##-- file opened 2022-05-28T18:41:49.748Z
@11:41:51.22 -> showStatus
@11:41:52.84 -> Cmd.loadCartridge -6
@11:41:52.91 Gate.power :main,:ON,limit to 5000mA
@11:41:52.92 Gate.power :analytic1,:ON,limit to 5000mA
@11:41:52.95 Gate.power :analytic2,:ON,limit to 5000mA
@11:41:52.98 Gate.power :analytic3,:ON,limit to 5000mA
@11:41:53.12 Powering core bus
@11:41:55.38 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@11:41:55.83 <LRAUV> Archive.configure :default
@11:41:55.97 Air.configure :default
@11:41:56.08 Lysis1.configure :default
@11:41:56.18 Lysis2.configure :default
@11:41:56.42 Plunge.configure :default
@11:41:56.68 SamplePump.configure :default
@11:41:57.01 Toroid.configure :default
@11:41:57.29 Carousel.configure :default
@11:41:57.59 Pressure.configure :default
@11:41:57.82 ExtraPump.configure :default
@11:41:57.92 PinPort.configure :default
@11:41:57.99 All elf controllers running firmware version 4.79
@11:41:58.01 Gate.power :motor,:ON,limit to 5000mA
@11:41:58.23 <Archive_home> Archive.in :default
@11:41:58.45 Archive.home.jog 0
@11:41:58.59 <Toroid_home> Toroid.in :default
@11:41:58.66 <Plunge_home> Plunge.in :default
@11:41:58.70 <Lysis1_home> Lysis1.in :default
@11:41:58.77 <Air_home> Air.in :default
@11:41:58.82 <Lysis2_home> Lysis2.in :default
@11:41:58.88 <PinPort_home> PinPort.in :default
@11:41:59.02 <Toroid_home> Toroid.home.jog 0
@11:41:59.08 <Plunge_home> Plunge.home.jog 0
@11:41:59.11 <Lysis2_home> Lysis2.home.jog 0
@11:41:59.13 <Lysis1_home> Lysis1.home.jog 0
@11:41:59.17 <Air_home> Air.home.jog 0
@11:41:59.22 <PinPort_home> PinPort.home.jog 0
@11:41:59.54 <Archive_home> Archive.in :homing
@11:41:59.81 <Plunge_home> Plunge.in :homing
@11:41:59.88 <Archive_home> Archive.jog 1388
@11:41:59.94 <Toroid_home> Toroid.in :homing
@11:41:59.96 <Lysis2_home> Lysis2.in :homing
@11:42:00.15 <Plunge_home> Plunge.jog 1540
@11:42:00.18 <Toroid_home> Toroid.jog 635
@11:42:00.19 <Lysis2_home> Lysis2.jog 1393
@11:42:00.40 <PinPort_home> PinPort.in :homing
@11:42:00.44 <Lysis1_home> Lysis1.in :homing
@11:42:00.47 <Air_home> Air.in :homing
@11:42:00.59 <PinPort_home> PinPort.jog -106
@11:42:00.64 <Lysis1_home> Lysis1.jog 1380
@11:42:00.68 <Air_home> Air.jog 1384
@11:42:00.88 <PinPort_home> PinPort.setRawPosition! 0
@11:42:00.92 PinPort.seek :home
@11:42:01.01 PinPort.in :default
@11:42:01.19 <Archive_home> Archive.setRawPosition! 50
@11:42:01.24 Archive.seek :home
@11:42:01.44 <Lysis2_home> Lysis2.setRawPosition! 50
@11:42:01.46 <Plunge_home> Plunge.setRawPosition! 50
@11:42:01.56 Plunge.seek :home
@11:42:01.60 <Lysis2_home> Lysis2.seek :home
@11:42:01.74 <Archive_home> Archive.in :default
@11:42:01.88 <Lysis1_home> Lysis1.setRawPosition! 50
@11:42:01.94 Lysis1.seek :home
@11:42:02.03 <Plunge_home> Plunge.in :default
@11:42:02.05 <Lysis2_home> Lysis2.in :default
@11:42:02.08 <Air_home> Air.setRawPosition! 50
@11:42:02.24 Air.seek :home
@11:42:02.32 <Lysis1_home> Lysis1.in :default
@11:42:02.42 <Toroid_home> Toroid.setRawPosition! 0
@11:42:02.47 Toroid.seek :home
@11:42:02.58 Toroid.in :default
@11:42:02.60 <Air_home> Air.in :default
@11:42:03.03 <LRAUV> Toroid.seek :clear
@11:42:04.65 Carousel.in :default
@11:42:04.71 Carousel.home.jog 0
@11:42:05.24 Carousel.in :default
@11:42:05.30 Carousel.seek 52
@11:42:05.46 Selecting Cartridge 40
@11:42:05.71 Carousel.seek 40
@11:42:08.44 Lysis1.in :fast
@11:42:08.50 Lysis1.seek :locked
@11:42:11.10 <Plunge> Plunge.seek :locked
@11:42:11.23 <Air> Air.in :fast
@11:42:11.29 Air.seek :locked
@11:42:13.75 <LRAUV> Powering cartridge bus
@11:42:16.36 Gate.setIR 1750,5000,0,6
@11:42:16.48 Gate.setIR 0,5000,0,6
@11:42:16.54 Gate.power :cartridge,:OFF
@11:42:17.07 Mounted archive Cartridge
@11:42:32.43 -> Cmd.startFiltering
92.07@11:42:32.68 <FILTERING> Preparing to filter cartridge 40
@11:42:33.33 Priming sample loop w/100ml, bypass w/10ml
@11:42:33.34 SamplePump.setPosition! 0ml
@11:42:33.39 Bypass.open
@11:42:34.86 Intake.open
@11:42:35.61 Sea 17.6m deep (40.7psia)
@11:42:35.63 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@11:42:38.20 Vacuum tare is 54% of full scale
@11:42:38.34 <closeIntake> Intake.close
@11:42:38.39 <closeBypass> Bypass.close
@11:42:39.13 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 40.7psia
@11:42:39.15 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@11:42:43.72 Bypass.open
@11:42:44.38 Intake.open
@11:42:45.83 Pressure.in :default
@11:42:45.89 Pressure.hold
@11:42:46.07 SamplePump.in :prime
@11:42:46.13 SamplePump.seek 10ml,59 seconds
@11:42:54.76 Pressure.stop
@11:42:54.81 SamplePump.setPosition! 0ml
@11:42:54.86 Exhaust.open
@11:42:56.32 <closeBypass> Bypass.close
@11:42:57.81 <FILTERING> Pressure.in :default
@11:42:57.87 Pressure.hold
@11:42:57.99 Sea 17.7m deep (40.8psia)
@11:42:58.13 SamplePump.in :prime
@11:42:58.20 SamplePump.seek 100ml,5:16
@11:44:32.71 Pressure.stop
@11:44:32.77 Bypass.open
@11:44:33.48 Intake.close
@11:44:34.24 Toroid.in :hold
@11:44:34.30 Toroid.seek :crack
@11:44:35.81 Toroid.seek :cartridge
@11:44:36.90 Toroid.in :default
@11:44:36.96 Toroid.seek :clear
@11:44:38.93 <closeExhaust> Exhaust.close
@11:44:38.99 <closeBypass> Bypass.close
@11:44:39.73 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 40.8psia
@11:44:39.75 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@11:44:44.37 Sampling 1000.0ml
@11:44:44.39 SamplePump.setPosition! 0ml
@11:44:44.45 Exhaust.open
@11:44:45.17 Intake.open
@11:44:45.95 Pressure.in :default
@11:44:46.01 Pressure.hold
@11:44:46.13 Sea 17.8m deep (41.0psia)
@11:44:46.31 SamplePump.in :sample
@11:44:46.38 SamplePump.seek 1000ml,1:23:48
@11:44:48.99 <sampling> Cartridge 40 filtering 1/1000ml at 0.42ml/s with 27.9psi vacuum 13.8m deep (35.1psia)
@11:45:48.98 Cartridge 40 filtering 27/1000ml at 0.39ml/s with 22.3psi vacuum 17.7m deep (40.9psia)
@11:46:48.99 Cartridge 40 filtering 51/1000ml at 0.38ml/s with 24.8psi vacuum 15.4m deep (37.4psia)
@11:47:48.98 Cartridge 40 filtering 74/1000ml at 0.39ml/s with 25.9psi vacuum 15.4m deep (37.5psia)
@11:48:48.99 Cartridge 40 filtering 96/1000ml at 0.37ml/s with 24.9psi vacuum 15.3m deep (37.4psia)
@11:49:48.98 Cartridge 40 filtering 117/1000ml at 0.32ml/s with 23.5psi vacuum 16.5m deep (39.1psia)
@11:50:48.99 Cartridge 40 filtering 138/1000ml at 0.32ml/s with 20.9psi vacuum 18.0m deep (41.2psia)
@11:51:48.98 Cartridge 40 filtering 157/1000ml at 0.35ml/s with 22.4psi vacuum 17.7m deep (40.9psia)
@11:52:48.99 Cartridge 40 filtering 177/1000ml at 0.34ml/s with 22.6psi vacuum 18.2m deep (41.6psia)
@11:53:48.98 Cartridge 40 filtering 196/1000ml at 0.31ml/s with 24.0psi vacuum 16.0m deep (38.3psia)
@11:54:48.99 Cartridge 40 filtering 214/1000ml at 0.30ml/s with 25.9psi vacuum 15.9m deep (38.2psia)
@11:55:48.98 Cartridge 40 filtering 232/1000ml at 0.30ml/s with 23.4psi vacuum 15.7m deep (38.0psia)
@11:56:48.99 Cartridge 40 filtering 250/1000ml at 0.28ml/s with 26.3psi vacuum 15.7m deep (37.9psia)
@11:57:48.98 Cartridge 40 filtering 268/1000ml at 0.31ml/s with 24.5psi vacuum 15.0m deep (36.9psia)
@11:58:48.99 Cartridge 40 filtering 285/1000ml at 0.26ml/s with 26.0psi vacuum 14.9m deep (36.7psia)
@11:59:48.98 Cartridge 40 filtering 302/1000ml at 0.31ml/s with 25.0psi vacuum 19.1m deep (43.0psia)
@12:00:48.99 Cartridge 40 filtering 319/1000ml at 0.28ml/s with 22.9psi vacuum 16.8m deep (39.5psia)
@12:01:48.98 Cartridge 40 filtering 335/1000ml at 0.26ml/s with 24.3psi vacuum 16.3m deep (38.8psia)
@12:02:48.99 Cartridge 40 filtering 351/1000ml at 0.25ml/s with 21.1psi vacuum 19.7m deep (43.8psia)
@12:03:48.98 Cartridge 40 filtering 367/1000ml at 0.29ml/s with 24.0psi vacuum 17.8m deep (41.0psia)
@12:04:48.99 Cartridge 40 filtering 383/1000ml at 0.25ml/s with 26.1psi vacuum 17.3m deep (40.3psia)
@12:05:48.98 Cartridge 40 filtering 398/1000ml at 0.25ml/s with 22.3psi vacuum 18.8m deep (42.5psia)
@12:06:48.99 Cartridge 40 filtering 413/1000ml at 0.24ml/s with 25.2psi vacuum 18.8m deep (42.4psia)
@12:07:48.98 Cartridge 40 filtering 428/1000ml at 0.22ml/s with 25.6psi vacuum 16.0m deep (38.4psia)
@12:08:48.99 Cartridge 40 filtering 442/1000ml at 0.24ml/s with 22.0psi vacuum 15.3m deep (37.4psia)
@12:09:48.98 Cartridge 40 filtering 456/1000ml at 0.21ml/s with 24.8psi vacuum 15.8m deep (38.0psia)
