##-- file opened 2021-08-11T14:01:07.325Z @10:01:07.25 -> showStatus @10:01:09.27 -> Cmd.loadCartridge -4 @10:01:09.33 Gate.power :main,:ON,limit to 5000mA @10:01:09.35 Gate.power :analytic1,:ON,limit to 5000mA @10:01:09.37 Gate.power :analytic2,:ON,limit to 5000mA @10:01:09.39 Gate.power :analytic3,:ON,limit to 5000mA @10:01:09.86 Powering core bus @10:01:11.81 SPR Module 1, FW Rev. B, FPGA Rev. C @10:01:12.35 Archive.configure :default @10:01:12.48 Air.configure :default @10:01:12.58 Lysis1.configure :default @10:01:12.68 Lysis2.configure :default @10:01:12.81 Plunge.configure :default @10:01:12.93 SamplePump.configure :default @10:01:13.07 ExtraPump.configure :default @10:01:13.19 Toroid.configure :default @10:01:13.31 Carousel.configure :default @10:01:13.43 Pressure.configure :default @10:01:13.55 PinPort.configure :default @10:01:13.61 All elf controllers running firmware version 4.741 @10:01:13.64 Gate.power :motor,:ON,limit to 5000mA @10:01:13.87 Archive.in :default @10:01:14.11 Archive.home.jog 0 @10:01:14.28 PinPort.in :default @10:01:14.35 Toroid.in :default @10:01:14.39 Plunge.in :default @10:01:14.42 Lysis2.in :default @10:01:14.46 Air.in :default @10:01:14.54 Lysis1.in :default @10:01:14.68 PinPort.home.jog 0 @10:01:14.73 Toroid.home.jog 0 @10:01:14.76 Plunge.home.jog 0 @10:01:14.82 Lysis2.home.jog 0 @10:01:14.84 Air.home.jog 0 @10:01:14.88 Lysis1.home.jog 0 @10:01:15.23 Archive.in :homing @10:01:15.43 Toroid.in :homing @10:01:15.47 Plunge.in :homing @10:01:15.53 Archive.jog 1396 @10:01:15.58 PinPort.in :homing @10:01:15.63 Lysis2.in :homing @10:01:15.80 Plunge.jog 1550 @10:01:15.83 Toroid.jog 610 @10:01:15.86 PinPort.jog -109 @10:01:15.88 Lysis2.jog 1410 @10:01:16.08 Lysis1.in :homing @10:01:16.10 Air.in :homing @10:01:16.19 Lysis1.jog 1410 @10:01:16.23 Air.jog 1411 @10:01:16.33 PinPort.setRawPosition! 0 @10:01:16.37 PinPort.seek :home @10:01:16.46 PinPort.in :default @10:01:16.86 Archive.setRawPosition! 50 @10:01:16.91 Archive.seek :home @10:01:17.12 Plunge.setRawPosition! 50 @10:01:17.17 Plunge.seek :home @10:01:17.26 Archive.in :default @10:01:17.29 Lysis2.setRawPosition! 50 @10:01:17.41 Lysis2.seek :home @10:01:17.51 Lysis1.setRawPosition! 50 @10:01:17.53 Air.setRawPosition! 50 @10:01:17.56 Plunge.in :default @10:01:17.66 Lysis1.seek :home @10:01:17.72 Air.seek :home @10:01:17.84 Lysis2.in :default @10:01:18.01 Toroid.setRawPosition! 0 @10:01:18.07 Toroid.seek :home @10:01:18.17 Lysis1.in :default @10:01:18.20 Toroid.in :default @10:01:18.23 Air.in :default @10:01:18.69 Toroid.seek :clear @10:01:20.30 Carousel.in :default @10:01:20.36 Carousel.home.jog 0 @10:01:20.85 Carousel.in :default @10:01:20.91 Carousel.seek 29 @10:01:21.08 Selecting Cartridge 30 @10:01:21.32 Carousel.seek 30 @10:01:21.81 Lysis1.in :fast @10:01:21.87 Lysis1.seek :locked @10:01:24.46 Plunge.seek :locked @10:01:24.58 Air.in :fast @10:01:24.65 Air.seek :locked @10:01:27.24 Powering cartridge bus @10:01:29.86 Gate.setIR 1750,5000,0,6 @10:01:29.99 Gate.setIR 0,5000,0,6 @10:01:30.04 Gate.power :cartridge,:OFF @10:01:30.57 Mounted archive Cartridge @10:01:48.88 -> Cmd.startFiltering @10:01:49.34 Priming sample loop w/100ml, bypass w/10ml @10:01:49.35 SamplePump.setPosition! 0ml @10:01:49.39 Bypass.open @10:01:50.85 Intake.open @10:01:51.64 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:01:54.22 Vacuum tare is 51% of full scale @10:01:54.35 Pressure.in :default @10:01:54.41 Pressure.hold @10:01:54.58 SamplePump.in :prime @10:01:54.64 SamplePump.seek 10ml,59 seconds @10:02:03.90 Pressure.stop @10:02:03.95 SamplePump.setPosition! 0ml @10:02:04.00 Exhaust.open @10:02:05.44 Bypass.close @10:02:06.97 Pressure.in :default @10:02:07.03 Pressure.hold @10:02:07.25 SamplePump.in :prime @10:02:07.32 SamplePump.seek 100ml,5:16 @10:04:13.01 Pressure.stop @10:04:13.07 Bypass.open @10:04:13.80 Intake.close @10:04:14.64 Toroid.in :hold @10:04:14.70 Toroid.seek :crack @10:04:16.21 Toroid.seek :cartridge @10:04:17.30 Toroid.in :default @10:04:17.36 Toroid.seek :clear @10:04:19.33 Pressurizing cartridge to 26psi above 13.6psia @10:04:19.34 SamplePump.setPosition! 0ml @10:04:19.38 Intake.open @10:04:20.13 Bypass.close @10:04:20.90 Exhaust.close @10:04:21.69 Pressure.in :default @10:04:21.75 Pressure.hold @10:04:22.31 SamplePump.in :charge @10:04:22.39 SamplePump.seek 2.3ml,42 seconds @10:04:29.25 Pressure.stop @10:04:29.30 Intake.close @10:04:30.14 Pressurized cartridge with 0.207ml to 26.1psi @10:04:30.15 Isolated @33.9psia (20.3psi) @10:04:30.16 Verifying cartridge holds at least 0.75..0.85 of 33.9psia @10:04:30.18 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:04:34.80 Sampling 1000.0ml @10:04:34.82 SamplePump.setPosition! 