##-- file opened 2022-05-22T02:20:25.275Z
@19:20:25.17 -> showStatus
@19:20:27.19 -> Cmd.loadCartridge -10
@19:20:27.26 Gate.power :main,:ON,limit to 5000mA
@19:20:27.27 Gate.power :analytic1,:ON,limit to 5000mA
@19:20:27.30 Gate.power :analytic2,:ON,limit to 5000mA
@19:20:27.31 Gate.power :analytic3,:ON,limit to 5000mA
@19:20:27.46 Powering core bus
@19:20:29.71 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@19:20:30.23 <LRAUV> Archive.configure :default
@19:20:30.36 Air.configure :default
@19:20:30.46 Lysis1.configure :default
@19:20:30.56 Lysis2.configure :default
@19:20:30.69 Plunge.configure :default
@19:20:30.81 SamplePump.configure :default
@19:20:30.94 Toroid.configure :default
@19:20:31.07 Carousel.configure :default
@19:20:31.20 Pressure.configure :default
@19:20:31.32 ExtraPump.configure :default
@19:20:31.43 PinPort.configure :default
@19:20:31.49 All elf controllers running firmware version 4.79
@19:20:31.52 Gate.power :motor,:ON,limit to 5000mA
@19:20:32.01 <Archive_home> Archive.in :default
@19:20:32.62 Archive.home.jog 0
@19:20:33.02 <Toroid_home> Toroid.in :default
@19:20:33.11 <Plunge_home> Plunge.in :default
@19:20:33.20 <Lysis1_home> Lysis1.in :default
@19:20:33.23 <Air_home> Air.in :default
@19:20:33.26 <PinPort_home> PinPort.in :default
@19:20:33.30 <Lysis2_home> Lysis2.in :default
@19:20:33.49 <Toroid_home> Toroid.home.jog 0
@19:20:33.52 <Plunge_home> Plunge.home.jog 0
@19:20:33.54 <Lysis1_home> Lysis1.home.jog 0
@19:20:33.57 <PinPort_home> PinPort.home.jog 0
@19:20:33.60 <Air_home> Air.home.jog 0
@19:20:33.63 <Lysis2_home> Lysis2.home.jog 0
@19:20:34.03 <Archive_home> Archive.in :homing
@19:20:34.17 Archive.jog 155
@19:20:34.24 <Toroid_home> Toroid.in :homing
@19:20:34.27 <Plunge_home> Plunge.in :homing
@19:20:34.41 <PinPort_home> PinPort.in :homing
@19:20:34.43 <Lysis1_home> Lysis1.in :homing
@19:20:34.58 <Plunge_home> Plunge.jog 1565
@19:20:34.61 <Toroid_home> Toroid.jog 653
@19:20:34.70 <PinPort_home> PinPort.jog -104
@19:20:34.72 <Lysis1_home> Lysis1.jog 1382
@19:20:34.91 <Air_home> Air.in :homing
@19:20:34.92 <Archive_home> Archive.setRawPosition! 50
@19:20:34.96 <Lysis2_home> Lysis2.in :homing
@19:20:35.07 <Archive_home> Archive.seek :home
@19:20:35.11 <Lysis2_home> Lysis2.jog 1407
@19:20:35.15 <Air_home> Air.jog 152
@19:20:35.32 <PinPort_home> PinPort.setRawPosition! 0
@19:20:35.41 PinPort.seek :home
@19:20:35.52 <Archive_home> Archive.in :default
@19:20:35.54 <PinPort_home> PinPort.in :default
@19:20:35.67 <Air_home> Air.setRawPosition! 50
@19:20:35.72 Air.seek :home
@19:20:35.86 <Plunge_home> Plunge.setRawPosition! 50
@19:20:35.92 Plunge.seek :home
@19:20:36.02 <Lysis1_home> Lysis1.setRawPosition! 50
@19:20:36.04 <Air_home> Air.in :default
@19:20:36.12 <Lysis1_home> Lysis1.seek :home
@19:20:36.27 <Plunge_home> Plunge.in :default
@19:20:36.36 <Lysis2_home> Lysis2.setRawPosition! 50
@19:20:36.41 Lysis2.seek :home
@19:20:36.50 <Lysis1_home> Lysis1.in :default
@19:20:36.70 <Lysis2_home> Lysis2.in :default
@19:20:36.94 <Toroid_home> Toroid.setRawPosition! 0
@19:20:36.99 Toroid.seek :home
@19:20:37.08 Toroid.in :default
@19:20:37.21 <LRAUV> Toroid.seek :clear
@19:20:38.82 Carousel.in :default
@19:20:38.88 Carousel.home.jog 0
@19:20:39.36 Carousel.in :default
@19:20:39.42 Carousel.seek 47
@19:20:39.56 Selecting Cartridge 46
@19:20:39.82 Carousel.seek 46
@19:20:40.30 Lysis1.in :fast
@19:20:40.36 Lysis1.seek :locked
@19:20:42.97 <Plunge> Plunge.seek :locked
@19:20:43.10 <Air> Air.in :fast
@19:20:43.16 Air.seek :locked
@19:20:45.62 <LRAUV> Powering cartridge bus
@19:20:48.23 Gate.setIR 1750,5000,0,6
@19:20:48.35 Gate.setIR 0,5000,0,6
@19:20:48.41 PuckHeater.configure :default
@19:20:48.46 Gate.power :heat,:ON,limit to 5000mA
@19:20:49.12 Mounted lysis Cartridge board#Q05060
@19:20:49.15 Using default puck heat loss curve for HeatingAccelInCarousel
@19:21:07.64 -> Cmd.startFiltering
@19:21:07.87 <FILTERING> Preparing to filter cartridge 46
@19:21:08.14 Priming sample loop w/100ml, bypass w/10ml
@19:21:08.15 SamplePump.setPosition! 0ml
@19:21:08.20 Bypass.open
@19:21:09.66 Intake.open
@19:21:10.42 Sea 5.4m deep (22.6psia)
@19:21:10.44 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@19:21:13.00 Vacuum tare is 54% of full scale
@19:21:13.13 Pressure.in :default
@19:21:13.19 Pressure.hold
@19:21:13.37 SamplePump.in :prime
@19:21:13.44 SamplePump.seek 10ml,59 seconds
@19:21:22.07 Pressure.stop
@19:21:22.12 SamplePump.setPosition! 0ml
@19:21:22.17 Exhaust.open
@19:21:23.62 <closeBypass> Bypass.close
@19:21:25.12 <FILTERING> Pressure.in :default
@19:21:25.18 Pressure.hold
@19:21:25.29 Sea 5.8m deep (23.2psia)
@19:21:25.43 SamplePump.in :prime
@19:21:25.50 SamplePump.seek 100ml,5:16
@19:23:00.03 Pressure.stop
@19:23:00.09 Bypass.open
@19:23:00.75 Intake.close
@19:23:01.51 Toroid.in :hold
@19:23:01.57 Toroid.seek :crack
@19:23:03.08 Toroid.seek :cartridge
@19:23:04.17 Toroid.in :default
@19:23:04.23 Toroid.seek :clear
@19:23:06.18 Pressurizing cartridge to 26psi above 23.6psia
@19:23:06.20 SamplePump.setPosition! 0ml
@19:23:06.26 Intake.open
@19:23:06.94 Bypass.close
@19:23:07.63 Exhaust.close
@19:23:08.46 Pressure.in :default
@19:23:08.52 Pressure.hold
@19:23:08.70 SamplePump.in :charge
@19:23:08.76 SamplePump.seek 2.3ml,42 seconds
@19:23:19.06 Pressure.stop