@12:10:48.99 Cartridge 40 filtering 471/1000ml at 0.23ml/s with 22.6psi vacuum 17.9m deep (41.2psia)
@12:11:48.98 Cartridge 40 filtering 484/1000ml at 0.22ml/s with 27.0psi vacuum 17.7m deep (40.8psia)
@12:12:48.99 Cartridge 40 filtering 498/1000ml at 0.25ml/s with 24.5psi vacuum 19.0m deep (42.7psia)
@12:13:48.98 Cartridge 40 filtering 511/1000ml at 0.25ml/s with 23.7psi vacuum 19.6m deep (43.7psia)
@12:14:48.99 Cartridge 40 filtering 525/1000ml at 0.22ml/s with 22.4psi vacuum 18.9m deep (42.6psia)
@12:15:48.98 Cartridge 40 filtering 538/1000ml at 0.24ml/s with 20.1psi vacuum 20.4m deep (44.9psia)
@12:16:48.99 Cartridge 40 filtering 551/1000ml at 0.24ml/s with 26.6psi vacuum 19.0m deep (42.8psia)
@12:17:48.99 Cartridge 40 filtering 563/1000ml at 0.21ml/s with 22.6psi vacuum 17.6m deep (40.7psia)
@12:18:48.99 Cartridge 40 filtering 576/1000ml at 0.20ml/s with 25.1psi vacuum 16.7m deep (39.4psia)
@12:19:48.98 Cartridge 40 filtering 588/1000ml at 0.21ml/s with 25.6psi vacuum 19.3m deep (43.2psia)
@12:20:48.99 Cartridge 40 filtering 601/1000ml at 0.20ml/s with 25.4psi vacuum 18.9m deep (42.7psia)
@12:21:48.98 Cartridge 40 filtering 613/1000ml at 0.20ml/s with 23.0psi vacuum 17.9m deep (41.2psia)
@12:22:48.98 Cartridge 40 filtering 626/1000ml at 0.20ml/s with 26.1psi vacuum 17.2m deep (40.1psia)
@12:23:48.98 Cartridge 40 filtering 638/1000ml at 0.20ml/s with 23.1psi vacuum 18.9m deep (42.6psia)
@12:24:48.99 Cartridge 40 filtering 650/1000ml at 0.20ml/s with 25.2psi vacuum 18.1m deep (41.5psia)
@12:25:48.98 Cartridge 40 filtering 662/1000ml at 0.20ml/s with 24.9psi vacuum 17.2m deep (40.1psia)
@12:26:24.31 <FILTERING> Flow averaged 0.27ml/s over 41:38
@12:26:24.34 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi
@12:26:24.92 Relieved excess vacuum in .6 seconds
@12:26:24.95 SamplePump.seek 1000ml,28:02
@12:26:48.98 <sampling> Cartridge 40 filtering 674/1000ml at 0.20ml/s with 26.3psi vacuum 15.9m deep (38.2psia)
@12:27:48.98 Cartridge 40 filtering 686/1000ml at 0.20ml/s with 27.7psi vacuum 16.4m deep (38.9psia)
@12:28:18.71 <FILTERING> Flow averaged 0.20ml/s over 1:54
@12:28:18.75 Waiting up to 8 seconds for vacuum to reduce from 26.0 to 18.0psi
@12:28:19.32 Relieved excess vacuum in .6 seconds
@12:28:19.35 SamplePump.seek 1000ml,26:07
@12:28:48.98 <sampling> Cartridge 40 filtering 698/1000ml at 0.20ml/s with 26.0psi vacuum 16.9m deep (39.7psia)
@12:29:49.41 Cartridge 40 filtering 711/1000ml at 0.21ml/s with 22.1psi vacuum 19.3m deep (43.3psia)
@12:30:04.50 <FILTERING> Flow averaged 0.20ml/s over 1:46
@12:30:04.53 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi
@12:30:05.10 Relieved excess vacuum in .6 seconds
@12:30:05.13 SamplePump.seek 1000ml,24:21
@12:30:48.98 <sampling> Cartridge 40 filtering 723/1000ml at 0.20ml/s with 25.9psi vacuum 18.3m deep (41.8psia)
@12:31:29.92 <FILTERING> Flow averaged 0.20ml/s over 1:25
@12:31:29.95 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi
@12:31:30.52 Relieved excess vacuum in .6 seconds
@12:31:30.55 SamplePump.seek 1000ml,22:55
@12:31:48.99 <sampling> Cartridge 40 filtering 735/1000ml at 0.20ml/s with 25.5psi vacuum 16.4m deep (38.9psia)
@12:32:35.54 <FILTERING> Flow averaged 0.20ml/s over 1:05
@12:32:35.58 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi
@12:32:36.15 Relieved excess vacuum in .6 seconds
@12:32:36.18 SamplePump.seek 1000ml,21:50
@12:32:48.99 <sampling> Cartridge 40 filtering 747/1000ml at 0.20ml/s with 28.0psi vacuum 17.2m deep (40.1psia)
@12:33:26.72 <FILTERING> Flow averaged 0.20ml/s over 51 seconds
@12:33:26.76 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi
@12:33:27.33 Relieved excess vacuum in .6 seconds
@12:33:27.35 SamplePump.seek 1000ml,20:59
@12:33:48.99 <sampling> Cartridge 40 filtering 759/1000ml at 0.20ml/s with 26.3psi vacuum 17.1m deep (40.0psia)
@12:34:14.61 <FILTERING> Flow averaged 0.20ml/s over 48 seconds
@12:34:14.65 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi
@12:34:15.22 Relieved excess vacuum in .6 seconds
@12:34:15.25 SamplePump.seek 1000ml,20:11
@12:34:48.97 <sampling> Cartridge 40 filtering 771/1000ml at 0.20ml/s with 26.8psi vacuum 18.7m deep (42.3psia)
@12:34:57.69 <FILTERING> Flow averaged 0.20ml/s over 43 seconds
@12:34:57.73 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi
@12:34:58.30 Relieved excess vacuum in .6 seconds
@12:34:58.32 SamplePump.seek 1000ml,19:29
@12:35:48.99 <sampling> Cartridge 40 filtering 782/1000ml at 0.20ml/s with 24.1psi vacuum 18.4m deep (41.9psia)
@12:35:52.09 <FILTERING> Flow averaged 0.20ml/s over 54 seconds
@12:35:52.12 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi
@12:35:52.69 Relieved excess vacuum in .6 seconds
@12:35:52.72 SamplePump.seek 1000ml,18:34
@12:36:31.90 Flow averaged 0.20ml/s over 40 seconds
@12:36:31.94 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi
@12:36:32.50 Relieved excess vacuum in .6 seconds
@12:36:32.53 SamplePump.seek 1000ml,17:55
@12:36:48.98 <sampling> Cartridge 40 filtering 794/1000ml at 0.20ml/s with 27.3psi vacuum 16.2m deep (38.6psia)
@12:37:07.01 <FILTERING> Flow averaged 0.20ml/s over 35 seconds
@12:37:07.05 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi
@12:37:07.61 Relieved excess vacuum in .6 seconds
@12:37:07.64 SamplePump.seek 1000ml,17:21
@12:37:17.71 Flow averaged 0.20ml/s over 11 seconds
@12:37:17.74 <closeIntake> Intake.close
@12:37:17.78 <closeExhaust> Exhaust.close
@12:37:17.99 <FILTERING> Toroid.in :hold
@12:37:18.05 Toroid.seek :bypass
@12:37:19.67 Toroid.in :default
@12:37:19.74 Toroid.seek :clear
@12:37:21.35 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@12:37:21.37 Pressure.stop
@12:37:21.55 Sampled  799.9ml
@12:37:23.56 <LRAUV> -> Cmd.startProcessing
@12:37:24.02 <PROCESSING> Plunge.seek :reagent
@12:37:24.81 Sample loop closed. Ready to Deliver Reagents
@12:37:24.83 Archive.seek :sealed
@12:37:28.78 Archive.seek :evac
@12:37:42.82 delay 1:00
@12:38:42.84 Archive.seek :clear
@12:39:00.34 RNAlater soak for 10:00
@12:49:00.36 Archive.seek :tVent
@12:49:02.81 delay 1 second
@12:49:03.82 Archive.seek :evac
@12:49:19.33 delay 10 seconds
@12:49:29.35 Sample preserved and evacuated
@12:49:30.38 <LRAUV> -> Cmd.stop
@12:49:30.53 Carousel.seek 40
@12:49:30.71 Plunge.seek :locked
@12:49:31.64 <Archive> Archive.in :unlocking
@12:49:31.76 Archive.seek :clear
@12:49:31.82 <Air> Air.in :unlocking
@12:49:31.96 Air.seek :clear
@12:49:32.08 <Lysis1> Lysis1.in :unlocking
@12:49:32.17 Lysis1.seek :clear
@12:49:35.88 <LRAUV> Gate.power :motor,:OFF
@12:49:35.90 Gate.power :core,:OFF
@12:49:35.92 Gate.power :analytic1,:OFF
@12:49:35.94 Gate.power :analytic2,:OFF
@12:49:35.96 Gate.power :analytic3,:OFF
@12:49:35.99 Gate.power :main,:OFF
@12:49:36.01 Safely stopped and ready to power off
##-- file closed 2022-05-28T20:01:22.030Z

##-- file opened 2022-05-28T20:01:22.033Z
@13:01:23.10 -> showStatus
@13:01:25.12 -> Cmd.loadCartridge -10
@13:01:25.19 Gate.power :main,:ON,limit to 5000mA
@13:01:25.21 Gate.power :analytic1,:ON,limit to 5000mA
@13:01:25.24 Gate.power :analytic2,:ON,limit to 5000mA
@13:01:25.26 Gate.power :analytic3,:ON,limit to 5000mA
@13:01:25.40 Powering core bus
@13:01:27.67 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@13:01:28.13 <LRAUV> Archive.configure :default
@13:01:28.25 Air.configure :default
@13:01:28.36 Lysis1.configure :default
@13:01:28.46 Lysis2.configure :default
@13:01:28.59 Plunge.configure :default
@13:01:28.71 SamplePump.configure :default
@13:01:28.92 Toroid.configure :default
@13:01:29.21 Carousel.configure :default
@13:01:29.49 Pressure.configure :default
@13:01:29.78 ExtraPump.configure :default
@13:01:30.01 PinPort.configure :default
@13:01:30.17 All elf controllers running firmware version 4.79
@13:01:30.24 Gate.power :motor,:ON,limit to 5000mA
@13:01:30.43 <Archive_home> Archive.in :default
@13:01:30.73 Archive.home.jog 0
@13:01:30.86 <Toroid_home> Toroid.in :default
@13:01:30.93 <Plunge_home> Plunge.in :default
@13:01:30.96 <Lysis1_home> Lysis1.in :default
@13:01:31.03 <PinPort_home> PinPort.in :default
@13:01:31.07 <Lysis2_home> Lysis2.in :default
@13:01:31.12 <Air_home> Air.in :default
@13:01:31.31 <Toroid_home> Toroid.home.jog 0
