##-- file opened 2022-06-06T03:33:12.841Z @20:33:13.06 -> showStatus @20:33:15.08 -> Cmd.loadCartridge -6 @20:33:15.15 Gate.power :main,:ON,limit to 5000mA @20:33:15.16 Gate.power :analytic1,:ON,limit to 5000mA @20:33:15.18 Gate.power :analytic2,:ON,limit to 5000mA @20:33:15.21 Gate.power :analytic3,:ON,limit to 5000mA @20:33:15.35 Powering core bus @20:33:17.62 SPR Module 1, FW Rev. B, FPGA Rev. C @20:33:18.07 Archive.configure :default @20:33:18.20 Air.configure :default @20:33:18.43 Lysis1.configure :default @20:33:18.66 Lysis2.configure :default @20:33:18.94 Plunge.configure :default @20:33:19.22 SamplePump.configure :default @20:33:19.54 Toroid.configure :default @20:33:19.80 Carousel.configure :default @20:33:19.93 Pressure.configure :default @20:33:20.05 ExtraPump.configure :default @20:33:20.16 PinPort.configure :default @20:33:20.22 All elf controllers running firmware version 4.79 @20:33:20.24 Gate.power :motor,:ON,limit to 5000mA @20:33:20.46 Archive.in :default @20:33:20.53 Air.in :default @20:33:20.80 Archive.home.jog 0 @20:33:20.87 Air.home.jog 0 @20:33:21.07 PinPort.in :default @20:33:21.12 Toroid.in :default @20:33:21.16 Lysis2.in :default @20:33:21.22 Lysis1.in :default @20:33:21.29 Plunge.in :default @20:33:21.47 PinPort.home.jog 0 @20:33:21.52 Toroid.home.jog 0 @20:33:21.57 Plunge.home.jog 0 @20:33:21.59 Lysis2.home.jog 0 @20:33:21.62 Lysis1.home.jog 0 @20:33:21.95 Air.in :homing @20:33:21.99 Archive.in :homing @20:33:22.13 Air.jog 1391 @20:33:22.17 Archive.jog 1385 @20:33:22.34 Toroid.in :homing @20:33:22.37 Plunge.in :homing @20:33:22.42 PinPort.in :homing @20:33:22.58 Plunge.jog 1552 @20:33:22.64 PinPort.jog -107 @20:33:22.67 Toroid.jog 634 @20:33:22.86 Lysis2.in :homing @20:33:22.88 Lysis1.in :homing @20:33:22.99 Lysis2.jog 1391 @20:33:23.02 Lysis1.jog 1377 @20:33:23.13 PinPort.setRawPosition! 0 @20:33:23.18 PinPort.seek :home @20:33:23.27 PinPort.in :default @20:33:23.37 Air.setRawPosition! 50 @20:33:23.39 Archive.setRawPosition! 50 @20:33:23.46 Air.seek :home @20:33:23.51 Archive.seek :home @20:33:23.79 Air.in :default @20:33:23.88 Archive.in :default @20:33:23.97 Plunge.setRawPosition! 50 @20:33:24.02 Plunge.seek :home @20:33:24.19 Lysis2.setRawPosition! 50 @20:33:24.24 Lysis2.seek :home @20:33:24.35 Lysis1.setRawPosition! 50 @20:33:24.37 Plunge.in :default @20:33:24.46 Lysis1.seek :home @20:33:24.57 Lysis2.in :default @20:33:24.76 Lysis1.in :default @20:33:24.89 Toroid.setRawPosition! 0 @20:33:24.94 Toroid.seek :home @20:33:25.04 Toroid.in :default @20:33:25.17 Toroid.seek :clear @20:33:26.79 Carousel.in :default @20:33:27.28 Carousel.home.jog 0 @20:33:27.77 Carousel.in :default @20:33:27.84 Carousel.seek 25 @20:33:27.99 Selecting Cartridge 24 @20:33:28.25 Carousel.seek 24 @20:33:28.74 Lysis1.in :fast @20:33:28.80 Lysis1.seek :locked @20:33:31.41 Plunge.seek :locked @20:33:31.55 Air.in :fast @20:33:31.61 Air.seek :locked @20:33:34.08 Powering cartridge bus @20:33:36.67 Gate.setIR 1750,5000,0,6 @20:33:36.79 Gate.setIR 0,5000,0,6 @20:33:36.84 Gate.power :cartridge,:OFF @20:33:37.38 Mounted archive Cartridge @20:33:54.68 -> Cmd.startFiltering @20:33:54.92 Preparing to filter cartridge 24 @20:33:55.19 Priming sample loop w/100ml, bypass w/10ml @20:33:55.21 SamplePump.setPosition! 0ml @20:33:55.26 Bypass.open @20:33:56.69 Intake.open @20:33:57.40 Sea 9.6m deep (28.9psia) @20:33:57.43 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @20:34:00.00 Vacuum tare is 54% of full scale @20:34:00.14 Pressure.in :default @20:34:00.20 Pressure.hold @20:34:00.38 SamplePump.in :prime @20:34:00.45 SamplePump.seek 10ml,59 seconds @20:34:09.06 Pressure.stop @20:34:09.11 SamplePump.setPosition! 0ml @20:34:09.17 Exhaust.open @20:34:10.65 Bypass.close @20:34:12.12 Pressure.in :default @20:34:12.18 Pressure.hold @20:34:12.29 Sea 9.6m deep (28.9psia) @20:34:12.43 SamplePump.in :prime @20:34:12.50 SamplePump.seek 100ml,5:16 @20:35:46.34 Pressure.stop @20:35:46.41 Bypass.open @20:35:47.08 Intake.close @20:35:47.84 Toroid.in :hold @20:35:47.90 Toroid.seek :crack @20:35:49.41 Toroid.seek :cartridge @20:35:50.49 Toroid.in :default @20:35:50.55 Toroid.seek :clear @20:35:52.51 Pressurizing cartridge to 26psi above 28.8psia @20:35:52.53 SamplePump.setPosition! 0ml @20:35:52.58 Intake.open @20:35:53.28 Bypass.close @20:35:54.02 Exhaust.close @20:35:54.86 Pressure.in :default @20:35:54.93 Pressure.hold @20:35:55.11 SamplePump.in :charge @20:35:55.17 SamplePump.seek 2.3ml,42 seconds @20:36:01.07 Pressure.stop @20:36:01.13 Intake.close @20:36:01.89 Pressurized cartridge with 0.165ml to 26.1psi @20:36:01.90 Isolated @49.8psia (21.0psi) @20:36:01.91 Verifying cartridge holds at least 0.65..0.85 of 49.8psia @20:36:01.95 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @20:36:06.58 Sampling 1000.0ml @20:36:06.59 SamplePump.setPosition! 0ml @20:36:06.64 Exhaust.open @20:36:07.37 Intake.open @20:36:08.16 Pressure.in :default @20:36:08.22 Pressure.hold @20:36:08.33 Sea 10.0m deep (29.5psia) @20:36:08.47 SamplePump.in :sample @20:36:08.54 SamplePump.seek 1000ml,1:23:48 @20:36:11.19 Cartridge 24 filtering 1/1000ml at 0.42ml/s with 22.2psi vacuum 9.4m deep (28.6psia) @20:37:11.19 Cartridge 24 filtering 25/1000ml at 0.40ml/s with 26.0psi vacuum 7.9m deep (26.4psia) @20:38:11.20 Cartridge 24 filtering 46/1000ml at 0.34ml/s with 22.6psi vacuum 7.6m deep (26.0psia) @20:39:11.19 Cartridge 24 filtering 67/1000ml at 0.33ml/s with 22.7psi vacuum 7.1m deep (25.2psia) @20:40:11.20 Cartridge 24 filtering 87/1000ml at 0.30ml/s with 23.3psi vacuum 7.5m deep (25.8psia) @20:41:11.19 Cartridge 24 filtering 105/1000ml at 0.31ml/s with 25.8psi vacuum 7.5m deep (25.8psia) @20:42:11.20 Cartridge 24 filtering 123/1000ml at 0.28ml/s with 23.4psi vacuum 7.2m deep (25.4psia) @20:43:11.19 Cartridge 24 filtering 139/1000ml at 0.29ml/s with 25.4psi vacuum 7.0m deep (25.0psia) @20:44:11.20 Cartridge 24 filtering 155/1000ml at 0.23ml/s with 23.3psi vacuum 7.4m deep (25.7psia) @20:45:11.20 Cartridge 24 filtering 170/1000ml at 0.22ml/s with 25.5psi vacuum 7.7m deep (26.1psia) @20:46:11.20 Cartridge 24 filtering 184/1000ml at 0.22ml/s with 23.7psi vacuum 6.0m deep (23.5psia) @20:47:11.19 Cartridge 24 filtering 197/1000ml at 0.20ml/s with 26.2psi vacuum 7.1m deep (25.2psia) @20:48:11.20 Cartridge 24 filtering 209/1000ml at 0.20ml/s with 25.5psi vacuum 7.3m deep (25.5psia) @20:49:11.19 Cartridge 24 filtering 221/1000ml at 0.20ml/s with 25.0psi vacuum 6.4m deep (24.1psia) @20:49:20.85 Flow averaged 0.28ml/s over 13:13 @20:49:20.89 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @20:49:21.47 Relieved excess vacuum in .6 seconds @20:49:21.49 SamplePump.seek 1000ml,1:05:12 @20:49:40.92 Flow averaged 0.20ml/s over 20 seconds @20:49:40.95 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @20:49:41.53 Relieved excess vacuum in .6 seconds @20:49:41.55 SamplePump.seek 1000ml,1:04:53 @20:49:54.78 Flow averaged 0.20ml/s over 14 seconds @20:49:54.80 Intake.close @20:49:54.84 Exhaust.close @20:49:55.06 Toroid.in :hold @20:49:55.13 Toroid.seek :bypass @20:49:56.75 Toroid.in :default @20:49:56.81 Toroid.seek :clear @20:49:58.41 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @20:49:58.43 Pressure.stop @20:49:58.62 Sampled 229.8ml @20:50:00.67 -> Cmd.startProcessing @20:50:01.52 Plunge.seek :reagent @20:50:02.33 Sample loop closed. Ready to Deliver Reagents @20:50:02.34 Archive.seek :sealed @20:50:06.32 Archive.seek :evac @20:50:20.35 delay 1:00 @20:51:20.37 Archive.seek :clear @20:51:37.85 RNAlater soak for 10:00 @21:01:37.87 Archive.seek :tVent @21:01:40.32 delay 1 second @21:01:41.34 Archive.seek :evac @21:01:56.85 delay 10 seconds @21:02:06.87 Sample preserved and evacuated @21:02:07.92 -> Cmd.stop @21:02:08.07 Carousel.seek 24 @21:02:08.30 Plunge.seek :locked @21:02:09.23 Archive.in :unlocking @21:02:09.37 Air.in :unlocking @21:02:09.41 Archive.seek :clear @21:02:09.56 Air.seek :clear @21:02:09.65 Lysis1.in :unlocking @21:02:09.77 Lysis1.seek :clear @21:02:13.57 Gate.power :motor,:OFF @21:02:13.59 Gate.power :core,:OFF @21:02:13.61 Gate.power :analytic1,:OFF @21:02:13.64 Gate.power :analytic2,:OFF @21:02:13.66 Gate.power :analytic3,:OFF @21:02:13.68 Gate.power :main,:OFF @21:02:13.70 Safely stopped and ready to power off ##-- file closed 2022-06-06T04:14:22.530Z ##-- file opened 2022-06-06T04:14:22.532Z @21:14:23.01 -> showStatus @21:14:25.02 -> Cmd.loadCartridge -10 @21:14:25.09 Gate.power :main,:ON,limit to 5000mA @21:14:25.10 Gate.power :analytic1,:ON,limit to 5000mA @21:14:25.13 Gate.power :analytic2,:ON,limit to 5000mA @21:14:25.15 Gate.power :analytic3,:ON,limit to 5000mA @21:14:25.30 Powering core bus @21:14:27.56 SPR Module 1, FW Rev. B, FPGA Rev. C @21:14:28.18 Archive.configure :default @21:14:28.47 Air.configure :default @21:14:28.73 Lysis1.configure :default @21:14:28.98 Lysis2.configure :default @21:14:29.28 Plunge.configure :default @21:14:29.49 SamplePump.configure :default @21:14:29.62 Toroid.configure :default @21:14:29.75 Carousel.configure :default @21:14:29.88 Pressure.configure :default @21:14:30.00 ExtraPump.configure :default @21:14:30.11 PinPort.configure :default @21:14:30.17 All elf controllers running firmware version 4.79 @21:14:30.20 Gate.power :motor,:ON,limit to 5000mA @21:14:30.34 Archive.in :default @21:14:30.67 Archive.home.jog 0 @21:14:30.87 Toroid.in :default @21:14:30.91 Plunge.in :default @21:14:30.95 Lysis1.in :default @21:14:31.01 Air.in :default @21:14:31.13 PinPort.in :default @21:14:31.16 Lysis2.in :default @21:14:31.35 Toroid.home.jog 0 @21:14:31.39 Plunge.home.jog 0 @21:14:31.41 Lysis1.home.jog 0 @21:14:31.44 PinPort.home.jog 0 @21:14:31.47 Lysis2.home.jog 0 @21:14:31.49 Air.home.jog 0 @21:14:31.91 Archive.in :homing @21:14:32.07 Plunge.in :homing @21:14:32.18 Archive.jog 1389 @21:14:32.20 Toroid.in :homing @21:14:32.24 PinPort.in :homing @21:14:32.43 Toroid.jog 618 @21:14:32.46 PinPort.jog -107 @21:14:32.49 Plunge.jog 1551 @21:14:32.69 Lysis2.in :homing @21:14:32.71 Lysis1.in :homing @21:14:32.74 Air.in :homing @21:14:32.88 Lysis2.jog 1391 @21:14:32.90 Lysis1.jog 1380 @21:14:32.93 Air.jog 1387 @21:14:33.09 PinPort.setRawPosition! 0 @21:14:33.13 PinPort.seek :home @21:14:33.22 PinPort.in :default @21:14:33.48 Archive.setRawPosition! 50 @21:14:33.53 Archive.seek :home @21:14:33.79 Plunge.setRawPosition! 50 @21:14:33.84 Plunge.seek :home @21:14:33.93 Archive.in :default @21:14:34.13 Lysis2.setRawPosition! 