0ml @10:04:34.87 Exhaust.open @10:04:35.62 Intake.open @10:04:36.50 Pressure.in :default @10:04:36.56 Pressure.hold @10:04:36.79 SamplePump.in :sample @10:04:36.86 SamplePump.seek 1000ml,1:23:53 @10:04:39.55 Cartridge 30 filtering 1/1000ml at 0.36ml/s with 26.7psi vacuum @10:05:39.54 Cartridge 30 filtering 23/1000ml at 0.40ml/s with 23.9psi vacuum @10:06:39.54 Cartridge 30 filtering 43/1000ml at 0.30ml/s with 26.8psi vacuum @10:07:39.54 Cartridge 30 filtering 63/1000ml at 0.29ml/s with 28.1psi vacuum @10:08:39.54 Cartridge 30 filtering 81/1000ml at 0.29ml/s with 21.5psi vacuum @10:09:39.54 Cartridge 30 filtering 98/1000ml at 0.28ml/s with 22.1psi vacuum @10:10:39.54 Cartridge 30 filtering 113/1000ml at 0.28ml/s with 21.3psi vacuum @10:11:39.54 Cartridge 30 filtering 128/1000ml at 0.20ml/s with 24.6psi vacuum @10:12:39.54 Cartridge 30 filtering 141/1000ml at 0.20ml/s with 23.3psi vacuum @10:13:39.54 Cartridge 30 filtering 154/1000ml at 0.20ml/s with 26.3psi vacuum @10:14:29.11 Flow averaged 0.28ml/s over 9:53 @10:14:29.14 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @10:14:29.96 Relieved excess vacuum in .9 seconds @10:14:29.99 SamplePump.seek 1000ml,1:10:14 @10:14:39.54 Cartridge 30 filtering 166/1000ml at 0.20ml/s with 25.8psi vacuum @10:14:49.55 Flow averaged 0.20ml/s over 20 seconds @10:14:49.58 Waiting up to 18 seconds for vacuum to reduce from 30.2 to 18.0psi @10:14:50.39 Relieved excess vacuum in .8 seconds @10:14:50.42 SamplePump.seek 1000ml,1:09:54 @10:14:52.34 Flow estimate reduced to 0.16ml/s @10:14:52.36 Intake.close @10:14:52.40 Exhaust.close @10:14:52.59 Toroid.in :hold @10:14:52.65 Toroid.seek :bypass @10:14:54.28 Toroid.in :default @10:14:54.34 Toroid.seek :clear @10:14:55.95 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @10:14:55.96 Pressure.stop @10:14:56.15 Sampled 168.1ml @10:14:58.35 -> Cmd.startProcessing @10:14:58.72 Plunge.seek :reagent @10:14:59.56 Sample loop closed. Ready to Deliver Reagents @10:14:59.57 Archive.seek :sealed @10:15:03.84 Archive.seek :evac @10:15:17.78 delay 1:00 @10:16:17.81 Archive.seek :clear @10:16:35.53 RNAlater soak for 10:00 @10:26:35.55 Archive.seek :tVent @10:26:38.13 delay 1 second @10:26:39.15 Archive.seek :evac @10:26:54.77 delay 10 seconds @10:27:04.79 Archive.seek :tVent @10:27:20.42 delay 1 second @10:27:21.44 Archive.seek :evac @10:27:37.05 delay 10 seconds @10:27:47.08 Sample preserved and evacuated @10:27:48.04 -> Cmd.stop @10:27:48.18 Carousel.seek 30 @10:27:48.35 Plunge.seek :locked @10:27:49.30 Archive.in :unlocking @10:27:49.43 Archive.seek :clear @10:27:49.47 Air.in :unlocking @10:27:49.62 Air.seek :clear @10:27:49.66 Lysis1.in :unlocking @10:27:49.78 Lysis1.seek :clear @10:27:53.68 Gate.power :motor,:OFF @10:27:53.70 Gate.power :core,:OFF @10:27:53.72 Gate.power :analytic1,:OFF @10:27:53.74 Gate.power :analytic2,:OFF @10:27:53.76 Gate.power :analytic3,:OFF @10:27:53.78 Gate.power :main,:OFF @10:27:53.81 Safely stopped and ready to power off ##-- file closed 2021-08-11T14:36:51.062Z ##-- file opened 2021-08-11T14:36:51.076Z @10:36:50.93 -> showStatus @10:36:52.97 -> Cmd.loadCartridge -18 @10:36:53.03 Gate.power :main,:ON,limit to 5000mA @10:36:53.05 Gate.power :analytic1,:ON,limit to 5000mA @10:36:53.07 Gate.power :analytic2,:ON,limit to 5000mA @10:36:53.10 Gate.power :analytic3,:ON,limit to 5000mA @10:36:53.61 Powering core bus @10:36:55.53 SPR Module 1, FW Rev. B, FPGA Rev. C @10:36:56.35 Archive.configure :default @10:36:56.47 Air.configure :default @10:36:56.58 Lysis1.configure :default @10:36:56.69 Lysis2.configure :default @10:36:56.81 Plunge.configure :default @10:36:56.93 SamplePump.configure :default @10:36:57.08 ExtraPump.configure :default @10:36:57.20 Toroid.configure :default @10:36:57.32 Carousel.configure :default @10:36:57.44 Pressure.configure :default @10:36:57.55 PinPort.configure :default @10:36:57.62 All elf controllers running firmware version 4.741 @10:36:57.65 Gate.power :motor,:ON,limit to 5000mA @10:36:57.78 Archive.in :default @10:36:58.03 Archive.home.jog 0 @10:36:58.26 Toroid.in :default @10:36:58.34 Plunge.in :default @10:36:58.38 Lysis1.in :default @10:36:58.45 Air.in :default @10:36:58.48 Lysis2.in :default @10:36:58.54 PinPort.in :default @10:36:58.66 Toroid.home.jog 0 @10:36:58.74 Lysis1.home.jog 0 @10:36:58.78 Plunge.home.jog 0 @10:36:58.80 Air.home.jog 0 @10:36:58.84 Lysis2.home.jog 0 @10:36:58.88 PinPort.home.jog 0 @10:36:59.29 Archive.in :homing @10:36:59.53 Toroid.in :homing @10:36:59.55 Plunge.in :homing @10:36:59.58 Air.in :homing @10:36:59.62 Archive.jog 1407 @10:36:59.66 PinPort.in :homing @10:36:59.69 Lysis1.in :homing @10:36:59.74 Lysis2.in :homing @10:36:59.90 Toroid.jog 616 @10:36:59.95 Air.jog 1410 @10:37:00.00 Plunge.jog 1571 @10:37:00.02 PinPort.jog -109 @10:37:00.05 Lysis1.jog 1411 @10:37:00.09 Lysis2.jog 1409 @10:37:00.41 PinPort.setRawPosition! 0 @10:37:00.45 PinPort.seek :home @10:37:00.54 PinPort.in :default @10:37:00.99 Archive.setRawPosition! 50 @10:37:01.04 Archive.seek :home @10:37:01.30 Air.setRawPosition! 50 @10:37:01.37 Air.seek :home @10:37:01.46 Plunge.setRawPosition! 50 @10:37:01.49 Lysis2.setRawPosition! 50 @10:37:01.52 Archive.in :default @10:37:01.56 Lysis1.setRawPosition! 50 @10:37:01.66 Plunge.seek :home @10:37:01.72 Lysis2.seek :home @10:37:01.81 Lysis1.seek :home @10:37:01.92 Air.in :default @10:37:02.03 Plunge.in :default @10:37:02.13 Lysis1.in :default @10:37:02.17 Lysis2.in :default @10:37:02.28 Toroid.setRawPosition! 0 @10:37:02.33 Toroid.seek :home @10:37:02.43 Toroid.in :default @10:37:02.60 Toroid.seek :clear @10:37:04.22 Carousel.in :default @10:37:04.28 Carousel.home.jog 0 @10:37:04.76 Carousel.in :default @10:37:04.82 Carousel.seek 30 @10:37:04.96 Selecting Cartridge 27 @10:37:05.21 Carousel.seek 27 @10:37:06.09 Lysis1.in :fast @10:37:06.15 Lysis1.seek :locked @10:37:08.75 Plunge.seek :locked @10:37:08.87 Air.in :fast @10:37:08.93 Air.seek :locked @10:37:11.53 Powering cartridge bus @10:37:14.15 Gate.setIR 1750,5000,0,6 @10:37:14.28 Gate.setIR 0,5000,0,6 @10:37:14.34 PuckHeater.configure :default @10:37:14.39 Gate.power :heat,:ON,limit to 5000mA @10:37:15.06 Mounted lysis Cartridge board#Q05065 @10:37:15.08 Using default puck heat loss curve for HeatingAccelInCarousel @10:37:33.36 -> Cmd.startFiltering @10:37:33.82 Priming sample loop w/100ml, bypass w/10ml @10:37:33.83 SamplePump.setPosition! 