@13:01:31.36 <Lysis1_home> Lysis1.home.jog 0
@13:01:31.38 <Plunge_home> Plunge.home.jog 0
@13:01:31.40 <Lysis2_home> Lysis2.home.jog 0
@13:01:31.44 <PinPort_home> PinPort.home.jog 0
@13:01:31.45 <Air_home> Air.home.jog 0
@13:01:31.96 <Archive_home> Archive.in :homing
@13:01:32.20 <Toroid_home> Toroid.in :homing
@13:01:32.24 <Plunge_home> Plunge.in :homing
@13:01:32.27 <Archive_home> Archive.jog 1390
@13:01:32.32 <PinPort_home> PinPort.in :homing
@13:01:32.35 <Lysis1_home> Lysis1.in :homing
@13:01:32.38 <Air_home> Air.in :homing
@13:01:32.43 <Lysis2_home> Lysis2.in :homing
@13:01:32.65 <Plunge_home> Plunge.jog 1555
@13:01:32.69 <Toroid_home> Toroid.jog 642
@13:01:32.72 <PinPort_home> PinPort.jog -107
@13:01:32.75 <Lysis1_home> Lysis1.jog 1380
@13:01:32.77 <Air_home> Air.jog 1384
@13:01:32.80 <Lysis2_home> Lysis2.jog 1397
@13:01:33.10 <PinPort_home> PinPort.setRawPosition! 0
@13:01:33.15 PinPort.seek :home
@13:01:33.24 PinPort.in :default
@13:01:33.66 <Archive_home> Archive.setRawPosition! 50
@13:01:33.71 Archive.seek :home
@13:01:33.99 Archive.in :default
@13:01:34.12 <Plunge_home> Plunge.setRawPosition! 50
@13:01:34.14 <Lysis1_home> Lysis1.setRawPosition! 50
@13:01:34.17 <Air_home> Air.setRawPosition! 50
@13:01:34.19 <Lysis2_home> Lysis2.setRawPosition! 50
@13:01:34.30 <Plunge_home> Plunge.seek :home
@13:01:34.35 <Lysis1_home> Lysis1.seek :home
@13:01:34.40 <Lysis2_home> Lysis2.seek :home
@13:01:34.43 <Air_home> Air.seek :home
@13:01:34.73 <Plunge_home> Plunge.in :default
@13:01:34.84 <Lysis2_home> Lysis2.in :default
@13:01:34.87 <Lysis1_home> Lysis1.in :default
@13:01:34.90 <Air_home> Air.in :default
@13:01:35.09 <Toroid_home> Toroid.setRawPosition! 0
@13:01:35.13 Toroid.seek :home
@13:01:35.23 Toroid.in :default
@13:01:35.38 <LRAUV> Toroid.seek :clear
@13:01:37.00 Carousel.in :default
@13:01:37.06 Carousel.home.jog 0
@13:01:37.54 Carousel.in :default
@13:01:37.60 Carousel.seek 40
@13:01:37.76 Selecting Cartridge 39
@13:01:38.02 Carousel.seek 39
@13:01:38.51 Lysis1.in :fast
@13:01:38.57 Lysis1.seek :locked
@13:01:41.17 <Plunge> Plunge.seek :locked
@13:01:41.30 <Air> Air.in :fast
@13:01:41.36 Air.seek :locked
@13:01:43.82 <LRAUV> Powering cartridge bus
@13:01:46.43 Gate.setIR 1750,5000,0,6
@13:01:46.55 Gate.setIR 0,5000,0,6
@13:01:46.60 PuckHeater.configure :default
@13:01:46.66 Gate.power :heat,:ON,limit to 5000mA
@13:01:47.34 Mounted lysis Cartridge board#Q05034
@13:01:47.38 Ignoring this cartridge's HeatingAccelInCarousel heat loss curve
@13:02:05.52 -> Cmd.startFiltering
@13:02:06.14 <FILTERING> Preparing to filter cartridge 39
@13:02:06.40 Priming sample loop w/100ml, bypass w/10ml
@13:02:06.42 SamplePump.setPosition! 0ml
@13:02:06.46 Bypass.open
@13:02:07.93 Intake.open
@13:02:08.66 Sea 19.0m deep (42.8psia)
@13:02:08.68 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@13:02:11.24 Vacuum tare is 54% of full scale
@13:02:11.38 <closeIntake> Intake.close
@13:02:11.44 <closeBypass> Bypass.close
@13:02:12.13 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 42.7psia
@13:02:12.15 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@13:02:16.73 Toroid pressure rose 1% to 43.1psia
@13:02:16.74 Bypass.open
@13:02:17.41 Intake.open
@13:02:18.84 Pressure.in :default
@13:02:18.90 Pressure.hold
@13:02:19.07 SamplePump.in :prime
@13:02:19.15 SamplePump.seek 10ml,59 seconds
@13:02:27.76 Pressure.stop
@13:02:27.81 SamplePump.setPosition! 0ml
@13:02:27.86 Exhaust.open
@13:02:29.32 <closeBypass> Bypass.close
@13:02:30.80 <FILTERING> Pressure.in :default
@13:02:30.86 Pressure.hold
@13:02:30.98 Sea 19.2m deep (43.1psia)
@13:02:31.11 SamplePump.in :prime
@13:02:31.18 SamplePump.seek 100ml,5:16
@13:04:05.54 Pressure.stop
@13:04:05.60 Bypass.open
@13:04:06.27 Intake.close
@13:04:07.00 Toroid.in :hold
@13:04:07.06 Toroid.seek :crack
@13:04:08.57 Toroid.seek :cartridge
@13:04:09.71 Toroid.in :default
@13:04:09.77 Toroid.seek :clear
@13:04:11.74 <closeExhaust> Exhaust.close
@13:04:11.79 <closeBypass> Bypass.close
@13:04:12.53 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 42.5psia
@13:04:12.55 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@13:04:17.12 Cartridge pressure fell 2% to 41.4psia
@13:04:17.19 Sampling 1000.0ml
@13:04:17.21 SamplePump.setPosition! 0ml
@13:04:17.27 Exhaust.open
@13:04:18.01 Intake.open
@13:04:18.81 Pressure.in :default
@13:04:18.87 Pressure.hold
@13:04:19.03 Sea 18.8m deep (42.5psia)
@13:04:19.17 SamplePump.in :sample
@13:04:19.24 SamplePump.seek 1000ml,1:23:48
@13:04:21.84 <sampling> Cartridge 39 filtering 1/1000ml at 0.39ml/s with 22.9psi vacuum 19.3m deep (43.3psia)
@13:05:21.83 Cartridge 39 filtering 26/1000ml at 0.41ml/s with 24.3psi vacuum 19.6m deep (43.6psia)
@13:06:21.84 Cartridge 39 filtering 49/1000ml at 0.36ml/s with 25.1psi vacuum 18.1m deep (41.4psia)
@13:07:21.83 Cartridge 39 filtering 72/1000ml at 0.39ml/s with 24.5psi vacuum 17.1m deep (40.0psia)
@13:08:21.84 Cartridge 39 filtering 94/1000ml at 0.37ml/s with 22.7psi vacuum 20.3m deep (44.7psia)
@13:09:21.83 Cartridge 39 filtering 115/1000ml at 0.36ml/s with 21.1psi vacuum 19.6m deep (43.6psia)
@13:10:21.84 Cartridge 39 filtering 135/1000ml at 0.31ml/s with 24.1psi vacuum 16.7m deep (39.4psia)
@13:11:21.83 Cartridge 39 filtering 155/1000ml at 0.30ml/s with 23.7psi vacuum 17.4m deep (40.4psia)
@13:12:21.84 Cartridge 39 filtering 174/1000ml at 0.34ml/s with 23.2psi vacuum 20.8m deep (45.4psia)
@13:13:21.83 Cartridge 39 filtering 192/1000ml at 0.34ml/s with 25.8psi vacuum 19.8m deep (43.9psia)
@13:14:21.84 Cartridge 39 filtering 211/1000ml at 0.30ml/s with 22.8psi vacuum 17.4m deep (40.5psia)
@13:15:21.83 Cartridge 39 filtering 229/1000ml at 0.28ml/s with 26.6psi vacuum 18.6m deep (42.2psia)
@13:16:21.84 Cartridge 39 filtering 246/1000ml at 0.32ml/s with 22.5psi vacuum 22.5m deep (48.0psia)
@13:17:21.83 Cartridge 39 filtering 263/1000ml at 0.27ml/s with 23.7psi vacuum 20.8m deep (45.4psia)
@13:18:21.84 Cartridge 39 filtering 280/1000ml at 0.28ml/s with 22.6psi vacuum 17.8m deep (41.0psia)
@13:19:21.83 Cartridge 39 filtering 297/1000ml at 0.26ml/s with 25.7psi vacuum 18.6m deep (42.2psia)
@13:20:21.84 Cartridge 39 filtering 313/1000ml at 0.28ml/s with 27.3psi vacuum 18.9m deep (42.6psia)
@13:21:21.83 Cartridge 39 filtering 329/1000ml at 0.27ml/s with 24.2psi vacuum 19.3m deep (43.2psia)
@13:22:21.83 Cartridge 39 filtering 345/1000ml at 0.25ml/s with 24.3psi vacuum 19.0m deep (42.7psia)
@13:23:21.83 Cartridge 39 filtering 360/1000ml at 0.25ml/s with 23.1psi vacuum 19.2m deep (43.1psia)
@13:24:21.84 Cartridge 39 filtering 376/1000ml at 0.28ml/s with 24.0psi vacuum 19.7m deep (43.9psia)
@13:25:21.83 Cartridge 39 filtering 391/1000ml at 0.23ml/s with 24.3psi vacuum 19.6m deep (43.7psia)
@13:26:21.84 Cartridge 39 filtering 405/1000ml at 0.23ml/s with 24.8psi vacuum 20.4m deep (44.8psia)
@13:27:21.83 Cartridge 39 filtering 420/1000ml at 0.23ml/s with 23.2psi vacuum 20.5m deep (45.0psia)
@13:28:21.84 Cartridge 39 filtering 434/1000ml at 0.26ml/s with 24.3psi vacuum 19.7m deep (43.8psia)
@13:29:21.83 Cartridge 39 filtering 448/1000ml at 0.23ml/s with 23.4psi vacuum 21.2m deep (46.1psia)
@13:30:21.84 Cartridge 39 filtering 462/1000ml at 0.22ml/s with 23.5psi vacuum 20.4m deep (44.9psia)
@13:31:21.83 Cartridge 39 filtering 475/1000ml at 0.25ml/s with 24.1psi vacuum 21.2m deep (46.0psia)
@13:32:21.84 Cartridge 39 filtering 489/1000ml at 0.21ml/s with 24.9psi vacuum 20.9m deep (45.6psia)
@13:33:21.83 Cartridge 39 filtering 502/1000ml at 0.21ml/s with 25.0psi vacuum 19.7m deep (43.8psia)
@13:34:21.89 Cartridge 39 filtering 515/1000ml at 0.21ml/s with 27.4psi vacuum 16.7m deep (39.4psia)
@13:35:21.84 Cartridge 39 filtering 528/1000ml at 0.24ml/s with 26.1psi vacuum 19.9m deep (44.1psia)
@13:36:21.84 Cartridge 39 filtering 540/1000ml at 0.20ml/s with 25.2psi vacuum 17.5m deep (40.6psia)
@13:37:21.84 Cartridge 39 filtering 553/1000ml at 0.22ml/s with 20.7psi vacuum 20.1m deep (44.4psia)
@13:38:21.84 Cartridge 39 filtering 565/1000ml at 0.21ml/s with 20.0psi vacuum 19.9m deep (44.1psia)
@13:39:21.83 Cartridge 39 filtering 578/1000ml at 0.20ml/s with 25.7psi vacuum 19.0m deep (42.8psia)
@13:40:21.84 Cartridge 39 filtering 590/1000ml at 0.20ml/s with 24.4psi vacuum 21.1m deep (45.8psia)