50 @21:14:34.18 Lysis2.seek :home @21:14:34.28 Lysis1.setRawPosition! 50 @21:14:34.31 Plunge.in :default @21:14:34.33 Air.setRawPosition! 50 @21:14:34.43 Lysis1.seek :home @21:14:34.52 Air.seek :home @21:14:34.66 Toroid.setRawPosition! 0 @21:14:34.68 Lysis2.in :default @21:14:34.77 Toroid.seek :home @21:14:34.90 Lysis1.in :default @21:14:34.92 Air.in :default @21:14:34.95 Toroid.in :default @21:14:35.42 Toroid.seek :clear @21:14:37.42 Carousel.in :default @21:14:37.48 Carousel.home.jog 0 @21:14:37.97 Carousel.in :default @21:14:38.04 Carousel.seek 24 @21:14:38.19 Selecting Cartridge 23 @21:14:38.45 Carousel.seek 23 @21:14:38.93 Lysis1.in :fast @21:14:38.99 Lysis1.seek :locked @21:14:41.59 Plunge.seek :locked @21:14:41.72 Air.in :fast @21:14:41.78 Air.seek :locked @21:14:44.24 Powering cartridge bus @21:14:46.84 Gate.setIR 1750,5000,0,6 @21:14:46.96 Gate.setIR 0,5000,0,6 @21:14:47.03 PuckHeater.configure :default @21:14:47.08 Gate.power :heat,:ON,limit to 5000mA @21:14:47.76 Mounted lysis Cartridge board#Q05087 @21:14:47.80 Using default puck heat loss curve for HeatingAccelInCarousel @21:15:05.42 -> Cmd.startFiltering @21:15:05.67 Preparing to filter cartridge 23 @21:15:05.93 Priming sample loop w/100ml, bypass w/10ml @21:15:05.94 SamplePump.setPosition! 0ml @21:15:05.99 Bypass.open @21:15:07.45 Intake.open @21:15:08.21 Sea 10.0m deep (29.4psia) @21:15:08.23 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:15:10.80 Vacuum tare is 54% of full scale @21:15:10.93 Pressure.in :default @21:15:10.99 Pressure.hold @21:15:11.17 SamplePump.in :prime @21:15:11.24 SamplePump.seek 10ml,59 seconds @21:15:19.85 Pressure.stop @21:15:19.90 SamplePump.setPosition! 0ml @21:15:19.96 Exhaust.open @21:15:21.39 Bypass.close @21:15:22.92 Pressure.in :default @21:15:22.99 Pressure.hold @21:15:23.10 Sea 10.0m deep (29.5psia) @21:15:23.24 SamplePump.in :prime @21:15:23.31 SamplePump.seek 100ml,5:16 @21:16:56.68 Pressure.stop @21:16:56.74 Bypass.open @21:16:57.41 Intake.close @21:16:58.18 Toroid.in :hold @21:16:58.24 Toroid.seek :crack @21:16:59.75 Toroid.seek :cartridge @21:17:00.84 Toroid.in :default @21:17:00.90 Toroid.seek :clear @21:17:02.86 Pressurizing cartridge to 26psi above 29.9psia @21:17:02.88 SamplePump.setPosition! 0ml @21:17:02.92 Intake.open @21:17:03.58 Bypass.close @21:17:04.25 Exhaust.close @21:17:05.06 Pressure.in :default @21:17:05.12 Pressure.hold @21:17:05.29 SamplePump.in :charge @21:17:05.36 SamplePump.seek 2.3ml,42 seconds @21:17:13.77 Pressure.stop @21:17:13.82 Intake.close @21:17:14.52 Pressurized cartridge with 0.338ml to 26.3psi @21:17:14.53 Isolated @54.5psia (24.6psi) @21:17:14.55 Verifying cartridge holds at least 0.65..0.85 of 54.5psia @21:17:14.59 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @21:17:19.22 Sampling 1000.0ml @21:17:19.24 SamplePump.setPosition! 0ml @21:17:19.29 Exhaust.open @21:17:20.03 Intake.open @21:17:20.83 Pressure.in :default @21:17:20.89 Pressure.hold @21:17:21.00 Sea 9.9m deep (29.4psia) @21:17:21.14 SamplePump.in :sample @21:17:21.21 SamplePump.seek 1000ml,1:23:48 @21:17:23.86 Cartridge 23 filtering 1/1000ml at 0.41ml/s with 23.8psi vacuum 9.0m deep (28.0psia) @21:18:23.86 Cartridge 23 filtering 26/1000ml at 0.40ml/s with 25.0psi vacuum 8.6m deep (27.4psia) @21:19:23.87 Cartridge 23 filtering 50/1000ml at 0.38ml/s with 24.4psi vacuum 8.8m deep (27.6psia) @21:20:23.86 Cartridge 23 filtering 73/1000ml at 0.35ml/s with 25.2psi vacuum 8.1m deep (26.7psia) @21:21:23.87 Cartridge 23 filtering 94/1000ml at 0.33ml/s with 25.0psi vacuum 9.7m deep (29.0psia) @21:22:23.86 Cartridge 23 filtering 115/1000ml at 0.32ml/s with 22.4psi vacuum 7.9m deep (26.4psia) @21:23:23.86 Cartridge 23 filtering 135/1000ml at 0.30ml/s with 24.7psi vacuum 9.6m deep (28.8psia) @21:24:23.86 Cartridge 23 filtering 154/1000ml at 0.34ml/s with 25.4psi vacuum 8.3m deep (27.0psia) @21:25:23.86 Cartridge 23 filtering 173/1000ml at 0.30ml/s with 20.9psi vacuum 9.2m deep (28.3psia) @21:26:23.86 Cartridge 23 filtering 191/1000ml at 0.28ml/s with 23.6psi vacuum 8.3m deep (26.9psia) @21:27:23.87 Cartridge 23 filtering 208/1000ml at 0.26ml/s with 23.5psi vacuum 7.1m deep (25.2psia) @21:28:23.86 Cartridge 23 filtering 225/1000ml at 0.26ml/s with 22.3psi vacuum 9.3m deep (28.5psia) @21:29:23.87 Cartridge 23 filtering 241/1000ml at 0.28ml/s with 22.3psi vacuum 9.0m deep (28.1psia) @21:30:23.87 Cartridge 23 filtering 257/1000ml at 0.23ml/s with 23.3psi vacuum 8.6m deep (27.4psia) @21:31:23.86 Cartridge 23 filtering 272/1000ml at 0.25ml/s with 25.8psi vacuum 8.4m deep (27.1psia) @21:32:23.87 Cartridge 23 filtering 286/1000ml at 0.23ml/s with 25.5psi vacuum 8.0m deep (26.5psia) @21:33:23.86 Cartridge 23 filtering 300/1000ml at 0.21ml/s with 24.1psi vacuum 8.4m deep (27.2psia) @21:34:23.87 Cartridge 23 filtering 314/1000ml at 0.21ml/s with 24.4psi vacuum 8.8m deep (27.7psia) @21:35:23.87 Cartridge 23 filtering 327/1000ml at 0.21ml/s with 23.5psi vacuum 8.4m deep (27.1psia) @21:36:23.87 Cartridge 23 filtering 339/1000ml at 0.23ml/s with 23.3psi vacuum 8.4m deep (27.1psia) @21:37:23.86 Cartridge 23 filtering 351/1000ml at 0.20ml/s with 26.2psi vacuum 9.4m deep (28.6psia) @21:38:23.87 Cartridge 23 filtering 364/1000ml at 0.20ml/s with 25.9psi vacuum 7.7m deep (26.0psia) @21:38:51.77 Flow averaged 0.29ml/s over 21:31 @21:38:51.81 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi @21:38:52.63 Relieved excess vacuum in .9 seconds @21:38:52.66 SamplePump.seek 1000ml,53:03 @21:39:23.86 Cartridge 23 filtering 375/1000ml at 0.20ml/s with 26.9psi vacuum 9.0m deep (28.0psia) @21:39:27.02 Flow averaged 0.20ml/s over 35 seconds @21:39:27.04 Intake.close @21:39:27.11 Exhaust.close @21:39:27.30 Toroid.in :hold @21:39:27.36 Toroid.seek :bypass @21:39:29.41 Toroid.in :default @21:39:29.47 Toroid.seek :clear @21:39:31.08 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @21:39:31.09 Pressure.stop @21:39:31.29 Sampled 376.1ml @21:39:33.25 -> Cmd.startProcessing @21:39:34.69 SPR.command \"read_sequences\" @21:39:35.08 SPR.command \"stats_on\" @21:39:35.19 SPR.command \"start 0 1\" @21:39:35.93 SPR.start /var/log/esp/spr22jun0521h.spr.bz2 @21:39:38.41 Handoff.select :waste @21:39:45.10 Syringe tare is 19% of full scale @21:39:45.12 PinPort.in :locking @21:39:45.20 PinPort.seek :locked @21:39:45.68 Archive.in :fast @21:39:45.73 Lysis2.in :fast @21:39:45.80 Lysis1.seek 1350uL @21:39:45.92 Archive.seek :full @21:39:46.00 Lysis2.seek :full @21:39:48.02 Plunge.seek :reagent @21:39:48.90 Evacuating puck with filter bubble point of 50psi @21:39:48.92 PinPort.seek :locked @21:39:49.01 Handoff.select :waste @21:39:56.59 Air syringe pumping up to 1 stroke to reach 8psi @21:39:56.60 Air stroke #1 of 1 @21:39:56.70 Air.in :pressurize @21:39:56.76 Air.seek :pressurized @21:40:08.33 0.4psi after pressurize @21:40:08.35 Air.in :compress @21:40:08.43 Air.seek :evac @21:40:12.53 1.0psi after compress @21:40:25.11 0.0psi after compress (stable) @21:40:25.12 Air.in :default @21:40:25.19 Air.hold @21:40:25.36 at -0.0psi Opened to waste @21:40:25.37 Handoff.select :blocked @21:40:25.51 Archive syringe pumping up to 8 strokes to reach 50psi @21:40:25.52 Archive stroke #1 of 8 @21:40:25.61 Archive.in :pressurize @21:40:25.69 Archive.seek :pressurized @21:40:38.56 0.9psi after pressurize @21:40:38.58 Archive.in :compress @21:40:38.65 Archive.seek :empty @21:40:53.33 19.7psi after compress @21:41:07.90 25.0psi after compress (stable) @21:41:07.92 Archive.in :gulp @21:41:08.00 Archive.seek :topVent @21:41:15.16 25.1psi after gulp @21:41:27.73 24.9psi after gulp (stable) @21:41:27.74 Archive stroke #2 of 8 @21:41:27.84 Archive.in :pressurize @21:41:27.90 Archive.seek :pressurized @21:41:40.75 24.6psi after pressurize @21:41:40.77 Archive.in :compress @21:41:40.84 Archive.seek :empty @21:41:55.54 26.1psi after compress @21:42:08.13 26.2psi after compress (stable) @21:42:08.15 Archive.in :gulp @21:42:08.23 Archive.seek :topVent @21:42:15.39 26.7psi after gulp @21:42:27.97 26.7psi after gulp (stable) @21:42:27.98 Archive stroke #3 of 8 @21:42:28.08 Archive.in :pressurize @21:42:28.14 Archive.seek :pressurized @21:42:41.03 26.5psi after pressurize @21:42:41.05 Archive.in :compress @21:42:41.12 Archive.seek :empty @21:42:55.80 28.0psi after compress @21:43:08.41 29.2psi after compress (stable) @21:43:08.44 Archive.in :gulp @21:43:08.52 Archive.seek :topVent @21:43:15.67 30.6psi after gulp @21:43:28.27 31.2psi after gulp (stable) @21:43:28.29 Archive stroke #4 of 8 @21:43:28.38 Archive.in :pressurize @21:43:28.46 Archive.seek :pressurized @21:43:41.31 31.8psi after pressurize @21:43:41.33 Archive.in :compress @21:43:41.40 Archive.seek :empty @21:43:56.09 36.1psi after compress @21:44:06.81 Reached 50.1 of 50.0psi after pumping 6920uL (4.00 strokes) @21:44:06.82 Archive.in :default @21:44:06.90 Archive.hold @21:44:06.97 Handoff.select :waste @21:44:24.68 delay 1:00 @21:45:24.69 Handoff.select :blocked @21:45:47.36 Handoff.select :waste @21:45:47.48 1.5psi at evacEnd @21:45:47.49 Handoff.select :blocked @21:45:47.63 Archive.in :fast @21:45:47.69 Archive.seek :topVent @21:46:01.35 Stable @0.9psi after Handoff blocked @21:46:01.37 Archive syringe pumping up to 8 strokes to reach 31psi @21:46:01.38 Archive stroke #1 of 8 @21:46:01.50 Archive.in :pressurize @21:46:01.57 Archive.seek :pressurized @21:46:14.42 1.7psi after pressurize @21:46:14.44 Archive.in :compress @21:46:14.51 Archive.seek :empty @21:46:29.19 4.8psi after compress @21:46:56.76 12.8psi after compress (stable) @21:46:56.78 Archive.in :gulp @21:46:56.86 Archive.seek :topVent @21:47:04.02 13.8psi after gulp @21:47:16.59 14.7psi after gulp (stable) @21:47:16.60 Archive stroke #2 of 8 @21:47:16.70 Archive.in :pressurize @21:47:16.76 Archive.seek :pressurized @21:47:29.61 15.8psi after pressurize @21:47:29.63 Archive.in :compress @21:47:29.71 Archive.seek :empty @21:47:44.40 19.2psi after compress @21:48:11.98 27.6psi after compress (stable) @21:48:12.01 Archive.in :gulp @21:48:12.10 Archive.seek :topVent @21:48:19.26 28.6psi after gulp @21:48:31.86 29.5psi after gulp (stable) @21:48:31.88 Archive stroke #3 of 8 @21:48:31.98 Archive.in :pressurize @21:48:32.05 Archive.seek :pressurized @21:48:44.91 30.4psi after pressurize @21:48:44.93 Archive.in :compress @21:48:45.00 Archive.seek :empty @21:48:55.42 Reached 30.9 of 30.9psi after pumping 5098uL (2.95 strokes) @21:48:55.43 Archive.in :default @21:48:55.54 Archive.hold @21:48:55.61 Handoff.select :waste @21:49:02.79 