0ml @10:37:33.87 Bypass.open @10:37:35.37 Intake.open @10:37:36.18 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:37:38.76 Vacuum tare is 51% of full scale @10:37:38.88 Pressure.in :default @10:37:38.94 Pressure.hold @10:37:39.11 SamplePump.in :prime @10:37:39.18 SamplePump.seek 10ml,59 seconds @10:37:48.45 Pressure.stop @10:37:48.50 SamplePump.setPosition! 0ml @10:37:48.55 Exhaust.open @10:37:50.01 Bypass.close @10:37:51.56 Pressure.in :default @10:37:51.62 Pressure.hold @10:37:51.84 SamplePump.in :prime @10:37:51.90 SamplePump.seek 100ml,5:16 @10:39:57.45 Pressure.stop @10:39:57.51 Bypass.open @10:39:58.26 Intake.close @10:39:59.13 Toroid.in :hold @10:39:59.19 Toroid.seek :crack @10:40:00.71 Toroid.seek :cartridge @10:40:01.79 Toroid.in :default @10:40:01.85 Toroid.seek :clear @10:40:03.80 Pressurizing cartridge to 14psi above 12.7psia @10:40:03.81 SamplePump.setPosition! 0ml @10:40:03.85 Intake.open @10:40:04.61 Bypass.close @10:40:05.37 Exhaust.close @10:40:06.59 Pressure.in :default @10:40:06.64 Pressure.hold @10:40:06.82 SamplePump.in :charge @10:40:06.89 SamplePump.seek 2.3ml,41 seconds @10:40:17.40 Pressure.stop @10:40:17.45 Intake.close @10:40:18.27 Pressurized cartridge with 0.492ml to 15.0psi @10:40:18.28 Isolated @23.7psia (11.0psi) @10:40:18.29 Verifying cartridge holds at least 0.75..0.85 of 23.7psia @10:40:18.33 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:40:22.90 Cartridge pressure fell 8% to 21.7psia @10:40:22.98 Sampling 40.0ml @10:40:22.99 SamplePump.setPosition! 0ml @10:40:23.04 Exhaust.open @10:40:23.73 Intake.open @10:40:24.10 Pressure.in :default @10:40:24.16 Pressure.hold @10:40:24.39 SamplePump.in :sample @10:40:24.45 SamplePump.seek 40ml,3:11 @10:40:27.13 Cartridge 27 filtering 1/40ml at 0.36ml/s with 10.9psi vacuum @10:41:27.14 Cartridge 27 filtering 21/40ml at 0.31ml/s with 12.3psi vacuum @10:42:27.14 Cartridge 27 filtering 40/40ml at 0.31ml/s with 12.0psi vacuum @10:42:27.91 Pressure.stop @10:42:28.03 Intake.close @10:42:28.09 Exhaust.close @10:42:28.22 Toroid.in :hold @10:42:28.28 Toroid.seek :bypass @10:42:29.92 Toroid.in :default @10:42:30.02 Toroid.seek :clear @10:42:31.63 Sampled 40.0ml @10:42:33.15 -> Cmd.startProcessing @10:42:34.66 SPR.command \"read_sequences\" @10:42:34.94 SPR.command \"stats_on\" @10:42:34.98 SPR.command \"start 0 1\" @10:42:35.79 SPR.start /var/log/esp/spr21aug1110h.spr.bz2 @10:42:37.79 Handoff.select :waste @10:42:44.47 Syringe tare is 19% of full scale @10:42:44.49 PinPort.in :locking @10:42:44.56 PinPort.seek :locked @10:42:45.04 Archive.in :fast @10:42:45.08 Lysis2.in :fast @10:42:45.15 Lysis1.seek 1350uL @10:42:45.22 Archive.seek :full @10:42:45.27 Lysis2.seek :full @10:42:47.39 Plunge.seek :reagent @10:42:48.29 Evacuating puck with filter bubble point of 24psi @10:42:48.31 PinPort.seek :locked @10:42:48.39 Handoff.select :waste @10:42:54.98 Air syringe pumping up to 1 stroke to reach 8psi @10:42:54.99 Air stroke #1 of 1 @10:42:55.08 Air.in :pressurize @10:42:55.14 Air.seek :pressurized @10:43:06.39 1.1psi after pressurize @10:43:06.40 Air.in :compress @10:43:06.48 Air.seek :evac @10:43:10.57 1.1psi after compress @10:43:25.65 0.5psi after compress (stable) @10:43:25.66 Air.in :default @10:43:25.73 Air.hold @10:43:25.90 at 0.5psi Opened to waste @10:43:25.91 Handoff.select :blocked @10:43:26.05 Archive syringe pumping up to 8 strokes to reach 24psi @10:43:26.06 Archive stroke #1 of 8 @10:43:26.14 Archive.in :pressurize @10:43:26.20 Archive.seek :pressurized @10:43:38.95 1.2psi after pressurize @10:43:38.96 Archive.in :compress @10:43:39.04 Archive.seek :empty @10:43:54.95 15.0psi after compress @10:44:07.52 15.5psi after compress (stable) @10:44:07.54 Archive.in :gulp @10:44:07.61 Archive.seek :topVent @10:44:14.83 15.8psi after gulp @10:44:27.40 15.8psi after gulp (stable) @10:44:27.41 Archive stroke #2 of 8 @10:44:27.50 Archive.in :pressurize @10:44:27.56 Archive.seek :pressurized @10:44:40.34 15.8psi after pressurize @10:44:40.35 Archive.in :compress @10:44:40.43 Archive.seek :empty @10:44:52.02 Reached 24.1 of 24.0psi after pumping 3368uL (1.95 strokes) @10:44:52.03 Archive.in :default @10:44:52.10 Archive.hold @10:44:52.17 Handoff.select :waste @10:45:03.86 delay 1:00 @10:46:03.88 Handoff.select :blocked @10:46:26.55 Handoff.select :waste @10:46:26.67 0.4psi at evacEnd @10:46:26.68 Handoff.select :blocked @10:46:26.81 Archive.in :fast @10:46:26.88 Archive.seek :topVent @10:46:40.27 Stable @0.5psi after Handoff blocked @10:46:40.29 Archive syringe pumping up to 8 strokes to reach 16psi @10:46:40.31 Archive stroke #1 of 8 @10:46:40.41 Archive.in :pressurize @10:46:40.49 Archive.seek :pressurized @10:46:53.23 0.6psi after pressurize @10:46:53.24 Archive.in :compress @10:46:53.32 Archive.seek :empty @10:47:09.21 13.8psi after compress @10:47:21.78 13.9psi after compress (stable) @10:47:21.80 Archive.in :gulp @10:47:21.87 Archive.seek :topVent @10:47:29.07 14.2psi after gulp @10:47:41.64 14.1psi after gulp (stable) @10:47:41.65 Archive stroke #2 of 8 @10:47:41.74 Archive.in :pressurize @10:47:41.80 Archive.seek :pressurized @10:47:54.54 14.1psi after pressurize @10:47:54.56 Archive.in :compress @10:47:54.63 Archive.seek :empty @10:48:01.89 Reached 15.7 of 15.5psi after pumping 3276uL (1.89 strokes) @10:48:01.90 Archive.in :default @10:48:01.97 Archive.hold @10:48:02.03 Handoff.select :waste @10:48:09.23 delay 1:00 @10:49:09.25 Handoff.select :blocked @10:49:31.92 Handoff.select :waste @10:49:32.04 0.4psi at evacEnd @10:49:32.05 Air.seek :fullVent @10:49:33.51 Archive.in :fast @10:49:33.58 Archive.seek :topVent @10:49:40.07 Seawater evacuated, Presure above/below filter released. Lysis begins @10:49:40.18 Lysis1.in :slow @10:49:40.24 Lysis1.seek 1050uL @10:50:10.30 delay 5 seconds @10:50:15.32 Handoff.select :blocked @10:50:15.49 Lysis1.seek 450uL @10:51:15.50 delay 3 seconds @10:51:18.57 Air.in to mix into 1.37806 parts Lysis1 @10:51:18.97 Air.seek :evac @10:51:19.01 Lysis1.seek 300uL @10:51:34.13 Lysis1.seek :empty @10:52:04.25 Lysis buffer delivered @20.0psi @10:52:04.26 Polling Can every 2:00 @10:52:04.81 PuckHeater.configure :default #ambient 24.7C @10:52:04.89 PuckHeater.seek 79.6C @10:52:46.07 PuckHeater.hold 2:00,79.6C @10:54:45.85 Polling Can every 2:00 @10:54:46.28 PuckHeater.configure :default #ambient 24.8C @10:54:46.33 PuckHeater.hold 30 seconds,78.6C @10:55:16.44 Polling Can every 2:00 @10:55:16.99 PuckHeater.configure :default #ambient 24.8C @10:55:17.04 PuckHeater.hold 30 seconds,78.1C @10:55:47.15 Polling Can every 2:00 @10:55:47.69 PuckHeater.configure :default #ambient 24.8C @10:55:47.74 PuckHeater.hold 10:00,77.6C @11:05:46.16 Lysis complete. Syringe pressure @21.7psi @11:05:46.17 Handoff.select :blocked @11:05:46.22 Archive syringe pumping up to 5 strokes to reach 40psi Archive stroke #1 of 5 @11:05:46.31 Archive.in :pressurize @11:05:46.37 Archive.seek :pressurized @11:05:59.13 21.4psi after pressurize @11:05:59.15 Archive.in :compress @11:05:59.22 Archive.seek :empty @11:06:06.94 Reached 40.7 of 40.0psi after pumping 1556uL (0.90 strokes) @11:06:06.95 Archive.in :default @11:06:07.02 Archive.hold @11:06:07.09 delay 2 seconds @11:06:09.11 Archive.in :fast @11:06:09.17 Archive.seek :full @11:06:15.76 Lysis1.seek 138uL @11:06:29.64 delay 1 second @11:06:30.67 Archive.seek :full @11:06:30.69 Lysis1.seek :empty @11:06:44.69 Initial air pressure @45.5psi @11:06:44.75 Lysis1.in :extract @11:06:44.83 Lysis1.seek 600uL,nil @11:06:48.35 Archive syringe pumping up to 6 strokes to reach 25psi @11:06:48.36 Archive stroke #1 of 6 @11:06:48.44 Archive.in :pressurize @11:06:48.51 Archive.seek :pressurized @11:07:01.27 15.6psi after pressurize @11:07:01.28 Archive.in :compress @11:07:01.35 Archive.seek :empty @11:07:11.41 Syringe::LysatePlungerStuck in PROCESSING -- at < 511uL during Lysis Syringe #1 (LyS1) move @11:07:11.44 Lysis1.in :default @11:07:11.51 Lysis1.seek 476uL @11:07:12.24 Reached 28.0 of 25.0psi after pumping 1623uL (0.94 strokes) @11:07:12.25 Archive.in :default @11:07:12.32 Archive.hold @11:07:13.32 Lysis1.in :extract @11:07:13.40 Lysis1.seek 600uL,nil @11:07:15.85 Initial 600uL lysate @20.6psi @11:07:15.86 Handoff.select :blocked @11:07:15.96 Initial air pressure @22.4psi @11:07:16.03 Lysis1.seek 825uL,nil @11:07:18.90 Archive syringe pumping up to 6 strokes to reach 17psi @11:07:18.91 Archive stroke #1 of 6 @11:07:19.00 Archive.in :compress @11:07:19.07 Archive.seek :empty @11:07:20.79 Archive.in :default @11:07:20.84 Archive.hold @11:07:21.13 Recovered 825uL lysate @17.1psi @11:07:21.14 Air.in :halfSpeed @11:07:21.21 Air.seek :fullVent @11:07:23.46 Puck Top Vented, bottom @5.0psi @11:07:23.47 Handoff.select :waste @11:07:23.59 delay 5 seconds @11:07:28.65 Handoff open to waste, bottom @0.1psi @11:07:28.66 Air.in :default @11:07:28.73 Air.seek :evac @11:07:30.17 Lysis2.in :DAmix @11:07:30.27 Lysis1.in :DAmix @11:07:30.82 Lysis2.seek 1480uL @11:07:30.86 Lysis1.seek 575uL @11:08:05.92 delay 5 seconds @11:08:10.94 Handoff.select SPR @11:08:11.07 SPR.command \"start 2 14\" @11:08:11.97 SPR stabilized @11:08:12.10 delay 10 seconds @11:08:22.12 SPR.command \"start 2 0\" @11:08:25.33 Waiting up to 11:00 for SPR to enter state 1 @11:10:22.98 SPR.command \"hold_on\" @11:10:24.39 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @11:10:24.83 Lysis2.seek 1180uL @11:10:24.90 Lysis1.seek 275uL @11:11:06.91 delay 15 seconds @11:11:21.93 SPR.command \"hold_off\" @11:11:25.04 Waiting up to 38:00 for SPR to enter state 7 @11:35:22.17 SPR analysis completed @11:35:26.05 Ended log spr21aug1110h.spr.bz2 @11:35:26.06 SPR.command \"stats_off\" @11:35:27.70 -> Cmd.stop @11:35:27.71 Archive.stop @11:35:27.83 Handoff.select :close @11:35:27.96 Gate.power :heat,:OFF @11:35:27.98 Gate.power :cartridge,:OFF @11:35:28.07 Carousel.seek 27 @11:35:28.24 Plunge.seek :locked @11:35:29.13 PinPort.in :unlocking @11:35:29.19 PinPort.seek :clear @11:35:29.74 Archive.in :unlocking @11:35:29.87 Air.in :unlocking @11:35:29.91 Archive.seek :clear @11:35:30.06 Air.seek :clear @11:35:30.18 Lysis1.in :unlocking @11:35:30.26 Lysis1.seek :clear @11:35:30.33 Lysis2.in :unlocking @11:35:30.40 Lysis2.seek :clear @11:35:34.00 Gate.power :motor,:OFF @11:35:34.02 Gate.power :core,:OFF @11:35:34.04 Gate.power :analytic1,:OFF @11:35:34.06 Gate.power :analytic2,:OFF @11:35:34.08 