@19:23:19.11 Intake.close
@19:23:19.86 Pressurized cartridge with 0.509ml to 26.2psi
@19:23:19.87 Isolated @46.8psia (23.2psi)
@19:23:19.88 Verifying cartridge holds at least 0.65..0.85 of 46.8psia
@19:23:19.92 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@19:23:24.56 Sampling 1000.0ml
@19:23:24.57 SamplePump.setPosition! 0ml
@19:23:24.63 Exhaust.open
@19:23:25.35 Intake.open
@19:23:26.12 Pressure.in :default
@19:23:26.19 Pressure.hold
@19:23:26.30 Sea 6.1m deep (23.7psia)
@19:23:26.43 SamplePump.in :sample
@19:23:26.50 SamplePump.seek 1000ml,1:23:48
@19:23:29.15 <sampling> Cartridge 46 filtering 1/1000ml at 0.43ml/s with 22.3psi vacuum 6.8m deep (24.8psia)
@19:24:29.15 Cartridge 46 filtering 25/1000ml at 0.37ml/s with 23.7psi vacuum 4.1m deep (20.8psia)
@19:25:29.16 Cartridge 46 filtering 48/1000ml at 0.35ml/s with 24.5psi vacuum 5.8m deep (23.3psia)
@19:26:29.15 Cartridge 46 filtering 69/1000ml at 0.33ml/s with 25.1psi vacuum 4.5m deep (21.3psia)
@19:27:29.17 Cartridge 46 filtering 89/1000ml at 0.35ml/s with 21.2psi vacuum 5.4m deep (22.7psia)
@19:28:29.15 Cartridge 46 filtering 109/1000ml at 0.34ml/s with 26.2psi vacuum 4.5m deep (21.4psia)
@19:29:29.17 Cartridge 46 filtering 128/1000ml at 0.31ml/s with 23.6psi vacuum 3.8m deep (20.3psia)
@19:30:29.15 Cartridge 46 filtering 147/1000ml at 0.29ml/s with 23.2psi vacuum 4.4m deep (21.2psia)
@19:31:29.17 Cartridge 46 filtering 165/1000ml at 0.32ml/s with 25.7psi vacuum 5.2m deep (22.3psia)
@19:32:29.15 Cartridge 46 filtering 183/1000ml at 0.30ml/s with 26.5psi vacuum 3.9m deep (20.4psia)
@19:33:29.17 Cartridge 46 filtering 200/1000ml at 0.31ml/s with 21.7psi vacuum 5.5m deep (22.8psia)
@19:34:29.16 Cartridge 46 filtering 217/1000ml at 0.26ml/s with 22.6psi vacuum 4.7m deep (21.6psia)
@19:35:29.16 Cartridge 46 filtering 234/1000ml at 0.30ml/s with 22.0psi vacuum 5.0m deep (22.1psia)
@19:36:29.16 Cartridge 46 filtering 250/1000ml at 0.27ml/s with 25.9psi vacuum 4.1m deep (20.7psia)
@19:37:29.16 Cartridge 46 filtering 266/1000ml at 0.24ml/s with 23.6psi vacuum 4.2m deep (20.9psia)
@19:38:29.15 Cartridge 46 filtering 281/1000ml at 0.25ml/s with 23.8psi vacuum 3.8m deep (20.3psia)
@19:39:29.16 Cartridge 46 filtering 297/1000ml at 0.26ml/s with 26.7psi vacuum 2.5m deep (18.4psia)
@19:40:29.15 Cartridge 46 filtering 312/1000ml at 0.23ml/s with 24.6psi vacuum 3.1m deep (19.3psia)
@19:41:29.17 Cartridge 46 filtering 326/1000ml at 0.23ml/s with 24.9psi vacuum 3.9m deep (20.4psia)
@19:42:29.15 Cartridge 46 filtering 341/1000ml at 0.24ml/s with 22.8psi vacuum 6.0m deep (23.5psia)
@19:43:29.16 Cartridge 46 filtering 355/1000ml at 0.23ml/s with 25.3psi vacuum 3.2m deep (19.4psia)
@19:44:29.16 Cartridge 46 filtering 369/1000ml at 0.22ml/s with 24.3psi vacuum 2.8m deep (18.8psia)
@19:45:29.16 Cartridge 46 filtering 382/1000ml at 0.23ml/s with 24.9psi vacuum 4.4m deep (21.3psia)
@19:46:29.16 Cartridge 46 filtering 396/1000ml at 0.25ml/s with 23.7psi vacuum 4.4m deep (21.2psia)
@19:47:29.16 Cartridge 46 filtering 409/1000ml at 0.20ml/s with 25.2psi vacuum 2.4m deep (18.2psia)
@19:48:29.16 Cartridge 46 filtering 422/1000ml at 0.24ml/s with 24.0psi vacuum 3.5m deep (19.8psia)
@19:49:29.17 Cartridge 46 filtering 435/1000ml at 0.23ml/s with 26.9psi vacuum 3.5m deep (19.8psia)
@19:50:29.15 Cartridge 46 filtering 448/1000ml at 0.21ml/s with 24.3psi vacuum 2.8m deep (18.8psia)
@19:51:29.17 Cartridge 46 filtering 460/1000ml at 0.21ml/s with 27.7psi vacuum 2.4m deep (18.3psia)
@19:52:29.16 Cartridge 46 filtering 473/1000ml at 0.20ml/s with 25.2psi vacuum 2.4m deep (18.3psia)
@19:53:29.16 Cartridge 46 filtering 485/1000ml at 0.20ml/s with 21.4psi vacuum 2.9m deep (18.9psia)
@19:54:29.16 Cartridge 46 filtering 497/1000ml at 0.20ml/s with 28.1psi vacuum 1.9m deep (17.5psia)
@19:55:29.16 Cartridge 46 filtering 510/1000ml at 0.20ml/s with 23.5psi vacuum 1.9m deep (17.5psia)
@19:56:29.16 Cartridge 46 filtering 522/1000ml at 0.20ml/s with 26.7psi vacuum 2.6m deep (18.5psia)
@19:57:29.16 Cartridge 46 filtering 534/1000ml at 0.20ml/s with 26.6psi vacuum 3.2m deep (19.4psia)
@19:58:29.15 Cartridge 46 filtering 546/1000ml at 0.20ml/s with 26.9psi vacuum 2.7m deep (18.7psia)
@19:58:37.79 <FILTERING> Flow averaged 0.26ml/s over 35:12
@19:58:37.83 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi
@19:58:38.40 Relieved excess vacuum in .6 seconds
@19:58:38.43 SamplePump.seek 1000ml,38:10
@19:59:29.15 <sampling> Cartridge 46 filtering 558/1000ml at 0.20ml/s with 24.7psi vacuum 3.2m deep (19.5psia)
@20:00:25.39 <FILTERING> Flow averaged 0.20ml/s over 1:47
@20:00:25.42 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi
@20:00:26.00 Relieved excess vacuum in .6 seconds
@20:00:26.03 SamplePump.seek 1000ml,36:22
@20:00:29.15 <sampling> Cartridge 46 filtering 570/1000ml at 0.20ml/s with 26.4psi vacuum 2.7m deep (18.7psia)
@20:01:29.17 Cartridge 46 filtering 582/1000ml at 0.20ml/s with 26.8psi vacuum 2.9m deep (19.0psia)
@20:02:01.99 <FILTERING> Flow averaged 0.20ml/s over 1:36
@20:02:02.03 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi
@20:02:02.60 Relieved excess vacuum in .6 seconds
@20:02:02.63 SamplePump.seek 1000ml,34:45
@20:02:29.16 <sampling> Cartridge 46 filtering 594/1000ml at 0.20ml/s with 23.9psi vacuum 1.6m deep (17.1psia)
