05:38:53.01 ArS.seek :home @05:38:53.04 LyS1.seek :home @05:38:53.24 LyS2.reconfigure LyS2config @05:38:53.27 AirS.reconfigure AirSconfig @05:38:53.41 PV.reconfigure PVconfig @05:38:53.52 LyS1.reconfigure LyS1config @05:38:53.55 ArS.reconfigure ArSconfig @05:38:53.58 TV.setRawPosition! 0 @05:38:53.72 TV.seek :home @05:38:53.81 TV.reconfigure TVconfig @05:38:54.18 TV.seek :clear @05:38:55.81 TV.coast @05:38:55.86 SC.reconfigure HomeSC @05:38:55.92 SC.home.jog 0 @05:38:56.68 SC.reconfigure SCconfig @05:38:56.74 SC.seek 23 @05:38:56.83 Selecting Cartridge 22 @05:38:57.13 SC.seek 22 @05:38:57.64 LyS2.seek :locked @05:38:57.77 PV.seek :locked @05:38:57.91 AirS.seek :locked @05:39:01.98 Powering cartridge bus @05:39:04.11 Gate.setIR 1750,5000,0,6 @05:39:04.23 Gate.setIR 0,5000,0,6 @05:39:04.27 Loaded Archive Cartridge Gate.power :cartridge,:OFF @05:39:23.82 -> Cmd.startFiltering @05:39:24.04 Duration of filtering limited to 1:05:00 @05:39:24.29 Priming sample loop w/100ml, bypass w/10ml @05:39:24.36 SP.setPosition! 0ml @05:39:24.41 Intake.open @05:39:24.43 Exhaust.open @05:39:25.28 Sea @-42.9psia @05:39:25.29 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @05:39:31.07 Vacuum tare is 25% of full scale @05:39:31.09 PB.open @05:39:31.13 Exhaust.close @05:39:31.92 Sea @-42.9psia @05:39:32.01 SP.reconfigure SPprime @05:39:32.07 SP.seek 10ml,1:21 @05:39:42.93 SP.setPosition! 0ml @05:39:42.97 PB.close @05:39:43.00 Exhaust.open @05:39:43.83 Sea @-42.9psia @05:39:43.91 SP.reconfigure SPprime @05:39:43.97 SP.seek 100ml,8:51 @05:41:37.52 Intake.close @05:41:38.28 Pressurizing cartridge to 26psi (try #1 of 3) @05:41:38.29 SP.setPosition! 0ml @05:41:38.34 PB.open @05:41:39.12 TV.seek :crack @05:41:39.91 TV.seek :clear @05:41:40.71 Intake.open @05:41:42.17 PB.close @05:41:42.90 Exhaust.close @05:41:43.70 Sea @-42.9psia @05:41:43.79 SP.reconfigure SPcharge @05:41:43.85 SP.seek 2.3ml,42 seconds @05:41:51.96 Cartridge pressurized with 0.306ml to 26.3psi @05:41:52.19 delay 3 seconds @05:41:55.26 Cartridge at 20.3psi 3 seconds later @05:41:55.34 TV.seek :cartridge @05:41:57.16 TV.seek :clear @05:41:58.97 Sampling 1000.0ml @05:41:58.98 SP.setPosition! 0ml @05:41:59.03 Exhaust.open @05:41:59.79 Sea @-42.9psia @05:41:59.88 SP.reconfigure SPsample @05:41:59.94 SP.seek 1000ml,1:23:53 @05:42:00.90 Cartridge 22 sampling 0/1000ml at 0.17ml/s with 6.0psi vacuum @05:43:00.91 Cartridge 22 sampling 23/1000ml at 0.33ml/s with 20.5psi vacuum @05:44:00.90 Cartridge 22 sampling 42/1000ml at 0.32ml/s with 25.0psi vacuum @05:45:00.91 Cartridge 22 sampling 58/1000ml at 0.20ml/s with 23.3psi vacuum @05:46:00.91 Cartridge 22 sampling 71/1000ml at 0.20ml/s with 24.3psi vacuum @05:46:30.27 Flow averaged 0.29ml/s over 4:22 @05:46:30.29 Waiting up to 21 seconds for vacuum to reduce from 31.0 to 18.0psi @05:46:30.92 Relieved excess vacuum in 9.9 seconds @05:46:30.94 SP.seek 1000ml,1:17:28 @05:46:41.07 Flow estimate reduced to 0.24ml/s @05:46:41.10 Waiting up to 22 seconds for vacuum to reduce from 31.8 to 18.0psi @05:46:41.72 Relieved excess vacuum in 7.7 seconds @05:46:41.74 SP.seek 1000ml,1:17:18 @05:46:45.47 Flow estimate reduced to 0.19ml/s @05:46:45.49 Intake.close @05:46:45.53 Exhaust.close @05:46:46.30 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @05:46:46.40 Sampled 79.5ml @05:46:46.48 TV.seek :bypass @05:46:48.09 TV.seek :clear @05:46:50.35 -> Cmd.startProcessing @05:46:50.84 PV.seek :reagent @05:46:51.71 Sample loop closed. Ready to Deliver Reagents @05:46:51.73 ArS.seek :sealed @05:46:56.00 ArS.seek :evac @05:47:09.94 delay 20 seconds @05:47:29.98 ArS.seek :clear @05:47:47.69 RNAlater soak for 10:00 @05:57:47.73 ArS.seek :tVent @05:57:50.30 delay 1 second @05:57:51.33 ArS.seek :evac @05:58:06.95 delay 10 seconds @05:58:16.99 Sample preserved and evacuated @05:58:18.11 -> Cmd.stop @05:58:18.25 PV.seek :locked @05:58:19.15 PinPort.seek :clear @05:58:19.28 ArS.seek :clear @05:58:19.46 LyS2.seek :clear @05:58:19.59 AirS.seek :clear @05:58:37.01 ArS.coast @05:58:37.05 LyS1.coast @05:58:37.10 LyS2.coast @05:58:37.15 AirS.coast @05:58:37.20 TV.coast @05:58:37.25 SP.coast @05:58:37.30 SC.coast @05:58:37.35 PV.coast @05:58:37.41 PinPort.coast @05:58:37.59 Gate.power :motor,:OFF @05:58:37.61 Gate.power :main,:OFF @05:58:37.63 Gate.power :core,:OFF @05:58:37.65 Gate.power :analytic1,:OFF @05:58:37.67 Gate.power :analytic2,:OFF @05:58:37.69 Gate.power :analytic3,:OFF @05:58:37.71 Safely stopped and ready to power off ##-- file closed 2018-08-30T16:54:53.215Z