##-- file opened 2018-08-27T12:42:56.299Z @08:42:33.45 -> showStatus @08:42:35.09 -> Cmd.loadCartridge @08:42:35.21 Gate.power :main,:ON,limit to 5000mA @08:42:35.23 Gate.power :analytic1,:ON,limit to 5000mA @08:42:35.25 Gate.power :analytic2,:ON,limit to 5000mA @08:42:35.27 Gate.power :analytic3,:ON,limit to 5000mA @08:42:35.42 Powering core bus @08:42:37.72 SPR Module 1, FW Rev. B, FPGA Rev. C @08:42:37.82 ArS.configure ArSconfig @08:42:37.92 LyS1.configure LyS1config @08:42:38.04 LyS2.configure LyS2config @08:42:38.15 AirS.configure AirSconfig @08:42:38.26 TV.configure TVconfig @08:42:38.38 SP.configure SPconfig @08:42:38.51 SC.configure SCconfig @08:42:38.63 PV.configure PVconfig @08:42:38.74 PinPort.configure PPconfig @08:42:38.86 Sea.configure SPconfig @08:42:38.92 All elf controllers running firmware version 4.5 @08:42:38.94 Gate.power :motor,:ON,limit to 5000mA @08:42:39.13 ArS.reconfigure ArSconfig @08:42:39.38 ArS.home.jog 0 @08:42:39.53 PinPort.reconfigure PPconfig @08:42:39.58 PV.reconfigure PVconfig @08:42:39.62 AirS.reconfigure AirSconfig @08:42:39.65 LyS1.reconfigure LyS1config @08:42:39.69 TV.reconfigure TVconfig @08:42:39.75 LyS2.reconfigure LyS2config @08:42:39.98 PinPort.home.jog 0 @08:42:40.02 PV.home.jog 0 @08:42:40.07 AirS.home.jog 0 @08:42:40.09 LyS1.home.jog 0 @08:42:40.13 TV.home.jog 0 @08:42:40.17 LyS2.home.jog 0 @08:42:40.55 ArS.reconfigure HomeMagSyringe @08:42:40.76 ArS.jog -253 @08:42:40.92 TV.reconfigure HomeTV @08:42:40.95 AirS.reconfigure HomeMagSyringe @08:42:40.98 LyS2.reconfigure HomeSyringe @08:42:41.02 PinPort.reconfigure HomePP @08:42:41.06 PV.reconfigure HomePV @08:42:41.09 LyS1.reconfigure HomeSyringe @08:42:41.25 AirS.jog -319 @08:42:41.32 LyS2.jog -267 @08:42:41.34 TV.jog -544 @08:42:41.37 PinPort.jog -110 @08:42:41.43 PV.jog -1474 @08:42:41.46 LyS1.jog -260 @08:42:41.69 ArS.setRawPosition! -50 @08:42:41.74 ArS.seek :home @08:42:41.88 PinPort.setRawPosition! 0 @08:42:41.93 PinPort.seek :home @08:42:42.30 AirS.setRawPosition! -50 @08:42:42.34 LyS2.setRawPosition! -50 @08:42:42.36 LyS1.setRawPosition! -50 @08:42:42.39 PinPort.reconfigure PPconfig @08:42:42.42 ArS.reconfigure ArSconfig @08:42:42.59 LyS2.seek :home @08:42:42.63 AirS.seek :home @08:42:42.66 LyS1.seek :home @08:42:42.84 PV.setRawPosition! -50 @08:42:42.89 PV.seek :home @08:42:42.98 LyS2.reconfigure LyS2config @08:42:43.10 AirS.reconfigure AirSconfig @08:42:43.12 LyS1.reconfigure LyS1config @08:42:43.26 PV.reconfigure PVconfig @08:42:43.36 TV.setRawPosition! 0 @08:42:43.41 TV.seek :home @08:42:43.52 TV.reconfigure TVconfig @08:42:43.69 TV.seek :clear @08:42:45.31 TV.coast @08:42:45.36 SC.reconfigure HomeSC @08:42:45.42 SC.home.jog 0 @08:42:46.19 SC.reconfigure SCconfig @08:42:46.25 SC.seek 39 @08:42:46.35 Selecting Cartridge 38 @08:42:46.66 SC.seek 38 @08:42:47.15 LyS2.seek :locked @08:42:47.28 PV.seek :locked @08:42:47.41 AirS.seek :locked @08:42:51.48 Powering cartridge bus @08:42:53.60 Gate.setIR 1750,5000,0,6 @08:42:53.73 Gate.setIR 0,5000,0,6 @08:42:53.77 Loaded Archive Cartridge Gate.power :cartridge,:OFF @08:43:13.41 -> Cmd.startFiltering @08:43:13.64 Duration of filtering limited to 1:05:00 @08:43:13.89 Priming sample loop w/100ml, bypass w/10ml @08:43:13.97 SP.setPosition! 