##-- file opened 2023-04-15T02:24:18.401Z
@19:24:19.01 -> showStatus
@19:24:20.63 -> Cmd.loadCartridge -10
@19:24:20.70 Gate.power :main,:ON,limit to 5000mA
@19:24:20.72 Gate.power :analytic1,:ON,limit to 5000mA
@19:24:20.74 Gate.power :analytic2,:ON,limit to 5000mA
@19:24:20.77 Gate.power :analytic3,:ON,limit to 5000mA
@19:24:20.90 Powering core bus
@19:24:23.18 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@19:24:23.60 <LRAUV> Archive.configure :default
@19:24:23.69 Air.configure :default
@19:24:23.76 Lysis1.configure :default
@19:24:24.15 Lysis2.configure :default
@19:24:24.27 Plunge.configure :default
@19:24:24.36 SamplePump.configure :default
@19:24:24.45 Toroid.configure :default
@19:24:24.55 Carousel.configure :default
@19:24:24.64 Pressure.configure :default
@19:24:24.73 ExtraPump.configure :default
@19:24:24.80 PinPort.configure :default
@19:24:24.87 All elf controllers running firmware version 4.83
@19:24:24.90 Gate.power :motor,:ON,limit to 5000mA
@19:24:25.13 <Archive_home> Archive.home.jog 0
@19:24:25.62 <Carousel_home> Carousel.home.jog 0
@19:24:25.66 <Toroid_home> Toroid.home.jog 0
@19:24:25.70 <Lysis1_home> Lysis1.home.jog 0
@19:24:25.74 <Lysis2_home> Lysis2.home.jog 0
@19:24:25.79 <PinPort_home> PinPort.home.jog 0
@19:24:25.81 <Plunge_home> Plunge.home.jog 0
@19:24:25.84 <Air_home> Air.home.jog 0
@19:24:26.37 <Archive_home> Archive.in :homing
@19:24:26.51 Archive.jog 1382
@19:24:26.69 <Plunge_home> Plunge.in :homing
@19:24:26.72 <PinPort_home> PinPort.in :homing
@19:24:26.75 <Toroid_home> Toroid.in :homing
@19:24:26.80 <Lysis2_home> Lysis2.in :homing
@19:24:26.84 <Lysis1_home> Lysis1.in :homing
@19:24:26.88 <Air_home> Air.in :homing
@19:24:27.05 <Plunge_home> Plunge.jog 1536
@19:24:27.10 <PinPort_home> PinPort.jog -106
@19:24:27.20 <Toroid_home> Toroid.jog 620
@19:24:27.23 <Lysis1_home> Lysis1.jog 1394
@19:24:27.26 <Air_home> Air.jog 1375
@19:24:27.31 <Lysis2_home> Lysis2.jog 1395
@19:24:27.54 <PinPort_home> PinPort.setRawPosition! 0
@19:24:27.58 PinPort.seek :home
@19:24:27.67 <Archive_home> Archive.setRawPosition! 50
@19:24:27.70 <PinPort_home> PinPort.in :default
@19:24:27.76 <Archive_home> Archive.seek :home
@19:24:28.07 Archive.in :default
@19:24:28.39 <Plunge_home> Plunge.setRawPosition! 50
@19:24:28.44 Plunge.seek :home
@19:24:28.55 <Lysis1_home> Lysis1.setRawPosition! 50
@19:24:28.61 Lysis1.seek :home
@19:24:28.69 <Lysis2_home> Lysis2.setRawPosition! 50
@19:24:28.73 <Air_home> Air.setRawPosition! 50
@19:24:28.81 <Lysis2_home> Lysis2.seek :home
@19:24:28.84 <Air_home> Air.seek :home
@19:24:28.99 <Plunge_home> Plunge.in :default
@19:24:29.01 <Lysis1_home> Lysis1.in :default
@19:24:29.14 <Lysis2_home> Lysis2.in :default
@19:24:29.23 <Air_home> Air.in :default
@19:24:29.45 <Toroid_home> Toroid.setRawPosition! 0
@19:24:29.50 Toroid.seek :home
@19:24:29.58 Toroid.in :default
@19:24:29.66 <LRAUV> Toroid.seek :clear
@19:24:31.28 Carousel.in :default
@19:24:31.39 Carousel.home.seek 52
@19:24:31.71 Selecting Cartridge 52
@19:24:31.96 Carousel.seek 52
@19:24:32.15 Lysis1.in :fast
@19:24:32.21 Lysis1.seek :locked
@19:24:34.83 <Plunge> Plunge.seek :locked
@19:24:34.97 <Air> Air.in :fast
@19:24:35.04 Air.seek :locked
@19:24:36.62 <LRAUV> Carousel.reconfigure :locked
@19:24:36.77 Powering cartridge bus
@19:24:39.38 Gate.setIR 1750,5000,0,6
@19:24:39.50 Gate.setIR 0,5000,0,6
@19:24:39.57 PuckHeater.configure :default
@19:24:39.63 Gate.power :heat,:ON,limit to 5000mA
@19:24:42.42 Mounted lysis Cartridge board#Q05049
@19:24:42.48 Using default cartridge heat loss curve for HeatingAccelInCarousel
@19:24:58.20 -> Cmd.startFiltering
@19:24:58.47 <FILTERING> Preparing to filter cartridge 52
@19:24:58.75 Priming sample loop w/100ml, bypass w/10ml
@19:24:58.76 SamplePump.setPosition! 0ml
@19:24:58.83 Bypass.open
@19:25:00.26 Intake.open
@19:25:00.98 Sea 8.2m deep (26.8psia)
@19:25:01.00 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@19:25:03.58 Vacuum tare is 60% of full scale
@19:25:03.71 Pressure.in :default
@19:25:03.78 Pressure.hold
@19:25:03.97 SamplePump.in :prime
@19:25:04.04 SamplePump.seek 10ml,59 seconds
@19:25:12.78 Pressure.stop
@19:25:12.83 SamplePump.setPosition! 0ml
@19:25:12.89 Exhaust.open
@19:25:14.38 <closeBypass> Bypass.close
@19:25:15.85 <FILTERING> Pressure.in :default
@19:25:15.92 Pressure.hold
@19:25:16.05 Sea 7.7m deep (26.1psia)
@19:25:16.20 SamplePump.in :prime
@19:25:16.27 SamplePump.seek 100ml,5:16
@19:26:50.12 Pressure.stop
@19:26:50.20 Bypass.open
@19:26:50.85 Intake.close
@19:26:51.63 Toroid.in :hold
@19:26:51.69 Toroid.seek :crack
@19:26:53.23 Toroid.seek :cartridge
@19:26:54.33 Toroid.in :default
@19:26:54.39 Toroid.seek :clear
@19:26:56.75 Pressurizing cartridge to 26psi above 26.4psia
@19:26:56.79 SamplePump.setPosition! 0ml
@19:26:56.84 Intake.open
@19:26:57.50 Bypass.close
@19:26:58.17 Exhaust.close
@19:26:58.98 Pressure.in :default
@19:26:59.05 Pressure.hold
@19:26:59.24 SamplePump.in :charge
@19:26:59.31 SamplePump.seek 2.3ml,42 seconds
@19:27:08.87 Pressure.stop
@19:27:08.93 Intake.close
@19:27:09.66 Pressurized cartridge with 0.432ml to 26.4psi
@19:27:09.68 Isolated @49.3psia (22.9psi)
@19:27:09.70 Verifying cartridge holds at least 0.65..0.85 of 49.3psia
@19:27:09.73 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@19:27:14.33 Cartridge pressure rose 1% to 49.6psia
@19:27:14.42 Sampling 1000.0ml in 1:25:20
@19:27:14.43 SamplePump.setPosition! 0ml
@19:27:14.50 Exhaust.open
@19:27:15.23 Intake.open
@19:27:16.12 Pressure.in :default
@19:27:16.19 Pressure.hold
@19:27:16.31 Sea 9.2m deep (28.3psia)
@19:27:16.32 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s
@19:27:16.48 SamplePump.in :sample
@19:27:16.55 SamplePump.seek 1000ml,1:23:51
@19:27:19.15 <sampling> Cartridge 52 filtering 1/1000ml at 0.38ml/s with 23.4psi vacuum 7.4m deep (25.6psia)
@19:28:19.15 Cartridge 52 filtering 23/1000ml at 0.36ml/s with 25.6psi vacuum 6.0m deep (23.6psia)
@19:29:19.16 Cartridge 52 filtering 45/1000ml at 0.35ml/s with 25.6psi vacuum 6.3m deep (24.0psia)
@19:30:19.15 Cartridge 52 filtering 66/1000ml at 0.33ml/s with 24.6psi vacuum 6.0m deep (23.5psia)
@19:31:19.16 Cartridge 52 filtering 85/1000ml at 0.33ml/s with 24.0psi vacuum 8.3m deep (27.0psia)
@19:32:19.15 Cartridge 52 filtering 104/1000ml at 0.31ml/s with 22.6psi vacuum 8.5m deep (27.2psia)
@19:33:19.16 Cartridge 52 filtering 122/1000ml at 0.29ml/s with 23.7psi vacuum 7.9m deep (26.4psia)
@19:34:19.15 Cartridge 52 filtering 139/1000ml at 0.27ml/s with 23.5psi vacuum 7.4m deep (25.7psia)
@19:35:19.16 Cartridge 52 filtering 155/1000ml at 0.27ml/s with 24.1psi vacuum 6.6m deep (24.5psia)
@19:36:19.15 Cartridge 52 filtering 171/1000ml at 0.24ml/s with 23.3psi vacuum 6.5m deep (24.3psia)
@19:37:19.15 Cartridge 52 filtering 186/1000ml at 0.24ml/s with 23.5psi vacuum 7.0m deep (25.0psia)
@19:38:19.15 Cartridge 52 filtering 201/1000ml at 0.25ml/s with 25.8psi vacuum 7.2m deep (25.3psia)
@19:39:19.16 Cartridge 52 filtering 215/1000ml at 0.23ml/s with 23.9psi vacuum 6.6m deep (24.5psia)
@19:40:19.15 Cartridge 52 filtering 229/1000ml at 0.22ml/s with 23.6psi vacuum 7.1m deep (25.2psia)
@19:41:19.16 Cartridge 52 filtering 243/1000ml at 0.23ml/s with 23.8psi vacuum 6.2m deep (23.8psia)
@19:42:19.16 Cartridge 52 filtering 256/1000ml at 0.23ml/s with 23.7psi vacuum 7.5m deep (25.7psia)
@19:43:19.16 Cartridge 52 filtering 268/1000ml at 0.21ml/s with 25.2psi vacuum 7.1m deep (25.2psia)
@19:44:19.15 Cartridge 52 filtering 281/1000ml at 0.21ml/s with 24.2psi vacuum 6.5m deep (24.3psia)
@19:45:19.16 Cartridge 52 filtering 293/1000ml at 0.20ml/s with 24.2psi vacuum 6.1m deep (23.7psia)
@19:46:19.15 Cartridge 52 filtering 305/1000ml at 0.20ml/s with 22.8psi vacuum 8.7m deep (27.6psia)
@19:47:14.23 <FILTERING> Flow averaged 0.26ml/s over 19:58
@19:47:14.27 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi
@19:47:15.10 Relieved excess vacuum in .9 seconds
@19:47:15.13 SamplePump.seek 1000ml,57:29
@19:47:17.14 Flow estimate reduced to 0.21ml/s
@19:47:17.18 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi
@19:47:18.01 Relieved excess vacuum in .9 seconds
@19:47:18.04 SamplePump.seek 1000ml,57:27
