##-- file opened 2021-04-22T10:22:07.027Z
@03:22:07.11 -> showStatus
@03:22:09.13 -> Cmd.loadCartridge -6
@03:22:09.27 Gate.power :main,:ON,limit to 5000mA
@03:22:09.28 Gate.power :analytic1,:ON,limit to 5000mA
@03:22:09.31 Gate.power :analytic2,:ON,limit to 5000mA
@03:22:09.33 Gate.power :analytic3,:ON,limit to 5000mA
@03:22:09.47 Powering core bus
@03:22:11.73 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@03:22:11.87 <LRAUV> Archive.configure :default
@03:22:11.99 Air.configure :default
@03:22:12.09 Lysis1.configure :default
@03:22:12.20 Lysis2.configure :default
@03:22:12.32 Plunge.configure :default
@03:22:12.44 SamplePump.configure :default
@03:22:12.59 ExtraPump.configure :default
@03:22:12.69 Toroid.configure :default
@03:22:12.82 Carousel.configure :default
@03:22:12.94 Pressure.configure :default
@03:22:13.06 PinPort.configure :default
@03:22:13.12 All elf controllers running firmware version 4.741
@03:22:13.14 Gate.power :motor,:ON,limit to 5000mA
@03:22:13.32 <Archive_home> Archive.in :default
@03:22:13.66 <Toroid_home> Toroid.in :default
@03:22:13.74 <Plunge_home> Plunge.in :default
@03:22:13.77 <Lysis1_home> Lysis1.in :default
@03:22:13.80 <Archive_home> Archive.home.jog 0
@03:22:13.85 <PinPort_home> PinPort.in :default
@03:22:13.87 <Air_home> Air.in :default
@03:22:13.91 <Lysis2_home> Lysis2.in :default
@03:22:14.11 <Toroid_home> Toroid.home.jog 0
@03:22:14.16 <Lysis1_home> Lysis1.home.jog 0
@03:22:14.19 <Plunge_home> Plunge.home.jog 0
@03:22:14.22 <PinPort_home> PinPort.home.jog 0
@03:22:14.25 <Lysis2_home> Lysis2.home.jog 0
@03:22:14.29 <Air_home> Air.home.jog 0
@03:22:14.81 <Toroid_home> Toroid.in :homing
@03:22:14.85 <Plunge_home> Plunge.in :homing
@03:22:14.88 <Lysis1_home> Lysis1.in :homing
@03:22:14.92 <PinPort_home> PinPort.in :homing
@03:22:15.00 <Archive_home> Archive.in :homing
@03:22:15.53 <Plunge_home> Plunge.jog 2356
@03:22:15.58 <PinPort_home> PinPort.jog -2460
@03:22:15.60 <Toroid_home> Toroid.jog 626
@03:22:15.63 <Lysis1_home> Lysis1.jog 8672
@03:22:15.66 <Archive_home> Archive.jog 9333
@03:22:15.89 <Air_home> Air.in :homing
@03:22:15.91 <Lysis2_home> Lysis2.in :homing
@03:22:16.01 Lysis2.jog 4204
@03:22:16.04 <Air_home> Air.jog 9013
@03:22:17.23 <Plunge_home> Plunge.setRawPosition! 50
@03:22:17.27 Plunge.seek :home
@03:22:17.55 Plunge.in :default
@03:22:17.83 <Toroid_home> Toroid.setRawPosition! 0
@03:22:17.88 Toroid.seek :home
@03:22:17.96 Toroid.in :default
@03:22:18.34 <Lysis2_home> Lysis2.setRawPosition! 50
@03:22:18.38 Lysis2.seek :home
@03:22:18.66 Lysis2.in :default
@03:22:19.16 <PinPort_home> PinPort.setRawPosition! 0
@03:22:19.21 PinPort.seek :home
@03:22:19.29 PinPort.in :default
@03:22:19.87 <Lysis1_home> Lysis1.setRawPosition! 50
@03:22:19.92 Lysis1.seek :home
@03:22:20.16 <Archive_home> Archive.setRawPosition! 50
@03:22:20.21 Archive.seek :home
@03:22:20.28 <Lysis1_home> Lysis1.in :default
@03:22:20.36 <Air_home> Air.setRawPosition! 50
@03:22:20.41 Air.seek :home
@03:22:20.49 <Archive_home> Archive.in :default
@03:22:20.69 <Air_home> Air.in :default
@03:22:21.05 <LRAUV> Toroid.seek :clear
@03:22:22.67 Carousel.in :default
@03:22:22.73 Carousel.home.jog 0
@03:22:23.23 Carousel.in :default
@03:22:23.30 Carousel.seek 41
@03:22:23.46 Selecting Cartridge 42
@03:22:23.71 Carousel.seek 42
@03:22:24.19 Lysis1.in :fast
@03:22:24.25 Lysis1.seek :locked
@03:22:26.84 <Plunge> Plunge.seek :locked
@03:22:26.97 <Air> Air.in :fast
@03:22:27.03 Air.seek :locked
@03:22:29.62 <LRAUV> Powering cartridge bus
@03:22:31.74 Gate.setIR 1750,5000,0,6
@03:22:31.87 Gate.setIR 0,5000,0,6
@03:22:31.92 Gate.power :cartridge,:OFF
@03:22:32.46 Mounted archive Cartridge
@03:22:48.72 -> Cmd.startFiltering
@03:22:49.16 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@03:22:49.18 SamplePump.setPosition! 0ml
@03:22:49.22 Bypass.open
@03:22:50.68 Intake.open
@03:22:51.49 Sea 4.6m deep (21.5psia)
@03:22:51.51 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@03:22:54.08 Vacuum tare is 51% of full scale
@03:22:54.20 Pressure.in :default
@03:22:54.26 Pressure.hold
@03:22:54.43 SamplePump.in :prime
@03:22:54.50 SamplePump.seek 10ml,1:21
@03:23:03.88 Pressure.coast
@03:23:03.93 SamplePump.setPosition! 0ml
@03:23:03.98 Exhaust.open
@03:23:05.40 <closeBypass> Bypass.close
@03:23:06.95 <FILTERING> Pressure.in :default
@03:23:07.01 Pressure.hold
@03:23:07.12 Sea 5.5m deep (22.9psia)
@03:23:07.25 SamplePump.in :prime
@03:23:07.31 SamplePump.seek 100ml,8:51
@03:25:17.04 Pressure.coast
@03:25:17.10 Bypass.open
@03:25:17.80 Intake.close
@03:25:18.66 Toroid.in :hold
@03:25:18.72 Toroid.seek :crack
@03:25:20.27 Toroid.seek :cartridge
@03:25:21.36 Toroid.in :default
@03:25:21.42 Toroid.seek :clear
@03:25:23.38 Pressurizing cartridge to 26psi above 22.4psia
@03:25:23.39 SamplePump.setPosition! 0ml
@03:25:23.43 Intake.open
@03:25:24.17 Bypass.close
@03:25:24.86 Exhaust.close
@03:25:25.67 Pressure.in :default
@03:25:25.73 Pressure.hold
@03:25:25.90 SamplePump.in :charge
@03:25:25.96 SamplePump.seek 2.3ml,42 seconds
@03:25:32.05 Pressure.coast
@03:25:32.10 Intake.close
@03:25:32.91 Pressurized cartridge with 0.163ml to 26.5psi
@03:25:32.93 Isolated @43.1psia (20.7psi)
@03:25:32.94 Verifying cartridge holds at least 0.75..0.85 of 43.1psia
@03:25:32.98 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@03:25:37.60 Sampling 1000.0ml
@03:25:37.62 SamplePump.setPosition! 0ml
@03:25:37.67 Exhaust.open
@03:25:38.41 Intake.open
@03:25:39.27 Pressure.in :default
@03:25:39.33 Pressure.hold
@03:25:39.45 Sea 4.9m deep (22.0psia)
@03:25:39.58 SamplePump.in :sample
@03:25:39.65 SamplePump.seek 1000ml,1:23:53
@03:25:42.31 <sampling> Cartridge 42 filtering 1/1000ml at 0.33ml/s with 27.4psi vacuum 3.6m deep (20.1psia)
@03:26:42.31 Cartridge 42 filtering 19/1000ml at 0.30ml/s with 21.5psi vacuum 4.0m deep (20.7psia)
@03:27:42.31 Cartridge 42 filtering 37/1000ml at 0.29ml/s with 29.7psi vacuum
@03:28:42.31 Cartridge 42 filtering 55/1000ml at 0.33ml/s with 22.3psi vacuum 4.2m deep (20.8psia)
@03:29:42.31 Cartridge 42 filtering 73/1000ml at 0.25ml/s with 21.2psi vacuum 2.4m deep (18.3psia)
@03:30:42.31 Cartridge 42 filtering 90/1000ml at 0.32ml/s with 26.1psi vacuum 3.0m deep (19.1psia)
@03:31:42.31 Cartridge 42 filtering 107/1000ml at 0.27ml/s with 20.4psi vacuum 6.0m deep (23.6psia)
@03:32:42.31 Cartridge 42 filtering 123/1000ml at 0.27ml/s with 28.8psi vacuum 1.7m deep (17.3psia)
@03:33:42.31 Cartridge 42 filtering 140/1000ml at 0.31ml/s with 23.6psi vacuum 6.2m deep (23.9psia)
@03:34:42.31 Cartridge 42 filtering 156/1000ml at 0.29ml/s with 26.2psi vacuum 2.5m deep (18.5psia)
@03:35:42.31 Cartridge 42 filtering 172/1000ml at 0.25ml/s with 27.8psi vacuum 4.8m deep (21.7psia)
@03:36:42.31 Cartridge 42 filtering 187/1000ml at 0.27ml/s with 21.4psi vacuum 3.1m deep (19.2psia)
@03:37:42.31 Cartridge 42 filtering 203/1000ml at 0.23ml/s with 28.3psi vacuum 3.4m deep (19.7psia)
@03:38:42.31 Cartridge 42 filtering 218/1000ml at 0.26ml/s with 26.8psi vacuum 2.3m deep (18.1psia)
@03:39:42.32 Cartridge 42 filtering 233/1000ml at 0.21ml/s with 27.2psi vacuum 1.9m deep (17.6psia)
@03:40:42.31 Cartridge 42 filtering 248/1000ml at 0.26ml/s with 24.5psi vacuum 3.9m deep (20.5psia)
@03:41:42.31 Cartridge 42 filtering 262/1000ml at 0.25ml/s with 22.7psi vacuum 2.7m deep (18.7psia)
@03:42:42.31 Cartridge 42 filtering 277/1000ml at 0.25ml/s with 20.5psi vacuum 5.0m deep (22.0psia)
@03:43:42.31 Cartridge 42 filtering 291/1000ml at 0.22ml/s with 20.6psi vacuum 3.4m deep (19.7psia)
@03:44:42.31 Cartridge 42 filtering 305/1000ml at 0.24ml/s with 22.8psi vacuum 4.1m deep (20.8psia)
@03:45:42.31 Cartridge 42 filtering 319/1000ml at 0.21ml/s with 23.9psi vacuum 3.0m deep (19.1psia)
@03:46:42.31 Cartridge 42 filtering 332/1000ml at 0.22ml/s with 20.4psi vacuum 5.7m deep (23.1psia)
@03:47:42.31 Cartridge 42 filtering 346/1000ml at 0.20ml/s with 24.5psi vacuum 2.4m deep (18.3psia)
@03:48:42.31 Cartridge 42 filtering 359/1000ml at 0.22ml/s with 19.3psi vacuum 6.5m deep (24.2psia)
@03:49:42.31 Cartridge 42 filtering 373/1000ml at 0.20ml/s with 25.9psi vacuum 2.4m deep (18.2psia)
@03:50:42.31 Cartridge 42 filtering 386/1000ml at 0.22ml/s with 23.6psi vacuum 2.0m deep (17.7psia)
@03:51:42.31 Cartridge 42 filtering 399/1000ml at 0.24ml/s with 20.8psi vacuum 5.3m deep (22.5psia)
@03:52:42.31 Cartridge 42 filtering 412/1000ml at 0.21ml/s with 23.2psi vacuum 2.1m deep (17.8psia)
@03:53:42.31 Cartridge 42 filtering 425/1000ml at 0.24ml/s with 22.0psi vacuum 5.9m deep (23.4psia)
@03:54:42.31 Cartridge 42 filtering 438/1000ml at 0.23ml/s with 22.0psi vacuum 3.5m deep (19.9psia)
@03:55:42.31 Cartridge 42 filtering 450/1000ml at 0.21ml/s with 23.2psi vacuum 5.1m deep (22.2psia)
@03:56:42.31 Cartridge 42 filtering 463/1000ml at 0.20ml/s with 26.6psi vacuum 2.3m deep (18.2psia)
@03:57:42.31 Cartridge 42 filtering 476/1000ml at 0.21ml/s with 25.2psi vacuum 5.0m deep (22.1psia)
@03:58:42.31 Cartridge 42 filtering 488/1000ml at 0.20ml/s with 24.7psi vacuum
@03:59:42.31 Cartridge 42 filtering 500/1000ml at 0.20ml/s with 25.3psi vacuum 5.2m deep (22.3psia)
@04:00:42.31 Cartridge 42 filtering 513/1000ml at 0.20ml/s with 30.1psi vacuum 1.6m deep (17.1psia)
@04:01:42.31 Cartridge 42 filtering 525/1000ml at 0.20ml/s with 24.8psi vacuum 1.9m deep (17.5psia)
@04:02:42.31 Cartridge 42 filtering 537/1000ml at 0.20ml/s with 28.2psi vacuum 3.3m deep (19.6psia)
@04:03:42.31 Cartridge 42 filtering 550/1000ml at 0.20ml/s with 26.1psi vacuum 1.8m deep (17.3psia)
@04:03:45.20 <FILTERING> Flow averaged 0.24ml/s over 38:06
@04:03:45.23 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi
@04:03:45.80 Relieved excess vacuum in .6 seconds
@04:03:45.83 SamplePump.seek 1000ml,38:00
@04:04:02.56 Flow averaged 0.20ml/s over 17 seconds
@04:04:02.59 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi
@04:04:03.18 Relieved excess vacuum in .6 seconds
@04:04:03.20 SamplePump.seek 1000ml,37:43
@04:04:42.31 <sampling> Cartridge 42 filtering 562/1000ml at 0.20ml/s with 22.4psi vacuum 4.7m deep (21.7psia)
@04:05:08.98 <FILTERING> Flow averaged 0.20ml/s over 1:06
@04:05:09.01 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi
@04:05:09.58 Relieved excess vacuum in .6 seconds
@04:05:09.61 SamplePump.seek 1000ml,36:36
@04:05:42.31 <sampling> Cartridge 42 filtering 574/1000ml at 0.20ml/s with 27.4psi vacuum 4.6m deep (21.5psia)
@04:06:18.69 <FILTERING> Flow averaged 0.20ml/s over 1:10
@04:06:18.72 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi
@04:06:19.29 Relieved excess vacuum in .6 seconds
@04:06:19.32 SamplePump.seek 1000ml,35:26
@04:06:31.29 Flow averaged 0.20ml/s over 12 seconds
@04:06:31.31 <closeIntake> Intake.close
@04:06:31.38 <closeExhaust> Exhaust.close
@04:06:31.54 <FILTERING> Toroid.in :hold
@04:06:31.60 Toroid.seek :bypass
@04:06:33.23 Toroid.in :default
@04:06:33.29 Toroid.seek :clear
@04:06:34.91 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@04:06:34.92 Pressure.coast
@04:06:35.11 Sampled  583.4ml
@04:06:37.25 <LRAUV> -> Cmd.startProcessing
@04:06:37.62 <PROCESSING> Plunge.seek :reagent
@04:06:38.45 Sample loop closed. Ready to Deliver Reagents
@04:06:38.46 Archive.seek :sealed
@04:06:42.73 Archive.seek :evac
@04:06:56.66 delay 1:00
@04:07:56.69 Archive.seek :clear
@04:08:14.39 RNAlater soak for 10:00
@04:18:14.41 Archive.seek :tVent
@04:18:16.98 delay 1 second
@04:18:18.00 Archive.seek :evac
@04:18:33.62 delay 10 seconds
@04:18:43.65 Sample preserved and evacuated
@04:18:44.88 <LRAUV> -> Cmd.stop
@04:18:45.02 Carousel.seek 42
@04:18:45.19 Plunge.seek :locked
@04:18:46.14 <Archive> Archive.in :unlocking
@04:18:46.26 Archive.seek :clear
@04:18:46.30 <Air> Air.in :unlocking
@04:18:46.44 Air.seek :clear
@04:18:46.53 <Lysis1> Lysis1.in :unlocking
@04:18:46.65 Lysis1.seek :clear
@04:18:50.36 <LRAUV> Archive.coast
@04:18:50.41 Air.coast
@04:18:50.46 Lysis1.coast
@04:18:50.51 Lysis2.coast
@04:18:50.56 Plunge.coast
@04:18:50.61 SamplePump.coast
@04:18:50.66 Toroid.coast
@04:18:50.72 Carousel.coast
@04:18:50.77 PinPort.coast
@04:18:50.95 Gate.power :motor,:OFF
@04:18:50.97 Gate.power :core,:OFF
@04:18:50.99 Gate.power :analytic1,:OFF
@04:18:51.01 Gate.power :analytic2,:OFF
@04:18:51.03 Gate.power :analytic3,:OFF
@04:18:51.05 Gate.power :main,:OFF
@04:18:51.07 Safely stopped and ready to power off
##-- file closed 2021-04-22T11:30:46.254Z

##-- file opened 2021-04-22T11:30:46.256Z
@04:30:46.12 -> showStatus
@04:30:48.13 -> Cmd.loadCartridge -9
@04:30:48.27 Gate.power :main,:ON,limit to 5000mA
@04:30:48.29 Gate.power :analytic1,:ON,limit to 5000mA
@04:30:48.31 Gate.power :analytic2,:ON,limit to 5000mA
@04:30:48.34 Gate.power :analytic3,:ON,limit to 5000mA
@04:30:48.47 Powering core bus
@04:30:50.75 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@04:30:50.88 <LRAUV> Archive.configure :default
@04:30:51.00 Air.configure :default
@04:30:51.10 Lysis1.configure :default
@04:30:51.21 Lysis2.configure :default
@04:30:51.33 Plunge.configure :default
@04:30:51.46 SamplePump.configure :default
@04:30:51.61 ExtraPump.configure :default
@04:30:51.71 Toroid.configure :default
@04:30:51.84 Carousel.configure :default
@04:30:51.97 Pressure.configure :default
@04:30:52.08 PinPort.configure :default
@04:30:52.15 All elf controllers running firmware version 4.741
@04:30:52.18 Gate.power :motor,:ON,limit to 5000mA
@04:30:52.49 <Archive_home> Archive.in :default
@04:30:52.65 Archive.home.jog 0
@04:30:52.82 <PinPort_home> PinPort.in :default
@04:30:52.90 <Toroid_home> Toroid.in :default
@04:30:52.93 <Lysis2_home> Lysis2.in :default
@04:30:52.97 <Lysis1_home> Lysis1.in :default
@04:30:53.02 <Plunge_home> Plunge.in :default
@04:30:53.08 <Air_home> Air.in :default
@04:30:53.28 <PinPort_home> PinPort.home.jog 0
@04:30:53.32 <Toroid_home> Toroid.home.jog 0
@04:30:53.34 <Lysis2_home> Lysis2.home.jog 0
@04:30:53.37 <Lysis1_home> Lysis1.home.jog 0
@04:30:53.39 <Air_home> Air.home.jog 0
@04:30:53.43 <Plunge_home> Plunge.home.jog 0
@04:30:53.85 <Archive_home> Archive.in :homing
@04:30:54.44 <Toroid_home> Toroid.in :homing
@04:30:54.50 <Plunge_home> Plunge.in :homing
@04:30:54.54 <Air_home> Air.in :homing
@04:30:54.57 <PinPort_home> PinPort.in :homing
@04:30:54.60 <Lysis2_home> Lysis2.in :homing
@04:30:54.64 <Archive_home> Archive.jog 152
@04:30:54.69 <Lysis1_home> Lysis1.in :homing
@04:30:54.88 <Toroid_home> Toroid.jog 620
@04:30:54.91 <Plunge_home> Plunge.jog 1577
@04:30:54.94 <PinPort_home> PinPort.jog -107
@04:30:54.96 <Air_home> Air.jog 158
@04:30:54.99 <Lysis2_home> Lysis2.jog 150
@04:30:55.02 <Lysis1_home> Lysis1.jog 1418
@04:30:55.27 <Archive_home> Archive.setRawPosition! 50
@04:30:55.31 Archive.seek :home
@04:30:55.40 <PinPort_home> PinPort.setRawPosition! 0
@04:30:55.45 PinPort.seek :home
@04:30:55.55 PinPort.in :default
@04:30:55.60 <Air_home> Air.setRawPosition! 50
@04:30:55.67 Air.seek :home
@04:30:55.76 <Lysis2_home> Lysis2.setRawPosition! 50
@04:30:55.78 <Archive_home> Archive.in :default
@04:30:55.85 <Lysis2_home> Lysis2.seek :home
@04:30:55.96 <Air_home> Air.in :default
@04:30:56.16 <Lysis2_home> Lysis2.in :default
@04:30:56.29 <Plunge_home> Plunge.setRawPosition! 50
@04:30:56.34 Plunge.seek :home
@04:30:56.43 <Lysis1_home> Lysis1.setRawPosition! 50
@04:30:56.48 Lysis1.seek :home
@04:30:56.62 <Plunge_home> Plunge.in :default
@04:30:56.75 <Lysis1_home> Lysis1.in :default
@04:30:57.14 <Toroid_home> Toroid.setRawPosition! 0
@04:30:57.19 Toroid.seek :home
@04:30:57.27 Toroid.in :default
@04:30:57.40 <LRAUV> Toroid.seek :clear
@04:30:59.02 Carousel.in :default
@04:30:59.08 Carousel.home.jog 0
@04:30:59.58 Carousel.in :default
@04:30:59.64 Carousel.seek 42
@04:30:59.79 Selecting Cartridge 39
@04:31:00.04 Carousel.seek 39
@04:31:00.92 Lysis1.in :fast
@04:31:00.98 Lysis1.seek :locked
@04:31:03.58 <Plunge> Plunge.seek :locked
@04:31:03.71 <Air> Air.in :fast
@04:31:03.77 Air.seek :locked
@04:31:06.38 <LRAUV> Powering cartridge bus
@04:31:08.50 Gate.setIR 1750,5000,0,6
@04:31:08.64 Gate.setIR 0,5000,0,6
@04:31:08.70 PuckHeater.configure :default
@04:31:08.75 Gate.power :heat,:ON,limit to 5000mA
@04:31:09.43 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05084
@04:31:09.47 Using default puck heat loss curve for HeatingAccelInCarousel
@04:31:28.54 -> Cmd.startFiltering
@04:31:28.98 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@04:31:29.00 SamplePump.setPosition! 0ml
@04:31:29.04 Bypass.open
@04:31:30.51 Intake.open
@04:31:31.32 Sea 4.8m deep (21.8psia)
@04:31:31.34 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@04:31:33.92 Vacuum tare is 51% of full scale
@04:31:34.04 Pressure.in :default
@04:31:34.10 Pressure.hold
@04:31:34.27 SamplePump.in :prime
@04:31:34.34 SamplePump.seek 10ml,1:21
@04:31:43.72 Pressure.coast
@04:31:43.77 SamplePump.setPosition! 0ml
@04:31:43.82 Exhaust.open
@04:31:45.23 <closeBypass> Bypass.close
@04:31:46.81 <FILTERING> Pressure.in :default
@04:31:46.87 Pressure.hold
@04:31:46.97 Sea 5.5m deep (22.8psia)
@04:31:47.10 SamplePump.in :prime
@04:31:47.17 SamplePump.seek 100ml,8:51
@04:33:56.80 Pressure.coast
@04:33:56.86 Bypass.open
@04:33:57.59 Intake.close
@04:33:58.44 Toroid.in :hold
@04:33:58.50 Toroid.seek :crack
@04:34:00.06 Toroid.seek :cartridge
@04:34:01.16 Toroid.in :default
@04:34:01.22 Toroid.seek :clear
@04:34:03.19 Pressurizing cartridge to 26psi above 23.9psia
@04:34:03.20 SamplePump.setPosition! 0ml
@04:34:03.24 Intake.open
@04:34:03.95 Bypass.close
@04:34:04.67 Exhaust.close
@04:34:05.46 Pressure.in :default
@04:34:05.52 Pressure.hold
@04:34:05.68 SamplePump.in :charge
@04:34:05.75 SamplePump.seek 2.3ml,42 seconds
@04:34:14.22 Pressure.coast
@04:34:14.27 Intake.close
@04:34:15.09 Pressurized cartridge with 0.318ml to 26.1psi
@04:34:15.10 Isolated @45.3psia (21.4psi)
@04:34:15.12 Verifying cartridge holds at least 0.75..0.85 of 45.3psia
@04:34:15.15 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@04:34:19.77 Sampling 1000.0ml
@04:34:19.79 SamplePump.setPosition! 0ml
@04:34:19.84 Exhaust.open
@04:34:20.55 Intake.open
@04:34:21.42 Pressure.in :default
@04:34:21.49 Pressure.hold
@04:34:21.60 Sea 6.4m deep (24.2psia)
@04:34:21.73 SamplePump.in :sample
@04:34:21.80 SamplePump.seek 1000ml,1:23:53
@04:34:24.46 <sampling> Cartridge 39 filtering 1/1000ml at 0.32ml/s with 20.2psi vacuum 3.8m deep (20.3psia)
@04:35:24.45 Cartridge 39 filtering 20/1000ml at 0.31ml/s with 20.7psi vacuum 2.6m deep (18.5psia)
@04:36:24.46 Cartridge 39 filtering 38/1000ml at 0.33ml/s with 25.2psi vacuum 3.5m deep (19.8psia)
@04:37:24.51 Cartridge 39 filtering 56/1000ml at 0.26ml/s with 26.4psi vacuum
@04:38:24.46 Cartridge 39 filtering 73/1000ml at 0.25ml/s with 24.6psi vacuum 3.5m deep (19.9psia)
@04:39:24.45 Cartridge 39 filtering 90/1000ml at 0.30ml/s with 26.2psi vacuum 3.9m deep (20.4psia)
@04:40:24.46 Cartridge 39 filtering 107/1000ml at 0.27ml/s with 23.3psi vacuum 3.5m deep (19.8psia)
@04:41:24.45 Cartridge 39 filtering 123/1000ml at 0.28ml/s with 27.6psi vacuum 3.5m deep (19.8psia)
@04:42:24.46 Cartridge 39 filtering 139/1000ml at 0.24ml/s with 21.9psi vacuum 3.1m deep (19.3psia)
@04:43:24.45 Cartridge 39 filtering 154/1000ml at 0.26ml/s with 23.1psi vacuum 3.7m deep (20.1psia)
@04:44:24.46 Cartridge 39 filtering 169/1000ml at 0.28ml/s with 22.6psi vacuum 2.8m deep (18.8psia)
@04:45:24.45 Cartridge 39 filtering 184/1000ml at 0.27ml/s with 25.5psi vacuum 4.5m deep (21.4psia)
@04:46:24.46 Cartridge 39 filtering 199/1000ml at 0.21ml/s with 22.1psi vacuum 1.9m deep (17.5psia)
@04:47:24.45 Cartridge 39 filtering 214/1000ml at 0.27ml/s with 22.9psi vacuum 4.9m deep (21.9psia)
@04:48:24.46 Cartridge 39 filtering 228/1000ml at 0.23ml/s with 22.5psi vacuum 3.4m deep (19.7psia)
@04:49:24.45 Cartridge 39 filtering 242/1000ml at 0.20ml/s with 22.3psi vacuum 3.0m deep (19.1psia)
@04:50:24.46 Cartridge 39 filtering 255/1000ml at 0.23ml/s with 22.7psi vacuum 4.5m deep (21.3psia)
@04:51:24.45 Cartridge 39 filtering 269/1000ml at 0.21ml/s with 28.3psi vacuum 1.6m deep (17.1psia)
@04:52:24.46 Cartridge 39 filtering 282/1000ml at 0.20ml/s with 26.1psi vacuum 3.7m deep (20.2psia)
@04:53:24.45 Cartridge 39 filtering 296/1000ml at 0.22ml/s with 23.1psi vacuum 3.3m deep (19.6psia)
@04:54:24.46 Cartridge 39 filtering 309/1000ml at 0.22ml/s with 24.0psi vacuum 3.6m deep (20.1psia)
@04:55:24.45 Cartridge 39 filtering 322/1000ml at 0.20ml/s with 25.6psi vacuum 2.2m deep (17.9psia)
@04:56:24.46 Cartridge 39 filtering 334/1000ml at 0.20ml/s with 26.5psi vacuum 4.4m deep (21.2psia)
@04:57:24.45 Cartridge 39 filtering 347/1000ml at 0.23ml/s with 23.0psi vacuum 2.8m deep (18.8psia)
@04:58:24.46 Cartridge 39 filtering 359/1000ml at 0.20ml/s with 25.1psi vacuum 4.1m deep (20.8psia)
@04:59:24.45 Cartridge 39 filtering 372/1000ml at 0.21ml/s with 21.8psi vacuum 4.5m deep (21.4psia)
@05:00:24.46 Cartridge 39 filtering 384/1000ml at 0.20ml/s with 27.4psi vacuum 2.3m deep (18.0psia)
@05:01:24.45 Cartridge 39 filtering 396/1000ml at 0.20ml/s with 26.0psi vacuum 3.7m deep (20.2psia)
@05:02:24.46 Cartridge 39 filtering 409/1000ml at 0.20ml/s with 25.8psi vacuum 2.4m deep (18.3psia)
@05:02:25.73 <FILTERING> Flow averaged 0.24ml/s over 28:04
@05:02:25.76 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi
@05:02:26.32 Relieved excess vacuum in .6 seconds
@05:02:26.35 SamplePump.seek 1000ml,49:48
@05:02:53.50 Flow averaged 0.20ml/s over 28 seconds
@05:02:53.53 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi
@05:02:54.09 Relieved excess vacuum in .6 seconds
@05:02:54.12 SamplePump.seek 1000ml,49:20
@05:03:06.28 Flow averaged 0.20ml/s over 13 seconds
@05:03:06.30 <closeIntake> Intake.close
@05:03:06.34 <closeExhaust> Exhaust.close
@05:03:06.53 <FILTERING> Toroid.in :hold
@05:03:06.59 Toroid.seek :bypass
@05:03:08.22 Toroid.in :default
@05:03:08.28 Toroid.seek :clear
@05:03:09.89 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@05:03:09.90 Pressure.coast
@05:03:10.08 Sampled  416.8ml
@05:03:12.25 <LRAUV> -> Cmd.startProcessing
@05:03:13.68 <PROCESSING> SPR.command \"read_sequences\"
@05:03:14.33 SPR.command \"stats_on\"
@05:03:14.37 SPR.command \"start 0 1\"
@05:03:16.38 <SPRlogger> Begun log /var/log/esp/spr21apr2205h.spr
@05:03:17.18 <PROCESSING> Handoff.select :waste
@05:03:23.86 Syringe tare is 20% of full scale
@05:03:23.88 PinPort.in :locking
@05:03:23.95 PinPort.seek :locked
@05:03:24.43 <posArchive> Archive.in :fast
@05:03:24.48 <posLyS2> Lysis2.in :fast
@05:03:24.52 <PROCESSING> Lysis1.seek 1350uL
@05:03:24.64 <posLyS2> Lysis2.seek :full
@05:03:24.68 <posArchive> Archive.seek :full
@05:03:26.74 <PROCESSING> Plunge.seek :reagent
@05:03:27.64 Evacuating puck with filter bubble point of 50psi
@05:03:27.66 PinPort.seek :locked
@05:03:27.74 Handoff.select :waste
@05:03:34.82 Air syringe pumping up to 1 stroke to reach 8psi
@05:03:34.83 Air stroke #1 of 1
@05:03:34.91 Air.in :pressurize
@05:03:34.97 Air.seek :pressurized
@05:03:46.22 0.0psi after pressurize
@05:03:46.23 Air.in :compress
@05:03:46.30 Air.seek :evac
@05:03:50.39 0.6psi after compress
@05:04:02.97 0.1psi after compress (stable)
@05:04:02.98 Air.in :default
@05:04:03.07 Air.hold
@05:04:03.25 at 0.0psi Opened to waste
@05:04:03.26 Handoff.select :blocked
@05:04:03.39 Archive syringe pumping up to 5 strokes to reach 50psi
@05:04:03.40 Archive stroke #1 of 5
@05:04:03.47 Archive.in :pressurize
@05:04:03.54 Archive.seek :pressurized
@05:04:16.30 11.5psi after pressurize
@05:04:16.32 Archive.in :compress
@05:04:16.40 Archive.seek :empty
@05:04:32.28 33.2psi after compress
@05:04:46.35 37.8psi after compress (stable)
@05:04:46.37 Archive.in :gulp
@05:04:46.44 Archive.seek :topVent
@05:04:53.64 37.9psi after gulp
@05:05:06.21 37.9psi after gulp (stable)
@05:05:06.22 Archive stroke #2 of 5
@05:05:06.31 Archive.in :pressurize
@05:05:06.37 Archive.seek :pressurized
@05:05:19.14 37.6psi after pressurize
@05:05:19.15 Archive.in :compress
@05:05:19.22 Archive.seek :empty
@05:05:31.97 Reached 50.2 of 50.0psi after pumping 3393uL (1.96 strokes)
@05:05:31.99 Archive.in :default
@05:05:32.06 Archive.hold
@05:05:32.13 Handoff.select :waste
@05:05:42.35 delay 1:00
@05:06:42.39 Handoff.select :blocked
@05:07:05.08 Handoff.select :waste
@05:07:05.20 0.4psi at evacEnd
@05:07:05.21 Handoff.select :blocked
@05:07:05.34 Archive.in :fast
@05:07:05.41 Archive.seek :topVent
@05:07:18.89 Stable @0.8psi after Handoff blocked
@05:07:18.90 Archive syringe pumping up to 5 strokes to reach 31psi
@05:07:18.92 Archive stroke #1 of 5
@05:07:19.02 Archive.in :pressurize
@05:07:19.08 Archive.seek :pressurized
@05:07:31.82 1.1psi after pressurize
@05:07:31.83 Archive.in :compress
@05:07:31.91 Archive.seek :empty
@05:07:47.81 2.8psi after compress
@05:08:38.39 27.4psi after compress (stable)
@05:08:38.41 Archive.in :gulp
@05:08:38.48 Archive.seek :topVent
@05:08:45.69 29.0psi after gulp
@05:08:58.27 29.8psi after gulp (stable)
@05:08:58.28 Archive stroke #2 of 5
@05:08:58.37 Archive.in :pressurize
@05:08:58.43 Archive.seek :pressurized
@05:09:11.17 30.8psi after pressurize
@05:09:11.18 Archive.in :compress
@05:09:11.25 Archive.seek :empty
@05:09:11.44 Reached 30.8 of 30.8psi after pumping 3124uL (1.81 strokes)
@05:09:11.46 Archive.in :default
@05:09:11.53 Archive.hold
@05:09:11.63 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position
@05:09:11.65 Handoff.select :waste
@05:09:25.32 delay 1:00
@05:10:25.34 Handoff.select :blocked
@05:10:55.94 Handoff.select :waste
@05:10:56.06 4.5psi at evacEnd
@05:10:56.08 Air.seek :fullVent
@05:10:57.52 Archive.in :fast
@05:10:57.58 Archive.seek :topVent
@05:11:03.55 Seawater evacuated, Presure above/below filter released. Lysis begins
@05:11:03.64 Lysis1.in :slow
@05:11:03.70 Lysis1.seek 1050uL
@05:11:33.74 delay 5 seconds
@05:11:38.76 Handoff.select :blocked
@05:11:38.93 Lysis1.seek 450uL
@05:12:38.92 delay 3 seconds
@05:12:41.99 Air.in to mix into 1.37806 parts Lysis1
@05:12:42.52 <Airmixer> Air.seek :evac
@05:12:42.56 <PROCESSING> Lysis1.seek 300uL
@05:12:57.68 Lysis1.seek :empty
@05:13:27.79 Lysis buffer delivered @20.5psi
@05:13:27.81 Polling Can every 2:00
@05:13:28.36 PuckHeater.configure :default #ambient 11.7C
@05:13:28.41 PuckHeater.seek 80.2C
@05:14:43.11 PuckHeater.hold 2:00,80.2C
@05:16:42.96 Polling Can every 2:00
@05:16:43.51 PuckHeater.configure :default #ambient 11.8C
@05:16:43.58 PuckHeater.hold 30 seconds,79.2C
@05:17:13.70 Polling Can every 2:00
@05:17:14.25 PuckHeater.configure :default #ambient 11.8C
@05:17:14.29 PuckHeater.hold 30 seconds,78.6C
@05:17:44.41 Polling Can every 2:00
@05:17:44.96 PuckHeater.hold 10:00,78.1C
@05:27:43.74 Lysis complete. Syringe pressure @17.4psi
@05:27:43.75 Handoff.select :blocked
@05:27:43.80 Archive syringe pumping up to 3 strokes to reach 40psi
Archive stroke #1 of 3
@05:27:43.89 Archive.in :pressurize
@05:27:43.96 Archive.seek :pressurized
@05:27:56.70 17.1psi after pressurize
@05:27:56.72 Archive.in :compress
@05:27:56.79 Archive.seek :empty
@05:28:05.81 Reached 40.7 of 40.0psi after pumping 1583uL (0.92 strokes)
@05:28:05.82 Archive.in :default
@05:28:05.88 Archive.hold
@05:28:05.95 delay 2 seconds
@05:28:07.97 Archive.in :fast
@05:28:08.03 Archive.seek :full
@05:28:14.73 Lysis1.seek 138uL
@05:28:29.00 delay 1 second
@05:28:30.03 <fillArchive> Archive.seek :full
@05:28:30.07 <PROCESSING> Lysis1.seek :empty
@05:28:44.05 Initial air pressure @43.3psi
@05:28:44.11 Lysis1.in :extract
@05:28:44.19 Lysis1.seek 600uL,nil
@05:28:46.41 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@05:28:46.42 Archive stroke #1 of 6
@05:28:46.55 Archive.in :pressurize
@05:28:46.62 Archive.seek :pressurized
@05:28:59.38 15.5psi after pressurize
@05:28:59.40 Archive.in :compress
@05:28:59.47 Archive.seek :empty
@05:29:14.87 Archive.in :default
@05:29:14.92 Archive.hold
@05:29:15.15 <PROCESSING> Initial 600uL lysate @19.6psi
@05:29:15.16 Handoff.select :blocked
@05:29:15.25 Initial air pressure @21.4psi
@05:29:15.33 Lysis1.seek 825uL,nil
@05:29:16.51 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@05:29:16.52 Archive stroke #1 of 6
@05:29:16.61 Archive.in :compress
@05:29:16.68 Archive.seek :empty
@05:29:17.19 15.0psi after compress
@05:29:17.59 Reached 17.2 of 17.0psi after pumping 1730uL (1.00 strokes)
@05:29:17.60 Archive.in :default
@05:29:17.67 Archive.hold
@05:29:17.83 Slide::Error in pump -- Slide::Error while holding Archive Syringe (ArS) in position
@05:29:18.47 Archive syringe pumping up to 6 strokes to reach 17psi
@05:29:18.48 Archive stroke #1 of 6
@05:29:18.56 Archive.in :compress
@05:29:18.63 Archive.seek :empty
@05:29:18.81 15.7psi after compress
@05:29:24.07 Reached 17.1 of 17.0psi after pumping 1730uL (1.00 strokes)
@05:29:24.08 Archive.in :default
@05:29:24.15 Archive.hold
@05:29:24.63 <PROCESSING> Recovered 825uL lysate @15.4psi
@05:29:24.65 Air.in :halfSpeed
@05:29:24.72 Air.seek :fullVent
@05:29:26.96 Puck Top Vented, bottom @2.3psi
@05:29:26.97 Handoff.select :waste
@05:29:27.09 delay 5 seconds
@05:29:32.15 Handoff open to waste, bottom @-0.0psi
@05:29:32.17 Air.in :default
@05:29:32.23 Air.seek :evac
@05:29:33.67 Lysis2.in :DAmix
@05:29:33.77 Lysis1.in :DAmix
@05:29:34.14 <Lysis2mixer> Lysis2.seek 1480uL
@05:29:34.19 <PROCESSING> Lysis1.seek 575uL
@05:30:09.26 delay 5 seconds
@05:30:14.28 Handoff.select SPR
@05:30:14.39 SPR.command \"start 2 14\"
@05:30:15.59 delay 10 seconds
@05:30:25.61 SPR.command \"start 1 0\"
@05:30:28.85 Waiting up to 4:00 for SPR to enter state 1
@05:32:31.23 SPR.command \"hold_on\"
@05:32:32.64 SPR Dilution 1 of 1 -- Running Experiment 1
@05:32:33.08 <Lysis2mixer> Lysis2.seek 1180uL
@05:32:33.16 <PROCESSING> Lysis1.seek 275uL
@05:33:15.17 volL1 = 300.0 and volL2 = 300.0
@05:33:15.18 delay 15 seconds
@05:33:30.20 SPR.command \"hold_off\"
@05:33:33.29 Waiting up to 38:00 for SPR to enter state 7
@06:00:16.84 SPR Analysis has Completed
@06:00:16.86 Handoff.select :waste
@06:00:17.77 <SPRlogger> Ended log /var/log/esp/spr21apr2205h.spr
@06:00:17.78 <PROCESSING> SPR.command \"stats_off\"
@06:00:19.53 <LRAUV> -> Cmd.stop
@06:00:19.62 <closeHandoff> Handoff.select :close
@06:00:19.74 <LRAUV> Gate.power :heat,:OFF
@06:00:19.76 Gate.power :cartridge,:OFF
@06:00:19.84 Carousel.seek 39
@06:00:20.01 Plunge.seek :locked
@06:00:20.92 PinPort.in :unlocking
@06:00:20.98 PinPort.seek :clear
@06:00:21.51 <Archive> Archive.in :unlocking
@06:00:21.64 Archive.seek :clear
@06:00:21.67 <Air> Air.in :unlocking
@06:00:21.83 Air.seek :clear
@06:00:21.91 <Lysis1> Lysis1.in :unlocking
@06:00:22.02 <Lysis2> Lysis2.in :unlocking
@06:00:22.06 <Lysis1> Lysis1.seek :clear
@06:00:22.16 <Lysis2> Lysis2.seek :clear
@06:00:25.72 <LRAUV> Archive.coast
@06:00:25.77 Air.coast
@06:00:25.82 Lysis1.coast
@06:00:25.87 Lysis2.coast
@06:00:25.93 Plunge.coast
@06:00:25.98 SamplePump.coast
@06:00:26.04 Toroid.coast
@06:00:26.08 Carousel.coast
@06:00:26.14 PinPort.coast
@06:00:26.31 Gate.power :motor,:OFF
@06:00:26.33 Gate.power :core,:OFF
@06:00:26.35 Gate.power :analytic1,:OFF
@06:00:26.37 Gate.power :analytic2,:OFF
@06:00:26.39 Gate.power :analytic3,:OFF
@06:00:26.41 Gate.power :main,:OFF
@06:00:26.43 Safely stopped and ready to power off
##-- file closed 2021-04-22T15:07:54.690Z

##-- file opened 2021-04-22T15:07:54.693Z
@08:07:55.09 -> showStatus
@08:07:57.12 -> Cmd.loadCartridge -6
@08:07:57.27 Gate.power :main,:ON,limit to 5000mA
@08:07:57.28 Gate.power :analytic1,:ON,limit to 5000mA
@08:07:57.31 Gate.power :analytic2,:ON,limit to 5000mA
@08:07:57.33 Gate.power :analytic3,:ON,limit to 5000mA
@08:07:57.46 Powering core bus
@08:07:59.74 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@08:07:59.87 <LRAUV> Archive.configure :default
@08:08:00.00 Air.configure :default
@08:08:00.10 Lysis1.configure :default
@08:08:00.21 Lysis2.configure :default
@08:08:00.33 Plunge.configure :default
@08:08:00.45 SamplePump.configure :default
@08:08:00.60 ExtraPump.configure :default
@08:08:00.72 Toroid.configure :default
@08:08:00.84 Carousel.configure :default
@08:08:00.96 Pressure.configure :default
@08:08:01.08 PinPort.configure :default
@08:08:01.14 All elf controllers running firmware version 4.741
@08:08:01.17 Gate.power :motor,:ON,limit to 5000mA
@08:08:01.27 <Archive_home> Archive.in :default
@08:08:01.53 Archive.home.jog 0
@08:08:01.78 <PinPort_home> PinPort.in :default
@08:08:01.86 <Toroid_home> Toroid.in :default
@08:08:01.89 <Plunge_home> Plunge.in :default
@08:08:01.94 <Lysis1_home> Lysis1.in :default
@08:08:01.99 <Lysis2_home> Lysis2.in :default
@08:08:02.04 <Air_home> Air.in :default
@08:08:02.23 <Toroid_home> Toroid.home.jog 0
@08:08:02.28 <PinPort_home> PinPort.home.jog 0
@08:08:02.31 <Plunge_home> Plunge.home.jog 0
@08:08:02.33 <Lysis1_home> Lysis1.home.jog 0
@08:08:02.37 <Air_home> Air.home.jog 0
@08:08:02.41 <Lysis2_home> Lysis2.home.jog 0
@08:08:02.81 <Archive_home> Archive.in :homing
@08:08:03.31 Archive.jog 150
@08:08:03.48 <Toroid_home> Toroid.in :homing
@08:08:03.51 <Plunge_home> Plunge.in :homing
@08:08:03.55 <Air_home> Air.in :homing
@08:08:03.59 <Lysis1_home> Lysis1.in :homing
@08:08:03.64 <PinPort_home> PinPort.in :homing
@08:08:03.68 <Lysis2_home> Lysis2.in :homing
@08:08:03.81 <Toroid_home> Toroid.jog 626
@08:08:03.90 <Plunge_home> Plunge.jog 1575
@08:08:03.92 <Air_home> Air.jog 170
@08:08:03.95 <PinPort_home> PinPort.jog -107
@08:08:03.97 <Lysis1_home> Lysis1.jog 1416
@08:08:04.03 <Lysis2_home> Lysis2.jog 151
@08:08:04.28 <Archive_home> Archive.setRawPosition! 50
@08:08:04.33 Archive.seek :home
@08:08:04.41 <PinPort_home> PinPort.setRawPosition! 0
@08:08:04.46 PinPort.seek :home
@08:08:04.55 PinPort.in :default
@08:08:04.65 <Lysis2_home> Lysis2.setRawPosition! 50
@08:08:04.68 <Archive_home> Archive.in :default
@08:08:04.73 <Air_home> Air.setRawPosition! 50
@08:08:04.79 <Lysis2_home> Lysis2.seek :home
@08:08:04.88 <Air_home> Air.seek :home
@08:08:05.13 <Lysis2_home> Lysis2.in :default
@08:08:05.22 <Air_home> Air.in :default
@08:08:05.33 <Lysis1_home> Lysis1.setRawPosition! 50
@08:08:05.35 <Plunge_home> Plunge.setRawPosition! 50
@08:08:05.41 <Lysis1_home> Lysis1.seek :home
@08:08:05.46 <Plunge_home> Plunge.seek :home
@08:08:05.74 <Lysis1_home> Lysis1.in :default
@08:08:05.82 <Plunge_home> Plunge.in :default
@08:08:06.13 <Toroid_home> Toroid.setRawPosition! 0
@08:08:06.18 Toroid.seek :home
@08:08:06.27 Toroid.in :default
@08:08:06.40 <LRAUV> Toroid.seek :clear
@08:08:08.01 Carousel.in :default
@08:08:08.07 Carousel.home.jog 0
@08:08:08.56 Carousel.in :default
@08:08:08.62 Carousel.seek 39
@08:08:08.78 Selecting Cartridge 40
@08:08:09.01 Carousel.seek 40
@08:08:09.50 Lysis1.in :fast
@08:08:09.56 Lysis1.seek :locked
@08:08:12.15 <Plunge> Plunge.seek :locked
@08:08:12.29 <Air> Air.in :fast
@08:08:12.35 Air.seek :locked
@08:08:14.95 <LRAUV> Powering cartridge bus
@08:08:17.07 Gate.setIR 1750,5000,0,6
@08:08:17.21 Gate.setIR 0,5000,0,6
@08:08:17.26 Gate.power :cartridge,:OFF
@08:08:17.80 Mounted archive Cartridge
@08:08:36.71 -> Cmd.startFiltering
@08:08:37.17 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@08:08:37.18 SamplePump.setPosition! 0ml
@08:08:37.23 Bypass.open
@08:08:38.68 Intake.open
@08:08:39.45 Sea 6.4m deep (24.2psia)
@08:08:39.47 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@08:08:42.04 Vacuum tare is 51% of full scale
@08:08:42.17 Pressure.in :default
@08:08:42.23 Pressure.hold
@08:08:42.40 SamplePump.in :prime
@08:08:42.47 SamplePump.seek 10ml,1:21
@08:08:51.86 Pressure.coast
@08:08:51.91 SamplePump.setPosition! 0ml
@08:08:51.96 Exhaust.open
@08:08:53.38 <closeBypass> Bypass.close
@08:08:54.90 <FILTERING> Pressure.in :default
@08:08:54.96 Pressure.hold
@08:08:55.07 Sea 6.9m deep (24.9psia)
@08:08:55.20 SamplePump.in :prime
@08:08:55.26 SamplePump.seek 100ml,8:51
@08:11:04.76 Pressure.coast
@08:11:04.82 Bypass.open
@08:11:05.53 Intake.close
@08:11:06.37 Toroid.in :hold
@08:11:06.43 Toroid.seek :crack
@08:11:07.98 Toroid.seek :cartridge
@08:11:09.09 Toroid.in :default
@08:11:09.15 Toroid.seek :clear
@08:11:11.11 Pressurizing cartridge to 26psi above 24.7psia
@08:11:11.12 SamplePump.setPosition! 0ml
@08:11:11.16 Intake.open
@08:11:11.92 Bypass.close
@08:11:12.67 Exhaust.close
@08:11:13.50 Pressure.in :default
@08:11:13.56 Pressure.hold
@08:11:13.73 SamplePump.in :charge
@08:11:13.79 SamplePump.seek 2.3ml,42 seconds
@08:11:19.39 Pressure.coast
@08:11:19.44 Intake.close
@08:11:20.27 Pressurized cartridge with 0.138ml to 26.0psi
@08:11:20.28 Isolated @45.2psia (20.4psi)
@08:11:20.29 Verifying cartridge holds at least 0.75..0.85 of 45.2psia
@08:11:20.33 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@08:11:24.95 Sampling 1000.0ml
@08:11:24.97 SamplePump.setPosition! 0ml
@08:11:25.02 Exhaust.open
@08:11:25.75 Intake.open
@08:11:26.60 Pressure.in :default
@08:11:26.66 Pressure.hold
@08:11:26.78 Sea 6.6m deep (24.4psia)
@08:11:26.91 SamplePump.in :sample
@08:11:26.98 SamplePump.seek 1000ml,1:23:53
@08:11:29.64 <sampling> Cartridge 40 filtering 1/1000ml at 0.32ml/s with 29.9psi vacuum 2.4m deep (18.2psia)
@08:12:29.64 Cartridge 40 filtering 19/1000ml at 0.35ml/s with 22.2psi vacuum 3.9m deep (20.5psia)
@08:13:29.64 Cartridge 40 filtering 38/1000ml at 0.34ml/s with 22.9psi vacuum 5.2m deep (22.4psia)
@08:14:29.64 Cartridge 40 filtering 56/1000ml at 0.30ml/s with 23.3psi vacuum
@08:15:29.64 Cartridge 40 filtering 73/1000ml at 0.33ml/s with 23.0psi vacuum 5.7m deep (23.0psia)
@08:16:29.64 Cartridge 40 filtering 90/1000ml at 0.29ml/s with 27.4psi vacuum 3.4m deep (19.8psia)
@08:17:29.64 Cartridge 40 filtering 107/1000ml at 0.29ml/s with 25.4psi vacuum 4.2m deep (20.9psia)
@08:18:29.64 Cartridge 40 filtering 123/1000ml at 0.23ml/s with 25.6psi vacuum 3.4m deep (19.8psia)
@08:19:29.64 Cartridge 40 filtering 139/1000ml at 0.26ml/s with 20.0psi vacuum 3.6m deep (20.0psia)
@08:20:29.64 Cartridge 40 filtering 155/1000ml at 0.28ml/s with 22.3psi vacuum 5.8m deep (23.3psia)
@08:21:30.05 Cartridge 40 filtering 171/1000ml at 0.29ml/s with 22.2psi vacuum 3.9m deep (20.5psia)
@08:22:29.64 Cartridge 40 filtering 186/1000ml at 0.24ml/s with 28.1psi vacuum 4.4m deep (21.2psia)
@08:23:29.64 Cartridge 40 filtering 201/1000ml at 0.27ml/s with 23.8psi vacuum 4.0m deep (20.6psia)
@08:24:29.64 Cartridge 40 filtering 216/1000ml at 0.21ml/s with 22.5psi vacuum 2.9m deep (19.0psia)
@08:25:29.64 Cartridge 40 filtering 231/1000ml at 0.21ml/s with 23.0psi vacuum 4.7m deep (21.6psia)
@08:26:29.64 Cartridge 40 filtering 245/1000ml at 0.24ml/s with 20.9psi vacuum 3.5m deep (19.9psia)
@08:27:29.64 Cartridge 40 filtering 259/1000ml at 0.24ml/s with 25.5psi vacuum 5.6m deep (23.0psia)
@08:28:29.64 Cartridge 40 filtering 273/1000ml at 0.25ml/s with 26.5psi vacuum 2.8m deep (18.8psia)
@08:29:29.64 Cartridge 40 filtering 287/1000ml at 0.20ml/s with 24.9psi vacuum 3.2m deep (19.5psia)
@08:30:29.64 Cartridge 40 filtering 300/1000ml at 0.25ml/s with 25.4psi vacuum 4.0m deep (20.6psia)
@08:31:29.64 Cartridge 40 filtering 314/1000ml at 0.23ml/s with 27.7psi vacuum 2.5m deep (18.4psia)
@08:32:29.64 Cartridge 40 filtering 327/1000ml at 0.22ml/s with 22.7psi vacuum 5.2m deep (22.4psia)
@08:33:29.64 Cartridge 40 filtering 340/1000ml at 0.22ml/s with 24.5psi vacuum 2.3m deep (18.1psia)
@08:34:29.64 Cartridge 40 filtering 353/1000ml at 0.20ml/s with 25.3psi vacuum 5.5m deep (22.8psia)
@08:35:29.64 Cartridge 40 filtering 366/1000ml at 0.20ml/s with 23.4psi vacuum 1.7m deep (17.3psia)
@08:36:29.64 Cartridge 40 filtering 378/1000ml at 0.20ml/s with 25.1psi vacuum 4.0m deep (20.6psia)
@08:37:29.64 Cartridge 40 filtering 391/1000ml at 0.20ml/s with 27.0psi vacuum 2.9m deep (19.0psia)
@08:38:29.64 Cartridge 40 filtering 403/1000ml at 0.20ml/s with 26.7psi vacuum 1.7m deep (17.3psia)
@08:39:29.64 Cartridge 40 filtering 416/1000ml at 0.20ml/s with 27.6psi vacuum 3.6m deep (20.1psia)
@08:40:29.64 Cartridge 40 filtering 428/1000ml at 0.20ml/s with 25.4psi vacuum 1.8m deep (17.3psia)
@08:41:29.64 Cartridge 40 filtering 440/1000ml at 0.21ml/s with 21.7psi vacuum 5.9m deep (23.4psia)
@08:42:04.83 <FILTERING> Flow averaged 0.24ml/s over 30:38
@08:42:04.86 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi
@08:42:05.43 Relieved excess vacuum in .6 seconds
@08:42:05.45 SamplePump.seek 1000ml,46:34
@08:42:28.10 Flow averaged 0.20ml/s over 23 seconds
@08:42:28.13 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi
@08:42:28.70 Relieved excess vacuum in .6 seconds
@08:42:28.72 SamplePump.seek 1000ml,46:11
@08:42:29.63 <sampling> Cartridge 40 filtering 452/1000ml at 0.22ml/s with 24.5psi vacuum 3.2m deep (19.4psia)
@08:42:57.33 <FILTERING> Flow averaged 0.20ml/s over 29 seconds
@08:42:57.36 Waiting up to 14 seconds for vacuum to reduce from 28.3 to 18.0psi
@08:42:57.92 Relieved excess vacuum in .6 seconds
@08:42:57.95 SamplePump.seek 1000ml,45:42
@08:43:26.57 Flow averaged 0.20ml/s over 29 seconds
@08:43:26.60 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi
@08:43:27.16 Relieved excess vacuum in .6 seconds
@08:43:27.19 SamplePump.seek 1000ml,45:14
@08:43:29.63 <sampling> Cartridge 40 filtering 464/1000ml at 0.20ml/s with 21.7psi vacuum 4.2m deep (20.9psia)
@08:43:50.12 <FILTERING> Flow averaged 0.20ml/s over 23 seconds
@08:43:50.15 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi
@08:43:50.71 Relieved excess vacuum in .6 seconds
@08:43:50.74 SamplePump.seek 1000ml,44:50
@08:44:02.76 Flow averaged 0.20ml/s over 13 seconds
@08:44:02.78 <closeIntake> Intake.close
@08:44:02.82 <closeExhaust> Exhaust.close
@08:44:03.01 <FILTERING> Toroid.in :hold
@08:44:03.08 Toroid.seek :bypass
@08:44:04.71 Toroid.in :default
@08:44:04.77 Toroid.seek :clear
@08:44:06.38 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@08:44:06.39 Pressure.coast
@08:44:06.58 Sampled  470.7ml
@08:44:08.74 <LRAUV> -> Cmd.startProcessing
@08:44:09.11 <PROCESSING> Plunge.seek :reagent
@08:44:09.94 Sample loop closed. Ready to Deliver Reagents
@08:44:09.95 Archive.seek :sealed
@08:44:14.23 Archive.seek :evac
@08:44:28.18 delay 1:00
@08:45:28.21 Archive.seek :clear
@08:45:45.93 RNAlater soak for 10:00
@08:55:45.95 Archive.seek :tVent
@08:55:48.53 delay 1 second
@08:55:49.55 Archive.seek :evac
@08:56:05.18 delay 10 seconds
@08:56:15.21 Sample preserved and evacuated
@08:56:16.37 <LRAUV> -> Cmd.stop
@08:56:16.51 Carousel.seek 40
@08:56:16.68 Plunge.seek :locked
@08:56:17.63 <Archive> Archive.in :unlocking
@08:56:17.75 Archive.seek :clear
@08:56:17.82 <Air> Air.in :unlocking
