##-- file opened 2022-05-20T21:33:47.391Z
@14:33:48.29 -> showStatus
@14:33:49.91 -> Cmd.loadCartridge -10
@14:33:49.98 Gate.power :main,:ON,limit to 5000mA
@14:33:49.99 Gate.power :analytic1,:ON,limit to 5000mA
@14:33:50.02 Gate.power :analytic2,:ON,limit to 5000mA
@14:33:50.04 Gate.power :analytic3,:ON,limit to 5000mA
@14:33:50.19 Powering core bus
@14:33:52.45 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@14:33:52.91 <LRAUV> Archive.configure :default
@14:33:53.04 Air.configure :default
@14:33:53.15 Lysis1.configure :default
@14:33:53.25 Lysis2.configure :default
@14:33:53.37 Plunge.configure :default
@14:33:53.50 SamplePump.configure :default
@14:33:53.63 Toroid.configure :default
@14:33:53.75 Carousel.configure :default
@14:33:53.88 Pressure.configure :default
@14:33:54.01 ExtraPump.configure :default
@14:33:54.11 PinPort.configure :default
@14:33:54.17 All elf controllers running firmware version 4.79
@14:33:54.20 Gate.power :motor,:ON,limit to 5000mA
@14:33:54.38 <Archive_home> Archive.in :default
@14:33:54.70 Archive.home.jog 0
@14:33:54.85 <Toroid_home> Toroid.in :default
@14:33:54.93 <Plunge_home> Plunge.in :default
@14:33:54.96 <Lysis1_home> Lysis1.in :default
@14:33:55.02 <PinPort_home> PinPort.in :default
@14:33:55.08 <Air_home> Air.in :default
@14:33:55.13 <Lysis2_home> Lysis2.in :default
@14:33:55.30 <Toroid_home> Toroid.home.jog 0
@14:33:55.33 <Plunge_home> Plunge.home.jog 0
@14:33:55.39 <Lysis1_home> Lysis1.home.jog 0
@14:33:55.41 <PinPort_home> PinPort.home.jog 0
@14:33:55.45 <Lysis2_home> Lysis2.home.jog 0
@14:33:55.49 <Air_home> Air.home.jog 0
@14:33:55.81 <Archive_home> Archive.in :homing
@14:33:56.00 <PinPort_home> PinPort.in :homing
@14:33:56.02 <Toroid_home> Toroid.in :homing
@14:33:56.13 <Archive_home> Archive.jog 155
@14:33:56.16 <Plunge_home> Plunge.in :homing
@14:33:56.32 <PinPort_home> PinPort.jog -104
@14:33:56.35 <Toroid_home> Toroid.jog 658
@14:33:56.38 <Plunge_home> Plunge.jog 1555
@14:33:56.64 <Air_home> Air.in :homing
@14:33:56.66 <Lysis2_home> Lysis2.in :homing
@14:33:56.71 <Lysis1_home> Lysis1.in :homing
@14:33:56.92 <Air_home> Air.jog 155
@14:33:57.00 <Lysis1_home> Lysis1.jog 1380
@14:33:57.10 <Lysis2_home> Lysis2.jog 1403
@14:33:57.43 <PinPort_home> PinPort.setRawPosition! 0
@14:33:57.48 <Archive_home> Archive.setRawPosition! 50
@14:33:57.70 <PinPort_home> PinPort.seek :home
@14:33:57.75 <Archive_home> Archive.seek :home
@14:33:58.16 <Air_home> Air.setRawPosition! 50
@14:33:58.23 <Plunge_home> Plunge.setRawPosition! 50
@14:33:58.29 <PinPort_home> PinPort.in :default
@14:33:58.42 <Air_home> Air.seek :home
@14:33:58.49 <Plunge_home> Plunge.seek :home
@14:33:58.64 <Lysis2_home> Lysis2.setRawPosition! 50
@14:33:58.66 <Lysis1_home> Lysis1.setRawPosition! 50
@14:33:58.68 <Archive_home> Archive.in :default
@14:33:58.81 <Lysis2_home> Lysis2.seek :home
@14:33:58.85 <Lysis1_home> Lysis1.seek :home
@14:33:59.01 <Toroid_home> Toroid.setRawPosition! 0
@14:33:59.04 <Air_home> Air.in :default
@14:33:59.06 <Plunge_home> Plunge.in :default
@14:33:59.25 <Toroid_home> Toroid.seek :home
@14:33:59.37 Toroid.in :default
@14:33:59.40 <Lysis2_home> Lysis2.in :default
@14:33:59.43 <Lysis1_home> Lysis1.in :default
@14:33:59.90 <LRAUV> Toroid.seek :clear
@14:34:01.56 Carousel.in :default
@14:34:01.63 Carousel.home.jog 0
@14:34:02.11 Carousel.in :default
@14:34:02.17 Carousel.seek 49
@14:34:02.33 Selecting Cartridge 48
@14:34:02.60 Carousel.seek 48
@14:34:03.08 Lysis1.in :fast
@14:34:03.14 Lysis1.seek :locked
@14:34:05.75 <Plunge> Plunge.seek :locked
@14:34:05.89 <Air> Air.in :fast
@14:34:05.96 Air.seek :locked
@14:34:08.42 <LRAUV> Powering cartridge bus
@14:34:11.01 Gate.setIR 1750,5000,0,6
@14:34:11.13 Gate.setIR 0,5000,0,6
@14:34:11.20 PuckHeater.configure :default
@14:34:11.26 Gate.power :heat,:ON,limit to 5000mA
@14:34:11.93 Mounted lysis Cartridge board#Q05065
@14:34:11.97 Using default puck heat loss curve for HeatingAccelInCarousel
@14:34:30.31 -> Cmd.startFiltering
@14:34:30.55 <FILTERING> Preparing to filter cartridge 48
@14:34:30.82 Priming sample loop w/100ml, bypass w/10ml
@14:34:30.84 SamplePump.setPosition! 0ml
@14:34:30.89 Bypass.open
@14:34:32.36 Intake.open
@14:34:33.09 Sea 7.8m deep (26.2psia)
@14:34:33.11 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@14:34:35.70 Vacuum tare is 54% of full scale
@14:34:35.83 Pressure.in :default
@14:34:36.28 Pressure.hold
@14:34:36.46 SamplePump.in :prime
@14:34:36.53 SamplePump.seek 10ml,59 seconds
@14:34:45.15 Pressure.stop
@14:34:45.20 SamplePump.setPosition! 0ml
@14:34:45.25 Exhaust.open
@14:34:46.70 <closeBypass> Bypass.close
@14:34:48.20 <FILTERING> Pressure.in :default
@14:34:48.26 Pressure.hold
@14:34:48.38 Sea 7.9m deep (26.3psia)
@14:34:48.52 SamplePump.in :prime
@14:34:48.60 SamplePump.seek 100ml,5:16
@14:36:23.76 Pressure.stop
@14:36:23.82 Bypass.open
@14:36:24.49 Intake.close
@14:36:25.25 Toroid.in :hold
@14:36:25.32 Toroid.seek :crack
@14:36:26.83 Toroid.seek :cartridge
@14:36:27.92 Toroid.in :default
@14:36:27.98 Toroid.seek :clear
@14:36:29.95 Pressurizing cartridge to 26psi above 26.4psia
@14:36:29.96 SamplePump.setPosition! 0ml
@14:36:30.01 Intake.open
@14:36:30.67 Bypass.close
@14:36:31.36 Exhaust.close
@14:36:32.15 Pressure.in :default
@14:36:32.22 Pressure.hold
@14:36:32.40 SamplePump.in :charge
@14:36:32.46 SamplePump.seek 2.3ml,42 seconds
@14:36:41.83 Pressure.stop
@14:36:41.88 Intake.close
@14:36:42.57 Pressurized cartridge with 0.420ml to 26.7psi
@14:36:42.58 Isolated @50.4psia (24.1psi)