@19:47:19.14 <sampling> Cartridge 52 filtering 317/1000ml at 0.32ml/s with 28.9psi vacuum 6.3m deep (24.0psia)
@19:47:20.95 <FILTERING> Flow estimate reduced to 0.17ml/s
@19:47:20.97 <closeIntake> Intake.close
@19:47:21.02 <closeExhaust> Exhaust.close
@19:47:21.27 <FILTERING> Toroid.in :hold
@19:47:21.35 Toroid.seek :bypass
@19:47:23.00 Toroid.in :default
@19:47:23.08 Toroid.seek :clear
@19:47:24.69 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@19:47:24.71 Pressure.stop
@19:47:24.90 Sampled  317.3ml
@19:47:26.78 <LRAUV> -> Cmd.startProcessing
@19:47:28.22 <PROCESSING> SPR.command \"read_sequences\"
@19:47:28.69 SPR.command \"stats_on\"
@19:47:28.81 SPR.command \"start 0 1\"
@19:47:29.54 <SPRlogger> SPR.start /var/log/esp/spr23apr1419h.spr.bz2
@19:47:32.02 <PROCESSING> Handoff.select :waste
@19:47:38.70 Syringe tare is 15% of full scale
@19:47:38.73 PinPort.in :locking
@19:47:38.80 PinPort.seek :locked
@19:47:39.71 <posArchive> Archive.in :fast
@19:47:39.75 <posLyS2> Lysis2.in :fast
@19:47:39.83 <PROCESSING> Lysis1.seek 1350uL
@19:47:39.90 <posArchive> Archive.seek :full
@19:47:40.00 <posLyS2> Lysis2.seek :full
@19:47:41.99 <PROCESSING> Plunge.seek :reagent
@19:47:42.88 Evacuating puck with filter bubble point of 50psi
@19:47:42.90 PinPort.seek :locked
@19:47:42.99 Handoff.select :waste
@19:47:51.08 Air syringe pumping up to 1 strokes to reach 8psi
@19:47:51.09 Air stroke #1 of 1
@19:47:51.19 Air.in :pressurize
@19:47:51.25 Air.seek :pressurized
@19:47:56.99 0.1psi after pressurize
@19:47:57.00 Air.in :compress
@19:47:57.09 Air.seek :evac
@19:48:00.09 0.5psi after compress
@19:48:10.67 -0.1psi after compress (stable)
@19:48:10.68 Air.in :default
@19:48:10.78 Air.hold
@19:48:10.98 at -0.1psi Opened to waste
@19:48:11.00 Handoff.select :blocked
@19:48:11.14 Archive syringe pumping up to 8 strokes to reach 50psi
@19:48:11.15 Archive stroke #1 of 8
@19:48:11.23 Archive.in :pressurize
@19:48:11.30 Archive.seek :pressurized
@19:48:23.80 -0.0psi after pressurize
@19:48:47.38 10.2psi after pressurize (stable)
@19:48:47.40 Archive.in :compress
@19:48:47.48 Archive.seek :empty
@19:49:02.10 10.5psi after compress
@19:49:12.67 10.4psi after compress (stable)
@19:49:12.69 Archive.in :gulp
@19:49:12.77 Archive.seek :topVent
@19:49:19.02 10.6psi after gulp
@19:49:24.61 10.6psi after gulp (stable)
@19:49:24.64 Archive stroke #2 of 8
@19:49:24.73 Archive.in :pressurize
@19:49:24.80 Archive.seek :pressurized
@19:49:37.30 10.5psi after pressurize
@19:49:42.87 10.4psi after pressurize (stable)
@19:49:42.89 Archive.in :compress
@19:49:42.96 Archive.seek :empty
@19:49:57.59 11.2psi after compress
@19:50:38.16 23.6psi after compress (stable)
@19:50:38.18 Archive.in :gulp
@19:50:38.26 Archive.seek :topVent
@19:50:44.54 24.4psi after gulp
@19:50:50.11 24.6psi after gulp (stable)
@19:50:50.12 Archive stroke #3 of 8
@19:50:50.21 Archive.in :pressurize
@19:50:50.29 Archive.seek :pressurized
@19:51:02.78 24.7psi after pressurize
@19:51:08.35 24.8psi after pressurize (stable)
@19:51:08.37 Archive.in :compress
@19:51:08.45 Archive.seek :empty
@19:51:23.08 29.3psi after compress
@19:51:55.66 44.5psi after compress (stable)
@19:51:55.67 Archive.in :gulp
@19:51:55.75 Archive.seek :topVent
@19:52:01.99 45.2psi after gulp
@19:52:07.57 45.5psi after gulp (stable)
@19:52:07.58 Archive stroke #4 of 8
@19:52:07.73 Archive.in :pressurize
@19:52:07.80 Archive.seek :pressurized
@19:52:20.30 45.6psi after pressurize
@19:52:25.87 45.6psi after pressurize (stable)
@19:52:25.89 Archive.in :compress
@19:52:25.97 Archive.seek :empty
@19:52:40.59 49.2psi after compress
@19:52:41.55 Reached 50.1 of 50.0psi after pumping 6920uL (4.00 strokes)
@19:52:41.56 Archive.in :default
@19:52:41.64 Archive.hold
@19:52:41.71 Handoff.select :waste
@19:52:55.40 delay 1:00
@19:53:55.41 Handoff.select :blocked
@19:54:18.10 Handoff.select :waste
@19:54:18.22 0.6psi at evacEnd
@19:54:18.23 Handoff.select :blocked
@19:54:18.37 Archive.in :fast
@19:54:18.44 Archive.seek :topVent
@19:54:32.11 Stable @0.7psi after Handoff blocked
@19:54:32.14 Archive syringe pumping up to 8 strokes to reach 31psi
@19:54:32.15 Archive stroke #1 of 8
@19:54:32.25 Archive.in :pressurize
@19:54:32.33 Archive.seek :pressurized
@19:54:44.82 0.7psi after pressurize
@19:54:44.83 Archive.in :compress
@19:54:44.92 Archive.seek :empty
@19:54:59.54 14.5psi after compress
@19:55:10.11 15.3psi after compress (stable)
@19:55:10.13 Archive.in :gulp
@19:55:10.22 Archive.seek :topVent
@19:55:16.49 15.2psi after gulp
@19:55:22.09 15.2psi after gulp (stable)
@19:55:22.10 Archive stroke #2 of 8
@19:55:22.21 Archive.in :pressurize
@19:55:22.28 Archive.seek :pressurized
@19:55:34.78 15.2psi after pressurize
@19:55:34.80 Archive.in :compress
@19:55:34.88 Archive.seek :empty
@19:55:49.52 28.8psi after compress
@19:56:00.12 29.6psi after compress (stable)
@19:56:00.13 Archive.in :gulp
@19:56:00.22 Archive.seek :topVent
@19:56:06.47 29.6psi after gulp
@19:56:12.05 29.6psi after gulp (stable)
@19:56:12.06 Archive stroke #3 of 8
@19:56:12.15 Archive.in :pressurize
@19:56:12.26 Archive.seek :pressurized
@19:56:24.75 29.5psi after pressurize
@19:56:24.77 Archive.in :compress
@19:56:24.85 Archive.seek :empty
@19:56:30.83 Reached 30.9 of 30.7psi after pumping 5003uL (2.89 strokes)
@19:56:30.85 Archive.in :default
@19:56:30.92 Archive.hold
@19:56:31.00 Handoff.select :waste
@19:56:38.20 delay 1:00
@19:57:38.21 Handoff.select :blocked
@19:58:00.92 Handoff.select :waste
@19:58:01.06 0.8psi at evacEnd
@19:58:01.08 Air.seek :fullVent
@19:58:02.50 Archive.in :fast
@19:58:02.57 Archive.seek :topVent
@19:58:09.03 Seawater evacuated, Pressure above/below filter released.
@19:58:09.11 Lysis1.in :slow
@19:58:09.18 Lysis1.seek 1050uL
@19:58:39.23 delay 5 seconds
@19:58:44.24 Handoff.select :blocked
@19:58:44.43 Lysis1.seek 450uL
@19:59:44.46 delay 3 seconds
@19:59:47.53 Air.in mix with 1.40048 parts Lysis1
@19:59:48.05 <Airmixer> Air.seek :evac
@19:59:48.09 <PROCESSING> Lysis1.seek 300uL
@20:00:03.22 Lysis1.seek :empty
@20:00:33.35 Lysis buffer delivered @21.1psi
@20:00:33.36 Polling Can every 2:00
@20:00:33.90 PuckHeater.configure :default #ambient 13.1C
@20:00:33.96 PuckHeater.seek 80.1C
@20:01:41.01 PuckHeater.hold 2:00,80.1C
@20:03:40.47 Polling Can every 2:00
@20:03:41.01 PuckHeater.configure :default #ambient 13.1C
@20:03:41.07 PuckHeater.hold 30 seconds,79.1C
@20:04:11.11 Polling Can every 2:00
@20:04:11.65 PuckHeater.configure :default #ambient 13.1C
@20:04:11.71 PuckHeater.hold 30 seconds,78.6C
@20:04:41.79 Polling Can every 2:00
@20:04:42.33 PuckHeater.configure :default #ambient 13.1C
@20:04:42.39 PuckHeater.hold 10:00,78.1C
@20:14:39.44 Lysis complete. Syringe pressure @19.9psi
@20:14:39.47 Archive syringe pumping up to 5 strokes to reach 40psi
@20:14:39.48 Archive stroke #1 of 5
@20:14:39.58 Archive.in :pressurize
@20:14:39.66 Archive.seek :pressurized
@20:14:52.15 19.6psi after pressurize
@20:14:57.75 19.4psi after pressurize (stable)
@20:14:57.77 Archive.in :compress
@20:14:57.87 Archive.seek :empty
@20:15:03.92 Reached 40.4 of 40.0psi after pumping 1545uL (0.89 strokes)
@20:15:03.94 Archive.in :default
@20:15:04.01 Archive.hold
@20:15:04.08 delay 2 seconds
@20:15:06.10 Archive.in :fast
@20:15:06.17 Archive.seek :full
@20:15:12.69 Lysis1.seek 138uL
@20:15:26.57 delay 1 second
@20:15:27.59 <fillArchive> Archive.seek :full
@20:15:27.63 <PROCESSING> Lysis1.seek :empty
@20:15:41.61 Handoff.select :blocked
@20:15:41.71 Initial air pressure @39.1psi
@20:15:41.78 Lysis1.in :extract
@20:15:41.87 Lysis1.seek 600uL,nil
@20:15:44.72 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@20:15:44.74 Archive stroke #1 of 6
@20:15:44.83 Archive.in :pressurize
@20:15:44.92 Archive.seek :pressurized
@20:15:57.44 13.1psi after pressurize
@20:16:03.42 Archive.in :default
@20:16:03.49 Archive.hold
@20:16:03.61 <PROCESSING> Initial 600uL lysate @14.7psi
@20:16:03.62 Handoff.select :blocked
@20:16:03.72 Initial air pressure @15.1psi
@20:16:03.80 Lysis1.seek 825uL,nil
@20:16:03.88 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@20:16:03.90 Archive stroke #1 of 6