@08:56:17.95 Air.seek :clear
@08:56:17.98 <Lysis1> Lysis1.in :unlocking
@08:56:18.14 Lysis1.seek :clear
@08:56:21.79 <LRAUV> Archive.coast
@08:56:21.84 Air.coast
@08:56:21.89 Lysis1.coast
@08:56:21.95 Lysis2.coast
@08:56:21.99 Plunge.coast
@08:56:22.04 SamplePump.coast
@08:56:22.09 Toroid.coast
@08:56:22.15 Carousel.coast
@08:56:22.20 PinPort.coast
@08:56:22.38 Gate.power :motor,:OFF
@08:56:22.40 Gate.power :core,:OFF
@08:56:22.42 Gate.power :analytic1,:OFF
@08:56:22.44 Gate.power :analytic2,:OFF
@08:56:22.46 Gate.power :analytic3,:OFF
@08:56:22.48 Gate.power :main,:OFF
@08:56:22.50 Safely stopped and ready to power off
##-- file closed 2021-04-22T16:08:59.454Z

##-- file opened 2021-04-22T16:08:59.456Z
@09:09:00.19 -> showStatus
@09:09:02.21 -> Cmd.loadCartridge -9
@09:09:02.35 Gate.power :main,:ON,limit to 5000mA
@09:09:02.37 Gate.power :analytic1,:ON,limit to 5000mA
@09:09:02.39 Gate.power :analytic2,:ON,limit to 5000mA
@09:09:02.41 Gate.power :analytic3,:ON,limit to 5000mA
@09:09:02.54 Powering core bus
@09:09:04.82 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@09:09:04.95 <LRAUV> Archive.configure :default
@09:09:05.07 Air.configure :default
@09:09:05.17 Lysis1.configure :default
@09:09:05.27 Lysis2.configure :default
@09:09:05.39 Plunge.configure :default
@09:09:05.52 SamplePump.configure :default
@09:09:05.67 ExtraPump.configure :default
@09:09:05.78 Toroid.configure :default
@09:09:05.90 Carousel.configure :default
@09:09:06.02 Pressure.configure :default
@09:09:06.13 PinPort.configure :default
@09:09:06.20 All elf controllers running firmware version 4.741
@09:09:06.23 Gate.power :motor,:ON,limit to 5000mA
@09:09:06.43 <Archive_home> Archive.in :default
@09:09:06.70 Archive.home.jog 0
@09:09:06.88 <Toroid_home> Toroid.in :default
@09:09:06.96 <Plunge_home> Plunge.in :default
@09:09:06.99 <Lysis1_home> Lysis1.in :default
@09:09:07.04 <PinPort_home> PinPort.in :default
@09:09:07.10 <Lysis2_home> Lysis2.in :default
@09:09:07.13 <Air_home> Air.in :default
@09:09:07.33 <Plunge_home> Plunge.home.jog 0
@09:09:07.36 <Toroid_home> Toroid.home.jog 0
@09:09:07.39 <Lysis1_home> Lysis1.home.jog 0
@09:09:07.41 <PinPort_home> PinPort.home.jog 0
@09:09:07.43 <Lysis2_home> Lysis2.home.jog 0
@09:09:07.46 <Air_home> Air.home.jog 0
@09:09:08.26 <Archive_home> Archive.in :homing
@09:09:08.55 <Toroid_home> Toroid.in :homing
@09:09:08.57 <Plunge_home> Plunge.in :homing
@09:09:08.60 <Air_home> Air.in :homing
@09:09:08.65 <PinPort_home> PinPort.in :homing
@09:09:08.68 <Lysis1_home> Lysis1.in :homing
@09:09:08.72 <Archive_home> Archive.jog 150
@09:09:08.76 <Lysis2_home> Lysis2.in :homing
@09:09:08.97 <Plunge_home> Plunge.jog 1574
@09:09:09.00 <PinPort_home> PinPort.jog -107
@09:09:09.03 <Toroid_home> Toroid.jog 611
@09:09:09.06 <Air_home> Air.jog 154
@09:09:09.09 <Lysis1_home> Lysis1.jog 1416
@09:09:09.12 <Lysis2_home> Lysis2.jog 151
@09:09:09.36 <Archive_home> Archive.setRawPosition! 50
@09:09:09.41 Archive.seek :home
@09:09:09.48 <PinPort_home> PinPort.setRawPosition! 0
@09:09:09.53 PinPort.seek :home
@09:09:09.61 PinPort.in :default
@09:09:09.72 <Air_home> Air.setRawPosition! 50
@09:09:09.75 <Archive_home> Archive.in :default
@09:09:09.81 <Air_home> Air.seek :home
@09:09:09.92 <Lysis2_home> Lysis2.setRawPosition! 50
@09:09:09.97 Lysis2.seek :home
@09:09:10.13 <Air_home> Air.in :default
@09:09:10.25 <Lysis2_home> Lysis2.in :default
@09:09:10.36 <Plunge_home> Plunge.setRawPosition! 50
@09:09:10.41 Plunge.seek :home
@09:09:10.50 <Lysis1_home> Lysis1.setRawPosition! 50
@09:09:10.54 Lysis1.seek :home
@09:09:10.69 <Plunge_home> Plunge.in :default
@09:09:10.82 <Lysis1_home> Lysis1.in :default
@09:09:11.24 <Toroid_home> Toroid.setRawPosition! 0
@09:09:11.29 Toroid.seek :home
@09:09:11.37 Toroid.in :default
@09:09:11.49 <LRAUV> Toroid.seek :clear
@09:09:13.11 Carousel.in :default
@09:09:13.17 Carousel.home.jog 0
@09:09:13.66 Carousel.in :default
@09:09:13.72 Carousel.seek 40
@09:09:13.85 Selecting Cartridge 37
@09:09:14.09 Carousel.seek 37
@09:09:14.99 Lysis1.in :fast
@09:09:15.05 Lysis1.seek :locked
@09:09:17.65 <Plunge> Plunge.seek :locked
@09:09:17.78 <Air> Air.in :fast
@09:09:17.84 Air.seek :locked
@09:09:20.45 <LRAUV> Powering cartridge bus
@09:09:22.57 Gate.setIR 1750,5000,0,6
@09:09:22.70 Gate.setIR 0,5000,0,6
@09:09:22.76 PuckHeater.configure :default
@09:09:22.80 Gate.power :heat,:ON,limit to 5000mA
@09:09:23.47 Mounted lysis Cartridge board#Q05060
@09:09:23.49 Using default puck heat loss curve for HeatingAccelInCarousel
@09:09:42.61 -> Cmd.startFiltering
@09:09:43.07 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@09:09:43.08 SamplePump.setPosition! 0ml
@09:09:43.12 Bypass.open
@09:09:44.60 Intake.open
@09:09:45.41 Sea 5.3m deep (22.5psia)
@09:09:45.43 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@09:09:48.01 Vacuum tare is 51% of full scale
@09:09:48.14 Pressure.in :default
@09:09:48.20 Pressure.hold
@09:09:48.37 SamplePump.in :prime
@09:09:48.43 SamplePump.seek 10ml,1:21
@09:09:57.82 Pressure.coast
@09:09:57.87 SamplePump.setPosition! 0ml
@09:09:57.92 Exhaust.open
@09:09:59.35 <closeBypass> Bypass.close
@09:10:00.87 <FILTERING> Pressure.in :default
@09:10:00.93 Pressure.hold
@09:10:01.04 Sea 6.3m deep (24.0psia)
@09:10:01.17 SamplePump.in :prime
@09:10:01.24 SamplePump.seek 100ml,8:51
@09:12:11.16 Pressure.coast
@09:12:11.22 Bypass.open
@09:12:11.92 Intake.close
@09:12:12.77 Toroid.in :hold
@09:12:12.83 Toroid.seek :crack
@09:12:14.39 Toroid.seek :cartridge
@09:12:15.49 Toroid.in :default
@09:12:15.55 Toroid.seek :clear
@09:12:17.50 Pressurizing cartridge to 26psi above 23.7psia
@09:12:17.52 SamplePump.setPosition! 0ml
@09:12:17.56 Intake.open
@09:12:18.28 Bypass.close
@09:12:19.03 Exhaust.close
@09:12:19.83 Pressure.in :default
@09:12:19.89 Pressure.hold
@09:12:20.06 SamplePump.in :charge
@09:12:20.12 SamplePump.seek 2.3ml,42 seconds
@09:12:29.12 Pressure.coast
@09:12:29.17 Intake.close
@09:12:30.00 Pressurized cartridge with 0.360ml to 26.2psi
@09:12:30.01 Isolated @46.1psia (22.4psi)
@09:12:30.02 Verifying cartridge holds at least 0.75..0.85 of 46.1psia
@09:12:30.05 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@09:12:34.67 Sampling 1000.0ml
@09:12:34.69 SamplePump.setPosition! 0ml
@09:12:34.74 Exhaust.open
@09:12:35.47 Intake.open
@09:12:36.34 Pressure.in :default
@09:12:36.40 Pressure.hold
@09:12:36.51 Sea 7.0m deep (25.1psia)
@09:12:36.65 SamplePump.in :sample
@09:12:36.71 SamplePump.seek 1000ml,1:23:53
@09:12:39.38 <sampling> Cartridge 37 filtering 1/1000ml at 0.35ml/s with 25.9psi vacuum 4.6m deep (21.5psia)
@09:13:39.37 Cartridge 37 filtering 19/1000ml at 0.28ml/s with 21.2psi vacuum 2.8m deep (18.8psia)
@09:14:39.38 Cartridge 37 filtering 38/1000ml at 0.33ml/s with 25.7psi vacuum 6.5m deep (24.3psia)
@09:15:39.37 Cartridge 37 filtering 55/1000ml at 0.25ml/s with 20.1psi vacuum 3.3m deep (19.5psia)
@09:16:39.38 Cartridge 37 filtering 73/1000ml at 0.26ml/s with 29.0psi vacuum
@09:17:39.37 Cartridge 37 filtering 89/1000ml at 0.29ml/s with 22.2psi vacuum 5.2m deep (22.3psia)
@09:18:39.38 Cartridge 37 filtering 106/1000ml at 0.31ml/s with 23.0psi vacuum 2.9m deep (19.0psia)
@09:19:39.78 Cartridge 37 filtering 122/1000ml at 0.28ml/s with 21.0psi vacuum 9.1m deep (28.2psia)
@09:20:39.38 Cartridge 37 filtering 138/1000ml at 0.28ml/s with 22.0psi vacuum 3.4m deep (19.7psia)
@09:21:39.37 Cartridge 37 filtering 153/1000ml at 0.21ml/s with 25.0psi vacuum 3.0m deep (19.1psia)
@09:22:39.38 Cartridge 37 filtering 168/1000ml at 0.29ml/s with 24.7psi vacuum 5.0m deep (22.1psia)
@09:23:39.37 Cartridge 37 filtering 183/1000ml at 0.26ml/s with 23.2psi vacuum 1.8m deep (17.4psia)
@09:24:39.38 Cartridge 37 filtering 198/1000ml at 0.21ml/s with 22.2psi vacuum 5.1m deep (22.2psia)
@09:25:39.37 Cartridge 37 filtering 212/1000ml at 0.20ml/s with 23.5psi vacuum
@09:26:39.38 Cartridge 37 filtering 226/1000ml at 0.21ml/s with 22.7psi vacuum 4.1m deep (20.7psia)
@09:27:39.37 Cartridge 37 filtering 240/1000ml at 0.24ml/s with 26.4psi vacuum 2.4m deep (18.3psia)
@09:28:39.38 Cartridge 37 filtering 253/1000ml at 0.20ml/s with 23.3psi vacuum 2.9m deep (19.0psia)
@09:29:39.37 Cartridge 37 filtering 266/1000ml at 0.21ml/s with 27.3psi vacuum 2.7m deep (18.7psia)
@09:30:39.38 Cartridge 37 filtering 280/1000ml at 0.20ml/s with 27.3psi vacuum
@09:31:39.37 Cartridge 37 filtering 293/1000ml at 0.24ml/s with 20.8psi vacuum 6.0m deep (23.5psia)
@09:32:39.38 Cartridge 37 filtering 305/1000ml at 0.23ml/s with 24.9psi vacuum 3.7m deep (20.1psia)
@09:33:39.37 Cartridge 37 filtering 318/1000ml at 0.21ml/s with 21.3psi vacuum 4.1m deep (20.7psia)
@09:34:39.38 Cartridge 37 filtering 330/1000ml at 0.20ml/s with 22.8psi vacuum 4.7m deep (21.6psia)
@09:35:39.37 Cartridge 37 filtering 343/1000ml at 0.20ml/s with 25.8psi vacuum
@09:36:39.38 Cartridge 37 filtering 355/1000ml at 0.21ml/s with 25.5psi vacuum 4.5m deep (21.3psia)
@09:37:39.37 Cartridge 37 filtering 367/1000ml at 0.20ml/s with 29.3psi vacuum 2.3m deep (18.2psia)
@09:37:42.76 <FILTERING> Flow averaged 0.24ml/s over 25:07
@09:37:42.79 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi
@09:37:43.36 Relieved excess vacuum in .6 seconds
@09:37:43.39 SamplePump.seek 1000ml,53:12
@09:37:52.40 Flow averaged 0.20ml/s over 10 seconds
@09:37:52.42 <closeIntake> Intake.close
@09:37:52.46 <closeExhaust> Exhaust.close
@09:37:52.65 <FILTERING> Toroid.in :hold
@09:37:52.71 Toroid.seek :bypass
@09:37:54.34 Toroid.in :default
@09:37:54.40 Toroid.seek :clear
@09:37:56.02 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@09:37:56.03 Pressure.coast
@09:37:56.21 Sampled  369.9ml
@09:37:58.26 <LRAUV> -> Cmd.startProcessing
@09:37:59.69 <PROCESSING> SPR.command \"read_sequences\"
@09:38:00.32 SPR.command \"stats_on\"
@09:38:00.36 SPR.command \"start 0 1\"
@09:38:02.37 <SPRlogger> Begun log /var/log/esp/spr21apr2209h.spr
@09:38:03.17 <PROCESSING> Handoff.select :waste
@09:38:09.85 Syringe tare is 19% of full scale
@09:38:09.87 PinPort.in :locking
@09:38:09.94 PinPort.seek :locked
@09:38:10.42 <posArchive> Archive.in :fast
@09:38:10.46 <posLyS2> Lysis2.in :fast
@09:38:10.56 <PROCESSING> Lysis1.seek 1350uL
@09:38:10.61 <posLyS2> Lysis2.seek :full
@09:38:10.64 <posArchive> Archive.seek :full
@09:38:12.75 <PROCESSING> Plunge.seek :reagent
@09:38:13.65 Evacuating puck with filter bubble point of 50psi
@09:38:13.67 PinPort.seek :locked
@09:38:13.75 Handoff.select :waste
@09:38:20.34 Air syringe pumping up to 1 stroke to reach 8psi
@09:38:20.35 Air stroke #1 of 1
@09:38:20.43 Air.in :pressurize
@09:38:20.49 Air.seek :pressurized
@09:38:31.74 -0.0psi after pressurize
@09:38:31.75 Air.in :compress
@09:38:31.82 Air.seek :evac
@09:38:35.91 0.6psi after compress
@09:38:48.48 0.2psi after compress (stable)
@09:38:48.49 Air.in :default
@09:38:48.56 Air.hold
@09:38:48.74 at 0.2psi Opened to waste
@09:38:48.75 Handoff.select :blocked
@09:38:48.88 Archive syringe pumping up to 5 strokes to reach 50psi
@09:38:48.89 Archive stroke #1 of 5
@09:38:48.96 Archive.in :pressurize
@09:38:49.03 Archive.seek :pressurized
@09:39:01.78 9.4psi after pressurize
@09:39:01.79 Archive.in :compress
@09:39:01.86 Archive.seek :empty
@09:39:17.75 38.3psi after compress
@09:39:30.20 Reached 50.0 of 50.0psi after pumping 1730uL (1.00 strokes)
@09:39:30.21 Archive.in :default
@09:39:30.28 Archive.hold
@09:39:30.34 Handoff.select :waste
@09:39:40.52 delay 1:00
@09:40:40.55 Handoff.select :blocked
@09:41:03.23 Handoff.select :waste
@09:41:03.36 1.7psi at evacEnd
@09:41:03.37 Handoff.select :blocked
@09:41:03.49 Archive.in :fast
@09:41:03.56 Archive.seek :topVent
@09:41:17.26 Stable @2.4psi after Handoff blocked
@09:41:17.28 Archive syringe pumping up to 5 strokes to reach 32psi
@09:41:17.29 Archive stroke #1 of 5
@09:41:17.39 Archive.in :pressurize
@09:41:17.45 Archive.seek :pressurized
@09:41:30.18 3.8psi after pressurize
@09:41:30.19 Archive.in :compress
@09:41:30.27 Archive.seek :empty
@09:41:46.15 5.7psi after compress
@09:42:33.44 Reached 32.5 of 32.4psi after pumping 1730uL (1.00 strokes)
@09:42:33.46 Archive.in :default
@09:42:33.53 Archive.hold
@09:42:33.62 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position
@09:42:33.64 Handoff.select :waste
@09:42:43.33 delay 1:00
@09:43:43.35 Handoff.select :blocked
@09:44:06.52 Handoff.select :waste
@09:44:06.64 4.8psi at evacEnd
@09:44:06.66 Air.seek :fullVent
@09:44:08.10 Archive.in :fast
@09:44:08.16 Archive.seek :topVent
@09:44:15.29 Seawater evacuated, Presure above/below filter released. Lysis begins
@09:44:15.39 Lysis1.in :slow
@09:44:15.46 Lysis1.seek 1050uL
@09:44:45.47 delay 5 seconds
@09:44:50.49 Handoff.select :blocked
@09:44:50.65 Lysis1.seek 450uL
@09:45:50.60 delay 3 seconds
@09:45:53.67 Air.in to mix into 1.37806 parts Lysis1
@09:45:54.26 <Airmixer> Air.seek :evac
@09:45:54.30 <PROCESSING> Lysis1.seek 300uL
@09:46:09.40 Lysis1.seek :empty
@09:46:39.48 Lysis buffer delivered @34.7psi
@09:46:39.49 Polling Can every 2:00
@09:46:40.05 PuckHeater.configure :default #ambient 11.6C
@09:46:40.11 PuckHeater.seek 80.2C
@09:47:55.91 PuckHeater.hold 2:00,80.2C
@09:49:55.70 Polling Can every 2:00
@09:49:56.25 PuckHeater.configure :default #ambient 11.7C
@09:49:56.29 PuckHeater.hold 30 seconds,79.2C
@09:50:26.39 Polling Can every 2:00
@09:50:26.94 PuckHeater.configure :default #ambient 11.7C
@09:50:26.99 PuckHeater.hold 30 seconds,78.6C
@09:50:57.11 Polling Can every 2:00
@09:50:57.66 PuckHeater.configure :default #ambient 11.7C
@09:50:57.71 PuckHeater.hold 10:00,78.1C
@10:00:56.35 Lysis complete. Syringe pressure @35.7psi
@10:00:56.36 Handoff.select :blocked
@10:00:56.40 Archive syringe pumping up to 3 strokes to reach 40psi
@10:00:56.41 Archive stroke #1 of 3
@10:00:56.50 Archive.in :pressurize
@10:00:56.56 Archive.seek :pressurized
@10:01:09.31 35.3psi after pressurize
@10:01:09.32 Archive.in :compress
@10:01:09.39 Archive.seek :empty
@10:01:17.95 Reached 42.0 of 40.0psi after pumping 1573uL (0.91 strokes)
@10:01:17.96 Archive.in :default
@10:01:18.03 Archive.hold
@10:01:18.09 delay 2 seconds
@10:01:20.11 Archive.in :fast
@10:01:20.17 Archive.seek :full
@10:01:27.21 Lysis1.seek 138uL
@10:01:41.10 delay 1 second
@10:01:42.13 <fillArchive> Archive.seek :full
@10:01:42.17 <PROCESSING> Lysis1.seek :empty
@10:01:56.13 Initial air pressure @51.7psi
@10:01:56.20 Lysis1.in :extract
@10:01:56.28 Lysis1.seek 600uL,nil
@10:01:58.56 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@10:01:58.57 Archive stroke #1 of 6
@10:01:58.70 Archive.in :pressurize
@10:01:58.76 Archive.seek :pressurized
@10:02:11.49 15.5psi after pressurize
@10:02:11.50 Archive.in :compress
@10:02:11.57 Archive.seek :empty
@10:02:27.45 16.2psi after compress
@10:02:30.54 Archive.in :default
@10:02:30.59 Archive.hold
@10:02:30.72 <PROCESSING> Initial 600uL lysate @16.0psi
@10:02:30.74 Handoff.select :blocked
@10:02:30.83 Initial air pressure @16.3psi
@10:02:30.91 Lysis1.seek 825uL,nil
@10:02:32.09 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@10:02:32.10 Archive stroke #1 of 6
@10:02:32.18 Archive.in :compress
@10:02:32.25 Archive.seek :empty
@10:02:32.42 14.4psi after compress
@10:02:45.03 15.7psi after compress (stable)
@10:02:50.16 Recovering 4.4uL/s
@10:02:55.28 Recovering 2.4uL/s
@10:03:00.34 Recovering 3.0uL/s
@10:03:05.41 Archive.in :gulp
@10:03:05.47 Archive.seek :topVent
@10:03:12.70 15.4psi after gulp
@10:03:25.76 15.7psi after gulp (stable)
@10:03:25.77 Archive stroke #2 of 6
@10:03:25.92 Archive.in :pressurize
@10:03:26.02 Archive.seek :pressurized
@10:03:38.78 15.9psi after pressurize
@10:03:38.80 Archive.in :compress
@10:03:38.87 Archive.seek :empty
@10:03:50.40 Archive.in :default
@10:03:50.45 Archive.hold
@10:03:50.75 <PROCESSING> Recovered 825uL lysate @15.8psi
@10:03:50.76 Air.in :halfSpeed
@10:03:50.83 Air.seek :fullVent
@10:03:53.07 Puck Top Vented, bottom @18.7psi
@10:03:53.08 Handoff.select :waste
@10:03:53.20 delay 5 seconds
@10:03:58.26 Handoff open to waste, bottom @0.4psi
@10:03:58.28 Air.in :default
@10:03:58.34 Air.seek :evac
@10:03:59.78 Lysis2.in :DAmix
@10:03:59.88 Lysis1.in :DAmix
@10:04:00.36 <Lysis2mixer> Lysis2.seek 1480uL
@10:04:00.39 <PROCESSING> Lysis1.seek 575uL
@10:04:35.48 delay 5 seconds
@10:04:40.51 Handoff.select SPR
@10:04:40.62 SPR.command \"start 2 14\"
@10:04:41.73 delay 10 seconds
@10:04:51.75 SPR.command \"start 1 0\"
@10:04:54.99 Waiting up to 4:00 for SPR to enter state 1
@10:06:57.36 SPR.command \"hold_on\"
@10:06:58.77 SPR Dilution 1 of 1 -- Running Experiment 1
@10:06:59.21 <Lysis2mixer> Lysis2.seek 1180uL
@10:06:59.26 <PROCESSING> Lysis1.seek 275uL
@10:07:41.29 volL1 = 300.0 and volL2 = 300.0
@10:07:41.31 delay 15 seconds
@10:07:56.33 SPR.command \"hold_off\"
@10:07:59.43 Waiting up to 38:00 for SPR to enter state 7
@10:34:42.97 SPR Analysis has Completed
@10:34:42.98 Handoff.select :waste
@10:34:43.91 <SPRlogger> Ended log /var/log/esp/spr21apr2209h.spr
@10:34:43.92 <PROCESSING> SPR.command \"stats_off\"
@10:34:45.33 <LRAUV> -> Cmd.stop
@10:34:45.35 Archive.stop
@10:34:45.47 <closeHandoff> Handoff.select :close
@10:34:45.60 <LRAUV> Gate.power :heat,:OFF
@10:34:45.62 Gate.power :cartridge,:OFF
@10:34:45.70 Carousel.seek 37
@10:34:45.87 Plunge.seek :locked
@10:34:46.76 PinPort.in :unlocking
@10:34:46.82 PinPort.seek :clear
@10:34:47.36 <Archive> Archive.in :unlocking
@10:34:47.49 Archive.seek :clear
@10:34:47.52 <Air> Air.in :unlocking
@10:34:47.67 Air.seek :clear
@10:34:47.71 <Lysis1> Lysis1.in :unlocking
@10:34:47.85 Lysis1.seek :clear
@10:34:47.95 <Lysis2> Lysis2.in :unlocking
@10:34:48.02 Lysis2.seek :clear
@10:34:51.49 <LRAUV> Archive.coast
@10:34:51.54 Air.coast
@10:34:51.59 Lysis1.coast
@10:34:51.64 Lysis2.coast
@10:34:51.69 Plunge.coast
@10:34:51.74 SamplePump.coast
@10:34:51.79 Toroid.coast
@10:34:51.85 Carousel.coast
@10:34:51.89 PinPort.coast
@10:34:52.07 Gate.power :motor,:OFF
@10:34:52.08 Gate.power :core,:OFF
@10:34:52.11 Gate.power :analytic1,:OFF
@10:34:52.13 Gate.power :analytic2,:OFF
@10:34:52.15 Gate.power :analytic3,:OFF
@10:34:52.17 Gate.power :main,:OFF
@10:34:52.19 Safely stopped and ready to power off
##-- file closed 2021-04-22T19:41:44.930Z

##-- file opened 2021-04-22T19:41:44.931Z
@12:41:45.35 -> showStatus
@12:41:47.37 -> Cmd.loadCartridge -6
@12:41:47.51 Gate.power :main,:ON,limit to 5000mA
@12:41:47.53 Gate.power :analytic1,:ON,limit to 5000mA
@12:41:47.55 Gate.power :analytic2,:ON,limit to 5000mA
@12:41:47.58 Gate.power :analytic3,:ON,limit to 5000mA
@12:41:47.71 Powering core bus
@12:41:49.99 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@12:41:50.12 <LRAUV> Archive.configure :default
@12:41:50.24 Air.configure :default
@12:41:50.34 Lysis1.configure :default
@12:41:50.45 Lysis2.configure :default
@12:41:50.57 Plunge.configure :default
@12:41:50.70 SamplePump.configure :default
@12:41:50.85 ExtraPump.configure :default
@12:41:50.97 Toroid.configure :default
@12:41:51.09 Carousel.configure :default
@12:41:51.21 Pressure.configure :default
@12:41:51.32 PinPort.configure :default
@12:41:51.39 All elf controllers running firmware version 4.741
@12:41:51.42 Gate.power :motor,:ON,limit to 5000mA
@12:41:51.63 <Archive_home> Archive.in :default
@12:41:51.89 Archive.home.jog 0
@12:41:52.06 <Toroid_home> Toroid.in :default
@12:41:52.14 <Plunge_home> Plunge.in :default
@12:41:52.17 <Lysis1_home> Lysis1.in :default
@12:41:52.21 <Air_home> Air.in :default
@12:41:52.25 <PinPort_home> PinPort.in :default
@12:41:52.28 <Lysis2_home> Lysis2.in :default
@12:41:52.51 <Toroid_home> Toroid.home.jog 0
@12:41:52.55 <Lysis1_home> Lysis1.home.jog 0
@12:41:52.57 <Plunge_home> Plunge.home.jog 0
@12:41:52.60 <PinPort_home> PinPort.home.jog 0
@12:41:52.65 <Air_home> Air.home.jog 0
@12:41:52.68 <Lysis2_home> Lysis2.home.jog 0
@12:41:53.44 <Archive_home> Archive.in :homing
@12:41:53.60 <Toroid_home> Toroid.in :homing
@12:41:53.64 <Plunge_home> Plunge.in :homing
@12:41:53.69 <Archive_home> Archive.jog 152
@12:41:53.74 <Lysis1_home> Lysis1.in :homing
@12:41:53.77 <PinPort_home> PinPort.in :homing
@12:41:53.99 PinPort.jog -107
@12:41:54.03 <Plunge_home> Plunge.jog 1577
@12:41:54.05 <Lysis1_home> Lysis1.jog 1419
@12:41:54.08 <Toroid_home> Toroid.jog 624
@12:41:54.28 <Air_home> Air.in :homing
@12:41:54.33 <Lysis2_home> Lysis2.in :homing
@12:41:54.40 <Air_home> Air.jog 172
@12:41:54.45 <Lysis2_home> Lysis2.jog 1431
@12:41:54.55 <Archive_home> Archive.setRawPosition! 50
@12:41:54.57 <PinPort_home> PinPort.setRawPosition! 0
@12:41:54.66 <Archive_home> Archive.seek :home
@12:41:54.68 <PinPort_home> PinPort.seek :home
@12:41:54.82 PinPort.in :default
@12:41:54.90 <Air_home> Air.setRawPosition! 50
@12:41:54.95 Air.seek :home
@12:41:55.05 <Archive_home> Archive.in :default
@12:41:55.23 <Air_home> Air.in :default
@12:41:55.31 <Lysis1_home> Lysis1.setRawPosition! 50
@12:41:55.38 Lysis1.seek :home
@12:41:55.46 <Plunge_home> Plunge.setRawPosition! 50
@12:41:55.51 Plunge.seek :home
@12:41:55.66 <Lysis2_home> Lysis2.setRawPosition! 50
@12:41:55.71 Lysis2.seek :home
@12:41:55.81 <Lysis1_home> Lysis1.in :default
@12:41:55.84 <Plunge_home> Plunge.in :default
@12:41:55.99 <Lysis2_home> Lysis2.in :default
@12:41:56.28 <Toroid_home> Toroid.setRawPosition! 0