@20:02:54.68 <FILTERING> Flow averaged 0.20ml/s over 53 seconds
@20:02:54.71 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi
@20:02:55.29 Relieved excess vacuum in .6 seconds
@20:02:55.32 SamplePump.seek 1000ml,33:52
@20:03:29.15 <sampling> Cartridge 46 filtering 606/1000ml at 0.20ml/s with 26.1psi vacuum 3.4m deep (19.8psia)
@20:04:08.44 <FILTERING> Flow averaged 0.20ml/s over 1:14
@20:04:08.48 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi
@20:04:09.05 Relieved excess vacuum in .6 seconds
@20:04:09.07 SamplePump.seek 1000ml,32:39
@20:04:29.16 <sampling> Cartridge 46 filtering 618/1000ml at 0.20ml/s with 24.9psi vacuum 2.2m deep (18.0psia)
@20:04:56.36 <FILTERING> Flow averaged 0.20ml/s over 48 seconds
@20:04:56.40 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi
@20:04:57.09 Relieved excess vacuum in .7 seconds
@20:04:57.11 SamplePump.seek 1000ml,31:51
@20:05:29.16 <sampling> Cartridge 46 filtering 630/1000ml at 0.20ml/s with 25.9psi vacuum 3.8m deep (20.3psia)
@20:05:41.01 <FILTERING> Flow averaged 0.20ml/s over 44 seconds
@20:05:41.05 Waiting up to 12 seconds for vacuum to reduce from 27.7 to 18.0psi
@20:05:41.62 Relieved excess vacuum in .6 seconds
@20:05:41.66 SamplePump.seek 1000ml,31:07
@20:06:17.60 Flow averaged 0.20ml/s over 36 seconds
@20:06:17.64 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi
@20:06:18.22 Relieved excess vacuum in .6 seconds
@20:06:18.24 SamplePump.seek 1000ml,30:31
@20:06:29.16 <sampling> Cartridge 46 filtering 642/1000ml at 0.20ml/s with 27.0psi vacuum 2.5m deep (18.3psia)
@20:07:07.24 <FILTERING> Flow averaged 0.20ml/s over 50 seconds
@20:07:07.27 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi
@20:07:07.85 Relieved excess vacuum in .6 seconds
@20:07:07.87 SamplePump.seek 1000ml,29:41
@20:07:29.15 <sampling> Cartridge 46 filtering 654/1000ml at 0.20ml/s with 28.6psi vacuum 2.3m deep (18.1psia)
@20:07:35.61 <FILTERING> Flow averaged 0.20ml/s over 28 seconds
@20:07:35.65 Waiting up to 13 seconds for vacuum to reduce from 27.8 to 18.0psi
@20:07:36.22 Relieved excess vacuum in .6 seconds
@20:07:36.24 SamplePump.seek 1000ml,29:14
@20:08:04.21 Flow averaged 0.20ml/s over 28 seconds
@20:08:04.25 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi
@20:08:04.82 Relieved excess vacuum in .6 seconds
@20:08:04.84 SamplePump.seek 1000ml,28:46
@20:08:27.83 Flow averaged 0.20ml/s over 23 seconds
@20:08:27.87 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi
@20:08:28.44 Relieved excess vacuum in .6 seconds
@20:08:28.47 SamplePump.seek 1000ml,28:23
@20:08:29.15 <sampling> Cartridge 46 filtering 666/1000ml at 0.22ml/s with 22.3psi vacuum 3.9m deep (20.4psia)
@20:08:57.80 <FILTERING> Flow averaged 0.20ml/s over 30 seconds
@20:08:57.84 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi
@20:08:58.42 Relieved excess vacuum in .6 seconds
@20:08:58.44 SamplePump.seek 1000ml,27:53
@20:09:05.53 Flow estimate reduced to 0.16ml/s
@20:09:05.55 <closeIntake> Intake.close
@20:09:05.60 <closeExhaust> Exhaust.close
@20:09:05.79 <FILTERING> Toroid.in :hold
@20:09:05.85 Toroid.seek :bypass
@20:09:07.48 Toroid.in :default
@20:09:07.54 Toroid.seek :clear
@20:09:09.15 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@20:09:09.16 Pressure.stop
@20:09:09.36 Sampled  672.7ml
@20:09:11.43 <LRAUV> -> Cmd.startProcessing
@20:09:12.85 <PROCESSING> SPR.command \"read_sequences\"
@20:09:13.15 SPR.command \"stats_on\"
@20:09:13.26 SPR.command \"start 0 1\"
@20:09:14.00 <SPRlogger> SPR.start /var/log/esp/spr22may2120h.spr.bz2
@20:09:16.00 <PROCESSING> Handoff.select :waste
@20:09:22.68 Syringe tare is 18% of full scale
@20:09:22.70 PinPort.in :locking
@20:09:22.77 PinPort.seek :locked
@20:09:23.26 <posArchive> Archive.in :fast
@20:09:23.30 <posLyS2> Lysis2.in :fast
@20:09:23.42 <PROCESSING> Lysis1.seek 1350uL
@20:09:23.46 <posArchive> Archive.seek :full
@20:09:23.48 <posLyS2> Lysis2.seek :full
@20:09:25.48 <PROCESSING> Plunge.seek :reagent
@20:09:26.35 Evacuating puck with filter bubble point of 50psi
@20:09:26.37 PinPort.seek :locked
@20:09:26.46 Handoff.select :waste
@20:09:33.04 Air syringe pumping up to 1 stroke to reach 8psi
@20:09:33.05 Air stroke #1 of 1
@20:09:33.14 Air.in :pressurize
@20:09:33.20 Air.seek :pressurized
@20:09:44.77 1.3psi after pressurize
@20:09:44.79 Air.in :compress
@20:09:44.89 Air.seek :evac
@20:09:48.97 1.0psi after compress
@20:10:01.54 0.0psi after compress (stable)
@20:10:01.55 Air.in :default
@20:10:01.62 Air.hold
@20:10:01.79 at -0.0psi Opened to waste
@20:10:01.80 Handoff.select :blocked
@20:10:01.97 Archive syringe pumping up to 8 strokes to reach 50psi
@20:10:01.98 Archive stroke #1 of 8
@20:10:02.07 Archive.in :pressurize
@20:10:02.13 Archive.seek :pressurized
@20:10:14.99 6.8psi after pressurize
@20:10:15.01 Archive.in :compress
@20:10:15.10 Archive.seek :empty
@20:10:29.80 26.2psi after compress
@20:10:44.37 30.2psi after compress (stable)
@20:10:44.39 Archive.in :gulp
@20:10:44.49 Archive.seek :topVent
@20:10:51.64 30.5psi after gulp
@20:11:04.21 30.4psi after gulp (stable)
@20:11:04.22 Archive stroke #2 of 8
@20:11:04.34 Archive.in :pressurize
@20:11:04.41 Archive.seek :pressurized
@20:11:17.25 30.3psi after pressurize
@20:11:17.27 Archive.in :compress
@20:11:17.35 Archive.seek :empty
@20:11:32.03 47.5psi after compress