0ml @08:43:14.01 Intake.open @08:43:14.03 Exhaust.open @08:43:14.88 Sea @-42.9psia @08:43:14.90 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @08:43:20.68 Vacuum tare is 24% of full scale @08:43:20.69 PB.open @08:43:20.72 Exhaust.close @08:43:21.53 Sea @-42.9psia @08:43:21.61 SP.reconfigure SPprime @08:43:21.68 SP.seek 10ml,1:21 @08:43:32.53 SP.setPosition! 0ml @08:43:32.58 PB.close @08:43:32.60 Exhaust.open @08:43:33.43 Sea @-42.9psia @08:43:33.52 SP.reconfigure SPprime @08:43:33.58 SP.seek 100ml,8:51 @08:45:28.13 Intake.close @08:45:28.90 Pressurizing cartridge to 26psi (try #1 of 3) @08:45:28.91 SP.setPosition! 0ml @08:45:28.97 PB.open @08:45:29.76 TV.seek :crack @08:45:30.55 TV.seek :clear @08:45:31.35 Intake.open @08:45:32.81 PB.close @08:45:33.54 Exhaust.close @08:45:34.32 Sea @-42.9psia @08:45:34.40 SP.reconfigure SPcharge @08:45:34.47 SP.seek 2.3ml,42 seconds @08:45:41.79 Cartridge pressurized with 0.249ml to 27.2psi @08:45:42.02 delay 3 seconds @08:45:45.09 Cartridge at 22.6psi 3 seconds later @08:45:45.17 TV.seek :cartridge @08:45:46.99 TV.seek :clear @08:45:48.81 Sampling 1000.0ml @08:45:48.82 SP.setPosition! 0ml @08:45:48.87 Exhaust.open @08:45:49.62 Sea @-42.9psia @08:45:49.71 SP.reconfigure SPsample @08:45:49.78 SP.seek 1000ml,1:23:53 @08:45:50.73 Cartridge 38 sampling 0/1000ml at 0.17ml/s with 6.5psi vacuum @08:46:50.74 Cartridge 38 sampling 22/1000ml at 0.36ml/s with 22.2psi vacuum @08:47:50.73 Cartridge 38 sampling 39/1000ml at 0.27ml/s with 24.5psi vacuum @08:48:50.74 Cartridge 38 sampling 53/1000ml at 0.20ml/s with 26.2psi vacuum @08:49:41.54 Flow averaged 0.28ml/s over 3:52 @08:49:41.56 Waiting up to 23 seconds for vacuum to reduce from 32.1 to 18.0psi @08:49:42.18 Relieved excess vacuum in 1.3 seconds @08:49:42.20 SP.seek 1000ml,1:18:35 @08:49:49.43 Flow estimate reduced to 0.22ml/s @08:49:49.45 Waiting up to 16 seconds for vacuum to reduce from 29.3 to 18.0psi @08:49:50.07 Relieved excess vacuum in 4.8 seconds @08:49:50.10 SP.seek 1000ml,1:18:28 @08:49:50.73 Cartridge 38 sampling 65/1000ml at 0.10ml/s with 7.9psi vacuum @08:49:53.91 Flow estimate reduced to 0.18ml/s @08:49:53.93 Intake.close @08:49:53.96 Exhaust.close @08:49:54.77 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @08:49:54.87 Sampled 65.6ml @08:49:54.95 TV.seek :bypass @08:49:56.56 TV.seek :clear @08:49:58.84 -> Cmd.startProcessing @08:49:59.14 PV.seek :reagent @08:50:00.25 Sample loop closed. Ready to Deliver Reagents @08:50:00.26 ArS.seek :sealed @08:50:04.53 ArS.seek :evac @08:50:18.48 delay 20 seconds @08:50:38.52 ArS.seek :clear @08:50:56.23 RNAlater soak for 10:00 @09:00:56.27 ArS.seek :tVent @09:00:58.84 delay 1 second @09:00:59.87 ArS.seek :evac @09:01:15.49 delay 10 seconds @09:01:25.52 Sample preserved and evacuated @09:01:26.53 -> Cmd.stop @09:01:26.67 PV.seek :locked @09:01:27.57 PinPort.seek :clear @09:01:27.71 ArS.seek :clear @09:01:27.89 LyS2.seek :clear @09:01:28.02 AirS.seek :clear @09:01:45.46 ArS.coast @09:01:45.51 LyS1.coast @09:01:45.56 LyS2.coast @09:01:45.61 AirS.coast @09:01:45.66 TV.coast @09:01:45.70 SP.coast @09:01:45.75 SC.coast @09:01:45.80 PV.coast @09:01:45.86 PinPort.coast @09:01:46.03 Gate.power :motor,:OFF @09:01:46.05 Gate.power :main,:OFF @09:01:46.07 Gate.power :core,:OFF @09:01:46.09 Gate.power :analytic1,:OFF @09:01:46.11 Gate.power :analytic2,:OFF @09:01:46.13 Gate.power :analytic3,:OFF @09:01:46.15 Safely stopped and ready to power off ##-- file closed 2018-08-27T13:14:06.857Z ##-- file opened 2018-08-27T13:14:06.859Z @09:13:40.78 -> showStatus @09:13:42.79 -> Cmd.loadCartridge @09:13:42.91 Gate.power :main,:ON,limit to 5000mA @09:13:42.92 Gate.power :analytic1,:ON,limit to 5000mA @09:13:42.94 Gate.power :analytic2,:ON,limit to 5000mA @09:13:42.97 Gate.power :analytic3,:ON,limit to 5000mA @09:13:43.11 Powering core bus @09:13:45.42 SPR Module 1, FW Rev. B, FPGA Rev. C @09:13:45.52 ArS.configure ArSconfig @09:13:45.62 LyS1.configure LyS1config @09:13:45.74 LyS2.configure LyS2config @09:13:45.84 AirS.configure AirSconfig @09:13:45.96 TV.configure TVconfig @09:13:46.08 SP.configure SPconfig @09:13:46.20 SC.configure SCconfig @09:13:46.32 PV.configure PVconfig @09:13:46.44 PinPort.configure PPconfig @09:13:46.56 Sea.configure SPconfig @09:13:46.62 All elf controllers running firmware version 4.5 @09:13:46.64 Gate.power :motor,:ON,limit to 5000mA @09:13:46.81 ArS.reconfigure ArSconfig @09:13:47.05 ArS.home.jog 0 @09:13:47.18 PV.reconfigure PVconfig @09:13:47.25 TV.reconfigure TVconfig @09:13:47.28 AirS.reconfigure AirSconfig @09:13:47.32 LyS1.reconfigure LyS1config @09:13:47.35 PinPort.reconfigure PPconfig @09:13:47.41 LyS2.reconfigure LyS2config @09:13:47.62 TV.home.jog 0 @09:13:47.65 PV.home.jog 0 @09:13:47.67 AirS.home.jog 0 @09:13:47.70 LyS1.home.jog 0 @09:13:47.72 PinPort.home.jog 0 @09:13:47.75 LyS2.home.jog 0 @09:13:48.21 ArS.reconfigure HomeMagSyringe @09:13:48.39 PV.reconfigure HomePV @09:13:48.43 TV.reconfigure HomeTV @09:13:48.50 ArS.jog -239 @09:13:48.56 PinPort.reconfigure HomePP @09:13:48.59 AirS.reconfigure HomeMagSyringe @09:13:48.74 PV.jog -1480 @09:13:48.78 TV.jog -544 @09:13:48.81 PinPort.jog -110 @09:13:48.85 AirS.jog -320 @09:13:49.04 LyS1.reconfigure HomeSyringe @09:13:49.06 LyS2.reconfigure HomeSyringe @09:13:49.16 LyS2.jog -264 @09:13:49.19 LyS1.jog -266 @09:13:49.33 PinPort.setRawPosition! 0 @09:13:49.35 ArS.setRawPosition! -50 @09:13:49.41 PinPort.seek :home @09:13:49.46 ArS.seek :home @09:13:49.89 PinPort.reconfigure PPconfig @09:13:49.90 AirS.setRawPosition! -50 @09:13:49.92 LyS2.setRawPosition! -50 @09:13:49.96 LyS1.setRawPosition! -50 @09:13:50.08 AirS.seek :home @09:13:50.18 LyS2.seek :home @09:13:50.23 LyS1.seek :home @09:13:50.38 PV.setRawPosition! -50 @09:13:50.40 ArS.reconfigure ArSconfig @09:13:50.48 PV.seek :home @09:13:50.63 AirS.reconfigure AirSconfig @09:13:50.66 LyS2.reconfigure LyS2config @09:13:50.69 LyS1.reconfigure LyS1config @09:13:50.91 TV.setRawPosition! 