@12:41:56.33 Toroid.seek :home
@12:41:56.42 Toroid.in :default
@12:41:56.54 <LRAUV> Toroid.seek :clear
@12:41:58.17 Carousel.in :default
@12:41:58.23 Carousel.home.jog 0
@12:41:58.72 Carousel.in :default
@12:41:58.78 Carousel.seek 37
@12:41:58.92 Selecting Cartridge 38
@12:41:59.16 Carousel.seek 38
@12:41:59.64 Lysis1.in :fast
@12:41:59.70 Lysis1.seek :locked
@12:42:02.29 <Plunge> Plunge.seek :locked
@12:42:02.41 <Air> Air.in :fast
@12:42:02.48 Air.seek :locked
@12:42:05.08 <LRAUV> Powering cartridge bus
@12:42:07.20 Gate.setIR 1750,5000,0,6
@12:42:07.33 Gate.setIR 0,5000,0,6
@12:42:07.38 Gate.power :cartridge,:OFF
@12:42:07.92 Mounted archive Cartridge
@12:42:26.96 -> Cmd.startFiltering
@12:42:27.41 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@12:42:27.42 SamplePump.setPosition! 0ml
@12:42:27.47 Bypass.open
@12:42:28.97 Intake.open
@12:42:29.72 Sea 6.3m deep (24.0psia)
@12:42:29.73 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@12:42:32.31 Vacuum tare is 51% of full scale
@12:42:32.44 Pressure.in :default
@12:42:32.50 Pressure.hold
@12:42:32.66 SamplePump.in :prime
@12:42:32.73 SamplePump.seek 10ml,1:21
@12:42:42.13 Pressure.coast
@12:42:42.17 SamplePump.setPosition! 0ml
@12:42:42.22 Exhaust.open
@12:42:43.66 <closeBypass> Bypass.close
@12:42:45.25 <FILTERING> Pressure.in :default
@12:42:45.31 Pressure.hold
@12:42:45.43 Sea 7.0m deep (25.0psia)
@12:42:45.56 SamplePump.in :prime
@12:42:45.63 SamplePump.seek 100ml,8:51
@12:44:55.45 Pressure.coast
@12:44:55.51 Bypass.open
@12:44:56.23 Intake.close
@12:44:57.07 Toroid.in :hold
@12:44:57.13 Toroid.seek :crack
@12:44:58.69 Toroid.seek :cartridge
@12:44:59.78 Toroid.in :default
@12:44:59.85 Toroid.seek :clear
@12:45:01.81 Pressurizing cartridge to 26psi above 22.6psia
@12:45:01.82 SamplePump.setPosition! 0ml
@12:45:01.87 Intake.open
@12:45:02.62 Bypass.close
@12:45:03.32 Exhaust.close
@12:45:04.16 Pressure.in :default
@12:45:04.22 Pressure.hold
@12:45:04.39 SamplePump.in :charge
@12:45:04.45 SamplePump.seek 2.3ml,42 seconds
@12:45:10.44 Pressure.coast
@12:45:10.49 Intake.close
@12:45:11.34 Pressurized cartridge with 0.157ml to 26.6psi
@12:45:11.35 Isolated @44.4psia (21.8psi)
@12:45:11.36 Verifying cartridge holds at least 0.75..0.85 of 44.4psia
@12:45:11.39 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@12:45:16.01 Sampling 1000.0ml
@12:45:16.03 SamplePump.setPosition! 0ml
@12:45:16.08 Exhaust.open
@12:45:16.80 Intake.open
@12:45:17.68 Pressure.in :default
@12:45:17.74 Pressure.hold
@12:45:17.85 Sea 6.4m deep (24.1psia)
@12:45:17.98 SamplePump.in :sample
@12:45:18.05 SamplePump.seek 1000ml,1:23:53
@12:45:20.71 <sampling> Cartridge 38 filtering 1/1000ml at 0.34ml/s with 25.7psi vacuum 4.8m deep (21.8psia)
@12:46:20.71 Cartridge 38 filtering 19/1000ml at 0.34ml/s with 21.7psi vacuum 5.0m deep (22.1psia)
@12:47:20.71 Cartridge 38 filtering 37/1000ml at 0.33ml/s with 23.3psi vacuum 1.8m deep (17.4psia)
@12:48:20.71 Cartridge 38 filtering 55/1000ml at 0.27ml/s with 18.5psi vacuum 5.7m deep (23.1psia)
@12:49:20.71 Cartridge 38 filtering 72/1000ml at 0.30ml/s with 24.4psi vacuum
@12:50:20.71 Cartridge 38 filtering 88/1000ml at 0.30ml/s with 23.8psi vacuum
@12:51:20.71 Cartridge 38 filtering 105/1000ml at 0.29ml/s with 25.0psi vacuum 4.7m deep (21.6psia)
@12:52:20.71 Cartridge 38 filtering 121/1000ml at 0.28ml/s with 24.5psi vacuum 2.6m deep (18.5psia)
@12:53:21.13 Cartridge 38 filtering 137/1000ml at 0.27ml/s with 22.5psi vacuum 5.5m deep (22.8psia)
@12:54:20.71 Cartridge 38 filtering 152/1000ml at 0.27ml/s with 23.0psi vacuum 4.6m deep (21.5psia)
@12:55:20.71 Cartridge 38 filtering 168/1000ml at 0.29ml/s with 23.3psi vacuum 2.2m deep (18.0psia)
@12:56:20.71 Cartridge 38 filtering 183/1000ml at 0.21ml/s with 22.1psi vacuum 4.0m deep (20.6psia)
@12:57:20.71 Cartridge 38 filtering 197/1000ml at 0.26ml/s with 27.9psi vacuum 2.4m deep (18.3psia)
@12:58:20.71 Cartridge 38 filtering 212/1000ml at 0.27ml/s with 27.1psi vacuum 2.8m deep (18.8psia)
@12:59:20.71 Cartridge 38 filtering 226/1000ml at 0.20ml/s with 22.1psi vacuum 4.4m deep (21.2psia)
@13:00:20.71 Cartridge 38 filtering 241/1000ml at 0.24ml/s with 27.8psi vacuum 1.7m deep (17.2psia)
@13:01:20.71 Cartridge 38 filtering 254/1000ml at 0.24ml/s with 28.5psi vacuum
@13:02:20.71 Cartridge 38 filtering 268/1000ml at 0.23ml/s with 24.6psi vacuum 3.6m deep (20.0psia)
@13:03:20.71 Cartridge 38 filtering 282/1000ml at 0.21ml/s with 23.9psi vacuum 2.0m deep (17.7psia)
@13:04:20.71 Cartridge 38 filtering 295/1000ml at 0.23ml/s with 24.3psi vacuum 3.7m deep (20.2psia)
@13:05:20.71 Cartridge 38 filtering 309/1000ml at 0.20ml/s with 25.0psi vacuum 4.5m deep (21.4psia)
@13:06:20.71 Cartridge 38 filtering 322/1000ml at 0.22ml/s with 24.1psi vacuum 2.3m deep (18.1psia)
@13:07:20.71 Cartridge 38 filtering 335/1000ml at 0.20ml/s with 27.2psi vacuum 3.0m deep (19.2psia)
@13:08:20.71 Cartridge 38 filtering 348/1000ml at 0.20ml/s with 25.1psi vacuum 3.3m deep (19.6psia)
@13:09:20.71 Cartridge 38 filtering 360/1000ml at 0.20ml/s with 27.6psi vacuum
@13:10:20.71 Cartridge 38 filtering 373/1000ml at 0.21ml/s with 24.3psi vacuum 4.8m deep (21.9psia)
@13:11:20.71 Cartridge 38 filtering 385/1000ml at 0.20ml/s with 24.9psi vacuum 3.6m deep (20.0psia)
@13:12:20.71 Cartridge 38 filtering 398/1000ml at 0.20ml/s with 24.9psi vacuum
@13:13:20.71 Cartridge 38 filtering 410/1000ml at 0.20ml/s with 30.2psi vacuum 4.1m deep (20.7psia)
@13:14:20.71 Cartridge 38 filtering 422/1000ml at 0.20ml/s with 24.0psi vacuum 4.2m deep (20.9psia)
@13:15:03.44 <FILTERING> Flow averaged 0.24ml/s over 29:46
@13:15:03.47 Waiting up to 13 seconds for vacuum to reduce from 27.8 to 18.0psi
@13:15:04.04 Relieved excess vacuum in .6 seconds
@13:15:04.07 SamplePump.seek 1000ml,47:56
@13:15:20.71 <sampling> Cartridge 38 filtering 435/1000ml at 0.20ml/s with 29.2psi vacuum
@13:15:59.52 <FILTERING> Flow averaged 0.20ml/s over 56 seconds
@13:15:59.55 Waiting up to 14 seconds for vacuum to reduce from 28.6 to 18.0psi
@13:16:00.13 Relieved excess vacuum in .6 seconds
@13:16:00.15 SamplePump.seek 1000ml,47:00
@13:16:20.71 <sampling> Cartridge 38 filtering 447/1000ml at 0.21ml/s with 24.7psi vacuum 5.2m deep (22.4psia)
@13:16:39.32 <FILTERING> Flow averaged 0.20ml/s over 40 seconds
@13:16:39.35 Waiting up to 11 seconds for vacuum to reduce from 27.0 to 18.0psi
@13:16:39.93 Relieved excess vacuum in .6 seconds
@13:16:39.95 SamplePump.seek 1000ml,46:20
@13:16:47.68 Flow estimate reduced to 0.16ml/s
@13:16:47.71 <closeIntake> Intake.close
@13:16:47.76 <closeExhaust> Exhaust.close
@13:16:47.94 <FILTERING> Toroid.in :hold
@13:16:48.01 Toroid.seek :bypass
@13:16:49.64 Toroid.in :default
@13:16:49.70 Toroid.seek :clear
@13:16:51.31 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@13:16:51.32 Pressure.coast
@13:16:51.51 Sampled  451.9ml
@13:16:53.50 <LRAUV> -> Cmd.startProcessing
@13:16:53.87 <PROCESSING> Plunge.seek :reagent
@13:16:54.71 Sample loop closed. Ready to Deliver Reagents
@13:16:54.72 Archive.seek :sealed
@13:16:59.00 Archive.seek :evac
@13:17:12.95 delay 1:00
@13:18:12.97 Archive.seek :clear
@13:18:30.71 RNAlater soak for 10:00
@13:28:30.74 Archive.seek :tVent
@13:28:33.32 delay 1 second
@13:28:34.34 Archive.seek :evac
@13:28:49.97 delay 10 seconds
@13:29:00.00 Sample preserved and evacuated
@13:29:01.14 <LRAUV> -> Cmd.stop
@13:29:01.28 Carousel.seek 38
@13:29:01.45 Plunge.seek :locked
@13:29:02.39 <Archive> Archive.in :unlocking
@13:29:02.52 Archive.seek :clear
@13:29:02.56 <Air> Air.in :unlocking
@13:29:02.71 Air.seek :clear
@13:29:02.74 <Lysis1> Lysis1.in :unlocking
@13:29:02.90 Lysis1.seek :clear
@13:29:06.59 <LRAUV> Archive.coast
@13:29:06.64 Air.coast
@13:29:06.69 Lysis1.coast
@13:29:06.75 Lysis2.coast
@13:29:06.79 Plunge.coast
@13:29:06.84 SamplePump.coast
@13:29:06.90 Toroid.coast
@13:29:06.95 Carousel.coast
@13:29:07.00 PinPort.coast
@13:29:07.18 Gate.power :motor,:OFF
@13:29:07.20 Gate.power :core,:OFF
@13:29:07.22 Gate.power :analytic1,:OFF
@13:29:07.24 Gate.power :analytic2,:OFF
@13:29:07.26 Gate.power :analytic3,:OFF
@13:29:07.28 Gate.power :main,:OFF
@13:29:07.30 Safely stopped and ready to power off
##-- file closed 2021-04-22T20:41:01.409Z

##-- file opened 2021-04-22T20:41:01.411Z
@13:41:02.18 -> showStatus
@13:41:04.20 -> Cmd.loadCartridge -9
@13:41:04.34 Gate.power :main,:ON,limit to 5000mA
@13:41:04.36 Gate.power :analytic1,:ON,limit to 5000mA
@13:41:04.38 Gate.power :analytic2,:ON,limit to 5000mA
@13:41:04.40 Gate.power :analytic3,:ON,limit to 5000mA
@13:41:04.53 Powering core bus
@13:41:06.82 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@13:41:06.96 <LRAUV> Archive.configure :default
@13:41:07.08 Air.configure :default
@13:41:07.18 Lysis1.configure :default
@13:41:07.29 Lysis2.configure :default
@13:41:07.41 Plunge.configure :default
@13:41:07.53 SamplePump.configure :default
@13:41:07.68 ExtraPump.configure :default
@13:41:07.79 Toroid.configure :default
@13:41:07.92 Carousel.configure :default
@13:41:08.05 Pressure.configure :default
@13:41:08.16 PinPort.configure :default
@13:41:08.22 All elf controllers running firmware version 4.741
@13:41:08.25 Gate.power :motor,:ON,limit to 5000mA
@13:41:08.53 <Archive_home> Archive.in :default
@13:41:08.62 <Lysis1_home> Lysis1.in :default
@13:41:08.70 <Air_home> Air.in :default
@13:41:08.85 <Archive_home> Archive.home.jog 0
@13:41:08.90 <Lysis1_home> Lysis1.home.jog 0
@13:41:08.93 <Air_home> Air.home.jog 0
@13:41:09.12 <Toroid_home> Toroid.in :default
@13:41:09.20 <Plunge_home> Plunge.in :default
@13:41:09.31 <PinPort_home> PinPort.in :default
@13:41:09.36 <Lysis2_home> Lysis2.in :default
@13:41:09.49 <Toroid_home> Toroid.home.jog 0
@13:41:09.52 <PinPort_home> PinPort.home.jog 0
@13:41:09.91 <Plunge_home> Plunge.home.jog 0
@13:41:09.95 <Lysis2_home> Lysis2.home.jog 0
@13:41:10.28 <Air_home> Air.in :homing
@13:41:10.37 <Archive_home> Archive.in :homing
@13:41:10.40 <Lysis1_home> Lysis1.in :homing
@13:41:10.51 <Air_home> Air.jog 155
@13:41:10.54 <Lysis1_home> Lysis1.jog 1418
@13:41:10.58 <Archive_home> Archive.jog 151
@13:41:10.76 <Toroid_home> Toroid.in :homing
@13:41:10.79 <Plunge_home> Plunge.in :homing
@13:41:10.82 <PinPort_home> PinPort.in :homing
@13:41:10.86 <Lysis2_home> Lysis2.in :homing
@13:41:10.98 <Plunge_home> Plunge.jog 1574
@13:41:11.06 <PinPort_home> PinPort.jog -106
@13:41:11.08 <Lysis2_home> Lysis2.jog 1429
@13:41:11.13 <Toroid_home> Toroid.jog 611
@13:41:11.29 <Archive_home> Archive.setRawPosition! 50
@13:41:11.31 <Air_home> Air.setRawPosition! 50
@13:41:11.41 <Archive_home> Archive.seek :home
@13:41:11.43 <Air_home> Air.seek :home
@13:41:11.55 <PinPort_home> PinPort.setRawPosition! 0
@13:41:11.60 PinPort.seek :home
@13:41:11.69 PinPort.in :default
@13:41:11.80 <Lysis1_home> Lysis1.setRawPosition! 50
@13:41:11.84 <Archive_home> Archive.in :default
@13:41:11.86 <Air_home> Air.in :default
@13:41:11.95 <Lysis1_home> Lysis1.seek :home
@13:41:12.27 Lysis1.in :default
@13:41:12.37 <Lysis2_home> Lysis2.setRawPosition! 50
@13:41:12.40 <Plunge_home> Plunge.setRawPosition! 50
@13:41:12.46 <Lysis2_home> Lysis2.seek :home
@13:41:12.50 <Plunge_home> Plunge.seek :home
@13:41:12.79 <Lysis2_home> Lysis2.in :default
@13:41:12.87 <Plunge_home> Plunge.in :default
@13:41:13.29 <Toroid_home> Toroid.setRawPosition! 0
@13:41:13.34 Toroid.seek :home
@13:41:13.42 Toroid.in :default
@13:41:13.55 <LRAUV> Toroid.seek :clear
@13:41:15.16 Carousel.in :default
@13:41:15.22 Carousel.home.jog 0
@13:41:15.71 Carousel.in :default
@13:41:15.77 Carousel.seek 38
@13:41:15.92 Selecting Cartridge 35
@13:41:16.17 Carousel.seek 35
@13:41:17.06 Lysis1.in :fast
@13:41:17.12 Lysis1.seek :locked
@13:41:19.72 <Plunge> Plunge.seek :locked
@13:41:19.84 <Air> Air.in :fast
@13:41:19.91 Air.seek :locked
@13:41:22.52 <LRAUV> Powering cartridge bus
@13:41:24.64 Gate.setIR 1750,5000,0,6
@13:41:24.77 Gate.setIR 0,5000,0,6
@13:41:24.83 PuckHeater.configure :default
@13:41:24.87 Gate.power :heat,:ON,limit to 5000mA
@13:41:25.54 Mounted lysis Cartridge board#Q05092
@13:41:25.57 Using default puck heat loss curve for HeatingAccelInCarousel
@13:41:44.60 -> Cmd.startFiltering
@13:41:45.05 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@13:41:45.06 SamplePump.setPosition! 0ml
@13:41:45.11 Bypass.open
@13:41:46.56 Intake.open
@13:41:47.34 Sea 4.8m deep (21.8psia)
@13:41:47.36 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@13:41:49.94 Vacuum tare is 51% of full scale
@13:41:50.06 Pressure.in :default
@13:41:50.13 Pressure.hold
@13:41:50.30 SamplePump.in :prime
@13:41:50.37 SamplePump.seek 10ml,1:21
@13:41:59.76 Pressure.coast
@13:41:59.81 SamplePump.setPosition! 0ml
@13:41:59.86 Exhaust.open
@13:42:01.29 <closeBypass> Bypass.close
@13:42:02.83 <FILTERING> Pressure.in :default
@13:42:02.89 Pressure.hold
@13:42:03.00 Sea 5.4m deep (22.6psia)
@13:42:03.13 SamplePump.in :prime
@13:42:03.20 SamplePump.seek 100ml,8:51
@13:44:12.87 Pressure.coast
@13:44:12.93 Bypass.open
@13:44:13.67 Intake.close
@13:44:14.54 Toroid.in :hold
@13:44:14.60 Toroid.seek :crack
@13:44:16.15 Toroid.seek :cartridge
@13:44:17.25 Toroid.in :default
@13:44:17.31 Toroid.seek :clear
@13:44:19.27 Pressurizing cartridge to 26psi above 22.5psia
@13:44:19.28 SamplePump.setPosition! 0ml
@13:44:19.32 Intake.open
@13:44:20.08 Bypass.close
@13:44:20.83 Exhaust.close
@13:44:21.62 Pressure.in :default
@13:44:21.68 Pressure.hold
@13:44:21.85 SamplePump.in :charge
@13:44:21.92 SamplePump.seek 2.3ml,42 seconds
@13:44:31.55 Pressure.coast
@13:44:31.61 Intake.close
@13:44:32.44 Pressurized cartridge with 0.413ml to 27.0psi
@13:44:32.45 Isolated @44.1psia (21.6psi)
@13:44:32.47 Verifying cartridge holds at least 0.75..0.85 of 44.1psia
@13:44:32.50 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@13:44:37.12 Sampling 1000.0ml
@13:44:37.14 SamplePump.setPosition! 0ml
@13:44:37.19 Exhaust.open
@13:44:37.89 Intake.open
@13:44:38.76 Pressure.in :default
@13:44:38.82 Pressure.hold
@13:44:38.94 Sea 5.4m deep (22.7psia)
@13:44:39.07 SamplePump.in :sample
@13:44:39.14 SamplePump.seek 1000ml,1:23:53
@13:44:41.80 <sampling> Cartridge 35 filtering 1/1000ml at 0.29ml/s with 26.9psi vacuum 2.5m deep (18.4psia)
@13:45:41.80 Cartridge 35 filtering 20/1000ml at 0.29ml/s with 21.2psi vacuum 3.3m deep (19.5psia)
@13:46:41.81 Cartridge 35 filtering 38/1000ml at 0.29ml/s with 21.4psi vacuum 3.7m deep (20.2psia)
@13:47:41.80 Cartridge 35 filtering 56/1000ml at 0.31ml/s with 25.9psi vacuum 4.1m deep (20.8psia)
@13:48:41.81 Cartridge 35 filtering 73/1000ml at 0.27ml/s with 29.1psi vacuum
@13:49:42.22 Cartridge 35 filtering 90/1000ml at 0.29ml/s with 22.6psi vacuum 4.2m deep (20.9psia)
@13:50:41.81 Cartridge 35 filtering 106/1000ml at 0.31ml/s with 23.7psi vacuum 5.6m deep (23.0psia)
@13:51:41.80 Cartridge 35 filtering 123/1000ml at 0.30ml/s with 25.8psi vacuum 2.9m deep (18.9psia)
@13:52:41.81 Cartridge 35 filtering 138/1000ml at 0.29ml/s with 23.4psi vacuum 4.1m deep (20.8psia)
@13:53:41.80 Cartridge 35 filtering 154/1000ml at 0.27ml/s with 21.7psi vacuum 4.2m deep (20.9psia)
@13:54:41.81 Cartridge 35 filtering 169/1000ml at 0.25ml/s with 20.5psi vacuum 3.9m deep (20.4psia)
@13:55:41.80 Cartridge 35 filtering 184/1000ml at 0.20ml/s with 23.3psi vacuum 4.0m deep (20.6psia)
@13:56:41.81 Cartridge 35 filtering 198/1000ml at 0.22ml/s with 20.6psi vacuum 2.1m deep (17.9psia)
@13:57:41.80 Cartridge 35 filtering 213/1000ml at 0.25ml/s with 25.1psi vacuum 4.5m deep (21.4psia)
@13:58:41.81 Cartridge 35 filtering 227/1000ml at 0.20ml/s with 23.8psi vacuum 2.2m deep (17.9psia)
@13:59:41.80 Cartridge 35 filtering 241/1000ml at 0.23ml/s with 26.4psi vacuum 3.5m deep (19.9psia)
@14:00:41.81 Cartridge 35 filtering 255/1000ml at 0.20ml/s with 24.0psi vacuum 2.9m deep (19.0psia)
@14:01:41.80 Cartridge 35 filtering 268/1000ml at 0.25ml/s with 22.6psi vacuum 4.3m deep (21.1psia)
@14:02:41.81 Cartridge 35 filtering 281/1000ml at 0.24ml/s with 23.3psi vacuum 2.2m deep (17.9psia)
@14:03:41.80 Cartridge 35 filtering 295/1000ml at 0.20ml/s with 27.8psi vacuum 3.3m deep (19.5psia)
@14:04:41.81 Cartridge 35 filtering 308/1000ml at 0.24ml/s with 24.3psi vacuum 3.6m deep (20.0psia)
@14:05:41.80 Cartridge 35 filtering 321/1000ml at 0.20ml/s with 26.0psi vacuum 4.0m deep (20.6psia)
@14:06:41.81 Cartridge 35 filtering 333/1000ml at 0.23ml/s with 24.8psi vacuum 3.5m deep (19.9psia)
@14:07:41.80 Cartridge 35 filtering 346/1000ml at 0.20ml/s with 27.8psi vacuum 2.5m deep (18.4psia)
@14:08:41.81 Cartridge 35 filtering 358/1000ml at 0.20ml/s with 28.2psi vacuum 3.2m deep (19.4psia)
@14:09:41.80 Cartridge 35 filtering 371/1000ml at 0.20ml/s with 24.1psi vacuum 2.7m deep (18.7psia)
@14:10:41.81 Cartridge 35 filtering 383/1000ml at 0.20ml/s with 29.8psi vacuum
@14:11:41.80 Cartridge 35 filtering 395/1000ml at 0.20ml/s with 27.9psi vacuum 3.3m deep (19.6psia)
@14:12:16.22 <FILTERING> Flow averaged 0.24ml/s over 27:38
@14:12:16.25 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi
@14:12:16.82 Relieved excess vacuum in .6 seconds
@14:12:16.85 SamplePump.seek 1000ml,50:22
@14:12:41.80 <sampling> Cartridge 35 filtering 407/1000ml at 0.21ml/s with 24.4psi vacuum 2.8m deep (18.8psia)
@14:12:57.43 <FILTERING> Flow averaged 0.20ml/s over 41 seconds
@14:12:57.46 Waiting up to 14 seconds for vacuum to reduce from 28.3 to 18.0psi
@14:12:58.03 Relieved excess vacuum in .6 seconds
@14:12:58.06 SamplePump.seek 1000ml,49:41
@14:13:16.37 Flow averaged 0.20ml/s over 19 seconds
@14:13:16.40 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi
@14:13:16.97 Relieved excess vacuum in .6 seconds
@14:13:17.00 SamplePump.seek 1000ml,49:22
@14:13:21.69 Flow estimate reduced to 0.16ml/s
@14:13:21.72 <closeIntake> Intake.close
@14:13:21.77 <closeExhaust> Exhaust.close
@14:13:21.96 <FILTERING> Toroid.in :hold
@14:13:22.02 Toroid.seek :bypass
@14:13:23.65 Toroid.in :default
@14:13:23.71 Toroid.seek :clear
@14:13:25.33 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@14:13:25.34 Pressure.coast
@14:13:25.53 Sampled  414.8ml
@14:13:27.54 <LRAUV> -> Cmd.startProcessing
@14:13:28.98 <PROCESSING> SPR.command \"read_sequences\"
@14:13:29.45 SPR.command \"stats_on\"
@14:13:29.49 SPR.command \"start 0 1\"
@14:13:31.50 <SPRlogger> Begun log /var/log/esp/spr21apr2214h.spr
@14:13:32.52 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3)
@14:13:34.20 <PROCESSING> Handoff.select :waste
@14:13:40.88 Syringe tare is 20% of full scale
@14:13:40.90 PinPort.in :locking
@14:13:40.97 PinPort.seek :locked
@14:13:41.45 <posArchive> Archive.in :fast
@14:13:41.51 <posLyS2> Lysis2.in :fast
@14:13:41.56 <PROCESSING> Lysis1.seek 1350uL
@14:13:41.68 <posArchive> Archive.seek :full
@14:13:41.73 <posLyS2> Lysis2.seek :full
@14:13:43.81 <PROCESSING> Plunge.seek :reagent
@14:13:44.71 Evacuating puck with filter bubble point of 50psi
@14:13:44.73 PinPort.seek :locked
@14:13:44.81 Handoff.select :waste
@14:13:51.39 Air syringe pumping up to 1 stroke to reach 8psi
@14:13:51.40 Air stroke #1 of 1
@14:13:51.48 Air.in :pressurize
@14:13:51.54 Air.seek :pressurized
@14:14:02.81 0.4psi after pressurize
@14:14:02.82 Air.in :compress
@14:14:02.89 Air.seek :evac
@14:14:06.98 0.6psi after compress
@14:14:19.56 0.2psi after compress (stable)
@14:14:19.57 Air.in :default
@14:14:19.63 Air.hold
@14:14:19.80 at 0.1psi Opened to waste
@14:14:19.81 Handoff.select :blocked
@14:14:19.94 Archive syringe pumping up to 5 strokes to reach 50psi
@14:14:19.95 Archive stroke #1 of 5
@14:14:20.05 Archive.in :pressurize
@14:14:20.12 Archive.seek :pressurized
@14:14:32.87 10.5psi after pressurize
@14:14:32.88 Archive.in :compress
@14:14:32.96 Archive.seek :empty
@14:14:48.85 32.5psi after compress
@14:15:03.93 36.5psi after compress (stable)
@14:15:03.95 Archive.in :gulp
@14:15:04.03 Archive.seek :topVent
@14:15:11.23 36.7psi after gulp
@14:15:23.80 36.6psi after gulp (stable)
@14:15:23.81 Archive stroke #2 of 5
@14:15:23.90 Archive.in :pressurize
@14:15:23.96 Archive.seek :pressurized
@14:15:36.72 36.4psi after pressurize
@14:15:36.74 Archive.in :compress
@14:15:36.82 Archive.seek :empty
@14:15:50.10 Reached 50.3 of 50.0psi after pumping 3404uL (1.97 strokes)
@14:15:50.11 Archive.in :default
@14:15:50.18 Archive.hold
@14:15:50.24 Handoff.select :waste
@14:16:00.43 delay 1:00
@14:17:00.46 Handoff.select :blocked
@14:17:23.15 Handoff.select :waste
@14:17:23.27 0.1psi at evacEnd
@14:17:23.28 Handoff.select :blocked
@14:17:23.41 Archive.in :fast
@14:17:23.48 Archive.seek :topVent
@14:17:36.99 Stable @0.5psi after Handoff blocked
@14:17:37.02 Archive syringe pumping up to 5 strokes to reach 30psi
@14:17:37.03 Archive stroke #1 of 5
@14:17:37.52 Archive.in :pressurize
@14:17:37.59 Archive.seek :pressurized
@14:17:50.34 0.7psi after pressurize
@14:17:50.36 Archive.in :compress
@14:17:50.43 Archive.seek :empty
@14:18:06.33 2.3psi after compress
@14:18:46.40 23.2psi after compress (stable)
@14:18:46.42 Archive.in :gulp
@14:18:46.49 Archive.seek :topVent
@14:18:53.70 24.5psi after gulp
@14:19:06.27 25.3psi after gulp (stable)
@14:19:06.29 Archive stroke #2 of 5
@14:19:06.38 Archive.in :pressurize
@14:19:06.44 Archive.seek :pressurized
@14:19:19.18 26.2psi after pressurize