@20:11:34.67 Reached 50.1 of 50.0psi after pumping 3460uL (2.00 strokes)
@20:11:34.68 Archive.in :default
@20:11:34.76 Archive.hold
@20:11:34.83 Handoff.select :waste
@20:11:42.01 delay 1:00
@20:12:42.02 Handoff.select :blocked
@20:13:19.19 Handoff.select :waste
@20:13:19.31 6.6psi at evacEnd
@20:13:19.32 Handoff.select :blocked
@20:13:19.46 Archive.in :fast
@20:13:19.58 Archive.seek :topVent
@20:13:45.25 Stable @5.4psi after Handoff blocked
@20:13:45.27 Archive syringe pumping up to 8 strokes to reach 35psi
@20:13:45.29 Archive stroke #1 of 8
@20:13:45.38 Archive.in :pressurize
@20:13:45.44 Archive.seek :pressurized
@20:13:58.28 7.0psi after pressurize
@20:13:58.32 Archive.in :compress
@20:13:58.42 Archive.seek :empty
@20:14:13.09 11.2psi after compress
@20:14:50.16 23.7psi after compress (stable)
@20:14:50.18 Archive.in :gulp
@20:14:50.26 Archive.seek :topVent
@20:14:57.41 24.7psi after gulp
@20:15:09.98 25.4psi after gulp (stable)
@20:15:09.99 Archive stroke #2 of 8
@20:15:10.09 Archive.in :pressurize
@20:15:10.15 Archive.seek :pressurized
@20:15:23.02 26.3psi after pressurize
@20:15:23.04 Archive.in :compress
@20:15:23.12 Archive.seek :empty
@20:15:37.80 29.4psi after compress
@20:15:47.68 Reached 35.5 of 35.4psi after pumping 3460uL (2.00 strokes)
@20:15:47.69 Archive.in :default
@20:15:47.76 Archive.hold
@20:15:47.83 Handoff.select :waste
@20:16:02.02 delay 1:00
@20:17:02.03 Handoff.select :blocked
@20:17:24.71 Handoff.select :waste
@20:17:24.82 1.6psi at evacEnd
@20:17:24.84 Air.seek :fullVent
@20:17:26.26 Archive.in :fast
@20:17:26.33 Archive.seek :topVent
@20:17:33.42 Seawater evacuated, Presure above/below filter released. Lysis begins
@20:17:33.51 Lysis1.in :slow
@20:17:33.58 Lysis1.seek 1050uL
@20:18:03.58 delay 5 seconds
@20:18:08.60 Handoff.select :blocked
@20:18:08.78 Lysis1.seek 450uL
@20:19:08.72 delay 3 seconds
@20:19:11.79 Air.in mix with 1.40048 parts Lysis1
@20:19:12.29 <Airmixer> Air.seek :evac
@20:19:12.34 <PROCESSING> Lysis1.seek 300uL
@20:19:27.48 Lysis1.seek :empty
@20:19:57.58 Lysis buffer delivered @18.5psi
@20:19:57.60 Polling Can every 2:00
@20:19:58.14 PuckHeater.configure :default #ambient 14.0C
@20:19:58.20 PuckHeater.seek 80.1C
@20:21:09.52 PuckHeater.hold 2:00,80.1C
@20:23:09.27 Polling Can every 2:00
@20:23:09.81 PuckHeater.configure :default #ambient 14.1C
@20:23:09.86 PuckHeater.hold 30 seconds,79.1C
@20:23:39.95 Polling Can every 2:00
@20:23:40.49 PuckHeater.configure :default #ambient 14.2C
@20:23:40.54 PuckHeater.hold 30 seconds,78.6C
@20:24:10.63 Polling Can every 2:00
@20:24:11.17 PuckHeater.configure :default #ambient 14.1C
@20:24:11.25 PuckHeater.hold 10:00,78C
@20:34:09.26 Lysis complete. Syringe pressure @21.0psi
@20:34:09.27 Handoff.select :blocked
@20:34:09.33 Archive syringe pumping up to 5 strokes to reach 40psi
Archive stroke #1 of 5
@20:34:09.42 Archive.in :pressurize
@20:34:09.48 Archive.seek :pressurized
@20:34:22.66 20.5psi after pressurize
@20:34:22.68 Archive.in :compress
@20:34:22.76 Archive.seek :empty
@20:34:31.31 Reached 40.8 of 40.0psi after pumping 1598uL (0.92 strokes)
@20:34:31.32 Archive.in :default
@20:34:31.39 Archive.hold
@20:34:31.46 delay 2 seconds
@20:34:33.49 Archive.in :fast
@20:34:33.55 Archive.seek :full
@20:34:40.30 Lysis1.seek 138uL
@20:34:54.16 delay 1 second
@20:34:55.19 <fillArchive> Archive.seek :full
@20:34:55.22 <PROCESSING> Lysis1.seek :empty
@20:35:09.21 Initial air pressure @48.7psi
@20:35:09.27 Lysis1.in :extract
@20:35:09.36 Lysis1.seek 600uL,nil
@20:35:12.85 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@20:35:12.86 Archive stroke #1 of 6
@20:35:12.94 Archive.in :pressurize
@20:35:13.00 Archive.seek :pressurized
@20:35:25.84 15.6psi after pressurize
@20:35:25.86 Archive.in :compress
@20:35:25.93 Archive.seek :empty
@20:35:38.26 Archive.in :default
@20:35:38.31 Archive.hold
@20:35:38.45 <PROCESSING> Initial 600uL lysate @18.0psi
@20:35:38.46 Handoff.select :blocked
@20:35:38.56 Initial air pressure @19.1psi
@20:35:38.64 Lysis1.seek 825uL,nil
@20:35:40.40 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@20:35:40.41 Archive stroke #1 of 6
@20:35:40.51 Archive.in :compress
@20:35:40.60 Archive.seek :empty
@20:35:42.94 16.1psi after compress
@20:35:45.06 Archive.in :default
@20:35:45.11 Archive.hold
@20:35:45.30 <PROCESSING> Recovered 825uL lysate @16.6psi
@20:35:45.32 Air.in :halfSpeed
@20:35:45.39 Air.seek :fullVent
@20:35:47.59 Puck Top Vented, bottom @21.8psi
@20:35:47.60 Handoff.select :waste
@20:35:47.73 delay 5 seconds
@20:35:52.79 Handoff open to waste, bottom @-0.2psi
@20:35:52.81 Air.in :default
@20:35:52.87 Air.seek :evac
@20:35:54.28 Lysis2.in :DAmix
@20:35:54.39 Lysis1.in :DAmix
@20:35:54.82 <Lysis2mixer> Lysis2.seek 1480uL
@20:35:54.87 <PROCESSING> Lysis1.seek 575uL
@20:36:29.94 delay 5 seconds
@20:36:34.97 Handoff.select SPR
@20:36:35.09 SPR.command \"start 2 14\"
@20:36:36.08 SPR stabilized
@20:36:36.21 delay 10 seconds
@20:36:46.22 SPR.command \"start 1 0\"
@20:36:49.44 Waiting up to 4:00 for SPR to enter state 1
@20:38:51.81 SPR.command \"hold_on\"
@20:38:53.23 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@20:38:53.68 <Lysis2mixer> Lysis2.seek 1180uL
@20:38:53.73 <PROCESSING> Lysis1.seek 275uL
@20:39:35.73 delay 15 seconds
@20:39:50.75 SPR.command \"hold_off\"