0 @09:13:50.93 PV.reconfigure PVconfig @09:13:51.00 TV.seek :home @09:13:51.12 TV.reconfigure TVconfig @09:13:51.34 TV.seek :clear @09:13:52.96 TV.coast @09:13:53.01 SC.reconfigure HomeSC @09:13:53.07 SC.home.jog 0 @09:13:53.83 SC.reconfigure SCconfig @09:13:53.89 SC.seek 38 @09:13:53.99 Selecting Cartridge 37 @09:13:54.30 SC.seek 37 @09:13:54.80 LyS2.seek :locked @09:13:54.93 PV.seek :locked @09:13:55.06 AirS.seek :locked @09:13:59.14 Powering cartridge bus @09:14:01.27 Gate.setIR 1750,5000,0,6 @09:14:01.39 Gate.setIR 0,5000,0,6 @09:14:01.44 Loaded Lyse-n-Go Cartridge @09:14:01.45 PuckHeater.configure HeatConfig @09:14:01.51 Gate.power :heat,:ON,limit to 5000mA @09:14:21.45 -> Cmd.startFiltering @09:14:21.68 Duration of filtering limited to 1:05:00 @09:14:21.93 Priming sample loop w/100ml, bypass w/10ml @09:14:22.01 SP.setPosition! 0ml @09:14:22.05 Intake.open @09:14:22.11 Exhaust.open @09:14:22.87 Sea @-42.9psia @09:14:22.89 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @09:14:28.67 Vacuum tare is 24% of full scale @09:14:28.68 PB.open @09:14:28.73 Exhaust.close @09:14:29.52 Sea @-42.9psia @09:14:29.60 SP.reconfigure SPprime @09:14:29.67 SP.seek 10ml,1:21 @09:14:40.52 SP.setPosition! 0ml @09:14:40.56 PB.close @09:14:40.59 Exhaust.open @09:14:41.43 Sea @-42.9psia @09:14:41.52 SP.reconfigure SPprime @09:14:41.58 SP.seek 100ml,8:51 @09:16:35.83 Intake.close @09:16:36.56 Pressurizing cartridge to 26psi (try #1 of 3) @09:16:36.57 SP.setPosition! 0ml @09:16:36.62 PB.open @09:16:37.38 TV.seek :crack @09:16:38.17 TV.seek :clear @09:16:38.97 Intake.open @09:16:40.42 PB.close @09:16:41.14 Exhaust.close @09:16:41.90 Sea @-42.9psia @09:16:41.98 SP.reconfigure SPcharge @09:16:42.05 SP.seek 2.3ml,42 seconds @09:16:50.66 Cartridge pressurized with 0.345ml to 26.4psi @09:16:51.04 delay 3 seconds @09:16:54.11 Cartridge at 21.3psi 3 seconds later @09:16:54.19 TV.seek :cartridge @09:16:56.00 TV.seek :clear @09:16:57.81 Sampling 1000.0ml @09:16:57.82 SP.setPosition! 0ml @09:16:57.87 Exhaust.open @09:16:58.68 Sea @-42.9psia @09:16:58.77 SP.reconfigure SPsample @09:16:58.84 SP.seek 1000ml,1:23:53 @09:16:59.79 Cartridge 37 sampling 0/1000ml at 0.17ml/s with 4.2psi vacuum @09:17:59.80 Cartridge 37 sampling 32/1000ml at 0.54ml/s with 26.7psi vacuum @09:18:59.79 Cartridge 37 sampling 64/1000ml at 0.51ml/s with 24.6psi vacuum @09:19:59.80 Cartridge 37 sampling 95/1000ml at 0.50ml/s with 20.0psi vacuum @09:20:59.80 Cartridge 37 sampling 125/1000ml at 0.50ml/s with 22.2psi vacuum @09:21:59.80 Cartridge 37 sampling 153/1000ml at 0.45ml/s with 21.6psi vacuum @09:22:59.80 Cartridge 37 sampling 178/1000ml at 0.38ml/s with 20.0psi vacuum @09:23:59.79 Cartridge 37 sampling 200/1000ml at 0.31ml/s with 19.0psi vacuum @09:24:59.80 Cartridge 37 sampling 217/1000ml at 0.26ml/s with 23.2psi vacuum @09:25:59.80 Cartridge 37 sampling 232/1000ml at 0.27ml/s with 22.1psi vacuum @09:26:59.80 Cartridge 37 sampling 245/1000ml at 0.20ml/s with 22.8psi vacuum @09:27:59.80 Cartridge 37 sampling 257/1000ml at 0.20ml/s with 28.6psi vacuum @09:28:17.21 