@14:19:19.19 Archive.in :compress
@14:19:19.27 Archive.seek :empty
@14:19:35.16 27.7psi after compress
@14:19:42.71 Reached 30.5 of 30.5psi after pumping 3460uL (2.00 strokes)
@14:19:42.73 Archive.in :default
@14:19:42.79 Archive.hold
@14:19:42.86 Handoff.select :waste
@14:19:58.47 delay 1:00
@14:20:58.49 Handoff.select :blocked
@14:21:21.18 Handoff.select :waste
@14:21:21.30 2.3psi at evacEnd
@14:21:21.32 Air.seek :fullVent
@14:21:22.77 Archive.in :fast
@14:21:22.84 Archive.seek :topVent
@14:21:30.00 Seawater evacuated, Presure above/below filter released. Lysis begins
@14:21:30.09 Lysis1.in :slow
@14:21:30.15 Lysis1.seek 1050uL
@14:22:00.18 delay 5 seconds
@14:22:05.20 Handoff.select :blocked
@14:22:05.37 Lysis1.seek 450uL
@14:23:05.40 delay 3 seconds
@14:23:08.47 Air.in to mix into 1.37806 parts Lysis1
@14:23:08.98 <Airmixer> Air.seek :evac
@14:23:09.02 <PROCESSING> Lysis1.seek 300uL
@14:23:24.15 Lysis1.seek :empty
@14:23:54.29 Lysis buffer delivered @12.4psi
@14:23:54.30 Polling Can every 2:00
@14:23:54.85 PuckHeater.configure :default #ambient 11.7C
@14:23:54.90 PuckHeater.seek 80.2C
@14:25:01.29 PuckHeater.hold 2:00,80.2C
@14:27:01.32 Polling Can every 2:00
@14:27:01.88 PuckHeater.configure :default #ambient 11.8C
@14:27:01.93 PuckHeater.hold 30 seconds,79.2C
@14:27:32.08 Polling Can every 2:00
@14:27:32.63 PuckHeater.configure :default #ambient 11.8C
@14:27:32.70 PuckHeater.hold 30 seconds,78.6C
@14:28:02.87 Polling Can every 2:00
@14:28:03.42 PuckHeater.configure :default #ambient 11.8C
@14:28:03.47 PuckHeater.hold 10:00,78.1C
@14:38:02.90 Lysis complete. Syringe pressure @14.9psi
@14:38:02.92 Handoff.select :blocked
@14:38:02.97 Archive syringe pumping up to 3 strokes to reach 40psi
@14:38:02.98 Archive stroke #1 of 3
@14:38:03.07 Archive.in :pressurize
@14:38:03.13 Archive.seek :pressurized
@14:38:15.89 14.7psi after pressurize
@14:38:15.91 Archive.in :compress
@14:38:15.98 Archive.seek :empty
@14:38:26.38 Reached 40.5 of 40.0psi after pumping 1613uL (0.93 strokes)
@14:38:26.39 Archive.in :default
@14:38:26.46 Archive.hold
@14:38:26.54 delay 2 seconds
@14:38:28.56 Archive.in :fast
@14:38:28.63 Archive.seek :full
@14:38:35.42 Lysis1.seek 138uL
@14:38:49.31 delay 1 second
@14:38:50.34 <fillArchive> Archive.seek :full
@14:38:50.36 <PROCESSING> Lysis1.seek :empty
@14:39:04.35 Initial air pressure @45.5psi
@14:39:04.43 Lysis1.in :extract
@14:39:04.50 Lysis1.seek 600uL,nil
@14:39:07.44 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@14:39:07.45 Archive stroke #1 of 6
@14:39:07.53 Archive.in :pressurize
@14:39:07.60 Archive.seek :pressurized
@14:39:20.34 15.4psi after pressurize
@14:39:20.35 Archive.in :compress
@14:39:20.44 Archive.seek :empty
@14:39:35.96 Archive.in :default
@14:39:36.01 Archive.hold
@14:39:36.28 <PROCESSING> Initial 600uL lysate @19.0psi
@14:39:36.29 Handoff.select :blocked
@14:39:36.45 Initial air pressure @20.6psi
@14:39:36.53 Lysis1.seek 825uL,nil
@14:39:37.81 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@14:39:37.82 Archive stroke #1 of 6
@14:39:37.90 Archive.in :compress
@14:39:37.97 Archive.seek :empty
@14:39:38.37 14.8psi after compress
@14:39:42.62 Reached 17.0 of 17.0psi after pumping 1730uL (1.00 strokes)
@14:39:42.64 Archive.in :default
@14:39:42.70 Archive.hold
@14:39:43.46 Archive syringe pumping up to 6 strokes to reach 17psi
@14:39:43.47 Archive stroke #1 of 6
@14:39:43.60 Archive.in :compress
@14:39:43.66 Archive.seek :empty
@14:39:43.85 15.5psi after compress
@14:39:56.43 15.8psi after compress (stable)
@14:39:58.39 Archive.in :default
@14:39:58.44 Archive.hold
@14:39:58.61 <PROCESSING> Recovered 825uL lysate @15.6psi
@14:39:58.63 Air.in :halfSpeed
@14:39:58.70 Air.seek :fullVent
@14:40:00.95 Puck Top Vented, bottom @12.6psi
@14:40:00.96 Handoff.select :waste
@14:40:01.08 delay 5 seconds
@14:40:06.14 Handoff open to waste, bottom @0.2psi
@14:40:06.16 Air.in :default
@14:40:06.23 Air.seek :evac
@14:40:07.67 Lysis2.in :DAmix
@14:40:07.78 Lysis1.in :DAmix
@14:40:08.21 <Lysis2mixer> Lysis2.seek 1480uL
@14:40:08.24 <PROCESSING> Lysis1.seek 575uL
@14:40:43.33 delay 5 seconds
@14:40:48.35 Handoff.select SPR
@14:40:48.46 SPR.command \"start 2 14\"
@14:40:49.64 delay 10 seconds
@14:40:59.66 SPR.command \"start 1 0\"
@14:41:03.39 Waiting up to 4:00 for SPR to enter state 1
@14:43:06.22 SPR.command \"hold_on\"
@14:43:07.63 SPR Dilution 1 of 1 -- Running Experiment 1
@14:43:08.06 <Lysis2mixer> Lysis2.seek 1180uL
@14:43:08.12 <PROCESSING> Lysis1.seek 275uL
@14:43:50.15 volL1 = 300.0 and volL2 = 300.0
@14:43:50.16 delay 15 seconds
@14:44:05.18 SPR.command \"hold_off\"
@14:44:08.28 Waiting up to 38:00 for SPR to enter state 7
@15:10:51.82 SPR Analysis has Completed
@15:10:51.84 Handoff.select :waste
@15:10:52.76 <SPRlogger> Ended log /var/log/esp/spr21apr2214h.spr
@15:10:52.77 <PROCESSING> SPR.command \"stats_off\"
@15:10:54.24 <LRAUV> -> Cmd.stop
@15:10:54.34 <closeHandoff> Handoff.select :close
@15:10:54.47 <LRAUV> Gate.power :heat,:OFF
@15:10:54.49 Gate.power :cartridge,:OFF
@15:10:54.57 Carousel.seek 35
@15:10:54.75 Plunge.seek :locked
@15:10:55.63 PinPort.in :unlocking
@15:10:55.69 PinPort.seek :clear
@15:10:56.23 <Archive> Archive.in :unlocking
@15:10:56.35 Archive.seek :clear
@15:10:56.43 <Air> Air.in :unlocking
@15:10:56.56 Air.seek :clear
@15:10:56.64 <Lysis1> Lysis1.in :unlocking
@15:10:56.77 Lysis1.seek :clear
@15:10:56.83 <Lysis2> Lysis2.in :unlocking
@15:10:56.91 Lysis2.seek :clear
@15:11:00.42 <LRAUV> Archive.coast
@15:11:00.47 Air.coast
@15:11:00.52 Lysis1.coast
@15:11:00.57 Lysis2.coast
@15:11:00.62 Plunge.coast
@15:11:00.67 SamplePump.coast
@15:11:00.72 Toroid.coast
@15:11:00.77 Carousel.coast
@15:11:00.82 PinPort.coast
@15:11:01.01 Gate.power :motor,:OFF
@15:11:01.03 Gate.power :core,:OFF
@15:11:01.05 Gate.power :analytic1,:OFF
@15:11:01.07 Gate.power :analytic2,:OFF
@15:11:01.09 Gate.power :analytic3,:OFF
@15:11:01.11 Gate.power :main,:OFF
@15:11:01.13 Safely stopped and ready to power off
##-- file closed 2021-04-23T00:18:42.294Z

##-- file opened 2021-04-23T00:18:42.295Z
@17:18:42.51 -> showStatus
@17:18:44.53 -> Cmd.loadCartridge -6
@17:18:44.67 Gate.power :main,:ON,limit to 5000mA
@17:18:44.70 Gate.power :analytic1,:ON,limit to 5000mA
@17:18:44.72 Gate.power :analytic2,:ON,limit to 5000mA
@17:18:44.75 Gate.power :analytic3,:ON,limit to 5000mA
@17:18:44.88 Powering core bus
@17:18:47.16 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@17:18:47.30 <LRAUV> Archive.configure :default
@17:18:47.42 Air.configure :default
@17:18:47.52 Lysis1.configure :default
@17:18:47.63 Lysis2.configure :default
@17:18:47.75 Plunge.configure :default
@17:18:47.87 SamplePump.configure :default
@17:18:48.01 ExtraPump.configure :default
@17:18:48.12 Toroid.configure :default
@17:18:48.25 Carousel.configure :default
@17:18:48.38 Pressure.configure :default
@17:18:48.49 PinPort.configure :default
@17:18:48.56 All elf controllers running firmware version 4.741
@17:18:48.59 Gate.power :motor,:ON,limit to 5000mA
@17:18:48.74 <Archive_home> Archive.in :default
@17:18:49.05 Archive.home.jog 0
@17:18:49.22 <Toroid_home> Toroid.in :default
@17:18:49.30 <Plunge_home> Plunge.in :default
@17:18:49.33 <Lysis1_home> Lysis1.in :default
@17:18:49.39 <PinPort_home> PinPort.in :default
@17:18:49.44 <Lysis2_home> Lysis2.in :default
@17:18:49.47 <Air_home> Air.in :default
@17:18:49.66 <Plunge_home> Plunge.home.jog 0
@17:18:49.70 <Toroid_home> Toroid.home.jog 0
@17:18:50.06 <Lysis1_home> Lysis1.home.jog 0
@17:18:50.12 <PinPort_home> PinPort.home.jog 0
@17:18:50.14 <Lysis2_home> Lysis2.home.jog 0
@17:18:50.18 <Air_home> Air.home.jog 0
@17:18:50.52 <Archive_home> Archive.in :homing
@17:18:50.73 <Toroid_home> Toroid.in :homing
@17:18:50.76 <Plunge_home> Plunge.in :homing
@17:18:50.81 <Archive_home> Archive.jog 151
@17:18:50.87 <Lysis1_home> Lysis1.in :homing
@17:18:50.89 <PinPort_home> PinPort.in :homing
@17:18:51.04 <Toroid_home> Toroid.jog 606
@17:18:51.12 <Plunge_home> Plunge.jog 1575
@17:18:51.15 <PinPort_home> PinPort.jog -107
@17:18:51.28 <Lysis1_home> Lysis1.jog 1417
@17:18:51.42 <Air_home> Air.in :homing
@17:18:51.45 <Lysis2_home> Lysis2.in :homing
@17:18:51.52 <Air_home> Air.jog 167
@17:18:51.55 <Lysis2_home> Lysis2.jog 150
@17:18:51.67 <Archive_home> Archive.setRawPosition! 50
@17:18:51.69 <PinPort_home> PinPort.setRawPosition! 0
@17:18:51.77 PinPort.seek :home
@17:18:51.79 <Archive_home> Archive.seek :home
@17:18:51.93 <PinPort_home> PinPort.in :default
@17:18:52.02 <Air_home> Air.setRawPosition! 50
@17:18:52.08 Air.seek :home
@17:18:52.17 <Lysis2_home> Lysis2.setRawPosition! 50
@17:18:52.19 <Archive_home> Archive.in :default
@17:18:52.26 <Lysis2_home> Lysis2.seek :home
@17:18:52.37 <Air_home> Air.in :default
@17:18:52.48 <Plunge_home> Plunge.setRawPosition! 50
@17:18:52.50 <Lysis1_home> Lysis1.setRawPosition! 50
@17:18:52.58 <Plunge_home> Plunge.seek :home
@17:18:52.62 <Lysis1_home> Lysis1.seek :home
@17:18:52.75 <Lysis2_home> Lysis2.in :default
@17:18:52.93 <Plunge_home> Plunge.in :default
@17:18:53.01 <Lysis1_home> Lysis1.in :default
@17:18:53.25 <Toroid_home> Toroid.setRawPosition! 0
@17:18:53.30 Toroid.seek :home
@17:18:53.39 Toroid.in :default
@17:18:53.52 <LRAUV> Toroid.seek :clear
@17:18:55.15 Carousel.in :default
@17:18:55.21 Carousel.home.jog 0
@17:18:55.70 Carousel.in :default
@17:18:55.76 Carousel.seek 35
@17:18:55.92 Selecting Cartridge 36
@17:18:56.16 Carousel.seek 36
@17:18:56.64 Lysis1.in :fast
@17:18:56.71 Lysis1.seek :locked
@17:18:59.30 <Plunge> Plunge.seek :locked
@17:18:59.42 <Air> Air.in :fast
@17:18:59.48 Air.seek :locked
@17:19:02.08 <LRAUV> Powering cartridge bus
@17:19:04.20 Gate.setIR 1750,5000,0,6
@17:19:04.33 Gate.setIR 0,5000,0,6
@17:19:04.38 Gate.power :cartridge,:OFF
@17:19:04.92 Mounted archive Cartridge
@17:19:24.13 -> Cmd.startFiltering
@17:19:24.58 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@17:19:24.59 SamplePump.setPosition! 0ml
@17:19:24.64 Bypass.open
@17:19:26.12 Intake.open
@17:19:26.94 Sea 6.7m deep (24.7psia)
@17:19:26.95 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@17:19:29.53 Vacuum tare is 51% of full scale
@17:19:29.65 Pressure.in :default
@17:19:29.71 Pressure.hold
@17:19:29.88 SamplePump.in :prime
@17:19:29.94 SamplePump.seek 10ml,1:21
@17:19:39.32 Pressure.coast
@17:19:39.37 SamplePump.setPosition! 0ml
@17:19:39.42 Exhaust.open
@17:19:40.83 <closeBypass> Bypass.close
@17:19:42.39 <FILTERING> Pressure.in :default
@17:19:42.45 Pressure.hold
@17:19:42.56 Sea 5.7m deep (23.2psia)
@17:19:42.69 SamplePump.in :prime
@17:19:42.75 SamplePump.seek 100ml,8:51
@17:21:52.59 Pressure.coast
@17:21:52.64 Bypass.open
@17:21:53.38 Intake.close
@17:21:54.22 Toroid.in :hold
@17:21:54.28 Toroid.seek :crack
@17:21:55.89 Toroid.seek :cartridge
@17:21:57.00 Toroid.in :default
@17:21:57.06 Toroid.seek :clear
@17:21:59.02 Pressurizing cartridge to 26psi above 24.4psia
@17:21:59.03 SamplePump.setPosition! 0ml
@17:21:59.07 Intake.open
@17:21:59.79 Bypass.close
@17:22:00.56 Exhaust.close
@17:22:01.36 Pressure.in :default
@17:22:01.42 Pressure.hold
@17:22:01.59 SamplePump.in :charge
@17:22:01.66 SamplePump.seek 2.3ml,42 seconds
@17:22:07.03 Pressure.coast
@17:22:07.08 Intake.close
@17:22:07.86 Pressurized cartridge with 0.126ml to 26.3psi
@17:22:07.87 Isolated @43.9psia (19.6psi)
@17:22:07.89 Verifying cartridge holds at least 0.75..0.85 of 43.9psia
@17:22:07.92 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@17:22:12.55 Sampling 1000.0ml
@17:22:12.56 SamplePump.setPosition! 0ml
@17:22:12.62 Exhaust.open
@17:22:13.32 Intake.open
@17:22:14.16 Pressure.in :default
@17:22:14.22 Pressure.hold
@17:22:14.33 Sea 5.5m deep (22.9psia)
@17:22:14.46 SamplePump.in :sample
@17:22:14.54 SamplePump.seek 1000ml,1:23:53
@17:22:17.19 <sampling> Cartridge 36 filtering 1/1000ml at 0.35ml/s with 25.3psi vacuum 3.7m deep (20.1psia)
@17:23:17.20 Cartridge 36 filtering 19/1000ml at 0.32ml/s with 21.5psi vacuum 4.2m deep (20.8psia)
@17:24:17.20 Cartridge 36 filtering 38/1000ml at 0.29ml/s with 21.5psi vacuum 3.5m deep (19.9psia)
@17:25:17.20 Cartridge 36 filtering 55/1000ml at 0.31ml/s with 25.8psi vacuum 3.9m deep (20.4psia)
@17:26:17.20 Cartridge 36 filtering 73/1000ml at 0.24ml/s with 24.9psi vacuum 3.1m deep (19.3psia)
@17:27:17.20 Cartridge 36 filtering 90/1000ml at 0.29ml/s with 27.2psi vacuum 3.1m deep (19.3psia)
@17:28:17.61 Cartridge 36 filtering 107/1000ml at 0.23ml/s with 23.5psi vacuum 2.4m deep (18.3psia)
@17:29:17.20 Cartridge 36 filtering 123/1000ml at 0.27ml/s with 21.5psi vacuum 5.1m deep (22.2psia)
@17:30:17.20 Cartridge 36 filtering 139/1000ml at 0.22ml/s with 25.8psi vacuum
@17:31:17.20 Cartridge 36 filtering 154/1000ml at 0.29ml/s with 21.0psi vacuum 5.8m deep (23.2psia)
@17:32:17.20 Cartridge 36 filtering 170/1000ml at 0.27ml/s with 22.9psi vacuum 4.2m deep (20.9psia)
@17:33:17.20 Cartridge 36 filtering 185/1000ml at 0.26ml/s with 22.1psi vacuum 3.5m deep (19.8psia)
@17:34:17.20 Cartridge 36 filtering 200/1000ml at 0.22ml/s with 21.2psi vacuum 4.0m deep (20.6psia)
@17:35:17.20 Cartridge 36 filtering 215/1000ml at 0.25ml/s with 22.9psi vacuum 1.7m deep (17.3psia)
@17:36:17.20 Cartridge 36 filtering 229/1000ml at 0.27ml/s with 22.5psi vacuum 5.5m deep (22.9psia)
@17:37:17.20 Cartridge 36 filtering 243/1000ml at 0.24ml/s with 22.9psi vacuum 2.8m deep (18.9psia)
@17:38:17.20 Cartridge 36 filtering 257/1000ml at 0.24ml/s with 24.0psi vacuum 2.7m deep (18.7psia)
@17:39:17.20 Cartridge 36 filtering 271/1000ml at 0.26ml/s with 24.5psi vacuum 5.0m deep (22.0psia)
@17:40:17.20 Cartridge 36 filtering 284/1000ml at 0.23ml/s with 23.6psi vacuum 2.8m deep (18.8psia)
@17:41:17.20 Cartridge 36 filtering 298/1000ml at 0.23ml/s with 21.0psi vacuum 4.9m deep (21.9psia)
@17:42:17.20 Cartridge 36 filtering 311/1000ml at 0.20ml/s with 24.0psi vacuum 2.6m deep (18.5psia)
@17:43:17.25 Cartridge 36 filtering 324/1000ml at 0.20ml/s with 24.8psi vacuum 3.4m deep (19.7psia)
@17:44:17.20 Cartridge 36 filtering 337/1000ml at 0.21ml/s with 20.9psi vacuum 4.3m deep (21.1psia)
@17:45:17.20 Cartridge 36 filtering 350/1000ml at 0.24ml/s with 22.4psi vacuum 2.8m deep (18.9psia)
@17:46:17.20 Cartridge 36 filtering 363/1000ml at 0.20ml/s with 25.2psi vacuum 3.6m deep (20.1psia)
@17:47:17.20 Cartridge 36 filtering 375/1000ml at 0.21ml/s with 20.6psi vacuum 3.0m deep (19.2psia)
@17:48:17.20 Cartridge 36 filtering 388/1000ml at 0.20ml/s with 24.4psi vacuum
@17:49:17.20 Cartridge 36 filtering 400/1000ml at 0.20ml/s with 23.0psi vacuum 6.4m deep (24.1psia)
@17:50:17.20 Cartridge 36 filtering 412/1000ml at 0.22ml/s with 24.8psi vacuum 2.4m deep (18.3psia)
@17:51:17.20 Cartridge 36 filtering 424/1000ml at 0.20ml/s with 29.5psi vacuum 4.5m deep (21.4psia)
@17:51:50.22 <FILTERING> Flow averaged 0.24ml/s over 29:36
@17:51:50.25 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@17:51:50.82 Relieved excess vacuum in .6 seconds
@17:51:50.85 SamplePump.seek 1000ml,47:56
@17:52:17.19 <sampling> Cartridge 36 filtering 437/1000ml at 0.21ml/s with 22.5psi vacuum 3.5m deep (19.9psia)
@17:52:34.64 <FILTERING> Flow averaged 0.20ml/s over 44 seconds
@17:52:34.67 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi
@17:52:35.24 Relieved excess vacuum in .6 seconds
@17:52:35.27 SamplePump.seek 1000ml,47:12
@17:52:56.21 Flow averaged 0.20ml/s over 21 seconds
@17:52:56.24 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi
@17:52:56.80 Relieved excess vacuum in .6 seconds
@17:52:56.83 SamplePump.seek 1000ml,46:51
@17:53:17.20 <sampling> Cartridge 36 filtering 448/1000ml at 0.21ml/s with 23.6psi vacuum 2.8m deep (18.8psia)
@17:53:23.93 <FILTERING> Flow averaged 0.20ml/s over 28 seconds
@17:53:23.96 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi
@17:53:24.53 Relieved excess vacuum in .6 seconds
@17:53:24.56 SamplePump.seek 1000ml,46:23
@17:53:38.57 Flow averaged 0.20ml/s over 15 seconds
@17:53:38.59 <closeIntake> Intake.close
@17:53:38.63 <closeExhaust> Exhaust.close
@17:53:38.82 <FILTERING> Toroid.in :hold
@17:53:38.89 Toroid.seek :bypass
@17:53:40.52 Toroid.in :default
@17:53:40.58 Toroid.seek :clear
@17:53:42.19 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@17:53:42.20 Pressure.coast
@17:53:42.39 Sampled  452.5ml
@17:53:44.62 <LRAUV> -> Cmd.startProcessing
@17:53:44.99 <PROCESSING> Plunge.seek :reagent
@17:53:45.82 Sample loop closed. Ready to Deliver Reagents
@17:53:45.83 Archive.seek :sealed
@17:53:50.11 Archive.seek :evac
@17:54:04.05 delay 1:00
@17:55:04.07 Archive.seek :clear
@17:55:21.81 RNAlater soak for 10:00
@18:05:21.83 Archive.seek :tVent
@18:05:24.41 delay 1 second
@18:05:25.43 Archive.seek :evac
@18:05:41.06 delay 10 seconds
@18:05:51.09 Sample preserved and evacuated
@18:05:52.25 <LRAUV> -> Cmd.stop
@18:05:52.39 Carousel.seek 36
@18:05:52.56 Plunge.seek :locked
@18:05:53.51 <Archive> Archive.in :unlocking
@18:05:53.63 Archive.seek :clear
@18:05:53.71 <Air> Air.in :unlocking
@18:05:53.82 Air.seek :clear
@18:05:53.90 <Lysis1> Lysis1.in :unlocking
@18:05:54.01 Lysis1.seek :clear
@18:05:57.67 <LRAUV> Archive.coast
@18:05:57.72 Air.coast
@18:05:57.77 Lysis1.coast
@18:05:57.82 Lysis2.coast
@18:05:57.87 Plunge.coast
@18:05:57.92 SamplePump.coast
@18:05:57.97 Toroid.coast
@18:05:58.02 Carousel.coast
@18:05:58.08 PinPort.coast
@18:05:58.27 Gate.power :motor,:OFF
@18:05:58.29 Gate.power :core,:OFF
@18:05:58.31 Gate.power :analytic1,:OFF
@18:05:58.33 Gate.power :analytic2,:OFF
@18:05:58.35 Gate.power :analytic3,:OFF
@18:05:58.37 Gate.power :main,:OFF
@18:05:58.39 Safely stopped and ready to power off
##-- file closed 2021-04-23T01:17:53.506Z

##-- file opened 2021-04-23T01:17:53.507Z
@18:17:53.64 -> showStatus
@18:17:55.66 -> Cmd.loadCartridge -9
@18:17:55.81 Gate.power :main,:ON,limit to 5000mA
@18:17:55.82 Gate.power :analytic1,:ON,limit to 5000mA
@18:17:55.84 Gate.power :analytic2,:ON,limit to 5000mA
@18:17:55.86 Gate.power :analytic3,:ON,limit to 5000mA
@18:17:56.00 Powering core bus
@18:17:58.28 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@18:17:58.41 <LRAUV> Archive.configure :default
@18:17:58.53 Air.configure :default
@18:17:58.63 Lysis1.configure :default
@18:17:58.73 Lysis2.configure :default
@18:17:58.85 Plunge.configure :default
@18:17:58.98 SamplePump.configure :default
@18:17:59.13 ExtraPump.configure :default
@18:17:59.23 Toroid.configure :default
@18:17:59.36 Carousel.configure :default
@18:17:59.48 Pressure.configure :default
@18:17:59.58 PinPort.configure :default
@18:17:59.65 All elf controllers running firmware version 4.741
@18:17:59.68 Gate.power :motor,:ON,limit to 5000mA
@18:17:59.84 <Archive_home> Archive.in :default
@18:18:00.15 Archive.home.jog 0
@18:18:00.31 <Toroid_home> Toroid.in :default
@18:18:00.38 <Plunge_home> Plunge.in :default
@18:18:00.42 <Lysis1_home> Lysis1.in :default
@18:18:00.46 <Air_home> Air.in :default
@18:18:00.50 <PinPort_home> PinPort.in :default
@18:18:00.60 <Lysis2_home> Lysis2.in :default
@18:18:01.09 <Toroid_home> Toroid.home.jog 0
@18:18:01.14 <Plunge_home> Plunge.home.jog 0
@18:18:01.17 <Lysis1_home> Lysis1.home.jog 0
@18:18:01.20 <PinPort_home> PinPort.home.jog 0
@18:18:01.22 <Air_home> Air.home.jog 0
@18:18:01.26 <Lysis2_home> Lysis2.home.jog 0
@18:18:01.72 <Archive_home> Archive.in :homing
@18:18:01.93 <Plunge_home> Plunge.in :homing
@18:18:01.96 <Lysis2_home> Lysis2.in :homing
@18:18:01.99 <Air_home> Air.in :homing
@18:18:02.02 <Toroid_home> Toroid.in :homing
@18:18:02.06 <Lysis1_home> Lysis1.in :homing
@18:18:02.10 <PinPort_home> PinPort.in :homing
@18:18:02.15 <Archive_home> Archive.jog 1415
@18:18:02.33 <Lysis2_home> Lysis2.jog 1430
@18:18:02.39 <Plunge_home> Plunge.jog 1586
@18:18:02.42 <PinPort_home> PinPort.jog -106
@18:18:02.45 <Lysis1_home> Lysis1.jog 1417
@18:18:02.48 <Air_home> Air.jog 156
@18:18:02.51 <Toroid_home> Toroid.jog 602
@18:18:02.83 <PinPort_home> PinPort.setRawPosition! 0
@18:18:02.87 PinPort.seek :home
@18:18:02.96 PinPort.in :default
@18:18:03.06 <Air_home> Air.setRawPosition! 50
@18:18:03.11 Air.seek :home
@18:18:03.39 Air.in :default
@18:18:03.47 <Archive_home> Archive.setRawPosition! 50
@18:18:03.52 Archive.seek :home
@18:18:03.68 <Lysis2_home> Lysis2.setRawPosition! 50
@18:18:03.73 Lysis2.seek :home
@18:18:03.85 <Plunge_home> Plunge.setRawPosition! 50
@18:18:03.88 <Archive_home> Archive.in :default
@18:18:03.91 <Lysis1_home> Lysis1.setRawPosition! 50
@18:18:03.96 <Plunge_home> Plunge.seek :home
@18:18:04.08 <Lysis1_home> Lysis1.seek :home
@18:18:04.19 <Lysis2_home> Lysis2.in :default
@18:18:04.32 <Plunge_home> Plunge.in :default
@18:18:04.40 <Lysis1_home> Lysis1.in :default
@18:18:04.68 <Toroid_home> Toroid.setRawPosition! 0
@18:18:04.72 Toroid.seek :home
@18:18:04.82 Toroid.in :default
@18:18:04.94 <LRAUV> Toroid.seek :clear
@18:18:06.55 Carousel.in :default
@18:18:06.61 Carousel.home.jog 0
@18:18:07.10 Carousel.in :default
@18:18:07.16 Carousel.seek 36
@18:18:07.33 Selecting Cartridge 33
@18:18:07.57 Carousel.seek 33
@18:18:08.46 Lysis1.in :fast
@18:18:08.52 Lysis1.seek :locked
@18:18:11.12 <Plunge> Plunge.seek :locked
@18:18:11.24 <Air> Air.in :fast
@18:18:11.31 Air.seek :locked
@18:18:13.90 <LRAUV> Powering cartridge bus
@18:18:16.02 Gate.setIR 1750,5000,0,6