@20:39:53.88 Waiting up to 38:00 for SPR to enter state 7
@21:00:43.71 <SPRlogger> SPRsummary:0.261333,110.372,111.507,107.509 after 3239s
@21:07:20.93 <PROCESSING> SPR analysis completed
@21:07:26.59 <SPRlogger> Ended log spr22may2120h.spr.bz2
@21:07:26.60 <PROCESSING> SPR.command \"stats_off\"
@21:07:28.14 <LRAUV> -> Cmd.stop
@21:07:28.24 <closeHandoff> Handoff.select :close
@21:07:28.37 <LRAUV> Gate.power :heat,:OFF
@21:07:28.39 Gate.power :cartridge,:OFF
@21:07:28.47 Carousel.seek 46
@21:07:28.65 Plunge.seek :locked
@21:07:29.51 PinPort.in :unlocking
@21:07:29.57 PinPort.seek :clear
@21:07:30.12 <Archive> Archive.in :unlocking
@21:07:30.24 Archive.seek :clear
@21:07:30.33 <Air> Air.in :unlocking
@21:07:30.44 Air.seek :clear
@21:07:30.53 <Lysis1> Lysis1.in :unlocking
@21:07:30.63 Lysis1.seek :clear
@21:07:30.72 <Lysis2> Lysis2.in :unlocking
@21:07:30.78 Lysis2.seek :clear
@21:07:34.36 <LRAUV> Gate.power :motor,:OFF
@21:07:34.38 Gate.power :core,:OFF
@21:07:34.40 Gate.power :analytic1,:OFF
@21:07:34.43 Gate.power :analytic2,:OFF
@21:07:34.45 Gate.power :analytic3,:OFF
@21:07:34.47 Gate.power :main,:OFF
@21:07:34.50 Safely stopped and ready to power off
##-- file closed 2022-05-22T07:04:25.173Z

##-- file opened 2022-05-22T07:04:25.175Z
@00:04:25.21 -> showStatus
@00:04:27.23 -> Cmd.loadCartridge -10
@00:04:27.30 Gate.power :main,:ON,limit to 5000mA
@00:04:27.32 Gate.power :analytic1,:ON,limit to 5000mA
@00:04:27.34 Gate.power :analytic2,:ON,limit to 5000mA
@00:04:27.37 Gate.power :analytic3,:ON,limit to 5000mA
@00:04:27.51 Powering core bus
@00:04:29.77 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@00:04:30.24 <LRAUV> Archive.configure :default
@00:04:30.37 Air.configure :default
@00:04:30.47 Lysis1.configure :default
@00:04:30.58 Lysis2.configure :default
@00:04:30.71 Plunge.configure :default
@00:04:30.90 SamplePump.configure :default
@00:04:31.15 Toroid.configure :default
@00:04:31.41 Carousel.configure :default
@00:04:31.74 Pressure.configure :default
@00:04:32.04 ExtraPump.configure :default
@00:04:32.29 PinPort.configure :default
@00:04:32.38 All elf controllers running firmware version 4.79
@00:04:32.40 Gate.power :motor,:ON,limit to 5000mA
@00:04:32.59 <Archive_home> Archive.in :default
@00:04:32.89 Archive.home.jog 0
@00:04:33.05 <PinPort_home> PinPort.in :default
@00:04:33.09 <Toroid_home> Toroid.in :default
@00:04:33.16 <Lysis2_home> Lysis2.in :default
@00:04:33.19 <Lysis1_home> Lysis1.in :default
@00:04:33.26 <Plunge_home> Plunge.in :default
@00:04:33.31 <Air_home> Air.in :default
@00:04:33.44 <PinPort_home> PinPort.home.jog 0
@00:04:33.53 <Lysis2_home> Lysis2.home.jog 0
@00:04:33.55 <Lysis1_home> Lysis1.home.jog 0
@00:04:33.59 <Toroid_home> Toroid.home.jog 0
@00:04:33.63 <Plunge_home> Plunge.home.jog 0
@00:04:33.68 <Air_home> Air.home.jog 0
@00:04:34.05 <Archive_home> Archive.in :homing
@00:04:34.21 Archive.jog 1399
@00:04:34.43 <Toroid_home> Toroid.in :homing
@00:04:34.46 <PinPort_home> PinPort.in :homing
@00:04:34.50 <Plunge_home> Plunge.in :homing
@00:04:34.53 <Lysis2_home> Lysis2.in :homing
@00:04:34.58 <Air_home> Air.in :homing
@00:04:34.62 <Lysis1_home> Lysis1.in :homing
@00:04:34.81 <Toroid_home> Toroid.jog 649
@00:04:34.85 <PinPort_home> PinPort.jog -105
@00:04:34.89 <Plunge_home> Plunge.jog 1564
@00:04:34.91 <Lysis2_home> Lysis2.jog 1404
@00:04:34.97 <Air_home> Air.jog 1391
@00:04:35.00 <Lysis1_home> Lysis1.jog 1382
@00:04:35.25 <PinPort_home> PinPort.setRawPosition! 0
@00:04:35.30 PinPort.seek :home
@00:04:35.40 PinPort.in :default
@00:04:35.42 <Archive_home> Archive.setRawPosition! 50
@00:04:35.50 Archive.seek :home
@00:04:35.79 Archive.in :default
@00:04:36.20 <Lysis2_home> Lysis2.setRawPosition! 50
@00:04:36.27 Lysis2.seek :home
@00:04:36.37 <Lysis1_home> Lysis1.setRawPosition! 50
@00:04:36.40 <Plunge_home> Plunge.setRawPosition! 50
@00:04:36.43 <Air_home> Air.setRawPosition! 50
@00:04:36.51 <Lysis1_home> Lysis1.seek :home
@00:04:36.56 <Plunge_home> Plunge.seek :home
@00:04:36.62 <Air_home> Air.seek :home
@00:04:36.79 <Lysis2_home> Lysis2.in :default
@00:04:36.89 <Lysis1_home> Lysis1.in :default
@00:04:36.99 <Plunge_home> Plunge.in :default
@00:04:37.07 <Air_home> Air.in :default
@00:04:37.16 <Toroid_home> Toroid.setRawPosition! 0
@00:04:37.20 Toroid.seek :home
@00:04:37.30 Toroid.in :default
@00:04:37.48 <LRAUV> Toroid.seek :clear
@00:04:39.09 Carousel.in :default
@00:04:39.15 Carousel.home.jog 0
@00:04:39.64 Carousel.in :default
@00:04:39.74 Carousel.seek 46
@00:04:39.90 Selecting Cartridge 45
@00:04:40.16 Carousel.seek 45
@00:04:40.65 Lysis1.in :fast
@00:04:40.72 Lysis1.seek :locked
@00:04:43.32 <Plunge> Plunge.seek :locked
@00:04:43.45 <Air> Air.in :fast
@00:04:43.51 Air.seek :locked
@00:04:45.97 <LRAUV> Powering cartridge bus
@00:04:48.57 Gate.setIR 1750,5000,0,6
@00:04:48.69 Gate.setIR 0,5000,0,6
@00:04:48.76 PuckHeater.configure :default
@00:04:48.81 Gate.power :heat,:ON,limit to 5000mA
@00:04:49.49 Mounted lysis Cartridge board#Q05086
@00:04:49.52 Using default puck heat loss curve for HeatingAccelInCarousel
@00:05:07.63 -> Cmd.startFiltering
@00:05:07.87 <FILTERING> Preparing to filter cartridge 45
@00:05:08.15 Priming sample loop w/100ml, bypass w/10ml
@00:05:08.16 SamplePump.setPosition! 0ml
@00:05:08.21 Bypass.open
@00:05:09.67 Intake.open