@14:36:42.59 Verifying cartridge holds at least 0.65..0.85 of 50.4psia
@14:36:42.62 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@14:36:47.27 Sampling 1000.0ml
@14:36:47.28 SamplePump.setPosition! 0ml
@14:36:47.34 Exhaust.open
@14:36:48.05 Intake.open
@14:36:48.82 Pressure.in :default
@14:36:48.88 Pressure.hold
@14:36:49.00 Sea 7.7m deep (26.1psia)
@14:36:49.13 SamplePump.in :sample
@14:36:49.21 SamplePump.seek 1000ml,1:23:48
@14:36:51.85 <sampling> Cartridge 48 filtering 1/1000ml at 0.38ml/s with 22.2psi vacuum 5.8m deep (23.3psia)
@14:37:51.85 Cartridge 48 filtering 24/1000ml at 0.40ml/s with 24.0psi vacuum 7.1m deep (25.1psia)
@14:38:51.85 Cartridge 48 filtering 47/1000ml at 0.35ml/s with 21.1psi vacuum 6.9m deep (24.9psia)
@14:39:51.85 Cartridge 48 filtering 68/1000ml at 0.36ml/s with 25.2psi vacuum 7.6m deep (25.9psia)
@14:40:51.85 Cartridge 48 filtering 88/1000ml at 0.36ml/s with 25.2psi vacuum 6.8m deep (24.8psia)
@14:41:51.85 Cartridge 48 filtering 108/1000ml at 0.33ml/s with 21.3psi vacuum 7.2m deep (25.4psia)
@14:42:51.86 Cartridge 48 filtering 127/1000ml at 0.29ml/s with 21.7psi vacuum 8.3m deep (27.0psia)
@14:43:51.87 Cartridge 48 filtering 145/1000ml at 0.31ml/s with 20.3psi vacuum 7.6m deep (25.9psia)
@14:44:51.86 Cartridge 48 filtering 163/1000ml at 0.27ml/s with 23.7psi vacuum 7.7m deep (26.1psia)
@14:45:51.85 Cartridge 48 filtering 180/1000ml at 0.31ml/s with 21.2psi vacuum 7.5m deep (25.8psia)
@14:46:51.86 Cartridge 48 filtering 198/1000ml at 0.30ml/s with 25.2psi vacuum 6.0m deep (23.5psia)
@14:47:51.85 Cartridge 48 filtering 214/1000ml at 0.30ml/s with 25.6psi vacuum 6.8m deep (24.8psia)
@14:48:51.85 Cartridge 48 filtering 231/1000ml at 0.29ml/s with 22.5psi vacuum 6.8m deep (24.8psia)
@14:49:51.85 Cartridge 48 filtering 247/1000ml at 0.26ml/s with 22.9psi vacuum 7.3m deep (25.4psia)
@14:50:51.86 Cartridge 48 filtering 262/1000ml at 0.23ml/s with 22.9psi vacuum 6.3m deep (24.0psia)
@14:51:51.86 Cartridge 48 filtering 278/1000ml at 0.25ml/s with 25.7psi vacuum 7.1m deep (25.2psia)
@14:52:51.85 Cartridge 48 filtering 293/1000ml at 0.24ml/s with 26.0psi vacuum 6.1m deep (23.7psia)
@14:53:51.85 Cartridge 48 filtering 308/1000ml at 0.23ml/s with 24.9psi vacuum 5.2m deep (22.4psia)
@14:54:51.85 Cartridge 48 filtering 322/1000ml at 0.24ml/s with 23.3psi vacuum 5.5m deep (22.9psia)
@14:55:51.85 Cartridge 48 filtering 336/1000ml at 0.24ml/s with 25.6psi vacuum 5.9m deep (23.5psia)
@14:56:51.85 Cartridge 48 filtering 350/1000ml at 0.26ml/s with 23.7psi vacuum 6.6m deep (24.4psia)
@14:57:51.85 Cartridge 48 filtering 364/1000ml at 0.27ml/s with 25.8psi vacuum 6.8m deep (24.8psia)
@14:58:51.90 Cartridge 48 filtering 378/1000ml at 0.20ml/s with 21.8psi vacuum 7.6m deep (25.9psia)
@14:59:51.85 Cartridge 48 filtering 391/1000ml at 0.22ml/s with 24.2psi vacuum 5.8m deep (23.2psia)
@15:00:51.85 Cartridge 48 filtering 405/1000ml at 0.20ml/s with 23.1psi vacuum 5.3m deep (22.6psia)
@15:01:51.85 Cartridge 48 filtering 418/1000ml at 0.20ml/s with 26.2psi vacuum 4.5m deep (21.3psia)
@15:02:51.86 Cartridge 48 filtering 431/1000ml at 0.23ml/s with 21.3psi vacuum 7.0m deep (25.0psia)
@15:03:51.85 Cartridge 48 filtering 443/1000ml at 0.22ml/s with 22.5psi vacuum 5.0m deep (22.1psia)
@15:04:51.85 Cartridge 48 filtering 456/1000ml at 0.20ml/s with 25.3psi vacuum 6.8m deep (24.7psia)
@15:05:51.85 Cartridge 48 filtering 468/1000ml at 0.20ml/s with 24.4psi vacuum 5.3m deep (22.6psia)
@15:06:51.85 Cartridge 48 filtering 481/1000ml at 0.20ml/s with 24.5psi vacuum 7.0m deep (25.1psia)
@15:07:51.85 Cartridge 48 filtering 493/1000ml at 0.22ml/s with 23.9psi vacuum 6.3m deep (24.0psia)
@15:08:51.86 Cartridge 48 filtering 506/1000ml at 0.20ml/s with 25.0psi vacuum 6.6m deep (24.4psia)
@15:09:51.85 Cartridge 48 filtering 518/1000ml at 0.20ml/s with 27.9psi vacuum 5.8m deep (23.3psia)
@15:10:51.85 Cartridge 48 filtering 530/1000ml at 0.20ml/s with 25.0psi vacuum 5.5m deep (22.8psia)
@15:11:27.42 <FILTERING> Flow averaged 0.26ml/s over 34:39
@15:11:27.46 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi
@15:11:28.03 Relieved excess vacuum in .6 seconds
@15:11:28.05 SamplePump.seek 1000ml,39:04
@15:11:51.86 <sampling> Cartridge 48 filtering 542/1000ml at 0.20ml/s with 24.9psi vacuum 5.1m deep (22.2psia)
@15:12:51.85 Cartridge 48 filtering 554/1000ml at 0.20ml/s with 27.9psi vacuum 5.2m deep (22.4psia)
@15:13:03.69 <FILTERING> Flow averaged 0.20ml/s over 1:36
@15:13:03.72 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi
@15:13:04.30 Relieved excess vacuum in .6 seconds
@15:13:04.32 SamplePump.seek 1000ml,37:27
@15:13:51.86 <sampling> Cartridge 48 filtering 566/1000ml at 0.20ml/s with 23.0psi vacuum 5.7m deep (23.1psia)
@15:14:04.37 <FILTERING> Flow averaged 0.20ml/s over 1:01
@15:14:04.41 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi
@15:14:04.98 Relieved excess vacuum in .6 seconds
@15:14:05.00 SamplePump.seek 1000ml,36:26
@15:14:51.86 <sampling> Cartridge 48 filtering 578/1000ml at 0.20ml/s with 28.4psi vacuum 5.2m deep (22.4psia)
@15:15:16.47 <FILTERING> Flow averaged 0.20ml/s over 1:12
@15:15:16.51 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi
@15:15:17.09 Relieved excess vacuum in .6 seconds
@15:15:17.11 SamplePump.seek 1000ml,35:14
@15:15:51.86 <sampling> Cartridge 48 filtering 590/1000ml at 0.20ml/s with 23.3psi vacuum 5.0m deep (22.1psia)
@15:16:02.71 <FILTERING> Flow averaged 0.20ml/s over 46 seconds
@15:16:02.74 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi
@15:16:03.32 Relieved excess vacuum in .6 seconds
@15:16:03.34 SamplePump.seek 1000ml,34:28
@15:16:18.26 Flow averaged 0.20ml/s over 15 seconds
@15:16:18.28 <closeIntake> Intake.close
@15:16:18.33 <closeExhaust> Exhaust.close
@15:16:18.52 <FILTERING> Toroid.in :hold
@15:16:18.59 Toroid.seek :bypass
@15:16:20.63 Toroid.in :default
@15:16:20.69 Toroid.seek :clear
@15:16:22.30 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@15:16:22.31 Pressure.stop
@15:16:22.50 Sampled  595.2ml
@15:16:24.50 <LRAUV> -> Cmd.startProcessing
@15:16:25.94 <PROCESSING> SPR.command \"read_sequences\"
@15:16:26.06 SPR.command \"stats_on\"
@15:16:26.17 SPR.command \"start 0 1\"
@15:16:26.91 <SPRlogger> SPR.start /var/log/esp/spr22may2015h.spr.bz2
@15:16:28.91 <PROCESSING> Handoff.select :waste
@15:16:35.59 Syringe tare is 18% of full scale
@15:16:35.62 PinPort.in :locking
@15:16:35.69 PinPort.seek :locked
@15:16:36.17 <posArchive> Archive.in :fast
@15:16:36.21 <posLyS2> Lysis2.in :fast
@15:16:36.27 <PROCESSING> Lysis1.seek 1350uL
@15:16:36.41 <posArchive> Archive.seek :full
@15:16:36.45 <posLyS2> Lysis2.seek :full
@15:16:38.41 <PROCESSING> Plunge.seek :reagent
@15:16:39.28 Evacuating puck with filter bubble point of 50psi
@15:16:39.30 PinPort.seek :locked
@15:16:39.39 Handoff.select :waste
@15:16:45.97 Air syringe pumping up to 1 stroke to reach 8psi
@15:16:45.98 Air stroke #1 of 1
@15:16:46.07 Air.in :pressurize
@15:16:46.14 Air.seek :pressurized
@15:16:57.76 1.5psi after pressurize
@15:16:57.80 Air.in :compress
@15:16:57.88 Air.seek :evac
@15:17:01.96 1.3psi after compress
@15:17:14.53 0.1psi after compress (stable)
@15:17:14.54 Air.in :default
@15:17:14.61 Air.hold
@15:17:14.81 at 0.0psi Opened to waste
@15:17:14.82 Handoff.select :blocked
@15:17:14.96 Archive syringe pumping up to 8 strokes to reach 50psi
@15:17:14.97 Archive stroke #1 of 8
@15:17:15.05 Archive.in :pressurize
@15:17:15.12 Archive.seek :pressurized
@15:17:27.98 5.2psi after pressurize
@15:17:28.02 Archive.in :compress
@15:17:28.11 Archive.seek :empty
@15:17:42.79 26.3psi after compress
@15:17:57.39 30.7psi after compress (stable)
@15:17:57.42 Archive.in :gulp
@15:17:57.49 Archive.seek :topVent
@15:18:04.65 31.0psi after gulp
@15:18:17.25 30.9psi after gulp (stable)
@15:18:17.27 Archive stroke #2 of 8
@15:18:17.36 Archive.in :pressurize
@15:18:17.43 Archive.seek :pressurized
@15:18:30.27 30.8psi after pressurize
@15:18:30.29 Archive.in :compress
@15:18:30.37 Archive.seek :empty
@15:18:45.06 47.1psi after compress
@15:18:57.63 49.4psi after compress (stable)
@15:18:57.65 Archive.in :gulp
@15:18:57.72 Archive.seek :topVent
@15:19:04.89 48.4psi after gulp
@15:19:17.47 47.5psi after gulp (stable)
@15:19:17.48 Archive stroke #3 of 8
@15:19:17.57 Archive.in :pressurize
@15:19:17.64 Archive.seek :pressurized
@15:19:30.48 47.0psi after pressurize
@15:19:30.49 Archive.in :compress
@15:19:30.57 Archive.seek :empty
@15:19:39.13 Reached 50.2 of 50.0psi after pumping 5058uL (2.92 strokes)
@15:19:39.14 Archive.in :default
@15:19:39.21 Archive.hold
@15:19:39.28 Handoff.select :waste
@15:19:46.47 delay 1:00
@15:20:46.48 Handoff.select :blocked
@15:21:09.15 Handoff.select :waste
@15:21:09.27 2.8psi at evacEnd
@15:21:09.28 Handoff.select :blocked
@15:21:09.43 Archive.in :fast
@15:21:09.50 Archive.seek :topVent
@15:21:22.70 Stable @1.6psi after Handoff blocked
@15:21:22.72 Archive syringe pumping up to 8 strokes to reach 32psi
@15:21:22.74 Archive stroke #1 of 8
@15:21:22.83 Archive.in :pressurize
@15:21:22.90 Archive.seek :pressurized
@15:21:35.75 2.7psi after pressurize
@15:21:36.13 Archive.in :compress
@15:21:36.22 Archive.seek :empty
@15:21:50.91 7.1psi after compress
@15:22:19.48 16.8psi after compress (stable)
@15:22:19.50 Archive.in :gulp
@15:22:19.57 Archive.seek :topVent
@15:22:26.74 17.7psi after gulp
@15:22:39.30 18.4psi after gulp (stable)
@15:22:39.31 Archive stroke #2 of 8
@15:22:39.41 Archive.in :pressurize
@15:22:39.48 Archive.seek :pressurized
@15:22:52.32 19.0psi after pressurize
@15:22:52.34 Archive.in :compress
@15:22:52.43 Archive.seek :empty
@15:23:07.13 22.2psi after compress
@15:23:34.70 31.1psi after compress (stable)
@15:23:34.72 Archive.in :gulp
@15:23:34.80 Archive.seek :topVent
@15:23:36.10 Reached 31.7 of 31.6psi after pumping 3460uL (2.00 strokes)
@15:23:36.11 Archive.in :default
@15:23:36.18 Archive.hold
@15:23:37.02 Handoff.select :waste
@15:23:44.20 delay 1:00
@15:24:44.21 Handoff.select :blocked
@15:25:06.92 Handoff.select :waste
@15:25:07.04 2.5psi at evacEnd
@15:25:07.06 Air.seek :fullVent
@15:25:08.48 Archive.in :fast
@15:25:08.55 Archive.seek :topVent
@15:25:14.55 Seawater evacuated, Presure above/below filter released. Lysis begins
@15:25:14.64 Lysis1.in :slow
@15:25:14.71 Lysis1.seek 1050uL
@15:25:44.72 delay 5 seconds
@15:25:49.74 Handoff.select :blocked
@15:25:49.91 Lysis1.seek 450uL
@15:26:49.86 delay 3 seconds
@15:26:52.94 Air.in mix with 1.40048 parts Lysis1
@15:26:53.39 <Airmixer> Air.seek :evac
@15:26:53.43 <PROCESSING> Lysis1.seek 300uL
@15:27:08.54 Lysis1.seek :empty
@15:27:38.63 Lysis buffer delivered @16.8psi
@15:27:38.64 Polling Can every 2:00
@15:27:39.19 PuckHeater.configure :default #ambient 12.7C
@15:27:39.24 PuckHeater.seek 80.2C
@15:28:36.99 PuckHeater.hold 2:00,80.2C
@15:30:36.73 Polling Can every 2:00
@15:30:37.27 PuckHeater.configure :default #ambient 12.8C
@15:30:37.32 PuckHeater.hold 30 seconds,79.1C
@15:31:07.42 Polling Can every 2:00
@15:31:07.96 PuckHeater.configure :default #ambient 12.8C