Gate.power :analytic3,:OFF @11:35:34.10 Gate.power :main,:OFF @11:35:34.12 Safely stopped and ready to power off ##-- file closed 2021-08-11T19:44:39.240Z ##-- file opened 2021-08-11T19:44:39.242Z @15:44:39.02 -> showStatus @15:44:41.04 -> Cmd.loadCartridge -4 @15:44:41.10 Gate.power :main,:ON,limit to 5000mA @15:44:41.12 Gate.power :analytic1,:ON,limit to 5000mA @15:44:41.14 Gate.power :analytic2,:ON,limit to 5000mA @15:44:41.17 Gate.power :analytic3,:ON,limit to 5000mA @15:44:41.68 Powering core bus @15:44:43.61 SPR Module 1, FW Rev. B, FPGA Rev. C @15:44:44.43 Archive.configure :default @15:44:44.56 Air.configure :default @15:44:44.66 Lysis1.configure :default @15:44:44.76 Lysis2.configure :default @15:44:44.88 Plunge.configure :default @15:44:45.01 SamplePump.configure :default @15:44:45.15 ExtraPump.configure :default @15:44:45.27 Toroid.configure :default @15:44:45.39 Carousel.configure :default @15:44:45.52 Pressure.configure :default @15:44:45.64 PinPort.configure :default @15:44:45.70 All elf controllers running firmware version 4.741 @15:44:45.73 Gate.power :motor,:ON,limit to 5000mA @15:44:45.89 Archive.in :default @15:44:46.18 Archive.home.jog 0 @15:44:46.32 Toroid.in :default @15:44:46.40 Plunge.in :default @15:44:46.43 Lysis1.in :default @15:44:46.48 Air.in :default @15:44:46.53 PinPort.in :default @15:44:46.58 Lysis2.in :default @15:44:46.75 Toroid.home.jog 0 @15:44:46.77 Plunge.home.jog 0 @15:44:46.82 Lysis1.home.jog 0 @15:44:46.87 PinPort.home.jog 0 @15:44:46.91 Lysis2.home.jog 0 @15:44:46.94 Air.home.jog 0 @15:44:47.27 Archive.in :homing @15:44:47.50 PinPort.in :homing @15:44:47.53 Toroid.in :homing @15:44:47.62 Archive.jog 1388 @15:44:47.66 Plunge.in :homing @15:44:47.68 Lysis1.in :homing @15:44:47.84 Toroid.jog 614 @15:44:47.88 PinPort.jog -108 @15:44:47.92 Plunge.jog 1559 @15:44:47.96 Lysis1.jog 1411 @15:44:48.14 Air.in :homing @15:44:48.16 Lysis2.in :homing @15:44:48.29 PinPort.setRawPosition! 0 @15:44:48.31 Lysis2.jog 1413 @15:44:48.33 Air.jog 1413 @15:44:48.43 PinPort.seek :home @15:44:48.54 PinPort.in :default @15:44:48.90 Archive.setRawPosition! 50 @15:44:48.94 Archive.seek :home @15:44:49.18 Lysis1.setRawPosition! 50 @15:44:49.24 Lysis1.seek :home @15:44:49.34 Archive.in :default @15:44:49.37 Plunge.setRawPosition! 50 @15:44:49.45 Plunge.seek :home @15:44:49.57 Lysis2.setRawPosition! 50 @15:44:49.60 Lysis1.in :default @15:44:49.63 Air.setRawPosition! 50 @15:44:49.71 Lysis2.seek :home @15:44:49.78 Air.seek :home @15:44:49.90 Plunge.in :default @15:44:49.99 Toroid.setRawPosition! 0 @15:44:50.03 Toroid.seek :home @15:44:50.16 Toroid.in :default @15:44:50.18 Lysis2.in :default @15:44:50.21 Air.in :default @15:44:50.66 Toroid.seek :clear @15:44:52.27 Carousel.in :default @15:44:52.33 Carousel.home.jog 0 @15:44:52.82 Carousel.in :default @15:44:52.88 Carousel.seek 27 @15:44:53.03 Selecting Cartridge 28 @15:44:53.27 Carousel.seek 28 @15:44:53.76 Lysis1.in :fast @15:44:53.82 Lysis1.seek :locked @15:44:56.41 Plunge.seek :locked @15:44:56.55 Air.in :fast @15:44:56.61 Air.seek :locked @15:44:59.21 Powering cartridge bus @15:45:01.83 Gate.setIR 1750,5000,0,6 @15:45:01.96 Gate.setIR 0,5000,0,6 @15:45:02.01 Gate.power :cartridge,:OFF @15:45:02.54 Mounted archive Cartridge @15:45:20.64 -> Cmd.startFiltering @15:45:21.10 Priming sample loop w/100ml, bypass w/10ml @15:45:21.11 SamplePump.setPosition! 0ml @15:45:21.15 Bypass.open @15:45:22.64 Intake.open @15:45:23.44 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:45:26.00 Vacuum tare is 51% of full scale @15:45:26.13 Pressure.in :default @15:45:26.18 Pressure.hold @15:45:26.35 SamplePump.in :prime @15:45:26.42 SamplePump.seek 10ml,59 seconds @15:45:35.68 Pressure.stop @15:45:35.73 SamplePump.setPosition! 0ml @15:45:35.78 Exhaust.open @15:45:37.27 Bypass.close @15:45:38.81 Pressure.in :default @15:45:38.87 Pressure.hold @15:45:39.09 SamplePump.in :prime @15:45:39.15 SamplePump.seek 100ml,5:16 @15:47:44.66 Pressure.stop @15:47:44.72 Bypass.open @15:47:45.44 Intake.close @15:47:46.30 Toroid.in :hold @15:47:46.36 Toroid.seek :crack @15:47:47.87 Toroid.seek :cartridge @15:47:48.95 Toroid.in :default @15:47:49.01 Toroid.seek :clear @15:47:50.97 Pressurizing cartridge to 26psi above 12.9psia @15:47:50.98 SamplePump.setPosition! 0ml @15:47:51.02 Intake.open @15:47:51.79 Bypass.close @15:47:52.51 Exhaust.close @15:47:53.71 Pressure.in :default @15:47:53.78 Pressure.hold @15:47:53.95 SamplePump.in :charge @15:47:54.02 SamplePump.seek 2.3ml,42 seconds @15:48:01.56 Pressure.stop @15:48:01.62 Intake.close @15:48:02.48 Pressurized cartridge with 0.252ml to 27.3psi @15:48:02.49 Isolated @33.9psia (21.0psi) @15:48:02.50 Verifying cartridge holds at least 0.75..0.85 of 33.9psia @15:48:02.53 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:48:07.15 Sampling 1000.0ml @15:48:07.17 SamplePump.setPosition! 