delay 1:00 @21:50:02.80 Handoff.select :blocked @21:50:25.48 Handoff.select :waste @21:50:25.59 1.9psi at evacEnd @21:50:25.61 Air.seek :fullVent @21:50:27.04 Archive.in :fast @21:50:27.10 Archive.seek :topVent @21:50:33.88 Seawater evacuated, Presure above/below filter released. Lysis begins @21:50:33.97 Lysis1.in :slow @21:50:34.03 Lysis1.seek 1050uL @21:51:04.05 delay 5 seconds @21:51:09.07 Handoff.select :blocked @21:51:09.25 Lysis1.seek 450uL @21:52:09.22 delay 3 seconds @21:52:12.59 Air.in mix with 1.40048 parts Lysis1 @21:52:13.01 Air.seek :evac @21:52:13.04 Lysis1.seek 300uL @21:52:28.16 Lysis1.seek :empty @21:52:58.26 Lysis buffer delivered @25.3psi @21:52:58.28 Polling Can every 2:00 @21:52:58.82 PuckHeater.configure :default #ambient 16.4C @21:52:58.87 PuckHeater.seek 80C @21:54:03.99 PuckHeater.hold 2:00,80C @21:56:04.12 Polling Can every 2:00 @21:56:04.66 PuckHeater.configure :default #ambient 16.5C @21:56:04.71 PuckHeater.hold 30 seconds,79C @21:56:34.91 Polling Can every 2:00 @21:56:35.45 PuckHeater.configure :default #ambient 16.5C @21:56:35.50 PuckHeater.hold 30 seconds,78.5C @21:57:05.71 Polling Can every 2:00 @21:57:06.25 PuckHeater.configure :default #ambient 16.5C @21:57:06.31 PuckHeater.hold 10:00,77.9C @22:07:06.75 Lysis complete. Syringe pressure @31.0psi @22:07:06.76 Handoff.select :blocked @22:07:06.80 Archive syringe pumping up to 5 strokes to reach 40psi @22:07:06.82 Archive stroke #1 of 5 @22:07:06.91 Archive.in :pressurize @22:07:06.98 Archive.seek :pressurized @22:07:19.85 30.5psi after pressurize @22:07:19.87 Archive.in :compress @22:07:19.94 Archive.seek :empty @22:07:27.87 Reached 42.9 of 40.0psi after pumping 1584uL (0.92 strokes) @22:07:27.89 Archive.in :default @22:07:27.96 Archive.hold @22:07:28.03 delay 2 seconds @22:07:30.05 Archive.in :fast @22:07:30.11 Archive.seek :full @22:07:36.77 Lysis1.seek 138uL @22:07:50.64 delay 1 second @22:07:51.71 Archive.seek :full @22:07:51.76 Lysis1.seek :empty @22:08:05.74 Initial air pressure @53.6psi @22:08:05.81 Lysis1.in :extract @22:08:05.90 Lysis1.seek 600uL,nil @22:08:10.41 Archive syringe pumping up to 6 strokes to reach 25psi @22:08:10.42 Archive stroke #1 of 6 @22:08:10.52 Archive.in :pressurize @22:08:10.60 Archive.seek :pressurized @22:08:23.53 14.2psi after pressurize @22:08:23.55 Archive.in :compress @22:08:23.66 Archive.seek :empty @22:08:31.00 Syringe::LysatePlungerStuck in PROCESSING -- at < 332uL during Lysis Syringe #1 (LyS1) move @22:08:31.03 Lysis1.in :default @22:08:31.10 Lysis1.seek 297uL @22:08:31.73 Reached 26.3 of 25.0psi after pumping 1588uL (0.92 strokes) @22:08:31.74 Archive.in :default @22:08:31.81 Archive.hold @22:08:32.93 Lysis1.in :extract @22:08:33.00 Lysis1.seek 600uL,nil @22:08:38.58 Initial 600uL lysate @19.0psi @22:08:38.59 Handoff.select :blocked @22:08:38.71 Initial air pressure @20.2psi @22:08:38.79 Lysis1.seek 825uL,nil @22:08:40.54 Archive syringe pumping up to 6 strokes to reach 17psi @22:08:40.55 Archive stroke #1 of 6 @22:08:40.71 Archive.in :compress @22:08:40.78 Archive.seek :empty @22:08:45.14 Archive.in :default @22:08:45.19 Archive.hold @22:08:45.43 Recovered 825uL lysate @16.8psi @22:08:45.45 Air.in :halfSpeed @22:08:45.52 Air.seek :fullVent @22:08:47.72 Puck Top Vented, bottom @6.7psi @22:08:47.73 Handoff.select :waste @22:08:47.87 delay 5 seconds @22:08:52.96 Handoff open to waste, bottom @0.2psi @22:08:52.99 Air.in :default @22:08:53.06 Air.seek :evac @22:08:54.47 Lysis2.in :DAmix @22:08:54.58 Lysis1.in :DAmix @22:08:55.04 Lysis2.seek 1480uL @22:08:55.08 Lysis1.seek 575uL @22:09:30.15 delay 5 seconds @22:09:35.17 Handoff.select SPR @22:09:35.29 SPR.command \"start 2 14\" @22:09:35.95 SPR stabilized @22:09:36.09 delay 10 seconds @22:09:46.11 SPR.command \"start 1 0\" @22:09:49.80 Waiting up to 4:00 for SPR to enter state 1 @22:11:52.64 SPR.command \"hold_on\" @22:11:54.53 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @22:11:54.98 Lysis2.seek 1180uL @22:11:55.03 Lysis1.seek 275uL @22:12:37.05 delay 15 seconds @22:12:52.08 SPR.command \"hold_off\" @22:12:54.70 Waiting up to 38:00 for SPR to enter state 7 @22:33:43.58 SPRsummary:-6.99467,106.893,116.749,145.355 after 3406s @22:40:20.81 SPR analysis completed @22:40:27.58 Ended log spr22jun0521h.spr.bz2 @22:40:27.59 SPR.command \"stats_off\" @22:40:29.11 -> Cmd.stop @22:40:29.20 Handoff.select :close @22:40:29.34 Gate.power :heat,:OFF @22:40:29.35 Gate.power :cartridge,:OFF @22:40:29.44 Carousel.seek 23 @22:40:29.63 Plunge.seek :locked @22:40:30.49 PinPort.in :unlocking @22:40:30.56 PinPort.seek :clear @22:40:31.10 Archive.in :unlocking @22:40:31.22 Archive.seek :clear @22:40:31.33 Air.in :unlocking @22:40:31.42 Air.seek :clear @22:40:31.54 Lysis1.in :unlocking @22:40:31.62 Lysis1.seek :clear @22:40:31.66 Lysis2.in :unlocking @22:40:31.76 Lysis2.seek :clear @22:40:35.31 Gate.power :motor,:OFF @22:40:35.32 Gate.power :core,:OFF @22:40:35.35 Gate.power :analytic1,:OFF @22:40:35.37 Gate.power :analytic2,:OFF @22:40:35.39 Gate.power :analytic3,:OFF @22:40:35.42 Gate.power :main,:OFF @22:40:35.44 Safely stopped and ready to power off ##-- file closed 2022-06-06T13:04:37.545Z ##-- file opened 2022-06-06T13:04:37.549Z @06:04:38.34 -> showStatus @06:04:40.36 -> Cmd.loadCartridge -6 @06:04:40.42 Gate.power :main,:ON,limit to 5000mA @06:04:40.45 Gate.power :analytic1,:ON,limit to 5000mA @06:04:40.47 Gate.power :analytic2,:ON,limit to 5000mA @06:04:40.50 Gate.power :analytic3,:ON,limit to 5000mA @06:04:40.65 Powering core bus @06:04:42.97 SPR Module 1, FW Rev. B, FPGA Rev. C @06:04:43.59 Archive.configure :default @06:04:43.75 Air.configure :default @06:04:43.85 Lysis1.configure :default @06:04:43.96 Lysis2.configure :default @06:04:44.09 Plunge.configure :default @06:04:44.21 SamplePump.configure :default @06:04:44.34 Toroid.configure :default @06:04:44.46 Carousel.configure :default @06:04:44.59 Pressure.configure :default @06:04:44.72 ExtraPump.configure :default @06:04:44.82 PinPort.configure :default @06:04:44.88 All elf controllers running firmware version 4.79 @06:04:44.91 Gate.power :motor,:ON,limit to 5000mA @06:04:45.11 Archive.in :default @06:04:45.39 Archive.home.jog 0 @06:04:45.58 Toroid.in :default @06:04:45.66 Plunge.in :default @06:04:45.70 Lysis1.in :default @06:04:45.78 Air.in :default @06:04:45.81 PinPort.in :default @06:04:45.84 Lysis2.in :default @06:04:46.09 Toroid.home.jog 0 @06:04:46.12 Plunge.home.jog 0 @06:04:46.15 Lysis1.home.jog 0 @06:04:46.18 PinPort.home.jog 0 @06:04:46.20 Air.home.jog 0 @06:04:46.27 Lysis2.home.jog 0 @06:04:46.72 Archive.in :homing @06:04:46.93 Toroid.in :homing @06:04:46.96 Plunge.in :homing @06:04:46.99 Archive.jog 1389 @06:04:47.03 PinPort.in :homing @06:04:47.06 Lysis1.in :homing @06:04:47.10 Air.in :homing @06:04:47.14 Lysis2.in :homing @06:04:47.33 Toroid.jog 627 @06:04:47.37 Plunge.jog 1550 @06:04:47.40 PinPort.jog -107 @06:04:47.43 Lysis1.jog 1377 @06:04:47.47 Lysis2.jog 1392 @06:04:47.54 Air.jog 1389 @06:04:47.80 PinPort.setRawPosition! 0 @06:04:47.85 PinPort.seek :home @06:04:47.94 PinPort.in :default @06:04:48.35 Archive.setRawPosition! 50 @06:04:48.40 Archive.seek :home @06:04:48.68 Archive.in :default @06:04:48.81 Lysis2.setRawPosition! 50 @06:04:48.84 Air.setRawPosition! 50 @06:04:48.87 Lysis1.setRawPosition! 50 @06:04:48.91 Plunge.setRawPosition! 50 @06:04:49.01 Lysis2.seek :home @06:04:49.06 Air.seek :home @06:04:49.11 Lysis1.seek :home @06:04:49.15 Plunge.seek :home @06:04:49.40 Lysis2.in :default @06:04:49.51 Air.in :default @06:04:49.62 Plunge.in :default @06:04:49.66 Lysis1.in :default @06:04:49.69 Toroid.setRawPosition! 0 @06:04:49.83 Toroid.seek :home @06:04:49.92 Toroid.in :default @06:04:50.52 Toroid.seek :clear @06:04:52.14 Carousel.in :default @06:04:52.21 Carousel.home.jog 0 @06:04:52.68 Carousel.in :default @06:04:52.74 Carousel.seek 23 @06:04:52.91 Selecting Cartridge 22 @06:04:53.16 Carousel.seek 22 @06:04:53.65 Lysis1.in :fast @06:04:53.72 Lysis1.seek :locked @06:04:56.34 Plunge.seek :locked @06:04:56.47 Air.in :fast @06:04:56.54 Air.seek :locked @06:04:59.00 Powering cartridge bus @06:05:01.61 Gate.setIR 1750,5000,0,6 @06:05:01.73 Gate.setIR 0,5000,0,6 @06:05:01.78 Gate.power :cartridge,:OFF @06:05:02.31 Mounted archive Cartridge @06:05:19.95 -> Cmd.startFiltering @06:05:20.20 Preparing to filter cartridge 22 @06:05:20.46 Priming sample loop w/100ml, bypass w/10ml @06:05:20.48 SamplePump.setPosition! 0ml @06:05:20.53 Bypass.open @06:05:21.97 Intake.open @06:05:22.69 Sea 15.3m deep (37.3psia) @06:05:22.71 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:05:25.28 Vacuum tare is 54% of full scale @06:05:25.41 Intake.close @06:05:25.45 Bypass.close @06:05:26.21 Verifying toroid holds at least 0.6..0.85 of 37.4psia @06:05:26.22 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @06:05:30.80 Toroid pressure fell 2% to 36.6psia @06:05:30.81 Bypass.open @06:05:31.49 Intake.open @06:05:32.87 Pressure.in :default @06:05:32.93 Pressure.hold @06:05:33.15 SamplePump.in :prime @06:05:33.23 SamplePump.seek 10ml,59 seconds @06:05:41.84 Pressure.stop @06:05:41.89 SamplePump.setPosition! 0ml @06:05:41.94 Exhaust.open @06:05:43.42 Bypass.close @06:05:44.94 Pressure.in :default @06:05:45.00 Pressure.hold @06:05:45.11 Sea 14.0m deep (35.4psia) @06:05:45.25 SamplePump.in :prime @06:05:45.32 SamplePump.seek 100ml,5:16 @06:07:19.36 Pressure.stop @06:07:19.42 Bypass.open @06:07:20.09 Intake.close @06:07:20.80 Toroid.in :hold @06:07:20.86 Toroid.seek :crack @06:07:22.36 Toroid.seek :cartridge @06:07:23.45 Toroid.in :default @06:07:23.51 Toroid.seek :clear @06:07:25.48 Exhaust.close @06:07:25.52 Bypass.close @06:07:26.25 Verifying cartridge holds at least 0.65..0.85 of 35.3psia @06:07:26.27 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @06:07:30.90 Sampling 1000.0ml @06:07:30.92 SamplePump.setPosition! 