##-- file opened 2019-08-27T09:53:59.974Z @05:54:00.19 -> showStatus @05:54:01.80 -> Cmd.loadCartridge -4 @05:54:01.94 Gate.power :main,:ON,limit to 5000mA @05:54:01.95 Gate.power :analytic1,:ON,limit to 5000mA @05:54:01.98 Gate.power :analytic2,:ON,limit to 5000mA @05:54:02.00 Gate.power :analytic3,:ON,limit to 5000mA @05:54:02.13 Powering core bus @05:54:04.42 SPR Module 1, FW Rev. B, FPGA Rev. C @05:54:04.55 Archive.configure :default @05:54:04.67 Air.configure :default @05:54:04.77 Lysis1.configure :default @05:54:04.87 Lysis2.configure :default @05:54:04.99 Plunge.configure :default @05:54:05.11 SamplePump.configure :default @05:54:05.23 ExtraPump.configure :default @05:54:05.36 Toroid.configure :default @05:54:05.48 Carousel.configure :default @05:54:05.60 Pressure.configure :default @05:54:05.71 PinPort.configure :default @05:54:05.77 All elf controllers running firmware version 4.58 @05:54:05.80 Gate.power :motor,:ON,limit to 5000mA @05:54:06.03 Archive.reconfigure :default @05:54:06.11 Air.reconfigure :default @05:54:06.36 Archive.home.jog 0 @05:54:06.41 Air.home.jog 0 @05:54:06.55 Toroid.reconfigure :default @05:54:06.63 Plunge.reconfigure :default @05:54:06.67 Lysis1.reconfigure :default @05:54:06.75 PinPort.reconfigure :default @05:54:06.79 Lysis2.reconfigure :default @05:54:07.00 Lysis2.home.jog 0 @05:54:07.05 Plunge.home.jog 0 @05:54:07.10 Lysis1.home.jog 0 @05:54:07.16 PinPort.home.jog 0 @05:54:07.22 Toroid.home.jog 0 @05:54:07.41 Archive.reconfigure :homing @05:54:07.48 Air.reconfigure :homing @05:54:07.72 Archive.jog 1379 @05:54:07.78 Air.jog 1406 @05:54:07.93 Toroid.reconfigure :homing @05:54:07.96 Plunge.reconfigure :homing @05:54:08.00 PinPort.reconfigure :homing @05:54:08.05 Lysis1.reconfigure :homing @05:54:08.09 Lysis2.reconfigure :homing @05:54:08.29 Lysis1.jog 1400 @05:54:08.34 Lysis2.jog 1427 @05:54:08.40 Toroid.jog 629 @05:54:08.45 PinPort.jog -107 @05:54:08.51 Plunge.jog 389 @05:54:08.68 PinPort.setRawPosition! 0 @05:54:08.72 PinPort.seek :home @05:54:08.82 PinPort.reconfigure :default @05:54:08.90 Archive.setRawPosition! 50 @05:54:08.95 Archive.seek :home @05:54:09.03 Air.setRawPosition! 50 @05:54:09.08 Air.seek :home @05:54:09.17 Plunge.setRawPosition! 50 @05:54:09.22 Plunge.seek :home @05:54:09.73 Plunge.reconfigure :default @05:54:09.77 Lysis1.setRawPosition! 50 @05:54:09.80 Air.reconfigure :default @05:54:09.82 Lysis2.setRawPosition! 50 @05:54:09.85 Archive.reconfigure :default @05:54:10.00 Lysis1.seek :home @05:54:10.06 Lysis2.seek :home @05:54:10.37 Lysis1.reconfigure :default @05:54:10.46 Lysis2.reconfigure :default @05:54:10.54 Toroid.setRawPosition! 0 @05:54:10.59 Toroid.seek :home @05:54:10.69 Toroid.reconfigure :default @05:54:10.84 Toroid.seek :clear @05:54:12.47 Carousel.reconfigure :default @05:54:12.54 Carousel.home.jog 0 @05:54:13.04 Carousel.seek 8 @05:54:13.21 Selecting Cartridge 9 @05:54:13.45 Carousel.seek 9 @05:54:13.94 Lysis1.seek :locked @05:54:18.06 Plunge.seek :locked @05:54:18.18 Air.seek :locked @05:54:22.31 Powering cartridge bus @05:54:24.43 Gate.setIR 1750,5000,0,6 @05:54:24.56 Gate.setIR 0,5000,0,6 @05:54:24.60 Loaded Archive Cartridge @05:54:24.61 Gate.power :cartridge,:OFF @05:54:40.20 -> Cmd.startFiltering @05:54:40.42 Duration of filtering limited to 2:05:00 @05:57:23.68 Pressure.reconfigure :default @05:57:23.74 Pressure.hold @05:57:23.96 SamplePump.reconfigure :sample @05:57:24.42 SamplePump.seek 1000ml,1:23:53 @05:57:26.72 Cartridge 9 sampling 1/1000ml at 0.35ml/s with 24.4psi vacuum @05:58:26.72 Cartridge 9 sampling 20/1000ml at 0.22ml/s with 22.7psi vacuum @05:58:53.56 Flow averaged 0.31ml/s over 1:23 @05:58:53.59 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @05:58:54.59 Relieved excess vacuum in 7.8 seconds @05:58:54.61 SamplePump.seek 1000ml,1:21:46 @05:58:56.52 Flow estimate reduced to 0.25ml/s @05:58:56.55 Waiting up to 14 seconds for vacuum to reduce from 28.2 to 18.0psi @05:58:57.55 Relieved excess vacuum in 2.8 seconds @05:58:57.57 SamplePump.seek 1000ml,1:21:44 @05:58:59.43 Flow estimate reduced to 0.20ml/s @05:58:59.46 Intake.close @05:58:59.52 Exhaust.close @05:58:59.68 Toroid.reconfigure :hold @05:58:59.75 Toroid.seek :bypass @05:59:01.37 Toroid.reconfigure :default @05:59:01.44 Toroid.seek :clear @05:59:03.06 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @05:59:03.08 Pressure.coast @05:59:03.26 Sampled 26.1ml @05:59:05.53 -> Cmd.startProcessing @05:59:05.78 Plunge.seek :reagent @05:59:06.64 Sample loop closed. Ready to Deliver Reagents @05:59:06.65 Archive.seek :sealed @05:59:10.92 Archive.seek :evac @05:59:24.86 delay 1:00 @06:00:24.89 Archive.seek :clear @06:00:42.60 RNAlater soak for 10:00 @06:10:42.63 Archive.seek :tVent @06:10:45.21 delay 1 second @06:10:46.23 Archive.seek :evac @06:11:01.85 delay 10 seconds @06:11:11.88 Archive.seek :tVent @06:11:27.50 delay 1 second @06:11:28.52 Archive.seek :evac @06:11:44.14 delay 10 seconds @06:11:54.17 Sample preserved and evacuated @06:11:55.39 -> Cmd.stop @06:11:55.53 Carousel.seek 9 @06:11:55.70 Plunge.seek :locked @06:11:56.54 PinPort.seek :clear @06:11:56.68 Archive.seek :clear @06:11:56.81 Air.seek :clear @06:11:56.94 Lysis1.seek :clear @06:12:14.42 Archive.coast @06:12:14.47 Air.coast @06:12:14.53 Lysis1.coast @06:12:14.58 Lysis2.coast @06:12:14.63 Plunge.coast @06:12:14.67 SamplePump.coast @06:12:14.72 Toroid.coast @06:12:14.77 Carousel.coast @06:12:14.83 PinPort.coast @06:12:15.01 Gate.power :motor,:OFF @06:12:15.02 Gate.power :core,:OFF @06:12:15.05 Gate.power :analytic1,:OFF @06:12:15.07 Gate.power :analytic2,:OFF @06:12:15.08 Gate.power :analytic3,:OFF @06:12:15.11 Gate.power :main,:OFF @06:12:15.13 Safely stopped and ready to power off ##-- file closed 2019-08-27T10:28:04.406Z ##-- file opened 2019-08-27T10:28:04.420Z @06:28:04.45 -> showStatus @06:28:06.47 -> Cmd.loadCartridge -15 @06:28:06.61 Gate.power :main,:ON,limit to 5000mA @06:28:06.62 Gate.power :analytic1,:ON,limit to 5000mA @06:28:06.66 Gate.power :analytic2,:ON,limit to 5000mA @06:28:06.68 Gate.power :analytic3,:ON,limit to 5000mA @06:28:06.82 Powering core bus @06:28:09.09 SPR Module 1, FW Rev. B, FPGA Rev. C @06:28:09.23 Archive.configure :default @06:28:09.35 Air.configure :default @06:28:09.45 Lysis1.configure :default @06:28:09.55 Lysis2.configure :default @06:28:09.67 Plunge.configure :default @06:28:09.79 SamplePump.configure :default @06:28:09.92 ExtraPump.configure :default @06:28:10.06 Toroid.configure :default @06:28:10.18 Carousel.configure :default @06:28:10.30 Pressure.configure :default @06:28:10.40 PinPort.configure :default @06:28:10.46 All elf controllers running firmware version 4.58 @06:28:10.49 Gate.power :motor,:ON,limit to 5000mA @06:28:10.72 Archive.reconfigure :default @06:28:10.78 Air.reconfigure :default @06:28:11.06 Archive.home.jog 0 @06:28:11.12 Air.home.jog 0 @06:28:11.25 Toroid.reconfigure :default @06:28:11.33 Plunge.reconfigure :default @06:28:11.37 Lysis1.reconfigure :default @06:28:11.45 Lysis2.reconfigure :default @06:28:11.54 PinPort.reconfigure :default @06:28:11.74 PinPort.home.jog 0 @06:28:11.79 Plunge.home.jog 0 @06:28:11.85 Lysis2.home.jog 0 @06:28:11.91 Toroid.home.jog 0 @06:28:11.97 Lysis1.home.jog 0 @06:28:12.14 Archive.reconfigure :homing @06:28:12.22 Air.reconfigure :homing @06:28:12.46 Air.jog 1403 @06:28:12.51 Archive.jog 1381 @06:28:12.67 Toroid.reconfigure :homing @06:28:12.70 Plunge.reconfigure :homing @06:28:12.74 PinPort.reconfigure :homing @06:28:12.79 Lysis1.reconfigure :homing @06:28:12.85 Lysis2.reconfigure :homing @06:28:13.02 Lysis1.jog 1399 @06:28:13.08 Lysis2.jog 1427 @06:28:13.14 Toroid.jog 630 @06:28:13.20 PinPort.jog -107 @06:28:13.26 Plunge.jog 1534 @06:28:13.43 PinPort.setRawPosition! 0 @06:28:13.47 PinPort.seek :home @06:28:13.56 PinPort.reconfigure :default @06:28:13.66 Air.setRawPosition! 50 @06:28:13.70 Air.seek :home @06:28:13.78 Archive.setRawPosition! 50 @06:28:13.83 Archive.seek :home @06:28:13.98 Air.reconfigure :default @06:28:14.50 Plunge.setRawPosition! 50 @06:28:14.53 Lysis2.setRawPosition! 50 @06:28:14.55 Lysis1.setRawPosition! 50 @06:28:14.57 Archive.reconfigure :default @06:28:14.73 Plunge.seek :home @06:28:14.77 Lysis1.seek :home @06:28:14.82 Lysis2.seek :home @06:28:15.11 Plunge.reconfigure :default @06:28:15.21 Lysis1.reconfigure :default @06:28:15.23 Lysis2.reconfigure :default @06:28:15.36 Toroid.setRawPosition! 0 @06:28:15.41 Toroid.seek :home @06:28:15.50 Toroid.reconfigure :default @06:28:15.68 Toroid.seek :clear @06:28:17.31 Carousel.reconfigure :default @06:28:17.38 Carousel.home.jog 0 @06:28:17.87 Carousel.seek 9 @06:28:18.01 Selecting Cartridge 6 @06:28:18.26 Carousel.seek 6 @06:28:19.15 Lysis1.seek :locked @06:28:23.28 Plunge.seek :locked @06:28:23.40 Air.seek :locked @06:28:27.53 Powering cartridge bus @06:28:29.65 Gate.setIR 1750,5000,0,6 @06:28:29.78 Gate.setIR 0,5000,0,6 @06:28:29.83 Loaded Lyse-n-Go Cartridge @06:28:29.84 PuckHeater.configure HeatConfig @06:28:29.90 Gate.power :heat,:ON,limit to 5000mA @06:28:45.40 -> Cmd.startFiltering @06:28:45.63 Duration of filtering limited to 2:05:00 @06:28:45.89 Priming sample loop w/100ml, bypass w/10ml @06:28:45.91 SamplePump.setPosition! 