0ml @15:48:07.22 Exhaust.open @15:48:07.93 Intake.open @15:48:08.80 Pressure.in :default @15:48:08.86 Pressure.hold @15:48:09.09 SamplePump.in :sample @15:48:09.15 SamplePump.seek 1000ml,1:23:53 @15:48:11.84 Cartridge 28 filtering 1/1000ml at 0.40ml/s with 24.3psi vacuum @15:49:11.84 Cartridge 28 filtering 23/1000ml at 0.33ml/s with 24.4psi vacuum @15:50:11.84 Cartridge 28 filtering 43/1000ml at 0.35ml/s with 20.3psi vacuum @15:51:11.84 Cartridge 28 filtering 63/1000ml at 0.28ml/s with 22.0psi vacuum @15:52:11.84 Cartridge 28 filtering 81/1000ml at 0.34ml/s with 22.3psi vacuum @15:53:11.84 Cartridge 28 filtering 99/1000ml at 0.29ml/s with 22.9psi vacuum @15:54:11.84 Cartridge 28 filtering 115/1000ml at 0.28ml/s with 21.8psi vacuum @15:55:11.84 Cartridge 28 filtering 130/1000ml at 0.20ml/s with 24.4psi vacuum @15:56:11.84 Cartridge 28 filtering 144/1000ml at 0.23ml/s with 22.2psi vacuum @15:57:11.84 Cartridge 28 filtering 157/1000ml at 0.24ml/s with 24.0psi vacuum @15:58:11.84 Cartridge 28 filtering 170/1000ml at 0.20ml/s with 28.3psi vacuum @15:59:01.47 Flow averaged 0.28ml/s over 10:53 @15:59:01.50 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @15:59:02.08 Relieved excess vacuum in .6 seconds @15:59:02.10 SamplePump.seek 1000ml,1:08:54 @15:59:11.84 Cartridge 28 filtering 182/1000ml at 0.20ml/s with 23.1psi vacuum @15:59:20.28 Flow averaged 0.20ml/s over 19 seconds @15:59:20.31 Waiting up to 16 seconds for vacuum to reduce from 29.3 to 18.0psi @15:59:20.88 Relieved excess vacuum in .6 seconds @15:59:20.91 SamplePump.seek 1000ml,1:08:36 @15:59:25.79 Flow estimate reduced to 0.16ml/s @15:59:25.81 Intake.close @15:59:25.86 Exhaust.close @15:59:26.05 Toroid.in :hold @15:59:26.11 Toroid.seek :bypass @15:59:27.73 Toroid.in :default @15:59:27.79 Toroid.seek :clear @15:59:29.40 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @15:59:29.41 Pressure.stop @15:59:29.60 Sampled 184.3ml @15:59:31.50 -> Cmd.startProcessing @15:59:31.88 Plunge.seek :reagent @15:59:32.71 Sample loop closed. Ready to Deliver Reagents @15:59:32.72 Archive.seek :sealed @15:59:36.99 Archive.seek :evac @15:59:50.92 delay 1:00 @16:00:50.94 Archive.seek :clear @16:01:08.66 RNAlater soak for 10:00 @16:11:08.69 Archive.seek :tVent @16:11:11.27 delay 1 second @16:11:12.29 Archive.seek :evac @16:11:27.90 delay 10 seconds @16:11:37.93 Archive.seek :tVent @16:11:53.54 delay 1 second @16:11:54.56 Archive.seek :evac @16:12:10.17 delay 10 seconds @16:12:20.20 Sample preserved and evacuated @16:12:21.14 -> Cmd.stop @16:12:21.28 Carousel.seek 28 @16:12:21.45 Plunge.seek :locked @16:12:22.40 Archive.in :unlocking @16:12:22.53 Archive.seek :clear @16:12:22.61 Air.in :unlocking @16:12:22.71 Air.seek :clear @16:12:22.79 Lysis1.in :unlocking @16:12:22.90 Lysis1.seek :clear @16:12:26.70 Gate.power :motor,:OFF @16:12:26.72 Gate.power :core,:OFF @16:12:26.74 Gate.power :analytic1,:OFF @16:12:26.76 Gate.power :analytic2,:OFF @16:12:26.78 Gate.power :analytic3,:OFF @16:12:26.80 Gate.power :main,:OFF @16:12:26.82 Safely stopped and ready to power off ##-- file closed 2021-08-11T20:21:24.280Z ##-- file opened 2021-08-11T20:21:24.282Z @16:21:24.30 -> showStatus @16:21:26.32 -> Cmd.loadCartridge -18 @16:21:26.72 Gate.power :main,:ON,limit to 5000mA @16:21:26.75 Gate.power :analytic1,:ON,limit to 5000mA @16:21:26.77 Gate.power :analytic2,:ON,limit to 5000mA @16:21:26.79 Gate.power :analytic3,:ON,limit to 5000mA @16:21:26.92 Powering core bus @16:21:29.21 SPR Module 1, FW Rev. B, FPGA Rev. C @16:21:29.67 Archive.configure :default @16:21:29.80 Air.configure :default @16:21:29.90 Lysis1.configure :default @16:21:30.01 Lysis2.configure :default @16:21:30.13 Plunge.configure :default @16:21:30.25 SamplePump.configure :default @16:21:30.40 ExtraPump.configure :default @16:21:30.52 Toroid.configure :default @16:21:30.64 Carousel.configure :default @16:21:30.78 Pressure.configure :default @16:21:30.88 PinPort.configure :default @16:21:30.94 All elf controllers running firmware version 4.741 @16:21:30.97 Gate.power :motor,:ON,limit to 5000mA @16:21:31.16 Archive.in :default @16:21:31.43 Archive.home.jog 0 @16:21:31.60 PinPort.in :default @16:21:31.64 Toroid.in :default @16:21:31.68 Lysis2.in :default @16:21:31.71 Air.in :default @16:21:31.76 Lysis1.in :default @16:21:31.82 Plunge.in :default @16:21:32.02 PinPort.home.jog 0 @16:21:32.05 Toroid.home.jog 0 @16:21:32.07 Lysis2.home.jog 0 @16:21:32.11 Air.home.jog 0 @16:21:32.14 Lysis1.home.jog 0 @16:21:32.16 Plunge.home.jog 0 @16:21:32.67 Archive.in :homing @16:21:32.89 Toroid.in :homing @16:21:32.92 Plunge.in :homing @16:21:32.95 Lysis1.in :homing @16:21:32.99 Air.in :homing @16:21:33.03 Archive.jog 159 @16:21:33.07 PinPort.in :homing @16:21:33.10 Lysis2.in :homing @16:21:33.30 Toroid.jog 620 @16:21:33.35 Lysis1.jog 1413 @16:21:33.38 Air.jog 1405 @16:21:33.40 Plunge.jog 1569 @16:21:33.43 PinPort.jog -109 @16:21:33.47 Lysis2.jog 1411 @16:21:33.69 Archive.setRawPosition! 50 @16:21:33.74 Archive.seek :home @16:21:33.82 PinPort.setRawPosition! 0 @16:21:33.87 PinPort.seek :home @16:21:33.95 PinPort.in :default @16:21:34.04 Archive.in :default @16:21:34.69 Lysis1.setRawPosition! 50 @16:21:34.74 Lysis1.seek :home @16:21:34.85 Plunge.setRawPosition! 50 @16:21:34.87 Lysis2.setRawPosition! 50 @16:21:34.90 Air.setRawPosition! 50 @16:21:34.99 Plunge.seek :home @16:21:35.07 Lysis2.seek :home @16:21:35.12 Air.seek :home @16:21:35.24 Lysis1.in :default @16:21:35.32 Plunge.in :default @16:21:35.41 Lysis2.in :default @16:21:35.50 Air.in :default @16:21:35.58 Toroid.setRawPosition! 