@13:41:21.83 Cartridge 39 filtering 602/1000ml at 0.20ml/s with 27.3psi vacuum 19.6m deep (43.7psia)
@13:42:21.84 Cartridge 39 filtering 614/1000ml at 0.20ml/s with 25.8psi vacuum 19.2m deep (43.1psia)
@13:43:00.41 <FILTERING> Flow averaged 0.27ml/s over 38:42
@13:43:00.45 Waiting up to 8 seconds for vacuum to reduce from 26.0 to 18.0psi
@13:43:01.02 Relieved excess vacuum in .6 seconds
@13:43:01.05 SamplePump.seek 1000ml,31:58
@13:43:21.83 <sampling> Cartridge 39 filtering 626/1000ml at 0.20ml/s with 24.9psi vacuum 20.5m deep (44.9psia)
@13:44:21.84 Cartridge 39 filtering 639/1000ml at 0.20ml/s with 26.5psi vacuum 19.4m deep (43.4psia)
@13:44:57.61 <FILTERING> Flow averaged 0.20ml/s over 1:57
@13:44:57.64 Waiting up to 8 seconds for vacuum to reduce from 26.0 to 18.0psi
@13:44:58.21 Relieved excess vacuum in .6 seconds
@13:44:58.25 SamplePump.seek 1000ml,30:00
@13:45:21.83 <sampling> Cartridge 39 filtering 651/1000ml at 0.21ml/s with 26.5psi vacuum 20.4m deep (44.8psia)
@13:46:22.26 Cartridge 39 filtering 663/1000ml at 0.20ml/s with 25.2psi vacuum 20.2m deep (44.6psia)
@13:46:58.09 <FILTERING> Flow averaged 0.20ml/s over 2:00
@13:46:58.13 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@13:46:58.70 Relieved excess vacuum in .6 seconds
@13:46:58.73 SamplePump.seek 1000ml,27:59
@13:47:21.83 <sampling> Cartridge 39 filtering 675/1000ml at 0.20ml/s with 26.4psi vacuum 20.1m deep (44.3psia)
@13:48:21.84 Cartridge 39 filtering 687/1000ml at 0.20ml/s with 21.8psi vacuum 21.5m deep (46.5psia)
@13:48:26.30 <FILTERING> Flow averaged 0.20ml/s over 1:28
@13:48:26.34 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi
@13:48:26.92 Relieved excess vacuum in .6 seconds
@13:48:26.95 SamplePump.seek 1000ml,26:30
@13:49:21.84 <sampling> Cartridge 39 filtering 699/1000ml at 0.20ml/s with 26.9psi vacuum 22.5m deep (47.9psia)
@13:49:35.07 <FILTERING> Flow averaged 0.20ml/s over 1:09
@13:49:35.11 Waiting up to 14 seconds for vacuum to reduce from 28.5 to 18.0psi
@13:49:35.68 Relieved excess vacuum in .6 seconds
@13:49:35.71 SamplePump.seek 1000ml,25:21
@13:50:14.63 Flow averaged 0.20ml/s over 39 seconds
@13:50:14.67 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi
@13:50:15.24 Relieved excess vacuum in .6 seconds
@13:50:15.27 SamplePump.seek 1000ml,24:42
@13:50:21.83 <sampling> Cartridge 39 filtering 711/1000ml at 0.20ml/s with 26.0psi vacuum 22.2m deep (47.6psia)
@13:51:02.86 <FILTERING> Flow averaged 0.20ml/s over 48 seconds
@13:51:02.90 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi
@13:51:03.47 Relieved excess vacuum in .6 seconds
@13:51:03.50 SamplePump.seek 1000ml,23:54
@13:51:21.84 <sampling> Cartridge 39 filtering 723/1000ml at 0.21ml/s with 25.0psi vacuum 22.1m deep (47.4psia)
@13:51:47.23 <FILTERING> Flow averaged 0.20ml/s over 44 seconds
@13:51:47.27 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@13:51:47.84 Relieved excess vacuum in .6 seconds
@13:51:47.87 SamplePump.seek 1000ml,23:10
@13:52:21.83 <sampling> Cartridge 39 filtering 735/1000ml at 0.20ml/s with 28.7psi vacuum 21.9m deep (47.0psia)
@13:52:30.14 <FILTERING> Flow averaged 0.20ml/s over 43 seconds
@13:52:30.17 Waiting up to 15 seconds for vacuum to reduce from 28.6 to 18.0psi
@13:52:30.74 Relieved excess vacuum in .6 seconds
@13:52:30.77 SamplePump.seek 1000ml,22:28
@13:53:08.16 Flow averaged 0.20ml/s over 38 seconds
@13:53:08.21 Waiting up to 16 seconds for vacuum to reduce from 29.2 to 18.0psi
@13:53:08.77 Relieved excess vacuum in .6 seconds
@13:53:08.80 SamplePump.seek 1000ml,21:50
@13:53:21.86 <sampling> Cartridge 39 filtering 747/1000ml at 0.20ml/s with 27.5psi vacuum 20.7m deep (45.3psia)
@13:53:32.04 <FILTERING> Flow averaged 0.20ml/s over 24 seconds
@13:53:32.06 <closeIntake> Intake.close
@13:53:32.12 <closeExhaust> Exhaust.close
@13:53:32.31 <FILTERING> Toroid.in :hold
@13:53:32.37 Toroid.seek :bypass
@13:53:33.99 Toroid.in :default
@13:53:34.06 Toroid.seek :clear
@13:53:35.67 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@13:53:35.69 Pressure.stop
@13:53:35.87 Sampled  748.6ml
@13:53:37.83 <LRAUV> -> Cmd.startProcessing
@13:53:39.27 <PROCESSING> SPR.command \"read_sequences\"
@13:53:39.31 SPR.command \"stats_on\"
@13:53:39.41 SPR.command \"start 0 1\"
@13:53:40.15 <SPRlogger> SPR.start /var/log/esp/spr22may2813h.spr.bz2
@13:53:42.03 <PROCESSING> Handoff.select :waste
@13:53:48.72 Syringe tare is 18% of full scale
@13:53:48.74 PinPort.in :locking
@13:53:48.81 PinPort.seek :locked
@13:53:49.29 <posArchive> Archive.in :fast
@13:53:49.34 <posLyS2> Lysis2.in :fast
@13:53:49.41 <PROCESSING> Lysis1.seek 1350uL
@13:53:49.48 <posArchive> Archive.seek :full
@13:53:49.54 <posLyS2> Lysis2.seek :full
@13:53:51.55 <PROCESSING> Plunge.seek :reagent
@13:53:52.42 Evacuating puck with filter bubble point of 50psi
@13:53:52.44 PinPort.seek :locked
@13:53:52.52 Handoff.select :waste
@13:53:59.60 Air syringe pumping up to 1 stroke to reach 8psi
@13:53:59.61 Air stroke #1 of 1
@13:53:59.71 Air.in :pressurize
@13:53:59.77 Air.seek :pressurized
@13:54:11.35 0.9psi after pressurize
@13:54:11.37 Air.in :compress
@13:54:11.44 Air.seek :evac
@13:54:15.53 0.9psi after compress
@13:54:28.11 -0.1psi after compress (stable)
@13:54:28.12 Air.in :default
@13:54:28.19 Air.hold
@13:54:28.37 at -0.1psi Opened to waste
@13:54:28.38 Handoff.select :blocked
@13:54:28.52 Archive syringe pumping up to 8 strokes to reach 50psi
@13:54:28.53 Archive stroke #1 of 8
@13:54:28.61 Archive.in :pressurize
@13:54:28.68 Archive.seek :pressurized
@13:54:41.54 0.9psi after pressurize
@13:54:41.56 Archive.in :compress
@13:54:41.63 Archive.seek :empty
@13:54:56.35 24.6psi after compress
@13:55:11.92 29.3psi after compress (stable)
@13:55:11.94 Archive.in :gulp
@13:55:12.02 Archive.seek :topVent
@13:55:19.18 29.2psi after gulp
@13:55:31.75 28.9psi after gulp (stable)
@13:55:31.76 Archive stroke #2 of 8
@13:55:31.85 Archive.in :pressurize
@13:55:31.92 Archive.seek :pressurized
@13:55:44.78 28.7psi after pressurize
@13:55:44.80 Archive.in :compress
@13:55:44.87 Archive.seek :empty
@13:55:59.55 33.1psi after compress
@13:56:12.13 34.1psi after compress (stable)
@13:56:12.15 Archive.in :gulp
@13:56:12.23 Archive.seek :topVent
@13:56:19.38 35.6psi after gulp
@13:56:31.95 36.2psi after gulp (stable)
@13:56:31.96 Archive stroke #3 of 8
@13:56:32.06 Archive.in :pressurize
@13:56:32.12 Archive.seek :pressurized
@13:56:44.98 36.4psi after pressurize
@13:56:45.00 Archive.in :compress
@13:56:45.10 Archive.seek :empty
@13:56:59.83 37.7psi after compress
@13:57:51.89 46.4psi after compress (stable)
@13:57:51.92 Archive.in :gulp
@13:57:51.99 Archive.seek :topVent
@13:57:59.15 48.8psi after gulp
@13:58:08.83 Reached 50.0 of 50.0psi after pumping 5190uL (3.00 strokes)
@13:58:08.84 Archive.in :default
@13:58:08.91 Archive.hold
@13:58:08.98 Handoff.select :waste
@13:58:29.66 delay 1:00
@13:59:29.67 Handoff.select :blocked
@13:59:52.70 Handoff.select :waste
@13:59:52.82 2.4psi at evacEnd
@13:59:52.84 Handoff.select :blocked
@13:59:52.97 Archive.in :fast
@13:59:53.03 Archive.seek :topVent
@13:59:59.66 Stable @0.7psi after Handoff blocked
@13:59:59.68 Archive syringe pumping up to 8 strokes to reach 31psi
@13:59:59.70 Archive stroke #1 of 8
@13:59:59.79 Archive.in :pressurize
@13:59:59.86 Archive.seek :pressurized
@14:00:12.73 2.0psi after pressurize
@14:00:12.75 Archive.in :compress
@14:00:12.82 Archive.seek :empty
@14:00:27.51 6.5psi after compress
@14:01:00.08 16.0psi after compress (stable)
@14:01:00.10 Archive.in :gulp
@14:01:00.18 Archive.seek :topVent
@14:01:07.34 17.1psi after gulp
@14:01:19.91 17.8psi after gulp (stable)
@14:01:19.92 Archive stroke #2 of 8
@14:01:20.01 Archive.in :pressurize
@14:01:20.07 Archive.seek :pressurized
@14:01:32.92 18.6psi after pressurize
@14:01:32.94 Archive.in :compress
@14:01:33.02 Archive.seek :empty
@14:01:47.72 21.0psi after compress
@14:02:16.79 28.6psi after compress (stable)
@14:02:16.81 Archive.in :gulp
@14:02:16.89 Archive.seek :topVent
@14:02:24.05 29.7psi after gulp
@14:02:36.63 30.5psi after gulp (stable)
@14:02:36.65 Archive stroke #3 of 8
@14:02:36.81 Reached 30.7 of 30.7psi after pumping 3460uL (2.00 strokes)
@14:02:36.82 Archive.in :default