@18:18:16.15 Gate.setIR 0,5000,0,6
@18:18:16.21 PuckHeater.configure :default
@18:18:16.25 Gate.power :heat,:ON,limit to 5000mA
@18:18:16.92 Mounted lysis Cartridge board#Q05076
@18:18:16.95 Using default puck heat loss curve for HeatingAccelInCarousel
@18:18:36.06 -> Cmd.startFiltering
@18:18:36.51 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@18:18:36.53 SamplePump.setPosition! 0ml
@18:18:36.57 Bypass.open
@18:18:38.06 Intake.open
@18:18:38.84 Sea 7.0m deep (25.1psia)
@18:18:38.86 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@18:18:41.44 Vacuum tare is 51% of full scale
@18:18:41.56 Pressure.in :default
@18:18:41.62 Pressure.hold
@18:18:41.79 SamplePump.in :prime
@18:18:41.86 SamplePump.seek 10ml,1:21
@18:18:51.24 Pressure.coast
@18:18:51.29 SamplePump.setPosition! 0ml
@18:18:51.34 Exhaust.open
@18:18:52.78 <closeBypass> Bypass.close
@18:18:54.32 <FILTERING> Pressure.in :default
@18:18:54.38 Pressure.hold
@18:18:54.48 Sea 6.8m deep (24.7psia)
@18:18:54.61 SamplePump.in :prime
@18:18:54.68 SamplePump.seek 100ml,8:51
@18:21:04.47 Pressure.coast
@18:21:04.53 Bypass.open
@18:21:05.24 Intake.close
@18:21:06.08 Toroid.in :hold
@18:21:06.14 Toroid.seek :crack
@18:21:07.69 Toroid.seek :cartridge
@18:21:08.78 Toroid.in :default
@18:21:08.84 Toroid.seek :clear
@18:21:10.80 Pressurizing cartridge to 26psi above 24.8psia
@18:21:10.81 SamplePump.setPosition! 0ml
@18:21:10.85 Intake.open
@18:21:11.58 Bypass.close
@18:21:12.33 Exhaust.close
@18:21:13.17 Pressure.in :default
@18:21:13.23 Pressure.hold
@18:21:13.39 SamplePump.in :charge
@18:21:13.46 SamplePump.seek 2.3ml,42 seconds
@18:21:22.26 Pressure.coast
@18:21:22.31 Intake.close
@18:21:23.13 Pressurized cartridge with 0.344ml to 26.3psi
@18:21:23.14 Isolated @46.4psia (21.6psi)
@18:21:23.16 Verifying cartridge holds at least 0.75..0.85 of 46.4psia
@18:21:23.19 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@18:21:27.81 Sampling 1000.0ml
@18:21:27.83 SamplePump.setPosition! 0ml
@18:21:27.88 Exhaust.open
@18:21:28.58 Intake.open
@18:21:29.41 Pressure.in :default
@18:21:29.47 Pressure.hold
@18:21:29.59 Sea 7.1m deep (25.1psia)
@18:21:29.72 SamplePump.in :sample
@18:21:29.79 SamplePump.seek 1000ml,1:23:53
@18:21:32.45 <sampling> Cartridge 33 filtering 1/1000ml at 0.34ml/s with 24.6psi vacuum 5.8m deep (23.3psia)
@18:22:32.44 Cartridge 33 filtering 19/1000ml at 0.26ml/s with 26.2psi vacuum
@18:23:32.86 Cartridge 33 filtering 37/1000ml at 0.27ml/s with 27.2psi vacuum
@18:24:32.44 Cartridge 33 filtering 54/1000ml at 0.25ml/s with 20.2psi vacuum 4.0m deep (20.6psia)
@18:25:32.45 Cartridge 33 filtering 70/1000ml at 0.27ml/s with 28.6psi vacuum 2.5m deep (18.3psia)
@18:26:32.44 Cartridge 33 filtering 87/1000ml at 0.24ml/s with 24.0psi vacuum 3.6m deep (20.0psia)
@18:27:32.45 Cartridge 33 filtering 102/1000ml at 0.29ml/s with 24.5psi vacuum 3.2m deep (19.4psia)
@18:28:32.44 Cartridge 33 filtering 118/1000ml at 0.21ml/s with 21.6psi vacuum 4.7m deep (21.6psia)
@18:29:32.45 Cartridge 33 filtering 133/1000ml at 0.24ml/s with 21.1psi vacuum 2.8m deep (18.9psia)
@18:30:32.44 Cartridge 33 filtering 147/1000ml at 0.24ml/s with 21.9psi vacuum 4.6m deep (21.5psia)
@18:31:32.45 Cartridge 33 filtering 161/1000ml at 0.23ml/s with 23.0psi vacuum 3.3m deep (19.5psia)
@18:32:32.44 Cartridge 33 filtering 175/1000ml at 0.20ml/s with 23.8psi vacuum 2.3m deep (18.1psia)
@18:33:32.45 Cartridge 33 filtering 189/1000ml at 0.20ml/s with 24.4psi vacuum 4.3m deep (21.1psia)
@18:34:32.44 Cartridge 33 filtering 202/1000ml at 0.21ml/s with 22.2psi vacuum 2.3m deep (18.1psia)
@18:35:32.45 Cartridge 33 filtering 216/1000ml at 0.23ml/s with 24.1psi vacuum 4.3m deep (21.0psia)
@18:36:32.44 Cartridge 33 filtering 228/1000ml at 0.20ml/s with 26.9psi vacuum 2.4m deep (18.2psia)
@18:37:32.45 Cartridge 33 filtering 241/1000ml at 0.20ml/s with 26.7psi vacuum 2.9m deep (19.0psia)
@18:38:32.44 Cartridge 33 filtering 254/1000ml at 0.20ml/s with 25.5psi vacuum 3.9m deep (20.4psia)
@18:39:32.45 Cartridge 33 filtering 266/1000ml at 0.20ml/s with 28.0psi vacuum
@18:40:32.44 Cartridge 33 filtering 278/1000ml at 0.20ml/s with 25.9psi vacuum 3.6m deep (19.9psia)
@18:41:09.70 <FILTERING> Flow averaged 0.24ml/s over 19:40
@18:41:09.73 Waiting up to 12 seconds for vacuum to reduce from 27.4 to 18.0psi
@18:41:10.30 Relieved excess vacuum in .6 seconds
@18:41:10.33 SamplePump.seek 1000ml,1:00:03
@18:41:32.45 <sampling> Cartridge 33 filtering 290/1000ml at 0.21ml/s with 24.4psi vacuum 2.8m deep (18.8psia)
@18:41:39.20 <FILTERING> Flow averaged 0.20ml/s over 29 seconds
@18:41:39.23 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi
@18:41:39.80 Relieved excess vacuum in .6 seconds
@18:41:39.83 SamplePump.seek 1000ml,59:33
@18:41:44.51 Flow estimate reduced to 0.16ml/s
@18:41:44.53 <closeIntake> Intake.close
@18:41:44.60 <closeExhaust> Exhaust.close
@18:41:44.77 <FILTERING> Toroid.in :hold
@18:41:44.83 Toroid.seek :bypass
@18:41:46.46 Toroid.in :default
@18:41:46.52 Toroid.seek :clear
@18:41:48.13 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@18:41:48.14 Pressure.coast
@18:41:48.33 Sampled  292.7ml
@18:41:50.31 <LRAUV> -> Cmd.startProcessing
@18:41:51.74 <PROCESSING> SPR.command \"read_sequences\"
@18:41:52.06 SPR.command \"stats_on\"
@18:41:52.10 SPR.command \"start 0 1\"
@18:41:54.11 <SPRlogger> Begun log /var/log/esp/spr21apr2218h.spr
@18:41:54.91 <PROCESSING> Handoff.select :waste
@18:42:01.59 Syringe tare is 20% of full scale
@18:42:01.61 PinPort.in :locking
@18:42:01.68 PinPort.seek :locked
@18:42:02.16 <posArchive> Archive.in :fast
@18:42:02.22 <posLyS2> Lysis2.in :fast
@18:42:02.27 <PROCESSING> Lysis1.seek 1350uL
@18:42:02.38 <posArchive> Archive.seek :full
@18:42:02.43 <posLyS2> Lysis2.seek :full
@18:42:04.51 <PROCESSING> Plunge.seek :reagent
@18:42:05.42 Evacuating puck with filter bubble point of 50psi
@18:42:05.44 PinPort.seek :locked
@18:42:05.52 Handoff.select :waste
@18:42:12.10 Air syringe pumping up to 1 stroke to reach 8psi
@18:42:12.11 Air stroke #1 of 1
@18:42:12.20 Air.in :pressurize
@18:42:12.26 Air.seek :pressurized
@18:42:23.52 0.1psi after pressurize
@18:42:23.53 Air.in :compress
@18:42:23.60 Air.seek :evac
@18:42:27.69 0.6psi after compress
@18:42:40.26 0.2psi after compress (stable)
@18:42:40.27 Air.in :default
@18:42:40.34 Air.hold
@18:42:40.51 at 0.1psi Opened to waste
@18:42:40.52 Handoff.select :blocked
@18:42:40.65 Archive syringe pumping up to 5 strokes to reach 50psi
@18:42:40.66 Archive stroke #1 of 5
@18:42:40.73 Archive.in :pressurize
@18:42:40.82 Archive.seek :pressurized
@18:42:53.56 11.1psi after pressurize
@18:42:53.57 Archive.in :compress
@18:42:53.65 Archive.seek :empty
@18:43:09.53 31.2psi after compress
@18:43:23.10 36.1psi after compress (stable)
@18:43:23.12 Archive.in :gulp
@18:43:23.19 Archive.seek :topVent
@18:43:30.39 36.3psi after gulp
@18:43:42.96 36.2psi after gulp (stable)
@18:43:42.97 Archive stroke #2 of 5
@18:43:43.06 Archive.in :pressurize
@18:43:43.12 Archive.seek :pressurized
@18:43:55.87 36.1psi after pressurize
@18:43:55.88 Archive.in :compress
@18:43:55.96 Archive.seek :empty
@18:44:09.40 Reached 50.2 of 50.0psi after pumping 3408uL (1.97 strokes)
@18:44:09.41 Archive.in :default
@18:44:09.48 Archive.hold
@18:44:09.54 Handoff.select :waste
@18:44:19.74 delay 1:00
@18:45:19.76 Handoff.select :blocked
@18:45:42.44 Handoff.select :waste
@18:45:42.56 0.0psi at evacEnd
@18:45:42.57 Handoff.select :blocked
@18:45:42.69 Archive.in :fast
@18:45:42.76 Archive.seek :topVent
@18:45:56.28 Stable @0.6psi after Handoff blocked
@18:45:56.30 Archive syringe pumping up to 5 strokes to reach 31psi
@18:45:56.31 Archive stroke #1 of 5
@18:45:56.41 Archive.in :pressurize
@18:45:56.47 Archive.seek :pressurized
@18:46:09.20 0.7psi after pressurize
@18:46:09.21 Archive.in :compress
@18:46:09.28 Archive.seek :empty
@18:46:25.17 2.7psi after compress
@18:47:04.74 24.5psi after compress (stable)
@18:47:04.76 Archive.in :gulp
@18:47:04.83 Archive.seek :topVent
@18:47:12.03 26.0psi after gulp
@18:47:24.61 26.6psi after gulp (stable)
Archive stroke #2 of 5
@18:47:24.69 Archive.in :pressurize
@18:47:24.76 Archive.seek :pressurized
@18:47:37.49 27.4psi after pressurize
@18:47:37.50 Archive.in :compress
@18:47:37.57 Archive.seek :empty
@18:47:53.45 30.0psi after compress
@18:47:54.04 Reached 30.7 of 30.6psi after pumping 3460uL (2.00 strokes)
@18:47:54.05 Archive.in :default
@18:47:54.11 Archive.hold
@18:47:54.18 Handoff.select :waste
@18:48:04.36 delay 1:00
@18:49:04.38 Handoff.select :blocked
@18:49:27.05 Handoff.select :waste
@18:49:27.17 1.1psi at evacEnd
@18:49:27.19 Air.seek :fullVent
@18:49:28.64 Archive.in :fast
@18:49:28.70 Archive.seek :topVent
@18:49:35.84 Seawater evacuated, Presure above/below filter released. Lysis begins
@18:49:35.93 Lysis1.in :slow
@18:49:36.00 Lysis1.seek 1050uL
@18:50:06.03 delay 5 seconds
@18:50:11.05 Handoff.select :blocked
@18:50:11.21 Lysis1.seek 450uL
@18:51:11.18 delay 3 seconds
@18:51:14.25 Air.in to mix into 1.37806 parts Lysis1
@18:51:14.76 <Airmixer> Air.seek :evac
@18:51:14.79 <PROCESSING> Lysis1.seek 300uL
@18:51:29.91 Lysis1.seek :empty
@18:52:00.01 Lysis buffer delivered @22.8psi
@18:52:00.02 Polling Can every 2:00
@18:52:00.57 PuckHeater.configure :default #ambient 11.7C
@18:52:00.61 PuckHeater.seek 80.2C
@18:52:52.27 PuckHeater.hold 2:00,80.2C
@18:54:52.24 Polling Can every 2:00
@18:54:52.79 PuckHeater.configure :default #ambient 11.7C
@18:54:52.85 PuckHeater.hold 30 seconds,79.2C
@18:55:23.00 Polling Can every 2:00
@18:55:23.54 PuckHeater.configure :default #ambient 11.7C
@18:55:23.58 PuckHeater.hold 30 seconds,78.6C
@18:55:53.72 Polling Can every 2:00
@18:55:54.27 PuckHeater.configure :default #ambient 11.7C
@18:55:54.33 PuckHeater.hold 10:00,78.1C
@19:05:53.48 Lysis complete. Syringe pressure @27.8psi
@19:05:53.49 Handoff.select :blocked
@19:05:53.53 Archive syringe pumping up to 3 strokes to reach 40psi
@19:05:53.55 Archive stroke #1 of 3
@19:05:53.64 Archive.in :pressurize
@19:05:53.70 Archive.seek :pressurized
@19:06:06.44 27.3psi after pressurize
@19:06:06.45 Archive.in :compress
@19:06:06.52 Archive.seek :empty
@19:06:15.86 Reached 40.9 of 40.0psi after pumping 1590uL (0.92 strokes)
@19:06:15.87 Archive.in :default
@19:06:15.93 Archive.hold
@19:06:15.99 delay 2 seconds
@19:06:18.02 Archive.in :fast
@19:06:18.09 Archive.seek :full
@19:06:24.80 Lysis1.seek 138uL
@19:06:38.68 delay 1 second
@19:06:39.71 <fillArchive> Archive.seek :full
@19:06:39.74 <PROCESSING> Lysis1.seek :empty
@19:06:53.71 Initial air pressure @48.8psi
@19:06:53.79 Lysis1.in :extract
@19:06:54.23 Lysis1.seek 600uL,nil
@19:06:57.19 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@19:06:57.20 Archive stroke #1 of 6
@19:06:57.30 Archive.in :pressurize
@19:06:57.38 Archive.seek :pressurized
@19:07:10.11 15.7psi after pressurize
@19:07:10.12 Archive.in :compress
@19:07:10.21 Archive.seek :empty
@19:07:25.17 Archive.in :default
@19:07:25.22 Archive.hold
@19:07:25.44 <PROCESSING> Initial 600uL lysate @19.0psi
@19:07:25.45 Handoff.select :blocked
@19:07:25.54 Initial air pressure @20.3psi
@19:07:25.62 Lysis1.seek 825uL,nil
@19:07:27.00 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@19:07:27.01 Archive stroke #1 of 6
@19:07:27.10 Archive.in :compress
@19:07:27.16 Archive.seek :empty
@19:07:27.77 Reached 17.2 of 17.0psi after pumping 1724uL (1.00 strokes)
@19:07:27.78 Archive.in :default
@19:07:27.85 Archive.hold
@19:07:28.60 Archive syringe pumping up to 6 strokes to reach 17psi
@19:07:28.61 Archive stroke #1 of 6
@19:07:28.70 Archive.in :compress
@19:07:28.77 Archive.seek :empty
@19:07:29.27 16.2psi after compress
@19:07:36.06 Archive.in :default
@19:07:36.11 Archive.hold
@19:07:36.32 <PROCESSING> Recovered 825uL lysate @16.4psi
@19:07:36.34 Air.in :halfSpeed
@19:07:36.40 Air.seek :fullVent
@19:07:38.65 Puck Top Vented, bottom @1.1psi
@19:07:38.66 Handoff.select :waste
@19:07:38.78 delay 5 seconds
@19:07:43.84 Handoff open to waste, bottom @0.1psi
@19:07:44.30 Air.in :default
@19:07:44.37 Air.seek :evac
@19:07:45.80 Lysis2.in :DAmix
@19:07:45.90 Lysis1.in :DAmix
@19:07:46.23 <Lysis2mixer> Lysis2.seek 1480uL
@19:07:46.28 <PROCESSING> Lysis1.seek 575uL
@19:08:21.35 delay 5 seconds
@19:08:26.37 Handoff.select SPR
@19:08:26.49 SPR.command \"start 2 14\"
@19:08:27.79 delay 10 seconds
@19:08:37.81 SPR.command \"start 1 0\"
@19:08:41.05 Waiting up to 4:00 for SPR to enter state 1
@19:10:43.42 SPR.command \"hold_on\"
@19:10:44.83 SPR Dilution 1 of 1 -- Running Experiment 1
@19:10:45.25 <Lysis2mixer> Lysis2.seek 1180uL
@19:10:45.33 <PROCESSING> Lysis1.seek 275uL
@19:11:27.33 volL1 = 300.0 and volL2 = 300.0
@19:11:27.34 delay 15 seconds
@19:11:42.36 SPR.command \"hold_off\"
@19:11:45.97 Waiting up to 38:00 for SPR to enter state 7
@19:38:29.97 SPR Analysis has Completed
@19:38:29.99 Handoff.select :waste
@19:38:30.91 <SPRlogger> Ended log /var/log/esp/spr21apr2218h.spr
@19:38:30.92 <PROCESSING> SPR.command \"stats_off\"
@19:38:32.57 <LRAUV> -> Cmd.stop
@19:38:32.66 <closeHandoff> Handoff.select :close
@19:38:32.78 <LRAUV> Gate.power :heat,:OFF
@19:38:32.80 Gate.power :cartridge,:OFF
@19:38:32.88 Carousel.seek 33
@19:38:33.05 Plunge.seek :locked
@19:38:33.93 PinPort.in :unlocking
@19:38:33.99 PinPort.seek :clear
@19:38:34.52 <Archive> Archive.in :unlocking
@19:38:34.65 Archive.seek :clear
@19:38:34.72 <Air> Air.in :unlocking
@19:38:34.83 Air.seek :clear
@19:38:34.86 <Lysis1> Lysis1.in :unlocking
@19:38:35.05 Lysis1.seek :clear
@19:38:35.08 <Lysis2> Lysis2.in :unlocking
@19:38:35.19 Lysis2.seek :clear
@19:38:36.58 Slide::Error in LRAUV -- Plunge Valve (PV) positionErr at locked (sensed locked - 79 counts)
@19:38:36.61 <LRAUV> Archive.coast
@19:38:36.69 Air.coast
@19:38:36.75 Lysis1.coast
@19:38:36.80 Lysis2.coast
@19:38:36.87 Plunge.coast
@19:38:36.92 SamplePump.coast
@19:38:36.98 Toroid.coast
@19:38:37.03 Carousel.coast
@19:38:37.08 PinPort.coast
@19:38:37.36 Gate.power :motor,:OFF
@19:38:37.38 Gate.power :core,:OFF
@19:38:37.40 Gate.power :analytic1,:OFF
@19:38:37.42 Gate.power :analytic2,:OFF
@19:38:37.44 Gate.power :analytic3,:OFF
@19:38:37.46 Gate.power :main,:OFF
@19:38:37.48 ready to power off
##-- file closed 2021-04-23T04:45:34.841Z

##-- file opened 2021-04-23T04:45:34.843Z
@21:45:35.52 -> showStatus
@21:45:37.54 -> Cmd.loadCartridge -6
@21:45:37.68 Gate.power :main,:ON,limit to 5000mA
@21:45:37.70 Gate.power :analytic1,:ON,limit to 5000mA
@21:45:37.72 Gate.power :analytic2,:ON,limit to 5000mA
@21:45:37.74 Gate.power :analytic3,:ON,limit to 5000mA
@21:45:37.88 Powering core bus
@21:45:40.16 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@21:45:40.29 <LRAUV> Archive.configure :default
@21:45:40.42 Air.configure :default
@21:45:40.52 Lysis1.configure :default
@21:45:40.62 Lysis2.configure :default
@21:45:40.75 Plunge.configure :default
@21:45:40.87 SamplePump.configure :default
@21:45:41.02 ExtraPump.configure :default
@21:45:41.12 Toroid.configure :default
@21:45:41.25 Carousel.configure :default
@21:45:41.38 Pressure.configure :default
@21:45:41.49 PinPort.configure :default
@21:45:41.56 All elf controllers running firmware version 4.741
@21:45:41.59 Gate.power :motor,:ON,limit to 5000mA
@21:45:41.69 <Archive_home> Archive.in :default
@21:45:42.05 Archive.home.jog 0
@21:45:42.21 <Toroid_home> Toroid.in :default
@21:45:42.28 <Plunge_home> Plunge.in :default
@21:45:42.32 <Lysis1_home> Lysis1.in :default
@21:45:42.35 <Air_home> Air.in :default
@21:45:42.40 <PinPort_home> PinPort.in :default
@21:45:42.86 <Lysis2_home> Lysis2.in :default
@21:45:42.97 <Toroid_home> Toroid.home.jog 0
@21:45:43.05 <Lysis1_home> Lysis1.home.jog 0
@21:45:43.11 <Plunge_home> Plunge.home.jog 0
@21:45:43.14 <Air_home> Air.home.jog 0
@21:45:43.18 <PinPort_home> PinPort.home.jog 0
@21:45:43.20 <Lysis2_home> Lysis2.home.jog 0
@21:45:43.59 <Archive_home> Archive.in :homing
@21:45:43.75 Archive.jog 4943
@21:45:43.91 <PinPort_home> PinPort.in :homing
@21:45:43.95 <Toroid_home> Toroid.in :homing
@21:45:43.99 <Lysis1_home> Lysis1.in :homing
@21:45:44.04 <Lysis2_home> Lysis2.in :homing
@21:45:44.06 <Air_home> Air.in :homing
@21:45:44.12 <Plunge_home> Plunge.in :homing
@21:45:44.27 <PinPort_home> PinPort.jog -107
@21:45:44.31 <Toroid_home> Toroid.jog 608
@21:45:44.35 <Lysis2_home> Lysis2.jog 151
@21:45:44.38 <Lysis1_home> Lysis1.jog 3998
@21:45:44.42 <Air_home> Air.jog 4004
@21:45:44.47 <Plunge_home> Plunge.jog 1580
@21:45:44.67 <PinPort_home> PinPort.setRawPosition! 0
@21:45:44.71 PinPort.seek :home
@21:45:44.79 PinPort.in :default
@21:45:44.93 <Lysis2_home> Lysis2.setRawPosition! 50
@21:45:44.98 Lysis2.seek :home
@21:45:45.25 Lysis2.in :default
@21:45:45.81 <Plunge_home> Plunge.setRawPosition! 50
@21:45:45.85 Plunge.seek :home
@21:45:46.13 Plunge.in :default
@21:45:46.35 <Archive_home> Archive.setRawPosition! 50
@21:45:46.39 Archive.seek :home
@21:45:46.51 <Toroid_home> Toroid.setRawPosition! 0
@21:45:46.56 Toroid.seek :home
@21:45:46.64 Toroid.in :default
@21:45:46.74 <Archive_home> Archive.in :default
@21:45:46.83 <Lysis1_home> Lysis1.setRawPosition! 50
@21:45:46.84 <Air_home> Air.setRawPosition! 50
@21:45:46.91 <Lysis1_home> Lysis1.seek :home
@21:45:46.97 <Air_home> Air.seek :home
@21:45:47.22 <Lysis1_home> Lysis1.in :default
@21:45:47.31 <Air_home> Air.in :default
@21:45:47.67 <LRAUV> Toroid.seek :clear
@21:45:49.29 Carousel.in :default
@21:45:49.35 Carousel.home.jog 0
@21:45:49.84 Carousel.in :default
@21:45:49.90 Carousel.seek 33
@21:45:50.06 Selecting Cartridge 34
@21:45:50.30 Carousel.seek 34
@21:45:50.79 Lysis1.in :fast
@21:45:50.85 Lysis1.seek :locked
@21:45:53.44 <Plunge> Plunge.seek :locked
@21:45:53.59 <Air> Air.in :fast
@21:45:53.69 Air.seek :locked
@21:45:56.29 <LRAUV> Powering cartridge bus
@21:45:58.41 Gate.setIR 1750,5000,0,6
@21:45:58.54 Gate.setIR 0,5000,0,6
@21:45:58.59 Gate.power :cartridge,:OFF
@21:45:59.12 Mounted archive Cartridge
@21:46:17.14 -> Cmd.startFiltering
@21:46:17.59 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@21:46:17.61 SamplePump.setPosition! 0ml
@21:46:17.65 Bypass.open
@21:46:19.16 Intake.open
@21:46:19.97 Sea 6.4m deep (24.2psia)
@21:46:19.98 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@21:46:22.57 Vacuum tare is 51% of full scale
@21:46:22.70 Pressure.in :default
@21:46:22.78 Pressure.hold
@21:46:22.99 SamplePump.in :prime
@21:46:23.05 SamplePump.seek 10ml,1:21
@21:46:32.44 Pressure.coast
@21:46:32.49 SamplePump.setPosition! 0ml
@21:46:32.54 Exhaust.open
@21:46:33.97 <closeBypass> Bypass.close
@21:46:35.53 <FILTERING> Pressure.in :default
@21:46:35.59 Pressure.hold
@21:46:35.69 Sea 6.1m deep (23.7psia)
@21:46:35.82 SamplePump.in :prime
@21:46:35.90 SamplePump.seek 100ml,8:51
@21:48:46.03 Pressure.coast
@21:48:46.09 Bypass.open
@21:48:46.79 Intake.close
@21:48:47.66 Toroid.in :hold
@21:48:47.72 Toroid.seek :crack
@21:48:49.27 Toroid.seek :cartridge
@21:48:50.36 Toroid.in :default
@21:48:50.42 Toroid.seek :clear
@21:48:52.38 Pressurizing cartridge to 26psi above 23.6psia
@21:48:52.39 SamplePump.setPosition! 0ml
@21:48:52.43 Intake.open
@21:48:53.19 Bypass.close
@21:48:53.90 Exhaust.close
@21:48:54.70 Pressure.in :default
@21:48:54.76 Pressure.hold
@21:48:54.92 SamplePump.in :charge
@21:48:54.99 SamplePump.seek 2.3ml,42 seconds
@21:49:01.01 Pressure.coast
@21:49:01.06 Intake.close
@21:49:01.88 Pressurized cartridge with 0.160ml to 26.6psi
@21:49:01.89 Isolated @43.8psia (20.2psi)
@21:49:01.91 Verifying cartridge holds at least 0.75..0.85 of 43.8psia
@21:49:01.94 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@21:49:06.56 Sampling 1000.0ml
@21:49:06.58 SamplePump.setPosition! 0ml
@21:49:06.63 Exhaust.open
@21:49:07.35 Intake.open
@21:49:08.19 Pressure.in :default
@21:49:08.25 Pressure.hold
@21:49:08.37 Sea 5.5m deep (22.8psia)
@21:49:08.50 SamplePump.in :sample
@21:49:08.57 SamplePump.seek 1000ml,1:23:53
@21:49:11.23 <sampling> Cartridge 34 filtering 1/1000ml at 0.27ml/s with 27.4psi vacuum 1.7m deep (17.3psia)
@21:50:11.23 Cartridge 34 filtering 19/1000ml at 0.27ml/s with 29.3psi vacuum 1.7m deep (17.2psia)
@21:51:11.23 Cartridge 34 filtering 37/1000ml at 0.25ml/s with 24.0psi vacuum 2.1m deep (17.8psia)
@21:52:11.63 Cartridge 34 filtering 54/1000ml at 0.28ml/s with 21.3psi vacuum 3.6m deep (19.9psia)
@21:53:11.23 Cartridge 34 filtering 70/1000ml at 0.28ml/s with 25.9psi vacuum 4.7m deep (21.6psia)
@21:54:11.23 Cartridge 34 filtering 86/1000ml at 0.23ml/s with 27.6psi vacuum
@21:55:11.23 Cartridge 34 filtering 102/1000ml at 0.29ml/s with 23.9psi vacuum 4.8m deep (21.8psia)
@21:56:11.23 Cartridge 34 filtering 117/1000ml at 0.22ml/s with 22.4psi vacuum 1.8m deep (17.3psia)
@21:57:11.23 Cartridge 34 filtering 132/1000ml at 0.27ml/s with 24.0psi vacuum 5.2m deep (22.3psia)
@21:58:11.23 Cartridge 34 filtering 147/1000ml at 0.27ml/s with 22.1psi vacuum 3.8m deep (20.2psia)
@21:59:11.23 Cartridge 34 filtering 161/1000ml at 0.25ml/s with 27.4psi vacuum 3.5m deep (19.8psia)
@22:00:11.23 Cartridge 34 filtering 175/1000ml at 0.20ml/s with 25.1psi vacuum
@22:01:11.23 Cartridge 34 filtering 189/1000ml at 0.24ml/s with 24.4psi vacuum 3.9m deep (20.5psia)
@22:02:11.23 Cartridge 34 filtering 203/1000ml at 0.20ml/s with 24.3psi vacuum 2.7m deep (18.7psia)