0ml @06:28:45.95 Bypass.open @06:28:47.43 Intake.open @06:28:48.22 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:28:51.06 Vacuum tare is 52% of full scale @06:28:51.19 Pressure.reconfigure :default @06:28:51.25 Pressure.hold @06:28:51.42 SamplePump.reconfigure :prime @06:28:51.49 SamplePump.seek 10ml,1:21 @06:29:00.45 Pressure.coast @06:29:00.51 SamplePump.setPosition! 0ml @06:29:00.56 Exhaust.open @06:29:01.95 Bypass.close @06:29:03.48 Pressure.reconfigure :default @06:29:03.54 Pressure.hold @06:29:03.77 SamplePump.reconfigure :prime @06:29:03.84 SamplePump.seek 100ml,8:51 @06:31:01.91 Pressure.coast @06:31:01.98 Bypass.open @06:31:02.71 Intake.close @06:31:03.51 Toroid.reconfigure :hold @06:31:03.58 Toroid.seek :crack @06:31:04.37 Toroid.reconfigure :default @06:31:04.43 Toroid.seek :clear @06:31:05.99 Toroid.reconfigure :hold @06:31:06.06 Toroid.seek :cartridge @06:31:07.87 Toroid.reconfigure :default @06:31:07.93 Toroid.seek :clear @06:31:09.91 Pressurizing cartridge to 26psi above 12.0psia @06:31:09.92 SamplePump.setPosition! 0ml @06:31:09.96 Intake.open @06:31:10.67 Bypass.close @06:31:11.40 Exhaust.close @06:31:12.22 Pressure.reconfigure :default @06:31:12.28 Pressure.hold @06:31:12.44 SamplePump.reconfigure :charge @06:31:12.52 SamplePump.seek 2.3ml,42 seconds @06:31:24.39 Pressure.coast @06:31:24.45 Intake.close @06:31:25.26 Pressurized cartridge with 0.662ml to 26.1psi @06:31:25.27 Isolated @34.5psia (22.5psi) @06:31:25.28 Checking for cartridge leaks from 34.5psia @06:31:25.31 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @06:31:30.41 Sampling 1000.0ml @06:31:30.42 SamplePump.setPosition! 0ml @06:31:30.47 Exhaust.open @06:31:31.20 Intake.open @06:31:32.06 Pressure.reconfigure :default @06:31:32.12 Pressure.hold @06:31:32.73 SamplePump.reconfigure :sample @06:31:32.80 SamplePump.seek 1000ml,1:23:53 @06:31:35.10 Cartridge 6 sampling 1/1000ml at 0.63ml/s with 25.3psi vacuum @06:32:35.10 Cartridge 6 sampling 30/1000ml at 0.42ml/s with 25.0psi vacuum @06:33:35.10 Cartridge 6 sampling 51/1000ml at 0.33ml/s with 23.4psi vacuum @06:34:35.10 Cartridge 6 sampling 68/1000ml at 0.23ml/s with 24.2psi vacuum @06:35:31.44 Flow averaged 0.34ml/s over 3:53 @06:35:31.47 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi @06:35:32.48 Relieved excess vacuum in 7.2 seconds @06:35:32.50 SamplePump.seek 1000ml,1:17:16 @06:35:34.27 Flow estimate reduced to 0.27ml/s @06:35:34.31 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @06:35:35.11 Cartridge 6 sampling 80/1000ml at 0.00ml/s with 6.3psi vacuum @06:35:35.31 Relieved excess vacuum in 2.7 seconds @06:35:35.33 SamplePump.seek 1000ml,1:17:14 @06:35:40.20 Flow estimate reduced to 0.22ml/s @06:35:40.24 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi @06:35:41.24 Relieved excess vacuum in 2.8 seconds @06:35:41.26 SamplePump.seek 1000ml,1:17:09 @06:35:43.03 Flow estimate reduced to 0.17ml/s @06:35:43.04 Intake.close @06:35:43.11 Exhaust.close @06:35:43.27 Toroid.reconfigure :hold @06:35:43.35 Toroid.seek :bypass @06:35:44.97 Toroid.reconfigure :default @06:35:45.04 Toroid.seek :clear @06:35:46.66 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @06:35:46.68 Pressure.coast @06:35:46.87 Sampled 81.0ml @06:35:48.79 -> Cmd.startProcessing @06:35:50.65 SPR.command \"read_sequences\" @06:35:50.68 SPR.command \"stats_on\" @06:35:50.72 SPR.command \"start 0 1\" @06:35:52.73 Begun log /var/log/esp/spr19aug2706h.spr @06:35:53.75 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @06:35:55.30 Handoff.select :waste @06:35:55.47 Archive.seek :empty @06:36:02.64 Syringe tare is 21% of full scale @06:36:13.19 PinPort.seek :cartridge @06:36:17.75 Lysis1.seek 1350ul @06:36:20.23 Lysis2.seek 1700ul @06:36:23.33 Plunge.seek :reagent @06:36:24.18 Evacuating puck with filter bubble point of 50psi @06:36:24.20 PinPort.seek :cartridge @06:36:24.28 Handoff.select :waste @06:36:31.65 Air pumping 1 strokes to reach 8psi @06:36:31.67 Air.reconfigure :gulp @06:36:31.77 Air.seek :tVent @06:36:33.56 0.1psi after gulp @06:36:33.57 Air stroke #1 of 1 @06:36:33.60 Air.reconfigure :pressurize @06:36:33.69 Air.seek :pressurized @06:36:46.53 0.0psi after pressurize @06:36:46.55 Air.reconfigure :compress @06:36:46.63 Air.seek :evac @06:36:49.29 0.3psi after compress @06:37:03.60 0.1psi after compress (stable) @06:37:03.73 at 0.1psi Opened to waste @06:37:03.74 Handoff.select :blocked @06:37:03.94 Air pumping 10 strokes to reach 50psi @06:37:03.95 Air.reconfigure :gulp @06:37:04.04 Air.seek :tVent @06:37:13.93 6.0psi after gulp @06:37:13.94 Air stroke #1 of 10 @06:37:13.97 Air.reconfigure :pressurize @06:37:14.04 Air.seek :pressurized @06:37:26.87 9.4psi after pressurize @06:37:26.89 Air.reconfigure :compress @06:37:26.97 Air.seek :evac @06:37:29.63 11.0psi after compress @06:38:13.51 35.2psi after compress (stable) @06:38:13.52 Air.reconfigure :gulp @06:38:13.60 Air.seek :tVent @06:38:23.48 37.2psi after gulp @06:38:23.49 Air stroke #2 of 10 @06:38:23.51 Air.reconfigure :pressurize @06:38:23.59 Air.seek :pressurized @06:38:36.43 35.2psi after pressurize @06:38:36.44 Air.reconfigure :compress @06:38:36.53 Air.seek :evac @06:38:39.21 33.9psi after compress @06:39:05.36 Reached 50.0 of 50.0psi after pumping 4672ul (3.00 strokes) @06:39:05.37 Handoff.select :waste @06:39:30.38 delay 30 seconds @06:40:00.42 Handoff.select :blocked @06:40:30.96 Handoff.select :waste @06:40:31.09 PSI at @3.39psi at evacEnd @06:40:31.10 Handoff.select :blocked @06:40:31.22 Air.reconfigure :gulp @06:40:31.33 Air.seek :tVent @06:40:48.82 Stable @2.3psi after Handoff blocked @06:40:48.91 Air pumping 10 strokes to reach 32psi @06:40:48.92 Air stroke #1 of 10 @06:40:48.94 Air.reconfigure :pressurize @06:40:49.02 Air.seek :pressurized @06:41:01.86 2.5psi after pressurize @06:41:01.88 Air.reconfigure :compress @06:41:01.96 Air.seek :evac @06:41:04.62 2.9psi after compress @06:41:27.42 15.3psi after compress (stable) @06:41:27.44 Air.reconfigure :gulp @06:41:27.52 Air.seek :tVent @06:41:37.41 16.0psi after gulp @06:41:37.42 Air stroke #2 of 10 @06:41:37.44 Air.reconfigure :pressurize @06:41:37.51 Air.seek :pressurized @06:41:50.35 15.8psi after pressurize @06:41:50.37 Air.reconfigure :compress @06:41:50.44 Air.seek :evac @06:41:53.12 19.2psi after compress @06:42:22.29 27.1psi after compress (stable) @06:42:22.31 Air.reconfigure :gulp @06:42:22.38 Air.seek :tVent @06:42:32.27 27.6psi after gulp @06:42:32.28 Air stroke #3 of 10 @06:42:32.32 Air.reconfigure :pressurize @06:42:32.45 Air.seek :pressurized @06:42:45.28 27.4psi after pressurize @06:42:45.30 Air.reconfigure :compress @06:42:45.39 Air.seek :evac @06:42:48.06 28.9psi after compress @06:42:49.33 Reached 32.5 of 32.3psi after pumping 6230ul (4.00 strokes) @06:42:49.34 Handoff.select :waste @06:43:02.23 delay 30 seconds @06:43:32.26 Handoff.select :blocked @06:43:57.27 Handoff.select :waste @06:43:57.40 PSI at @1.27psi at evacEnd @06:43:57.41 Air.reconfigure :default @06:43:57.53 Air.seek :fVent @06:43:58.97 delay 5 seconds @06:44:03.99 Seawater evacuated, Presure above/below filter released. Lysis begins @06:44:04.06 Lysis1.reconfigure :slow @06:44:04.13 Lysis1.seek 1050ul @06:44:34.16 delay 5 seconds @06:44:39.18 Handoff.select :blocked @06:44:39.34 Lysis1.seek 450ul @06:45:39.31 delay 3 seconds @06:45:42.39 Air.reconfigure to mix into 1.37806 parts Lysis1 @06:45:42.92 Air.seek :evac @06:45:42.96 Lysis1.seek 300ul @06:45:58.07 Air.reconfigure :default @06:45:58.14 Air.seek :full @06:46:12.35 Lysis1.seek :empty @06:46:42.45 Lysis Delivered. Syringe pressure @33.5psi @06:46:42.50 PuckHeater.seek 84.5C @06:47:37.44 PuckHeater.hold 15:00,84.5C @07:02:33.23 Lysis complete. Syringe pressure @37.3psi @07:02:33.24 Handoff.select :blocked @07:02:33.37 Lysis1.reconfigure :halfSpeed @07:02:33.44 Lysis1.seek 200ul @07:02:37.21 delay 1 second @07:02:38.23 Lysis1.seek :empty @07:02:42.07 Initial AirS pressure @37.6psi @07:02:42.13 Lysis1.reconfigure :extract @07:02:42.22 Lysis1.seek 900ul,10:00 @07:02:43.39 Air pumping 10 strokes to reach 21psi @07:02:43.41 Air stroke #1 of 10 @07:02:43.43 Air.reconfigure :pressurize @07:02:43.51 Air.seek :pressurized @07:02:56.35 16.0psi after pressurize @07:02:56.36 Air.reconfigure :compress @07:02:56.44 Air.seek :evac @07:02:59.11 16.5psi after compress @07:03:13.35 15.9psi after compress (stable) @07:03:13.37 Air.reconfigure :gulp @07:03:13.45 Air.seek :tVent @07:03:23.34 15.9psi after gulp @07:03:23.35 Air stroke #2 of 10 @07:03:23.37 Air.reconfigure :pressurize @07:03:23.46 Air.seek :pressurized @07:03:36.30 15.9psi after pressurize @07:03:36.31 Air.reconfigure :compress @07:03:36.39 Air.seek :evac @07:03:39.06 15.9psi after compress @07:03:52.78 Air.reconfigure :default @07:03:52.83 Air.hold @07:03:53.01 Lysis1 at 900ul @14.3psi @07:03:53.03 Lysis1.reconfigure :default @07:03:53.10 Lysis1.seek 925ul @07:03:53.88 Lysis1 at 925ul, bottom @13.7psi after recovery @07:03:54.99 Lysis1 at 925ul, bottom @15.5psi @07:03:55.01 Air.reconfigure :halfSpeed @07:03:55.08 Air.seek :fVent @07:03:57.33 Puck Top Vented, bottom @4.3psi @07:03:57.34 Handoff.select :waste @07:03:57.47 delay 5 seconds @07:04:02.49 delay 5 seconds @07:04:07.51 Handoff.select SPR @07:04:07.62 SPR.command \"start 2 14\" @07:04:08.44 delay 10 seconds @07:04:18.46 SPR.command \"start 2 0\" @07:04:21.69 Waiting up to 11:00 for SPR to enter state 1 @07:12:53.76 SPR.command \"hold_on\" @07:12:55.66 SPR Dilution 1 of 1 -- Running Experiment 2 @07:12:55.67 Lysis2.reconfigure :DAmix @07:12:55.78 Lysis1.reconfigure :DAmix @07:12:56.15 Lysis2.seek 1450ul @07:12:56.19 Lysis1.seek 675ul @07:13:31.62 Lysis2.seek 1150ul @07:13:31.65 Lysis1.seek 375ul @07:14:13.67 volL1 = 300.0 and volL2 = 300.0 @07:14:13.69 delay 15 seconds @07:14:28.71 SPR.command \"hold_off\" @07:14:31.75 Waiting up to 25:00 for SPR to enter state 7 @07:26:07.32 SPR Analysis has Completed @07:26:07.33 Handoff.select :waste @07:26:08.24 Ended log /var/log/esp/spr19aug2706h.spr @07:26:08.25 SPR.command \"stats_off\" @07:26:09.64 -> Cmd.stop @07:26:09.73 Handoff.select :close @07:26:09.86 Gate.power :heat,:OFF @07:26:09.88 Gate.power :cartridge,:OFF @07:26:09.96 Carousel.seek 6 @07:26:10.14 Plunge.seek :locked @07:26:10.98 PinPort.seek :clear @07:26:15.60 Archive.seek :clear @07:26:15.73 Air.reconfigure :default @07:26:15.87 Lysis1.reconfigure :default @07:26:15.98 Air.seek :clear @07:26:16.05 Lysis1.seek :clear @07:26:16.14 Lysis2.reconfigure :default @07:26:16.21 Lysis2.seek :clear @07:26:33.35 Archive.coast @07:26:33.41 Air.coast @07:26:33.45 Lysis1.coast @07:26:33.50 Lysis2.coast @07:26:33.55 Plunge.coast @07:26:33.60 SamplePump.coast @07:26:33.65 Toroid.coast @07:26:33.70 Carousel.coast @07:26:33.75 PinPort.coast @07:26:33.93 Gate.power :motor,:OFF @07:26:33.95 Gate.power :core,:OFF @07:26:33.97 Gate.power :analytic1,:OFF @07:26:33.99 Gate.power :analytic2,:OFF @07:26:34.01 Gate.power :analytic3,:OFF @07:26:34.03 Gate.power :main,:OFF @07:26:34.05 Safely stopped and ready to power off ##-- file closed 2019-08-27T14:58:45.014Z ##-- file opened 2019-08-27T14:58:45.016Z @10:58:44.93 -> showStatus @10:58:46.93 -> Cmd.loadCartridge -4 @10:58:47.07 Gate.power :main,:ON,limit to 5000mA @10:58:47.08 Gate.power :analytic1,:ON,limit to 5000mA @10:58:47.10 Gate.power :analytic2,:ON,limit to 5000mA @10:58:47.14 Gate.power :analytic3,:ON,limit to 5000mA @10:58:47.27 Powering core bus @10:58:49.56 SPR Module 1, FW Rev. B, FPGA Rev. C @10:58:49.68 Archive.configure :default @10:58:49.80 Air.configure :default @10:58:49.90 Lysis1.configure :default @10:58:50.00 Lysis2.configure :default @10:58:50.11 Plunge.configure :default @10:58:50.23 SamplePump.configure :default @10:58:50.35 ExtraPump.configure :default @10:58:50.47 Toroid.configure :default @10:58:50.59 Carousel.configure :default @10:58:50.71 Pressure.configure :default @10:58:50.81 PinPort.configure :default @10:58:50.87 All elf controllers running firmware version 4.58 @10:58:50.89 Gate.power :motor,:ON,limit to 5000mA @10:58:51.01 Archive.reconfigure :default @10:58:51.34 Archive.home.jog 0 @10:58:51.47 Toroid.reconfigure :default @10:58:51.55 Plunge.reconfigure :default @10:58:51.58 Lysis1.reconfigure :default @10:58:51.62 Air.reconfigure :default @10:58:51.65 PinPort.reconfigure :default @10:58:51.73 Lysis2.reconfigure :default @10:58:51.93 Lysis1.home.jog 0 @10:58:51.98 Air.home.jog 0 @10:58:52.04 Toroid.home.jog 0 @10:58:52.10 PinPort.home.jog 0 @10:58:52.16 Plunge.home.jog 0 @10:58:52.21 Lysis2.home.jog 0 @10:58:52.49 Archive.reconfigure :homing @10:58:52.67 Toroid.reconfigure :homing @10:58:52.71 Plunge.reconfigure :homing @10:58:52.74 Air.reconfigure :homing @10:58:52.78 PinPort.reconfigure :homing @10:58:52.83 Lysis1.reconfigure :homing @10:58:53.05 Lysis1.jog 1401 @10:58:53.10 Archive.jog 1380 @10:58:53.15 Air.jog 1410 @10:58:53.21 PinPort.jog -106 @10:58:53.26 Toroid.jog 629 @10:58:53.33 Plunge.jog 1533 @10:58:53.42 Lysis2.reconfigure :homing @10:58:53.48 Lysis2.jog 1418 @10:58:53.55 PinPort.setRawPosition! 0 @10:58:53.60 PinPort.seek :home @10:58:53.68 PinPort.reconfigure :default @10:58:54.20 Lysis1.setRawPosition! 50 @10:58:54.25 Lysis1.seek :home @10:58:54.35 Air.setRawPosition! 50 @10:58:54.37 Archive.setRawPosition! 50 @10:58:54.45 Air.seek :home @10:58:54.48 Archive.seek :home @10:58:55.00 Plunge.setRawPosition! 50 @10:58:55.03 Lysis1.reconfigure :default @10:58:55.06 Lysis2.setRawPosition! 50 @10:58:55.14 Plunge.seek :home @10:58:55.24 Lysis2.seek :home @10:58:55.37 Archive.reconfigure :default @10:58:55.40 Air.reconfigure :default @10:58:55.57 Plunge.reconfigure :default @10:58:55.59 Toroid.setRawPosition! 0 @10:58:55.66 Toroid.seek :home @10:58:55.78 Toroid.reconfigure :default @10:58:55.81 Lysis2.reconfigure :default @10:58:56.22 Toroid.seek :clear @10:58:57.84 Carousel.reconfigure :default @10:58:57.91 Carousel.home.jog 0 @10:58:58.40 Carousel.seek 6 @10:58:58.53 Selecting Cartridge 7 @10:58:58.78 Carousel.seek 7 @10:58:59.26 Lysis1.seek :locked @10:59:03.41 Plunge.seek :locked @10:59:03.54 Air.seek :locked @10:59:07.66 Powering cartridge bus @10:59:09.78 Gate.setIR 1750,5000,0,6 @10:59:09.91 Gate.setIR 0,5000,0,6 @10:59:09.95 Loaded Archive Cartridge Gate.power :cartridge,:OFF @10:59:25.88 -> Cmd.startFiltering @10:59:26.11 Duration of filtering limited to 2:05:00 @10:59:26.38 Priming sample loop w/100ml, bypass w/10ml @10:59:26.39 SamplePump.setPosition! 0ml @10:59:26.43 Bypass.open @10:59:27.91 Intake.open @10:59:28.72 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:59:31.56 Vacuum tare is 52% of full scale @10:59:31.69 Pressure.reconfigure :default @10:59:31.75 Pressure.hold @10:59:31.91 SamplePump.reconfigure :prime @10:59:31.98 SamplePump.seek 10ml,1:21 @10:59:40.95 Pressure.coast @10:59:41.00 SamplePump.setPosition! 0ml @10:59:41.05 Exhaust.open @10:59:42.47 Bypass.close @10:59:44.01 Pressure.reconfigure :default @10:59:44.07 Pressure.hold @10:59:44.29 SamplePump.reconfigure :prime @10:59:44.36 SamplePump.seek 100ml,8:51 @11:01:41.96 Pressure.coast @11:01:42.02 Bypass.open @11:01:42.76 Intake.close @11:01:43.63 Toroid.reconfigure :hold @11:01:43.69 Toroid.seek :crack @11:01:44.49 Toroid.reconfigure :default @11:01:44.55 Toroid.seek :clear @11:01:46.11 Toroid.reconfigure :hold @11:01:46.18 Toroid.seek :cartridge @11:01:47.99 Toroid.reconfigure :default @11:01:48.05 Toroid.seek :clear @11:01:50.01 Pressurizing cartridge to 26psi above 13.4psia @11:01:50.03 SamplePump.setPosition! 0ml @11:01:50.07 Intake.open @11:01:50.85 Bypass.close @11:01:51.57 Exhaust.close @11:01:52.41 Pressure.reconfigure :default @11:01:52.46 Pressure.hold @11:01:52.63 SamplePump.reconfigure :charge @11:01:52.70 SamplePump.seek 2.3ml,42 seconds @11:02:02.84 Pressure.coast @11:02:02.90 Intake.close @11:02:03.72 Pressurized cartridge with 0.481ml to 26.0psi @11:02:03.73 Isolated @34.8psia (21.4psi) @11:02:03.74 Checking for cartridge leaks from 34.8psia @11:02:03.77 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @11:02:08.82 Cartridge pressure fell 2% to 34.3psia @11:02:08.90 Sampling 1000.0ml @11:02:08.91 SamplePump.setPosition! 0ml @11:02:08.96 Exhaust.open @11:02:09.69 Intake.open @11:02:10.55 Pressure.reconfigure :default @11:02:10.61 Pressure.hold @11:02:11.22 SamplePump.reconfigure :sample @11:02:11.29 SamplePump.seek 1000ml,1:23:53 @11:02:13.59 Cartridge 7 sampling 1/1000ml at 0.30ml/s with 25.1psi vacuum @11:03:08.67 Flow averaged 0.27ml/s over 54 seconds @11:03:08.70 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @11:03:09.70 Relieved excess vacuum in 5.2 seconds @11:03:09.72 SamplePump.seek 1000ml,1:22:41 @11:03:11.76 Flow estimate reduced to 0.22ml/s @11:03:11.79 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi @11:03:12.79 Relieved excess vacuum in 2.9 seconds @11:03:12.81 SamplePump.seek 1000ml,1:22:39 @11:03:13.58 Cartridge 7 sampling 15/1000ml at 0.30ml/s with 16.2psi vacuum @11:03:14.84 Flow estimate reduced to 0.17ml/s @11:03:14.86 Intake.close @11:03:14.91 Exhaust.close @11:03:15.07 Toroid.reconfigure :hold @11:03:15.15 Toroid.seek :bypass @11:03:16.77 Toroid.reconfigure :default @11:03:16.84 Toroid.seek :clear @11:03:18.46 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:03:18.48 Pressure.coast @11:03:18.66 Sampled 15.2ml @11:03:20.74 -> Cmd.startProcessing @11:03:20.98 Plunge.seek :reagent @11:03:21.84 Sample loop closed. Ready to Deliver Reagents @11:03:21.85 Archive.seek :sealed @11:03:26.13 Archive.seek :evac @11:03:40.08 delay 1:00 @11:04:40.10 Archive.seek :clear @11:04:57.83 RNAlater soak for 10:00 @11:14:57.86 Archive.seek :tVent @11:15:00.44 delay 1 second @11:15:01.46 Archive.seek :evac @11:15:17.08 delay 10 seconds @11:15:27.11 Archive.seek :tVent @11:15:42.73 delay 1 second @11:15:43.75 Archive.seek :evac @11:15:59.37 delay 10 seconds @11:16:09.40 Sample preserved and evacuated @11:16:10.53 -> Cmd.stop @11:16:10.67 Carousel.seek 7 @11:16:10.84 Plunge.seek :locked @11:16:11.67 PinPort.seek :clear @11:16:11.81 Archive.seek :clear @11:16:11.94 Air.seek :clear @11:16:12.07 Lysis1.seek :clear @11:16:29.55 Archive.coast @11:16:29.61 Air.coast @11:16:29.66 Lysis1.coast @11:16:29.70 Lysis2.coast @11:16:29.75 Plunge.coast @11:16:29.81 SamplePump.coast @11:16:29.86 Toroid.coast @11:16:29.90 Carousel.coast @11:16:29.96 PinPort.coast @11:16:30.14 Gate.power :motor,:OFF @11:16:30.16 Gate.power :core,:OFF @11:16:30.18 Gate.power :analytic1,:OFF @11:16:30.20 Gate.power :analytic2,:OFF @11:16:30.22 Gate.power :analytic3,:OFF @11:16:30.24 Gate.power :main,:OFF @11:16:30.26 Safely stopped and ready to power off ##-- file closed 2019-08-27T15:32:19.207Z ##-- file opened 2019-08-27T15:32:19.211Z @11:32:19.39 -> showStatus @11:32:21.41 -> Cmd.loadCartridge -15 @11:32:21.55 Gate.power :main,:ON,limit to 5000mA @11:32:21.56 Gate.power :analytic1,:ON,limit to 5000mA @11:32:21.58 Gate.power :analytic2,:ON,limit to 5000mA @11:32:21.61 Gate.power :analytic3,:ON,limit to 5000mA @11:32:21.74 Powering core bus @11:32:24.02 SPR Module 1, FW Rev. B, FPGA Rev. C @11:32:24.16 Archive.configure :default @11:32:24.28 Air.configure :default @11:32:24.39 Lysis1.configure :default @11:32:24.49 Lysis2.configure :default @11:32:24.61 Plunge.configure :default @11:32:24.73 SamplePump.configure :default @11:32:24.85 ExtraPump.configure :default @11:32:24.97 Toroid.configure :default @11:32:25.12 Carousel.configure :default @11:32:25.24 Pressure.configure :default @11:32:25.34 PinPort.configure :default @11:32:25.40 All elf controllers running firmware version 4.58 @11:32:25.43 Gate.power :motor,:ON,limit to 5000mA @11:32:25.63 Archive.reconfigure :default @11:32:25.88 Archive.home.jog 0 @11:32:26.01 Toroid.reconfigure :default @11:32:26.09 Plunge.reconfigure :default @11:32:26.12 Lysis2.reconfigure :default @11:32:26.16 Air.reconfigure :default @11:32:26.20 PinPort.reconfigure :default @11:32:26.28 Lysis1.reconfigure :default @11:32:26.47 Plunge.home.jog 0 @11:32:26.53 Air.home.jog 0 @11:32:26.59 Lysis2.home.jog 0 @11:32:26.64 PinPort.home.jog 0 @11:32:26.70 Toroid.home.jog 0 @11:32:26.76 Lysis1.home.jog 0 @11:32:27.01 Archive.reconfigure :homing @11:32:27.15 Archive.jog 1381 @11:32:27.30 Toroid.reconfigure :homing @11:32:27.35 Plunge.reconfigure :homing @11:32:27.40 PinPort.reconfigure :homing @11:32:27.45 Lysis2.reconfigure :homing @11:32:27.48 Air.reconfigure :homing @11:32:27.68 Lysis2.jog 1429 @11:32:27.73 Air.jog 1410 @11:32:27.79 Plunge.jog 1538 @11:32:27.84 PinPort.jog -107 @11:32:27.91 Toroid.jog 625 @11:32:27.99 Lysis1.reconfigure :homing @11:32:28.07 PinPort.setRawPosition! 0 @11:32:28.12 PinPort.seek :home @11:32:28.19 Lysis1.jog 1399 @11:32:28.27 PinPort.reconfigure :default @11:32:28.35 Archive.setRawPosition! 50 @11:32:28.40 Archive.seek :home @11:32:28.68 Archive.reconfigure :default @11:32:28.84 Lysis2.setRawPosition! 50 @11:32:29.26 Lysis2.seek :home @11:32:29.36 Plunge.setRawPosition! 50 @11:32:29.38 Lysis1.setRawPosition! 50 @11:32:29.41 Air.setRawPosition! 50 @11:32:29.50 Plunge.seek :home @11:32:29.55 Lysis1.seek :home @11:32:29.59 Air.seek :home @11:32:29.79 Lysis2.reconfigure :default @11:32:29.89 Plunge.reconfigure :default @11:32:29.99 Lysis1.reconfigure :default @11:32:30.09 Air.reconfigure :default @11:32:30.11 Toroid.setRawPosition! 0 @11:32:30.20 Toroid.seek :home @11:32:30.30 Toroid.reconfigure :default @11:32:30.53 Toroid.seek :clear @11:32:32.15 Carousel.reconfigure :default @11:32:32.22 Carousel.home.jog 0 @11:32:32.71 Carousel.seek 7 @11:32:32.87 Selecting Cartridge 4 @11:32:33.12 Carousel.seek 4 @11:32:34.02 Lysis1.seek :locked @11:32:38.15 Plunge.seek :locked @11:32:38.27 Air.seek :locked @11:32:42.39 Powering cartridge bus @11:32:44.51 Gate.setIR 1750,5000,0,6 @11:32:44.64 Gate.setIR 0,5000,0,6 @11:32:44.69 Loaded Lyse-n-Go Cartridge @11:32:44.70 PuckHeater.configure HeatConfig @11:32:44.75 Gate.power :heat,:ON,limit to 5000mA @11:33:00.82 -> Cmd.startFiltering @11:33:01.05 Duration of filtering limited to 2:05:00 @11:33:01.30 Priming sample loop w/100ml, bypass w/10ml @11:33:01.32 SamplePump.setPosition! 0ml @11:33:01.36 Bypass.open @11:33:02.82 Intake.open @11:33:03.66 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:33:06.49 Vacuum tare is 52% of full scale @11:33:06.62 Pressure.reconfigure :default @11:33:06.68 Pressure.hold @11:33:06.84 SamplePump.reconfigure :prime @11:33:06.92 SamplePump.seek 10ml,1:21 @11:33:15.87 Pressure.coast @11:33:15.93 SamplePump.setPosition! 0ml @11:33:15.98 Exhaust.open @11:33:17.41 Bypass.close @11:33:18.94 Pressure.reconfigure :default @11:33:19.00 Pressure.hold @11:33:19.22 SamplePump.reconfigure :prime @11:33:19.29 SamplePump.seek 100ml,8:51 @11:35:16.82 Pressure.coast @11:35:16.88 Bypass.open @11:35:17.61 Intake.close @11:35:18.45 Toroid.reconfigure :hold @11:35:18.52 Toroid.seek :crack @11:35:19.31 Toroid.reconfigure :default @11:35:19.37 Toroid.seek :clear @11:35:20.93 Toroid.reconfigure :hold @11:35:21.00 Toroid.seek :cartridge @11:35:22.81 Toroid.reconfigure :default @11:35:22.87 Toroid.seek :clear @11:35:24.85 Pressurizing cartridge to 26psi above 12.8psia @11:35:24.86 SamplePump.setPosition! 0ml @11:35:24.90 Intake.open @11:35:25.62 Bypass.close @11:35:26.36 Exhaust.close @11:35:27.19 Pressure.reconfigure :default @11:35:27.25 Pressure.hold @11:35:27.41 SamplePump.reconfigure :charge @11:35:27.48 SamplePump.seek 2.3ml,42 seconds @11:35:39.13 Pressure.coast @11:35:39.18 Intake.close @11:35:39.98 Pressurized cartridge with 0.636ml to 26.2psi @11:35:39.99 Isolated @35.5psia (22.7psi) @11:35:40.00 Checking for cartridge leaks from 35.5psia @11:35:40.03 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @11:35:45.07 Cartridge pressure fell 1% to 35.3psia @11:35:45.15 Sampling 1000.0ml @11:35:45.16 SamplePump.setPosition! 0ml @11:35:45.21 Exhaust.open @11:35:45.96 Intake.open @11:35:46.83 Pressure.reconfigure :default @11:35:46.89 Pressure.hold @11:35:47.49 SamplePump.reconfigure :sample @11:35:47.57 SamplePump.seek 1000ml,1:23:53 @11:35:49.86 Cartridge 4 sampling 1/1000ml at 0.63ml/s with 23.6psi vacuum @11:36:49.86 Cartridge 4 sampling 32/1000ml at 0.50ml/s with 24.7psi vacuum @11:37:49.86 Cartridge 4 sampling 60/1000ml at 0.43ml/s with 26.5psi vacuum @11:38:49.86 Cartridge 4 sampling 85/1000ml at 0.40ml/s with 20.2psi vacuum @11:39:49.86 Cartridge 4 sampling 108/1000ml at 0.35ml/s with 27.5psi vacuum @11:40:49.86 Cartridge 4 sampling 128/1000ml at 0.34ml/s with 25.2psi vacuum @11:41:49.86 Cartridge 4 sampling 146/1000ml at 0.28ml/s with 26.5psi vacuum @11:42:49.86 Cartridge 4 sampling 162/1000ml at 0.20ml/s with 23.8psi vacuum @11:43:49.86 Cartridge 4 sampling 175/1000ml at 0.20ml/s with 25.7psi vacuum @11:44:49.86 Cartridge 4 sampling 187/1000ml at 0.20ml/s with 28.7psi vacuum @11:45:23.54 Flow averaged 0.34ml/s over 9:33 @11:45:23.57 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @11:45:24.27 Relieved excess vacuum in 4.3 seconds @11:45:24.29 SamplePump.seek 1000ml,1:07:45 @11:45:37.21 Flow estimate reduced to 0.27ml/s @11:45:37.25 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi @11:45:37.95 Relieved excess vacuum in 10.6 seconds @11:45:37.97 SamplePump.seek 1000ml,1:07:32 @11:45:49.86 Cartridge 4 sampling 198/1000ml at 0.20ml/s with 29.9psi vacuum @11:45:54.05 Flow averaged 0.27ml/s over 12 seconds @11:45:54.08 Waiting up to 17 seconds for vacuum to reduce from 29.8 to 18.0psi @11:45:54.78 Relieved excess vacuum in 4.8 seconds @11:45:54.81 SamplePump.seek 1000ml,1:07:16 @11:46:01.47 Flow estimate reduced to 0.21ml/s @11:46:01.50 Waiting up to 16 seconds for vacuum to reduce from 29.1 to 18.0psi @11:46:02.20 Relieved excess vacuum in 4.3 seconds @11:46:02.22 SamplePump.seek 1000ml,1:07:09 @11:46:08.68 Flow estimate reduced to 0.17ml/s @11:46:08.69 Intake.close @11:46:08.75 Exhaust.close @11:46:08.92 Toroid.reconfigure :hold @11:46:08.99 Toroid.seek :bypass @11:46:10.61 Toroid.reconfigure :default @11:46:10.68 Toroid.seek :clear @11:46:12.30 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:46:12.32 Pressure.coast @11:46:12.51 Sampled 201.8ml @11:46:14.43 -> Cmd.startProcessing @11:46:16.30 SPR.command \"read_sequences\" @11:46:16.33 SPR.command \"stats_on\" @11:46:16.37 SPR.command \"start 0 1\" @11:46:18.38 Begun log /var/log/esp/spr19aug2711h.spr @11:46:19.09 Handoff.select :waste @11:46:19.27 Archive.seek :empty @11:46:26.47 Syringe tare is 21% of full scale @11:46:36.99 PinPort.seek :cartridge @11:46:41.55 Lysis1.seek 1350ul @11:46:44.02 Lysis2.seek 1700ul @11:46:47.12 Plunge.seek :reagent @11:46:47.97 Evacuating puck with filter bubble point of 50psi @11:46:47.99 PinPort.seek :cartridge @11:46:48.07 Handoff.select :waste @11:46:55.40 Air pumping 1 strokes to reach 8psi @11:46:55.42 Air.reconfigure :gulp @11:46:55.51 Air.seek :tVent @11:46:57.32 0.0psi after gulp @11:46:57.33 Air stroke #1 of 1 @11:46:57.35 Air.reconfigure :pressurize @11:46:57.43 Air.seek :pressurized @11:47:10.27 0.0psi after pressurize @11:47:10.28 Air.reconfigure :compress @11:47:10.37 Air.seek :evac @11:47:13.03 0.3psi after compress @11:47:26.99 0.4psi after compress (stable) @11:47:27.11 at 0.5psi Opened to waste @11:47:27.12 Handoff.select :blocked @11:47:27.29 Air pumping 10 strokes to reach 50psi @11:47:27.31 Air.reconfigure :gulp @11:47:27.39 