Flow averaged 0.39ml/s over 11:12 @09:28:17.23 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi @09:28:17.86 Relieved excess vacuum in 8 seconds @09:28:17.88 SP.seek 1000ml,1:02:08 @09:28:37.58 Flow averaged 0.20ml/s over 19 seconds @09:28:37.60 Waiting up to 17 seconds for vacuum to reduce from 29.6 to 18.0psi @09:28:38.23 Relieved excess vacuum in 1.9 seconds @09:28:38.26 SP.seek 1000ml,1:01:49 @09:28:43.90 Flow estimate reduced to 0.16ml/s @09:28:43.91 Intake.close @09:28:43.95 Exhaust.close @09:28:44.76 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @09:28:44.86 Sampled 265.6ml @09:28:44.94 TV.seek :bypass @09:28:46.56 TV.seek :clear @09:28:48.95 -> Cmd.startProcessing @09:28:50.75 SPR.command \"read_sequences\" @09:28:51.43 SPR.command \"stats_on\" @09:28:51.47 SPR.command \"start 0 1\" @09:28:53.47 Begun log /var/log/esp/spr18aug2709h.spr @09:28:54.29 Handoff.select :waste @09:28:54.42 ArS.seek empty @09:29:12.13 PinPort.seek :cartridge @09:29:17.85 LyS1.seek 1350ul @09:29:23.79 PV.seek :reagent @09:29:24.62 AirS.seek :tVent @09:29:26.69 delay 2 seconds @09:29:28.72 AirS.reconfigure EvacAirS @09:29:28.77 AirS.seek :evac @09:29:51.10 delay 15 seconds @09:30:06.13 AirS.reconfigure AirSconfig @09:30:06.18 AirS.seek :tVent @09:30:20.40 delay 2 seconds @09:30:22.43 AirS.reconfigure EvacAirS @09:30:22.48 AirS.seek :evac @09:30:44.79 delay 15 seconds @09:30:59.82 AirS.reconfigure AirSconfig @09:30:59.87 AirS.seek :tVent @09:31:14.09 delay 2 seconds @09:31:16.12 AirS.reconfigure EvacAirS @09:31:16.17 AirS.seek :evac @09:31:38.47 delay 15 seconds @09:31:53.51 AirS.reconfigure AirSconfig @09:31:53.57 AirS.seek :tVent @09:32:07.79 delay 2 seconds @09:32:09.82 AirS.reconfigure EvacAirS @09:32:09.87 AirS.seek :evac @09:32:32.17 delay 15 seconds @09:32:47.20 AirS.reconfigure AirSconfig @09:32:47.25 AirS.seek :tVent @09:33:01.46 delay 2 seconds @09:33:03.50 AirS.reconfigure EvacAirS @09:33:03.58 AirS.seek :evac @09:33:25.88 delay 15 seconds @09:33:40.90 delay 1:30 @09:35:10.93 AirS.reconfigure AirSconfig @09:35:10.98 AirS.seek :fVent @09:35:12.42 delay 15 seconds @09:35:27.44 LyS1.reconfigure SlowLyS1 @09:35:27.54 LyS1.seek 1050ul @09:35:57.57 delay 5 seconds @09:36:02.58 Handoff.select :blocked @09:36:02.73 LyS1.seek 450ul @09:37:02.68 delay 10 seconds @09:37:12.70 AirS.reconfigure SlowAirS @09:37:12.81 AirS.reconfigure to mix into 1.37806 parts LyS1 @09:37:13.20 AirS.seek evac @09:37:13.28 LyS1.seek 300ul @09:37:28.35 AirS.reconfigure AirSconfig @09:37:28.41 AirS.seek :full @09:37:42.62 LyS1.seek :empty @09:38:03.44 Slide::Error in PROCESSING -- IGNORED Lysis Syringe #1 positionErr at 93ul (actually 149ul) @09:38:03.46 LyS1.reconfigure SlowLyS1 @09:38:03.53 LyS1.home.jog 0 @09:38:03.67 PuckHeater.seek 84.3C @09:39:02.67 PuckHeater.hold 15:00,84.3C @09:54:00.73 LyS1.reconfigure LyS1config @09:54:00.79 LyS1.seek 900ul @09:54:07.82 AirS.seek :tVent @09:54:07.91 delay 2 seconds @09:54:09.93 AirS.reconfigure HalfSpeedAirS @09:54:10.00 AirS.seek :evac @09:54:37.75 delay 15 seconds @09:54:52.77 AirS.reconfigure