@00:05:10.37 Sea 7.5m deep (25.8psia)
@00:05:10.39 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@00:05:12.96 Vacuum tare is 54% of full scale
@00:05:13.09 Pressure.in :default
@00:05:13.15 Pressure.hold
@00:05:13.33 SamplePump.in :prime
@00:05:13.40 SamplePump.seek 10ml,59 seconds
@00:05:22.02 Pressure.stop
@00:05:22.07 SamplePump.setPosition! 0ml
@00:05:22.12 Exhaust.open
@00:05:23.56 <closeBypass> Bypass.close
@00:05:25.06 <FILTERING> Pressure.hold
@00:05:25.18 Sea 7.6m deep (25.9psia)
@00:05:25.32 SamplePump.in :prime
@00:05:25.39 SamplePump.seek 100ml,5:16
@00:06:59.81 Pressure.stop
@00:06:59.87 Bypass.open
@00:07:00.52 Intake.close
@00:07:01.24 Toroid.in :hold
@00:07:01.30 Toroid.seek :crack
@00:07:02.81 Toroid.seek :cartridge
@00:07:03.90 Toroid.in :default
@00:07:03.96 Toroid.seek :clear
@00:07:05.92 Pressurizing cartridge to 26psi above 25.9psia
@00:07:05.93 SamplePump.setPosition! 0ml
@00:07:05.98 Intake.open
@00:07:06.65 Bypass.close
@00:07:07.32 Exhaust.close
@00:07:08.15 Pressure.in :default
@00:07:08.21 Pressure.hold
@00:07:08.39 SamplePump.in :charge
@00:07:08.46 SamplePump.seek 2.3ml,42 seconds
@00:07:17.94 Pressure.stop
@00:07:18.00 Intake.close
@00:07:18.75 Pressurized cartridge with 0.432ml to 27.7psi
@00:07:18.76 Isolated @49.4psia (23.5psi)
@00:07:18.77 Verifying cartridge holds at least 0.65..0.85 of 49.4psia
@00:07:18.81 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@00:07:23.44 Sampling 1000.0ml
@00:07:23.45 SamplePump.setPosition! 0ml
@00:07:23.51 Exhaust.open
@00:07:24.25 Intake.open
@00:07:25.08 Pressure.in :default
@00:07:25.14 Pressure.hold
@00:07:25.26 Sea 7.8m deep (26.2psia)
@00:07:25.40 SamplePump.in :sample
@00:07:25.47 SamplePump.seek 1000ml,1:23:48
@00:07:28.12 <sampling> Cartridge 45 filtering 1/1000ml at 0.41ml/s with 21.6psi vacuum 7.2m deep (25.4psia)
@00:08:28.13 Cartridge 45 filtering 25/1000ml at 0.37ml/s with 25.8psi vacuum 6.3m deep (24.0psia)
@00:09:28.12 Cartridge 45 filtering 48/1000ml at 0.38ml/s with 27.1psi vacuum 6.6m deep (24.4psia)
@00:10:28.12 Cartridge 45 filtering 69/1000ml at 0.34ml/s with 27.0psi vacuum 5.7m deep (23.2psia)
@00:11:28.12 Cartridge 45 filtering 89/1000ml at 0.31ml/s with 23.4psi vacuum 5.7m deep (23.1psia)
@00:12:28.13 Cartridge 45 filtering 109/1000ml at 0.30ml/s with 24.1psi vacuum 6.9m deep (24.9psia)
@00:13:28.18 Cartridge 45 filtering 128/1000ml at 0.34ml/s with 25.1psi vacuum 5.7m deep (23.2psia)
@00:14:28.12 Cartridge 45 filtering 146/1000ml at 0.27ml/s with 24.1psi vacuum 5.5m deep (22.8psia)
@00:15:28.13 Cartridge 45 filtering 164/1000ml at 0.30ml/s with 25.6psi vacuum 7.2m deep (25.4psia)
@00:16:28.12 Cartridge 45 filtering 181/1000ml at 0.29ml/s with 25.3psi vacuum 5.4m deep (22.7psia)
@00:17:28.13 Cartridge 45 filtering 197/1000ml at 0.25ml/s with 26.3psi vacuum 5.1m deep (22.3psia)
@00:18:28.12 Cartridge 45 filtering 213/1000ml at 0.29ml/s with 23.7psi vacuum 8.4m deep (27.1psia)
@00:19:28.12 Cartridge 45 filtering 229/1000ml at 0.24ml/s with 25.4psi vacuum 5.2m deep (22.4psia)
@00:20:28.12 Cartridge 45 filtering 244/1000ml at 0.24ml/s with 24.8psi vacuum 6.3m deep (24.1psia)
@00:21:28.12 Cartridge 45 filtering 259/1000ml at 0.25ml/s with 26.4psi vacuum 4.8m deep (21.8psia)
@00:22:28.12 Cartridge 45 filtering 273/1000ml at 0.26ml/s with 21.8psi vacuum 6.0m deep (23.5psia)
@00:23:28.12 Cartridge 45 filtering 287/1000ml at 0.23ml/s with 22.2psi vacuum 4.8m deep (21.8psia)
@00:24:28.12 Cartridge 45 filtering 301/1000ml at 0.22ml/s with 23.0psi vacuum 5.1m deep (22.2psia)
@00:25:28.12 Cartridge 45 filtering 314/1000ml at 0.21ml/s with 22.9psi vacuum 5.7m deep (23.1psia)
@00:26:28.12 Cartridge 45 filtering 328/1000ml at 0.24ml/s with 24.0psi vacuum 6.0m deep (23.6psia)
@00:27:28.12 Cartridge 45 filtering 341/1000ml at 0.21ml/s with 24.3psi vacuum 4.9m deep (21.9psia)
@00:28:28.12 Cartridge 45 filtering 353/1000ml at 0.23ml/s with 23.6psi vacuum 5.6m deep (22.9psia)
@00:29:28.12 Cartridge 45 filtering 366/1000ml at 0.20ml/s with 24.8psi vacuum 4.5m deep (21.4psia)
@00:30:28.12 Cartridge 45 filtering 379/1000ml at 0.20ml/s with 24.9psi vacuum 4.3m deep (21.1psia)
@00:31:28.12 Cartridge 45 filtering 391/1000ml at 0.20ml/s with 25.8psi vacuum 4.5m deep (21.3psia)
@00:32:28.12 Cartridge 45 filtering 403/1000ml at 0.22ml/s with 25.6psi vacuum 4.5m deep (21.4psia)
@00:33:28.13 Cartridge 45 filtering 416/1000ml at 0.20ml/s with 24.4psi vacuum 5.5m deep (22.8psia)
@00:34:28.12 Cartridge 45 filtering 428/1000ml at 0.20ml/s with 28.7psi vacuum 4.3m deep (21.1psia)
@00:35:28.12 Cartridge 45 filtering 440/1000ml at 0.20ml/s with 27.5psi vacuum 3.9m deep (20.4psia)
@00:36:28.12 Cartridge 45 filtering 452/1000ml at 0.20ml/s with 26.5psi vacuum 4.8m deep (21.8psia)
@00:37:08.06 <FILTERING> Flow averaged 0.26ml/s over 29:43
@00:37:08.09 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi
@00:37:08.93 Relieved excess vacuum in .9 seconds
@00:37:08.95 SamplePump.seek 1000ml,45:29
@00:37:28.11 <sampling> Cartridge 45 filtering 464/1000ml at 0.20ml/s with 27.3psi vacuum 4.7m deep (21.6psia)
@00:38:19.32 <FILTERING> Flow averaged 0.20ml/s over 1:11
@00:38:19.36 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@00:38:20.19 Relieved excess vacuum in .9 seconds
@00:38:20.21 SamplePump.seek 1000ml,44:19