@15:31:08.02 PuckHeater.hold 30 seconds,78.6C
@15:31:38.17 Polling Can every 2:00
@15:31:38.71 PuckHeater.configure :default #ambient 12.7C
@15:31:38.76 PuckHeater.hold 10:00,78.1C
@15:41:37.34 Lysis complete. Syringe pressure @21.0psi
@15:41:37.35 Handoff.select :blocked
@15:41:37.40 Archive syringe pumping up to 5 strokes to reach 40psi
@15:41:37.41 Archive stroke #1 of 5
@15:41:37.50 Archive.in :pressurize
@15:41:37.57 Archive.seek :pressurized
@15:41:50.46 20.6psi after pressurize
@15:41:50.48 Archive.in :compress
@15:41:50.56 Archive.seek :empty
@15:41:59.30 Reached 40.6 of 40.0psi after pumping 1602uL (0.93 strokes)
@15:41:59.31 Archive.in :default
@15:41:59.38 Archive.hold
@15:41:59.45 delay 2 seconds
@15:42:01.47 Archive.in :fast
@15:42:01.53 Archive.seek :full
@15:42:08.58 Lysis1.seek 138uL
@15:42:22.45 delay 1 second
@15:42:23.49 <fillArchive> Archive.seek :full
@15:42:23.53 <PROCESSING> Lysis1.seek :empty
@15:42:37.51 Initial air pressure @44.3psi
@15:42:37.58 Lysis1.in :extract
@15:42:37.71 Lysis1.seek 600uL,nil
@15:42:41.04 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@15:42:41.05 Archive stroke #1 of 6
@15:42:41.20 Archive.in :pressurize
@15:42:41.26 Archive.seek :pressurized
@15:42:54.13 15.8psi after pressurize
@15:42:54.15 Archive.in :compress
@15:42:54.23 Archive.seek :empty
@15:43:03.11 Syringe::LysatePlungerStuck in PROCESSING -- at < 338uL during Lysis Syringe #1 (LyS1) move
@15:43:03.15 <PROCESSING> Lysis1.in :default
@15:43:03.22 Lysis1.seek 303uL
@15:43:03.87 <pump> Reached 27.5 of 25.0psi after pumping 1622uL (0.94 strokes)
@15:43:03.89 Archive.in :default
@15:43:03.96 Archive.hold
@15:43:05.08 <PROCESSING> Lysis1.in :extract
@15:43:05.17 Lysis1.seek 600uL,nil
@15:43:09.37 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@15:43:09.38 Archive stroke #1 of 6
@15:43:09.53 Archive.in :compress
@15:43:09.59 Archive.seek :empty
@15:43:10.87 Archive.in :default
@15:43:10.92 Archive.hold
@15:43:11.07 <PROCESSING> Initial 600uL lysate @17.3psi
@15:43:11.08 Handoff.select :blocked
@15:43:11.18 Initial air pressure @18.2psi
@15:43:11.26 Lysis1.seek 825uL,nil
@15:43:12.45 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@15:43:12.46 Archive stroke #1 of 6
@15:43:12.57 Archive.in :compress
@15:43:12.67 Archive.seek :empty
@15:43:16.74 16.1psi after compress
@15:43:29.30 15.5psi after compress (stable)
@15:43:34.48 Archive.in :gulp
@15:43:34.54 Archive.seek :topVent
@15:43:41.70 15.6psi after gulp
@15:43:54.26 15.6psi after gulp (stable)
@15:43:54.27 Archive stroke #2 of 6
@15:43:54.37 Archive.in :pressurize
@15:43:54.43 Archive.seek :pressurized
@15:44:07.32 15.4psi after pressurize
@15:44:07.33 Archive.in :compress
@15:44:07.42 Archive.seek :empty
@15:44:17.89 Archive.in :default
@15:44:17.94 Archive.hold
@15:44:18.17 <PROCESSING> Recovered 825uL lysate @15.9psi
@15:44:18.18 Air.in :halfSpeed
@15:44:18.25 Air.seek :fullVent
@15:44:20.46 Puck Top Vented, bottom @4.7psi
@15:44:20.47 Handoff.select :waste
@15:44:20.59 delay 5 seconds
@15:44:25.65 Handoff open to waste, bottom @0.1psi
@15:44:25.67 Air.in :default
@15:44:25.74 Air.seek :evac
@15:44:27.15 Lysis2.in :DAmix
@15:44:27.30 Lysis1.in :DAmix
@15:44:27.82 <Lysis2mixer> Lysis2.seek 1480uL
@15:44:27.88 <PROCESSING> Lysis1.seek 575uL
@15:45:02.92 delay 5 seconds
@15:45:07.96 Handoff.select SPR
@15:45:08.09 SPR.command \"start 2 14\"
@15:45:09.86 SPR stabilized
@15:45:10.00 delay 10 seconds
@15:45:20.02 SPR.command \"start 1 0\"
@15:45:22.74 Waiting up to 4:00 for SPR to enter state 1
@15:47:25.10 SPR.command \"hold_on\"
@15:47:26.52 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@15:47:27.04 <Lysis2mixer> Lysis2.seek 1180uL
@15:47:27.08 <PROCESSING> Lysis1.seek 275uL
@15:48:09.09 delay 15 seconds
@15:48:24.11 SPR.command \"hold_off\"
@15:48:27.18 Waiting up to 38:00 for SPR to enter state 7
@16:09:17.01 <SPRlogger> SPRsummary:-7.59467,96.7533,101.924,100.773 after 3324s
@16:15:54.23 <PROCESSING> SPR analysis completed
@16:16:00.41 <SPRlogger> Ended log spr22may2015h.spr.bz2
@16:16:00.43 <PROCESSING> SPR.command \"stats_off\"
@16:16:02.00 <LRAUV> -> Cmd.stop
@16:16:02.09 <closeHandoff> Handoff.select :close
@16:16:02.23 <LRAUV> Gate.power :heat,:OFF
@16:16:02.24 Gate.power :cartridge,:OFF
@16:16:02.33 Carousel.seek 48
@16:16:02.51 Plunge.seek :locked
@16:16:03.37 PinPort.in :unlocking
@16:16:03.43 PinPort.seek :clear
@16:16:03.96 <Archive> Archive.in :unlocking
@16:16:04.09 Archive.seek :clear
@16:16:04.18 <Air> Air.in :unlocking
@16:16:04.30 Air.seek :clear
@16:16:04.33 <Lysis1> Lysis1.in :unlocking
@16:16:04.52 <Lysis2> Lysis2.in :unlocking
@16:16:04.56 <Lysis1> Lysis1.seek :clear
@16:16:04.66 <Lysis2> Lysis2.seek :clear
@16:16:08.24 <LRAUV> Gate.power :motor,:OFF
@16:16:08.26 Gate.power :core,:OFF
@16:16:08.28 Gate.power :analytic1,:OFF
@16:16:08.30 Gate.power :analytic2,:OFF
@16:16:08.33 Gate.power :analytic3,:OFF
@16:16:08.36 Gate.power :main,:OFF
@16:16:08.38 Safely stopped and ready to power off
##-- file closed 2022-05-21T21:31:51.718Z

##-- file opened 2022-05-21T21:31:51.886Z
@14:31:51.63 -> showStatus
@14:31:53.65 -> Cmd.loadCartridge -10
@14:31:53.71 Gate.power :main,:ON,limit to 5000mA
@14:31:53.74 Gate.power :analytic1,:ON,limit to 5000mA
@14:31:53.76 Gate.power :analytic2,:ON,limit to 5000mA
@14:31:53.78 Gate.power :analytic3,:ON,limit to 5000mA
@14:31:53.93 Powering core bus
@14:31:56.19 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@14:31:56.64 <LRAUV> Archive.configure :default