AirSconfig @09:54:52.87 AirS.seek :tVent @09:55:07.07 delay 2 seconds @09:55:09.10 AirS.reconfigure HalfSpeedAirS @09:55:09.15 AirS.seek :evac @09:55:36.89 delay 15 seconds @09:55:51.92 AirS.reconfigure AirSconfig @09:55:51.97 AirS.seek :tVent @09:56:06.18 delay 2 seconds @09:56:08.21 AirS.reconfigure HalfSpeedAirS @09:56:08.27 AirS.seek :evac @09:56:36.02 delay 15 seconds @09:56:51.04 delay 1:00 @09:57:51.06 Handoff.select :waste @09:57:51.16 delay 15 seconds @09:58:06.19 AirS.seek :fVent @09:58:08.38 delay 10 seconds @09:58:18.41 AirS.reconfigure AirSconfig @09:58:18.46 AirS.seek :evac @09:58:19.90 Lysis Recovered in LyS1 @09:58:19.91 Lysis Complete @09:58:19.92 Handoff.select :waste @09:58:19.95 LyS2.seek :full @09:58:20.03 LyS1.reconfigure DAmixLyS1 @09:58:20.09 LyS2.reconfigure DAmixLyS2 @09:58:20.49 LyS2.seek 1480ul @09:58:20.52 LyS1.seek 650ul @09:59:07.87 delay 5 seconds @09:59:12.89 Handoff.select :SPR @09:59:13.00 SPR.command \"start 2 14\" @09:59:14.06 Running Experiment 2 @09:59:14.07 delay 10 seconds @09:59:24.09 SPR.command \"start 2 0\" @09:59:27.42 Waiting up to 11:00 for SPR to enter state 1 @10:06:45.71 SPR.command \"hold_on\" @10:06:47.41 LyS2.seek 1130ul @10:06:47.44 LyS1.seek 300ul @10:07:53.64 delay 15 seconds @10:08:08.66 SPR.command \"hold_off\" @10:08:11.91 Waiting up to 25:00 for SPR to enter state 7 @10:19:48.84 Handoff.select :waste @10:19:48.96 LyS1.reconfigure LyS1config @10:19:49.03 LyS2.reconfigure LyS2config @10:19:49.09 Rep #1 SPR Analysis has Completed @10:19:49.78 Ended log /var/log/esp/spr18aug2709h.spr @10:19:49.79 SPR.command \"stats_off\" @10:19:51.90 -> Cmd.stop @10:19:51.99 Handoff.select :close @10:19:52.16 Gate.power :heat,:OFF @10:19:52.17 Gate.power :cartridge,:OFF @10:19:52.20 PV.seek :locked @10:19:53.10 PinPort.seek :clear @10:19:59.10 ArS.seek :clear @10:19:59.22 LyS1.seek :clear @10:19:59.36 LyS2.seek :clear @10:19:59.49 AirS.seek :clear @10:20:16.86 ArS.coast @10:20:16.90 LyS1.coast @10:20:16.95 LyS2.coast @10:20:17.00 AirS.coast @10:20:17.05 TV.coast @10:20:17.10 SP.coast @10:20:17.16 SC.coast @10:20:17.21 PV.coast @10:20:17.26 PinPort.coast @10:20:17.43 Gate.power :motor,:OFF @10:20:17.45 Gate.power :main,:OFF @10:20:17.47 Gate.power :core,:OFF @10:20:17.49 Gate.power :analytic1,:OFF @10:20:17.51 Gate.power :analytic2,:OFF @10:20:17.53 Gate.power :analytic3,:OFF @10:20:17.55 Safely stopped and ready to power off ##-- file closed 2018-08-27T14:32:32.245Z ##-- file opened 2018-08-27T14:32:32.247Z @10:31:58.91 -> showStatus @10:32:00.88 -> Cmd.loadCartridge @10:32:01.00 Gate.power :main,:ON,limit to 5000mA @10:32:01.02 Gate.power :analytic1,:ON,limit to 5000mA @10:32:01.04 Gate.power :analytic2,:ON,limit to 5000mA @10:32:01.06 Gate.power :analytic3,:ON,limit to 5000mA @10:32:01.20 Powering core bus @10:32:03.51 SPR Module 1, FW Rev. B, FPGA Rev. C @10:32:03.59 ArS.configure ArSconfig @10:32:03.69 LyS1.configure LyS1config @10:32:03.81 LyS2.configure LyS2config @10:32:03.91 AirS.configure AirSconfig @10:32:04.04 TV.configure TVconfig @10:32:04.15 SP.configure