@20:16:04.07 Archive.in :compress
@20:16:04.14 Archive.seek :empty
@20:16:18.82 10.9psi after compress
@20:16:29.40 11.2psi after compress (stable)
@20:16:34.56 Archive.in :gulp
@20:16:34.62 Archive.seek :topVent
@20:16:40.96 11.8psi after gulp
@20:16:46.55 11.9psi after gulp (stable)
@20:16:46.58 Archive stroke #2 of 6
@20:16:46.72 Archive.in :pressurize
@20:16:46.79 Archive.seek :pressurized
@20:16:59.28 12.3psi after pressurize
@20:17:04.90 12.7psi after pressurize (stable)
@20:17:04.91 Archive.in :compress
@20:17:05.00 Archive.seek :empty
@20:17:18.75 Archive.in :default
@20:17:18.82 Archive.hold
@20:17:19.07 <PROCESSING> Recovered 825uL lysate @15.8psi
@20:17:19.11 Air.in :halfSpeed
@20:17:19.18 Air.seek :fullVent
@20:17:21.38 Puck Top Vented, bottom @22.1psi
@20:17:21.40 Handoff.select :waste
@20:17:21.53 delay 5 seconds
@20:17:26.60 Handoff open to waste, bottom @0.4psi
@20:17:26.61 Air.in :default
@20:17:26.69 Air.seek :evac
@20:17:28.13 Lysis2.in :DAmix
@20:17:28.27 Lysis1.in :DAmix
@20:17:28.66 <Lysis2mixer> Lysis2.seek 1480uL
@20:17:28.70 <PROCESSING> Lysis1.seek 575uL
@20:18:03.81 delay 5 seconds
@20:18:08.83 Handoff.select SPR
@20:18:08.96 SPR.command \"start 2 14\"
@20:18:10.23 SPR stabilized
@20:18:10.37 delay 10 seconds
@20:18:20.39 SPR.command \"start 1 0\"
@20:18:23.59 Waiting up to 4:00 for SPR to enter state 1
@20:20:27.86 SPR.command \"hold_on\"
@20:20:29.27 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@20:20:29.73 <Lysis2mixer> Lysis2.seek 1180uL
@20:20:29.80 <PROCESSING> Lysis1.seek 275uL
@20:21:11.82 delay 15 seconds
@20:21:26.83 SPR.command \"hold_off\"
@20:21:29.93 Waiting up to 38:00 for SPR to enter state 7
@20:39:29.77 <SPRlogger> SPRsummary:-0.199RIU,164RIU,157RIU,238RIU,none,none,1.85ng/L after 3296s
@20:46:06.73 <PROCESSING> SPR analysis completed
@20:46:13.33 <SPRlogger> Ended log spr23apr1419h.spr.bz2
@20:46:13.35 <PROCESSING> SPR.command \"stats_off\"
@20:46:15.00 <LRAUV> -> Cmd.stop
@20:46:15.10 <closeHandoff> Handoff.select :close
@20:46:15.23 <LRAUV> Gate.power :heat,:OFF
@20:46:15.24 Gate.power :cartridge,:OFF
@20:46:15.33 PinPort.in :unlocking
@20:46:15.41 PinPort.seek :clear
@20:46:15.88 Carousel.in :default
@20:46:16.00 Carousel.home.seek 52
@20:46:16.30 Plunge.seek :locked
@20:46:17.17 <Archive> Archive.in :unlocking
@20:46:17.30 Archive.seek :clear
@20:46:17.36 <Air> Air.in :unlocking
@20:46:17.58 <Lysis1> Lysis1.in :unlocking
@20:46:17.63 <Air> Air.seek :clear
@20:46:17.81 <Lysis2> Lysis2.in :unlocking
@20:46:17.85 <Lysis1> Lysis1.seek :clear
@20:46:17.96 <Lysis2> Lysis2.seek :clear
@20:46:21.91 <LRAUV> Gate.power :motor,:OFF
@20:46:21.92 Gate.power :core,:OFF
@20:46:21.95 Gate.power :analytic1,:OFF
@20:46:21.98 Gate.power :analytic2,:OFF
@20:46:22.00 Gate.power :analytic3,:OFF
@20:46:22.02 Gate.power :main,:OFF
@20:46:22.04 Safely stopped and ready to power off
##-- file closed 2023-04-15T04:26:16.271Z

##-- file opened 2023-04-15T04:26:16.281Z
@21:26:16.10 -> showStatus
@21:26:18.12 -> Cmd.loadCartridge -6
@21:26:18.19 Gate.power :main,:ON,limit to 5000mA
@21:26:18.21 Gate.power :analytic1,:ON,limit to 5000mA
@21:26:18.23 Gate.power :analytic2,:ON,limit to 5000mA
@21:26:18.26 Gate.power :analytic3,:ON,limit to 5000mA
@21:26:18.39 Powering core bus
@21:26:20.66 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@21:26:21.09 <LRAUV> Archive.configure :default
@21:26:21.18 Air.configure :default
@21:26:21.61 Lysis1.configure :default
@21:26:21.68 Lysis2.configure :default
@21:26:21.77 Plunge.configure :default
@21:26:21.86 SamplePump.configure :default
@21:26:21.95 Toroid.configure :default
@21:26:22.04 Carousel.configure :default
@21:26:22.13 Pressure.configure :default
@21:26:22.22 ExtraPump.configure :default
@21:26:22.29 PinPort.configure :default
@21:26:22.36 All elf controllers running firmware version 4.83
@21:26:22.39 Gate.power :motor,:ON,limit to 5000mA
@21:26:22.76 <Archive_home> Archive.home.jog 0
@21:26:22.88 <Air_home> Air.home.jog 0
@21:26:22.97 <Lysis1_home> Lysis1.home.jog 0
@21:26:23.06 <Lysis2_home> Lysis2.home.jog 0
@21:26:23.15 <Plunge_home> Plunge.home.jog 0
@21:26:23.24 <Toroid_home> Toroid.home.jog 0
@21:26:23.31 <Carousel_home> Carousel.home.jog 0
@21:26:23.41 <PinPort_home> PinPort.home.jog 0
@21:26:24.06 <Plunge_home> Plunge.in :homing
@21:26:24.10 <Lysis1_home> Lysis1.in :homing
@21:26:24.14 <Lysis2_home> Lysis2.in :homing
@21:26:24.19 <Archive_home> Archive.in :homing
@21:26:24.27 <Air_home> Air.in :homing
@21:26:24.32 <Toroid_home> Toroid.in :homing
@21:26:24.50 <Plunge_home> Plunge.jog 1555
@21:26:24.55 <Lysis1_home> Lysis1.jog 155
@21:26:24.59 <Lysis2_home> Lysis2.jog 1406
@21:26:24.62 <Archive_home> Archive.jog 1390
@21:26:24.67 <Toroid_home> Toroid.jog 631
@21:26:24.71 <Air_home> Air.jog 1380
@21:26:24.96 <PinPort_home> PinPort.in :homing
@21:26:25.02 PinPort.jog -106
@21:26:25.17 <Lysis1_home> Lysis1.setRawPosition! 50
@21:26:25.23 Lysis1.seek :home
@21:26:25.29 <PinPort_home> PinPort.setRawPosition! 0
@21:26:25.37 PinPort.seek :home
@21:26:25.45 PinPort.in :default
@21:26:25.59 <Lysis1_home> Lysis1.in :default
@21:26:25.87 <Plunge_home> Plunge.setRawPosition! 50
@21:26:25.93 Plunge.seek :home
@21:26:26.04 <Air_home> Air.setRawPosition! 50
@21:26:26.06 <Lysis2_home> Lysis2.setRawPosition! 50
@21:26:26.11 <Archive_home> Archive.setRawPosition! 50
@21:26:26.17 <Air_home> Air.seek :home
@21:26:26.25 <Lysis2_home> Lysis2.seek :home
@21:26:26.35 <Archive_home> Archive.seek :home
@21:26:26.48 <Plunge_home> Plunge.in :default
@21:26:26.58 <Air_home> Air.in :default
@21:26:26.66 <Lysis2_home> Lysis2.in :default
@21:26:26.75 <Archive_home> Archive.in :default
@21:26:26.94 <Toroid_home> Toroid.setRawPosition! 0
@21:26:26.99 Toroid.seek :home
@21:26:27.07 Toroid.in :default
@21:26:27.16 <LRAUV> Toroid.seek :clear
@21:26:28.79 Carousel.in :default
@21:26:28.90 Carousel.home.seek 52
@21:26:29.16 Selecting Cartridge 44
@21:26:29.43 Carousel.seek 44
@21:26:31.70 Lysis1.in :fast
@21:26:31.78 Lysis1.seek :locked
@21:26:34.38 <Plunge> Plunge.seek :locked
@21:26:34.51 <Air> Air.in :fast
@21:26:34.59 Air.seek :locked
@21:26:36.15 <LRAUV> Carousel.reconfigure :locked
@21:26:36.31 Powering cartridge bus
@21:26:38.92 Gate.setIR 1750,5000,0,6
@21:26:39.04 Gate.setIR 0,5000,0,6
@21:26:39.10 Gate.power :cartridge,:OFF
@21:26:39.64 Mounted archive Cartridge
@21:26:54.88 -> Cmd.startFiltering
@21:26:55.13 <FILTERING> Preparing to filter cartridge 44
@21:26:55.41 Priming sample loop w/100ml, bypass w/10ml
@21:26:55.43 SamplePump.setPosition! 0ml
@21:26:55.48 Bypass.open
@21:26:56.93 Intake.open
@21:26:57.67 Sea 12.3m deep (32.8psia)
@21:26:57.69 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@21:27:00.28 Vacuum tare is 60% of full scale
@21:27:00.42 Pressure.in :default
@21:27:00.48 Pressure.hold
@21:27:00.67 SamplePump.in :prime
@21:27:00.75 SamplePump.seek 10ml,59 seconds
@21:27:09.49 Pressure.stop
@21:27:09.54 SamplePump.setPosition! 0ml
@21:27:09.59 Exhaust.open
@21:27:11.09 <closeBypass> Bypass.close
@21:27:12.57 <FILTERING> Pressure.in :default
@21:27:12.64 Pressure.hold
@21:27:12.76 Sea 12.5m deep (33.2psia)
@21:27:12.91 SamplePump.in :prime
@21:27:12.99 SamplePump.seek 100ml,5:16
@21:28:50.08 Pressure.stop
@21:28:50.16 Bypass.open
@21:28:50.84 Intake.close
@21:28:51.60 Toroid.in :hold
@21:28:51.67 Toroid.seek :crack
@21:28:53.20 Toroid.seek :cartridge
@21:28:54.31 Toroid.in :default
@21:28:54.38 Toroid.seek :clear
@21:28:56.35 Pressurizing cartridge to 26psi above 33.4psia
@21:28:56.36 SamplePump.setPosition! 0ml
@21:28:56.42 Intake.open
@21:28:57.47 Bypass.close
@21:28:58.15 Exhaust.close
@21:28:58.99 Pressure.in :default
@21:28:59.07 Pressure.hold
@21:28:59.25 SamplePump.in :charge
@21:28:59.33 SamplePump.seek 2.3ml,42 seconds
@21:29:03.57 Pressure.stop
@21:29:03.63 Intake.close
@21:29:04.41 Pressurized cartridge with 0.082ml to 26.2psi
@21:29:04.42 Isolated @53.6psia (20.2psi)
@21:29:04.45 Verifying cartridge holds at least 0.65..0.85 of 53.6psia
@21:29:04.48 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@21:29:09.07 Cartridge pressure fell 2% to 52.7psia
@21:29:09.16 Sampling 1000.0ml in 1:25:20