0ml @06:07:30.98 Exhaust.open @06:07:31.75 Intake.open @06:07:32.53 Pressure.in :default @06:07:32.59 Pressure.hold @06:07:32.71 Sea 14.1m deep (35.6psia) @06:07:32.85 SamplePump.in :sample @06:07:32.92 SamplePump.seek 1000ml,1:23:48 @06:07:35.57 Cartridge 22 filtering 1/1000ml at 0.41ml/s with 24.0psi vacuum 13.5m deep (34.6psia) @06:08:35.56 Cartridge 22 filtering 26/1000ml at 0.38ml/s with 24.8psi vacuum 12.8m deep (33.6psia) @06:09:35.57 Cartridge 22 filtering 49/1000ml at 0.38ml/s with 26.5psi vacuum 14.5m deep (36.1psia) @06:10:35.56 Cartridge 22 filtering 71/1000ml at 0.37ml/s with 25.7psi vacuum 12.8m deep (33.6psia) @06:11:35.57 Cartridge 22 filtering 93/1000ml at 0.38ml/s with 25.6psi vacuum 12.1m deep (32.6psia) @06:12:35.56 Cartridge 22 filtering 114/1000ml at 0.36ml/s with 21.2psi vacuum 14.0m deep (35.4psia) @06:13:35.57 Cartridge 22 filtering 134/1000ml at 0.35ml/s with 22.4psi vacuum 13.0m deep (34.0psia) @06:14:35.56 Cartridge 22 filtering 154/1000ml at 0.33ml/s with 24.7psi vacuum 12.7m deep (33.4psia) @06:15:35.57 Cartridge 22 filtering 173/1000ml at 0.31ml/s with 22.7psi vacuum 12.5m deep (33.1psia) @06:16:35.56 Cartridge 22 filtering 192/1000ml at 0.29ml/s with 23.4psi vacuum 12.0m deep (32.5psia) @06:17:35.57 Cartridge 22 filtering 210/1000ml at 0.29ml/s with 23.3psi vacuum 12.9m deep (33.8psia) @06:18:35.56 Cartridge 22 filtering 227/1000ml at 0.28ml/s with 22.6psi vacuum 11.5m deep (31.6psia) @06:19:35.57 Cartridge 22 filtering 245/1000ml at 0.27ml/s with 25.2psi vacuum 11.5m deep (31.7psia) @06:20:35.56 Cartridge 22 filtering 262/1000ml at 0.26ml/s with 23.9psi vacuum 11.9m deep (32.2psia) @06:21:35.57 Cartridge 22 filtering 278/1000ml at 0.26ml/s with 22.7psi vacuum 11.4m deep (31.6psia) @06:22:35.56 Cartridge 22 filtering 294/1000ml at 0.26ml/s with 22.9psi vacuum 11.5m deep (31.7psia) @06:23:35.57 Cartridge 22 filtering 310/1000ml at 0.25ml/s with 26.2psi vacuum 11.6m deep (31.8psia) @06:24:35.56 Cartridge 22 filtering 325/1000ml at 0.25ml/s with 25.0psi vacuum 11.3m deep (31.4psia) @06:25:35.57 Cartridge 22 filtering 340/1000ml at 0.25ml/s with 23.5psi vacuum 10.6m deep (30.4psia) @06:26:35.56 Cartridge 22 filtering 355/1000ml at 0.23ml/s with 24.0psi vacuum 11.8m deep (32.1psia) @06:27:35.57 Cartridge 22 filtering 369/1000ml at 0.24ml/s with 22.7psi vacuum 11.2m deep (31.2psia) @06:28:35.56 Cartridge 22 filtering 383/1000ml at 0.22ml/s with 24.4psi vacuum 11.1m deep (31.1psia) @06:29:35.57 Cartridge 22 filtering 396/1000ml at 0.23ml/s with 24.2psi vacuum 11.0m deep (30.9psia) @06:30:35.56 Cartridge 22 filtering 410/1000ml at 0.21ml/s with 22.7psi vacuum 11.5m deep (31.7psia) @06:31:35.58 Cartridge 22 filtering 423/1000ml at 0.20ml/s with 24.2psi vacuum 10.9m deep (30.7psia) @06:32:35.56 Cartridge 22 filtering 436/1000ml at 0.21ml/s with 23.4psi vacuum 12.5m deep (33.2psia) @06:33:35.57 Cartridge 22 filtering 449/1000ml at 0.20ml/s with 25.7psi vacuum 10.6m deep (30.4psia) @06:34:35.56 Cartridge 22 filtering 461/1000ml at 0.20ml/s with 24.6psi vacuum 12.1m deep (32.5psia) @06:35:35.57 Cartridge 22 filtering 473/1000ml at 0.22ml/s with 26.7psi vacuum 11.7m deep (32.0psia) @06:36:35.56 Cartridge 22 filtering 486/1000ml at 0.20ml/s with 26.0psi vacuum 11.0m deep (31.0psia) @06:37:35.57 Cartridge 22 filtering 498/1000ml at 0.20ml/s with 22.5psi vacuum 11.1m deep (31.1psia) @06:38:12.37 Flow averaged 0.27ml/s over 30:40 @06:38:12.40 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi @06:38:13.40 Relieved excess vacuum in 1 second @06:38:13.43 SamplePump.seek 1000ml,41:42 @06:38:35.56 Cartridge 22 filtering 510/1000ml at 0.20ml/s with 26.4psi vacuum 11.3m deep (31.4psia) @06:39:35.57 Cartridge 22 filtering 522/1000ml at 0.20ml/s with 27.4psi vacuum 11.6m deep (31.9psia) @06:39:47.51 Flow averaged 0.20ml/s over 1:35 @06:39:47.54 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @06:39:48.12 Relieved excess vacuum in .6 seconds @06:39:48.14 SamplePump.seek 1000ml,40:07 @06:40:30.31 Flow averaged 0.20ml/s over 43 seconds @06:40:30.34 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @06:40:30.91 Relieved excess vacuum in .6 seconds @06:40:30.94 SamplePump.seek 1000ml,39:25 @06:40:35.57 Cartridge 22 filtering 534/1000ml at 0.20ml/s with 27.0psi vacuum 10.4m deep (30.0psia) @06:40:41.16 Flow averaged 0.20ml/s over 11 seconds @06:40:41.18 Intake.close @06:40:41.22 Exhaust.close @06:40:41.42 Toroid.in :hold @06:40:41.49 Toroid.seek :bypass @06:40:43.11 Toroid.in :default @06:40:43.17 Toroid.seek :clear @06:40:44.77 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @06:40:44.79 Pressure.stop @06:40:44.98 Sampled 535.0ml @06:40:47.06 -> Cmd.startProcessing @06:40:47.46 Plunge.seek :reagent @06:40:48.27 Sample loop closed. Ready to Deliver Reagents @06:40:48.28 Archive.seek :sealed @06:40:52.23 Archive.seek :evac @06:41:06.26 delay 1:00 @06:42:06.28 Archive.seek :clear @06:42:23.76 RNAlater soak for 10:00 @06:52:23.78 Archive.seek :tVent @06:52:26.22 delay 1 second @06:52:27.24 Archive.seek :evac @06:52:42.73 delay 10 seconds @06:52:52.76 Sample preserved and evacuated @06:52:53.87 -> Cmd.stop @06:52:54.01 Carousel.seek 22 @06:52:54.20 Plunge.seek :locked @06:52:55.11 Archive.in :unlocking @06:52:55.24 Archive.seek :clear @06:52:55.28 Air.in :unlocking @06:52:55.42 Air.seek :clear @06:52:55.51 Lysis1.in :unlocking @06:52:55.64 Lysis1.seek :clear @06:52:59.40 Gate.power :motor,:OFF @06:52:59.42 Gate.power :core,:OFF @06:52:59.44 Gate.power :analytic1,:OFF @06:52:59.47 Gate.power :analytic2,:OFF @06:52:59.49 Gate.power :analytic3,:OFF @06:52:59.51 Gate.power :main,:OFF @06:52:59.53 Safely stopped and ready to power off ##-- file closed 2022-06-06T14:04:46.346Z ##-- file opened 2022-06-06T14:04:46.348Z @07:04:47.11 -> showStatus @07:04:49.14 -> Cmd.loadCartridge -10 @07:04:49.20 Gate.power :main,:ON,limit to 5000mA @07:04:49.22 Gate.power :analytic1,:ON,limit to 5000mA @07:04:49.24 Gate.power :analytic2,:ON,limit to 5000mA @07:04:49.27 Gate.power :analytic3,:ON,limit to 5000mA @07:04:49.41 Powering core bus @07:04:51.68 SPR Module 1, FW Rev. B, FPGA Rev. C @07:04:52.13 Archive.configure :default @07:04:52.25 Air.configure :default @07:04:52.36 Lysis1.configure :default @07:04:52.46 Lysis2.configure :default @07:04:52.59 Plunge.configure :default @07:04:52.72 SamplePump.configure :default @07:04:52.85 Toroid.configure :default @07:04:52.97 Carousel.configure :default @07:04:53.10 Pressure.configure :default @07:04:53.23 ExtraPump.configure :default @07:04:53.33 PinPort.configure :default @07:04:53.39 All elf controllers running firmware version 4.79 @07:04:53.42 Gate.power :motor,:ON,limit to 5000mA @07:04:53.64 Archive.in :default @07:04:53.90 Archive.home.jog 0 @07:04:54.11 Toroid.in :default @07:04:54.17 Plunge.in :default @07:04:54.21 Lysis1.in :default @07:04:54.26 Air.in :default @07:04:54.32 Lysis2.in :default @07:04:54.38 PinPort.in :default @07:04:54.50 Toroid.home.jog 0 @07:04:54.58 Plunge.home.jog 0 @07:04:54.68 Lysis1.home.jog 0 @07:04:54.71 Air.home.jog 0 @07:04:54.75 PinPort.home.jog 0 @07:04:54.77 Lysis2.home.jog 0 @07:04:55.19 Archive.in :homing @07:04:55.48 Toroid.in :homing @07:04:55.52 Plunge.in :homing @07:04:55.55 Archive.jog 1388 @07:04:55.60 PinPort.in :homing @07:04:55.63 Lysis1.in :homing @07:04:55.66 Air.in :homing @07:04:55.73 Lysis2.in :homing @07:04:56.14 Plunge.jog 1555 @07:04:56.24 PinPort.jog -108 @07:04:56.36 Toroid.jog 632 @07:04:56.44 Lysis1.jog 1378 @07:04:56.48 Air.jog 1388 @07:04:56.68 Lysis2.jog 1393 @07:04:57.22 Archive.setRawPosition! 50 @07:04:57.26 PinPort.setRawPosition! 0 @07:04:57.33 Archive.seek :home @07:04:57.37 PinPort.seek :home @07:04:57.52 PinPort.in :default @07:04:57.66 Archive.in :default @07:04:57.81 Plunge.setRawPosition! 50 @07:04:57.86 Plunge.seek :home @07:04:58.03 Air.setRawPosition! 50 @07:04:58.08 Air.seek :home @07:04:58.19 Plunge.in :default @07:04:58.22 Lysis1.setRawPosition! 50 @07:04:58.31 Lysis1.seek :home @07:04:58.41 Lysis2.setRawPosition! 50 @07:04:58.44 Air.in :default @07:04:58.51 Lysis2.seek :home @07:04:58.63 Lysis1.in :default @07:04:58.82 Lysis2.in :default @07:04:58.95 Toroid.setRawPosition! 0 @07:04:59.00 Toroid.seek :home @07:04:59.49 Toroid.in :default @07:04:59.63 Toroid.seek :clear @07:05:01.24 Carousel.in :default @07:05:01.30 Carousel.home.jog 0 @07:05:01.78 Carousel.in :default @07:05:01.84 Carousel.seek 22 @07:05:01.99 Selecting Cartridge 21 @07:05:02.26 Carousel.seek 21 @07:05:02.74 Lysis1.in :fast @07:05:02.81 Lysis1.seek :locked @07:05:05.43 Plunge.seek :locked @07:05:05.57 Air.in :fast @07:05:05.63 Air.seek :locked @07:05:08.09 Powering cartridge bus @07:05:10.69 Gate.setIR 1750,5000,0,6 @07:05:10.82 Gate.setIR 0,5000,0,6 @07:05:10.88 PuckHeater.configure :default @07:05:10.95 Gate.power :heat,:ON,limit to 5000mA @07:05:11.62 Mounted lysis Cartridge board#Q05031 @07:05:11.66 Using default puck heat loss curve for HeatingAccelInCarousel @07:05:29.54 -> Cmd.startFiltering @07:05:29.78 92.07 Preparing to filter cartridge 21 @07:05:30.07 Priming sample loop w/100ml, bypass w/10ml @07:05:30.08 SamplePump.setPosition! 0ml @07:05:30.13 Bypass.open @07:05:31.56 Intake.open @07:05:32.28 Sea 15.1m deep (37.0psia) @07:05:32.30 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:05:34.88 Vacuum tare is 54% of full scale @07:05:35.02 Intake.close @07:05:35.07 Bypass.close @07:05:35.77 Verifying toroid holds at least 0.6..0.85 of 37.0psia @07:05:35.80 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @07:05:40.36 Bypass.open @07:05:41.03 Intake.open @07:05:42.46 Pressure.in :default @07:05:42.52 Pressure.hold @07:05:42.71 SamplePump.in :prime @07:05:42.77 SamplePump.seek 10ml,59 seconds @07:05:51.39 Pressure.stop @07:05:51.44 SamplePump.setPosition! 0ml @07:05:51.50 Exhaust.open @07:05:53.00 Bypass.close @07:05:54.48 Pressure.in :default @07:05:54.55 Pressure.hold @07:05:54.66 Sea 14.9m deep (36.8psia) @07:05:54.80 SamplePump.in :prime @07:05:54.87 SamplePump.seek 100ml,5:16 @07:07:28.10 Pressure.stop @07:07:28.17 Bypass.open @07:07:28.83 Intake.close @07:07:29.58 Toroid.in :hold @07:07:29.64 Toroid.seek :crack @07:07:31.15 Toroid.seek :cartridge @07:07:32.24 Toroid.in :default @07:07:32.30 Toroid.seek :clear @07:07:34.27 Exhaust.close @07:07:34.33 Bypass.close @07:07:35.05 Verifying cartridge holds at least 0.65..0.85 of 35.8psia @07:07:35.06 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @07:07:39.69 Sampling 1000.0ml @07:07:39.71 SamplePump.setPosition! 