SPconfig @10:32:04.28 SC.configure SCconfig @10:32:04.39 PV.configure PVconfig @10:32:04.51 PinPort.configure PPconfig @10:32:04.63 Sea.configure SPconfig @10:32:04.69 All elf controllers running firmware version 4.5 @10:32:04.71 Gate.power :motor,:ON,limit to 5000mA @10:32:04.86 ArS.reconfigure ArSconfig @10:32:05.13 ArS.home.jog 0 @10:32:05.27 PV.reconfigure PVconfig @10:32:05.34 TV.reconfigure TVconfig @10:32:05.37 LyS2.reconfigure LyS2config @10:32:05.41 PinPort.reconfigure PPconfig @10:32:05.45 LyS1.reconfigure LyS1config @10:32:05.51 AirS.reconfigure AirSconfig @10:32:05.69 TV.home.jog 0 @10:32:05.73 PV.home.jog 0 @10:32:05.76 PinPort.home.jog 0 @10:32:05.78 LyS2.home.jog 0 @10:32:05.81 AirS.home.jog 0 @10:32:05.86 LyS1.home.jog 0 @10:32:06.15 ArS.reconfigure HomeMagSyringe @10:32:06.37 PV.reconfigure HomePV @10:32:06.40 TV.reconfigure HomeTV @10:32:06.49 PinPort.reconfigure HomePP @10:32:06.54 ArS.jog -247 @10:32:06.68 TV.jog -543 @10:32:06.72 PV.jog -1484 @10:32:06.75 PinPort.jog -109 @10:32:07.00 LyS2.reconfigure HomeSyringe @10:32:07.03 LyS1.reconfigure HomeSyringe @10:32:07.09 AirS.reconfigure HomeMagSyringe @10:32:07.17 LyS2.jog -266 @10:32:07.23 LyS1.jog -272 @10:32:07.30 AirS.jog -330 @10:32:07.41 PinPort.setRawPosition! 0 @10:32:07.42 ArS.setRawPosition! -50 @10:32:07.49 PinPort.seek :home @10:32:07.53 ArS.seek :home @10:32:07.88 PinPort.reconfigure PPconfig @10:32:08.03 AirS.setRawPosition! -50 @10:32:08.07 LyS2.setRawPosition! -50 @10:32:08.10 LyS1.setRawPosition! -50 @10:32:08.12 PV.setRawPosition! -50 @10:32:08.14 ArS.reconfigure ArSconfig @10:32:08.27 AirS.seek :home @10:32:08.34 LyS2.seek :home @10:32:08.38 PV.seek :home @10:32:08.43 LyS1.seek :home @10:32:08.64 TV.setRawPosition! 0 @10:32:08.69 TV.seek :home @10:32:08.86 PV.reconfigure PVconfig @10:32:08.90 TV.reconfigure TVconfig @10:32:08.93 LyS2.reconfigure LyS2config @10:32:08.98 AirS.reconfigure AirSconfig @10:32:09.00 LyS1.reconfigure LyS1config @10:32:09.66 TV.seek :clear @10:32:11.28 TV.coast @10:32:11.33 SC.reconfigure HomeSC @10:32:11.40 SC.home.jog 0 @10:32:12.14 SC.reconfigure SCconfig @10:32:12.20 SC.seek 37 @10:32:12.27 Selecting Cartridge 36 @10:32:12.57 SC.seek 36 @10:32:13.06 LyS2.seek :locked @10:32:13.19 PV.seek :locked @10:32:13.32 AirS.seek :locked @10:32:17.39 Powering cartridge bus @10:32:19.52 Gate.setIR 1750,5000,0,6 @10:32:19.65 Gate.setIR 0,5000,0,6 @10:32:19.69 Loaded Archive Cartridge @10:32:19.70 Gate.power :cartridge,:OFF @10:32:39.20 -> Cmd.startFiltering @10:32:39.43 Duration of filtering limited to 1:05:00 @10:32:39.68 Priming sample loop w/100ml, bypass w/10ml @10:32:39.75 SP.setPosition! 0ml @10:32:39.79 Intake.open @10:32:39.81 Exhaust.open @10:32:40.66 Sea @-42.9psia @10:32:40.68 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @10:32:46.21 Vacuum tare is 24% of full scale @10:32:46.22 PB.open @10:32:46.25 Exhaust.close @10:32:47.31 Sea @-42.9psia @10:32:47.39 SP.reconfigure SPprime @10:32:47.46 SP.seek 10ml,1:21 @10:32:58.33 SP.setPosition! 