@21:29:09.18 SamplePump.setPosition! 0ml
@21:29:09.24 Exhaust.open
@21:29:09.99 Intake.open
@21:29:10.85 Pressure.in :default
@21:29:10.92 Pressure.hold
@21:29:11.04 Sea 12.1m deep (32.6psia)
@21:29:11.05 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s
@21:29:11.21 SamplePump.in :sample
@21:29:11.29 SamplePump.seek 1000ml,1:23:51
@21:29:13.89 <sampling> Cartridge 44 filtering 1/1000ml at 0.41ml/s with 25.8psi vacuum 10.1m deep (29.6psia)
@21:30:13.90 Cartridge 44 filtering 25/1000ml at 0.40ml/s with 24.9psi vacuum 10.6m deep (30.3psia)
@21:31:13.90 Cartridge 44 filtering 49/1000ml at 0.40ml/s with 25.6psi vacuum 9.2m deep (28.3psia)
@21:32:13.89 Cartridge 44 filtering 72/1000ml at 0.40ml/s with 25.5psi vacuum 12.5m deep (33.1psia)
@21:33:13.90 Cartridge 44 filtering 95/1000ml at 0.36ml/s with 26.3psi vacuum 11.7m deep (31.9psia)
@21:34:13.89 Cartridge 44 filtering 117/1000ml at 0.35ml/s with 25.8psi vacuum 8.9m deep (27.9psia)
@21:35:13.90 Cartridge 44 filtering 139/1000ml at 0.36ml/s with 19.9psi vacuum 12.3m deep (32.9psia)
@21:36:13.89 Cartridge 44 filtering 160/1000ml at 0.34ml/s with 21.5psi vacuum 14.0m deep (35.4psia)
@21:37:13.90 Cartridge 44 filtering 181/1000ml at 0.34ml/s with 26.5psi vacuum 9.7m deep (29.1psia)
@21:38:13.89 Cartridge 44 filtering 201/1000ml at 0.32ml/s with 24.1psi vacuum 11.6m deep (31.8psia)
@21:39:13.90 Cartridge 44 filtering 221/1000ml at 0.34ml/s with 19.9psi vacuum 14.4m deep (36.0psia)
@21:40:13.89 Cartridge 44 filtering 240/1000ml at 0.34ml/s with 24.2psi vacuum 11.3m deep (31.4psia)
@21:41:13.90 Cartridge 44 filtering 259/1000ml at 0.30ml/s with 22.2psi vacuum 12.0m deep (32.5psia)
@21:42:13.89 Cartridge 44 filtering 277/1000ml at 0.29ml/s with 27.2psi vacuum 8.5m deep (27.2psia)
@21:43:13.90 Cartridge 44 filtering 295/1000ml at 0.29ml/s with 24.5psi vacuum 10.7m deep (30.5psia)
@21:44:13.94 Cartridge 44 filtering 312/1000ml at 0.31ml/s with 23.6psi vacuum 12.1m deep (32.5psia)
@21:45:14.28 Cartridge 44 filtering 330/1000ml at 0.29ml/s with 22.5psi vacuum 13.1m deep (34.0psia)
@21:46:13.89 Cartridge 44 filtering 346/1000ml at 0.27ml/s with 26.9psi vacuum 10.8m deep (30.7psia)
@21:47:13.90 Cartridge 44 filtering 362/1000ml at 0.26ml/s with 21.8psi vacuum 11.5m deep (31.6psia)
@21:48:13.89 Cartridge 44 filtering 378/1000ml at 0.27ml/s with 22.2psi vacuum 12.7m deep (33.5psia)
@21:49:13.90 Cartridge 44 filtering 394/1000ml at 0.26ml/s with 24.7psi vacuum 10.8m deep (30.7psia)
@21:50:13.89 Cartridge 44 filtering 409/1000ml at 0.24ml/s with 22.1psi vacuum 11.1m deep (31.1psia)
@21:51:13.89 Cartridge 44 filtering 424/1000ml at 0.23ml/s with 22.1psi vacuum 12.6m deep (33.3psia)
@21:52:13.89 Cartridge 44 filtering 438/1000ml at 0.24ml/s with 26.4psi vacuum 11.5m deep (31.7psia)
@21:53:13.90 Cartridge 44 filtering 453/1000ml at 0.24ml/s with 23.8psi vacuum 11.6m deep (31.9psia)
@21:54:13.89 Cartridge 44 filtering 466/1000ml at 0.24ml/s with 24.5psi vacuum 10.9m deep (30.8psia)
@21:55:13.90 Cartridge 44 filtering 480/1000ml at 0.22ml/s with 22.6psi vacuum 9.2m deep (28.3psia)
@21:56:13.89 Cartridge 44 filtering 493/1000ml at 0.22ml/s with 23.6psi vacuum 12.0m deep (32.5psia)
@21:57:13.90 Cartridge 44 filtering 506/1000ml at 0.22ml/s with 24.0psi vacuum 10.1m deep (29.6psia)
@21:58:13.89 Cartridge 44 filtering 519/1000ml at 0.23ml/s with 22.5psi vacuum 12.9m deep (33.8psia)
@21:59:13.90 Cartridge 44 filtering 531/1000ml at 0.20ml/s with 25.0psi vacuum 9.7m deep (29.0psia)
@22:00:13.89 Cartridge 44 filtering 544/1000ml at 0.22ml/s with 25.9psi vacuum 10.3m deep (29.9psia)
@22:01:13.90 Cartridge 44 filtering 556/1000ml at 0.21ml/s with 25.3psi vacuum 11.3m deep (31.4psia)
@22:02:13.89 Cartridge 44 filtering 568/1000ml at 0.20ml/s with 25.9psi vacuum 9.9m deep (29.3psia)
@22:03:13.90 Cartridge 44 filtering 580/1000ml at 0.20ml/s with 26.8psi vacuum 11.5m deep (31.7psia)
@22:03:14.05 <FILTERING> Flow averaged 0.28ml/s over 34:03
@22:03:14.09 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi
@22:03:14.67 Relieved excess vacuum in .6 seconds
@22:03:14.70 SamplePump.seek 1000ml,35:28
@22:03:50.11 Flow averaged 0.20ml/s over 36 seconds
@22:03:50.15 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi
@22:03:50.73 Relieved excess vacuum in .6 seconds
@22:03:50.76 SamplePump.seek 1000ml,34:53
@22:04:13.89 <sampling> Cartridge 44 filtering 592/1000ml at 0.20ml/s with 26.6psi vacuum 10.8m deep (30.7psia)
@22:04:32.66 <FILTERING> Flow averaged 0.20ml/s over 42 seconds
@22:04:32.70 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@22:04:33.29 Relieved excess vacuum in .6 seconds
@22:04:33.32 SamplePump.seek 1000ml,34:10
@22:04:50.11 Flow averaged 0.20ml/s over 17 seconds
@22:04:50.13 <closeIntake> Intake.close
@22:04:50.18 <closeExhaust> Exhaust.close
@22:04:50.41 <FILTERING> Toroid.in :hold
@22:04:50.49 Toroid.seek :bypass
@22:04:52.13 Toroid.in :default
@22:04:52.21 Toroid.seek :clear
@22:04:53.82 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@22:04:53.84 Pressure.stop
@22:04:54.04 Sampled  599.4ml
@22:04:55.90 <LRAUV> -> Cmd.startProcessing
@22:04:56.76 <PROCESSING> Plunge.seek :reagent
@22:04:57.57 Sample loop closed. Ready to Deliver Reagents
@22:04:57.59 Archive.seek :sealed
@22:05:01.53 Archive.seek :evac
@22:05:15.58 delay 1:00
@22:06:15.61 Archive.seek :clear
@22:06:33.12 RNAlater soak for 10:00
@22:16:33.15 Archive.seek :tVent
@22:16:35.60 delay 1 second
@22:16:36.62 Archive.seek :evac
@22:16:52.14 delay 10 seconds
@22:17:02.17 Sample preserved and evacuated
@22:17:03.13 <LRAUV> -> Cmd.stop
@22:17:03.28 Carousel.in :default
@22:17:03.39 Carousel.home.seek 44
@22:17:03.69 Plunge.seek :locked
@22:17:04.57 <Archive> Archive.in :unlocking
@22:17:04.71 Archive.seek :clear
@22:17:04.75 <Air> Air.in :unlocking
@22:17:04.92 Air.seek :clear
@22:17:04.97 <Lysis1> Lysis1.in :unlocking
@22:17:05.13 Lysis1.seek :clear
@22:17:09.39 <LRAUV> Gate.power :motor,:OFF
@22:17:09.41 Gate.power :core,:OFF
@22:17:09.43 Gate.power :analytic1,:OFF
@22:17:09.45 Gate.power :analytic2,:OFF
@22:17:09.48 Gate.power :analytic3,:OFF
@22:17:09.50 Gate.power :main,:OFF
@22:17:09.52 Safely stopped and ready to power off
##-- file closed 2023-04-15T14:54:00.479Z

##-- file opened 2023-04-15T14:54:00.487Z
@07:54:00.80 -> showStatus
@07:54:02.82 -> Cmd.loadCartridge -10
@07:54:02.88 Gate.power :main,:ON,limit to 5000mA
@07:54:02.91 Gate.power :analytic1,:ON,limit to 5000mA
@07:54:02.93 Gate.power :analytic2,:ON,limit to 5000mA
@07:54:02.96 Gate.power :analytic3,:ON,limit to 5000mA
@07:54:03.10 Powering core bus
@07:54:05.36 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@07:54:05.80 <LRAUV> Archive.configure :default
@07:54:06.25 Air.configure :default
@07:54:06.32 Lysis1.configure :default
@07:54:06.43 Lysis2.configure :default
@07:54:06.52 Plunge.configure :default
@07:54:06.61 SamplePump.configure :default
@07:54:06.70 Toroid.configure :default
@07:54:06.79 Carousel.configure :default
@07:54:06.89 Pressure.configure :default
@07:54:06.98 ExtraPump.configure :default
@07:54:07.05 PinPort.configure :default
@07:54:07.11 All elf controllers running firmware version 4.83
@07:54:07.14 Gate.power :motor,:ON,limit to 5000mA
@07:54:07.36 <Archive_home> Archive.home.jog 0
@07:54:07.84 <Toroid_home> Toroid.home.jog 0
@07:54:07.88 <Plunge_home> Plunge.home.jog 0
@07:54:07.90 <Air_home> Air.home.jog 0
@07:54:07.94 <PinPort_home> PinPort.home.jog 0
@07:54:07.97 <Carousel_home> Carousel.home.jog 0
@07:54:08.00 <Lysis1_home> Lysis1.home.jog 0
@07:54:08.05 <Lysis2_home> Lysis2.home.jog 0
@07:54:08.66 <Archive_home> Archive.in :homing
@07:54:08.84 <Toroid_home> Toroid.in :homing
@07:54:08.91 <Archive_home> Archive.jog 1391
@07:54:08.95 <PinPort_home> PinPort.in :homing
@07:54:08.98 <Plunge_home> Plunge.in :homing
@07:54:09.02 <Lysis1_home> Lysis1.in :homing
@07:54:09.05 <Air_home> Air.in :homing
@07:54:09.10 <Lysis2_home> Lysis2.in :homing
@07:54:09.32 <Toroid_home> Toroid.jog 627
@07:54:09.35 <PinPort_home> PinPort.jog -106
@07:54:09.40 <Plunge_home> Plunge.jog 1550
@07:54:09.44 <Lysis1_home> Lysis1.jog 162
@07:54:09.50 <Air_home> Air.jog 1381