0ml @07:07:39.76 Exhaust.open @07:07:40.48 Intake.open @07:07:41.30 Pressure.in :default @07:07:41.36 Pressure.hold @07:07:41.52 Sea 14.4m deep (36.0psia) @07:07:41.67 SamplePump.in :sample @07:07:41.74 SamplePump.seek 1000ml,1:23:48 @07:07:44.33 Cartridge 21 filtering 1/1000ml at 0.43ml/s with 24.4psi vacuum 12.6m deep (33.3psia) @07:08:44.32 Cartridge 21 filtering 27/1000ml at 0.40ml/s with 22.8psi vacuum 14.6m deep (36.2psia) @07:09:44.33 Cartridge 21 filtering 51/1000ml at 0.41ml/s with 25.9psi vacuum 14.4m deep (36.0psia) @07:10:44.32 Cartridge 21 filtering 75/1000ml at 0.41ml/s with 24.2psi vacuum 14.6m deep (36.3psia) @07:11:44.33 Cartridge 21 filtering 97/1000ml at 0.39ml/s with 25.8psi vacuum 13.6m deep (34.9psia) @07:12:44.32 Cartridge 21 filtering 119/1000ml at 0.36ml/s with 20.0psi vacuum 15.2m deep (37.2psia) @07:13:44.33 Cartridge 21 filtering 140/1000ml at 0.34ml/s with 22.1psi vacuum 14.4m deep (36.0psia) @07:14:44.32 Cartridge 21 filtering 161/1000ml at 0.31ml/s with 24.5psi vacuum 13.1m deep (34.0psia) @07:15:44.32 Cartridge 21 filtering 181/1000ml at 0.32ml/s with 23.0psi vacuum 13.0m deep (33.9psia) @07:16:44.32 Cartridge 21 filtering 201/1000ml at 0.32ml/s with 25.8psi vacuum 13.3m deep (34.4psia) @07:17:44.33 Cartridge 21 filtering 220/1000ml at 0.30ml/s with 24.8psi vacuum 14.0m deep (35.4psia) @07:18:44.33 Cartridge 21 filtering 238/1000ml at 0.30ml/s with 23.5psi vacuum 13.8m deep (35.1psia) @07:19:44.33 Cartridge 21 filtering 256/1000ml at 0.29ml/s with 23.1psi vacuum 14.0m deep (35.4psia) @07:20:44.32 Cartridge 21 filtering 274/1000ml at 0.29ml/s with 24.9psi vacuum 14.9m deep (36.7psia) @07:21:44.33 Cartridge 21 filtering 291/1000ml at 0.29ml/s with 25.2psi vacuum 14.4m deep (36.0psia) @07:22:44.32 Cartridge 21 filtering 308/1000ml at 0.27ml/s with 22.5psi vacuum 14.3m deep (35.9psia) @07:23:44.35 Cartridge 21 filtering 324/1000ml at 0.27ml/s with 25.3psi vacuum 14.0m deep (35.4psia) @07:24:44.32 Cartridge 21 filtering 340/1000ml at 0.24ml/s with 25.6psi vacuum 13.3m deep (34.3psia) @07:25:44.33 Cartridge 21 filtering 355/1000ml at 0.24ml/s with 23.3psi vacuum 13.5m deep (34.7psia) @07:26:44.32 Cartridge 21 filtering 370/1000ml at 0.24ml/s with 23.6psi vacuum 14.1m deep (35.5psia) @07:27:44.33 Cartridge 21 filtering 385/1000ml at 0.23ml/s with 25.2psi vacuum 14.0m deep (35.3psia) @07:28:44.32 Cartridge 21 filtering 399/1000ml at 0.24ml/s with 24.8psi vacuum 12.7m deep (33.5psia) @07:29:44.33 Cartridge 21 filtering 413/1000ml at 0.23ml/s with 24.1psi vacuum 13.8m deep (35.0psia) @07:30:44.32 Cartridge 21 filtering 427/1000ml at 0.21ml/s with 22.8psi vacuum 14.4m deep (35.9psia) @07:31:44.33 Cartridge 21 filtering 440/1000ml at 0.22ml/s with 24.5psi vacuum 13.3m deep (34.3psia) @07:32:44.33 Cartridge 21 filtering 453/1000ml at 0.22ml/s with 25.0psi vacuum 13.8m deep (35.2psia) @07:33:44.33 Cartridge 21 filtering 466/1000ml at 0.20ml/s with 25.1psi vacuum 13.2m deep (34.2psia) @07:34:44.33 Cartridge 21 filtering 479/1000ml at 0.22ml/s with 22.0psi vacuum 14.2m deep (35.6psia) @07:35:44.75 Cartridge 21 filtering 491/1000ml at 0.21ml/s with 21.9psi vacuum 14.7m deep (36.5psia) @07:36:44.33 Cartridge 21 filtering 504/1000ml at 0.20ml/s with 25.2psi vacuum 15.2m deep (37.1psia) @07:37:44.38 Cartridge 21 filtering 516/1000ml at 0.20ml/s with 25.5psi vacuum 14.0m deep (35.4psia) @07:38:44.33 Cartridge 21 filtering 528/1000ml at 0.21ml/s with 23.9psi vacuum 12.9m deep (33.8psia) @07:39:11.27 Flow averaged 0.28ml/s over 31:30 @07:39:11.32 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @07:39:11.89 Relieved excess vacuum in .6 seconds @07:39:11.91 SamplePump.seek 1000ml,39:21 @07:39:44.33 Cartridge 21 filtering 540/1000ml at 0.20ml/s with 27.4psi vacuum 14.9m deep (36.7psia) @07:39:57.28 Flow averaged 0.20ml/s over 46 seconds @07:39:57.32 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi @07:39:57.89 Relieved excess vacuum in .6 seconds @07:39:57.91 SamplePump.seek 1000ml,38:35 @07:40:44.33 Cartridge 21 filtering 552/1000ml at 0.20ml/s with 26.5psi vacuum 12.7m deep (33.5psia) @07:40:51.37 Flow averaged 0.20ml/s over 54 seconds @07:40:51.42 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi @07:40:51.98 Relieved excess vacuum in .7 seconds @07:40:52.01 SamplePump.seek 1000ml,37:41 @07:41:34.19 Flow averaged 0.20ml/s over 43 seconds @07:41:34.22 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @07:41:34.80 Relieved excess vacuum in .6 seconds @07:41:34.82 SamplePump.seek 1000ml,36:58 @07:41:38.21 Flow estimate reduced to 0.16ml/s @07:41:38.23 Intake.close @07:41:38.29 Exhaust.close @07:41:38.48 Toroid.in :hold @07:41:38.55 Toroid.seek :bypass @07:41:40.22 Toroid.in :default @07:41:40.28 Toroid.seek :clear @07:41:41.89 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @07:41:41.91 Pressure.stop @07:41:42.10 Sampled 562.9ml @07:41:44.33 -> Cmd.startProcessing @07:41:45.76 SPR.command \"read_sequences\" @07:41:45.82 SPR.command \"stats_on\" @07:41:45.92 SPR.command \"start 0 1\" @07:41:46.67 SPR.start /var/log/esp/spr22jun0607h.spr.bz2 @07:41:48.58 Handoff.select :waste @07:41:55.25 Syringe tare is 19% of full scale @07:41:55.28 PinPort.in :locking @07:41:55.35 PinPort.seek :locked @07:41:55.83 Archive.in :fast @07:41:55.87 Lysis2.in :fast @07:41:55.95 Lysis1.seek 1350uL @07:41:56.03 Archive.seek :full @07:41:56.08 Lysis2.seek :full @07:41:58.11 Plunge.seek :reagent @07:41:58.98 Evacuating puck with filter bubble point of 50psi @07:41:59.01 PinPort.seek :locked @07:41:59.10 Handoff.select :waste @07:42:05.67 Air syringe pumping up to 1 stroke to reach 8psi @07:42:05.69 Air stroke #1 of 1 @07:42:05.78 Air.in :pressurize @07:42:05.85 Air.seek :pressurized @07:42:17.45 0.3psi after pressurize @07:42:17.47 Air.in :compress @07:42:17.55 Air.seek :evac @07:42:21.64 0.7psi after compress @07:42:34.22 0.0psi after compress (stable) @07:42:34.23 Air.in :default @07:42:34.30 Air.hold @07:42:34.51 at -0.0psi Opened to waste @07:42:34.52 Handoff.select :blocked @07:42:34.65 Archive syringe pumping up to 8 strokes to reach 50psi @07:42:34.67 Archive stroke #1 of 8 @07:42:34.75 Archive.in :pressurize @07:42:34.82 Archive.seek :pressurized @07:42:47.70 8.8psi after pressurize @07:42:47.74 Archive.in :compress @07:42:47.81 Archive.seek :empty @07:43:02.50 31.1psi after compress @07:43:21.57 36.2psi after compress (stable) @07:43:21.59 Archive.in :gulp @07:43:21.67 Archive.seek :topVent @07:43:28.85 36.9psi after gulp @07:43:41.42 36.9psi after gulp (stable) @07:43:41.44 Archive stroke #2 of 8 @07:43:41.53 Archive.in :pressurize @07:43:41.62 Archive.seek :pressurized @07:43:54.49 36.5psi after pressurize @07:43:54.50 Archive.in :compress @07:43:54.58 Archive.seek :empty @07:44:07.64 Reached 50.2 of 50.0psi after pumping 3425uL (1.98 strokes) @07:44:07.65 Archive.in :default @07:44:07.72 Archive.hold @07:44:07.79 Handoff.select :waste @07:44:17.47 delay 1:00 @07:45:17.49 Handoff.select :blocked @07:45:43.22 Pressure too High, open to waste again (8.1psi) @07:45:43.23 Handoff.select :waste @07:45:43.36 delay 1:00 @07:46:43.38 Handoff.select :blocked @07:47:25.55 Handoff.select :waste @07:47:25.66 7.6psi at evacEnd @07:47:25.67 Handoff.select :blocked @07:47:25.81 Archive.in :fast @07:47:25.87 Archive.seek :topVent @07:47:46.40 Stable @5.9psi after Handoff blocked @07:47:46.43 Archive syringe pumping up to 8 strokes to reach 36psi @07:47:46.44 Archive stroke #1 of 8 @07:47:46.55 Archive.in :pressurize @07:47:46.65 Archive.seek :pressurized @07:47:59.50 7.6psi after pressurize @07:47:59.52 Archive.in :compress @07:47:59.60 Archive.seek :empty @07:48:14.27 11.7psi after compress @07:48:58.38 24.4psi after compress (stable) @07:48:58.40 Archive.in :gulp @07:48:58.51 Archive.seek :topVent @07:49:05.67 25.6psi after gulp @07:49:18.24 26.5psi after gulp (stable) @07:49:18.25 Archive stroke #2 of 8 @07:49:18.37 Archive.in :pressurize @07:49:18.44 Archive.seek :pressurized @07:49:31.29 27.6psi after pressurize @07:49:31.31 Archive.in :compress @07:49:31.38 Archive.seek :empty @07:49:46.06 30.1psi after compress @07:50:04.60 Reached 36.0 of 35.9psi after pumping 3460uL (2.00 strokes) @07:50:04.62 Archive.in :default @07:50:04.72 Archive.hold @07:50:04.79 Handoff.select :waste @07:50:16.01 delay 1:00 @07:51:16.03 Handoff.select :blocked @07:51:40.71 Handoff.select :waste @07:51:40.83 3.4psi at evacEnd @07:51:40.85 Air.seek :fullVent @07:51:42.27 Archive.in :fast @07:51:42.34 Archive.seek :topVent @07:51:49.44 Seawater evacuated, Presure above/below filter released. Lysis begins @07:51:49.53 Lysis1.in :slow @07:51:49.59 Lysis1.seek 1050uL @07:52:19.61 delay 5 seconds @07:52:24.63 Handoff.select :blocked @07:52:24.82 Lysis1.seek 450uL @07:53:24.77 delay 3 seconds @07:53:27.84 Air.in mix with 1.40048 parts Lysis1 @07:53:28.37 Air.seek :evac @07:53:28.45 Lysis1.seek 300uL @07:53:43.52 Lysis1.seek :empty @07:54:13.60 Lysis buffer delivered @15.8psi @07:54:13.61 Polling Can every 2:00 @07:54:14.16 PuckHeater.configure :default #ambient 15.9C @07:54:14.20 PuckHeater.seek 80.1C @07:55:07.77 PuckHeater.hold 2:00,80.1C @07:57:07.65 Polling Can every 2:00 @07:57:08.19 PuckHeater.configure :default #ambient 15.9C @07:57:08.24 PuckHeater.hold 30 seconds,79C @07:57:38.35 Polling Can every 2:00 @07:57:38.89 PuckHeater.configure :default #ambient 15.9C @07:57:38.94 PuckHeater.hold 30 seconds,78.5C @07:58:09.06 Polling Can every 2:00 @07:58:09.60 PuckHeater.configure :default #ambient 15.9C @07:58:09.66 PuckHeater.hold 10:00,78C @08:08:08.62 Lysis complete. Syringe pressure @17.7psi @08:08:08.63 Handoff.select :blocked @08:08:08.68 Archive syringe pumping up to 5 strokes to reach 40psi @08:08:08.69 Archive stroke #1 of 5 @08:08:08.79 Archive.in :pressurize @08:08:08.86 Archive.seek :pressurized @08:08:21.71 17.5psi after pressurize @08:08:21.73 Archive.in :compress @08:08:21.82 Archive.seek :empty @08:08:28.86 Reached 40.4 of 40.0psi after pumping 1565uL (0.90 strokes) @08:08:28.88 Archive.in :default @08:08:28.95 Archive.hold @08:08:29.02 delay 2 seconds @08:08:31.04 Archive.in :fast @08:08:31.10 Archive.seek :full @08:08:37.69 Lysis1.seek 138uL @08:08:51.55 delay 1 second @08:08:52.57 Archive.seek :full @08:08:52.63 Lysis1.seek :empty @08:09:06.59 Initial air pressure @42.2psi @08:09:06.67 Lysis1.in :extract @08:09:06.77 Lysis1.seek 600uL,nil @08:09:09.02 Archive syringe pumping up to 6 strokes to reach 25psi @08:09:09.03 Archive stroke #1 of 6 @08:09:09.21 Archive.in :pressurize @08:09:09.27 Archive.seek :pressurized @08:09:22.12 15.8psi after pressurize @08:09:22.14 Archive.in :compress @08:09:22.21 Archive.seek :empty @08:09:35.69 Archive.in :default @08:09:35.74 Archive.hold @08:09:35.89 Initial 600uL lysate @17.3psi @08:09:35.91 Handoff.select :blocked @08:09:36.01 Initial air pressure @18.1psi @08:09:36.08 Lysis1.seek 825uL,nil @08:09:37.27 Archive syringe pumping up to 6 strokes to reach 17psi @08:09:37.28 Archive stroke #1 of 6 @08:09:37.38 Archive.in :compress @08:09:37.45 Archive.seek :empty @08:09:38.16 