@14:31:56.77 Air.configure :default
@14:31:56.90 Lysis1.configure :default
@14:31:57.10 Lysis2.configure :default
@14:31:57.36 Plunge.configure :default
@14:31:57.65 SamplePump.configure :default
@14:31:57.94 Toroid.configure :default
@14:31:58.24 Carousel.configure :default
@14:31:58.51 Pressure.configure :default
@14:31:58.63 ExtraPump.configure :default
@14:31:58.74 PinPort.configure :default
@14:31:58.80 All elf controllers running firmware version 4.79
@14:31:58.82 Gate.power :motor,:ON,limit to 5000mA
@14:31:59.00 <Archive_home> Archive.in :default
@14:31:59.25 Archive.home.jog 0
@14:31:59.45 <Toroid_home> Toroid.in :default
@14:31:59.50 <Plunge_home> Plunge.in :default
@14:31:59.53 <Lysis1_home> Lysis1.in :default
@14:31:59.59 <Air_home> Air.in :default
@14:31:59.64 <Lysis2_home> Lysis2.in :default
@14:31:59.68 <PinPort_home> PinPort.in :default
@14:31:59.86 <Toroid_home> Toroid.home.jog 0
@14:31:59.97 <Plunge_home> Plunge.home.jog 0
@14:32:00.00 <Lysis1_home> Lysis1.home.jog 0
@14:32:00.02 <Lysis2_home> Lysis2.home.jog 0
@14:32:00.05 <Air_home> Air.home.jog 0
@14:32:00.09 <PinPort_home> PinPort.home.jog 0
@14:32:00.41 <Archive_home> Archive.in :homing
@14:32:00.47 Archive.jog 1399
@14:32:00.63 <Plunge_home> Plunge.in :homing
@14:32:00.74 <Toroid_home> Toroid.in :homing
@14:32:00.86 Toroid.jog 652
@14:32:00.89 <Plunge_home> Plunge.jog 1562
@14:32:01.05 <Lysis1_home> Lysis1.in :homing
@14:32:01.10 <PinPort_home> PinPort.in :homing
@14:32:01.13 <Lysis2_home> Lysis2.in :homing
@14:32:01.16 <Air_home> Air.in :homing
@14:32:01.31 <Lysis1_home> Lysis1.jog 1382
@14:32:01.41 <PinPort_home> PinPort.jog -104
@14:32:01.43 <Air_home> Air.jog 1392
@14:32:01.47 <Lysis2_home> Lysis2.jog 1403
@14:32:01.62 <Archive_home> Archive.setRawPosition! 50
@14:32:01.67 Archive.seek :home
@14:32:01.76 <PinPort_home> PinPort.setRawPosition! 0
@14:32:01.81 PinPort.seek :home
@14:32:01.89 PinPort.in :default
@14:32:01.98 <Archive_home> Archive.in :default
@14:32:02.14 <Plunge_home> Plunge.setRawPosition! 50
@14:32:02.19 Plunge.seek :home
@14:32:02.46 Plunge.in :default
@14:32:02.55 <Lysis1_home> Lysis1.setRawPosition! 50
@14:32:02.60 Lysis1.seek :home
@14:32:02.70 <Lysis2_home> Lysis2.setRawPosition! 50
@14:32:02.72 <Air_home> Air.setRawPosition! 50
@14:32:02.79 <Lysis2_home> Lysis2.seek :home
@14:32:02.85 <Air_home> Air.seek :home
@14:32:02.98 <Lysis1_home> Lysis1.in :default
@14:32:03.10 <Lysis2_home> Lysis2.in :default
@14:32:03.19 <Toroid_home> Toroid.setRawPosition! 0
@14:32:03.22 <Air_home> Air.in :default
@14:32:03.29 <Toroid_home> Toroid.seek :home
@14:32:03.42 Toroid.in :default
@14:32:03.71 <LRAUV> Toroid.seek :clear
@14:32:05.32 Carousel.in :default
@14:32:05.38 Carousel.home.jog 0
@14:32:05.87 Carousel.in :default
@14:32:05.93 Carousel.seek 48
@14:32:06.09 Selecting Cartridge 47
@14:32:06.36 Carousel.seek 47
@14:32:06.84 Lysis1.in :fast
@14:32:06.90 Lysis1.seek :locked
@14:32:09.50 <Plunge> Plunge.seek :locked
@14:32:09.64 <Air> Air.in :fast
@14:32:09.70 Air.seek :locked
@14:32:12.16 <LRAUV> Powering cartridge bus
@14:32:14.76 Gate.setIR 1750,5000,0,6
@14:32:14.88 Gate.setIR 0,5000,0,6
@14:32:14.94 PuckHeater.configure :default
@14:32:15.00 Gate.power :heat,:ON,limit to 5000mA
@14:32:15.70 Mounted lysis Cartridge board#Q05057
@14:32:15.74 Using default puck heat loss curve for HeatingAccelInCarousel
@14:32:34.05 -> Cmd.startFiltering
92.07@14:32:34.30 <FILTERING> Preparing to filter cartridge 47
@14:32:34.58 Priming sample loop w/100ml, bypass w/10ml
@14:32:34.60 SamplePump.setPosition! 0ml
@14:32:34.65 Bypass.open
@14:32:36.08 Intake.open
@14:32:36.80 Sea 8.0m deep (26.5psia)
@14:32:36.82 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@14:32:39.40 Vacuum tare is 54% of full scale
@14:32:39.93 Pressure.in :default
@14:32:39.99 Pressure.hold
@14:32:40.17 SamplePump.in :prime
@14:32:40.24 SamplePump.seek 10ml,59 seconds
@14:32:48.86 Pressure.stop
@14:32:48.92 SamplePump.setPosition! 0ml
@14:32:48.97 Exhaust.open
@14:32:50.40 <closeBypass> Bypass.close
@14:32:51.90 <FILTERING> Pressure.hold
@14:32:52.02 Sea 7.9m deep (26.4psia)
@14:32:52.16 SamplePump.in :prime
@14:32:52.23 SamplePump.seek 100ml,5:16
@14:34:27.62 Pressure.stop
@14:34:27.68 Bypass.open
@14:34:28.38 Intake.close
@14:34:29.09 Toroid.in :hold
@14:34:29.15 Toroid.seek :crack
@14:34:30.66 Toroid.seek :cartridge
@14:34:31.75 Toroid.in :default
@14:34:31.81 Toroid.seek :clear
@14:34:33.77 Pressurizing cartridge to 26psi above 26.7psia
@14:34:33.78 SamplePump.setPosition! 0ml
@14:34:33.83 Intake.open
@14:34:34.51 Bypass.close
@14:34:35.21 Exhaust.close
@14:34:36.03 Pressure.in :default
@14:34:36.08 Pressure.hold
@14:34:36.26 SamplePump.in :charge
@14:34:36.33 SamplePump.seek 2.3ml,42 seconds
@14:34:49.58 Pressure.stop
@14:34:49.64 Intake.close
@14:34:50.38 Pressurized cartridge with 0.848ml to 26.1psi
@14:34:50.39 Isolated @43.8psia (17.1psi)
@14:34:50.40 Verifying cartridge holds at least 0.65..0.85 of 43.8psia
@14:34:50.44 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@14:34:55.06 Sampling 1000.0ml
@14:34:55.08 SamplePump.setPosition! 0ml
@14:34:55.14 Exhaust.open
@14:34:55.84 Intake.open
@14:34:56.64 Pressure.in :default
@14:34:56.70 Pressure.hold
@14:34:56.82 Sea 11.8m deep (32.2psia)
@14:34:56.96 SamplePump.in :sample
@14:34:57.03 SamplePump.seek 1000ml,1:23:48
@14:34:59.67 <sampling> Cartridge 47 filtering 1/1000ml at 0.47ml/s with 22.7psi vacuum 9.3m deep (28.5psia)