0ml @10:32:58.37 PB.close @10:32:58.40 Exhaust.open @10:32:59.18 Sea @-42.9psia @10:32:59.26 SP.reconfigure SPprime @10:32:59.33 SP.seek 100ml,8:51 @10:34:53.49 Intake.close @10:34:54.26 Pressurizing cartridge to 26psi (try #1 of 3) @10:34:54.27 SP.setPosition! 0ml @10:34:54.33 PB.open @10:34:55.09 TV.seek :crack @10:34:55.88 TV.seek :clear @10:34:56.68 Intake.open @10:34:58.17 PB.close @10:34:58.88 Exhaust.close @10:34:59.63 Sea @-42.9psia @10:34:59.72 SP.reconfigure SPcharge @10:34:59.78 SP.seek 2.3ml,42 seconds @10:35:06.97 Cartridge pressurized with 0.239ml to 26.7psi @10:35:07.20 delay 3 seconds @10:35:10.27 Cartridge at 23.1psi 3 seconds later @10:35:10.35 TV.seek :cartridge @10:35:12.17 TV.seek :clear @10:35:13.99 Sampling 1000.0ml @10:35:14.00 SP.setPosition! 0ml @10:35:14.06 Exhaust.open @10:35:14.84 Sea @-42.9psia @10:35:14.93 SP.reconfigure SPsample @10:35:14.99 SP.seek 1000ml,1:23:53 @10:35:15.95 Cartridge 36 sampling 0/1000ml at 0.17ml/s with 6.4psi vacuum @10:36:15.96 Cartridge 36 sampling 22/1000ml at 0.34ml/s with 24.4psi vacuum @10:37:15.96 Cartridge 36 sampling 41/1000ml at 0.31ml/s with 21.0psi vacuum @10:38:15.96 Cartridge 36 sampling 56/1000ml at 0.21ml/s with 27.0psi vacuum @10:39:15.96 Cartridge 36 sampling 69/1000ml at 0.20ml/s with 26.5psi vacuum @10:39:30.93 Flow averaged 0.29ml/s over 4:12 @10:39:30.95 Waiting up to 21 seconds for vacuum to reduce from 31.3 to 18.0psi @10:39:31.57 Relieved excess vacuum in 5.5 seconds @10:39:31.60 SP.seek 1000ml,1:17:53 @10:39:41.64 Flow estimate reduced to 0.23ml/s @10:39:41.66 Waiting up to 20 seconds for vacuum to reduce from 30.9 to 18.0psi @10:39:42.28 Relieved excess vacuum in 7.6 seconds @10:39:42.31 SP.seek 1000ml,1:17:43 @10:39:46.33 Flow estimate reduced to 0.18ml/s @10:39:46.35 Intake.close @10:39:46.40 Exhaust.close @10:39:47.21 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @10:39:47.31 Sampled 74.5ml @10:39:47.39 TV.seek :bypass @10:39:49.00 TV.seek :clear @10:39:51.36 -> Cmd.startProcessing @10:39:51.61 PV.seek :reagent @10:39:52.47 Sample loop closed. Ready to Deliver Reagents @10:39:52.49 ArS.seek :sealed @10:39:57.01 ArS.seek :evac @10:40:10.95 delay 20 seconds @10:40:30.99 ArS.seek :clear @10:40:48.71 RNAlater soak for 10:00 @10:50:48.74 ArS.seek :tVent @10:50:51.31 delay 1 second @10:50:52.34 ArS.seek :evac @10:51:07.96 delay 10 seconds @10:51:18.00 Sample preserved and evacuated @10:51:19.11 -> Cmd.stop @10:51:19.26 PV.seek :locked @10:51:20.15 PinPort.seek :clear @10:51:20.29 ArS.seek :clear @10:51:20.47 LyS2.seek :clear @10:51:20.60 AirS.seek :clear @10:51:38.04 ArS.coast @10:51:38.09 LyS1.coast @10:51:38.14 LyS2.coast @10:51:38.19 AirS.coast @10:51:38.24 TV.coast @10:51:38.29 SP.coast @10:51:38.34 SC.coast @10:51:38.39 PV.coast @10:51:38.44 PinPort.coast @10:51:38.61 Gate.power :motor,:OFF @10:51:38.63 Gate.power :main,:OFF @10:51:38.65 Gate.power :core,:OFF @10:51:38.67 Gate.power :analytic1,:OFF @10:51:38.69 Gate.power :analytic2,:OFF @10:51:38.71 Gate.power :analytic3,:OFF @10:51:38.73 Safely stopped and ready to power off ##-- file closed 2018-08-27T18:22:32.723Z