Reached 17.0 of 17.0psi after pumping 1718uL (0.99 strokes) @08:09:38.17 Archive.in :default @08:09:38.24 Archive.hold @08:09:38.98 Archive syringe pumping up to 6 strokes to reach 17psi @08:09:38.99 Archive stroke #1 of 6 @08:09:39.11 Archive.in :compress @08:09:39.17 Archive.seek :empty @08:09:39.95 16.2psi after compress @08:09:52.52 15.8psi after compress (stable) @08:09:57.68 Archive.in :gulp @08:09:57.74 Archive.seek :topVent @08:10:04.93 15.8psi after gulp @08:10:17.57 15.7psi after gulp (stable) @08:10:17.58 Archive stroke #2 of 6 @08:10:17.75 Archive.in :pressurize @08:10:17.82 Archive.seek :pressurized @08:10:30.68 15.5psi after pressurize @08:10:30.70 Archive.in :compress @08:10:30.76 Archive.seek :empty @08:10:45.43 16.1psi after compress @08:10:52.01 Archive.in :default @08:10:52.07 Archive.hold @08:10:52.28 Recovered 825uL lysate @16.0psi @08:10:52.29 Air.in :halfSpeed @08:10:52.37 Air.seek :fullVent @08:10:54.58 Puck Top Vented, bottom @1.7psi @08:10:54.59 Handoff.select :waste @08:10:54.71 delay 5 seconds @08:10:59.81 Handoff open to waste, bottom @0.1psi @08:10:59.84 Air.in :default @08:10:59.91 Air.seek :evac @08:11:01.33 Lysis1.in :DAmix @08:11:01.45 Lysis2.in :DAmix @08:11:01.99 Lysis1.seek 575uL @08:11:02.05 Lysis2.seek 1480uL @08:11:37.07 delay 5 seconds @08:11:42.09 Handoff.select SPR @08:11:42.20 SPR.command \"start 2 14\" @08:11:42.82 SPR stabilized @08:11:42.96 delay 10 seconds @08:11:52.98 SPR.command \"start 1 0\" @08:11:56.18 Waiting up to 4:00 for SPR to enter state 1 @08:13:58.56 SPR.command \"hold_on\" @08:13:59.97 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:14:00.45 Lysis1.seek 275uL @08:14:00.50 Lysis2.seek 1180uL @08:14:42.49 delay 15 seconds @08:14:57.50 SPR.command \"hold_off\" @08:15:00.62 Waiting up to 38:00 for SPR to enter state 7 @08:35:50.45 SPRsummary:-8.54,130.504,136.052,173.453 after 3402s @08:42:27.68 SPR analysis completed @08:42:34.05 Ended log spr22jun0607h.spr.bz2 @08:42:34.07 SPR.command \"stats_off\" @08:42:35.44 -> Cmd.stop @08:42:35.54 Handoff.select :close @08:42:35.67 Gate.power :heat,:OFF @08:42:35.69 Gate.power :cartridge,:OFF @08:42:35.77 Carousel.seek 21 @08:42:35.96 Plunge.seek :locked @08:42:36.82 PinPort.in :unlocking @08:42:36.88 PinPort.seek :clear @08:42:37.43 Archive.in :unlocking @08:42:37.56 Archive.seek :clear @08:42:37.67 Air.in :unlocking @08:42:37.77 Lysis1.in :unlocking @08:42:37.81 Air.seek :clear @08:42:37.92 Lysis1.seek :clear @08:42:38.07 Lysis2.in :unlocking @08:42:38.14 Lysis2.seek :clear @08:42:41.74 Gate.power :motor,:OFF @08:42:41.76 Gate.power :core,:OFF @08:42:41.78 Gate.power :analytic1,:OFF @08:42:41.81 Gate.power :analytic2,:OFF @08:42:41.83 Gate.power :analytic3,:OFF @08:42:41.86 Gate.power :main,:OFF @08:42:41.88 Safely stopped and ready to power off ##-- file closed 2022-06-06T19:34:04.921Z ##-- file opened 2022-06-06T19:34:04.924Z @12:34:05.57 -> showStatus @12:34:07.59 -> Cmd.loadCartridge -6 @12:34:07.65 Gate.power :main,:ON,limit to 5000mA @12:34:07.67 Gate.power :analytic1,:ON,limit to 5000mA @12:34:07.69 Gate.power :analytic2,:ON,limit to 5000mA @12:34:07.71 Gate.power :analytic3,:ON,limit to 5000mA @12:34:07.85 Powering core bus @12:34:10.11 SPR Module 1, FW Rev. B, FPGA Rev. C @12:34:10.56 Archive.configure :default @12:34:10.69 Air.configure :default @12:34:10.80 Lysis1.configure :default @12:34:10.90 Lysis2.configure :default @12:34:11.03 Plunge.configure :default @12:34:11.26 SamplePump.configure :default @12:34:11.52 Toroid.configure :default @12:34:11.79 Carousel.configure :default @12:34:12.06 Pressure.configure :default @12:34:12.38 ExtraPump.configure :default @12:34:12.66 PinPort.configure :default @12:34:12.72 All elf controllers running firmware version 4.79 @12:34:12.75 Gate.power :motor,:ON,limit to 5000mA @12:34:12.87 Archive.in :default @12:34:13.17 Archive.home.jog 0 @12:34:13.38 Toroid.in :default @12:34:13.42 Plunge.in :default @12:34:13.45 Lysis1.in :default @12:34:13.50 Air.in :default @12:34:13.53 PinPort.in :default @12:34:13.57 Lysis2.in :default @12:34:13.80 Toroid.home.jog 0 @12:34:13.83 Plunge.home.jog 0 @12:34:13.86 Air.home.jog 0 @12:34:13.89 PinPort.home.jog 0 @12:34:13.91 Lysis1.home.jog 0 @12:34:13.93 Lysis2.home.jog 0 @12:34:14.33 Archive.in :homing @12:34:14.52 Toroid.in :homing @12:34:14.55 Plunge.in :homing @12:34:14.61 Archive.jog 1386 @12:34:14.66 PinPort.in :homing @12:34:14.70 Air.in :homing @12:34:14.90 Plunge.jog 1555 @12:34:14.92 Toroid.jog 638 @12:34:15.00 PinPort.jog -107 @12:34:15.02 Air.jog 1392 @12:34:15.24 Lysis2.in :homing @12:34:15.27 Lysis1.in :homing @12:34:15.37 Lysis2.jog 1391 @12:34:15.40 Lysis1.jog 1377 @12:34:15.51 PinPort.setRawPosition! 0 @12:34:15.55 PinPort.seek :home @12:34:15.64 PinPort.in :default @12:34:15.94 Archive.setRawPosition! 50 @12:34:15.99 Archive.seek :home @12:34:16.28 Plunge.setRawPosition! 50 @12:34:16.33 Plunge.seek :home @12:34:16.41 Air.setRawPosition! 50 @12:34:16.43 Archive.in :default @12:34:16.52 Air.seek :home @12:34:16.65 Plunge.in :default @12:34:16.68 Lysis2.setRawPosition! 50 @12:34:16.70 Lysis1.setRawPosition! 50 @12:34:16.80 Lysis2.seek :home @12:34:16.88 Lysis1.seek :home @12:34:17.01 Air.in :default @12:34:17.16 Lysis2.in :default @12:34:17.28 Toroid.setRawPosition! 0 @12:34:17.29 Lysis1.in :default @12:34:17.67 Toroid.seek :home @12:34:17.86 Toroid.in :default @12:34:18.13 Toroid.seek :clear @12:34:19.75 Carousel.in :default @12:34:19.81 Carousel.home.jog 0 @12:34:20.31 Carousel.in :default @12:34:20.38 Carousel.seek 21 @12:34:20.52 Selecting Cartridge 20 @12:34:20.79 Carousel.seek 20 @12:34:21.27 Lysis1.in :fast @12:34:21.33 Lysis1.seek :locked @12:34:23.95 Plunge.seek :locked @12:34:24.08 Air.in :fast @12:34:24.14 Air.seek :locked @12:34:26.60 Powering cartridge bus @12:34:29.20 Gate.setIR 1750,5000,0,6 @12:34:29.32 Gate.setIR 0,5000,0,6 @12:34:29.37 Gate.power :cartridge,:OFF @12:34:29.91 Mounted archive Cartridge @12:34:47.18 -> Cmd.startFiltering @12:34:47.42 Preparing to filter cartridge 20 @12:34:47.70 Priming sample loop w/100ml, bypass w/10ml @12:34:47.72 SamplePump.setPosition! 0ml @12:34:47.77 Bypass.open @12:34:49.21 Intake.open @12:34:49.97 Sea 15.1m deep (37.0psia) @12:34:49.99 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:34:52.56 Vacuum tare is 54% of full scale @12:34:52.70 Intake.close @12:34:52.74 Bypass.close @12:34:53.49 Verifying toroid holds at least 0.6..0.85 of 37.0psia @12:34:53.52 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @12:34:58.08 Bypass.open @12:34:58.81 Intake.open @12:35:00.22 Pressure.in :default @12:35:00.28 Pressure.hold @12:35:00.46 SamplePump.in :prime @12:35:00.53 SamplePump.seek 10ml,59 seconds @12:35:09.14 Pressure.stop @12:35:09.20 SamplePump.setPosition! 0ml @12:35:09.25 Exhaust.open @12:35:10.73 Bypass.close @12:35:12.24 Pressure.in :default @12:35:12.31 Pressure.hold @12:35:12.43 Sea 15.1m deep (37.0psia) @12:35:12.56 SamplePump.in :prime @12:35:12.63 SamplePump.seek 100ml,5:16 @12:36:46.08 Pressure.stop @12:36:46.14 Bypass.open @12:36:46.80 Intake.close @12:36:47.34 Toroid.in :hold @12:36:47.40 Toroid.seek :crack @12:36:48.91 Toroid.seek :cartridge @12:36:50.01 Toroid.in :default @12:36:50.07 Toroid.seek :clear @12:36:52.04 Exhaust.close @12:36:52.08 Bypass.close @12:36:52.83 Verifying cartridge holds at least 0.65..0.85 of 36.6psia @12:36:52.85 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @12:36:57.47 Sampling 1000.0ml @12:36:57.49 SamplePump.setPosition! 0ml @12:36:57.55 Exhaust.open @12:36:58.30 Intake.open @12:36:59.12 Pressure.in :default @12:36:59.19 Pressure.hold @12:36:59.31 Sea 14.9m deep (36.7psia) @12:36:59.46 SamplePump.in :sample @12:36:59.52 SamplePump.seek 1000ml,1:23:48 @12:37:02.15 Cartridge 20 filtering 1/1000ml at 0.44ml/s with 26.6psi vacuum 14.7m deep (36.5psia) @12:38:02.15 Cartridge 20 filtering 26/1000ml at 0.38ml/s with 23.6psi vacuum 12.2m deep (32.7psia) @12:39:02.15 Cartridge 20 filtering 49/1000ml at 0.40ml/s with 22.4psi vacuum 12.9m deep (33.7psia) @12:40:02.15 Cartridge 20 filtering 72/1000ml at 0.37ml/s with 20.1psi vacuum 13.6m deep (34.8psia) @12:41:02.15 Cartridge 20 filtering 95/1000ml at 0.38ml/s with 24.5psi vacuum 11.8m deep (32.1psia) @12:42:02.15 Cartridge 20 filtering 117/1000ml at 0.34ml/s with 22.6psi vacuum 12.9m deep (33.7psia) @12:43:02.15 Cartridge 20 filtering 138/1000ml at 0.37ml/s with 22.6psi vacuum 11.6m deep (31.8psia) @12:44:02.16 Cartridge 20 filtering 159/1000ml at 0.33ml/s with 23.0psi vacuum 13.0m deep (33.9psia) @12:45:02.15 Cartridge 20 filtering 179/1000ml at 0.34ml/s with 25.9psi vacuum 12.1m deep (32.5psia) @12:46:02.14 Cartridge 20 filtering 200/1000ml at 0.32ml/s with 24.7psi vacuum 11.7m deep (32.0psia) @12:47:02.15 Cartridge 20 filtering 219/1000ml at 0.35ml/s with 22.9psi vacuum 13.1m deep (34.0psia) @12:48:02.14 Cartridge 20 filtering 239/1000ml at 0.30ml/s with 25.8psi vacuum 11.1m deep (31.1psia) @12:49:02.14 Cartridge 20 filtering 258/1000ml at 0.33ml/s with 21.5psi vacuum 12.6m deep (33.3psia) @12:50:02.14 Cartridge 20 filtering 277/1000ml at 0.29ml/s with 24.2psi vacuum 11.5m deep (31.7psia) @12:51:02.15 Cartridge 20 filtering 296/1000ml at 0.32ml/s with 23.6psi vacuum 14.2m deep (35.7psia) @12:52:02.14 Cartridge 20 filtering 314/1000ml at 0.31ml/s with 20.5psi vacuum 12.9m deep (33.7psia) @12:53:02.15 Cartridge 20 filtering 332/1000ml at 0.29ml/s with 23.5psi vacuum 12.6m deep (33.3psia) @12:54:02.15 Cartridge 20 filtering 350/1000ml at 0.28ml/s with 23.9psi vacuum 11.7m deep (32.0psia) @12:55:02.15 Cartridge 20 filtering 367/1000ml at 0.27ml/s with 26.5psi vacuum 9.9m deep (29.3psia) @12:56:02.14 Cartridge 20 filtering 384/1000ml at 0.29ml/s with 25.4psi vacuum 12.1m deep (32.5psia) @12:57:02.15 Cartridge 20 filtering 401/1000ml at 0.26ml/s with 24.5psi vacuum 11.1m deep (31.1psia) @12:58:02.14 Cartridge 20 filtering 417/1000ml at 0.26ml/s with 26.1psi vacuum 11.1m deep (31.1psia) @12:59:02.14 Cartridge 20 filtering 433/1000ml at 0.26ml/s with 23.0psi vacuum 11.9m deep (32.3psia) @13:00:02.14 Cartridge 20 filtering 449/1000ml at 0.25ml/s with 24.8psi vacuum 10.5m deep (30.2psia) @13:01:02.15 Cartridge 20 filtering 465/1000ml at 0.27ml/s with 21.2psi vacuum 12.6m deep (33.4psia) @13:02:02.14 Cartridge 20 filtering 480/1000ml at 0.25ml/s with 22.1psi vacuum 11.0m deep (31.0psia) @13:03:02.15 Cartridge 20 filtering 495/1000ml at 0.26ml/s with 22.2psi vacuum 10.5m deep (30.2psia) @13:04:02.15 