@14:35:59.67 Cartridge 47 filtering 24/1000ml at 0.36ml/s with 26.3psi vacuum 6.6m deep (24.5psia)
@14:36:59.67 Cartridge 47 filtering 46/1000ml at 0.37ml/s with 21.6psi vacuum 7.2m deep (25.4psia)
@14:37:59.66 Cartridge 47 filtering 68/1000ml at 0.33ml/s with 23.9psi vacuum 7.0m deep (25.1psia)
@14:38:59.67 Cartridge 47 filtering 88/1000ml at 0.36ml/s with 23.6psi vacuum 8.0m deep (26.4psia)
@14:39:59.67 Cartridge 47 filtering 108/1000ml at 0.31ml/s with 23.9psi vacuum 6.9m deep (24.8psia)
@14:40:59.67 Cartridge 47 filtering 127/1000ml at 0.31ml/s with 24.0psi vacuum 5.7m deep (23.1psia)
@14:41:59.67 Cartridge 47 filtering 146/1000ml at 0.30ml/s with 24.9psi vacuum 5.9m deep (23.4psia)
@14:42:59.67 Cartridge 47 filtering 165/1000ml at 0.33ml/s with 24.2psi vacuum 7.6m deep (25.9psia)
@14:43:59.66 Cartridge 47 filtering 183/1000ml at 0.30ml/s with 26.2psi vacuum 7.2m deep (25.4psia)
@14:44:59.67 Cartridge 47 filtering 201/1000ml at 0.28ml/s with 25.2psi vacuum 5.9m deep (23.4psia)
@14:45:59.66 Cartridge 47 filtering 218/1000ml at 0.28ml/s with 24.3psi vacuum 5.5m deep (22.9psia)
@14:46:59.67 Cartridge 47 filtering 236/1000ml at 0.30ml/s with 22.6psi vacuum 6.1m deep (23.8psia)
@14:47:59.66 Cartridge 47 filtering 252/1000ml at 0.30ml/s with 24.7psi vacuum 5.0m deep (22.1psia)
@14:48:59.67 Cartridge 47 filtering 269/1000ml at 0.25ml/s with 23.1psi vacuum 5.9m deep (23.4psia)
@14:49:59.66 Cartridge 47 filtering 285/1000ml at 0.29ml/s with 21.6psi vacuum 7.0m deep (25.1psia)
@14:50:59.67 Cartridge 47 filtering 301/1000ml at 0.24ml/s with 23.4psi vacuum 5.9m deep (23.5psia)
@14:51:59.66 Cartridge 47 filtering 317/1000ml at 0.25ml/s with 25.1psi vacuum 5.2m deep (22.4psia)
@14:52:59.67 Cartridge 47 filtering 332/1000ml at 0.27ml/s with 23.3psi vacuum 5.3m deep (22.5psia)
@14:53:59.66 Cartridge 47 filtering 348/1000ml at 0.24ml/s with 22.8psi vacuum 6.3m deep (24.1psia)
@14:54:59.67 Cartridge 47 filtering 363/1000ml at 0.25ml/s with 21.4psi vacuum 5.6m deep (22.9psia)
@14:55:59.66 Cartridge 47 filtering 378/1000ml at 0.23ml/s with 22.9psi vacuum 7.0m deep (25.1psia)
@14:56:59.67 Cartridge 47 filtering 393/1000ml at 0.23ml/s with 21.7psi vacuum 5.7m deep (23.2psia)
@14:57:59.67 Cartridge 47 filtering 407/1000ml at 0.23ml/s with 26.3psi vacuum 4.0m deep (20.6psia)
@14:58:59.67 Cartridge 47 filtering 422/1000ml at 0.22ml/s with 24.5psi vacuum 5.2m deep (22.4psia)
@14:59:59.66 Cartridge 47 filtering 436/1000ml at 0.24ml/s with 24.9psi vacuum 5.4m deep (22.6psia)
@15:00:59.67 Cartridge 47 filtering 450/1000ml at 0.22ml/s with 22.2psi vacuum 6.9m deep (24.9psia)
@15:01:59.66 Cartridge 47 filtering 464/1000ml at 0.22ml/s with 25.2psi vacuum 5.8m deep (23.3psia)
@15:02:59.67 Cartridge 47 filtering 478/1000ml at 0.23ml/s with 22.5psi vacuum 4.8m deep (21.7psia)
@15:03:59.66 Cartridge 47 filtering 492/1000ml at 0.24ml/s with 21.9psi vacuum 5.2m deep (22.4psia)
@15:04:59.67 Cartridge 47 filtering 506/1000ml at 0.22ml/s with 22.6psi vacuum 5.6m deep (23.0psia)
@15:05:59.66 Cartridge 47 filtering 519/1000ml at 0.24ml/s with 23.7psi vacuum 4.5m deep (21.3psia)
@15:06:59.68 Cartridge 47 filtering 533/1000ml at 0.24ml/s with 22.6psi vacuum 6.1m deep (23.8psia)
@15:07:59.67 Cartridge 47 filtering 546/1000ml at 0.20ml/s with 23.2psi vacuum 5.5m deep (22.8psia)
@15:08:59.67 Cartridge 47 filtering 559/1000ml at 0.22ml/s with 23.4psi vacuum 5.0m deep (22.1psia)
@15:09:59.66 Cartridge 47 filtering 573/1000ml at 0.21ml/s with 22.9psi vacuum 4.7m deep (21.6psia)
@15:10:59.67 Cartridge 47 filtering 586/1000ml at 0.20ml/s with 23.4psi vacuum 5.1m deep (22.2psia)
@15:11:59.66 Cartridge 47 filtering 599/1000ml at 0.23ml/s with 22.1psi vacuum 5.7m deep (23.1psia)
@15:12:59.67 Cartridge 47 filtering 611/1000ml at 0.22ml/s with 25.0psi vacuum 5.4m deep (22.6psia)
@15:13:59.66 Cartridge 47 filtering 624/1000ml at 0.23ml/s with 23.1psi vacuum 5.0m deep (22.1psia)
@15:14:59.67 Cartridge 47 filtering 637/1000ml at 0.20ml/s with 24.1psi vacuum 5.2m deep (22.4psia)
@15:15:59.66 Cartridge 47 filtering 649/1000ml at 0.21ml/s with 25.1psi vacuum 5.2m deep (22.4psia)
@15:16:59.67 Cartridge 47 filtering 662/1000ml at 0.20ml/s with 26.8psi vacuum 4.3m deep (21.0psia)
@15:17:59.66 Cartridge 47 filtering 675/1000ml at 0.20ml/s with 25.8psi vacuum 4.4m deep (21.3psia)
@15:18:59.68 Cartridge 47 filtering 687/1000ml at 0.20ml/s with 26.8psi vacuum 3.6m deep (20.1psia)
@15:19:59.66 Cartridge 47 filtering 699/1000ml at 0.20ml/s with 24.5psi vacuum 5.1m deep (22.2psia)
@15:20:59.67 Cartridge 47 filtering 712/1000ml at 0.21ml/s with 22.7psi vacuum 5.2m deep (22.4psia)
@15:21:59.66 Cartridge 47 filtering 724/1000ml at 0.20ml/s with 26.4psi vacuum 3.9m deep (20.5psia)
@15:22:59.67 Cartridge 47 filtering 736/1000ml at 0.20ml/s with 23.8psi vacuum 4.6m deep (21.6psia)
@15:23:59.66 Cartridge 47 filtering 749/1000ml at 0.20ml/s with 25.5psi vacuum 3.9m deep (20.5psia)
@15:24:59.67 Cartridge 47 filtering 761/1000ml at 0.20ml/s with 27.5psi vacuum 4.4m deep (21.3psia)
@15:25:59.66 Cartridge 47 filtering 773/1000ml at 0.20ml/s with 26.0psi vacuum 4.4m deep (21.2psia)
@15:26:59.67 Cartridge 47 filtering 785/1000ml at 0.20ml/s with 24.9psi vacuum 4.4m deep (21.2psia)
@15:27:59.67 Cartridge 47 filtering 797/1000ml at 0.20ml/s with 23.4psi vacuum 5.6m deep (23.0psia)
@15:28:59.67 Cartridge 47 filtering 810/1000ml at 0.20ml/s with 23.9psi vacuum 4.4m deep (21.3psia)
@15:29:59.66 Cartridge 47 filtering 822/1000ml at 0.20ml/s with 24.4psi vacuum 4.3m deep (21.0psia)