@22:03:11.23 Cartridge 34 filtering 216/1000ml at 0.21ml/s with 27.0psi vacuum 2.7m deep (18.7psia)
@22:04:11.23 Cartridge 34 filtering 229/1000ml at 0.20ml/s with 26.8psi vacuum 3.2m deep (19.4psia)
@22:05:11.23 Cartridge 34 filtering 242/1000ml at 0.21ml/s with 24.6psi vacuum 2.4m deep (18.2psia)
@22:06:11.23 Cartridge 34 filtering 255/1000ml at 0.21ml/s with 23.7psi vacuum 5.1m deep (22.3psia)
@22:07:11.23 Cartridge 34 filtering 267/1000ml at 0.23ml/s with 23.7psi vacuum 2.6m deep (18.5psia)
@22:08:11.23 Cartridge 34 filtering 280/1000ml at 0.20ml/s with 23.7psi vacuum 2.7m deep (18.6psia)
@22:09:11.23 Cartridge 34 filtering 292/1000ml at 0.20ml/s with 28.3psi vacuum 1.6m deep (17.0psia)
@22:10:11.23 Cartridge 34 filtering 304/1000ml at 0.20ml/s with 26.2psi vacuum 3.9m deep (20.4psia)
@22:10:35.11 <FILTERING> Flow averaged 0.24ml/s over 21:27
@22:10:35.14 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi
@22:10:35.71 Relieved excess vacuum in .6 seconds
@22:10:35.73 SamplePump.seek 1000ml,58:06
@22:10:38.95 Flow estimate reduced to 0.19ml/s
@22:10:38.97 <closeIntake> Intake.close
@22:10:39.03 <closeExhaust> Exhaust.close
@22:10:39.20 <FILTERING> Toroid.in :hold
@22:10:39.26 Toroid.seek :bypass
@22:10:40.89 Toroid.in :default
@22:10:40.95 Toroid.seek :clear
@22:10:42.56 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@22:10:42.57 Pressure.coast
@22:10:42.76 Sampled  309.9ml
@22:10:44.93 <LRAUV> -> Cmd.startProcessing
@22:10:45.30 <PROCESSING> Plunge.seek :reagent
@22:10:46.14 Sample loop closed. Ready to Deliver Reagents
@22:10:46.15 Archive.seek :sealed
@22:10:50.42 Archive.seek :evac
@22:11:04.35 delay 1:00
@22:12:04.37 Archive.seek :clear
@22:12:22.09 RNAlater soak for 10:00
@22:22:22.12 Archive.seek :tVent
@22:22:24.70 delay 1 second
@22:22:25.72 Archive.seek :evac
@22:22:41.34 delay 10 seconds
@22:22:51.37 Sample preserved and evacuated
@22:22:52.56 <LRAUV> -> Cmd.stop
@22:22:52.70 Carousel.seek 34
@22:22:52.87 Plunge.seek :locked
@22:22:53.82 <Archive> Archive.in :unlocking
@22:22:53.94 Archive.seek :clear
@22:22:54.02 <Air> Air.in :unlocking
@22:22:54.13 Air.seek :clear
@22:22:54.20 <Lysis1> Lysis1.in :unlocking
@22:22:54.31 Lysis1.seek :clear
@22:22:57.98 <LRAUV> Archive.coast
@22:22:58.03 Air.coast
@22:22:58.08 Lysis1.coast
@22:22:58.13 Lysis2.coast
@22:22:58.18 Plunge.coast
@22:22:58.23 SamplePump.coast
@22:22:58.28 Toroid.coast
@22:22:58.33 Carousel.coast
@22:22:58.38 PinPort.coast
@22:22:58.56 Gate.power :motor,:OFF
@22:22:58.58 Gate.power :core,:OFF
@22:22:58.60 Gate.power :analytic1,:OFF
@22:22:58.62 Gate.power :analytic2,:OFF
@22:22:58.64 Gate.power :analytic3,:OFF
@22:22:58.66 Gate.power :main,:OFF
@22:22:58.68 Safely stopped and ready to power off
##-- file closed 2021-04-23T05:34:30.782Z

##-- file opened 2021-04-23T05:34:30.790Z
@22:34:31.43 -> showStatus
@22:34:33.45 -> Cmd.loadCartridge -9
@22:34:33.60 Gate.power :main,:ON,limit to 5000mA
@22:34:33.61 Gate.power :analytic1,:ON,limit to 5000mA
@22:34:33.63 Gate.power :analytic2,:ON,limit to 5000mA
@22:34:33.65 Gate.power :analytic3,:ON,limit to 5000mA
@22:34:33.78 Powering core bus
@22:34:36.06 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@22:34:36.19 <LRAUV> Archive.configure :default
@22:34:36.31 Air.configure :default
@22:34:36.41 Lysis1.configure :default
@22:34:36.52 Lysis2.configure :default
@22:34:36.64 Plunge.configure :default
@22:34:36.76 SamplePump.configure :default
@22:34:36.91 ExtraPump.configure :default
@22:34:37.02 Toroid.configure :default
@22:34:37.14 Carousel.configure :default
@22:34:37.26 Pressure.configure :default
@22:34:37.36 PinPort.configure :default
@22:34:37.43 All elf controllers running firmware version 4.741
@22:34:37.45 Gate.power :motor,:ON,limit to 5000mA
@22:34:37.59 <Archive_home> Archive.in :default
@22:34:37.89 Archive.home.jog 0
@22:34:38.10 <Toroid_home> Toroid.in :default
@22:34:38.52 <Plunge_home> Plunge.in :default
@22:34:38.58 <Lysis1_home> Lysis1.in :default
@22:34:38.63 <PinPort_home> PinPort.in :default
@22:34:38.69 <Lysis2_home> Lysis2.in :default
@22:34:38.72 <Air_home> Air.in :default
@22:34:38.92 <Toroid_home> Toroid.home.jog 0
@22:34:38.94 <Plunge_home> Plunge.home.jog 0
@22:34:38.97 <Lysis1_home> Lysis1.home.jog 0
@22:34:39.00 <Lysis2_home> Lysis2.home.jog 0
@22:34:39.02 <PinPort_home> PinPort.home.jog 0
@22:34:39.10 <Air_home> Air.home.jog 0
@22:34:39.48 <Archive_home> Archive.in :homing
@22:34:39.64 Archive.jog 156
@22:34:39.80 <PinPort_home> PinPort.in :homing
@22:34:39.84 <Toroid_home> Toroid.in :homing
@22:34:39.88 <Lysis1_home> Lysis1.in :homing
@22:34:39.93 <Lysis2_home> Lysis2.in :homing
@22:34:39.95 <Air_home> Air.in :homing
@22:34:39.98 <Plunge_home> Plunge.in :homing
@22:34:40.18 <PinPort_home> PinPort.jog -107
@22:34:40.23 <Toroid_home> Toroid.jog 601
@22:34:40.26 <Lysis2_home> Lysis2.jog 151
@22:34:40.28 <Air_home> Air.jog 158
@22:34:40.31 <Lysis1_home> Lysis1.jog 1416
@22:34:40.35 <Plunge_home> Plunge.jog 1578
@22:34:40.58 <PinPort_home> PinPort.setRawPosition! 0
@22:34:40.60 <Archive_home> Archive.setRawPosition! 50
@22:34:40.68 <PinPort_home> PinPort.seek :home
@22:34:40.71 <Archive_home> Archive.seek :home
@22:34:40.83 <PinPort_home> PinPort.in :default
@22:34:40.92 <Lysis2_home> Lysis2.setRawPosition! 50
@22:34:40.95 <Air_home> Air.setRawPosition! 50
@22:34:41.04 <Lysis2_home> Lysis2.seek :home
@22:34:41.07 <Air_home> Air.seek :home
@22:34:41.20 <Archive_home> Archive.in :default
@22:34:41.39 <Lysis2_home> Lysis2.in :default
@22:34:41.41 <Air_home> Air.in :default
@22:34:41.64 <Lysis1_home> Lysis1.setRawPosition! 50
@22:34:41.69 Lysis1.seek :home
@22:34:41.78 <Plunge_home> Plunge.setRawPosition! 50
@22:34:41.82 Plunge.seek :home
@22:34:41.97 <Lysis1_home> Lysis1.in :default
@22:34:42.10 <Plunge_home> Plunge.in :default
@22:34:42.43 <Toroid_home> Toroid.setRawPosition! 0
@22:34:42.47 Toroid.seek :home
@22:34:42.57 Toroid.in :default
@22:34:42.69 <LRAUV> Toroid.seek :clear
@22:34:44.30 Carousel.in :default
@22:34:44.36 Carousel.home.jog 0
@22:34:44.85 Carousel.in :default
@22:34:44.91 Carousel.seek 34
@22:34:45.04 Selecting Cartridge 31
@22:34:45.28 Carousel.seek 31
@22:34:46.17 Lysis1.in :fast
@22:34:46.23 Lysis1.seek :locked
@22:34:48.83 <Plunge> Plunge.seek :locked
@22:34:48.96 <Air> Air.in :fast
@22:34:49.02 Air.seek :locked
@22:34:51.62 <LRAUV> Powering cartridge bus
@22:34:53.74 Gate.setIR 1750,5000,0,6
@22:34:53.87 Gate.setIR 0,5000,0,6
@22:34:53.93 PuckHeater.configure :default
@22:34:53.98 Gate.power :heat,:ON,limit to 5000mA
@22:34:54.64 Mounted lysis Cartridge board#Q05080
@22:34:54.67 Using default puck heat loss curve for HeatingAccelInCarousel
@22:35:13.86 -> Cmd.startFiltering
@22:35:14.31 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@22:35:14.33 SamplePump.setPosition! 0ml
@22:35:14.37 Bypass.open
@22:35:15.82 Intake.open
@22:35:16.63 Sea 6.4m deep (24.1psia)
@22:35:16.65 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@22:35:19.23 Vacuum tare is 51% of full scale
@22:35:19.35 Pressure.in :default
@22:35:19.41 Pressure.hold
@22:35:19.65 SamplePump.in :prime
@22:35:19.71 SamplePump.seek 10ml,1:21
@22:35:29.11 Pressure.coast
@22:35:29.16 SamplePump.setPosition! 0ml
@22:35:29.21 Exhaust.open
@22:35:30.64 <closeBypass> Bypass.close
@22:35:32.18 <FILTERING> Pressure.in :default
@22:35:32.24 Pressure.hold
@22:35:32.35 Sea 6.8m deep (24.7psia)
@22:35:32.48 SamplePump.in :prime
@22:35:32.54 SamplePump.seek 100ml,8:51
@22:37:42.42 Pressure.coast
@22:37:42.48 Bypass.open
@22:37:43.22 Intake.close
@22:37:44.10 Toroid.in :hold
@22:37:44.16 Toroid.seek :crack
@22:37:45.72 Toroid.seek :cartridge
@22:37:46.82 Toroid.in :default
@22:37:46.88 Toroid.seek :clear
@22:37:48.85 Pressurizing cartridge to 26psi above 24.8psia
@22:37:48.86 SamplePump.setPosition! 0ml
@22:37:48.90 Intake.open
@22:37:49.66 Bypass.close
@22:37:50.42 Exhaust.close
@22:37:51.21 Pressure.in :default
@22:37:51.27 Pressure.hold
@22:37:51.44 SamplePump.in :charge
@22:37:51.50 SamplePump.seek 2.3ml,42 seconds
@22:38:01.49 Pressure.coast
@22:38:01.54 Intake.close
@22:38:02.36 Pressurized cartridge with 0.444ml to 26.4psi
@22:38:02.38 Isolated @45.2psia (20.4psi)
@22:38:02.39 Verifying cartridge holds at least 0.75..0.85 of 45.2psia
@22:38:02.43 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@22:38:20.59 Toroid.in :hold
@22:38:20.65 Toroid.seek :bypass
@22:38:22.29 Toroid.in :default
@22:38:22.35 Toroid.seek :clear
@22:38:24.03 Cartridge::Sampler::Leaky in FILTERING -- Isolated cartridge pressure fell by 25% to 33.9psia
@22:38:24.04 Retry #1 of 2
@22:38:24.06 Cmd::SpareCartridge in FILTERING -- 1 spare remaining
@22:38:24.11 Selecting Cartridge 29
@22:38:24.25 Gate.power :heat,:OFF
@22:38:24.27 Gate.power :cartridge,:OFF
@22:38:24.53 <Air> Air.in :unlocking
@22:38:24.64 Air.seek :clear
@22:38:26.03 <Lysis1> Lysis1.in :unlocking
@22:38:26.15 Lysis1.seek :clear
@22:38:26.22 <Plunge> Plunge.in :unlocking
@22:38:26.29 Plunge.seek :clear
@22:38:27.47 <FILTERING> Carousel.seek 29
@22:38:28.17 Lysis1.in :fast
@22:38:28.23 Lysis1.seek :locked
@22:38:30.83 <Plunge> Plunge.in :default
@22:38:30.91 Plunge.seek :locked
@22:38:31.02 <Air> Air.in :fast
@22:38:31.08 Air.seek :locked
@22:38:33.68 <FILTERING> Powering cartridge bus
@22:38:35.80 Gate.setIR 1750,5000,0,6
@22:38:35.93 Gate.setIR 0,5000,0,6
@22:38:35.99 PuckHeater.configure :default
@22:38:36.03 Gate.power :heat,:ON,limit to 5000mA
@22:38:36.73 Mounted lysis Cartridge base#L5-0018, top#L8-0070, board#Q05043
@22:38:36.77 Using default puck heat loss curve for HeatingAccelInCarousel
@22:38:36.97 Bypass.open
@22:38:37.78 Toroid.in :hold
@22:38:37.84 Toroid.seek :crack
@22:38:39.41 Toroid.seek :cartridge
@22:38:40.51 Toroid.in :default
@22:38:40.57 Toroid.seek :clear
@22:38:42.37 Exhaust.open
@22:38:43.22 Pressurizing cartridge to 26psi above 21.5psia
@22:38:43.23 SamplePump.setPosition! 0ml
@22:38:43.27 Intake.open
@22:38:44.02 Bypass.close
@22:38:44.74 Exhaust.close
@22:38:45.52 Pressure.in :default
@22:38:45.58 Pressure.hold
@22:38:45.76 SamplePump.seek 2.3ml,42 seconds
@22:38:55.50 Pressure.coast
@22:38:55.55 Intake.close
@22:38:56.37 Pressurized cartridge with 0.422ml to 26.9psi
@22:38:56.39 Isolated @41.9psia (20.4psi)
@22:38:56.40 Verifying cartridge holds at least 0.75..0.85 of 41.9psia
@22:38:56.44 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@22:39:01.06 Sampling 1000.0ml
@22:39:01.08 SamplePump.setPosition! 0ml
@22:39:01.13 Exhaust.open
@22:39:01.81 Intake.open
@22:39:02.65 Pressure.in :default
@22:39:02.71 Pressure.hold
@22:39:02.82 Sea 4.9m deep (21.9psia)
@22:39:02.96 SamplePump.in :sample
@22:39:03.03 SamplePump.seek 1000ml,1:23:53
@22:39:05.69 <sampling> Cartridge 29 filtering 1/1000ml at 0.31ml/s with 19.7psi vacuum 3.0m deep (19.1psia)
@22:40:05.68 Cartridge 29 filtering 19/1000ml at 0.30ml/s with 21.6psi vacuum 3.8m deep (20.3psia)
@22:41:05.68 Cartridge 29 filtering 37/1000ml at 0.31ml/s with 26.4psi vacuum 3.3m deep (19.6psia)
@22:42:05.68 Cartridge 29 filtering 54/1000ml at 0.29ml/s with 22.8psi vacuum 4.1m deep (20.8psia)
@22:43:05.68 Cartridge 29 filtering 71/1000ml at 0.31ml/s with 24.6psi vacuum 4.3m deep (21.1psia)
@22:44:05.68 Cartridge 29 filtering 87/1000ml at 0.29ml/s with 26.1psi vacuum 3.0m deep (19.1psia)
@22:45:05.68 Cartridge 29 filtering 103/1000ml at 0.30ml/s with 23.4psi vacuum 4.5m deep (21.4psia)
@22:46:05.68 Cartridge 29 filtering 119/1000ml at 0.26ml/s with 20.7psi vacuum 3.7m deep (20.1psia)
@22:47:05.68 Cartridge 29 filtering 134/1000ml at 0.21ml/s with 23.1psi vacuum 3.4m deep (19.7psia)
@22:48:05.68 Cartridge 29 filtering 150/1000ml at 0.26ml/s with 27.1psi vacuum 2.4m deep (18.2psia)
@22:49:05.68 Cartridge 29 filtering 164/1000ml at 0.28ml/s with 23.3psi vacuum 4.4m deep (21.2psia)
@22:50:05.68 Cartridge 29 filtering 179/1000ml at 0.27ml/s with 21.1psi vacuum 3.1m deep (19.3psia)
@22:51:05.68 Cartridge 29 filtering 193/1000ml at 0.26ml/s with 21.3psi vacuum 5.6m deep (23.0psia)
@22:52:05.68 Cartridge 29 filtering 207/1000ml at 0.24ml/s with 25.2psi vacuum 2.5m deep (18.4psia)
@22:53:05.68 Cartridge 29 filtering 221/1000ml at 0.22ml/s with 21.8psi vacuum 5.3m deep (22.5psia)
@22:54:05.68 Cartridge 29 filtering 235/1000ml at 0.20ml/s with 26.0psi vacuum 2.3m deep (18.0psia)
@22:55:05.68 Cartridge 29 filtering 248/1000ml at 0.20ml/s with 23.3psi vacuum 2.3m deep (18.1psia)
@22:56:05.68 Cartridge 29 filtering 261/1000ml at 0.25ml/s with 25.5psi vacuum 5.1m deep (22.3psia)
@22:57:05.68 Cartridge 29 filtering 274/1000ml at 0.24ml/s with 25.4psi vacuum 3.2m deep (19.5psia)
@22:58:05.68 Cartridge 29 filtering 287/1000ml at 0.20ml/s with 24.6psi vacuum 2.6m deep (18.5psia)
@22:59:05.68 Cartridge 29 filtering 300/1000ml at 0.20ml/s with 26.6psi vacuum 4.0m deep (20.6psia)
@23:00:05.68 Cartridge 29 filtering 313/1000ml at 0.22ml/s with 21.6psi vacuum 2.6m deep (18.6psia)
@23:01:05.68 Cartridge 29 filtering 325/1000ml at 0.21ml/s with 23.7psi vacuum 3.6m deep (20.1psia)
@23:02:05.68 Cartridge 29 filtering 337/1000ml at 0.21ml/s with 26.2psi vacuum 1.6m deep (17.1psia)
@23:03:05.68 Cartridge 29 filtering 350/1000ml at 0.20ml/s with 24.6psi vacuum 5.2m deep (22.4psia)
@23:04:05.68 Cartridge 29 filtering 362/1000ml at 0.21ml/s with 24.2psi vacuum 2.7m deep (18.6psia)
@23:04:08.10 <FILTERING> Flow averaged 0.24ml/s over 25:06
@23:04:08.13 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi
@23:04:08.70 Relieved excess vacuum in .6 seconds
@23:04:08.74 SamplePump.seek 1000ml,53:40
@23:04:52.39 Flow averaged 0.20ml/s over 44 seconds
@23:04:52.42 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@23:04:52.98 Relieved excess vacuum in .6 seconds
@23:04:53.02 SamplePump.seek 1000ml,52:56
@23:04:59.22 Flow estimate reduced to 0.16ml/s
@23:04:59.25 <closeIntake> Intake.close
@23:04:59.31 <closeExhaust> Exhaust.close
@23:04:59.48 <FILTERING> Toroid.in :hold
@23:04:59.55 Toroid.seek :bypass
@23:05:01.18 Toroid.in :default
@23:05:01.24 Toroid.seek :clear
@23:05:02.85 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@23:05:02.86 Pressure.coast
@23:05:03.05 Sampled  372.4ml
@23:05:05.26 <LRAUV> -> Cmd.startProcessing
@23:05:06.69 <PROCESSING> SPR.command \"read_sequences\"
@23:05:07.10 SPR.command \"stats_on\"
@23:05:07.14 SPR.command \"start 0 1\"
@23:05:09.15 <SPRlogger> Begun log /var/log/esp/spr21apr2223h.spr
@23:05:09.95 <PROCESSING> Handoff.select :waste
@23:05:16.63 Syringe tare is 20% of full scale
@23:05:16.65 PinPort.in :locking
@23:05:16.72 PinPort.seek :locked
@23:05:17.21 <posArchive> Archive.in :fast
@23:05:17.27 <posLyS2> Lysis2.in :fast
@23:05:17.30 <PROCESSING> Lysis1.seek 1350uL
@23:05:17.42 <posArchive> Archive.seek :full
@23:05:17.47 <posLyS2> Lysis2.seek :full
@23:05:19.55 <PROCESSING> Plunge.seek :reagent
@23:05:20.45 Evacuating puck with filter bubble point of 50psi
@23:05:20.47 PinPort.seek :locked
@23:05:20.55 Handoff.select :waste
@23:05:27.14 Air syringe pumping up to 1 stroke to reach 8psi
@23:05:27.15 Air stroke #1 of 1
@23:05:27.24 Air.in :pressurize
@23:05:27.30 Air.seek :pressurized
@23:05:38.56 0.1psi after pressurize
@23:05:38.57 Air.in :compress
@23:05:38.64 Air.seek :evac
@23:05:42.74 0.4psi after compress
@23:05:55.31 0.1psi after compress (stable)
@23:05:55.32 Air.in :default
@23:05:55.40 Air.hold
@23:05:55.56 at 0.1psi Opened to waste
@23:05:55.58 Handoff.select :blocked
@23:05:55.71 Archive syringe pumping up to 5 strokes to reach 50psi
@23:05:55.72 Archive stroke #1 of 5
@23:05:55.81 Archive.in :pressurize
@23:05:55.88 Archive.seek :pressurized
@23:06:08.62 9.5psi after pressurize
@23:06:08.63 Archive.in :compress
@23:06:08.70 Archive.seek :empty
@23:06:24.60 35.4psi after compress
@23:06:30.26 Reached 50.1 of 50.0psi after pumping 1730uL (1.00 strokes)
@23:06:30.27 Archive.in :default
@23:06:30.34 Archive.hold
@23:06:30.43 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position
@23:06:30.45 Handoff.select :waste
@23:06:41.13 delay 1:00
@23:07:41.15 Handoff.select :blocked
@23:08:03.82 Handoff.select :waste
@23:08:03.94 0.3psi at evacEnd
@23:08:03.95 Handoff.select :blocked
@23:08:04.07 Archive.in :fast
@23:08:04.14 Archive.seek :topVent
@23:08:17.86 Stable @1.1psi after Handoff blocked
@23:08:17.87 Archive syringe pumping up to 5 strokes to reach 31psi
@23:08:17.89 Archive stroke #1 of 5
@23:08:17.98 Archive.in :pressurize
@23:08:18.04 Archive.seek :pressurized
@23:08:30.78 1.1psi after pressurize
@23:08:30.79 Archive.in :compress
@23:08:30.87 Archive.seek :empty
@23:08:46.77 1.1psi after compress
@23:08:59.37 1.1psi after compress (stable)
@23:08:59.39 Archive.in :gulp
@23:08:59.46 Archive.seek :topVent
@23:09:06.67 1.7psi after gulp
@23:09:19.24 1.8psi after gulp (stable)
@23:09:19.25 Archive stroke #2 of 5
@23:09:19.34 Archive.in :pressurize
@23:09:19.40 Archive.seek :pressurized
@23:09:32.14 1.9psi after pressurize
@23:09:32.16 Archive.in :compress
@23:09:32.22 Archive.seek :empty
@23:09:48.13 2.4psi after compress
@23:11:06.20 23.6psi after compress (stable)
@23:11:06.22 Archive.in :gulp
@23:11:06.29 Archive.seek :topVent
@23:11:13.49 26.3psi after gulp
@23:11:26.06 26.9psi after gulp (stable)
@23:11:26.07 Archive stroke #3 of 5
@23:11:26.16 Archive.in :pressurize
@23:11:26.22 Archive.seek :pressurized
@23:11:38.96 27.0psi after pressurize
@23:11:38.97 Archive.in :compress
@23:11:39.05 Archive.seek :empty
@23:11:54.93 27.2psi after compress
@23:12:01.38 Reached 31.1 of 31.1psi after pumping 5190uL (3.00 strokes)
@23:12:01.39 Archive.in :default
@23:12:01.45 Archive.hold
@23:12:01.52 Handoff.select :waste
@23:12:19.71 delay 1:00
@23:13:19.73 Handoff.select :blocked
@23:13:58.42 Handoff.select :waste
@23:13:58.54 7.5psi at evacEnd
@23:13:58.55 Air.seek :fullVent
@23:14:00.01 Archive.in :fast
@23:14:00.08 Archive.seek :topVent
@23:14:07.23 Seawater evacuated, Presure above/below filter released. Lysis begins
@23:14:07.32 Lysis1.in :slow
@23:14:07.38 Lysis1.seek 1050uL
@23:14:37.42 delay 5 seconds
@23:14:42.44 Handoff.select :blocked
@23:14:42.60 Lysis1.seek 450uL
@23:15:42.59 delay 3 seconds
@23:15:45.67 Air.in to mix into 1.37806 parts Lysis1
@23:15:46.22 <Airmixer> Air.seek :evac
@23:15:46.28 <PROCESSING> Lysis1.seek 300uL
@23:16:01.36 Lysis1.seek :empty
@23:16:31.47 Lysis buffer delivered @20.2psi
@23:16:31.48 Polling Can every 2:00
@23:16:32.03 PuckHeater.configure :default #ambient 11.6C
@23:16:32.08 PuckHeater.seek 80.2C
@23:17:42.61 PuckHeater.hold 2:00,80.2C
@23:19:42.68 Polling Can every 2:00
@23:19:43.23 PuckHeater.configure :default #ambient 11.7C
@23:19:43.27 PuckHeater.hold 30 seconds,79.2C
@23:20:13.46 Polling Can every 2:00
@23:20:14.02 PuckHeater.configure :default #ambient 11.6C
@23:20:14.06 PuckHeater.hold 30 seconds,78.6C
@23:20:44.25 Polling Can every 2:00
@23:20:44.80 PuckHeater.configure :default #ambient 11.6C
@23:20:44.85 PuckHeater.hold 10:00,78.1C
@23:30:45.09 Lysis complete. Syringe pressure @26.3psi
@23:30:45.10 Handoff.select :blocked
@23:30:45.14 Archive syringe pumping up to 3 strokes to reach 40psi
@23:30:45.15 Archive stroke #1 of 3
@23:30:45.24 Archive.in :pressurize
@23:30:45.30 Archive.seek :pressurized
@23:30:58.04 25.8psi after pressurize
@23:30:58.05 Archive.in :compress
@23:30:58.13 Archive.seek :empty
@23:31:06.36 Reached 40.5 of 40.0psi after pumping 1566uL (0.91 strokes)
@23:31:06.38 Archive.in :default
@23:31:06.45 Archive.hold
@23:31:06.53 delay 2 seconds
@23:31:08.56 Archive.in :fast
@23:31:08.67 Archive.seek :full
@23:31:15.30 Lysis1.seek 138uL
@23:31:29.18 delay 1 second
@23:31:30.21 <fillArchive> Archive.seek :full
@23:31:30.23 <PROCESSING> Lysis1.seek :empty
@23:31:44.22 Initial air pressure @47.2psi
@23:31:44.29 Lysis1.in :extract
@23:31:44.37 Lysis1.seek 600uL,nil
@23:31:46.05 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@23:31:46.07 Archive stroke #1 of 6
@23:31:46.20 Archive.in :pressurize
@23:31:46.27 Archive.seek :pressurized
@23:31:59.00 16.0psi after pressurize
@23:31:59.01 Archive.in :compress
@23:31:59.08 Archive.seek :empty
@23:32:14.97 15.2psi after compress
@23:32:27.54 15.8psi after compress (stable)
@23:32:32.71 Recovering 5.2uL/s
@23:32:34.23 Archive.in :default
@23:32:34.28 Archive.hold
@23:32:34.42 <PROCESSING> Initial 600uL lysate @15.6psi
@23:32:34.43 Handoff.select :blocked
@23:32:34.52 Initial air pressure @15.8psi
@23:32:34.60 Lysis1.seek 825uL,nil
@23:32:34.68 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@23:32:34.69 Archive stroke #1 of 6
@23:32:34.83 Archive.in :compress
@23:32:34.90 Archive.seek :empty
@23:32:35.07 16.3psi after compress
@23:32:47.65 15.8psi after compress (stable)
@23:32:52.82 Recovering 2.7uL/s
@23:32:57.89 Recovering 2.6uL/s
@23:33:02.96 Archive.in :gulp
@23:33:03.02 Archive.seek :topVent
@23:33:10.25 16.1psi after gulp
@23:33:22.83 15.8psi after gulp (stable)
@23:33:22.84 Archive stroke #2 of 6
@23:33:22.93 Archive.in :pressurize
@23:33:23.01 Archive.seek :pressurized
@23:33:35.74 15.8psi after pressurize
@23:33:35.77 Archive.in :compress
@23:33:35.84 Archive.seek :empty
@23:33:51.73 15.4psi after compress
@23:33:52.68 Archive.in :default
@23:33:52.73 Archive.hold