@14:02:36.91 Archive.hold
@14:02:36.99 Handoff.select :waste
@14:02:44.16 delay 1:00
@14:03:44.17 Handoff.select :blocked
@14:04:07.85 Handoff.select :waste
@14:04:07.97 3.0psi at evacEnd
@14:04:07.99 Air.seek :fullVent
@14:04:09.42 Archive.in :fast
@14:04:09.48 Archive.seek :topVent
@14:04:09.55 Seawater evacuated, Presure above/below filter released. Lysis begins
@14:04:09.66 Lysis1.in :slow
@14:04:09.73 Lysis1.seek 1050uL
@14:04:39.75 delay 5 seconds
@14:04:44.77 Handoff.select :blocked
@14:04:44.95 Lysis1.seek 450uL
@14:05:44.91 delay 3 seconds
@14:05:48.01 Air.in mix with 1.40048 parts Lysis1
@14:05:48.45 <Airmixer> Air.seek :evac
@14:05:48.48 <PROCESSING> Lysis1.seek 300uL
@14:06:03.60 Lysis1.seek :empty
@14:06:33.70 Lysis buffer delivered @23.3psi
@14:06:33.71 Polling Can every 2:00
@14:06:34.26 PuckHeater.configure :default #ambient 13.8C
@14:06:34.33 PuckHeater.seek 80.1C
@14:07:43.59 PuckHeater.hold 2:00,80.1C
@14:09:43.82 Polling Can every 2:00
@14:09:44.35 PuckHeater.configure :default #ambient 13.8C
@14:09:44.42 PuckHeater.hold 30 seconds,79.1C
@14:10:14.69 Polling Can every 2:00
@14:10:15.23 PuckHeater.configure :default #ambient 13.8C
@14:10:15.29 PuckHeater.hold 30 seconds,78.6C
@14:10:45.51 Polling Can every 2:00
@14:10:46.06 PuckHeater.hold 10:00,78.1C
@14:20:47.16 Lysis complete. Syringe pressure @32.0psi
@14:20:47.18 Handoff.select :blocked
@14:20:47.24 Archive syringe pumping up to 5 strokes to reach 40psi
@14:20:47.25 Archive stroke #1 of 5
@14:20:47.34 Archive.in :pressurize
@14:20:47.41 Archive.seek :pressurized
@14:21:00.28 31.4psi after pressurize
@14:21:00.30 Archive.in :compress
@14:21:00.37 Archive.seek :empty
@14:21:07.18 Reached 45.0 of 40.0psi after pumping 1554uL (0.90 strokes)
@14:21:07.19 Archive.in :default
@14:21:07.26 Archive.hold
@14:21:07.38 delay 2 seconds
@14:21:09.40 Archive.in :fast
@14:21:09.46 Archive.seek :full
@14:21:16.00 Lysis1.seek 138uL
@14:21:29.87 delay 1 second
@14:21:30.89 <fillArchive> Archive.seek :full
@14:21:30.94 <PROCESSING> Lysis1.seek :empty
@14:21:44.91 Initial air pressure @53.9psi
@14:21:44.99 Lysis1.in :extract
@14:21:45.08 Lysis1.seek 600uL,nil
@14:21:49.02 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@14:21:49.03 Archive stroke #1 of 6
@14:21:49.12 Archive.in :pressurize
@14:21:49.21 Archive.seek :pressurized
@14:22:02.05 13.6psi after pressurize
@14:22:02.07 Archive.in :compress
@14:22:02.14 Archive.seek :empty
@14:22:07.16 Syringe::LysatePlungerStuck in PROCESSING -- at < 238uL during Lysis Syringe #1 (LyS1) move
@14:22:07.19 <PROCESSING> Lysis1.in :default
@14:22:07.26 Lysis1.seek 203uL
@14:22:08.14 <pump> Reached 26.0 of 25.0psi after pumping 1543uL (0.89 strokes)
@14:22:08.15 Archive.in :default
@14:22:08.23 Archive.hold
@14:22:09.07 <PROCESSING> Lysis1.in :extract
@14:22:09.13 Lysis1.seek 600uL,nil
@14:22:15.53 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@14:22:15.54 Archive stroke #1 of 6
@14:22:15.63 Archive.in :compress
@14:22:15.71 Archive.seek :empty
@14:22:16.32 Archive.in :default
@14:22:16.38 Archive.hold
@14:22:16.55 <PROCESSING> Initial 600uL lysate @15.8psi
@14:22:16.56 Handoff.select :blocked
@14:22:16.66 Initial air pressure @16.0psi
@14:22:16.73 Lysis1.seek 825uL,nil
@14:22:18.49 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@14:22:18.50 Archive stroke #1 of 6
@14:22:18.60 Archive.in :compress
@14:22:18.66 Archive.seek :empty
@14:22:24.43 Archive.in :default
@14:22:24.49 Archive.hold
@14:22:24.69 <PROCESSING> Recovered 825uL lysate @16.7psi
@14:22:24.72 Air.in :halfSpeed
@14:22:24.78 Air.seek :fullVent
@14:22:26.99 Puck Top Vented, bottom @0.1psi
@14:22:27.00 Handoff.select :waste
@14:22:27.12 delay 5 seconds
@14:22:32.19 Handoff open to waste, bottom @0.0psi
@14:22:32.20 Air.in :default
@14:22:32.27 Air.seek :evac
@14:22:33.68 Lysis2.in :DAmix
@14:22:33.79 Lysis1.in :DAmix
@14:22:34.23 <Lysis2mixer> Lysis2.seek 1480uL
@14:22:34.27 <PROCESSING> Lysis1.seek 575uL
@14:23:09.36 delay 5 seconds
@14:23:14.37 Handoff.select SPR
@14:23:14.50 SPR.command \"start 2 14\"
@14:23:15.95 SPR stabilized
@14:23:16.08 delay 10 seconds
@14:23:26.10 SPR.command \"start 1 0\"
@14:23:28.87 Waiting up to 4:00 for SPR to enter state 1
@14:25:31.20 SPR.command \"hold_on\"
@14:25:32.62 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@14:25:33.09 <Lysis2mixer> Lysis2.seek 1180uL
@14:25:33.17 <PROCESSING> Lysis1.seek 275uL
@14:26:15.19 delay 15 seconds
@14:26:30.21 SPR.command \"hold_off\"
@14:26:33.27 Waiting up to 38:00 for SPR to enter state 7
@14:47:23.10 <SPRlogger> SPRsummary:-6.23067,118.129,109.692,116.783 after 3380s
@14:54:00.33 <PROCESSING> SPR analysis completed
@14:54:07.66 <SPRlogger> Ended log spr22may2813h.spr.bz2
@14:54:07.68 <PROCESSING> SPR.command \"stats_off\"
@14:54:09.15 <LRAUV> -> Cmd.stop
@14:54:09.25 <closeHandoff> Handoff.select :close
@14:54:09.38 <LRAUV> Gate.power :heat,:OFF
@14:54:09.40 Gate.power :cartridge,:OFF
@14:54:09.49 Carousel.seek 39
@14:54:09.66 Plunge.seek :locked
@14:54:10.52 PinPort.in :unlocking
@14:54:10.59 PinPort.seek :clear
@14:54:11.13 <Archive> Archive.in :unlocking
@14:54:11.26 Archive.seek :clear
@14:54:11.30 <Air> Air.in :unlocking
@14:54:11.46 Air.seek :clear
@14:54:11.57 <Lysis1> Lysis1.in :unlocking
@14:54:11.66 <Lysis2> Lysis2.in :unlocking
@14:54:11.69 <Lysis1> Lysis1.seek :clear
@14:54:11.78 <Lysis2> Lysis2.seek :clear
@14:54:15.37 <LRAUV> Gate.power :motor,:OFF
@14:54:15.38 Gate.power :core,:OFF
@14:54:15.41 Gate.power :analytic1,:OFF
@14:54:15.43 Gate.power :analytic2,:OFF
@14:54:15.45 Gate.power :analytic3,:OFF
@14:54:15.48 Gate.power :main,:OFF
@14:54:15.50 Safely stopped and ready to power off
##-- file closed 2022-05-29T01:00:57.954Z

##-- file opened 2022-05-29T01:00:57.956Z
@18:00:59.03 -> showStatus
@18:01:01.05 -> Cmd.loadCartridge -6
@18:01:01.12 Gate.power :main,:ON,limit to 5000mA
@18:01:01.13 Gate.power :analytic1,:ON,limit to 5000mA
@18:01:01.16 Gate.power :analytic2,:ON,limit to 5000mA
@18:01:01.19 Gate.power :analytic3,:ON,limit to 5000mA
@18:01:01.32 Powering core bus
@18:01:03.64 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@18:01:04.05 <LRAUV> Archive.configure :default
@18:01:04.18 Air.configure :default
@18:01:04.29 Lysis1.configure :default
@18:01:04.39 Lysis2.configure :default
@18:01:04.52 Plunge.configure :default
@18:01:04.64 SamplePump.configure :default
@18:01:04.77 Toroid.configure :default
@18:01:04.90 Carousel.configure :default
@18:01:05.02 Pressure.configure :default
@18:01:05.20 ExtraPump.configure :default
@18:01:05.41 PinPort.configure :default
@18:01:05.55 All elf controllers running firmware version 4.79
@18:01:05.62 Gate.power :motor,:ON,limit to 5000mA
@18:01:06.70 <Toroid_home> Toroid.in :default
@18:01:06.74 <Plunge_home> Plunge.in :default
@18:01:06.78 <Lysis1_home> Lysis1.in :default
@18:01:06.81 <Archive_home> Archive.in :default
@18:01:06.85 <Lysis2_home> Lysis2.in :default
@18:01:06.89 <PinPort_home> PinPort.in :default
@18:01:06.95 <Air_home> Air.in :default
@18:01:07.11 <Toroid_home> Toroid.home.jog 0
@18:01:07.18 <Plunge_home> Plunge.home.jog 0
@18:01:07.22 <Archive_home> Archive.home.jog 0
@18:01:07.25 <PinPort_home> PinPort.home.jog 0
@18:01:07.27 <Lysis1_home> Lysis1.home.jog 0
@18:01:07.31 <Lysis2_home> Lysis2.home.jog 0
@18:01:07.35 <Air_home> Air.home.jog 0
@18:01:07.89 <Plunge_home> Plunge.in :homing
@18:01:07.98 <PinPort_home> PinPort.in :homing
@18:01:08.03 <Archive_home> Archive.in :homing
@18:01:08.06 <Toroid_home> Toroid.in :homing
@18:01:08.26 Toroid.jog 646
@18:01:08.29 <Archive_home> Archive.jog 1390
@18:01:08.31 <PinPort_home> PinPort.jog -107
@18:01:08.38 <Plunge_home> Plunge.jog 1558
@18:01:08.58 <Air_home> Air.in :homing
@18:01:08.61 <Lysis2_home> Lysis2.in :homing
@18:01:08.64 <Lysis1_home> Lysis1.in :homing
@18:01:08.80 <Air_home> Air.jog 152
@18:01:08.85 <Lysis1_home> Lysis1.jog 1383
@18:01:08.88 <Lysis2_home> Lysis2.jog 1395
@18:01:09.03 <PinPort_home> PinPort.setRawPosition! 0
@18:01:09.09 PinPort.seek :home
@18:01:09.17 PinPort.in :default
@18:01:09.32 <Air_home> Air.setRawPosition! 50
@18:01:09.37 Air.seek :home
@18:01:09.56 <Archive_home> Archive.setRawPosition! 50
@18:01:09.61 Archive.seek :home