@07:54:09.57 <Lysis2_home> Lysis2.jog 1404
@07:54:09.78 <PinPort_home> PinPort.setRawPosition! 0
@07:54:09.82 PinPort.seek :home
@07:54:09.91 PinPort.in :default
@07:54:10.06 <Lysis1_home> Lysis1.setRawPosition! 50
@07:54:10.11 Lysis1.seek :home
@07:54:10.31 <Archive_home> Archive.setRawPosition! 50
@07:54:10.36 Archive.seek :home
@07:54:10.46 <Lysis1_home> Lysis1.in :default
@07:54:10.70 <Archive_home> Archive.in :default
@07:54:10.82 <Air_home> Air.setRawPosition! 50
@07:54:10.88 Air.seek :home
@07:54:10.96 <Plunge_home> Plunge.setRawPosition! 50
@07:54:10.98 <Lysis2_home> Lysis2.setRawPosition! 50
@07:54:11.06 <Plunge_home> Plunge.seek :home
@07:54:11.10 <Lysis2_home> Lysis2.seek :home
@07:54:11.23 <Air_home> Air.in :default
@07:54:11.40 <Plunge_home> Plunge.in :default
@07:54:11.49 <Lysis2_home> Lysis2.in :default
@07:54:11.59 <Toroid_home> Toroid.setRawPosition! 0
@07:54:11.65 Toroid.seek :home
@07:54:11.78 Toroid.in :default
@07:54:11.86 <LRAUV> Toroid.seek :clear
@07:54:13.48 Carousel.in :default
@07:54:13.60 Carousel.home.seek 44
@07:54:13.86 Selecting Cartridge 51
@07:54:14.13 Carousel.seek 51
@07:54:16.21 Lysis1.in :fast
@07:54:16.28 Lysis1.seek :locked
@07:54:18.88 <Plunge> Plunge.seek :locked
@07:54:19.02 <Air> Air.in :fast
@07:54:19.08 Air.seek :locked
@07:54:20.66 <LRAUV> Carousel.reconfigure :locked
@07:54:20.82 Powering cartridge bus
@07:54:23.44 Gate.setIR 1750,5000,0,6
@07:54:23.56 Gate.setIR 0,5000,0,6
@07:54:23.63 PuckHeater.configure :default
@07:54:23.69 Gate.power :heat,:ON,limit to 5000mA
@07:54:24.39 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05040
@07:54:24.43 Using default cartridge heat loss curve for HeatingAccelInCarousel
@07:54:40.42 -> Cmd.startFiltering
@07:54:40.66 <FILTERING> Preparing to filter cartridge 51
@07:54:40.94 Priming sample loop w/100ml, bypass w/10ml
@07:54:40.95 SamplePump.setPosition! 0ml
@07:54:41.00 Bypass.open
@07:54:42.46 Intake.open
@07:54:43.19 Sea 11.3m deep (31.4psia)
@07:54:43.20 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@07:54:45.80 Vacuum tare is 59% of full scale
@07:54:45.94 Pressure.in :default
@07:54:46.00 Pressure.hold
@07:54:46.19 SamplePump.in :prime
@07:54:46.26 SamplePump.seek 10ml,59 seconds
@07:54:55.01 Pressure.stop
@07:54:55.06 SamplePump.setPosition! 0ml
@07:54:55.12 Exhaust.open
@07:54:56.60 <closeBypass> Bypass.close
@07:54:58.10 <FILTERING> Pressure.in :default
@07:54:58.16 Pressure.hold
@07:54:58.28 Sea 11.3m deep (31.4psia)
@07:54:58.42 SamplePump.in :prime
@07:54:58.50 SamplePump.seek 100ml,5:16
@07:56:36.78 Pressure.stop
@07:56:36.86 Bypass.open
@07:56:37.56 Intake.close
@07:56:38.36 Toroid.in :hold
@07:56:38.43 Toroid.seek :crack
@07:56:39.98 Toroid.seek :cartridge
@07:56:41.09 Toroid.in :default
@07:56:41.16 Toroid.seek :clear
@07:56:43.52 Pressurizing cartridge to 26psi above 30.9psia
@07:56:43.54 SamplePump.setPosition! 0ml
@07:56:43.59 Intake.open
@07:56:44.31 Bypass.close
@07:56:44.99 Exhaust.close
@07:56:45.83 Pressure.in :default
@07:56:45.89 Pressure.hold
@07:56:46.08 SamplePump.in :charge
@07:56:46.17 SamplePump.seek 2.3ml,42 seconds
@07:56:55.17 Pressure.stop
@07:56:55.24 Intake.close
@07:56:55.99 Pressurized cartridge with 0.383ml to 26.8psi
@07:56:56.01 Isolated @53.1psia (22.2psi)
@07:56:56.03 Verifying cartridge holds at least 0.65..0.85 of 53.1psia
@07:56:56.06 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@07:57:01.66 Cartridge pressure fell 11% to 47.1psia
@07:57:01.75 Sampling 1000.0ml in 1:25:20
@07:57:01.77 SamplePump.setPosition! 0ml
@07:57:01.83 Exhaust.open
@07:57:02.60 Intake.open
@07:57:03.44 Pressure.in :default
@07:57:03.50 Pressure.hold
@07:57:03.63 Sea 11.1m deep (31.1psia)
@07:57:03.64 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s
@07:57:03.79 SamplePump.in :sample
@07:57:03.86 SamplePump.seek 1000ml,1:23:51
@07:57:06.47 <sampling> Cartridge 51 filtering 1/1000ml at 0.38ml/s with 23.8psi vacuum 11.0m deep (31.0psia)
@07:58:06.48 Cartridge 51 filtering 24/1000ml at 0.37ml/s with 24.1psi vacuum 10.0m deep (29.5psia)
@07:59:06.48 Cartridge 51 filtering 46/1000ml at 0.38ml/s with 23.0psi vacuum 11.3m deep (31.4psia)
@08:00:06.47 Cartridge 51 filtering 67/1000ml at 0.36ml/s with 22.9psi vacuum 8.6m deep (27.4psia)
@08:01:06.48 Cartridge 51 filtering 88/1000ml at 0.36ml/s with 23.6psi vacuum 10.2m deep (29.7psia)
@08:02:06.48 Cartridge 51 filtering 109/1000ml at 0.34ml/s with 26.2psi vacuum 8.7m deep (27.5psia)
@08:03:06.48 Cartridge 51 filtering 129/1000ml at 0.30ml/s with 23.9psi vacuum 8.0m deep (26.5psia)
@08:04:06.47 Cartridge 51 filtering 148/1000ml at 0.32ml/s with 27.2psi vacuum 8.3m deep (27.0psia)
@08:05:06.48 Cartridge 51 filtering 167/1000ml at 0.30ml/s with 22.9psi vacuum 9.5m deep (28.8psia)
@08:06:06.48 Cartridge 51 filtering 185/1000ml at 0.28ml/s with 23.7psi vacuum 7.7m deep (26.1psia)
@08:07:06.48 Cartridge 51 filtering 203/1000ml at 0.31ml/s with 22.9psi vacuum 8.2m deep (26.9psia)
@08:08:06.48 Cartridge 51 filtering 221/1000ml at 0.31ml/s with 25.7psi vacuum 9.8m deep (29.2psia)
@08:09:06.83 Cartridge 51 filtering 238/1000ml at 0.26ml/s with 26.0psi vacuum 8.0m deep (26.5psia)
@08:10:06.48 Cartridge 51 filtering 255/1000ml at 0.28ml/s with 23.7psi vacuum 7.7m deep (26.1psia)
@08:11:06.48 Cartridge 51 filtering 272/1000ml at 0.27ml/s with 24.2psi vacuum 9.9m deep (29.3psia)
@08:12:06.48 Cartridge 51 filtering 288/1000ml at 0.27ml/s with 26.4psi vacuum 6.9m deep (24.9psia)
@08:13:06.48 Cartridge 51 filtering 304/1000ml at 0.25ml/s with 23.2psi vacuum 8.9m deep (27.8psia)
@08:14:06.48 Cartridge 51 filtering 321/1000ml at 0.27ml/s with 25.6psi vacuum 8.2m deep (26.8psia)
@08:15:06.48 Cartridge 51 filtering 336/1000ml at 0.26ml/s with 25.9psi vacuum 7.9m deep (26.4psia)
@08:16:06.47 Cartridge 51 filtering 352/1000ml at 0.25ml/s with 22.2psi vacuum 8.5m deep (27.2psia)
@08:17:06.48 Cartridge 51 filtering 368/1000ml at 0.24ml/s with 22.7psi vacuum 9.4m deep (28.7psia)
@08:18:06.48 Cartridge 51 filtering 383/1000ml at 0.27ml/s with 21.3psi vacuum 9.1m deep (28.2psia)
@08:19:06.48 Cartridge 51 filtering 398/1000ml at 0.25ml/s with 21.7psi vacuum 9.5m deep (28.8psia)
@08:20:06.48 Cartridge 51 filtering 413/1000ml at 0.27ml/s with 24.2psi vacuum 7.6m deep (26.0psia)
@08:21:06.48 Cartridge 51 filtering 428/1000ml at 0.25ml/s with 21.9psi vacuum 8.0m deep (26.5psia)
@08:22:06.47 Cartridge 51 filtering 443/1000ml at 0.24ml/s with 22.4psi vacuum 8.7m deep (27.6psia)
@08:23:06.48 Cartridge 51 filtering 457/1000ml at 0.24ml/s with 25.1psi vacuum 9.5m deep (28.8psia)
@08:24:06.48 Cartridge 51 filtering 472/1000ml at 0.26ml/s with 23.6psi vacuum 8.1m deep (26.7psia)
@08:25:06.48 Cartridge 51 filtering 486/1000ml at 0.24ml/s with 25.0psi vacuum 7.8m deep (26.3psia)
@08:26:06.48 Cartridge 51 filtering 500/1000ml at 0.24ml/s with 21.3psi vacuum 8.6m deep (27.4psia)
@08:27:06.48 Cartridge 51 filtering 514/1000ml at 0.23ml/s with 26.6psi vacuum 7.1m deep (25.1psia)
@08:28:06.48 Cartridge 51 filtering 528/1000ml at 0.25ml/s with 23.7psi vacuum 7.1m deep (25.2psia)
@08:29:06.48 Cartridge 51 filtering 542/1000ml at 0.22ml/s with 26.2psi vacuum 8.3m deep (27.0psia)
@08:30:06.47 Cartridge 51 filtering 555/1000ml at 0.20ml/s with 23.8psi vacuum 7.6m deep (25.9psia)
@08:31:06.48 Cartridge 51 filtering 569/1000ml at 0.22ml/s with 25.3psi vacuum 6.6m deep (24.4psia)
@08:32:06.48 Cartridge 51 filtering 582/1000ml at 0.21ml/s with 23.6psi vacuum 7.8m deep (26.2psia)
@08:33:06.48 Cartridge 51 filtering 595/1000ml at 0.22ml/s with 21.7psi vacuum 8.7m deep (27.5psia)
@08:34:06.48 Cartridge 51 filtering 609/1000ml at 0.21ml/s with 24.8psi vacuum 7.5m deep (25.7psia)
@08:35:06.48 Cartridge 51 filtering 622/1000ml at 0.21ml/s with 22.2psi vacuum 7.8m deep (26.2psia)
@08:36:06.48 Cartridge 51 filtering 635/1000ml at 0.23ml/s with 22.7psi vacuum 8.6m deep (27.4psia)
@08:37:06.48 Cartridge 51 filtering 648/1000ml at 0.22ml/s with 23.9psi vacuum 7.0m deep (25.0psia)
@08:38:06.48 Cartridge 51 filtering 660/1000ml at 0.20ml/s with 24.6psi vacuum 7.7m deep (26.0psia)
@08:39:06.48 Cartridge 51 filtering 673/1000ml at 0.20ml/s with 22.1psi vacuum 8.9m deep (27.9psia)