Air.seek :tVent @11:47:37.27 11.2psi after gulp @11:47:37.28 Air stroke #1 of 10 @11:47:37.30 Air.reconfigure :pressurize @11:47:37.37 Air.seek :pressurized @11:47:50.20 14.9psi after pressurize @11:47:50.22 Air.reconfigure :compress @11:47:50.29 Air.seek :evac @11:47:52.97 17.7psi after compress @11:48:19.60 37.6psi after compress (stable) @11:48:19.62 Air.reconfigure :gulp @11:48:19.71 Air.seek :tVent @11:48:29.60 38.2psi after gulp @11:48:29.61 Air stroke #2 of 10 @11:48:29.63 Air.reconfigure :pressurize @11:48:29.71 Air.seek :pressurized @11:48:42.54 36.3psi after pressurize @11:48:42.55 Air.reconfigure :compress @11:48:42.64 Air.seek :evac @11:48:45.32 37.1psi after compress @11:48:50.87 Reached 50.0 of 50.0psi after pumping 4672ul (3.00 strokes) @11:48:50.88 Handoff.select :waste @11:49:25.75 delay 30 seconds @11:49:55.77 Handoff.select :blocked @11:50:20.75 Handoff.select :waste @11:50:20.88 PSI at @4.71psi at evacEnd @11:50:20.89 Handoff.select :blocked @11:50:21.01 Air.reconfigure :gulp @11:50:21.14 Air.seek :tVent @11:50:38.26 Stable @1.7psi after Handoff blocked @11:50:38.34 Air pumping 10 strokes to reach 32psi @11:50:38.36 Air stroke #1 of 10 @11:50:38.38 Air.reconfigure :pressurize @11:50:38.46 Air.seek :pressurized @11:50:51.29 3.4psi after pressurize @11:50:51.30 Air.reconfigure :compress @11:50:51.39 Air.seek :evac @11:50:54.05 6.2psi after compress @11:51:16.57 15.5psi after compress (stable) @11:51:16.59 Air.reconfigure :gulp @11:51:16.68 Air.seek :tVent @11:51:26.56 15.9psi after gulp @11:51:26.57 Air stroke #2 of 10 @11:51:26.60 Air.reconfigure :pressurize @11:51:26.67 Air.seek :pressurized @11:51:39.51 15.8psi after pressurize @11:51:39.53 Air.reconfigure :compress @11:51:39.60 Air.seek :evac @11:51:42.27 18.5psi after compress @11:52:01.13 26.1psi after compress (stable) @11:52:01.15 Air.reconfigure :gulp @11:52:01.22 Air.seek :tVent @11:52:11.11 26.5psi after gulp @11:52:11.12 Air stroke #3 of 10 @11:52:11.14 Air.reconfigure :pressurize @11:52:11.21 Air.seek :pressurized @11:52:24.04 26.4psi after pressurize @11:52:24.06 Air.reconfigure :compress @11:52:24.14 Air.seek :evac @11:52:26.81 28.4psi after compress @11:52:28.56 Reached 31.8 of 31.7psi after pumping 6230ul (4.00 strokes) @11:52:28.57 Handoff.select :waste @11:52:36.48 delay 30 seconds @11:53:06.50 Handoff.select :blocked @11:53:31.88 Handoff.select :waste @11:53:31.99 PSI at @0.99psi at evacEnd @11:53:32.01 Air.reconfigure :default @11:53:32.12 Air.seek :fVent @11:53:33.56 delay 5 seconds @11:53:38.58 Seawater evacuated, Presure above/below filter released. Lysis begins @11:53:38.65 Lysis1.reconfigure :slow @11:53:38.72 Lysis1.seek 1050ul @11:54:08.72 delay 5 seconds @11:54:13.75 Handoff.select :blocked @11:54:13.91 Lysis1.seek 450ul @11:55:13.85 delay 3 seconds @11:55:16.92 Air.reconfigure to mix into 1.37806 parts Lysis1 @11:55:17.36 Air.seek :evac @11:55:17.40 Lysis1.seek 300ul @11:55:32.51 Air.reconfigure :default @11:55:32.57 Air.seek :full @11:55:46.79 Lysis1.seek :empty @11:56:16.90 Lysis Delivered. Syringe pressure @23.6psi @11:56:16.95 PuckHeater.seek 84.5C @11:56:55.97 PuckHeater.hold 15:00,84.5C @12:11:54.14 Lysis complete. Syringe pressure @31.4psi @12:11:54.15 Handoff.select :blocked @12:11:54.25 Lysis1.reconfigure :halfSpeed @12:11:54.32 Lysis1.seek 200ul @12:11:58.09 delay 1 second @12:11:59.11 Lysis1.seek :empty @12:12:02.94 Initial AirS pressure @31.6psi @12:12:03.00 Lysis1.reconfigure :extract @12:12:03.09 Lysis1.seek 900ul,10:00 @12:12:04.25 Air pumping 10 strokes to reach 21psi @12:12:04.26 Air stroke #1 of 10 @12:12:04.28 Air.reconfigure :pressurize @12:12:04.36 Air.seek :pressurized @12:12:17.19 16.2psi after pressurize @12:12:17.20 Air.reconfigure :compress @12:12:17.28 Air.seek :evac @12:12:19.95 17.5psi after compress @12:12:34.25 15.6psi after compress (stable) @12:12:34.27 Air.reconfigure :gulp @12:12:34.35 Air.seek :tVent @12:12:44.26 15.5psi after gulp @12:12:44.27 Air stroke #2 of 10 @12:12:44.29 Air.reconfigure :pressurize @12:12:44.37 Air.seek :pressurized @12:12:57.20 16.2psi after pressurize @12:12:57.22 Air.reconfigure :compress @12:12:57.29 Air.seek :evac @12:13:00.03 17.3psi after compress @12:13:12.17 Air.reconfigure :default @12:13:12.24 Air.hold @12:13:12.42 Lysis1 at 900ul @15.9psi @12:13:12.43 Lysis1.reconfigure :default @12:13:12.51 Lysis1.seek 925ul @12:13:13.30 Lysis1 at 925ul, bottom @14.7psi after recovery @12:13:14.41 Lysis1 at 925ul, bottom @16.0psi @12:13:14.43 Air.reconfigure :halfSpeed @12:13:14.52 Air.seek :fVent @12:13:16.77 Puck Top Vented, bottom @5.1psi @12:13:16.78 Handoff.select :waste @12:13:16.90 delay 5 seconds @12:13:21.94 delay 5 seconds @12:13:26.96 Handoff.select SPR @12:13:27.07 SPR.command \"start 2 14\" @12:13:27.92 delay 10 seconds @12:13:37.94 SPR.command \"start 2 0\" @12:13:41.17 Waiting up to 11:00 for SPR to enter state 1 @12:22:13.25 SPR.command \"hold_on\" @12:22:14.63 SPR Dilution 1 of 1 -- Running Experiment 2 @12:22:14.64 Lysis2.reconfigure :DAmix @12:22:14.76 Lysis1.reconfigure :DAmix @12:22:15.11 Lysis2.seek 1450ul @12:22:15.19 Lysis1.seek 675ul @12:22:50.54 Lysis2.seek 1150ul @12:22:50.58 Lysis1.seek 375ul @12:23:32.57 volL1 = 300.0 and volL2 = 300.0 @12:23:32.59 delay 15 seconds @12:23:47.62 SPR.command \"hold_off\" @12:23:50.28 Waiting up to 25:00 for SPR to enter state 7 @12:35:26.80 SPR Analysis has Completed @12:35:26.81 Handoff.select :waste @12:35:27.72 Ended log /var/log/esp/spr19aug2711h.spr @12:35:27.73 SPR.command \"stats_off\" @12:35:29.44 -> Cmd.stop @12:35:29.53 Handoff.select :close @12:35:29.66 Gate.power :heat,:OFF @12:35:29.68 Gate.power :cartridge,:OFF @12:35:29.76 Carousel.seek 4 @12:35:29.95 Plunge.seek :locked @12:35:30.79 PinPort.seek :clear @12:35:35.42 Archive.seek :clear @12:35:35.55 Air.reconfigure :default @12:35:35.69 Lysis1.reconfigure :default @12:35:35.80 Air.seek :clear @12:35:35.86 Lysis1.seek :clear @12:35:35.95 Lysis2.reconfigure :default @12:35:36.02 Lysis2.seek :clear @12:35:53.16 Archive.coast @12:35:53.21 Air.coast @12:35:53.26 Lysis1.coast @12:35:53.31 Lysis2.coast @12:35:53.36 Plunge.coast @12:35:53.42 SamplePump.coast @12:35:53.47 Toroid.coast @12:35:53.52 Carousel.coast @12:35:53.57 PinPort.coast @12:35:53.76 Gate.power :motor,:OFF @12:35:53.78 Gate.power :core,:OFF @12:35:53.80 Gate.power :analytic1,:OFF @12:35:53.82 Gate.power :analytic2,:OFF @12:35:53.84 Gate.power :analytic3,:OFF @12:35:53.86 Gate.power :main,:OFF @12:35:53.87 Safely stopped and ready to power off ##-- file closed 2019-08-27T19:12:03.945Z ##-- file opened 2019-08-27T19:12:03.947Z @15:12:03.90 -> showStatus @15:12:05.98 -> Cmd.loadCartridge -4 @15:12:06.12 Gate.power :main,:ON,limit to 5000mA @15:12:06.13 Gate.power :analytic1,:ON,limit to 5000mA @15:12:06.15 Gate.power :analytic2,:ON,limit to 5000mA @15:12:06.17 Gate.power :analytic3,:ON,limit to 5000mA @15:12:06.30 Powering core bus @15:12:08.58 SPR Module 1, FW Rev. B, FPGA Rev. C @15:12:08.71 Archive.configure :default @15:12:08.83 Air.configure :default @15:12:08.94 Lysis1.configure :default @15:12:09.04 Lysis2.configure :default @15:12:09.16 Plunge.configure :default @15:12:09.27 SamplePump.configure :default @15:12:09.39 ExtraPump.configure :default @15:12:09.51 Toroid.configure :default @15:12:09.62 Carousel.configure :default @15:12:09.74 Pressure.configure :default @15:12:09.85 PinPort.configure :default @15:12:09.91 All elf controllers running firmware version 4.58 @15:12:09.93 Gate.power :motor,:ON,limit to 5000mA @15:12:10.15 Archive.reconfigure :default @15:12:10.37 Archive.home.jog 0 @15:12:10.52 Toroid.reconfigure :default @15:12:10.59 Plunge.reconfigure :default @15:12:10.63 Lysis2.reconfigure :default @15:12:10.66 Air.reconfigure :default @15:12:10.69 PinPort.reconfigure :default @15:12:10.73 Lysis1.reconfigure :default @15:12:11.00 Air.home.jog 0 @15:12:11.05 Plunge.home.jog 0 @15:12:11.11 Toroid.home.jog 0 @15:12:11.17 PinPort.home.jog 0 @15:12:11.22 Lysis1.home.jog 0 @15:12:11.28 Lysis2.home.jog 0 @15:12:11.55 Archive.reconfigure :homing @15:12:11.86 Toroid.reconfigure :homing @15:12:11.88 Plunge.reconfigure :homing @15:12:11.91 Air.reconfigure :homing @15:12:11.95 PinPort.reconfigure :homing @15:12:11.99 Lysis1.reconfigure :homing @15:12:12.03 Lysis2.reconfigure :homing @15:12:12.24 PinPort.jog -107 @15:12:12.29 Lysis1.jog 1401 @15:12:12.35 Toroid.jog 621 @15:12:12.40 Air.jog 1412 @15:12:12.47 Plunge.jog 1539 @15:12:12.52 Archive.jog 1381 @15:12:12.59 Lysis2.jog 1426 @15:12:12.66 PinPort.setRawPosition! 0 @15:12:12.70 PinPort.seek :home @15:12:12.79 PinPort.reconfigure :default @15:12:13.45 Lysis1.setRawPosition! 50 @15:12:13.49 Lysis1.seek :home @15:12:13.97 Plunge.setRawPosition! 50 @15:12:14.00 Lysis2.setRawPosition! 50 @15:12:14.02 Air.setRawPosition! 50 @15:12:14.04 Archive.setRawPosition! 50 @15:12:14.16 Plunge.seek :home @15:12:14.18 Lysis2.seek :home @15:12:14.36 Archive.seek :home @15:12:14.39 Air.seek :home @15:12:14.55 Toroid.setRawPosition! 