Cartridge 20 filtering 510/1000ml at 0.27ml/s with 24.5psi vacuum 12.4m deep (33.1psia) @13:05:02.15 Cartridge 20 filtering 525/1000ml at 0.23ml/s with 24.0psi vacuum 12.4m deep (33.0psia) @13:06:02.62 Cartridge 20 filtering 539/1000ml at 0.26ml/s with 23.4psi vacuum 10.5m deep (30.1psia) @13:07:02.15 Cartridge 20 filtering 553/1000ml at 0.23ml/s with 23.4psi vacuum 11.9m deep (32.3psia) @13:08:02.15 Cartridge 20 filtering 567/1000ml at 0.21ml/s with 21.9psi vacuum 11.0m deep (30.9psia) @13:09:02.15 Cartridge 20 filtering 580/1000ml at 0.23ml/s with 22.7psi vacuum 10.6m deep (30.3psia) @13:10:02.15 Cartridge 20 filtering 594/1000ml at 0.21ml/s with 25.1psi vacuum 12.4m deep (33.0psia) @13:11:02.15 Cartridge 20 filtering 607/1000ml at 0.21ml/s with 24.5psi vacuum 10.7m deep (30.4psia) @13:12:02.15 Cartridge 20 filtering 620/1000ml at 0.20ml/s with 24.9psi vacuum 10.8m deep (30.7psia) @13:13:02.15 Cartridge 20 filtering 632/1000ml at 0.20ml/s with 26.4psi vacuum 10.6m deep (30.3psia) @13:14:02.15 Cartridge 20 filtering 645/1000ml at 0.20ml/s with 25.4psi vacuum 10.7m deep (30.4psia) @13:15:02.15 Cartridge 20 filtering 657/1000ml at 0.20ml/s with 26.4psi vacuum 11.7m deep (32.0psia) @13:16:02.15 Cartridge 20 filtering 670/1000ml at 0.21ml/s with 23.1psi vacuum 11.7m deep (31.9psia) @13:17:02.15 Cartridge 20 filtering 682/1000ml at 0.20ml/s with 25.8psi vacuum 11.6m deep (31.9psia) @13:18:02.15 Cartridge 20 filtering 694/1000ml at 0.20ml/s with 29.2psi vacuum 10.2m deep (29.8psia) @13:18:08.93 Flow averaged 0.28ml/s over 41:10 @13:18:08.97 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @13:18:09.54 Relieved excess vacuum in .6 seconds @13:18:09.56 SamplePump.seek 1000ml,25:52 @13:19:02.16 Cartridge 20 filtering 706/1000ml at 0.21ml/s with 24.2psi vacuum 12.3m deep (32.8psia) @13:19:20.98 Flow averaged 0.20ml/s over 1:12 @13:19:21.01 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi @13:19:21.58 Relieved excess vacuum in .6 seconds @13:19:21.61 SamplePump.seek 1000ml,24:40 @13:20:02.15 Cartridge 20 filtering 718/1000ml at 0.20ml/s with 27.9psi vacuum 10.8m deep (30.6psia) @13:20:05.60 Flow averaged 0.20ml/s over 44 seconds @13:20:05.63 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @13:20:06.20 Relieved excess vacuum in .6 seconds @13:20:06.23 SamplePump.seek 1000ml,23:56 @13:20:34.18 Flow averaged 0.20ml/s over 28 seconds @13:20:34.20 Intake.close @13:20:34.26 Exhaust.close @13:20:34.45 Toroid.in :hold @13:20:34.52 Toroid.seek :bypass @13:20:36.14 Toroid.in :default @13:20:36.21 Toroid.seek :clear @13:20:37.82 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @13:20:37.84 Pressure.stop @13:20:38.03 Sampled 724.4ml @13:20:40.09 -> Cmd.startProcessing @13:20:40.49 Plunge.seek :reagent @13:20:41.30 Sample loop closed. Ready to Deliver Reagents @13:20:41.31 Archive.seek :sealed @13:20:45.26 Archive.seek :evac @13:20:59.29 delay 1:00 @13:21:59.31 Archive.seek :clear @13:22:16.79 RNAlater soak for 10:00 @13:32:16.82 Archive.seek :tVent @13:32:19.27 delay 1 second @13:32:20.29 Archive.seek :evac @13:32:35.79 delay 10 seconds @13:32:45.81 Sample preserved and evacuated @13:32:46.90 -> Cmd.stop @13:32:47.05 Carousel.seek 20 @13:32:47.24 Plunge.seek :locked @13:32:48.16 Archive.in :unlocking @13:32:48.29 Archive.seek :clear @13:32:48.33 Air.in :unlocking @13:32:48.49 Air.seek :clear @13:32:48.60 Lysis1.in :unlocking @13:32:48.68 Lysis1.seek :clear @13:32:52.45 Gate.power :motor,:OFF @13:32:52.47 Gate.power :core,:OFF @13:32:52.55 Gate.power :analytic1,:OFF @13:32:52.56 Gate.power :analytic2,:OFF @13:32:52.59 Gate.power :analytic3,:OFF @13:32:52.61 Gate.power :main,:OFF @13:32:52.63 Safely stopped and ready to power off ##-- file closed 2022-06-06T20:45:15.002Z ##-- file opened 2022-06-06T20:45:15.004Z @13:45:14.84 -> showStatus @13:45:16.86 -> Cmd.loadCartridge -10 @13:45:16.94 Gate.power :main,:ON,limit to 5000mA @13:45:16.96 Gate.power :analytic1,:ON,limit to 5000mA @13:45:16.98 Gate.power :analytic2,:ON,limit to 5000mA @13:45:17.01 Gate.power :analytic3,:ON,limit to 5000mA @13:45:17.20 Powering core bus @13:45:19.41 SPR Module 1, FW Rev. B, FPGA Rev. C @13:45:19.91 Archive.configure :default @13:45:20.04 Air.configure :default @13:45:20.14 Lysis1.configure :default @13:45:20.26 Lysis2.configure :default @13:45:20.38 Plunge.configure :default @13:45:20.52 SamplePump.configure :default @13:45:20.65 Toroid.configure :default @13:45:20.77 Carousel.configure :default @13:45:20.90 Pressure.configure :default @13:45:21.03 ExtraPump.configure :default @13:45:21.14 PinPort.configure :default @13:45:21.20 All elf controllers running firmware version 4.79 @13:45:21.22 Gate.power :motor,:ON,limit to 5000mA @13:45:21.33 Archive.in :default @13:45:21.60 Archive.home.jog 0 @13:45:21.86 Toroid.in :default @13:45:21.94 Lysis2.in :default @13:45:21.99 Air.in :default @13:45:22.03 PinPort.in :default @13:45:22.07 Lysis1.in :default @13:45:22.11 Plunge.in :default @13:45:22.33 Toroid.home.jog 0 @13:45:22.38 Lysis2.home.jog 0 @13:45:22.41 Air.home.jog 0 @13:45:22.43 PinPort.home.jog 0 @13:45:22.46 Plunge.home.jog 0 @13:45:22.53 Lysis1.home.jog 0 @13:45:23.20 Archive.in :homing @13:45:23.43 Archive.jog 1388 @13:45:23.92 PinPort.in :homing @13:45:24.00 Plunge.in :homing @13:45:24.10 Lysis2.in :homing @13:45:24.21 Air.in :homing @13:45:24.31 Toroid.in :homing @13:45:24.36 Lysis1.in :homing @13:45:24.54 PinPort.jog -108 @13:45:24.57 Lysis2.jog 1390 @13:45:24.62 Plunge.jog 1554 @13:45:24.65 Air.jog 1387 @13:45:24.69 Toroid.jog 634 @13:45:24.75 Lysis1.jog 1379 @13:45:24.97 PinPort.setRawPosition! 0 @13:45:24.98 Archive.setRawPosition! 50 @13:45:25.05 PinPort.seek :home @13:45:25.09 Archive.seek :home @13:45:25.22 PinPort.in :default @13:45:25.42 Archive.in :default @13:45:25.90 Lysis2.setRawPosition! 50 @13:45:25.96 Lysis2.seek :home @13:45:26.08 Plunge.setRawPosition! 50 @13:45:26.10 Lysis1.setRawPosition! 50 @13:45:26.12 Air.setRawPosition! 50 @13:45:26.21 Plunge.seek :home @13:45:26.28 Air.seek :home @13:45:26.32 Lysis1.seek :home @13:45:26.49 Lysis2.in :default @13:45:26.61 Plunge.in :default @13:45:27.12 Lysis1.in :default @13:45:27.19 Air.in :default @13:45:27.32 Toroid.setRawPosition! 0 @13:45:27.37 Toroid.seek :home @13:45:27.47 Toroid.in :default @13:45:27.61 Toroid.seek :clear @13:45:29.22 Carousel.in :default @13:45:29.29 Carousel.home.jog 0 @13:45:29.77 Carousel.in :default @13:45:29.84 Carousel.seek 20 @13:45:30.00 Selecting Cartridge 19 @13:45:30.26 Carousel.seek 19 @13:45:30.74 Lysis1.in :fast @13:45:30.81 Lysis1.seek :locked @13:45:33.39 Plunge.seek :locked @13:45:33.53 Air.in :fast @13:45:33.59 Air.seek :locked @13:45:36.05 Powering cartridge bus @13:45:38.66 Gate.setIR 1750,5000,0,6 @13:45:38.78 Gate.setIR 0,5000,0,6 @13:45:38.85 PuckHeater.configure :default @13:45:38.90 Gate.power :heat,:ON,limit to 5000mA @13:45:39.57 Mounted lysis Cartridge board#Q05038 @13:45:39.60 Using default puck heat loss curve for HeatingAccelInCarousel @13:45:57.26 -> Cmd.startFiltering 92.07@13:45:57.51 Preparing to filter cartridge 19 @13:45:57.78 Priming sample loop w/100ml, bypass w/10ml @13:45:57.79 SamplePump.setPosition! 0ml @13:45:57.83 Bypass.open @13:45:59.28 Intake.open @13:46:00.03 Sea 14.1m deep (35.6psia) @13:46:00.05 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:46:02.62 Vacuum tare is 54% of full scale @13:46:02.75 Intake.close @13:46:02.81 Bypass.close @13:46:03.55 Verifying toroid holds at least 0.6..0.85 of 35.5psia @13:46:03.56 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @13:46:08.14 Toroid pressure rose 2% to 36.2psia @13:46:08.15 Bypass.open @13:46:08.84 Intake.open @13:46:10.24 Pressure.in :default @13:46:10.30 Pressure.hold @13:46:10.48 SamplePump.in :prime @13:46:10.54 SamplePump.seek 10ml,59 seconds @13:46:19.16 Pressure.stop @13:46:19.21 SamplePump.setPosition! 0ml @13:46:19.26 Exhaust.open @13:46:20.75 Bypass.close @13:46:22.25 Pressure.in :default @13:46:22.31 Pressure.hold @13:46:22.42 Sea 14.6m deep (36.3psia) @13:46:22.56 SamplePump.in :prime @13:46:22.62 SamplePump.seek 100ml,5:16 @13:47:55.60 Pressure.stop @13:47:55.65 Bypass.open @13:47:56.32 Intake.close @13:47:57.09 Toroid.in :hold @13:47:57.15 Toroid.seek :crack @13:47:58.66 Toroid.seek :cartridge @13:47:59.75 Toroid.in :default @13:47:59.81 Toroid.seek :clear @13:48:01.77 Exhaust.close @13:48:01.81 Bypass.close @13:48:02.56 Verifying cartridge holds at least 0.65..0.85 of 36.8psia @13:48:02.58 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @13:48:07.20 Sampling 1000.0ml @13:48:07.22 SamplePump.setPosition! 0ml @13:48:07.28 Exhaust.open @13:48:08.00 Intake.open @13:48:08.84 Pressure.in :default @13:48:08.90 Pressure.hold @13:48:09.02 Sea 14.9m deep (36.7psia) @13:48:09.16 SamplePump.in :sample @13:48:09.22 SamplePump.seek 1000ml,1:23:48 @13:48:11.87 Cartridge 19 filtering 1/1000ml at 0.43ml/s with 23.2psi vacuum 14.1m deep (35.6psia) @13:49:11.86 Cartridge 19 filtering 26/1000ml at 0.42ml/s with 27.8psi vacuum 13.0m deep (33.9psia) @13:50:11.86 Cartridge 19 filtering 51/1000ml at 0.39ml/s with 26.6psi vacuum 13.2m deep (34.2psia) @13:51:11.86 Cartridge 19 filtering 74/1000ml at 0.37ml/s with 28.2psi vacuum 13.4m deep (34.5psia) @13:52:11.87 Cartridge 19 filtering 96/1000ml at 0.37ml/s with 26.4psi vacuum 15.0m deep (36.8psia) @13:53:11.86 Cartridge 19 filtering 118/1000ml at 0.38ml/s with 22.1psi vacuum 15.9m deep (38.1psia) @13:54:11.87 Cartridge 19 filtering 139/1000ml at 0.33ml/s with 23.3psi vacuum 13.8m deep (35.1psia) @13:55:11.86 Cartridge 19 filtering 159/1000ml at 0.31ml/s with 24.7psi vacuum 13.7m deep (35.0psia) @13:56:11.87 Cartridge 19 filtering 179/1000ml at 0.30ml/s with 27.1psi vacuum 13.7m deep (34.9psia) @13:57:11.86 Cartridge 19 filtering 198/1000ml at 0.30ml/s with 23.1psi vacuum 13.9m deep (35.2psia) @13:58:11.87 Cartridge 19 filtering 216/1000ml at 0.32ml/s with 22.7psi vacuum 14.4m deep (36.0psia) @13:59:11.86 Cartridge 19 filtering 234/1000ml at 0.27ml/s with 23.9psi vacuum 13.9m deep (35.2psia) @14:00:11.87 Cartridge 19 filtering 252/1000ml at 0.26ml/s with 24.6psi vacuum 13.0m deep (33.8psia) @14:01:11.86 Cartridge 19 filtering 269/1000ml at 0.28ml/s with 20.5psi vacuum 15.2m deep (37.2psia) @14:02:11.87 Cartridge 19 filtering 285/1000ml at 0.27ml/s with 22.8psi vacuum 13.7m deep (34.9psia) @14:03:11.86 Cartridge 19 filtering 301/1000ml at 0.25ml/s with 24.4psi vacuum 15.3m deep (37.3psia) @14:04:11.92 Cartridge 19 