@08:40:06.48 Cartridge 51 filtering 686/1000ml at 0.21ml/s with 24.2psi vacuum 8.3m deep (26.9psia)
@08:41:06.48 Cartridge 51 filtering 698/1000ml at 0.20ml/s with 24.4psi vacuum 7.1m deep (25.1psia)
@08:42:06.47 Cartridge 51 filtering 711/1000ml at 0.21ml/s with 26.8psi vacuum 7.3m deep (25.5psia)
@08:43:06.48 Cartridge 51 filtering 723/1000ml at 0.20ml/s with 25.4psi vacuum 7.4m deep (25.7psia)
@08:44:06.48 Cartridge 51 filtering 735/1000ml at 0.20ml/s with 25.6psi vacuum 8.1m deep (26.7psia)
@08:45:06.47 Cartridge 51 filtering 748/1000ml at 0.20ml/s with 25.2psi vacuum 7.4m deep (25.7psia)
@08:46:06.79 Cartridge 51 filtering 760/1000ml at 0.22ml/s with 23.7psi vacuum 9.1m deep (28.1psia)
@08:47:06.48 Cartridge 51 filtering 772/1000ml at 0.20ml/s with 24.8psi vacuum 7.1m deep (25.1psia)
@08:48:06.48 Cartridge 51 filtering 784/1000ml at 0.20ml/s with 23.6psi vacuum 8.4m deep (27.1psia)
@08:49:06.48 Cartridge 51 filtering 797/1000ml at 0.20ml/s with 24.7psi vacuum 8.0m deep (26.5psia)
@08:50:06.48 Cartridge 51 filtering 809/1000ml at 0.20ml/s with 25.3psi vacuum 8.3m deep (26.9psia)
@08:51:06.48 Cartridge 51 filtering 821/1000ml at 0.20ml/s with 26.5psi vacuum 7.1m deep (25.2psia)
@08:52:06.48 Cartridge 51 filtering 833/1000ml at 0.20ml/s with 25.8psi vacuum 8.4m deep (27.1psia)
@08:53:06.48 Cartridge 51 filtering 845/1000ml at 0.20ml/s with 25.9psi vacuum 6.9m deep (24.9psia)
@08:54:06.48 Cartridge 51 filtering 857/1000ml at 0.20ml/s with 27.3psi vacuum 7.1m deep (25.2psia)
@08:55:06.48 Cartridge 51 filtering 869/1000ml at 0.20ml/s with 25.8psi vacuum 6.6m deep (24.5psia)
@08:55:24.10 <FILTERING> Flow averaged 0.25ml/s over 58:21
@08:55:24.14 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@08:55:24.73 Relieved excess vacuum in .6 seconds
@08:55:24.75 SamplePump.seek 1000ml,11:07
@08:56:06.48 <sampling> Cartridge 51 filtering 881/1000ml at 0.20ml/s with 26.3psi vacuum 8.0m deep (26.5psia)
@08:57:06.48 Cartridge 51 filtering 893/1000ml at 0.20ml/s with 23.3psi vacuum 8.3m deep (27.0psia)
@08:57:20.63 <FILTERING> Flow averaged 0.20ml/s over 1:56
@08:57:20.66 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi
@08:57:21.25 Relieved excess vacuum in .6 seconds
@08:57:21.28 SamplePump.seek 1000ml,9:10
@08:58:06.47 <sampling> Cartridge 51 filtering 905/1000ml at 0.20ml/s with 25.9psi vacuum 7.3m deep (25.5psia)
@08:58:19.23 <FILTERING> Flow averaged 0.20ml/s over 58 seconds
@08:58:19.27 Waiting up to 8 seconds for vacuum to reduce from 26.0 to 18.0psi
@08:58:19.85 Relieved excess vacuum in .6 seconds
@08:58:19.88 SamplePump.seek 1000ml,8:12
@08:59:06.47 <sampling> Cartridge 51 filtering 917/1000ml at 0.20ml/s with 28.4psi vacuum 7.1m deep (25.2psia)
@08:59:43.61 <FILTERING> Flow averaged 0.20ml/s over 1:24
@08:59:43.64 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@08:59:44.23 Relieved excess vacuum in .6 seconds
@08:59:44.26 SamplePump.seek 1000ml,6:48
@08:59:46.31 Flow estimate reduced to 0.16ml/s
@08:59:46.33 <closeIntake> Intake.close
@08:59:46.40 <closeExhaust> Exhaust.close
@08:59:46.60 <FILTERING> Toroid.in :hold
@08:59:46.68 Toroid.seek :bypass
@08:59:48.32 Toroid.in :default
@08:59:48.40 Toroid.seek :clear
@08:59:50.02 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@08:59:50.04 Pressure.stop
@08:59:50.23 Sampled  924.9ml
@08:59:52.44 <LRAUV> -> Cmd.startProcessing
@08:59:53.87 <PROCESSING> SPR.command \"read_sequences\"
@08:59:54.08 SPR.command \"stats_on\"
@08:59:54.63 SPR.command \"start 0 1\"
@08:59:55.36 <SPRlogger> SPR.start /var/log/esp/spr23apr1508h.spr.bz2
@08:59:56.93 <PROCESSING> Handoff.select :waste
@09:00:03.61 Syringe tare is 16% of full scale
@09:00:03.63 PinPort.in :locking
@09:00:03.72 PinPort.seek :locked
@09:00:04.20 <posArchive> Archive.in :fast
@09:00:04.25 <posLyS2> Lysis2.in :fast
@09:00:04.29 <PROCESSING> Lysis1.seek 1350uL
@09:00:04.45 <posLyS2> Lysis2.seek :full
@09:00:04.52 <posArchive> Archive.seek :full
@09:00:06.44 <PROCESSING> Plunge.seek :reagent
@09:00:07.37 Evacuating puck with filter bubble point of 50psi
@09:00:07.39 PinPort.seek :locked
@09:00:07.48 Handoff.select :waste
@09:00:14.07 Air syringe pumping up to 1 strokes to reach 8psi
@09:00:14.08 Air stroke #1 of 1
@09:00:14.18 Air.in :pressurize
@09:00:14.24 Air.seek :pressurized
@09:00:19.98 1.0psi after pressurize
@09:00:19.99 Air.in :compress
@09:00:20.10 Air.seek :evac
@09:00:23.11 1.1psi after compress
@09:00:33.68 0.0psi after compress (stable)
@09:00:33.69 Air.in :default
@09:00:33.77 Air.hold
@09:00:33.95 at -0.1psi Opened to waste
@09:00:33.96 Handoff.select :blocked
@09:00:34.10 Archive syringe pumping up to 8 strokes to reach 50psi
@09:00:34.11 Archive stroke #1 of 8
@09:00:34.20 Archive.in :pressurize
@09:00:34.28 Archive.seek :pressurized
@09:00:46.79 0.7psi after pressurize
@09:01:10.36 11.3psi after pressurize (stable)
@09:01:10.38 Archive.in :compress
@09:01:10.46 Archive.seek :empty
@09:01:25.08 12.7psi after compress
@09:01:35.66 13.5psi after compress (stable)
@09:01:35.68 Archive.in :gulp
@09:01:35.77 Archive.seek :topVent
@09:01:42.04 14.2psi after gulp
@09:01:47.61 14.5psi after gulp (stable)
@09:01:47.62 Archive stroke #2 of 8
@09:01:47.72 Archive.in :pressurize
@09:01:47.79 Archive.seek :pressurized
@09:02:00.28 15.0psi after pressurize
@09:02:05.85 15.2psi after pressurize (stable)
@09:02:05.87 Archive.in :compress
@09:02:05.95 Archive.seek :empty
@09:02:20.57 16.4psi after compress
@09:02:31.14 17.2psi after compress (stable)
@09:02:31.15 Archive.in :gulp
@09:02:31.24 Archive.seek :topVent
@09:02:37.48 17.9psi after gulp
@09:02:43.05 18.2psi after gulp (stable)
@09:02:43.06 Archive stroke #3 of 8
@09:02:43.16 Archive.in :pressurize
@09:02:43.22 Archive.seek :pressurized
@09:02:55.72 18.4psi after pressurize
@09:03:01.29 18.5psi after pressurize (stable)
@09:03:01.31 Archive.in :compress
@09:03:01.40 Archive.seek :empty
@09:03:16.03 21.4psi after compress
@09:04:00.64 48.8psi after compress (stable)
@09:04:00.66 Archive.in :gulp
@09:04:00.74 Archive.seek :topVent
@09:04:01.16 Reached 50.2 of 50.0psi after pumping 5190uL (3.00 strokes)
@09:04:01.18 Archive.in :default
@09:04:01.26 Archive.hold
@09:04:01.33 Handoff.select :waste
@09:04:13.52 delay 1:00
@09:05:13.54 Handoff.select :blocked
@09:05:36.23 Handoff.select :waste
@09:05:36.35 0.1psi at evacEnd
@09:05:36.36 Handoff.select :blocked
@09:05:36.49 Archive.in :fast
@09:05:36.57 Archive.seek :topVent
@09:05:50.06 Stable @0.2psi after Handoff blocked
@09:05:50.08 Archive syringe pumping up to 8 strokes to reach 30psi
@09:05:50.10 Archive stroke #1 of 8
@09:05:50.22 Archive.in :pressurize
@09:05:50.29 Archive.seek :pressurized
@09:06:02.79 0.2psi after pressurize
@09:06:02.81 Archive.in :compress
@09:06:02.89 Archive.seek :empty
@09:06:17.50 1.4psi after compress
@09:06:47.07 10.3psi after compress (stable)
@09:06:47.09 Archive.in :gulp
@09:06:47.18 Archive.seek :topVent
@09:06:53.43 11.1psi after gulp
@09:06:59.01 11.4psi after gulp (stable)
@09:06:59.02 Archive stroke #2 of 8
@09:06:59.11 Archive.in :pressurize
@09:06:59.18 Archive.seek :pressurized
@09:07:11.70 12.0psi after pressurize
@09:07:11.71 Archive.in :compress
@09:07:11.80 Archive.seek :empty
@09:07:26.42 15.3psi after compress
@09:07:54.49 25.2psi after compress (stable)
@09:07:54.51 Archive.in :gulp
@09:07:54.60 Archive.seek :topVent
@09:08:00.84 26.0psi after gulp
@09:08:06.44 26.3psi after gulp (stable)
@09:08:06.46 Archive stroke #3 of 8
@09:08:06.56 Archive.in :pressurize
@09:08:06.62 Archive.seek :pressurized
@09:08:19.11 26.8psi after pressurize
@09:08:19.13 Archive.in :compress
@09:08:19.21 Archive.seek :empty
@09:08:33.86 29.4psi after compress
@09:08:34.88 Reached 30.3 of 30.2psi after pumping 5190uL (3.00 strokes)
@09:08:34.89 Archive.in :default
@09:08:34.97 Archive.hold
@09:08:35.04 Handoff.select :waste
@09:08:42.23 delay 1:00
@09:09:42.24 Handoff.select :blocked
@09:10:05.22 Handoff.select :waste
@09:10:05.34 0.2psi at evacEnd
@09:10:05.36 Air.seek :fullVent
@09:10:06.79 Archive.in :fast
@09:10:06.86 Archive.seek :topVent
@09:10:13.96 Seawater evacuated, Pressure above/below filter released.