@15:30:59.68 Cartridge 47 filtering 834/1000ml at 0.20ml/s with 25.8psi vacuum 5.6m deep (23.0psia)
@15:31:59.66 Cartridge 47 filtering 846/1000ml at 0.20ml/s with 27.2psi vacuum 4.5m deep (21.3psia)
@15:32:22.71 <FILTERING> Flow averaged 0.25ml/s over 57:26
@15:32:22.75 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi
@15:32:23.82 Relieved excess vacuum in 1.1 seconds
@15:32:23.85 SamplePump.seek 1000ml,12:57
@15:32:33.58 Flow averaged 0.20ml/s over 10 seconds
@15:32:33.60 <closeIntake> Intake.close
@15:32:33.66 <closeExhaust> Exhaust.close
@15:32:33.84 <FILTERING> Toroid.in :hold
@15:32:33.91 Toroid.seek :bypass
@15:32:35.53 Toroid.in :default
@15:32:35.60 Toroid.seek :clear
@15:32:37.21 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@15:32:37.22 Pressure.stop
@15:32:37.41 Sampled  852.5ml
@15:32:39.47 <LRAUV> -> Cmd.startProcessing
@15:32:40.90 <PROCESSING> SPR.command \"read_sequences\"
@15:32:41.29 SPR.command \"stats_on\"
@15:32:41.40 SPR.command \"start 0 1\"
@15:32:42.14 <SPRlogger> SPR.start /var/log/esp/spr22may2115h.spr.bz2
@15:32:44.14 <PROCESSING> Handoff.select :waste
@15:32:50.87 Syringe tare is 18% of full scale
@15:32:50.90 PinPort.in :locking
@15:32:50.97 PinPort.seek :locked
@15:32:51.46 <posArchive> Archive.in :fast
@15:32:51.51 <posLyS2> Lysis2.in :fast
@15:32:51.60 <PROCESSING> Lysis1.seek 1350uL
@15:32:51.65 <posArchive> Archive.seek :full
@15:32:51.71 <posLyS2> Lysis2.seek :full
@15:32:53.71 <PROCESSING> Plunge.seek :reagent
@15:32:54.58 Evacuating puck with filter bubble point of 50psi
@15:32:54.60 PinPort.seek :locked
@15:32:54.69 Handoff.select :waste
@15:33:01.31 Air syringe pumping up to 1 stroke to reach 8psi
@15:33:01.32 Air stroke #1 of 1
@15:33:01.42 Air.in :pressurize
@15:33:01.49 Air.seek :pressurized
@15:33:13.06 1.3psi after pressurize
@15:33:13.08 Air.in :compress
@15:33:13.16 Air.seek :evac
@15:33:17.24 0.7psi after compress
@15:33:29.81 -0.2psi after compress (stable)
@15:33:29.82 Air.in :default
@15:33:29.89 Air.hold
@15:33:30.11 at -0.1psi Opened to waste
@15:33:30.12 Handoff.select :blocked
@15:33:30.26 Archive syringe pumping up to 8 strokes to reach 50psi
@15:33:30.27 Archive stroke #1 of 8
@15:33:30.36 Archive.in :pressurize
@15:33:30.42 Archive.seek :pressurized
@15:33:43.31 -0.0psi after pressurize
@15:33:43.33 Archive.in :compress
@15:33:43.41 Archive.seek :empty
@15:33:58.09 25.0psi after compress
@15:34:10.67 28.5psi after compress (stable)
@15:34:10.70 Archive.in :gulp
@15:34:10.78 Archive.seek :topVent
@15:34:17.94 29.2psi after gulp
@15:34:30.53 29.2psi after gulp (stable)
@15:34:30.55 Archive stroke #2 of 8
@15:34:30.65 Archive.in :pressurize
@15:34:30.71 Archive.seek :pressurized
@15:34:43.57 28.9psi after pressurize
@15:34:43.58 Archive.in :compress
@15:34:43.66 Archive.seek :empty
@15:34:58.34 29.8psi after compress
@15:35:10.91 31.1psi after compress (stable)
@15:35:10.93 Archive.in :gulp
@15:35:11.00 Archive.seek :topVent
@15:35:18.16 32.7psi after gulp
@15:35:30.73 33.2psi after gulp (stable)
@15:35:30.74 Archive stroke #3 of 8
@15:35:30.83 Archive.in :pressurize
@15:35:30.90 Archive.seek :pressurized
@15:35:43.79 33.3psi after pressurize
@15:35:43.81 Archive.in :compress
@15:35:43.89 Archive.seek :empty
@15:35:58.59 35.0psi after compress
@15:36:13.16 37.4psi after compress (stable)
@15:36:13.17 Archive.in :gulp
@15:36:13.26 Archive.seek :topVent
@15:36:20.42 39.6psi after gulp
@15:36:32.99 40.4psi after gulp (stable)
@15:36:33.00 Archive stroke #4 of 8
@15:36:33.09 Archive.in :pressurize
@15:36:33.16 Archive.seek :pressurized
@15:36:45.99 41.1psi after pressurize
@15:36:46.01 Archive.in :compress
@15:36:46.09 Archive.seek :empty
@15:37:00.76 44.3psi after compress
@15:37:03.93 Reached 50.2 of 50.0psi after pumping 6920uL (4.00 strokes)
@15:37:03.94 Archive.in :default
@15:37:04.01 Archive.hold
@15:37:04.08 Handoff.select :waste
@15:37:20.26 delay 1:00
@15:38:20.28 Handoff.select :blocked
@15:38:42.96 Handoff.select :waste
@15:38:43.08 2.5psi at evacEnd
@15:38:43.09 Handoff.select :blocked
@15:38:43.23 Archive.in :fast
@15:38:43.29 Archive.seek :topVent
@15:38:56.94 Stable @1.6psi after Handoff blocked
@15:38:56.97 Archive syringe pumping up to 8 strokes to reach 32psi
@15:38:56.98 Archive stroke #1 of 8
@15:38:57.08 Archive.in :pressurize
@15:38:57.14 Archive.seek :pressurized
@15:39:09.99 2.4psi after pressurize
@15:39:10.01 Archive.in :compress
@15:39:10.09 Archive.seek :empty
@15:39:24.77 5.2psi after compress
@15:40:00.85 15.0psi after compress (stable)
@15:40:00.87 Archive.in :gulp
@15:40:00.95 Archive.seek :topVent
@15:40:08.10 16.0psi after gulp
@15:40:20.67 16.5psi after gulp (stable)
@15:40:20.68 Archive stroke #2 of 8
@15:40:20.78 Archive.in :pressurize
@15:40:20.84 Archive.seek :pressurized
@15:40:33.69 16.9psi after pressurize
@15:40:33.73 Archive.in :compress
@15:40:33.81 Archive.seek :empty
@15:40:48.49 18.5psi after compress
@15:41:25.06 27.9psi after compress (stable)
@15:41:25.08 Archive.in :gulp
@15:41:25.16 Archive.seek :topVent
@15:41:32.32 28.9psi after gulp
@15:41:44.89 29.4psi after gulp (stable)
@15:41:44.90 Archive stroke #3 of 8
@15:41:45.00 Archive.in :pressurize
@15:41:45.06 Archive.seek :pressurized
@15:41:57.99 29.9psi after pressurize
@15:41:58.02 Archive.in :compress
@15:41:58.10 Archive.seek :empty
@15:42:12.24 Reached 31.7 of 31.6psi after pumping 5179uL (2.99 strokes)
@15:42:12.26 Archive.in :default
@15:42:12.33 Archive.hold
@15:42:12.40 Handoff.select :waste
@15:42:19.57 delay 1:00