0 @15:12:14.58 Plunge.reconfigure :default @15:12:14.60 Lysis1.reconfigure :default @15:12:14.73 Toroid.seek :home @15:12:14.90 Toroid.reconfigure :default @15:12:14.92 Lysis2.reconfigure :default @15:12:14.95 Air.reconfigure :default @15:12:14.98 Archive.reconfigure :default @15:12:15.47 Toroid.seek :clear @15:12:17.09 Carousel.reconfigure :default @15:12:17.16 Carousel.home.jog 0 @15:12:17.66 Carousel.seek 4 @15:12:17.81 Selecting Cartridge 5 @15:12:18.06 Carousel.seek 5 @15:12:18.54 Lysis1.seek :locked @15:12:20.58 Slide::Error in LRAUV -- Lysis Syringe #1 (LyS1) positionErr at 1790ul (sensed 1821ul) @15:12:42.86 -> Cmd.stop @15:12:43.00 Carousel.seek 5 @15:12:43.17 Slide::Error in LRAUV -- Lysis Syringe #1 (LyS1) Not yet Homed! @15:12:43.19 Archive.coast @15:12:43.23 Air.coast @15:12:43.28 Lysis1.coast @15:12:43.33 Lysis2.coast @15:12:43.38 Plunge.coast @15:12:43.43 Toroid.coast @15:12:43.47 Carousel.coast @15:12:43.52 PinPort.coast @15:12:43.70 Gate.power :motor,:OFF @15:12:43.72 Gate.power :core,:OFF @15:12:43.74 Gate.power :analytic1,:OFF @15:12:43.76 Gate.power :analytic2,:OFF @15:12:43.78 Gate.power :analytic3,:OFF @15:12:43.80 Gate.power :main,:OFF @15:12:43.82 ready to power off ##-- file closed 2019-08-27T19:56:59.694Z ##-- file opened 2019-08-27T19:56:59.695Z @15:57:00.02 -> showStatus @15:57:02.14 -> Cmd.loadCartridge -4 @15:57:02.28 Gate.power :main,:ON,limit to 5000mA @15:57:02.29 Gate.power :analytic1,:ON,limit to 5000mA @15:57:02.31 Gate.power :analytic2,:ON,limit to 5000mA @15:57:02.35 Gate.power :analytic3,:ON,limit to 5000mA @15:57:02.48 Powering core bus @15:57:04.76 SPR Module 1, FW Rev. B, FPGA Rev. C @15:57:04.90 Archive.configure :default @15:57:05.02 Air.configure :default @15:57:05.12 Lysis1.configure :default @15:57:05.22 Lysis2.configure :default @15:57:05.34 Plunge.configure :default @15:57:05.46 SamplePump.configure :default @15:57:05.58 ExtraPump.configure :default @15:57:05.70 Toroid.configure :default @15:57:05.82 Carousel.configure :default @15:57:05.94 Pressure.configure :default @15:57:06.05 PinPort.configure :default @15:57:06.10 All elf controllers running firmware version 4.58 @15:57:06.13 Gate.power :motor,:ON,limit to 5000mA @15:57:06.33 Archive.reconfigure :default @15:57:06.59 Archive.home.jog 0 @15:57:06.73 Toroid.reconfigure :default @15:57:06.81 Plunge.reconfigure :default @15:57:06.85 Lysis1.reconfigure :default @15:57:06.91 Air.reconfigure :default @15:57:06.95 PinPort.reconfigure :default @15:57:06.99 Lysis2.reconfigure :default @15:57:07.22 Lysis1.home.jog 0 @15:57:07.27 PinPort.home.jog 0 @15:57:07.32 Plunge.home.jog 0 @15:57:07.39 Air.home.jog 0 @15:57:07.45 Lysis2.home.jog 0 @15:57:07.51 Toroid.home.jog 0 @15:57:07.81 Archive.reconfigure :homing @15:57:08.02 Toroid.reconfigure :homing @15:57:08.06 Plunge.reconfigure :homing @15:57:08.09 Air.reconfigure :homing @15:57:08.13 PinPort.reconfigure :homing @15:57:08.17 Lysis1.reconfigure :homing @15:57:08.20 Lysis2.reconfigure :homing @15:57:08.43 Lysis2.jog 1427 @15:57:08.48 Plunge.jog 1531 @15:57:08.53 Air.jog 2156 @15:57:08.59 Lysis1.jog 2127 @15:57:08.65 Toroid.jog 630 @15:57:08.70 Archive.jog 1379 @15:57:08.76 PinPort.jog -108 @15:57:08.99 PinPort.setRawPosition! 0 @15:57:09.03 PinPort.seek :home @15:57:09.12 PinPort.reconfigure :default @15:57:09.59 Lysis2.setRawPosition! 50 @15:57:09.63 Lysis2.seek :home @15:57:09.72 Plunge.setRawPosition! 50 @15:57:10.15 Plunge.seek :home @15:57:10.26 Lysis2.reconfigure :default @15:57:10.27 Lysis1.setRawPosition! 50 @15:57:10.29 Air.setRawPosition! 50 @15:57:10.33 Archive.setRawPosition! 50 @15:57:10.45 Lysis1.seek :home @15:57:10.49 Air.seek :home @15:57:10.51 Archive.seek :home @15:57:10.73 Plunge.reconfigure :default @15:57:10.82 Toroid.setRawPosition! 0 @15:57:10.87 Toroid.seek :home @15:57:11.00 Toroid.reconfigure :default @15:57:11.04 Lysis1.reconfigure :default @15:57:11.07 Air.reconfigure :default @15:57:11.10 Archive.reconfigure :default @15:57:11.57 Toroid.seek :clear @15:57:13.19 Carousel.reconfigure :default @15:57:13.26 Carousel.home.jog 0 @15:57:13.75 Carousel.seek 5 @15:57:13.91 Selecting Cartridge 5 @15:57:14.16 Carousel.seek 5 @15:57:14.34 Lysis1.seek :locked @15:57:18.48 Plunge.seek :locked @15:57:18.60 Air.seek :locked @15:57:22.72 Powering cartridge bus @15:57:24.84 Gate.setIR 1750,5000,0,6 @15:57:24.97 Gate.setIR 0,5000,0,6 @15:57:25.01 Loaded Archive Cartridge Gate.power :cartridge,:OFF @15:57:41.20 -> Cmd.startFiltering @15:57:41.43 Duration of filtering limited to 2:05:00 @15:57:41.69 Priming sample loop w/100ml, bypass w/10ml @15:57:41.70 SamplePump.setPosition! 0ml @15:57:41.74 Bypass.open @15:57:43.25 Intake.open @15:57:44.06 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:57:46.90 Vacuum tare is 52% of full scale @15:57:47.03 Pressure.reconfigure :default @15:57:47.09 Pressure.hold @15:57:47.25 SamplePump.reconfigure :prime @15:57:47.33 SamplePump.seek 10ml,1:21 @15:57:56.28 Pressure.coast @15:57:56.33 SamplePump.setPosition! 0ml @15:57:56.38 Exhaust.open @15:57:57.79 Bypass.close @15:57:59.32 Pressure.reconfigure :default @15:57:59.38 Pressure.hold @15:57:59.60 SamplePump.reconfigure :prime @15:57:59.68 SamplePump.seek 100ml,8:51 @15:59:56.84 Pressure.coast @15:59:56.90 Bypass.open @15:59:57.63 Intake.close @15:59:58.45 Toroid.reconfigure :hold @15:59:58.51 Toroid.seek :crack @15:59:59.31 Toroid.reconfigure :default @15:59:59.37 Toroid.seek :clear @16:00:00.93 Toroid.reconfigure :hold @16:00:01.00 Toroid.seek :cartridge @16:00:02.81 Toroid.reconfigure :default @16:00:02.87 Toroid.seek :clear @16:00:04.85 Pressurizing cartridge to 26psi above 14.1psia @16:00:04.86 SamplePump.setPosition! 0ml @16:00:04.90 Intake.open @16:00:05.66 Bypass.close @16:00:06.43 Exhaust.close @16:00:07.24 Pressure.reconfigure :default @16:00:07.29 Pressure.hold @16:00:07.46 SamplePump.reconfigure :charge @16:00:07.53 SamplePump.seek 2.3ml,42 seconds @16:00:16.03 Pressure.coast @16:00:16.08 Intake.close @16:00:16.93 Pressurized cartridge with 0.337ml to 26.1psi @16:00:16.94 Isolated @36.4psia (22.3psi) @16:00:16.95 Checking for cartridge leaks from 36.4psia @16:00:16.97 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @16:00:22.02 Cartridge pressure fell 2% to 35.7psia @16:00:22.10 Sampling 1000.0ml @16:00:22.11 SamplePump.setPosition! 0ml @16:00:22.16 Exhaust.open @16:00:22.88 Intake.open @16:00:23.77 Pressure.reconfigure :default @16:00:23.83 Pressure.hold @16:00:24.43 SamplePump.reconfigure :sample @16:00:24.51 SamplePump.seek 1000ml,1:23:53 @16:00:26.81 Cartridge 5 sampling 1/1000ml at 0.36ml/s with 24.8psi vacuum @16:01:26.81 Cartridge 5 sampling 19/1000ml at 0.20ml/s with 26.4psi vacuum @16:01:38.42 Flow averaged 0.29ml/s over 1:13 @16:01:38.45 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @16:01:39.45 Relieved excess vacuum in 2.5 seconds @16:01:39.47 SamplePump.seek 1000ml,1:22:08 @16:01:41.87 Flow estimate reduced to 0.23ml/s @16:01:41.90 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @16:01:42.90 Relieved excess vacuum in 3.3 seconds @16:01:42.92 SamplePump.seek 1000ml,1:22:06 @16:01:44.85 Flow estimate reduced to 0.19ml/s @16:01:44.88 Intake.close @16:01:44.98 Exhaust.close @16:01:45.14 Toroid.reconfigure :hold @16:01:45.21 Toroid.seek :bypass @16:01:46.83 Toroid.reconfigure :default @16:01:46.90 Toroid.seek :clear @16:01:48.52 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @16:01:48.54 Pressure.coast @16:01:48.72 Sampled 21.8ml @16:01:50.93 -> Cmd.startProcessing @16:01:51.17 Plunge.seek :reagent @16:01:52.04 Sample loop closed. Ready to Deliver Reagents @16:01:52.05 Archive.seek :sealed @16:01:56.33 Archive.seek :evac @16:02:10.27 delay 1:00 @16:03:10.30 Archive.seek :clear @16:03:28.01 RNAlater soak for 10:00 @16:13:28.03 Archive.seek :tVent @16:13:30.61 delay 1 second @16:13:31.63 Archive.seek :evac @16:13:47.25 delay 10 seconds @16:13:57.28 Archive.seek :tVent @16:14:12.91 delay 1 second @16:14:13.93 Archive.seek :evac @16:14:29.54 delay 10 seconds @16:14:39.57 Sample preserved and evacuated @16:14:40.53 -> Cmd.stop @16:14:40.67 Carousel.seek 5 @16:14:40.84 Plunge.seek :locked @16:14:41.67 PinPort.seek :clear @16:14:41.81 Archive.seek :clear @16:14:41.94 Air.seek :clear @16:14:42.06 Lysis1.seek :clear @16:14:59.55 Archive.coast @16:14:59.60 Air.coast @16:14:59.65 Lysis1.coast @16:14:59.70 Lysis2.coast @16:14:59.75 Plunge.coast @16:14:59.80 SamplePump.coast @16:14:59.85 Toroid.coast @16:14:59.90 Carousel.coast @16:14:59.95 PinPort.coast @16:15:00.14 Gate.power :motor,:OFF @16:15:00.15 Gate.power :core,:OFF @16:15:00.18 Gate.power :analytic1,:OFF @16:15:00.20 Gate.power :analytic2,:OFF @16:15:00.22 Gate.power :analytic3,:OFF @16:15:00.24 Gate.power :main,:OFF @16:15:00.26 Safely stopped and ready to power off ##-- file closed 2019-08-27T20:30:49.052Z ##-- file opened 2019-08-27T20:30:49.056Z @16:30:49.11 -> showStatus @16:30:51.13 -> Cmd.loadCartridge -15 @16:30:51.27 Gate.power :main,:ON,limit to 5000mA @16:30:51.29 Gate.power :analytic1,:ON,limit to 5000mA @16:30:51.32 Gate.power :analytic2,:ON,limit to 5000mA @16:30:51.34 Gate.power :analytic3,:ON,limit to 5000mA @16:30:51.47 Powering core bus @16:30:53.75 SPR Module 1, FW Rev. B, FPGA Rev. C @16:30:53.93 Archive.configure :default @16:30:54.04 Air.configure :default @16:30:54.14 Lysis1.configure :default @16:30:54.25 Lysis2.configure :default @16:30:54.36 Plunge.configure :default @16:30:54.48 SamplePump.configure :default @16:30:54.60 ExtraPump.configure :default @16:30:54.72 Toroid.configure :default @16:30:54.84 Carousel.configure :default @16:30:54.95 Pressure.configure :default @16:30:55.05 PinPort.configure :default @16:30:55.11 All elf controllers running firmware version 4.58 @16:30:55.14 Gate.power :motor,:ON,limit to 5000mA @16:30:55.38 Archive.reconfigure :default @16:30:55.58 Archive.home.jog 0 @16:30:55.71 Toroid.reconfigure :default @16:30:55.79 Plunge.reconfigure :default @16:30:55.83 Lysis1.reconfigure :default @16:30:55.88 Air.reconfigure :default @16:30:55.93 Lysis2.reconfigure :default @16:30:55.97 PinPort.reconfigure :default @16:30:56.20 Lysis2.home.jog 0 @16:30:56.25 Toroid.home.jog 0 @16:30:56.31 Plunge.home.jog 0 @16:30:56.37 Lysis1.home.jog 0 @16:30:56.43 Air.home.jog 0 @16:30:56.48 PinPort.home.jog 0 @16:30:56.78 Archive.reconfigure :homing @16:30:56.91 Archive.jog 1371 @16:30:57.07 Toroid.reconfigure :homing @16:30:57.11 Plunge.reconfigure :homing @16:30:57.14 Air.reconfigure :homing @16:30:57.17 PinPort.reconfigure :homing @16:30:57.20 Lysis1.reconfigure :homing @16:30:57.26 Lysis2.reconfigure :homing @16:30:57.46 Lysis1.jog 1404 @16:30:57.51 Toroid.jog 629 @16:30:57.57 PinPort.jog -107 @16:30:57.63 Plunge.jog 1534 @16:30:57.68 Air.jog 1400 @16:30:57.74 Lysis2.jog 1428 @16:30:57.82 PinPort.setRawPosition! 0 @16:30:57.86 PinPort.seek :home @16:30:57.95 PinPort.reconfigure :default @16:30:58.05 Archive.setRawPosition! 50 @16:30:58.42 Archive.seek :home @16:30:58.61 Lysis1.setRawPosition! 50 @16:30:58.66 Lysis1.seek :home @16:30:58.75 Archive.reconfigure :default @16:30:58.84 Plunge.setRawPosition! 50 @16:30:58.86 Air.setRawPosition! 50 @16:30:58.96 Plunge.seek :home @16:30:58.99 Air.seek :home @16:30:59.12 Lysis2.setRawPosition! 50 @16:30:59.14 Lysis1.reconfigure :default @16:30:59.22 Lysis2.seek :home @16:30:59.39 Plunge.reconfigure :default @16:30:59.42 Air.reconfigure :default @16:30:59.56 Lysis2.reconfigure :default @16:30:59.65 Toroid.setRawPosition! 0 @16:30:59.70 Toroid.seek :home @16:30:59.79 Toroid.reconfigure :default @16:30:59.94 Toroid.seek :clear @16:31:01.56 Carousel.reconfigure :default @16:31:01.63 Carousel.home.jog 0 @16:31:02.13 Carousel.seek 5 @16:31:02.29 Selecting Cartridge 3 @16:31:02.53 Carousel.seek 3 @16:31:03.22 Lysis1.seek :locked @16:31:07.36 Plunge.seek :locked @16:31:07.48 Air.seek :locked @16:31:11.61 Powering cartridge bus @16:31:13.73 Gate.setIR 1750,5000,0,6 @16:31:13.86 Gate.setIR 0,5000,0,6 @16:31:13.92 Loaded Lyse-n-Go Cartridge @16:31:13.94 PuckHeater.configure HeatConfig @16:31:13.99 Gate.power :heat,:ON,limit to 5000mA @16:31:30.19 -> Cmd.startFiltering @16:31:30.42 Duration of filtering limited to 2:05:00 @16:31:30.67 Priming sample loop w/100ml, bypass w/10ml @16:31:30.68 SamplePump.setPosition! 0ml @16:31:30.72 Bypass.open @16:31:32.23 Intake.open @16:31:33.02 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:31:35.86 Vacuum tare is 52% of full scale @16:31:35.99 Pressure.reconfigure :default @16:31:36.05 Pressure.hold @16:31:36.21 SamplePump.reconfigure :prime @16:31:36.28 SamplePump.seek 10ml,1:21 @16:31:45.24 Pressure.coast @16:31:45.29 SamplePump.setPosition! 0ml @16:31:45.34 Exhaust.open @16:31:46.78 Bypass.close @16:31:48.30 Pressure.hold @16:31:48.53 SamplePump.reconfigure :prime @16:31:48.60 SamplePump.seek 100ml,8:51 @16:33:45.92 Pressure.coast @16:33:45.98 Bypass.open @16:33:46.68 Intake.close @16:33:47.52 Toroid.reconfigure :hold @16:33:47.58 Toroid.seek :crack @16:33:48.38 Toroid.reconfigure :default @16:33:48.44 Toroid.seek :clear @16:33:50.00 Toroid.reconfigure :hold @16:33:50.07 Toroid.seek :cartridge @16:33:51.88 Toroid.reconfigure :default @16:33:51.94 Toroid.seek :clear @16:33:53.90 Pressurizing cartridge to 26psi above 12.4psia @16:33:53.91 SamplePump.setPosition! 0ml @16:33:53.96 Intake.open @16:33:54.70 Bypass.close @16:33:55.43 Exhaust.close @16:33:56.22 Pressure.reconfigure :default @16:33:56.27 Pressure.hold @16:33:56.44 SamplePump.reconfigure :charge @16:33:56.51 SamplePump.seek 2.3ml,42 seconds @16:34:05.99 Pressure.coast @16:34:06.04 Intake.close @16:34:06.86 Pressurized cartridge with 0.420ml to 26.0psi @16:34:06.87 Isolated @35.5psia (23.1psi) @16:34:06.88 Checking for cartridge leaks from 35.5psia @16:34:06.91 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @16:34:11.96 Cartridge pressure fell 1% to 35.4psia @16:34:12.04 Sampling 1000.0ml @16:34:12.05 SamplePump.setPosition! 0ml @16:34:12.10 Exhaust.open @16:34:12.80 Intake.open @16:34:14.07 Pressure.reconfigure :default @16:34:14.13 Pressure.hold @16:34:14.35 SamplePump.reconfigure :sample @16:34:14.43 SamplePump.seek 1000ml,1:23:53 @16:34:16.72 Cartridge 3 sampling 1/1000ml at 0.54ml/s with 28.0psi vacuum @16:35:16.73 Cartridge 3 sampling 30/1000ml at 0.46ml/s with 22.4psi vacuum @16:36:16.72 Cartridge 3 sampling 57/1000ml at 0.41ml/s with 25.3psi vacuum @16:37:16.73 Cartridge 3 sampling 81/1000ml at 0.38ml/s with 23.1psi vacuum @16:38:16.73 Cartridge 3 sampling 103/1000ml at 0.34ml/s with 22.8psi vacuum @16:39:16.73 Cartridge 3 sampling 124/1000ml at 0.35ml/s with 25.4psi vacuum @16:40:16.73 Cartridge 3 sampling 143/1000ml at 0.32ml/s with 23.5psi vacuum @16:41:16.72 Cartridge 3 sampling 161/1000ml at 0.24ml/s with 25.1psi vacuum @16:42:16.73 Cartridge 3 sampling 175/1000ml at 0.23ml/s with 25.6psi vacuum @16:43:16.73 Cartridge 3 sampling 188/1000ml at 0.21ml/s with 25.0psi vacuum @16:44:16.73 Cartridge 3 sampling 200/1000ml at 0.20ml/s with 26.6psi vacuum @16:44:43.56 Flow averaged 0.33ml/s over 10:23 @16:44:43.59 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @16:44:44.29 Relieved excess vacuum in 7.4 seconds @16:44:44.31 SamplePump.seek 1000ml,1:06:45 @16:44:54.09 Flow estimate reduced to 0.26ml/s @16:44:54.13 Waiting up to 12 seconds for vacuum to reduce from 27.4 to 18.0psi @16:44:54.82 Relieved excess vacuum in 7.4 seconds @16:44:54.84 SamplePump.seek 1000ml,1:06:35 @16:45:04.58 Flow estimate reduced to 0.21ml/s @16:45:04.62 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @16:45:05.32 Relieved excess vacuum in 7.4 seconds @16:45:05.34 SamplePump.seek 1000ml,1:06:25 @16:45:15.12 Flow estimate reduced to 0.17ml/s @16:45:15.13 Intake.close @16:45:15.19 Exhaust.close @16:45:15.35 Toroid.reconfigure :hold @16:45:15.42 Toroid.seek :bypass @16:45:16.72 Cartridge 3 sampling 211/1000ml at 0.00ml/s with 1.4psi vacuum @16:45:17.04 Toroid.reconfigure :default @16:45:17.11 Toroid.seek :clear @16:45:18.73 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @16:45:18.75 Pressure.coast @16:45:18.93 Sampled 211.3ml @16:45:20.86 -> Cmd.startProcessing @16:45:22.71 SPR.command \"read_sequences\" @16:45:22.87 SPR.command \"stats_on\" @16:45:22.91 SPR.command \"start 0 1\" @16:45:24.91 Begun log /var/log/esp/spr19aug2716h.spr @16:45:25.72 Handoff.select :waste @16:45:25.91 Archive.seek :empty @16:45:33.12 Syringe tare is 21% of full scale @16:45:43.62 PinPort.seek :cartridge @16:45:48.18 Lysis1.seek 1350ul @16:45:50.65 Lysis2.seek 1700ul @16:45:53.75 Plunge.seek :reagent @16:45:54.59 Evacuating puck with filter bubble point of 50psi @16:45:54.62 PinPort.seek :cartridge @16:45:54.70 Handoff.select :waste @16:46:02.00 Air pumping 1 strokes to reach 8psi @16:46:02.02 Air.reconfigure :gulp @16:46:02.09 Air.seek :tVent @16:46:03.88 -0.0psi after gulp @16:46:03.89 Air stroke #1 of 1 @16:46:03.91 Air.reconfigure :pressurize @16:46:03.98 Air.seek :pressurized @16:46:16.83 0.0psi after pressurize @16:46:16.84 Air.reconfigure :compress @16:46:16.91 Air.seek :evac @16:46:19.58 0.2psi after compress @16:46:33.87 0.4psi after compress (stable) @16:46:34.00 at 0.4psi Opened to waste @16:46:34.01 Handoff.select :blocked @16:46:34.19 Air pumping 10 strokes to reach 50psi @16:46:34.20 Air.reconfigure :gulp @16:46:34.28 Air.seek :tVent @16:46:44.18 3.3psi after gulp @16:46:44.19 Air stroke #1 of 10 @16:46:44.21 Air.reconfigure :pressurize @16:46:44.35 Air.seek :pressurized @16:46:57.17 5.3psi after pressurize @16:46:57.18 Air.reconfigure :compress @16:46:57.26 Air.seek :evac @16:46:59.94 5.6psi after compress @16:47:45.90 25.2psi after compress (stable) @16:47:45.92 Air.reconfigure :gulp @16:47:45.99 Air.seek :tVent @16:47:55.88 27.0psi after gulp @16:47:55.89 Air stroke #2 of 10 @16:47:55.90 Air.reconfigure :pressurize @16:47:55.99 Air.seek :pressurized @16:48:08.81 26.9psi after pressurize @16:48:08.82 Air.reconfigure :compress @16:48:08.90 Air.seek :evac @16:48:11.57 26.0psi after compress @16:48:57.34 39.6psi after compress (stable) @16:48:57.36 Air.reconfigure :gulp @16:48:57.43 Air.seek :tVent @16:49:07.32 42.1psi after gulp @16:49:07.34 Air stroke #3 of 10 @16:49:07.36 Air.reconfigure :pressurize @16:49:07.44 Air.seek :pressurized @16:49:20.26 41.3psi after pressurize @16:49:20.27 Air.reconfigure :compress @16:49:20.35 Air.seek :evac @16:49:23.01 39.0psi after compress @16:50:04.38 Reached 50.0 of 50.0psi after pumping 6230ul (4.00 strokes) @16:50:04.39 Handoff.select :waste @16:50:13.95 delay 30 seconds @16:50:43.97 Handoff.select :blocked @16:51:01.33 Pressure too High, open to waste again (8.2psi) @16:51:01.34 Handoff.select :waste @16:51:01.46 delay 30 seconds @16:51:31.48 Handoff.select :blocked @16:51:38.90 Pressure too High, open to waste again (8.0psi) @16:51:38.91 Handoff.select :waste @16:51:39.04 delay 30 seconds @16:52:09.06 Handoff.select :blocked @16:52:35.57 Handoff.select :waste @16:52:35.71 PSI at @3.55psi at evacEnd @16:52:35.72 Handoff.select :blocked @16:52:35.84 Air.reconfigure :gulp @16:52:35.95 Air.seek :tVent @16:52:53.02 Stable @3.0psi after Handoff blocked @16:52:53.10 Air pumping 10 strokes to reach 33psi @16:52:53.11 Air stroke #1 of 10 @16:52:53.14 Air.reconfigure :pressurize @16:52:53.22 Air.seek :pressurized @16:53:06.05 3.5psi after pressurize @16:53:06.06 Air.reconfigure :compress @16:53:06.14 Air.seek :evac @16:53:08.81 3.9psi after compress @16:53:34.37 11.4psi after compress (stable) @16:53:34.39 Air.reconfigure :gulp @16:53:34.46 Air.seek :tVent @16:53:44.35 11.9psi after gulp @16:53:44.36 Air stroke #2 of 10 @16:53:44.38 Air.reconfigure :pressurize @16:53:44.46 Air.seek :pressurized @16:53:57.29 12.1psi after pressurize @16:53:57.30 Air.reconfigure :compress @16:53:57.38 Air.seek :evac @16:54:00.05 12.3psi after compress @16:54:24.85 19.2psi after compress (stable) @16:54:24.87 Air.reconfigure :gulp @16:54:24.95 Air.seek :tVent @16:54:34.85 19.7psi after gulp @16:54:34.86 Air stroke #3 of 10 @16:54:34.88 Air.reconfigure :pressurize @16:54:34.96 Air.seek :pressurized @16:54:47.79 19.9psi after pressurize @16:54:47.80 Air.reconfigure :compress @16:54:47.87 Air.seek :evac @16:54:50.54 19.9psi after compress @16:55:13.31 25.8psi after compress (stable) @16:55:13.33 Air.reconfigure :gulp @16:55:13.40 Air.seek :tVent @16:55:23.28 26.6psi after gulp @16:55:23.35 Air stroke #4 of 10 @16:55:23.37 Air.reconfigure :pressurize @16:55:23.44 Air.seek :pressurized @16:55:36.29 26.6psi after pressurize @16:55:36.37 Air.reconfigure :compress @16:55:36.44 Air.seek :evac @16:55:39.12 26.7psi after compress @16:56:03.37 32.0psi after compress (stable) @16:56:03.39 Air.reconfigure :gulp @16:56:03.47 Air.seek :tVent @16:56:13.35 32.8psi after gulp @16:56:13.42 Air stroke #5 of 10 @16:56:13.43 Air.reconfigure :pressurize @16:56:13.52 Air.seek :pressurized @16:56:26.34 32.9psi after pressurize @16:56:26.43 Air.reconfigure :compress @16:56:26.51 Air.seek :evac @16:56:26.70 Reached 33.0 of 33.0psi after pumping 7625ul (4.90 strokes) @16:56:26.71 Handoff.select :waste @16:56:34.66 delay 30 seconds @16:57:04.68 Handoff.select :blocked @16:57:30.76 Handoff.select :waste @16:57:30.87 PSI at @3.66psi at evacEnd @16:57:30.88 Air.reconfigure :default @16:57:31.00 Air.seek :fVent @16:57:33.86 delay 5 seconds @16:57:38.88 Seawater evacuated, Presure above/below filter released. Lysis begins @16:57:38.95 Lysis1.reconfigure :slow @16:57:39.02 Lysis1.seek 1050ul @16:58:09.02 delay 5 seconds @16:58:14.04 Handoff.select :blocked @16:58:14.21 Lysis1.seek 450ul @16:59:14.16 delay 3 seconds @16:59:17.23 Air.reconfigure to mix into 1.37806 parts Lysis1 @16:59:17.78 Air.seek :evac @16:59:17.81 Lysis1.seek 300ul @16:59:32.92 Air.reconfigure :default @16:59:32.98 Air.seek :full @16:59:47.20 Lysis1.seek :empty @17:00:17.28 Lysis Delivered. Syringe pressure @14.9psi @17:00:17.32 PuckHeater.seek 84.5C @17:01:06.82 PuckHeater.hold 15:00,84.5C @17:16:05.22 Lysis complete. Syringe pressure @19.4psi @17:16:05.23 Handoff.select :blocked @17:16:05.33 Lysis1.reconfigure :halfSpeed @17:16:05.40 Lysis1.seek 200ul @17:16:09.17 delay 1 second @17:16:10.19 Lysis1.seek :empty @17:16:14.01 Initial AirS pressure @20.0psi @17:16:14.07 Lysis1.reconfigure :extract @17:16:14.16 Lysis1.seek 900ul,10:00 @17:16:15.34 Air pumping 10 strokes to reach 21psi @17:16:15.35 Air stroke #1 of 10 @17:16:15.37 Air.reconfigure :pressurize @17:16:15.45 Air.seek :pressurized @17:16:28.28 10.3psi after pressurize @17:16:28.29 Air.reconfigure :compress @17:16:28.36 Air.seek :evac @17:16:31.03 10.3psi after compress @17:16:45.30 10.4psi after compress (stable) @17:16:45.32 Air.reconfigure :gulp @17:16:45.40 Air.seek :tVent @17:16:55.28 10.6psi after gulp @17:16:55.29 Air stroke #2 of 10 @17:16:55.30 Air.reconfigure :pressurize @17:16:55.39 Air.seek :pressurized @17:17:08.21 10.6psi after pressurize @17:17:08.24 Air.reconfigure :compress @17:17:08.32 Air.seek :evac @17:17:10.98 10.7psi after compress @17:17:24.87 10.7psi after compress (stable) @17:17:24.89 Air.reconfigure :gulp @17:17:24.96 Air.seek :tVent @17:17:34.85 10.7psi after gulp @17:17:34.86 Air stroke #3 of 10 @17:17:34.87 Air.reconfigure :pressurize @17:17:34.96 Air.seek :pressurized @17:17:47.78 10.7psi after pressurize @17:17:47.79 Air.reconfigure :compress @17:17:47.87 Air.seek :evac @17:17:50.53 10.8psi after compress @17:18:04.42 10.7psi after compress (stable) @17:18:04.44 Air.reconfigure :gulp @17:18:04.51 Air.seek :tVent @17:18:14.40 10.7psi after gulp @17:18:14.41 Air stroke #4 of 10 @17:18:14.43 Air.reconfigure :pressurize @17:18:14.53 Air.seek :pressurized @17:18:27.35 10.7psi after pressurize @17:18:27.36 Air.reconfigure :compress @17:18:27.44 Air.seek :evac @17:18:30.11 10.8psi after compress @17:18:43.99 10.7psi after compress (stable) @17:18:44.01 Air.reconfigure :gulp @17:18:44.08 Air.seek :tVent @17:18:54.35 10.7psi after gulp @17:18:54.36 Air stroke #5 of 10 @17:18:54.38 Air.reconfigure :pressurize @17:18:54.46 Air.seek :pressurized @17:19:07.27 10.7psi after pressurize @17:19:07.28 Air.reconfigure :compress @17:19:07.36 Air.seek :evac @17:19:10.03 10.8psi after compress @17:19:23.94 10.7psi after compress (stable) @17:19:23.95 Air.reconfigure :gulp @17:19:24.03 Air.seek :tVent @17:19:33.91 10.8psi after gulp @17:19:33.93 Air stroke #6 of 10 @17:19:33.96 Air.reconfigure :pressurize @17:19:34.04 Air.seek :pressurized @17:19:46.86 10.7psi after pressurize @17:19:46.87 Air.reconfigure :compress @17:19:46.95 Air.seek :evac @17:19:49.61 10.8psi after compress @17:20:03.54 10.7psi after compress (stable) @17:20:03.56 Air.reconfigure :gulp @17:20:03.63 Air.seek :tVent @17:20:13.51 10.7psi after gulp @17:20:13.52 Air stroke #7 of 10 @17:20:13.54 Air.reconfigure :pressurize @17:20:13.62 Air.seek :pressurized @17:20:26.45 10.7psi after pressurize @17:20:26.46 Air.reconfigure :compress @17:20:26.54 Air.seek :evac @17:20:29.21 10.8psi after compress @17:20:43.10 10.7psi after compress (stable) @17:20:43.12 Air.reconfigure :gulp @17:20:43.19 Air.seek :tVent @17:20:53.07 10.7psi after gulp @17:20:53.08 Air stroke #8 of 10 @17:20:53.09 Air.reconfigure :pressurize @17:20:53.17 Air.seek :pressurized @17:21:06.00 10.7psi after pressurize @17:21:06.01 Air.reconfigure :compress @17:21:06.09 Air.seek :evac @17:21:08.75 10.8psi after compress @17:21:23.07 10.6psi after compress (stable) @17:21:23.08 Air.reconfigure :gulp @17:21:23.16 Air.seek :tVent @17:21:33.04 10.7psi after gulp @17:21:33.05 Air stroke #9 of 10 @17:21:33.08 Air.reconfigure :pressurize @17:21:33.17 Air.seek :pressurized @17:21:45.98 10.6psi after pressurize @17:21:45.99 Air.reconfigure :compress @17:21:46.07 Air.seek :evac @17:21:48.73 10.7psi after compress @17:22:02.72 10.6psi after compress (stable) @17:22:02.73 Air.reconfigure :gulp @17:22:02.81 Air.seek :tVent @17:22:12.68 10.7psi after gulp @17:22:12.69 Air stroke #10 of 10 @17:22:12.71 Air.reconfigure :pressurize @17:22:12.79 Air.seek :pressurized @17:22:25.63 10.6psi after pressurize @17:22:25.64 Air.reconfigure :compress @17:22:25.72 Air.seek :evac @17:22:28.39 10.7psi after compress @17:22:42.34 10.6psi after compress (stable) @17:22:42.42 Syringe::Underpressure in pump -- Reached only 10.6 of 21.0psi after pumping 15575ul @17:22:42.45 Lysis1.reconfigure :default @17:22:42.51 Lysis1.hold @17:22:42.67 Thread::ChildDied in PROCESSING -- :pump during Lysis Syringe #1 (LyS1) move -- Skipping SPR @17:22:42.68 SPR.command \"start 2 14\" @17:22:43.54 Handoff.select :waste @17:22:44.03 Ended log /var/log/esp/spr19aug2716h.spr @17:22:44.04 SPR.command \"stats_off\" @17:22:46.54 -> Cmd.stop @17:22:46.63 Handoff.select :close @17:22:46.75 Gate.power :heat,:OFF @17:22:46.77 Gate.power :cartridge,:OFF @17:22:46.85 Carousel.seek 3 @17:22:47.02 Plunge.seek :locked @17:22:47.86 PinPort.seek :clear @17:22:52.47 Archive.seek :clear @17:22:52.60 Air.reconfigure :default @17:22:52.73 Lysis1.seek :clear @17:22:52.82 Air.seek :clear @17:22:52.91 Lysis2.seek :clear @17:23:10.20 Archive.coast @17:23:10.25 Air.coast @17:23:10.30 Lysis1.coast @17:23:10.35 Lysis2.coast @17:23:10.40 Plunge.coast @17:23:10.46 SamplePump.coast @17:23:10.51 Toroid.coast @17:23:10.56 Carousel.coast @17:23:10.61 PinPort.coast @17:23:11.16 Gate.power :motor,:OFF @17:23:11.19 Gate.power :core,:OFF @17:23:11.21 Gate.power :analytic1,:OFF @17:23:11.23 Gate.power :analytic2,:OFF @17:23:11.25 Gate.power :analytic3,:OFF @17:23:11.28 Gate.power :main,:OFF @17:23:11.30 Safely stopped and ready to power off