@09:10:14.06 Lysis1.in :slow
@09:10:14.16 Lysis1.seek 1050uL
@09:10:44.18 delay 5 seconds
@09:10:49.20 Handoff.select :blocked
@09:10:49.38 Lysis1.seek 450uL
@09:11:49.36 delay 3 seconds
@09:11:52.45 Air.in mix with 1.40048 parts Lysis1
@09:11:53.19 <Airmixer> Air.seek :evac
@09:11:53.25 <PROCESSING> Lysis1.seek 300uL
@09:12:08.36 Lysis1.seek :empty
@09:12:38.46 Lysis buffer delivered @17.0psi
@09:12:38.47 Polling Can every 2:00
@09:12:39.03 PuckHeater.configure :default #ambient 11.4C
@09:12:39.08 PuckHeater.seek 80.2C
@09:13:52.15 PuckHeater.hold 2:00,80.2C
@09:15:52.20 Polling Can every 2:00
@09:15:52.74 PuckHeater.configure :default #ambient 11.5C
@09:15:52.80 PuckHeater.hold 30 seconds,79.2C
@09:16:22.98 Polling Can every 2:00
@09:16:23.53 PuckHeater.configure :default #ambient 11.5C
@09:16:23.59 PuckHeater.hold 30 seconds,78.7C
@09:16:53.77 Polling Can every 2:00
@09:16:54.32 PuckHeater.hold 10:00,78.1C
@09:26:53.90 Lysis complete. Syringe pressure @19.2psi
@09:26:53.92 Archive syringe pumping up to 5 strokes to reach 40psi
@09:26:53.93 Archive stroke #1 of 5
@09:26:54.04 Archive.in :pressurize
@09:26:54.10 Archive.seek :pressurized
@09:27:06.60 18.8psi after pressurize
@09:27:30.67 37.9psi after pressurize (stable)
@09:27:30.71 Archive.in :compress
@09:27:30.86 Archive.seek :empty
@09:27:34.63 Reached 40.1 of 40.0psi after pumping 1496uL (0.86 strokes)
@09:27:34.64 Archive.in :default
@09:27:34.72 Archive.hold
@09:27:34.79 delay 2 seconds
@09:27:36.81 Archive.in :fast
@09:27:36.87 Archive.seek :full
@09:27:43.23 Lysis1.seek 138uL
@09:27:57.11 delay 1 second
@09:27:58.15 <fillArchive> Archive.seek :full
@09:27:58.18 <PROCESSING> Lysis1.seek :empty
@09:28:12.12 Handoff.select :blocked
@09:28:12.28 Initial air pressure @41.9psi
@09:28:12.37 Lysis1.in :extract
@09:28:12.46 Lysis1.seek 600uL,nil
@09:28:14.51 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@09:28:14.52 Archive stroke #1 of 6
@09:28:14.62 Archive.in :pressurize
@09:28:14.69 Archive.seek :pressurized
@09:28:27.18 13.3psi after pressurize
@09:28:43.92 Archive.in :default
@09:28:43.97 Archive.hold
@09:28:44.14 <PROCESSING> Initial 600uL lysate @14.8psi
@09:28:44.15 Handoff.select :blocked
@09:28:44.26 Initial air pressure @14.9psi
@09:28:44.34 Lysis1.seek 825uL,nil
@09:28:44.42 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@09:28:44.43 Archive stroke #1 of 6
@09:28:44.60 Archive.in :compress
@09:28:44.67 Archive.seek :empty
@09:28:55.65 Archive.in :default
@09:28:55.70 Archive.hold
@09:28:55.96 <PROCESSING> Recovered 825uL lysate @15.6psi
@09:28:55.98 Air.in :halfSpeed
@09:28:56.06 Air.seek :fullVent
@09:28:58.26 Puck Top Vented, bottom @2.2psi
@09:28:58.27 Handoff.select :waste
@09:28:58.40 delay 5 seconds
@09:29:03.47 Handoff open to waste, bottom @0.0psi
@09:29:03.48 Air.in :default
@09:29:03.56 Air.seek :evac
@09:29:04.97 Lysis2.in :DAmix
@09:29:05.09 Lysis1.in :DAmix
@09:29:05.61 <Lysis2mixer> Lysis2.seek 1480uL
@09:29:05.65 <PROCESSING> Lysis1.seek 575uL
@09:29:40.76 delay 5 seconds
@09:29:45.78 Handoff.select SPR
@09:29:45.90 SPR.command \"start 2 14\"
@09:29:46.45 SPR stabilized
@09:29:46.59 delay 10 seconds
@09:29:56.61 SPR.command \"start 1 0\"
@09:29:59.81 Waiting up to 4:00 for SPR to enter state 1
@09:32:04.07 SPR.command \"hold_on\"
@09:32:05.49 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@09:32:05.96 <Lysis2mixer> Lysis2.seek 1180uL
@09:32:06.00 <PROCESSING> Lysis1.seek 275uL
@09:32:48.07 delay 15 seconds
@09:33:03.08 SPR.command \"hold_off\"
@09:33:06.13 Waiting up to 38:00 for SPR to enter state 7
@09:51:05.72 <SPRlogger> SPRsummary:-10.5RIU,135RIU,139RIU,195RIU,1.62ng/L,none,3.46ng/L after 3244s
@09:57:42.94 <PROCESSING> SPR analysis completed
@09:57:48.68 <SPRlogger> Ended log spr23apr1508h.spr.bz2
@09:57:48.70 <PROCESSING> SPR.command \"stats_off\"
@09:57:50.24 <LRAUV> -> Cmd.stop
@09:57:50.33 <closeHandoff> Handoff.select :close
@09:57:50.47 <LRAUV> Gate.power :heat,:OFF
@09:57:50.49 Gate.power :cartridge,:OFF
@09:57:50.58 PinPort.in :unlocking
@09:57:50.65 PinPort.seek :clear
@09:57:51.13 Carousel.in :default
@09:57:51.24 Carousel.home.seek 51
@09:57:51.54 Plunge.seek :locked
@09:57:52.41 <Archive> Archive.in :unlocking
@09:57:52.54 Archive.seek :clear
@09:57:52.61 <Air> Air.in :unlocking
@09:57:52.75 Air.seek :clear
@09:57:52.79 <Lysis1> Lysis1.in :unlocking
@09:57:52.97 Lysis1.seek :clear
@09:57:53.02 <Lysis2> Lysis2.in :unlocking
@09:57:53.12 Lysis2.seek :clear
@09:57:57.06 <LRAUV> Gate.power :motor,:OFF
@09:57:57.08 Gate.power :core,:OFF
@09:57:57.10 Gate.power :analytic1,:OFF
@09:57:57.12 Gate.power :analytic2,:OFF
@09:57:57.14 Gate.power :analytic3,:OFF
@09:57:57.17 Gate.power :main,:OFF
@09:57:57.19 Safely stopped and ready to power off
##-- file closed 2023-04-15T17:28:50.327Z

##-- file opened 2023-04-15T17:28:50.334Z
@10:28:50.15 -> showStatus
@10:28:52.17 -> Cmd.loadCartridge -6
@10:28:52.24 Gate.power :main,:ON,limit to 5000mA
@10:28:52.25 Gate.power :analytic1,:ON,limit to 5000mA
@10:28:52.28 Gate.power :analytic2,:ON,limit to 5000mA
@10:28:52.30 Gate.power :analytic3,:ON,limit to 5000mA
@10:28:52.44 Powering core bus
@10:28:54.71 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@10:28:55.49 <LRAUV> Archive.configure :default
@10:28:55.58 Air.configure :default
@10:28:55.65 Lysis1.configure :default
@10:28:55.72 Lysis2.configure :default
@10:28:55.80 Plunge.configure :default
@10:28:55.89 SamplePump.configure :default
@10:28:55.98 Toroid.configure :default
@10:28:56.07 Carousel.configure :default
@10:28:56.16 Pressure.configure :default
@10:28:56.25 ExtraPump.configure :default
@10:28:56.32 PinPort.configure :default
@10:28:56.39 All elf controllers running firmware version 4.83
@10:28:56.42 Gate.power :motor,:ON,limit to 5000mA
@10:28:56.76 <Lysis2_home> Lysis2.home.jog 0
@10:28:56.87 <Archive_home> Archive.home.jog 0
@10:28:57.00 <Lysis1_home> Lysis1.home.jog 0
@10:28:57.07 <Air_home> Air.home.jog 0
@10:28:57.43 <Carousel_home> Carousel.home.jog 0
@10:28:57.46 <Toroid_home> Toroid.home.jog 0
@10:28:57.53 <PinPort_home> PinPort.home.jog 0
@10:28:57.59 <Plunge_home> Plunge.home.jog 0
@10:28:58.02 <Lysis1_home> Lysis1.in :homing
@10:28:58.10 <Air_home> Air.in :homing
@10:28:58.14 <Archive_home> Archive.in :homing
@10:28:58.18 <Lysis2_home> Lysis2.in :homing
@10:28:58.31 <Lysis1_home> Lysis1.jog 162
@10:28:58.39 <Air_home> Air.jog 1388
@10:28:58.44 <Lysis2_home> Lysis2.jog 1407
@10:28:58.51 <Archive_home> Archive.jog 1393
@10:28:58.69 <Plunge_home> Plunge.in :homing
@10:28:58.72 <Toroid_home> Toroid.in :homing
@10:28:58.76 <PinPort_home> PinPort.in :homing
@10:28:58.89 <Plunge_home> Plunge.jog 1549
@10:28:58.94 <Toroid_home> Toroid.jog 633
@10:28:58.98 <PinPort_home> PinPort.jog -106
@10:28:59.12 <Lysis1_home> Lysis1.setRawPosition! 50
@10:28:59.22 Lysis1.seek :home
@10:28:59.30 <PinPort_home> PinPort.setRawPosition! 0
@10:28:59.36 PinPort.seek :home
@10:28:59.43 PinPort.in :default
@10:28:59.52 <Lysis1_home> Lysis1.in :default
@10:28:59.65 <Air_home> Air.setRawPosition! 50
@10:28:59.70 Air.seek :home
@10:28:59.80 <Lysis2_home> Lysis2.setRawPosition! 50
@10:28:59.82 <Archive_home> Archive.setRawPosition! 50
@10:28:59.90 <Lysis2_home> Lysis2.seek :home
@10:28:59.96 <Archive_home> Archive.seek :home
@10:29:00.08 <Air_home> Air.in :default
@10:29:00.20 <Plunge_home> Plunge.setRawPosition! 50
@10:29:00.22 <Lysis2_home> Lysis2.in :default
@10:29:00.33 <Plunge_home> Plunge.seek :home
@10:29:00.43 <Archive_home> Archive.in :default
@10:29:00.62 <Plunge_home> Plunge.in :default
@10:29:01.16 <Toroid_home> Toroid.setRawPosition! 0
@10:29:01.21 Toroid.seek :home
@10:29:01.29 Toroid.in :default
@10:29:01.36 <LRAUV> Toroid.seek :clear
@10:29:02.99 Carousel.in :default
@10:29:03.10 Carousel.home.seek 51
@10:29:03.36 Selecting Cartridge 42
@10:29:03.63 Carousel.seek 42
@10:29:06.10 Lysis1.in :fast
@10:29:06.16 Lysis1.seek :locked
@10:29:08.77 <Plunge> Plunge.seek :locked
@10:29:08.91 <Air> Air.in :fast
@10:29:08.99 Air.seek :locked
@10:29:10.56 <LRAUV> Carousel.reconfigure :locked
@10:29:10.70 Powering cartridge bus
@10:29:13.31 Gate.setIR 1750,5000,0,6
@10:29:13.44 Gate.setIR 0,5000,0,6
@10:29:13.50 Gate.power :cartridge,:OFF
@10:29:14.03 Mounted archive Cartridge
@10:29:28.93 -> Cmd.startFiltering
@10:29:29.17 <FILTERING> Preparing to filter cartridge 42
@10:29:29.45 Priming sample loop w/100ml, bypass w/10ml
@10:29:29.47 SamplePump.setPosition! 0ml
@10:29:29.52 Bypass.open
@10:29:31.01 Intake.open
@10:29:31.71 Sea 12.1m deep (32.6psia)
@10:29:31.74 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@10:29:34.31 Vacuum tare is 60% of full scale
@10:29:34.45 Pressure.in :default
@10:29:34.51 Pressure.hold
@10:29:34.70 SamplePump.in :prime
@10:29:34.77 SamplePump.seek 10ml,59 seconds
@10:29:43.53 Pressure.stop