@18:01:09.72 <Air_home> Air.in :default
@18:01:09.75 <Plunge_home> Plunge.setRawPosition! 50
@18:01:09.84 Plunge.seek :home
@18:01:09.93 <Archive_home> Archive.in :default
@18:01:10.07 <Lysis1_home> Lysis1.setRawPosition! 50
@18:01:10.15 Lysis1.seek :home
@18:01:10.24 <Plunge_home> Plunge.in :default
@18:01:10.27 <Lysis2_home> Lysis2.setRawPosition! 50
@18:01:10.36 Lysis2.seek :home
@18:01:10.47 <Lysis1_home> Lysis1.in :default
@18:01:10.57 <Toroid_home> Toroid.setRawPosition! 0
@18:01:10.61 Toroid.seek :home
@18:01:10.72 Toroid.in :default
@18:01:10.75 <Lysis2_home> Lysis2.in :default
@18:01:11.18 <LRAUV> Toroid.seek :clear
@18:01:12.80 Carousel.in :default
@18:01:12.86 Carousel.home.jog 0
@18:01:13.34 Carousel.in :default
@18:01:13.41 Carousel.seek 39
@18:01:13.57 Selecting Cartridge 38
@18:01:13.84 Carousel.seek 38
@18:01:14.33 Lysis1.in :fast
@18:01:14.39 Lysis1.seek :locked
@18:01:17.01 <Plunge> Plunge.seek :locked
@18:01:17.14 <Air> Air.in :fast
@18:01:17.20 Air.seek :locked
@18:01:19.68 <LRAUV> Powering cartridge bus
@18:01:22.28 Gate.setIR 1750,5000,0,6
@18:01:22.40 Gate.setIR 0,5000,0,6
@18:01:22.45 Gate.power :cartridge,:OFF
@18:01:22.99 Mounted archive Cartridge
@18:01:40.64 -> Cmd.startFiltering
92.07@18:01:41.27 <FILTERING> Preparing to filter cartridge 38
@18:01:41.55 Priming sample loop w/100ml, bypass w/10ml
@18:01:41.56 SamplePump.setPosition! 0ml
@18:01:41.61 Bypass.open
@18:01:43.07 Intake.open
@18:01:43.79 Sea 14.7m deep (36.4psia)
@18:01:43.81 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@18:01:46.37 Vacuum tare is 54% of full scale
@18:01:46.51 <closeIntake> Intake.close
@18:01:46.57 <closeBypass> Bypass.close
@18:01:47.27 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 36.4psia
@18:01:47.30 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@18:01:51.87 Bypass.open
@18:01:52.53 Intake.open
@18:01:53.91 Pressure.in :default
@18:01:53.97 Pressure.hold
@18:01:54.15 SamplePump.in :prime
@18:01:54.21 SamplePump.seek 10ml,59 seconds
@18:02:02.84 Pressure.stop
@18:02:02.89 SamplePump.setPosition! 0ml
@18:02:02.95 Exhaust.open
@18:02:04.42 <closeBypass> Bypass.close
@18:02:05.93 <FILTERING> Pressure.in :default
@18:02:05.99 Pressure.hold
@18:02:06.15 Sea 14.5m deep (36.2psia)
@18:02:06.29 SamplePump.in :prime
@18:02:06.36 SamplePump.seek 100ml,5:16
@18:03:40.73 Pressure.stop
@18:03:40.79 Bypass.open
@18:03:41.47 Intake.close
@18:03:42.20 Toroid.in :hold
@18:03:42.26 Toroid.seek :crack
@18:03:43.77 Toroid.seek :cartridge
@18:03:44.86 Toroid.in :default
@18:03:44.92 Toroid.seek :clear
@18:03:46.88 <closeExhaust> Exhaust.close
@18:03:46.93 <closeBypass> Bypass.close
@18:03:47.65 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 36.1psia
@18:03:47.67 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@18:03:52.29 Sampling 1000.0ml
@18:03:52.31 SamplePump.setPosition! 0ml
@18:03:52.37 Exhaust.open
@18:03:53.10 Intake.open
@18:03:53.90 Pressure.in :default
@18:03:53.96 Pressure.hold
@18:03:54.08 Sea 14.4m deep (35.9psia)
@18:03:54.22 SamplePump.in :sample
@18:03:54.28 SamplePump.seek 1000ml,1:23:48
@18:03:56.94 <sampling> Cartridge 38 filtering 1/1000ml at 0.42ml/s with 26.3psi vacuum 11.1m deep (31.1psia)
@18:04:56.94 Cartridge 38 filtering 26/1000ml at 0.42ml/s with 25.1psi vacuum 12.5m deep (33.1psia)
@18:05:56.94 Cartridge 38 filtering 49/1000ml at 0.38ml/s with 25.0psi vacuum 13.1m deep (34.1psia)
@18:06:56.94 Cartridge 38 filtering 70/1000ml at 0.33ml/s with 23.9psi vacuum 13.3m deep (34.3psia)
@18:07:56.94 Cartridge 38 filtering 90/1000ml at 0.33ml/s with 26.3psi vacuum 11.5m deep (31.7psia)
@18:08:56.93 Cartridge 38 filtering 109/1000ml at 0.32ml/s with 26.1psi vacuum 10.8m deep (30.7psia)
@18:09:56.94 Cartridge 38 filtering 127/1000ml at 0.30ml/s with 23.9psi vacuum 11.4m deep (31.6psia)
@18:10:56.93 Cartridge 38 filtering 144/1000ml at 0.25ml/s with 22.8psi vacuum 11.5m deep (31.7psia)
@18:11:56.94 Cartridge 38 filtering 160/1000ml at 0.25ml/s with 26.3psi vacuum 11.5m deep (31.7psia)
@18:12:56.93 Cartridge 38 filtering 176/1000ml at 0.25ml/s with 23.4psi vacuum 12.0m deep (32.5psia)
@18:13:56.94 Cartridge 38 filtering 191/1000ml at 0.25ml/s with 22.4psi vacuum 11.2m deep (31.2psia)
@18:14:56.93 Cartridge 38 filtering 205/1000ml at 0.25ml/s with 22.6psi vacuum 13.0m deep (34.0psia)
@18:15:56.94 Cartridge 38 filtering 218/1000ml at 0.24ml/s with 23.3psi vacuum 12.2m deep (32.8psia)
@18:16:56.93 Cartridge 38 filtering 231/1000ml at 0.21ml/s with 24.7psi vacuum 11.8m deep (32.2psia)
@18:17:56.94 Cartridge 38 filtering 244/1000ml at 0.20ml/s with 24.9psi vacuum 10.9m deep (30.9psia)
@18:18:56.94 Cartridge 38 filtering 256/1000ml at 0.20ml/s with 25.8psi vacuum 10.6m deep (30.4psia)
@18:19:16.18 <FILTERING> Flow averaged 0.28ml/s over 15:22
@18:19:16.21 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi
@18:19:16.81 Relieved excess vacuum in .6 seconds
@18:19:16.84 SamplePump.seek 1000ml,1:02:09
@18:19:51.18 Flow averaged 0.20ml/s over 35 seconds
@18:19:51.21 Waiting up to 12 seconds for vacuum to reduce from 27.4 to 18.0psi
@18:19:51.79 Relieved excess vacuum in .6 seconds
@18:19:51.82 SamplePump.seek 1000ml,1:01:35
@18:19:56.93 <sampling> Cartridge 38 filtering 268/1000ml at 0.20ml/s with 27.8psi vacuum 10.2m deep (29.8psia)
@18:20:01.81 <FILTERING> Flow averaged 0.20ml/s over 10 seconds
@18:20:01.83 <closeIntake> Intake.close
@18:20:01.91 <closeExhaust> Exhaust.close
@18:20:02.08 <FILTERING> Toroid.in :hold
@18:20:02.14 Toroid.seek :bypass
@18:20:03.76 Toroid.in :default
@18:20:03.83 Toroid.seek :clear
@18:20:05.44 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@18:20:05.46 Pressure.stop
@18:20:05.65 Sampled  268.8ml
@18:20:07.64 <LRAUV> -> Cmd.startProcessing
@18:20:08.03 <PROCESSING> Plunge.seek :reagent
@18:20:08.84 Sample loop closed. Ready to Deliver Reagents
@18:20:08.85 Archive.seek :sealed
@18:20:12.79 Archive.seek :evac
@18:20:26.83 delay 1:00
@18:21:26.85 Archive.seek :clear
@18:21:44.35 RNAlater soak for 10:00
@18:31:44.38 Archive.seek :tVent
@18:31:46.82 delay 1 second
@18:31:47.84 Archive.seek :evac
@18:32:03.35 delay 10 seconds
@18:32:13.37 Sample preserved and evacuated
@18:32:14.47 <LRAUV> -> Cmd.stop
@18:32:14.62 Carousel.seek 38
@18:32:14.80 Plunge.seek :locked
@18:32:16.12 <Archive> Archive.in :unlocking
@18:32:16.25 Archive.seek :clear
@18:32:16.29 <Air> Air.in :unlocking
@18:32:16.45 Air.seek :clear
@18:32:16.49 <Lysis1> Lysis1.in :unlocking
@18:32:16.66 Lysis1.seek :clear
@18:32:20.41 <LRAUV> Gate.power :motor,:OFF
@18:32:20.42 Gate.power :core,:OFF
@18:32:20.45 Gate.power :analytic1,:OFF
@18:32:20.48 Gate.power :analytic2,:OFF
@18:32:20.50 Gate.power :analytic3,:OFF
@18:32:20.52 Gate.power :main,:OFF
@18:32:20.54 Safely stopped and ready to power off
##-- file closed 2022-05-29T01:44:06.215Z

##-- file opened 2022-05-29T01:44:06.217Z
@18:44:07.29 -> showStatus
@18:44:09.31 -> Cmd.loadCartridge -10
@18:44:09.38 Gate.power :main,:ON,limit to 5000mA
@18:44:09.39 Gate.power :analytic1,:ON,limit to 5000mA
@18:44:09.42 Gate.power :analytic2,:ON,limit to 5000mA
@18:44:09.45 Gate.power :analytic3,:ON,limit to 5000mA
@18:44:09.60 Powering core bus
@18:44:11.85 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@18:44:12.37 <LRAUV> Archive.configure :default
@18:44:12.63 Air.configure :default
@18:44:12.84 Lysis1.configure :default
@18:44:13.09 Lysis2.configure :default
@18:44:13.41 Plunge.configure :default
@18:44:13.76 SamplePump.configure :default
@18:44:13.92 Toroid.configure :default
@18:44:14.05 Carousel.configure :default
@18:44:14.18 Pressure.configure :default
@18:44:14.30 ExtraPump.configure :default
@18:44:14.41 PinPort.configure :default
@18:44:14.47 All elf controllers running firmware version 4.79
@18:44:14.49 Gate.power :motor,:ON,limit to 5000mA
@18:44:14.62 <Archive_home> Archive.in :default
@18:44:14.98 Archive.home.jog 0
@18:44:15.13 <Toroid_home> Toroid.in :default
@18:44:15.20 <Lysis2_home> Lysis2.in :default
@18:44:15.23 <Air_home> Air.in :default
@18:44:15.27 <PinPort_home> PinPort.in :default
@18:44:15.30 <Plunge_home> Plunge.in :default
@18:44:15.40 <Lysis1_home> Lysis1.in :default
@18:44:15.61 <Toroid_home> Toroid.home.jog 0
@18:44:15.66 <Air_home> Air.home.jog 0