@00:38:28.12 <sampling> Cartridge 45 filtering 476/1000ml at 0.20ml/s with 26.4psi vacuum 4.0m deep (20.7psia)
@00:39:07.71 <FILTERING> Flow averaged 0.20ml/s over 48 seconds
@00:39:07.74 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi
@00:39:08.57 Relieved excess vacuum in .9 seconds
@00:39:08.60 SamplePump.seek 1000ml,43:31
@00:39:12.03 Flow estimate reduced to 0.16ml/s
@00:39:12.05 <closeIntake> Intake.close
@00:39:12.11 <closeExhaust> Exhaust.close
@00:39:12.30 <FILTERING> Toroid.in :hold
@00:39:12.36 Toroid.seek :bypass
@00:39:13.98 Toroid.in :default
@00:39:14.04 Toroid.seek :clear
@00:39:15.65 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@00:39:15.66 Pressure.stop
@00:39:15.85 Sampled  484.3ml
@00:39:18.02 <LRAUV> -> Cmd.startProcessing
@00:39:19.45 <PROCESSING> SPR.command \"read_sequences\"
@00:39:19.92 SPR.command \"stats_on\"
@00:39:20.96 SPR.command \"start 0 1\"
@00:39:21.70 <SPRlogger> SPR.start /var/log/esp/spr22may2200h.spr.bz2
@00:39:24.21 <PROCESSING> Handoff.select :waste
@00:39:30.88 Syringe tare is 18% of full scale
@00:39:30.90 PinPort.in :locking
@00:39:30.98 PinPort.seek :locked
@00:39:31.46 <posArchive> Archive.in :fast
@00:39:31.52 <posLyS2> Lysis2.in :fast
@00:39:31.58 <posArchive> Archive.seek :full
@00:39:31.64 <PROCESSING> Lysis1.seek 1350uL
@00:39:31.74 <posLyS2> Lysis2.seek :full
@00:39:33.74 <PROCESSING> Plunge.seek :reagent
@00:39:34.65 Evacuating puck with filter bubble point of 50psi
@00:39:34.67 PinPort.seek :locked
@00:39:34.77 Handoff.select :waste
@00:39:41.85 Air syringe pumping up to 1 stroke to reach 8psi
@00:39:41.86 Air stroke #1 of 1
@00:39:41.95 Air.in :pressurize
@00:39:42.02 Air.seek :pressurized
@00:39:53.59 1.1psi after pressurize
@00:39:53.61 Air.in :compress
@00:39:53.68 Air.seek :evac
@00:39:57.77 0.8psi after compress
@00:40:10.34 -0.1psi after compress (stable)
@00:40:10.35 Air.in :default
@00:40:10.42 Air.hold
@00:40:10.63 at -0.2psi Opened to waste
@00:40:10.64 Handoff.select :blocked
@00:40:10.77 Archive syringe pumping up to 8 strokes to reach 50psi
@00:40:10.78 Archive stroke #1 of 8
@00:40:10.87 Archive.in :pressurize
@00:40:10.94 Archive.seek :pressurized
@00:40:23.83 -0.1psi after pressurize
@00:40:23.85 Archive.in :compress
@00:40:23.93 Archive.seek :empty
@00:40:38.61 28.7psi after compress
@00:40:51.68 31.3psi after compress (stable)
@00:40:51.70 Archive.in :gulp
@00:40:51.78 Archive.seek :topVent
@00:40:59.38 31.4psi after gulp
@00:41:11.95 30.9psi after gulp (stable)
@00:41:11.96 Archive stroke #2 of 8
@00:41:12.08 Archive.in :pressurize
@00:41:12.15 Archive.seek :pressurized
@00:41:25.01 30.2psi after pressurize
@00:41:25.02 Archive.in :compress
@00:41:25.10 Archive.seek :empty
@00:41:39.78 32.1psi after compress
@00:41:52.35 32.0psi after compress (stable)
@00:41:52.37 Archive.in :gulp
@00:41:52.44 Archive.seek :topVent
@00:41:59.59 32.9psi after gulp
@00:42:12.16 32.8psi after gulp (stable)
@00:42:12.17 Archive stroke #3 of 8
@00:42:12.26 Archive.in :pressurize
@00:42:12.32 Archive.seek :pressurized
@00:42:25.19 32.2psi after pressurize
@00:42:25.21 Archive.in :compress
@00:42:25.28 Archive.seek :empty
@00:42:39.99 33.0psi after compress
@00:42:52.57 33.7psi after compress (stable)
@00:42:52.59 Archive.in :gulp
@00:42:52.66 Archive.seek :topVent
@00:42:59.84 34.9psi after gulp
@00:43:12.42 34.9psi after gulp (stable)
@00:43:12.43 Archive stroke #4 of 8
@00:43:12.52 Archive.in :pressurize
@00:43:12.59 Archive.seek :pressurized
@00:43:25.46 34.5psi after pressurize
@00:43:25.48 Archive.in :compress
@00:43:25.56 Archive.seek :empty
@00:43:40.24 36.3psi after compress
@00:44:14.66 Reached 50.1 of 50.0psi after pumping 6920uL (4.00 strokes)
@00:44:14.67 Archive.in :default
@00:44:14.75 Archive.hold
@00:44:14.83 Handoff.select :waste
@00:44:29.00 delay 1:00
@00:45:29.02 Handoff.select :blocked
@00:45:51.69 Handoff.select :waste
@00:45:51.80 2.3psi at evacEnd
@00:45:51.81 Handoff.select :blocked
@00:45:51.97 Archive.in :fast
@00:45:52.05 Archive.seek :topVent
@00:46:05.70 Stable @1.5psi after Handoff blocked
@00:46:05.73 Archive syringe pumping up to 8 strokes to reach 31psi
@00:46:05.74 Archive stroke #1 of 8
@00:46:05.84 Archive.in :pressurize
@00:46:05.90 Archive.seek :pressurized
@00:46:19.13 3.1psi after pressurize
@00:46:19.15 Archive.in :compress
@00:46:19.23 Archive.seek :empty
@00:46:33.92 6.3psi after compress
@00:47:04.49 13.9psi after compress (stable)
@00:47:04.51 Archive.in :gulp
@00:47:04.59 Archive.seek :topVent
@00:47:11.75 15.2psi after gulp
@00:47:24.32 16.5psi after gulp (stable)
@00:47:24.33 Archive stroke #2 of 8
@00:47:24.42 Archive.in :pressurize
@00:47:24.49 Archive.seek :pressurized
@00:47:37.34 18.6psi after pressurize
@00:47:37.36 Archive.in :compress
@00:47:37.44 Archive.seek :empty
@00:47:52.16 21.4psi after compress
@00:48:23.23 27.2psi after compress (stable)
@00:48:23.25 Archive.in :gulp
@00:48:23.36 Archive.seek :topVent
@00:48:30.51 28.6psi after gulp
@00:48:49.11 Reached 31.5 of 31.5psi after pumping 3460uL (2.00 strokes)
@00:48:49.12 Archive.in :default
@00:48:49.20 Archive.hold
@00:48:49.27 Handoff.select :waste
@00:48:56.48 delay 1:00
@00:49:56.49 Handoff.select :blocked
@00:50:20.67 Handoff.select :waste
@00:50:20.78 3.1psi at evacEnd
@00:50:20.80 Air.seek :fullVent