@10:29:43.59 SamplePump.setPosition! 0ml
@10:29:43.64 Exhaust.open
@10:29:45.08 <closeBypass> Bypass.close
@10:29:46.60 <FILTERING> Pressure.in :default
@10:29:46.67 Pressure.hold
@10:29:46.78 Sea 11.8m deep (32.2psia)
@10:29:46.93 SamplePump.in :prime
@10:29:47.00 SamplePump.seek 100ml,5:16
@10:31:24.54 Pressure.stop
@10:31:24.61 Bypass.open
@10:31:25.30 Intake.close
@10:31:26.05 Toroid.in :hold
@10:31:26.12 Toroid.seek :crack
@10:31:27.64 Toroid.seek :cartridge
@10:31:28.78 Toroid.in :default
@10:31:28.85 Toroid.seek :clear
@10:31:30.81 Pressurizing cartridge to 26psi above 32.3psia
@10:31:30.82 SamplePump.setPosition! 0ml
@10:31:31.28 Intake.open
@10:31:31.95 Bypass.close
@10:31:32.61 Exhaust.close
@10:31:33.45 Pressure.in :default
@10:31:33.52 Pressure.hold
@10:31:33.70 SamplePump.in :charge
@10:31:33.77 SamplePump.seek 2.3ml,42 seconds
@10:31:38.11 Pressure.stop
@10:31:38.18 Intake.close
@10:31:39.06 Pressurized cartridge with 0.086ml to 26.2psi
@10:31:39.08 Isolated @54.9psia (22.6psi)
@10:31:39.10 Verifying cartridge holds at least 0.65..0.85 of 54.9psia
@10:31:39.13 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@10:31:43.72 Cartridge pressure fell 3% to 53.5psia
@10:31:43.81 Sampling 1000.0ml in 1:25:20
@10:31:43.83 SamplePump.setPosition! 0ml
@10:31:43.91 Exhaust.open
@10:31:44.67 Intake.open
@10:31:45.52 Pressure.in :default
@10:31:45.59 Pressure.hold
@10:31:45.71 Sea 11.7m deep (32.0psia)
@10:31:45.72 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s
@10:31:45.87 SamplePump.in :sample
@10:31:45.95 SamplePump.seek 1000ml,1:23:51
@10:31:48.55 <sampling> Cartridge 42 filtering 1/1000ml at 0.39ml/s with 26.8psi vacuum 7.8m deep (26.3psia)
@10:32:48.55 Cartridge 42 filtering 25/1000ml at 0.39ml/s with 24.3psi vacuum 11.7m deep (32.0psia)
@10:33:48.56 Cartridge 42 filtering 49/1000ml at 0.39ml/s with 23.3psi vacuum 11.7m deep (32.0psia)
@10:34:48.55 Cartridge 42 filtering 72/1000ml at 0.37ml/s with 23.7psi vacuum 11.0m deep (30.9psia)
@10:35:48.56 Cartridge 42 filtering 95/1000ml at 0.37ml/s with 24.9psi vacuum 10.7m deep (30.4psia)
@10:36:48.55 Cartridge 42 filtering 118/1000ml at 0.36ml/s with 24.4psi vacuum 10.3m deep (30.0psia)
@10:37:48.55 Cartridge 42 filtering 140/1000ml at 0.37ml/s with 25.9psi vacuum 10.7m deep (30.5psia)
@10:38:48.55 Cartridge 42 filtering 162/1000ml at 0.37ml/s with 27.6psi vacuum 9.4m deep (28.6psia)
@10:39:48.56 Cartridge 42 filtering 184/1000ml at 0.35ml/s with 24.7psi vacuum 10.0m deep (29.4psia)
@10:40:48.55 Cartridge 42 filtering 205/1000ml at 0.33ml/s with 21.6psi vacuum 12.8m deep (33.5psia)
@10:41:48.56 Cartridge 42 filtering 226/1000ml at 0.36ml/s with 25.7psi vacuum 11.7m deep (31.9psia)
@10:42:48.60 Cartridge 42 filtering 247/1000ml at 0.34ml/s with 25.8psi vacuum 8.6m deep (27.4psia)
@10:43:48.56 Cartridge 42 filtering 267/1000ml at 0.32ml/s with 26.2psi vacuum 7.7m deep (26.0psia)
@10:44:48.55 Cartridge 42 filtering 287/1000ml at 0.33ml/s with 21.9psi vacuum 12.3m deep (32.9psia)
@10:45:48.56 Cartridge 42 filtering 307/1000ml at 0.34ml/s with 23.7psi vacuum 13.6m deep (34.8psia)
@10:46:48.92 Cartridge 42 filtering 327/1000ml at 0.35ml/s with 24.6psi vacuum 10.2m deep (29.8psia)
@10:47:48.56 Cartridge 42 filtering 346/1000ml at 0.33ml/s with 27.7psi vacuum 8.1m deep (26.7psia)
@10:48:48.56 Cartridge 42 filtering 366/1000ml at 0.30ml/s with 21.3psi vacuum 12.7m deep (33.5psia)
@10:49:48.56 Cartridge 42 filtering 385/1000ml at 0.32ml/s with 23.7psi vacuum 13.0m deep (33.9psia)
@10:50:48.55 Cartridge 42 filtering 403/1000ml at 0.32ml/s with 23.7psi vacuum 12.4m deep (33.1psia)
@10:51:48.56 Cartridge 42 filtering 422/1000ml at 0.30ml/s with 24.5psi vacuum 10.4m deep (30.1psia)
@10:52:48.56 Cartridge 42 filtering 440/1000ml at 0.30ml/s with 26.3psi vacuum 9.9m deep (29.3psia)
@10:53:48.56 Cartridge 42 filtering 458/1000ml at 0.29ml/s with 26.9psi vacuum 9.0m deep (28.0psia)
@10:54:48.55 Cartridge 42 filtering 476/1000ml at 0.30ml/s with 20.0psi vacuum 10.3m deep (29.9psia)
@10:55:48.55 Cartridge 42 filtering 494/1000ml at 0.29ml/s with 24.8psi vacuum 10.3m deep (29.9psia)
@10:56:48.55 Cartridge 42 filtering 512/1000ml at 0.30ml/s with 27.5psi vacuum 7.4m deep (25.6psia)
@10:57:48.56 Cartridge 42 filtering 529/1000ml at 0.31ml/s with 25.5psi vacuum 12.1m deep (32.6psia)
@10:58:48.55 Cartridge 42 filtering 546/1000ml at 0.28ml/s with 24.6psi vacuum 10.0m deep (29.4psia)
@10:59:48.56 Cartridge 42 filtering 564/1000ml at 0.27ml/s with 26.5psi vacuum 8.9m deep (27.9psia)
@11:00:48.55 Cartridge 42 filtering 581/1000ml at 0.29ml/s with 20.2psi vacuum 12.5m deep (33.2psia)
@11:01:48.56 Cartridge 42 filtering 597/1000ml at 0.28ml/s with 23.2psi vacuum 11.0m deep (30.9psia)
@11:02:48.55 Cartridge 42 filtering 614/1000ml at 0.27ml/s with 24.7psi vacuum 9.7m deep (29.0psia)
@11:03:48.55 Cartridge 42 filtering 631/1000ml at 0.27ml/s with 23.2psi vacuum 9.0m deep (28.0psia)
@11:04:48.55 Cartridge 42 filtering 647/1000ml at 0.26ml/s with 22.5psi vacuum 10.1m deep (29.7psia)
@11:05:48.56 Cartridge 42 filtering 663/1000ml at 0.26ml/s with 27.0psi vacuum 6.3m deep (24.1psia)
@11:06:48.55 Cartridge 42 filtering 680/1000ml at 0.27ml/s with 22.5psi vacuum 12.8m deep (33.6psia)
@11:07:48.56 Cartridge 42 filtering 696/1000ml at 0.28ml/s with 23.6psi vacuum 11.6m deep (31.8psia)
@11:08:48.55 Cartridge 42 filtering 712/1000ml at 0.26ml/s with 23.2psi vacuum 12.0m deep (32.5psia)
@11:09:48.56 Cartridge 42 filtering 728/1000ml at 0.26ml/s with 23.7psi vacuum 9.4m deep (28.6psia)
@11:10:48.55 Cartridge 42 filtering 743/1000ml at 0.26ml/s with 23.6psi vacuum 11.6m deep (31.9psia)
@11:11:48.56 Cartridge 42 filtering 759/1000ml at 0.26ml/s with 22.9psi vacuum 8.3m deep (27.0psia)
@11:12:48.55 Cartridge 42 filtering 774/1000ml at 0.24ml/s with 21.7psi vacuum 11.3m deep (31.4psia)
@11:13:48.56 Cartridge 42 filtering 790/1000ml at 0.25ml/s with 22.4psi vacuum 9.1m deep (28.2psia)
@11:14:48.55 Cartridge 42 filtering 805/1000ml at 0.25ml/s with 22.5psi vacuum 8.3m deep (27.0psia)
@11:15:48.56 Cartridge 42 filtering 820/1000ml at 0.23ml/s with 25.0psi vacuum 7.8m deep (26.2psia)
@11:16:48.56 Cartridge 42 filtering 835/1000ml at 0.25ml/s with 22.4psi vacuum 8.3m deep (26.9psia)
@11:17:48.56 Cartridge 42 filtering 850/1000ml at 0.24ml/s with 22.0psi vacuum 11.5m deep (31.7psia)
@11:18:48.55 Cartridge 42 filtering 864/1000ml at 0.23ml/s with 24.6psi vacuum 7.1m deep (25.2psia)
@11:19:48.56 Cartridge 42 filtering 879/1000ml at 0.25ml/s with 24.7psi vacuum 12.0m deep (32.4psia)
@11:20:48.55 Cartridge 42 filtering 894/1000ml at 0.23ml/s with 24.0psi vacuum 7.1m deep (25.2psia)
@11:21:48.56 Cartridge 42 filtering 908/1000ml at 0.23ml/s with 22.6psi vacuum 10.0m deep (29.5psia)
@11:22:48.55 Cartridge 42 filtering 922/1000ml at 0.24ml/s with 23.6psi vacuum 11.3m deep (31.4psia)
@11:23:48.94 Cartridge 42 filtering 936/1000ml at 0.21ml/s with 22.0psi vacuum 10.8m deep (30.7psia)
@11:24:48.55 Cartridge 42 filtering 951/1000ml at 0.25ml/s with 25.5psi vacuum 11.9m deep (32.3psia)
@11:25:48.56 Cartridge 42 filtering 965/1000ml at 0.24ml/s with 26.5psi vacuum 9.3m deep (28.5psia)
@11:26:48.55 Cartridge 42 filtering 978/1000ml at 0.22ml/s with 23.3psi vacuum 8.9m deep (27.9psia)
@11:27:48.56 Cartridge 42 filtering 992/1000ml at 0.22ml/s with 24.2psi vacuum 9.0m deep (28.0psia)
@11:28:22.77 <FILTERING> Pressure.stop
@11:28:22.89 <closeIntake> Intake.close
@11:28:22.94 <closeExhaust> Exhaust.close
@11:28:23.13 <FILTERING> Toroid.in :hold
@11:28:23.21 Toroid.seek :bypass
@11:28:24.84 Toroid.in :default
@11:28:24.91 Toroid.seek :clear
@11:28:26.53 Sampled  1000.0ml
@11:28:28.13 <LRAUV> -> Cmd.startProcessing
@11:28:28.56 <PROCESSING> Plunge.seek :reagent
@11:28:29.36 Sample loop closed. Ready to Deliver Reagents
@11:28:29.38 Archive.seek :sealed
@11:28:33.33 Archive.seek :evac
@11:28:47.37 delay 1:00
@11:29:47.40 Archive.seek :clear
@11:30:04.90 RNAlater soak for 10:00
@11:40:04.93 Archive.seek :tVent
@11:40:07.37 delay 1 second
@11:40:08.39 Archive.seek :evac
@11:40:23.91 delay 10 seconds
@11:40:33.94 Sample preserved and evacuated
@11:40:34.94 <LRAUV> -> Cmd.stop
@11:40:35.08 Carousel.in :default
@11:40:35.20 Carousel.home.seek 42
@11:40:35.50 Plunge.seek :locked
@11:40:36.41 <Archive> Archive.in :unlocking
@11:40:36.54 Archive.seek :clear
@11:40:36.61 <Air> Air.in :unlocking
@11:40:36.77 Air.seek :clear
@11:40:36.81 <Lysis1> Lysis1.in :unlocking
@11:40:36.98 Lysis1.seek :clear
@11:40:41.09 <LRAUV> Gate.power :motor,:OFF
@11:40:41.11 Gate.power :core,:OFF
@11:40:41.14 Gate.power :analytic1,:OFF
@11:40:41.17 Gate.power :analytic2,:OFF
@11:40:41.19 Gate.power :analytic3,:OFF
@11:40:41.21 Gate.power :main,:OFF
@11:40:41.23 Safely stopped and ready to power off
##-- file closed 2023-04-16T01:06:40.777Z