@18:44:15.68 <Lysis2_home> Lysis2.home.jog 0
@18:44:15.71 <Plunge_home> Plunge.home.jog 0
@18:44:15.73 <PinPort_home> PinPort.home.jog 0
@18:44:15.76 <Lysis1_home> Lysis1.home.jog 0
@18:44:16.20 <Archive_home> Archive.in :homing
@18:44:16.37 Archive.jog 1391
@18:44:16.50 <Toroid_home> Toroid.in :homing
@18:44:16.57 <PinPort_home> PinPort.in :homing
@18:44:16.61 <Plunge_home> Plunge.in :homing
@18:44:16.65 <Lysis2_home> Lysis2.in :homing
@18:44:16.69 <Lysis1_home> Lysis1.in :homing
@18:44:16.72 <Air_home> Air.in :homing
@18:44:16.88 <Toroid_home> Toroid.jog 641
@18:44:16.97 <PinPort_home> PinPort.jog -107
@18:44:17.01 <Lysis2_home> Lysis2.jog 1393
@18:44:17.05 <Lysis1_home> Lysis1.jog 1382
@18:44:17.08 <Plunge_home> Plunge.jog 1552
@18:44:17.12 <Air_home> Air.jog 1385
@18:44:17.35 <PinPort_home> PinPort.setRawPosition! 0
@18:44:17.40 PinPort.seek :home
@18:44:17.50 PinPort.in :default
@18:44:17.58 <Archive_home> Archive.setRawPosition! 50
@18:44:17.63 Archive.seek :home
@18:44:17.91 Archive.in :default
@18:44:18.31 <Lysis2_home> Lysis2.setRawPosition! 50
@18:44:18.37 Lysis2.seek :home
@18:44:18.47 <Plunge_home> Plunge.setRawPosition! 50
@18:44:18.50 <Lysis1_home> Lysis1.setRawPosition! 50
@18:44:18.52 <Air_home> Air.setRawPosition! 50
@18:44:18.63 <Plunge_home> Plunge.seek :home
@18:44:18.67 <Lysis1_home> Lysis1.seek :home
@18:44:18.74 <Air_home> Air.seek :home
@18:44:18.90 <Lysis2_home> Lysis2.in :default
@18:44:18.99 <Plunge_home> Plunge.in :default
@18:44:19.11 <Lysis1_home> Lysis1.in :default
@18:44:19.13 <Air_home> Air.in :default
@18:44:19.26 <Toroid_home> Toroid.setRawPosition! 0
@18:44:19.31 Toroid.seek :home
@18:44:19.41 Toroid.in :default
@18:44:19.55 <LRAUV> Toroid.seek :clear
@18:44:21.17 Carousel.in :default
@18:44:21.23 Carousel.home.jog 0
@18:44:21.72 Carousel.in :default
@18:44:21.78 Carousel.seek 38
@18:44:21.95 Selecting Cartridge 37
@18:44:22.21 Carousel.seek 37
@18:44:22.70 Lysis1.in :fast
@18:44:22.76 Lysis1.seek :locked
@18:44:25.36 <Plunge> Plunge.seek :locked
@18:44:25.49 <Air> Air.in :fast
@18:44:25.54 Air.seek :locked
@18:44:28.00 <LRAUV> Powering cartridge bus
@18:44:30.60 Gate.setIR 1750,5000,0,6
@18:44:30.73 Gate.setIR 0,5000,0,6
@18:44:30.79 PuckHeater.configure :default
@18:44:30.85 Gate.power :heat,:ON,limit to 5000mA
@18:44:31.83 Mounted lysis Cartridge board#Q05032
@18:44:31.86 Using default puck heat loss curve for HeatingAccelInCarousel
@18:44:49.71 -> Cmd.startFiltering
@18:44:49.96 <FILTERING> Preparing to filter cartridge 37
@18:44:50.25 Priming sample loop w/100ml, bypass w/10ml
@18:44:50.26 SamplePump.setPosition! 0ml
@18:44:50.31 Bypass.open
@18:44:51.78 Intake.open
@18:44:52.53 Sea 15.3m deep (37.2psia)
@18:44:52.55 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@18:44:55.13 Vacuum tare is 54% of full scale
@18:44:55.26 <closeIntake> Intake.close
@18:44:55.32 <closeBypass> Bypass.close
@18:44:56.07 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 37.2psia
@18:44:56.09 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@18:45:00.66 Bypass.open
@18:45:01.33 Intake.open
@18:45:02.74 Pressure.in :default
@18:45:02.80 Pressure.hold
@18:45:02.98 SamplePump.in :prime
@18:45:03.05 SamplePump.seek 10ml,59 seconds
@18:45:11.68 Pressure.stop
@18:45:11.73 SamplePump.setPosition! 0ml
@18:45:11.78 Exhaust.open
@18:45:13.25 <closeBypass> Bypass.close
@18:45:14.71 <FILTERING> Pressure.in :default
@18:45:14.77 Pressure.hold
@18:45:14.89 Sea 15.4m deep (37.5psia)
@18:45:15.04 SamplePump.in :prime
@18:45:15.11 SamplePump.seek 100ml,5:16
@18:46:49.08 Pressure.stop
@18:46:49.14 Bypass.open
@18:46:49.82 Intake.close
@18:46:50.54 Toroid.in :hold
@18:46:50.60 Toroid.seek :crack
@18:46:52.10 Toroid.seek :cartridge
@18:46:53.20 Toroid.in :default
@18:46:53.26 Toroid.seek :clear
@18:46:55.22 <closeExhaust> Exhaust.close
@18:46:55.24 <closeBypass> Bypass.close
@18:46:56.04 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 39.9psia
@18:46:56.05 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@18:47:00.68 Sampling 1000.0ml
@18:47:00.69 SamplePump.setPosition! 0ml
@18:47:00.75 Exhaust.open
@18:47:01.51 Intake.open
@18:47:02.31 Pressure.in :default
@18:47:02.37 Pressure.hold
@18:47:02.49 Sea 16.9m deep (39.7psia)
@18:47:02.63 SamplePump.in :sample
@18:47:02.75 SamplePump.seek 1000ml,1:23:48
@18:47:05.35 <sampling> Cartridge 37 filtering 1/1000ml at 0.41ml/s with 27.5psi vacuum 16.2m deep (38.6psia)
@18:48:05.35 Cartridge 37 filtering 26/1000ml at 0.42ml/s with 25.7psi vacuum 17.1m deep (40.0psia)
@18:49:05.35 Cartridge 37 filtering 50/1000ml at 0.36ml/s with 25.0psi vacuum 16.7m deep (39.4psia)
@18:50:05.34 Cartridge 37 filtering 72/1000ml at 0.37ml/s with 25.5psi vacuum 17.8m deep (41.0psia)
@18:51:05.35 Cartridge 37 filtering 92/1000ml at 0.35ml/s with 25.7psi vacuum 16.6m deep (39.2psia)
@18:52:05.35 Cartridge 37 filtering 112/1000ml at 0.33ml/s with 21.3psi vacuum 17.2m deep (40.2psia)
@18:53:05.35 Cartridge 37 filtering 131/1000ml at 0.32ml/s with 21.4psi vacuum 17.4m deep (40.4psia)
@18:54:05.34 Cartridge 37 filtering 149/1000ml at 0.26ml/s with 22.7psi vacuum 18.3m deep (41.8psia)
@18:55:05.35 Cartridge 37 filtering 166/1000ml at 0.26ml/s with 23.6psi vacuum 16.7m deep (39.4psia)
@18:56:05.34 Cartridge 37 filtering 182/1000ml at 0.26ml/s with 25.3psi vacuum 17.7m deep (40.8psia)
@18:57:05.35 Cartridge 37 filtering 198/1000ml at 0.27ml/s with 22.1psi vacuum 18.2m deep (41.6psia)
@18:58:05.34 Cartridge 37 filtering 213/1000ml at 0.23ml/s with 23.7psi vacuum 16.9m deep (39.7psia)
@18:59:05.35 Cartridge 37 filtering 227/1000ml at 0.23ml/s with 25.3psi vacuum 16.5m deep (39.1psia)
@19:00:05.34 Cartridge 37 filtering 241/1000ml at 0.22ml/s with 23.1psi vacuum 19.8m deep (44.0psia)
@19:01:05.35 Cartridge 37 filtering 254/1000ml at 0.23ml/s with 22.5psi vacuum 18.7m deep (42.4psia)
@19:02:05.34 Cartridge 37 filtering 267/1000ml at 0.21ml/s with 23.4psi vacuum 17.6m deep (40.7psia)
@19:03:05.35 Cartridge 37 filtering 279/1000ml at 0.20ml/s with 24.0psi vacuum 17.5m deep (40.5psia)
@19:04:05.34 Cartridge 37 filtering 292/1000ml at 0.21ml/s with 22.9psi vacuum 17.2m deep (40.1psia)
@19:04:35.67 <FILTERING> Flow averaged 0.28ml/s over 17:34
@19:04:35.71 Waiting up to 14 seconds for vacuum to reduce from 28.5 to 18.0psi
@19:04:36.29 Relieved excess vacuum in .6 seconds
@19:04:36.31 SamplePump.seek 1000ml,59:01
@19:05:05.34 <sampling> Cartridge 37 filtering 303/1000ml at 0.20ml/s with 26.5psi vacuum 15.5m deep (37.6psia)
@19:05:07.45 <FILTERING> Flow averaged 0.20ml/s over 32 seconds
@19:05:07.48 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi
@19:05:08.06 Relieved excess vacuum in .6 seconds
@19:05:08.08 SamplePump.seek 1000ml,58:30
@19:05:37.60 Flow averaged 0.20ml/s over 30 seconds
@19:05:37.63 Waiting up to 12 seconds for vacuum to reduce from 27.7 to 18.0psi
@19:05:38.21 Relieved excess vacuum in .6 seconds
@19:05:38.23 SamplePump.seek 1000ml,58:00
@19:05:40.09 Flow estimate reduced to 0.16ml/s
@19:05:40.11 <closeIntake> Intake.close
@19:05:40.19 <closeExhaust> Exhaust.close
@19:05:40.36 <FILTERING> Toroid.in :hold
@19:05:40.43 Toroid.seek :bypass
@19:05:42.05 Toroid.in :default
@19:05:42.11 Toroid.seek :clear
@19:05:43.72 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@19:05:43.74 Pressure.stop
@19:05:43.93 Sampled  310.1ml
@19:05:46.19 <LRAUV> -> Cmd.startProcessing
@19:05:47.63 <PROCESSING> SPR.command \"read_sequences\"
@19:05:47.67 SPR.command \"stats_on\"
@19:05:48.52 SPR.command \"start 0 1\"
@19:05:49.26 <SPRlogger> SPR.start /var/log/esp/spr22may2819h.spr.bz2
@19:05:52.25 <PROCESSING> Handoff.select :waste
@19:05:58.92 Syringe tare is 18% of full scale
@19:05:58.94 PinPort.in :locking
@19:05:59.02 PinPort.seek :locked
@19:05:59.50 <posArchive> Archive.in :fast
@19:05:59.56 <posLyS2> Lysis2.in :fast
@19:05:59.60 <PROCESSING> Lysis1.seek 1350uL
@19:05:59.74 <posArchive> Archive.seek :full
@19:05:59.78 <posLyS2> Lysis2.seek :full
@19:06:01.74 <PROCESSING> Plunge.seek :reagent
@19:06:02.61 Evacuating puck with filter bubble point of 50psi
@19:06:02.65 PinPort.seek :locked
@19:06:02.74 Handoff.select :waste
@19:06:09.32 Air syringe pumping up to 1 stroke to reach 8psi