0 @16:21:35.63 Toroid.seek :home @16:21:35.73 Toroid.in :default @16:21:35.88 Toroid.seek :clear @16:21:37.50 Carousel.in :default @16:21:37.56 Carousel.home.jog 0 @16:21:38.05 Carousel.in :default @16:21:38.12 Carousel.seek 28 @16:21:38.27 Selecting Cartridge 25 @16:21:38.52 Carousel.seek 25 @16:21:39.40 Lysis1.in :fast @16:21:39.47 Lysis1.seek :locked @16:21:42.06 Plunge.seek :locked @16:21:42.18 Air.in :fast @16:21:42.24 Air.seek :locked @16:21:44.85 Powering cartridge bus @16:21:47.48 Gate.setIR 1750,5000,0,6 @16:21:47.61 Gate.setIR 0,5000,0,6 @16:21:47.67 PuckHeater.configure :default @16:21:47.73 Gate.power :heat,:ON,limit to 5000mA @16:21:48.40 Mounted lysis Cartridge board#Q05062 @16:21:48.42 Using default puck heat loss curve for HeatingAccelInCarousel @16:22:06.74 -> Cmd.startFiltering @16:22:07.20 Priming sample loop w/100ml, bypass w/10ml @16:22:07.21 SamplePump.setPosition! 0ml @16:22:07.25 Bypass.open @16:22:08.74 Intake.open @16:22:09.54 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:22:12.12 Vacuum tare is 51% of full scale @16:22:12.25 Pressure.in :default @16:22:12.31 Pressure.hold @16:22:12.49 SamplePump.in :prime @16:22:12.55 SamplePump.seek 10ml,59 seconds @16:22:21.81 Pressure.stop @16:22:21.86 SamplePump.setPosition! 0ml @16:22:21.91 Exhaust.open @16:22:23.32 Bypass.close @16:22:24.84 Pressure.in :default @16:22:24.90 Pressure.hold @16:22:25.12 SamplePump.in :prime @16:22:25.18 SamplePump.seek 100ml,5:16 @16:24:30.66 Pressure.stop @16:24:30.72 Bypass.open @16:24:31.43 Intake.close @16:24:32.28 Toroid.in :hold @16:24:32.34 Toroid.seek :crack @16:24:33.85 Toroid.seek :cartridge @16:24:34.93 Toroid.in :default @16:24:34.99 Toroid.seek :clear @16:24:36.95 Pressurizing cartridge to 14psi above 12.5psia @16:24:36.96 SamplePump.setPosition! 0ml @16:24:37.40 Intake.open @16:24:37.61 Bypass.close @16:24:38.34 Exhaust.close @16:24:39.16 Pressure.in :default @16:24:39.22 Pressure.hold @16:24:39.39 SamplePump.in :charge @16:24:39.46 SamplePump.seek 2.3ml,41 seconds @16:24:48.89 Pressure.stop @16:24:48.94 Intake.close @16:24:49.49 Pressurized cartridge with 0.396ml to 14.3psi @16:24:49.50 Isolated @24.5psia (11.9psi) @16:24:49.51 Verifying cartridge holds at least 0.75..0.85 of 24.5psia @16:24:49.54 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:24:54.17 Sampling 75.0ml @16:24:54.19 SamplePump.setPosition! 0ml @16:24:54.24 Exhaust.open @16:24:54.97 Intake.open @16:24:55.82 Pressure.in :default @16:24:55.89 Pressure.hold @16:24:56.11 SamplePump.in :sample @16:24:56.18 SamplePump.seek 75ml,5:31 @16:24:58.86 Cartridge 25 filtering 1/75ml at 0.32ml/s with 14.9psi vacuum @16:25:58.86 Cartridge 25 filtering 20/75ml at 0.34ml/s with 12.2psi vacuum @16:26:58.87 Cartridge 25 filtering 40/75ml at 0.31ml/s with 13.0psi vacuum @16:27:58.87 Cartridge 25 filtering 58/75ml at 0.30ml/s with 13.5psi vacuum @16:28:54.23 Pressure.stop @16:28:54.34 Intake.close @16:28:54.38 Exhaust.close @16:28:54.54 Toroid.in :hold @16:28:54.60 Toroid.seek :bypass @16:28:56.21 Toroid.in :default @16:28:56.27 Toroid.seek :clear @16:28:57.89 Sampled 75.0ml @16:28:59.23 -> Cmd.startProcessing @16:29:00.82 SPR.command \"read_sequences\" @16:29:01.20 SPR.command \"stats_on\" @16:29:01.24 SPR.command \"start 0 1\" @16:29:02.05 SPR.start /var/log/esp/spr21aug1116h.spr.bz2 @16:29:04.55 Handoff.select :waste @16:29:11.24 Syringe tare is 19% of full scale @16:29:11.26 PinPort.in :locking @16:29:11.34 PinPort.seek :locked @16:29:11.81 Archive.in :fast @16:29:11.86 Lysis2.in :fast @16:29:11.92 Lysis1.seek 1350uL @16:29:11.98 Archive.seek :full @16:29:12.05 Lysis2.seek :full @16:29:14.20 Plunge.seek :reagent @16:29:15.12 Evacuating puck with filter bubble point of 24psi @16:29:15.13 PinPort.seek :locked @16:29:15.22 Handoff.select :waste @16:29:21.81 Air syringe pumping up to 1 stroke to reach 8psi @16:29:21.82 Air stroke #1 of 1 @16:29:21.91 Air.in :pressurize @16:29:21.97 Air.seek :pressurized @16:29:33.22 0.2psi after pressurize @16:29:33.24 Air.in :compress @16:29:33.31 Air.seek :evac @16:29:37.40 0.8psi after compress @16:29:49.97 -0.0psi after compress (stable) @16:29:49.98 Air.in :default @16:29:50.05 Air.hold @16:29:50.23 at -0.0psi Opened to waste @16:29:50.25 Handoff.select :blocked @16:29:50.38 Archive syringe pumping up to 8 strokes to reach 24psi @16:29:50.39 Archive stroke #1 of 8 @16:29:50.47 Archive.in :pressurize @16:29:50.53 Archive.seek :pressurized @16:30:03.27 9.7psi after pressurize @16:30:03.29 Archive.in :compress @16:30:03.36 Archive.seek :empty @16:30:17.60 Reached 24.1 of 24.0psi after pumping 1695uL (0.98 strokes) @16:30:17.61 Archive.in :default @16:30:17.69 Archive.hold @16:30:17.75 Handoff.select :waste @16:30:27.94 delay 1:00 @16:31:27.97 Handoff.select :blocked @16:31:50.65 Handoff.select :waste @16:31:50.77 0.5psi at evacEnd @16:31:50.78 Handoff.select :blocked @16:31:50.91 Archive.in :fast @16:31:50.98 Archive.seek :topVent @16:32:04.59 Stable @0.6psi after Handoff blocked @16:32:04.61 Archive syringe pumping up to 8 strokes to reach 16psi @16:32:04.63 Archive stroke #1 of 8 @16:32:04.72 Archive.in :pressurize @16:32:04.78 Archive.seek :pressurized @16:32:17.54 0.6psi after pressurize @16:32:17.56 Archive.in :compress @16:32:17.63 Archive.seek :empty @16:32:33.51 14.4psi after compress @16:32:46.10 15.3psi after compress (stable) @16:32:46.11 Archive.in :gulp @16:32:46.19 Archive.seek :topVent @16:32:53.39 15.5psi after gulp @16:33:05.97 15.4psi after gulp (stable) @16:33:05.98 Archive stroke #2 of 8 @16:33:06.07 Archive.in :pressurize @16:33:06.13 Archive.seek :pressurized @16:33:18.86 15.4psi after pressurize @16:33:18.88 