@15:43:19.58 Handoff.select :blocked
@15:44:03.26 Handoff.select :waste
@15:44:03.37 7.1psi at evacEnd
@15:44:03.39 Air.seek :fullVent
@15:44:04.82 Archive.in :fast
@15:44:04.89 Archive.seek :topVent
@15:44:11.94 Seawater evacuated, Presure above/below filter released. Lysis begins
@15:44:12.03 Lysis1.in :slow
@15:44:12.10 Lysis1.seek 1050uL
@15:44:42.11 delay 5 seconds
@15:44:47.12 Handoff.select :blocked
@15:44:47.30 Lysis1.seek 450uL
@15:45:47.24 delay 3 seconds
@15:45:50.32 Air.in mix with 1.40048 parts Lysis1
@15:45:50.81 <Airmixer> Air.seek :evac
@15:45:50.85 <PROCESSING> Lysis1.seek 300uL
@15:46:05.96 Lysis1.seek :empty
@15:46:36.07 Lysis buffer delivered @14.1psi
@15:46:36.08 Polling Can every 2:00
@15:46:36.63 PuckHeater.configure :default #ambient 12.5C
@15:46:36.68 PuckHeater.seek 80.2C
@15:47:49.25 PuckHeater.hold 2:00,80.2C
@15:49:49.57 Polling Can every 2:00
@15:49:50.11 PuckHeater.configure :default #ambient 12.6C
@15:49:50.17 PuckHeater.hold 30 seconds,79.1C
@15:50:20.41 Polling Can every 2:00
@15:50:20.95 PuckHeater.hold 30 seconds,78.6C
@15:50:51.19 Polling Can every 2:00
@15:50:51.73 PuckHeater.hold 10:00,78.1C
@16:00:52.89 Lysis complete. Syringe pressure @18.0psi
@16:00:52.92 Handoff.select :blocked
@16:00:52.97 Archive syringe pumping up to 5 strokes to reach 40psi
@16:00:52.99 Archive stroke #1 of 5
@16:00:53.08 Archive.in :pressurize
@16:00:53.16 Archive.seek :pressurized
@16:01:06.01 17.7psi after pressurize
@16:01:06.03 Archive.in :compress
@16:01:06.11 Archive.seek :empty
@16:01:15.72 Reached 40.7 of 40.0psi after pumping 1620uL (0.94 strokes)
@16:01:15.74 Archive.in :default
@16:01:15.82 Archive.hold
@16:01:15.89 delay 2 seconds
@16:01:17.91 Archive.in :fast
@16:01:17.97 Archive.seek :full
@16:01:24.75 Lysis1.seek 138uL
@16:01:38.61 delay 1 second
@16:01:39.63 <fillArchive> Archive.seek :full
@16:01:39.67 <PROCESSING> Lysis1.seek :empty
@16:01:53.63 Initial air pressure @45.0psi
@16:01:53.71 Lysis1.in :extract
@16:01:53.78 Lysis1.seek 600uL,nil
@16:01:56.57 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@16:01:56.59 Archive stroke #1 of 6
@16:01:56.68 Archive.in :pressurize
@16:01:56.76 Archive.seek :pressurized
@16:02:09.69 15.9psi after pressurize
@16:02:09.71 Archive.in :compress
@16:02:09.78 Archive.seek :empty
@16:02:23.25 Archive.in :default
@16:02:23.30 Archive.hold
@16:02:23.45 <PROCESSING> Initial 600uL lysate @17.7psi
@16:02:23.47 Handoff.select :blocked
@16:02:23.57 Initial air pressure @18.6psi
@16:02:23.64 Lysis1.seek 825uL,nil
@16:02:24.85 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@16:02:24.86 Archive stroke #1 of 6
@16:02:25.00 Archive.in :compress
@16:02:25.07 Archive.seek :empty
@16:02:26.30 16.5psi after compress
@16:02:38.91 15.8psi after compress (stable)
@16:02:44.09 Archive.in :gulp
@16:02:44.16 Archive.seek :topVent
@16:02:51.31 15.8psi after gulp
@16:03:03.88 15.8psi after gulp (stable)
@16:03:03.89 Archive stroke #2 of 6
@16:03:04.00 Archive.in :pressurize
@16:03:04.08 Archive.seek :pressurized
@16:03:16.93 15.7psi after pressurize
@16:03:16.94 Archive.in :compress
@16:03:17.01 Archive.seek :empty
@16:03:31.68 15.8psi after compress
@16:03:44.25 15.9psi after compress (stable)
@16:03:44.67 Archive.in :default
@16:03:44.72 Archive.hold
@16:03:44.91 <PROCESSING> Recovered 825uL lysate @15.7psi
@16:03:44.93 Air.in :halfSpeed
@16:03:45.00 Air.seek :fullVent
@16:03:47.21 Puck Top Vented, bottom @17.6psi
@16:03:47.22 Handoff.select :waste
@16:03:47.35 delay 5 seconds
@16:03:52.41 Handoff open to waste, bottom @1.8psi
@16:03:52.43 Air.in :default
@16:03:52.50 Air.seek :evac
@16:03:53.91 Lysis2.in :DAmix
@16:03:54.02 Lysis1.in :DAmix
@16:03:54.41 <Lysis2mixer> Lysis2.seek 1480uL
@16:03:54.45 <PROCESSING> Lysis1.seek 575uL
@16:04:29.55 delay 5 seconds
@16:04:34.56 Handoff.select SPR
@16:04:34.68 SPR.command \"start 2 14\"
@16:04:36.15 SPR stabilized
@16:04:36.28 delay 10 seconds
@16:04:46.30 SPR.command \"start 1 0\"
@16:04:49.51 Waiting up to 4:00 for SPR to enter state 1
@16:06:52.34 SPR.command \"hold_on\"
@16:06:53.76 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@16:06:54.23 <Lysis2mixer> Lysis2.seek 1180uL
@16:06:54.27 <PROCESSING> Lysis1.seek 275uL
@16:07:36.28 delay 15 seconds
@16:07:51.30 SPR.command \"hold_off\"
@16:07:54.41 Waiting up to 38:00 for SPR to enter state 7
@16:28:44.24 <SPRlogger> SPRsummary:87.6707,83.1187,92.1307,88.5907 after 3527s
@16:35:21.47 <PROCESSING> SPR analysis completed
@16:35:29.63 <SPRlogger> Ended log spr22may2115h.spr.bz2
@16:35:29.64 <PROCESSING> SPR.command \"stats_off\"
@16:35:31.36 <LRAUV> -> Cmd.stop
@16:35:31.46 <closeHandoff> Handoff.select :close
@16:35:31.59 <LRAUV> Gate.power :heat,:OFF
@16:35:31.60 Gate.power :cartridge,:OFF
@16:35:31.69 Carousel.seek 47
@16:35:31.89 Plunge.seek :locked
@16:35:32.75 PinPort.in :unlocking
@16:35:32.81 PinPort.seek :clear
@16:35:33.35 <Archive> Archive.in :unlocking
@16:35:33.47 Archive.seek :clear
@16:35:33.51 <Air> Air.in :unlocking
@16:35:33.66 Air.seek :clear
@16:35:33.75 <Lysis1> Lysis1.in :unlocking
@16:35:33.92 Lysis1.seek :clear
@16:35:33.96 <Lysis2> Lysis2.in :unlocking
@16:35:34.07 Lysis2.seek :clear
@16:35:37.64 <LRAUV> Gate.power :motor,:OFF
@16:35:37.66 Gate.power :core,:OFF
@16:35:37.68 Gate.power :analytic1,:OFF
@16:35:37.70 Gate.power :analytic2,:OFF
@16:35:37.73 Gate.power :analytic3,:OFF
@16:35:37.75 Gate.power :main,:OFF
@16:35:37.77 Safely stopped and ready to power off
##-- file closed 2022-05-22T02:20:25.272Z