@19:06:09.33 Air stroke #1 of 1
@19:06:09.42 Air.in :pressurize
@19:06:09.48 Air.seek :pressurized
@19:06:21.08 -0.0psi after pressurize
@19:06:21.12 Air.in :compress
@19:06:21.19 Air.seek :evac
@19:06:25.29 0.7psi after compress
@19:06:37.86 0.1psi after compress (stable)
@19:06:37.87 Air.in :default
@19:06:37.94 Air.hold
@19:06:38.14 at 0.1psi Opened to waste
@19:06:38.15 Handoff.select :blocked
@19:06:38.29 Archive syringe pumping up to 8 strokes to reach 50psi
@19:06:38.30 Archive stroke #1 of 8
@19:06:38.39 Archive.in :pressurize
@19:06:38.45 Archive.seek :pressurized
@19:06:51.33 10.0psi after pressurize
@19:06:51.36 Archive.in :compress
@19:06:51.45 Archive.seek :empty
@19:07:06.14 30.6psi after compress
@19:07:25.72 40.2psi after compress (stable)
@19:07:25.74 Archive.in :gulp
@19:07:25.82 Archive.seek :topVent
@19:07:33.00 41.4psi after gulp
@19:07:45.57 41.2psi after gulp (stable)
@19:07:45.58 Archive stroke #2 of 8
@19:07:45.68 Archive.in :pressurize
@19:07:45.74 Archive.seek :pressurized
@19:07:58.60 40.1psi after pressurize
@19:07:58.62 Archive.in :compress
@19:07:58.70 Archive.seek :empty
@19:08:11.45 Reached 50.3 of 50.0psi after pumping 3418uL (1.98 strokes)
@19:08:11.47 Archive.in :default
@19:08:11.54 Archive.hold
@19:08:11.60 Handoff.select :waste
@19:08:21.79 delay 1:00
@19:09:21.81 Handoff.select :blocked
@19:09:53.07 Pressure too High, open to waste again (8.0psi)
@19:09:53.08 Handoff.select :waste
@19:09:53.21 delay 1:00
@19:10:53.22 Handoff.select :blocked
@19:11:21.41 Handoff.select :waste
@19:11:21.52 4.0psi at evacEnd
@19:11:21.53 Handoff.select :blocked
@19:11:21.67 Archive.in :fast
@19:11:21.73 Archive.seek :topVent
@19:11:35.24 Stable @3.0psi after Handoff blocked
@19:11:35.26 Archive syringe pumping up to 8 strokes to reach 33psi
@19:11:35.28 Archive stroke #1 of 8
@19:11:35.38 Archive.in :pressurize
@19:11:35.44 Archive.seek :pressurized
@19:11:48.32 4.4psi after pressurize
@19:11:48.35 Archive.in :compress
@19:11:48.45 Archive.seek :empty
@19:12:03.14 7.7psi after compress
@19:12:44.72 20.0psi after compress (stable)
@19:12:44.73 Archive.in :gulp
@19:12:44.81 Archive.seek :topVent
@19:12:51.97 21.1psi after gulp
@19:13:04.55 21.7psi after gulp (stable)
@19:13:04.56 Archive stroke #2 of 8
@19:13:04.65 Archive.in :pressurize
@19:13:04.72 Archive.seek :pressurized
@19:13:17.59 22.3psi after pressurize
@19:13:17.61 Archive.in :compress
@19:13:17.69 Archive.seek :empty
@19:13:32.41 24.9psi after compress
@19:13:52.03 Reached 33.0 of 33.0psi after pumping 3460uL (2.00 strokes)
@19:13:52.04 Archive.in :default
@19:13:52.12 Archive.hold
@19:13:52.18 Handoff.select :waste
@19:13:59.36 delay 1:00
@19:14:59.39 Handoff.select :blocked
@19:15:22.11 Handoff.select :waste
@19:15:22.24 2.6psi at evacEnd
@19:15:22.25 Air.seek :fullVent
@19:15:23.69 Archive.in :fast
@19:15:23.75 Archive.seek :topVent
@19:15:30.86 Seawater evacuated, Presure above/below filter released. Lysis begins
@19:15:30.95 Lysis1.in :slow
@19:15:31.01 Lysis1.seek 1050uL
@19:16:01.06 delay 5 seconds
@19:16:06.07 Handoff.select :blocked
@19:16:06.25 Lysis1.seek 450uL
@19:17:06.25 delay 3 seconds
@19:17:09.32 Air.in mix with 1.40048 parts Lysis1
@19:17:09.80 <Airmixer> Air.seek :evac
@19:17:09.86 <PROCESSING> Lysis1.seek 300uL
@19:17:25.00 Lysis1.seek :empty
@19:17:55.13 Lysis buffer delivered @29.6psi
@19:17:55.15 Polling Can every 2:00
@19:17:55.69 PuckHeater.configure :default #ambient 14.4C
@19:17:55.74 PuckHeater.seek 80.1C
@19:19:06.09 PuckHeater.hold 2:00,80.1C
@19:21:05.98 Polling Can every 2:00
@19:21:06.52 PuckHeater.hold 30 seconds,79.1C
@19:21:36.66 Polling Can every 2:00
@19:21:37.22 PuckHeater.configure :default #ambient 14.4C
@19:21:37.27 PuckHeater.hold 30 seconds,78.5C
@19:22:07.40 Polling Can every 2:00
@19:22:07.94 PuckHeater.configure :default #ambient 14.4C
@19:22:08.00 PuckHeater.hold 10:00,78C
@19:32:07.05 Lysis complete. Syringe pressure @40.4psi
@19:32:07.06 Handoff.select :blocked
@19:32:07.11 Archive syringe pumping up to 5 strokes to reach 40psi
@19:32:07.12 Archive stroke #1 of 5
@19:32:07.22 Archive.in :pressurize
@19:32:07.28 Archive.seek :pressurized
@19:32:07.45 Reached 40.4 of 40.0psi after pumping 0uL (0.00 strokes)
@19:32:07.46 Archive.in :default
@19:32:07.56 Archive.hold
@19:32:07.64 delay 2 seconds
@19:32:09.66 Archive.in :fast
@19:32:09.72 Archive.seek :full
@19:32:09.88 Lysis1.seek 138uL
@19:32:23.77 delay 1 second
@19:32:24.79 <fillArchive> Archive.seek :full
@19:32:24.83 <PROCESSING> Lysis1.seek :empty
@19:32:38.82 Initial air pressure @40.0psi
@19:32:38.89 Lysis1.in :extract
@19:32:38.96 Lysis1.seek 600uL,nil
@19:32:40.70 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@19:32:40.71 Archive stroke #1 of 6
@19:32:40.81 Archive.in :pressurize
@19:32:40.87 Archive.seek :pressurized
@19:32:53.76 15.9psi after pressurize
@19:32:53.79 Archive.in :compress
@19:32:53.88 Archive.seek :empty
@19:33:06.54 Syringe::LysatePlungerStuck in PROCESSING -- at < 401uL during Lysis Syringe #1 (LyS1) move
@19:33:06.57 <PROCESSING> Lysis1.in :default
@19:33:06.64 Lysis1.seek 366uL
@19:33:07.35 <pump> Reached 30.2 of 25.0psi after pumping 1704uL (0.99 strokes)
@19:33:07.37 Archive.in :default
@19:33:07.44 Archive.hold
@19:33:08.46 <PROCESSING> Lysis1.in :extract
@19:33:08.52 Lysis1.seek 600uL,nil
@19:33:12.09 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@19:33:12.10 Archive stroke #1 of 6
@19:33:12.19 Archive.in :compress
@19:33:12.26 Archive.seek :empty
@19:33:13.09 Archive.in :default
@19:33:13.14 Archive.hold
@19:33:13.29 <PROCESSING> Initial 600uL lysate @17.5psi
@19:33:13.31 Handoff.select :blocked
@19:33:13.41 Initial air pressure @18.3psi
@19:33:13.49 Lysis1.seek 825uL,nil
@19:33:14.34 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@19:33:14.35 Archive stroke #1 of 6
@19:33:14.51 Archive.in :compress
@19:33:14.57 Archive.seek :empty
@19:33:15.18 16.8psi after compress
@19:33:27.06 Archive.in :default
@19:33:27.12 Archive.hold
@19:33:27.30 <PROCESSING> Recovered 825uL lysate @16.2psi
@19:33:27.32 Air.in :halfSpeed
@19:33:27.39 Air.seek :fullVent
@19:33:29.61 Puck Top Vented, bottom @1.4psi
@19:33:29.62 Handoff.select :waste
@19:33:29.75 delay 5 seconds
@19:33:34.82 Handoff open to waste, bottom @0.0psi
@19:33:34.84 Air.in :default
@19:33:34.91 Air.seek :evac
@19:33:36.32 Lysis2.in :DAmix
@19:33:36.45 Lysis1.in :DAmix
@19:33:36.93 <Lysis2mixer> Lysis2.seek 1480uL
@19:33:36.97 <PROCESSING> Lysis1.seek 575uL
@19:34:12.03 delay 5 seconds
@19:34:17.05 Handoff.select SPR
@19:34:17.20 SPR.command \"start 2 14\"
@19:34:19.02 SPR stabilized
@19:34:19.15 delay 10 seconds
@19:34:29.16 SPR.command \"start 1 0\"
@19:34:31.89 Waiting up to 4:00 for SPR to enter state 1
@19:36:34.27 SPR.command \"hold_on\"
@19:36:35.68 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@19:36:36.15 <Lysis2mixer> Lysis2.seek 1180uL
@19:36:36.22 <PROCESSING> Lysis1.seek 275uL
@19:37:18.25 delay 15 seconds
@19:37:33.27 SPR.command \"hold_off\"
@19:37:36.36 Waiting up to 38:00 for SPR to enter state 7
@19:58:26.16 <SPRlogger> SPRsummary:2.04133,137.263,128.524,137.9 after 3310s
@20:05:03.38 <PROCESSING> SPR analysis completed
@20:05:09.59 <SPRlogger> Ended log spr22may2819h.spr.bz2
@20:05:09.60 <PROCESSING> SPR.command \"stats_off\"
@20:05:11.23 <LRAUV> -> Cmd.stop
@20:05:11.32 <closeHandoff> Handoff.select :close
@20:05:11.46 <LRAUV> Gate.power :heat,:OFF
@20:05:11.47 Gate.power :cartridge,:OFF
@20:05:11.56 Carousel.seek 37
@20:05:11.74 Plunge.seek :locked
@20:05:12.61 PinPort.in :unlocking
@20:05:12.67 PinPort.seek :clear
@20:05:13.21 <Archive> Archive.in :unlocking
@20:05:13.34 Archive.seek :clear
@20:05:13.43 <Air> Air.in :unlocking
@20:05:13.54 Air.seek :clear
@20:05:13.58 <Lysis1> Lysis1.in :unlocking
@20:05:13.75 <Lysis2> Lysis2.in :unlocking
@20:05:13.79 <Lysis1> Lysis1.seek :clear
@20:05:13.89 <Lysis2> Lysis2.seek :clear
@20:05:17.49 <LRAUV> Gate.power :motor,:OFF
@20:05:17.51 Gate.power :core,:OFF
@20:05:17.53 Gate.power :analytic1,:OFF
@20:05:17.56 Gate.power :analytic2,:OFF
@20:05:17.58 Gate.power :analytic3,:OFF
@20:05:17.60 Gate.power :main,:OFF
@20:05:17.62 Safely stopped and ready to power off
##-- file closed 2022-05-30T00:59:53.019Z