Archive.in :compress @16:33:18.96 Archive.seek :empty @16:33:19.15 Reached 15.3 of 15.6psi after pumping 3124uL (1.81 strokes) @16:33:19.16 Archive.in :default @16:33:19.24 Archive.hold @16:33:19.31 Handoff.select :waste @16:33:26.50 delay 1:00 @16:34:26.54 Handoff.select :blocked @16:34:49.22 Handoff.select :waste @16:34:49.34 0.7psi at evacEnd @16:34:49.36 Air.seek :fullVent @16:34:50.80 Archive.in :fast @16:34:50.86 Archive.seek :topVent @16:34:56.83 Seawater evacuated, Presure above/below filter released. Lysis begins @16:34:56.92 Lysis1.in :slow @16:34:56.98 Lysis1.seek 1050uL @16:35:27.02 delay 5 seconds @16:35:32.04 Handoff.select :blocked @16:35:32.21 Lysis1.seek 450uL @16:36:32.16 delay 3 seconds @16:36:35.23 Air.in to mix into 1.37806 parts Lysis1 @16:36:35.75 Air.seek :evac @16:36:35.79 Lysis1.seek 300uL @16:36:50.89 Lysis1.seek :empty @16:37:20.98 Lysis buffer delivered @19.4psi @16:37:20.99 Polling Can every 2:00 @16:37:21.54 PuckHeater.configure :default #ambient 25.0C @16:37:21.59 PuckHeater.seek 79.6C @16:38:10.10 PuckHeater.hold 2:00,79.6C @16:40:09.99 Polling Can every 2:00 @16:40:10.54 PuckHeater.configure :default #ambient 25.1C @16:40:10.59 PuckHeater.hold 30 seconds,78.6C @16:40:40.73 Polling Can every 2:00 @16:40:41.28 PuckHeater.configure :default #ambient 25.1C @16:40:41.33 PuckHeater.hold 30 seconds,78.1C @16:41:11.48 Polling Can every 2:00 @16:41:12.03 PuckHeater.configure :default #ambient 25.1C @16:41:12.08 PuckHeater.hold 10:00,77.6C @16:51:11.38 Lysis complete. Syringe pressure @17.9psi @16:51:11.39 Handoff.select :blocked @16:51:11.43 Archive syringe pumping up to 5 strokes to reach 40psi @16:51:11.45 Archive stroke #1 of 5 @16:51:11.56 Archive.in :pressurize @16:51:11.64 Archive.seek :pressurized @16:51:24.37 18.0psi after pressurize @16:51:24.39 Archive.in :compress @16:51:24.46 Archive.seek :empty @16:51:32.31 Reached 40.4 of 40.0psi after pumping 1559uL (0.90 strokes) @16:51:32.32 Archive.in :default @16:51:32.41 Archive.hold @16:51:32.48 delay 2 seconds @16:51:34.50 Archive.in :fast @16:51:34.56 Archive.seek :full @16:51:41.17 Lysis1.seek 138uL @16:51:55.03 delay 1 second @16:51:56.07 Archive.seek :full @16:51:56.09 Lysis1.seek :empty @16:52:10.06 Initial air pressure @49.1psi @16:52:10.12 Lysis1.in :extract @16:52:10.60 Lysis1.seek 600uL,nil @16:52:15.14 Archive syringe pumping up to 6 strokes to reach 25psi @16:52:15.15 Archive stroke #1 of 6 @16:52:15.29 Archive.in :pressurize @16:52:15.35 Archive.seek :pressurized @16:52:28.11 14.2psi after pressurize @16:52:28.12 Archive.in :compress @16:52:28.22 Archive.seek :empty @16:52:35.66 Syringe::LysatePlungerStuck in PROCESSING -- at < 410uL during Lysis Syringe #1 (LyS1) move @16:52:35.69 Lysis1.in :default @16:52:35.78 Lysis1.seek 375uL @16:52:36.83 Reached 26.0 of 25.0psi after pumping 1574uL (0.91 strokes) @16:52:36.84 Archive.in :default @16:52:36.91 Archive.hold @16:52:37.70 Lysis1.in :extract @16:52:37.78 Lysis1.seek 600uL,nil @16:52:41.97 Initial 600uL lysate @19.5psi @16:52:41.98 Handoff.select :blocked @16:52:42.08 Initial air pressure @20.4psi @16:52:42.15 Lysis1.seek 825uL,nil @16:52:45.08 Archive syringe pumping up to 6 strokes to reach 17psi @16:52:45.09 Archive stroke #1 of 6 @16:52:45.20 Archive.in :compress @16:52:45.29 Archive.seek :empty @16:52:48.13 Archive.in :default @16:52:48.18 Archive.hold @16:52:48.47 Recovered 825uL lysate @16.8psi @16:52:48.48 Air.in :halfSpeed @16:52:48.56 Air.seek :fullVent @16:52:50.80 Puck Top Vented, bottom @10.4psi @16:52:50.81 Handoff.select :waste @16:52:50.94 delay 5 seconds @16:52:56.01 Handoff open to waste, bottom @0.0psi @16:52:56.02 Air.in :default @16:52:56.09 Air.seek :evac @16:52:57.53 Lysis2.in :DAmix @16:52:57.64 Lysis1.in :DAmix @16:52:58.19 Lysis2.seek 1480uL @16:52:58.23 Lysis1.seek 575uL @16:53:33.27 delay 5 seconds @16:53:38.29 Handoff.select SPR @16:53:38.42 SPR.command \"start 2 14\" @16:53:39.58 SPR stabilized @16:53:39.71 delay 10 seconds @16:53:49.73 SPR.command \"start 2 0\" @16:53:53.44 Waiting up to 11:00 for SPR to enter state 1 @16:55:51.55 SPR.command \"hold_on\" @16:55:52.94 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @16:55:53.38 Lysis2.seek 1180uL @16:55:53.42 Lysis1.seek 275uL @16:56:35.43 delay 15 seconds @16:56:50.45 SPR.command \"hold_off\" @16:56:53.60 Waiting up to 38:00 for SPR to enter state 7 @17:20:50.75 SPR analysis completed @17:20:54.63 Ended log spr21aug1116h.spr.bz2 @17:20:54.64 SPR.command \"stats_off\" @17:20:56.27 -> Cmd.stop @17:20:56.29 Archive.stop @17:20:56.42 Handoff.select :close @17:20:56.55 Gate.power :heat,:OFF @17:20:56.57 Gate.power :cartridge,:OFF @17:20:56.65 Carousel.seek 25 @17:20:56.83 Plunge.seek :locked @17:20:57.71 PinPort.in :unlocking @17:20:57.77 PinPort.seek :clear @17:20:58.32 Archive.in :unlocking @17:20:58.44 Archive.seek :clear @17:20:58.50 Air.in :unlocking @17:20:58.63 Air.seek :clear @17:20:58.74 Lysis1.in :unlocking @17:20:58.82 Lysis1.seek :clear @17:20:58.89 Lysis2.in :unlocking @17:20:58.96 Lysis2.seek :clear @17:21:02.53 Gate.power :motor,:OFF @17:21:02.55 Gate.power :core,:OFF @17:21:02.57 Gate.power :analytic1,:OFF @17:21:02.59 Gate.power :analytic2,:OFF @17:21:02.61 Gate.power :analytic3,:OFF @17:21:02.64 Gate.power :main,:OFF @17:21:02.66 Safely stopped and ready to power off ##-- file closed 2021-08-12T01:00:41.602Z