filtering 316/1000ml at 0.24ml/s with 23.1psi vacuum 13.5m deep (34.6psia) @14:05:11.86 Cartridge 19 filtering 331/1000ml at 0.24ml/s with 24.4psi vacuum 13.6m deep (34.8psia) @14:06:11.87 Cartridge 19 filtering 346/1000ml at 0.22ml/s with 24.5psi vacuum 13.8m deep (35.0psia) @14:07:11.86 Cartridge 19 filtering 361/1000ml at 0.24ml/s with 24.5psi vacuum 14.7m deep (36.4psia) @14:08:11.87 Cartridge 19 filtering 375/1000ml at 0.25ml/s with 22.7psi vacuum 14.1m deep (35.6psia) @14:09:11.87 Cartridge 19 filtering 388/1000ml at 0.21ml/s with 24.0psi vacuum 14.2m deep (35.6psia) @14:10:11.87 Cartridge 19 filtering 402/1000ml at 0.22ml/s with 24.7psi vacuum 13.4m deep (34.5psia) @14:11:11.87 Cartridge 19 filtering 415/1000ml at 0.22ml/s with 24.1psi vacuum 12.9m deep (33.8psia) @14:12:11.86 Cartridge 19 filtering 427/1000ml at 0.20ml/s with 24.1psi vacuum 13.8m deep (35.1psia) @14:13:11.86 Cartridge 19 filtering 440/1000ml at 0.21ml/s with 23.3psi vacuum 12.8m deep (33.6psia) @14:14:12.33 Cartridge 19 filtering 452/1000ml at 0.20ml/s with 24.7psi vacuum 13.9m deep (35.2psia) @14:15:11.86 Cartridge 19 filtering 464/1000ml at 0.20ml/s with 23.8psi vacuum 12.7m deep (33.5psia) @14:16:11.92 Cartridge 19 filtering 477/1000ml at 0.20ml/s with 26.0psi vacuum 13.0m deep (33.9psia) @14:16:35.18 Flow averaged 0.28ml/s over 28:27 @14:16:35.22 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @14:16:35.80 Relieved excess vacuum in .6 seconds @14:16:35.82 SamplePump.seek 1000ml,43:42 @14:17:11.86 Cartridge 19 filtering 489/1000ml at 0.20ml/s with 24.2psi vacuum 12.7m deep (33.4psia) @14:18:11.87 Cartridge 19 filtering 501/1000ml at 0.21ml/s with 23.9psi vacuum 13.7m deep (35.0psia) @14:18:29.14 Flow averaged 0.20ml/s over 1:54 @14:18:29.17 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @14:18:29.74 Relieved excess vacuum in .6 seconds @14:18:29.77 SamplePump.seek 1000ml,41:47 @14:18:33.27 Flow estimate reduced to 0.16ml/s @14:18:33.29 Intake.close @14:18:33.35 Exhaust.close @14:18:33.54 Toroid.in :hold @14:18:33.60 Toroid.seek :bypass @14:18:35.22 Toroid.in :default @14:18:35.29 Toroid.seek :clear @14:18:36.90 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @14:18:36.92 Pressure.stop @14:18:37.10 Sampled 505.1ml @14:18:39.23 -> Cmd.startProcessing @14:18:40.68 SPR.command \"read_sequences\" @14:18:40.72 SPR.command \"stats_on\" @14:18:40.82 SPR.command \"start 0 1\" @14:18:41.56 SPR.start /var/log/esp/spr22jun0614h.spr.bz2 @14:18:43.52 Handoff.select :waste @14:18:50.20 Syringe tare is 19% of full scale @14:18:50.22 PinPort.in :locking @14:18:50.29 PinPort.seek :locked @14:18:50.77 Archive.in :fast @14:18:50.83 Lysis2.in :fast @14:18:50.89 Lysis1.seek 1350uL @14:18:51.01 Archive.seek :full @14:18:51.06 Lysis2.seek :full @14:18:53.04 Plunge.seek :reagent @14:18:53.91 Evacuating puck with filter bubble point of 50psi @14:18:53.94 PinPort.seek :locked @14:18:54.03 Handoff.select :waste @14:19:00.61 Air syringe pumping up to 1 stroke to reach 8psi @14:19:00.62 Air stroke #1 of 1 @14:19:00.71 Air.in :pressurize @14:19:00.78 Air.seek :pressurized @14:19:12.39 0.2psi after pressurize @14:19:12.41 Air.in :compress @14:19:12.48 Air.seek :evac @14:19:16.56 0.4psi after compress @14:19:29.13 0.1psi after compress (stable) @14:19:29.15 Air.in :default @14:19:29.22 Air.hold @14:19:29.43 at 0.0psi Opened to waste @14:19:29.44 Handoff.select :blocked @14:19:29.58 Archive syringe pumping up to 8 strokes to reach 50psi @14:19:29.59 Archive stroke #1 of 8 @14:19:29.67 Archive.in :pressurize @14:19:29.74 Archive.seek :pressurized @14:19:42.59 9.8psi after pressurize @14:19:42.63 Archive.in :compress @14:19:42.72 Archive.seek :empty @14:19:57.40 27.2psi after compress @14:20:15.97 37.5psi after compress (stable) @14:20:15.99 Archive.in :gulp @14:20:16.07 Archive.seek :topVent @14:20:23.23 38.2psi after gulp @14:20:35.80 37.9psi after gulp (stable) @14:20:35.81 Archive stroke #2 of 8 @14:20:35.91 Archive.in :pressurize @14:20:35.98 Archive.seek :pressurized @14:20:48.85 37.3psi after pressurize @14:20:48.89 Archive.in :compress @14:20:48.96 Archive.seek :empty @14:21:02.77 Reached 50.3 of 50.0psi after pumping 3442uL (1.99 strokes) @14:21:02.79 Archive.in :default @14:21:02.86 Archive.hold @14:21:02.93 Handoff.select :waste @14:21:13.11 delay 1:00 @14:22:13.13 Handoff.select :blocked @14:22:53.81 Handoff.select :waste @14:22:53.93 6.7psi at evacEnd @14:22:53.94 Handoff.select :blocked @14:22:54.07 Archive.in :fast @14:22:54.13 Archive.seek :topVent @14:23:07.73 Stable @6.8psi after Handoff blocked @14:23:07.75 Archive syringe pumping up to 8 strokes to reach 37psi @14:23:07.76 Archive stroke #1 of 8 @14:23:07.88 Archive.in :pressurize @14:23:07.96 Archive.seek :pressurized @14:23:20.81 8.3psi after pressurize @14:23:20.82 Archive.in :compress @14:23:20.90 Archive.seek :empty @14:23:35.58 11.3psi after compress @14:24:24.65 29.0psi after compress (stable) @14:24:24.67 Archive.in :gulp @14:24:24.74 Archive.seek :topVent @14:24:31.90 30.1psi after gulp @14:24:44.47 30.9psi after gulp (stable) @14:24:44.48 Archive stroke #2 of 8 @14:24:44.57 Archive.in :pressurize @14:24:44.64 Archive.seek :pressurized @14:24:57.49 31.8psi after pressurize @14:24:57.51 Archive.in :compress @14:24:57.61 Archive.seek :empty @14:25:12.29 34.0psi after compress @14:25:16.38 Reached 36.8 of 36.8psi after pumping 3460uL (2.00 strokes) @14:25:16.39 Archive.in :default @14:25:16.47 Archive.hold @14:25:16.56 Handoff.select :waste @14:25:34.24 delay 1:00 @14:26:34.25 Handoff.select :blocked @14:26:56.93 Handoff.select :waste @14:26:57.04 2.2psi at evacEnd @14:26:57.06 Air.seek :fullVent @14:26:58.48 Archive.in :fast @14:26:58.54 Archive.seek :topVent @14:27:05.63 Seawater evacuated, Presure above/below filter released. Lysis begins @14:27:05.72 Lysis1.in :slow @14:27:05.78 Lysis1.seek 1050uL @14:27:35.79 delay 5 seconds @14:27:40.81 Handoff.select :blocked @14:27:40.98 Lysis1.seek 450uL @14:28:40.91 delay 3 seconds @14:28:43.98 Air.in mix with 1.40048 parts Lysis1 @14:28:44.46 Air.seek :evac @14:28:44.52 Lysis1.seek 300uL @14:28:59.63 Lysis1.seek :empty @14:29:29.72 Lysis buffer delivered @15.3psi @14:29:29.74 Polling Can every 2:00 @14:29:30.28 PuckHeater.configure :default #ambient 16.1C @14:29:30.33 PuckHeater.seek 80C @14:30:37.49 PuckHeater.hold 2:00,80C @14:32:37.32 Polling Can every 2:00 @14:32:37.86 PuckHeater.configure :default #ambient 16.1C @14:32:37.91 PuckHeater.hold 30 seconds,79C @14:33:08.03 Polling Can every 2:00 @14:33:08.57 PuckHeater.configure :default #ambient 16.1C @14:33:08.62 PuckHeater.hold 30 seconds,78.5C @14:33:38.75 Polling Can every 2:00 @14:33:39.30 PuckHeater.configure :default #ambient 16.1C @14:33:39.36 PuckHeater.hold 10:00,78C @14:43:38.12 Lysis complete. Syringe pressure @17.5psi @14:43:38.13 Handoff.select :blocked @14:43:38.18 Archive syringe pumping up to 5 strokes to reach 40psi @14:43:38.19 Archive stroke #1 of 5 @14:43:38.28 Archive.in :pressurize @14:43:38.35 Archive.seek :pressurized @14:43:51.21 17.2psi after pressurize @14:43:51.23 Archive.in :compress @14:43:51.31 Archive.seek :empty @14:44:00.03 Reached 40.6 of 40.0psi after pumping 1602uL (0.93 strokes) @14:44:00.05 Archive.in :default @14:44:00.12 Archive.hold @14:44:00.22 delay 2 seconds @14:44:02.24 Archive.in :fast @14:44:02.30 Archive.seek :full @14:44:09.02 Lysis1.seek 138uL @14:44:22.88 delay 1 second @14:44:23.91 Archive.seek :full @14:44:23.95 Lysis1.seek :empty @14:44:37.92 Initial air pressure @50.4psi @14:44:38.02 Lysis1.in :extract @14:44:38.11 Lysis1.seek 600uL,nil @14:44:40.94 Archive syringe pumping up to 6 strokes to reach 25psi @14:44:40.95 Archive stroke #1 of 6 @14:44:41.05 Archive.in :pressurize @14:44:41.13 Archive.seek :pressurized @14:44:54.00 15.8psi after pressurize @14:44:54.02 Archive.in :compress @14:44:54.12 Archive.seek :empty @14:45:08.80 15.9psi after compress @14:45:09.43 Archive.in :default @14:45:09.48 Archive.hold @14:45:09.63 Initial 600uL lysate @20.9psi @14:45:09.64 Handoff.select :blocked @14:45:09.74 Initial air pressure @21.8psi @14:45:09.81 Lysis1.seek 825uL,nil @14:45:11.00 Archive syringe pumping up to 6 strokes to reach 17psi @14:45:11.01 Archive stroke #1 of 6 @14:45:11.12 Archive.in :compress @14:45:11.20 Archive.seek :empty @14:45:11.44 14.5psi after compress @14:45:24.01 15.8psi after compress (stable) @14:45:24.11 Archive.in :default @14:45:24.16 Archive.hold @14:45:24.39 Recovered 825uL lysate @16.0psi @14:45:24.40 Air.in :halfSpeed @14:45:24.48 Air.seek :fullVent @14:45:26.68 Puck Top Vented, bottom @0.9psi @14:45:26.70 Handoff.select :waste @14:45:26.83 delay 5 seconds @14:45:31.91 Handoff open to waste, bottom @-0.0psi @14:45:31.93 Air.in :default @14:45:32.01 Air.seek :evac @14:45:33.43 Lysis2.in :DAmix @14:45:33.55 Lysis1.in :DAmix @14:45:34.07 Lysis2.seek 1480uL @14:45:34.11 Lysis1.seek 575uL @14:46:09.17 delay 5 seconds @14:46:14.19 Handoff.select SPR @14:46:14.31 SPR.command \"start 2 14\" @14:46:14.94 SPR stabilized @14:46:15.08 delay 10 seconds @14:46:25.10 SPR.command \"start 1 0\" @14:46:28.30 Waiting up to 4:00 for SPR to enter state 1 @14:48:30.67 SPR.command \"hold_on\" @14:48:32.09 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @14:48:32.60 Lysis2.seek 1180uL @14:48:32.68 Lysis1.seek 275uL @14:49:14.64 delay 15 seconds @14:49:29.66 SPR.command \"hold_off\" @14:49:33.23 Waiting up to 38:00 for SPR to enter state 7 @15:10:23.51 SPRsummary:0.384,117.384,123.064,152.263 after 3252s @15:17:00.74 SPR analysis completed @15:17:06.69 Ended log spr22jun0614h.spr.bz2 @15:17:06.71 SPR.command \"stats_off\" @15:17:08.03 -> Cmd.stop @15:17:08.13 Handoff.select :close @15:17:08.26 Gate.power :heat,:OFF @15:17:08.27 Gate.power :cartridge,:OFF @15:17:08.36 Carousel.seek 19 @15:17:08.55 Plunge.seek :locked @15:17:09.40 PinPort.in :unlocking @15:17:09.47 PinPort.seek :clear @15:17:10.01 Archive.in :unlocking @15:17:10.14 Archive.seek :clear @15:17:10.23 Air.in :unlocking @15:17:10.35 Lysis1.in :unlocking @15:17:10.39 Air.seek :clear @15:17:10.55 Lysis1.seek :clear @15:17:10.59 Lysis2.in :unlocking @15:17:10.70 Lysis2.seek :clear @15:17:14.29 Gate.power :motor,:OFF @15:17:14.31 Gate.power :core,:OFF @15:17:14.34 Gate.power :analytic1,:OFF @15:17:14.36 Gate.power :analytic2,:OFF @15:17:14.39 Gate.power :analytic3,:OFF @15:17:14.41 Gate.power :main,:OFF @15:17:14.43 Safely stopped and ready to power off ##-- file closed 2022-06-07T08:16:07.150Z