@00:50:22.23 Archive.in :fast
@00:50:22.30 Archive.seek :topVent
@00:50:22.38 Seawater evacuated, Presure above/below filter released. Lysis begins
@00:50:22.47 Lysis1.in :slow
@00:50:22.56 Lysis1.seek 1050uL
@00:50:52.59 delay 5 seconds
@00:50:57.60 Handoff.select :blocked
@00:50:57.78 Lysis1.seek 450uL
@00:51:57.77 delay 3 seconds
@00:52:00.86 Air.in mix with 1.40048 parts Lysis1
@00:52:01.29 <Airmixer> Air.seek :evac
@00:52:01.32 <PROCESSING> Lysis1.seek 300uL
@00:52:16.44 Lysis1.seek :empty
@00:52:46.53 Lysis buffer delivered @18.3psi
@00:52:46.55 Polling Can every 2:00
@00:52:47.09 PuckHeater.configure :default #ambient 13.9C
@00:52:47.14 PuckHeater.seek 80.1C
@00:53:52.24 PuckHeater.hold 2:00,80.1C
@00:55:52.14 Polling Can every 2:00
@00:55:52.67 PuckHeater.configure :default #ambient 14.0C
@00:55:52.72 PuckHeater.hold 30 seconds,79.1C
@00:56:22.87 Polling Can every 2:00
@00:56:23.42 PuckHeater.configure :default #ambient 14.0C
@00:56:23.47 PuckHeater.hold 30 seconds,78.6C
@00:56:53.60 Polling Can every 2:00
@00:56:54.14 PuckHeater.configure :default #ambient 14.0C
@00:56:54.19 PuckHeater.hold 10:00,78C
@01:06:53.05 Lysis complete. Syringe pressure @21.2psi
@01:06:53.06 Handoff.select :blocked
@01:06:53.11 Archive syringe pumping up to 5 strokes to reach 40psi
@01:06:53.12 Archive stroke #1 of 5
@01:06:53.22 Archive.in :pressurize
@01:06:53.29 Archive.seek :pressurized
@01:07:06.15 20.7psi after pressurize
@01:07:06.17 Archive.in :compress
@01:07:06.25 Archive.seek :empty
@01:07:13.98 Reached 40.6 of 40.0psi after pumping 1580uL (0.91 strokes)
@01:07:13.99 Archive.in :default
@01:07:14.06 Archive.hold
@01:07:14.43 delay 2 seconds
@01:07:16.45 Archive.in :fast
@01:07:16.51 Archive.seek :full
@01:07:23.17 Lysis1.seek 138uL
@01:07:37.10 delay 1 second
@01:07:38.12 <fillArchive> Archive.seek :full
@01:07:38.23 <PROCESSING> Lysis1.seek :empty
@01:07:52.17 Initial air pressure @44.7psi
@01:07:52.23 Lysis1.in :extract
@01:07:52.35 Lysis1.seek 600uL,nil
@01:07:56.24 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@01:07:56.25 Archive stroke #1 of 6
@01:07:56.39 Archive.in :pressurize
@01:07:56.46 Archive.seek :pressurized
@01:08:09.35 14.2psi after pressurize
@01:08:09.39 Archive.in :compress
@01:08:09.48 Archive.seek :empty
@01:08:17.31 Syringe::LysatePlungerStuck in PROCESSING -- at < 378uL during Lysis Syringe #1 (LyS1) move
@01:08:17.34 <PROCESSING> Lysis1.in :default
@01:08:17.40 Lysis1.seek 342uL
@01:08:18.14 <pump> Reached 26.2 of 25.0psi after pumping 1601uL (0.93 strokes)
@01:08:18.15 Archive.in :default
@01:08:18.22 Archive.hold
@01:08:19.21 <PROCESSING> Lysis1.in :extract
@01:08:19.27 Lysis1.seek 600uL,nil
@01:08:24.01 Initial 600uL lysate @19.5psi
@01:08:24.02 Handoff.select :blocked
@01:08:24.13 Initial air pressure @20.6psi
@01:08:24.20 Lysis1.seek 825uL,nil
@01:08:26.49 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@01:08:26.50 Archive stroke #1 of 6
@01:08:26.64 Archive.in :compress
@01:08:26.71 Archive.seek :empty
@01:08:30.77 Archive.in :default
@01:08:30.82 Archive.hold
@01:08:31.02 <PROCESSING> Recovered 825uL lysate @16.3psi
@01:08:31.04 Air.in :halfSpeed
@01:08:31.13 Air.seek :fullVent
@01:08:33.34 Puck Top Vented, bottom @4.9psi
@01:08:33.35 Handoff.select :waste
@01:08:33.47 delay 5 seconds
@01:08:38.54 Handoff open to waste, bottom @0.1psi
@01:08:38.55 Air.in :default
@01:08:38.62 Air.seek :evac
@01:08:40.04 Lysis2.in :DAmix
@01:08:40.14 Lysis1.in :DAmix
@01:08:40.58 <Lysis2mixer> Lysis2.seek 1480uL
@01:08:40.62 <PROCESSING> Lysis1.seek 575uL
@01:09:15.70 delay 5 seconds
@01:09:20.72 Handoff.select SPR
@01:09:20.83 SPR.command \"start 2 14\"
@01:09:21.74 SPR stabilized
@01:09:21.88 delay 10 seconds
@01:09:31.90 SPR.command \"start 1 0\"
@01:09:35.11 Waiting up to 4:00 for SPR to enter state 1
@01:11:37.48 SPR.command \"hold_on\"
@01:11:38.90 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@01:11:39.36 <Lysis2mixer> Lysis2.seek 1180uL
@01:11:39.44 <PROCESSING> Lysis1.seek 275uL
@01:12:21.45 delay 15 seconds
@01:12:36.46 SPR.command \"hold_off\"
@01:12:39.55 Waiting up to 38:00 for SPR to enter state 7
@01:33:29.38 <SPRlogger> SPRsummary:-10.316,95.3173,100.291,102.021 after 3406s
@01:40:06.60 <PROCESSING> SPR analysis completed
@01:40:13.04 <SPRlogger> Ended log spr22may2200h.spr.bz2
@01:40:13.05 <PROCESSING> SPR.command \"stats_off\"
@01:40:14.34 <LRAUV> -> Cmd.stop
@01:40:14.43 <closeHandoff> Handoff.select :close
@01:40:14.56 <LRAUV> Gate.power :heat,:OFF
@01:40:14.58 Gate.power :cartridge,:OFF
@01:40:14.66 Carousel.seek 45
@01:40:14.84 Plunge.seek :locked
@01:40:15.70 PinPort.in :unlocking
@01:40:15.76 PinPort.seek :clear
@01:40:16.30 <Archive> Archive.in :unlocking
@01:40:16.44 <Air> Air.in :unlocking
@01:40:16.47 <Archive> Archive.seek :clear
@01:40:16.59 <Air> Air.seek :clear
@01:40:16.70 <Lysis1> Lysis1.in :unlocking
@01:40:16.82 Lysis1.seek :clear
@01:40:16.91 <Lysis2> Lysis2.in :unlocking
@01:40:16.98 Lysis2.seek :clear
@01:40:20.59 <LRAUV> Gate.power :motor,:OFF
@01:40:20.61 Gate.power :core,:OFF
@01:40:20.63 Gate.power :analytic1,:OFF
@01:40:20.65 Gate.power :analytic2,:OFF
@01:40:20.67 Gate.power :analytic3,:OFF
@01:40:20.69 Gate.power :main,:OFF
@01:40:20.71 Safely stopped and ready to power off
##-- file closed 2022-05-23T02:32:23.814Z