@23:33:52.93 <PROCESSING> Recovered 825uL lysate @16.5psi
@23:33:52.94 Air.in :halfSpeed
@23:33:53.02 Air.seek :fullVent
@23:33:55.26 Puck Top Vented, bottom @2.8psi
@23:33:55.27 Handoff.select :waste
@23:33:55.38 delay 5 seconds
@23:34:00.47 Handoff open to waste, bottom @-0.0psi
@23:34:00.48 Air.in :default
@23:34:00.55 Air.seek :evac
@23:34:01.98 Lysis2.in :DAmix
@23:34:02.08 Lysis1.in :DAmix
@23:34:02.63 <Lysis2mixer> Lysis2.seek 1480uL
@23:34:02.66 <PROCESSING> Lysis1.seek 575uL
@23:34:37.75 delay 5 seconds
@23:34:42.78 Handoff.select SPR
@23:34:42.89 SPR.command \"start 2 14\"
@23:34:44.43 delay 10 seconds
@23:34:54.45 SPR.command \"start 1 0\"
@23:34:57.69 Waiting up to 4:00 for SPR to enter state 1
@23:37:00.06 SPR.command \"hold_on\"
@23:37:01.47 SPR Dilution 1 of 1 -- Running Experiment 1
@23:37:01.91 <Lysis2mixer> Lysis2.seek 1180uL
@23:37:01.96 <PROCESSING> Lysis1.seek 275uL
@23:37:43.96 volL1 = 300.0 and volL2 = 300.0
@23:37:43.97 delay 15 seconds
@23:37:59.00 SPR.command \"hold_off\"
@23:38:02.12 Waiting up to 38:00 for SPR to enter state 7
@00:04:45.67PDT23-Apr-21 SPR Analysis has Completed
@00:04:45.69 Handoff.select :waste
@00:04:46.60 <SPRlogger> Ended log /var/log/esp/spr21apr2223h.spr
@00:04:46.61 <PROCESSING> SPR.command \"stats_off\"
@00:04:48.10 <LRAUV> -> Cmd.stop
@00:04:48.19 <closeHandoff> Handoff.select :close
@00:04:48.31 <LRAUV> Gate.power :heat,:OFF
@00:04:48.33 Gate.power :cartridge,:OFF
@00:04:48.41 Carousel.seek 29
@00:04:48.58 Plunge.seek :locked
@00:04:49.47 PinPort.in :unlocking
@00:04:49.53 PinPort.seek :clear
@00:04:50.07 <Archive> Archive.in :unlocking
@00:04:50.20 Archive.seek :clear
@00:04:50.28 <Air> Air.in :unlocking
@00:04:50.39 Air.seek :clear
@00:04:50.46 <Lysis1> Lysis1.in :unlocking
@00:04:50.58 Lysis1.seek :clear
@00:04:50.64 <Lysis2> Lysis2.in :unlocking
@00:04:50.72 Lysis2.seek :clear
@00:04:54.18 Slide::Error in LRAUV -- Archive Syringe (ArS) positionErr at 955uL (sensed 929uL)
@00:04:54.20 <LRAUV> Archive.coast
@00:04:54.25 Air.coast
@00:04:54.30 Lysis1.coast
@00:04:54.35 Lysis2.coast
@00:04:54.40 Plunge.coast
@00:04:54.45 SamplePump.coast
@00:04:54.50 Toroid.coast
@00:04:54.56 Carousel.coast
@00:04:54.61 PinPort.coast
@00:04:54.79 Gate.power :motor,:OFF
@00:04:54.81 Gate.power :core,:OFF
@00:04:54.83 Gate.power :analytic1,:OFF
@00:04:54.85 Gate.power :analytic2,:OFF
@00:04:54.87 Gate.power :analytic3,:OFF
@00:04:54.89 Gate.power :main,:OFF
@00:04:54.91 ready to power off
##-- file closed 2021-04-23T09:11:50.634Z

##-- file opened 2021-04-23T09:11:50.636Z
@02:11:51.43 -> showStatus
@02:11:53.45 -> Cmd.loadCartridge -6
@02:11:53.59 Gate.power :main,:ON,limit to 5000mA
@02:11:53.60 Gate.power :analytic1,:ON,limit to 5000mA
@02:11:53.62 Gate.power :analytic2,:ON,limit to 5000mA
@02:11:53.64 Gate.power :analytic3,:ON,limit to 5000mA
@02:11:53.78 Powering core bus
@02:11:56.05 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@02:11:56.18 <LRAUV> Archive.configure :default
@02:11:56.30 Air.configure :default
@02:11:56.40 Lysis1.configure :default
@02:11:56.50 Lysis2.configure :default
@02:11:56.62 Plunge.configure :default
@02:11:56.75 SamplePump.configure :default
@02:11:56.90 ExtraPump.configure :default
@02:11:57.00 Toroid.configure :default
@02:11:57.13 Carousel.configure :default
@02:11:57.26 Pressure.configure :default
@02:11:57.37 PinPort.configure :default
@02:11:57.43 All elf controllers running firmware version 4.741
@02:11:57.46 Gate.power :motor,:ON,limit to 5000mA
@02:11:57.62 <Archive_home> Archive.in :default
@02:11:57.95 Archive.home.jog 0
@02:11:58.46 <Toroid_home> Toroid.in :default
@02:11:58.54 <Plunge_home> Plunge.in :default
@02:11:58.57 <Lysis1_home> Lysis1.in :default
@02:11:58.62 <PinPort_home> PinPort.in :default
@02:11:58.68 <Lysis2_home> Lysis2.in :default
@02:11:58.72 <Air_home> Air.in :default
@02:11:58.89 <Toroid_home> Toroid.home.jog 0
@02:11:58.92 <Plunge_home> Plunge.home.jog 0
@02:11:58.96 <PinPort_home> PinPort.home.jog 0
@02:11:59.00 <Lysis1_home> Lysis1.home.jog 0
@02:11:59.03 <Lysis2_home> Lysis2.home.jog 0
@02:11:59.09 <Air_home> Air.home.jog 0
@02:11:59.42 <Archive_home> Archive.in :homing
@02:11:59.63 <Toroid_home> Toroid.in :homing
@02:11:59.66 <Plunge_home> Plunge.in :homing
@02:11:59.71 <Archive_home> Archive.jog 4565
@02:11:59.75 <Lysis1_home> Lysis1.in :homing
@02:11:59.79 <PinPort_home> PinPort.in :homing
@02:11:59.97 <Toroid_home> Toroid.jog 600
@02:12:00.03 <Plunge_home> Plunge.jog 1577
@02:12:00.07 <PinPort_home> PinPort.jog -108
@02:12:00.11 <Lysis1_home> Lysis1.jog 1419
@02:12:00.27 <Air_home> Air.in :homing
@02:12:00.29 <Lysis2_home> Lysis2.in :homing
@02:12:00.39 Lysis2.jog 1429
@02:12:00.41 <Air_home> Air.jog 170
@02:12:00.51 <PinPort_home> PinPort.setRawPosition! 0
@02:12:00.56 PinPort.seek :home
@02:12:00.64 PinPort.in :default
@02:12:00.89 <Air_home> Air.setRawPosition! 50
@02:12:00.94 Air.seek :home
@02:12:01.21 Air.in :default
@02:12:01.31 <Lysis1_home> Lysis1.setRawPosition! 50
@02:12:01.36 Lysis1.seek :home
@02:12:01.44 <Plunge_home> Plunge.setRawPosition! 50
@02:12:01.49 Plunge.seek :home
@02:12:01.58 <Lysis2_home> Lysis2.setRawPosition! 50
@02:12:01.64 Lysis2.seek :home
@02:12:01.72 <Lysis1_home> Lysis1.in :default
@02:12:01.81 <Plunge_home> Plunge.in :default
@02:12:01.92 <Lysis2_home> Lysis2.in :default
@02:12:02.08 <Toroid_home> Toroid.setRawPosition! 0
@02:12:02.12 Toroid.seek :home
@02:12:02.22 Toroid.in :default
@02:12:02.35 <Archive_home> Archive.setRawPosition! 50
@02:12:02.39 Archive.seek :home
@02:12:02.67 Archive.in :default
@02:12:03.05 <LRAUV> Toroid.seek :clear
@02:12:04.66 Carousel.in :default
@02:12:04.72 Carousel.home.jog 0
@02:12:05.20 Carousel.in :default
@02:12:05.26 Carousel.seek 29
@02:12:05.40 Selecting Cartridge 32
@02:12:05.63 Carousel.seek 32
@02:12:06.52 Lysis1.in :fast
@02:12:06.58 Lysis1.seek :locked
@02:12:09.18 <Plunge> Plunge.seek :locked
@02:12:09.30 <Air> Air.in :fast
@02:12:09.36 Air.seek :locked
@02:12:11.96 <LRAUV> Powering cartridge bus
@02:12:14.08 Gate.setIR 1750,5000,0,6
@02:12:14.21 Gate.setIR 0,5000,0,6
@02:12:14.26 Gate.power :cartridge,:OFF
@02:12:14.79 Mounted archive Cartridge
@02:12:33.05 -> Cmd.startFiltering
@02:12:33.50 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@02:12:33.51 SamplePump.setPosition! 0ml
@02:12:33.56 Bypass.open
@02:12:35.05 Intake.open
@02:12:35.84 Sea 5.4m deep (22.6psia)
@02:12:35.85 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@02:12:38.43 Vacuum tare is 51% of full scale
@02:12:38.55 Pressure.in :default
@02:12:38.61 Pressure.hold
@02:12:38.78 SamplePump.in :prime
@02:12:38.85 SamplePump.seek 10ml,1:21
@02:12:48.24 Pressure.coast
@02:12:48.29 SamplePump.setPosition! 0ml
@02:12:48.34 Exhaust.open
@02:12:49.74 <closeBypass> Bypass.close
@02:12:51.28 <FILTERING> Pressure.in :default
@02:12:51.34 Pressure.hold
@02:12:51.44 Sea 4.9m deep (21.9psia)
@02:12:51.57 SamplePump.in :prime
@02:12:51.64 SamplePump.seek 100ml,8:51
@02:15:01.62 Pressure.coast
@02:15:01.68 Bypass.open
@02:15:02.39 Intake.close
@02:15:03.24 Toroid.in :hold
@02:15:03.30 Toroid.seek :crack
@02:15:04.86 Toroid.seek :cartridge
@02:15:05.95 Toroid.in :default
@02:15:06.01 Toroid.seek :clear
@02:15:07.97 Pressurizing cartridge to 26psi above 23.6psia
@02:15:07.98 SamplePump.setPosition! 0ml
@02:15:08.02 Intake.open
@02:15:08.78 Bypass.close
@02:15:09.54 Exhaust.close
@02:15:10.32 Pressure.in :default
@02:15:10.38 Pressure.hold
@02:15:10.55 SamplePump.in :charge
@02:15:10.61 SamplePump.seek 2.3ml,42 seconds
@02:15:16.28 Pressure.coast
@02:15:16.33 Intake.close
@02:15:17.20 Pressurized cartridge with 0.141ml to 26.4psi
@02:15:17.21 Isolated @43.8psia (20.2psi)
@02:15:17.22 Verifying cartridge holds at least 0.75..0.85 of 43.8psia
@02:15:17.25 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@02:15:21.87 Sampling 1000.0ml
@02:15:21.89 SamplePump.setPosition! 0ml
@02:15:21.94 Exhaust.open
@02:15:22.64 Intake.open
@02:15:23.52 Pressure.in :default
@02:15:23.58 Pressure.hold
@02:15:23.69 Sea 5.6m deep (22.9psia)
@02:15:23.82 SamplePump.in :sample
@02:15:23.89 SamplePump.seek 1000ml,1:23:53
@02:15:26.68 <sampling> Cartridge 32 filtering 1/1000ml at 0.27ml/s with 24.5psi vacuum 1.9m deep (17.5psia)
@02:16:26.56 Cartridge 32 filtering 19/1000ml at 0.32ml/s with 22.9psi vacuum
@02:17:26.56 Cartridge 32 filtering 38/1000ml at 0.31ml/s with 21.6psi vacuum 5.7m deep (23.1psia)
@02:18:26.56 Cartridge 32 filtering 55/1000ml at 0.30ml/s with 23.0psi vacuum 2.2m deep (18.0psia)
@02:19:26.56 Cartridge 32 filtering 72/1000ml at 0.29ml/s with 28.6psi vacuum 4.6m deep (21.5psia)
@02:20:26.56 Cartridge 32 filtering 89/1000ml at 0.24ml/s with 22.6psi vacuum
@02:21:26.56 Cartridge 32 filtering 106/1000ml at 0.22ml/s with 23.7psi vacuum 3.6m deep (20.1psia)
@02:22:26.56 Cartridge 32 filtering 122/1000ml at 0.28ml/s with 25.5psi vacuum 4.7m deep (21.7psia)
@02:23:26.56 Cartridge 32 filtering 137/1000ml at 0.28ml/s with 24.0psi vacuum
@02:24:26.56 Cartridge 32 filtering 153/1000ml at 0.22ml/s with 21.5psi vacuum 4.0m deep (20.7psia)
@02:25:26.56 Cartridge 32 filtering 168/1000ml at 0.21ml/s with 22.8psi vacuum 1.9m deep (17.5psia)
@02:26:26.56 Cartridge 32 filtering 183/1000ml at 0.24ml/s with 27.5psi vacuum 4.5m deep (21.3psia)
@02:27:26.56 Cartridge 32 filtering 197/1000ml at 0.20ml/s with 26.1psi vacuum
@02:28:26.56 Cartridge 32 filtering 212/1000ml at 0.21ml/s with 21.9psi vacuum 3.9m deep (20.4psia)
@02:29:26.56 Cartridge 32 filtering 226/1000ml at 0.27ml/s with 22.5psi vacuum 5.1m deep (22.3psia)
@02:30:26.56 Cartridge 32 filtering 240/1000ml at 0.24ml/s with 24.7psi vacuum 2.8m deep (18.9psia)
@02:31:26.56 Cartridge 32 filtering 253/1000ml at 0.25ml/s with 22.1psi vacuum 6.4m deep (24.1psia)
@02:32:26.56 Cartridge 32 filtering 267/1000ml at 0.22ml/s with 23.6psi vacuum 2.9m deep (19.0psia)
@02:33:26.56 Cartridge 32 filtering 280/1000ml at 0.22ml/s with 23.4psi vacuum 3.0m deep (19.2psia)
@02:34:26.56 Cartridge 32 filtering 294/1000ml at 0.21ml/s with 25.2psi vacuum 4.1m deep (20.8psia)
@02:35:26.56 Cartridge 32 filtering 307/1000ml at 0.23ml/s with 27.7psi vacuum 2.0m deep (17.6psia)
@02:36:26.56 Cartridge 32 filtering 320/1000ml at 0.20ml/s with 22.8psi vacuum 3.8m deep (20.3psia)
@02:37:26.56 Cartridge 32 filtering 332/1000ml at 0.20ml/s with 25.5psi vacuum
@02:38:26.56 Cartridge 32 filtering 345/1000ml at 0.23ml/s with 24.5psi vacuum 5.3m deep (22.5psia)
@02:39:26.56 Cartridge 32 filtering 358/1000ml at 0.20ml/s with 26.4psi vacuum 3.4m deep (19.7psia)
@02:40:26.56 Cartridge 32 filtering 370/1000ml at 0.22ml/s with 25.1psi vacuum 4.6m deep (21.5psia)
@02:41:26.56 Cartridge 32 filtering 382/1000ml at 0.20ml/s with 24.9psi vacuum 2.8m deep (18.8psia)
@02:42:26.56 Cartridge 32 filtering 395/1000ml at 0.21ml/s with 25.4psi vacuum 2.9m deep (18.9psia)
@02:43:26.56 Cartridge 32 filtering 407/1000ml at 0.20ml/s with 26.7psi vacuum 5.4m deep (22.7psia)
@02:43:29.97 <FILTERING> Flow averaged 0.24ml/s over 28:07
@02:43:30.00 Waiting up to 8 seconds for vacuum to reduce from 26.0 to 18.0psi
@02:43:30.58 Relieved excess vacuum in .6 seconds
@02:43:30.60 SamplePump.seek 1000ml,49:55
@02:43:35.27 Flow estimate reduced to 0.19ml/s
@02:43:35.30 <closeIntake> Intake.close
@02:43:35.35 <closeExhaust> Exhaust.close
@02:43:35.54 <FILTERING> Toroid.in :hold
@02:43:35.60 Toroid.seek :bypass
@02:43:37.23 Toroid.in :default
@02:43:37.29 Toroid.seek :clear
@02:43:38.90 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@02:43:38.91 Pressure.coast
@02:43:39.09 Sampled  408.4ml
@02:43:41.23 <LRAUV> -> Cmd.startProcessing
@02:43:41.59 <PROCESSING> Plunge.seek :reagent
@02:43:42.43 Sample loop closed. Ready to Deliver Reagents
@02:43:42.44 Archive.seek :sealed
@02:43:46.71 Archive.seek :evac
@02:44:00.65 delay 1:00
@02:45:00.68 Archive.seek :clear
@02:45:18.39 RNAlater soak for 10:00
@02:55:18.42 Archive.seek :tVent
@02:55:21.00 delay 1 second
@02:55:22.02 Archive.seek :evac
@02:55:37.64 delay 10 seconds
@02:55:47.67 Sample preserved and evacuated
@02:55:48.86 <LRAUV> -> Cmd.stop
@02:55:49.00 Carousel.seek 32
@02:55:49.17 Plunge.seek :locked
@02:55:50.11 <Archive> Archive.in :unlocking
@02:55:50.23 Archive.seek :clear
@02:55:50.30 <Air> Air.in :unlocking
@02:55:50.43 Air.seek :clear
@02:55:50.51 <Lysis1> Lysis1.in :unlocking
@02:55:50.65 Lysis1.seek :clear
@02:55:54.27 <LRAUV> Archive.coast
@02:55:54.33 Air.coast
@02:55:54.37 Lysis1.coast
@02:55:54.42 Lysis2.coast
@02:55:54.47 Plunge.coast
@02:55:54.52 SamplePump.coast
@02:55:54.57 Toroid.coast
@02:55:54.62 Carousel.coast
@02:55:54.67 PinPort.coast
@02:55:54.85 Gate.power :motor,:OFF
@02:55:54.87 Gate.power :core,:OFF
@02:55:54.89 Gate.power :analytic1,:OFF
@02:55:54.91 Gate.power :analytic2,:OFF
@02:55:54.93 Gate.power :analytic3,:OFF
@02:55:54.95 Gate.power :main,:OFF
@02:55:54.97 Safely stopped and ready to power off
##-- file closed 2021-04-23T10:10:19.490Z

##-- file opened 2021-04-23T10:10:19.492Z
@03:10:20.11 -> showStatus
@03:10:22.13 -> Cmd.loadCartridge -9
@03:10:22.27 Gate.power :main,:ON,limit to 5000mA
@03:10:22.29 Gate.power :analytic1,:ON,limit to 5000mA
@03:10:22.31 Gate.power :analytic2,:ON,limit to 5000mA
@03:10:22.34 Gate.power :analytic3,:ON,limit to 5000mA
@03:10:22.47 Powering core bus
@03:10:24.74 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@03:10:24.87 <LRAUV> Archive.configure :default
@03:10:24.99 Air.configure :default
@03:10:25.09 Lysis1.configure :default
@03:10:25.19 Lysis2.configure :default
@03:10:25.32 Plunge.configure :default
@03:10:25.44 SamplePump.configure :default
@03:10:25.59 ExtraPump.configure :default
@03:10:25.69 Toroid.configure :default
@03:10:25.82 Carousel.configure :default
@03:10:25.94 Pressure.configure :default
@03:10:26.05 PinPort.configure :default
@03:10:26.12 All elf controllers running firmware version 4.741
@03:10:26.15 Gate.power :motor,:ON,limit to 5000mA
@03:10:26.50 <Archive_home> Archive.in :default
@03:10:27.07 <Toroid_home> Toroid.in :default
@03:10:27.15 <Plunge_home> Plunge.in :default
@03:10:27.18 <Lysis1_home> Lysis1.in :default
@03:10:27.22 <Archive_home> Archive.home.jog 0
@03:10:27.26 <PinPort_home> PinPort.in :default
@03:10:27.28 <Lysis2_home> Lysis2.in :default
@03:10:27.31 <Air_home> Air.in :default
@03:10:27.52 <Toroid_home> Toroid.home.jog 0
@03:10:27.56 <Plunge_home> Plunge.home.jog 0
@03:10:27.59 <Lysis2_home> Lysis2.home.jog 0
@03:10:27.61 <Lysis1_home> Lysis1.home.jog 0
@03:10:27.64 <PinPort_home> PinPort.home.jog 0
@03:10:27.67 <Air_home> Air.home.jog 0
@03:10:28.26 <Toroid_home> Toroid.in :homing
@03:10:28.30 <Plunge_home> Plunge.in :homing
@03:10:28.35 <PinPort_home> PinPort.in :homing
@03:10:28.41 <Lysis1_home> Lysis1.in :homing
@03:10:28.44 <Archive_home> Archive.in :homing
@03:10:28.48 <Lysis2_home> Lysis2.in :homing
@03:10:28.64 <Plunge_home> Plunge.jog 1580
@03:10:28.68 <Toroid_home> Toroid.jog 597
@03:10:28.72 <PinPort_home> PinPort.jog -107
@03:10:28.80 <Lysis2_home> Lysis2.jog 1430
@03:10:28.84 <Lysis1_home> Lysis1.jog 1416
@03:10:28.86 <Archive_home> Archive.jog 155
@03:10:29.04 <Air_home> Air.in :homing
@03:10:29.12 <PinPort_home> PinPort.setRawPosition! 0
@03:10:29.14 <Air_home> Air.jog 155
@03:10:29.23 <PinPort_home> PinPort.seek :home
@03:10:29.31 PinPort.in :default
@03:10:29.39 <Archive_home> Archive.setRawPosition! 50
@03:10:29.44 Archive.seek :home
@03:10:29.59 <Air_home> Air.setRawPosition! 50
@03:10:29.64 Air.seek :home
@03:10:29.74 <Archive_home> Archive.in :default
@03:10:29.91 <Air_home> Air.in :default
@03:10:29.99 <Plunge_home> Plunge.setRawPosition! 50
@03:10:30.04 Plunge.seek :home
@03:10:30.13 <Lysis2_home> Lysis2.setRawPosition! 50
@03:10:30.15 <Lysis1_home> Lysis1.setRawPosition! 50
@03:10:30.22 <Lysis2_home> Lysis2.seek :home
@03:10:30.32 <Lysis1_home> Lysis1.seek :home
@03:10:30.40 <Plunge_home> Plunge.in :default
@03:10:30.53 <Lysis2_home> Lysis2.in :default
@03:10:30.61 <Lysis1_home> Lysis1.in :default
@03:10:30.80 <Toroid_home> Toroid.setRawPosition! 0
@03:10:30.85 Toroid.seek :home
@03:10:30.94 Toroid.in :default
@03:10:31.08 <LRAUV> Toroid.seek :clear
@03:10:32.70 Carousel.in :default
@03:10:32.76 Carousel.home.jog 0
@03:10:33.25 Carousel.in :default
@03:10:33.31 Carousel.seek 32
@03:10:33.46 Selecting Cartridge 27
@03:10:33.71 Carousel.seek 27
@03:10:35.01 Lysis1.in :fast
@03:10:35.08 Lysis1.seek :locked
@03:10:37.67 <Plunge> Plunge.seek :locked
@03:10:37.79 <Air> Air.in :fast
@03:10:37.86 Air.seek :locked
@03:10:40.45 <LRAUV> Powering cartridge bus
@03:10:42.58 Gate.setIR 1750,5000,0,6
@03:10:42.71 Gate.setIR 0,5000,0,6
@03:10:42.77 PuckHeater.configure :default
@03:10:42.81 Gate.power :heat,:ON,limit to 5000mA
@03:10:43.50 Mounted lysis Cartridge board#Q05034
@03:10:43.52 Using default puck heat loss curve for HeatingAccelInCarousel
@03:10:43.54 Ignoring this cartridge's heat loss curve
@03:11:02.94 -> Cmd.startFiltering
@03:11:03.38 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@03:11:03.40 SamplePump.setPosition! 0ml
@03:11:03.44 Bypass.open
@03:11:04.93 Intake.open
@03:11:05.73 Sea 4.6m deep (21.5psia)
@03:11:05.74 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@03:11:08.32 Vacuum tare is 51% of full scale
@03:11:08.45 Pressure.in :default
@03:11:08.50 Pressure.hold
@03:11:08.67 SamplePump.in :prime
@03:11:08.80 SamplePump.seek 10ml,1:21
@03:11:18.18 Pressure.coast
@03:11:18.23 SamplePump.setPosition! 0ml
@03:11:18.28 Exhaust.open
@03:11:19.71 <closeBypass> Bypass.close
@03:11:21.28 <FILTERING> Pressure.in :default
@03:11:21.34 Pressure.hold
@03:11:21.44 Sea 4.0m deep (20.5psia)
@03:11:21.57 SamplePump.in :prime
@03:11:21.64 SamplePump.seek 100ml,8:51
@03:13:31.59 Pressure.coast
@03:13:31.65 Bypass.open
@03:13:32.40 Intake.close
@03:13:33.25 Toroid.in :hold
@03:13:33.31 Toroid.seek :crack
@03:13:34.86 Toroid.seek :cartridge
@03:13:35.96 Toroid.in :default
@03:13:36.02 Toroid.seek :clear
@03:13:37.99 Pressurizing cartridge to 26psi above 20.7psia
@03:13:38.00 SamplePump.setPosition! 0ml
@03:13:38.04 Intake.open
@03:13:38.78 Bypass.close
@03:13:39.54 Exhaust.close
@03:13:40.32 Pressure.in :default
@03:13:40.38 Pressure.hold
@03:13:40.55 SamplePump.in :charge
@03:13:40.62 SamplePump.seek 2.3ml,42 seconds
@03:13:53.00 Pressure.coast
@03:13:53.05 Intake.close
@03:13:53.90 Pressurized cartridge with 0.685ml to 26.5psi
@03:13:53.91 Isolated @40.8psia (20.2psi)
@03:13:53.92 Verifying cartridge holds at least 0.75..0.85 of 40.8psia
@03:13:53.96 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@03:14:01.04 Cartridge pressure fell 6% to 38.5psia
@03:14:01.12 Sampling 1000.0ml
@03:14:01.13 SamplePump.setPosition! 0ml
@03:14:01.18 Exhaust.open
@03:14:01.90 Intake.open
@03:14:03.18 Pressure.in :default
@03:14:03.24 Pressure.hold
@03:14:03.35 Sea 4.5m deep (21.3psia)
@03:14:03.51 SamplePump.in :sample
@03:14:03.62 SamplePump.seek 1000ml,1:23:53
@03:14:05.79 <sampling> Cartridge 27 filtering 1/1000ml at 0.33ml/s with 21.1psi vacuum 3.3m deep (19.5psia)
@03:15:05.79 Cartridge 27 filtering 20/1000ml at 0.29ml/s with 28.8psi vacuum 3.0m deep (19.1psia)
@03:16:05.80 Cartridge 27 filtering 39/1000ml at 0.28ml/s with 28.7psi vacuum
@03:17:05.79 Cartridge 27 filtering 57/1000ml at 0.25ml/s with 23.5psi vacuum 3.2m deep (19.4psia)
@03:18:05.80 Cartridge 27 filtering 75/1000ml at 0.31ml/s with 25.5psi vacuum 5.0m deep (22.0psia)
@03:19:05.79 Cartridge 27 filtering 92/1000ml at 0.26ml/s with 19.0psi vacuum 3.7m deep (20.2psia)
@03:20:05.80 Cartridge 27 filtering 109/1000ml at 0.27ml/s with 19.3psi vacuum 5.4m deep (22.7psia)
@03:21:05.79 Cartridge 27 filtering 126/1000ml at 0.25ml/s with 20.3psi vacuum 1.6m deep (17.0psia)
@03:22:05.80 Cartridge 27 filtering 142/1000ml at 0.26ml/s with 28.0psi vacuum 4.3m deep (21.1psia)
@03:23:05.79 Cartridge 27 filtering 158/1000ml at 0.29ml/s with 22.0psi vacuum 3.0m deep (19.2psia)
@03:24:05.80 Cartridge 27 filtering 174/1000ml at 0.24ml/s with 19.7psi vacuum 4.6m deep (21.6psia)
@03:25:05.79 Cartridge 27 filtering 190/1000ml at 0.22ml/s with 22.6psi vacuum 2.9m deep (19.0psia)
@03:26:05.80 Cartridge 27 filtering 205/1000ml at 0.21ml/s with 24.3psi vacuum 2.8m deep (18.8psia)
@03:27:05.79 Cartridge 27 filtering 220/1000ml at 0.21ml/s with 21.1psi vacuum 3.9m deep (20.5psia)
@03:28:05.80 Cartridge 27 filtering 234/1000ml at 0.25ml/s with 22.4psi vacuum 3.4m deep (19.7psia)
@03:29:05.79 Cartridge 27 filtering 249/1000ml at 0.23ml/s with 25.7psi vacuum 5.3m deep (22.5psia)
@03:30:05.80 Cartridge 27 filtering 263/1000ml at 0.27ml/s with 24.1psi vacuum 3.0m deep (19.1psia)
@03:31:05.79 Cartridge 27 filtering 277/1000ml at 0.26ml/s with 23.5psi vacuum 3.7m deep (20.2psia)
@03:32:05.80 Cartridge 27 filtering 291/1000ml at 0.23ml/s with 23.1psi vacuum 4.7m deep (21.7psia)
@03:33:05.79 Cartridge 27 filtering 305/1000ml at 0.21ml/s with 21.4psi vacuum 2.1m deep (17.8psia)
@03:34:05.80 Cartridge 27 filtering 318/1000ml at 0.23ml/s with 24.8psi vacuum 5.3m deep (22.5psia)
@03:35:05.79 Cartridge 27 filtering 331/1000ml at 0.20ml/s with 25.2psi vacuum 2.1m deep (17.8psia)
@03:36:05.80 Cartridge 27 filtering 344/1000ml at 0.21ml/s with 21.3psi vacuum 5.7m deep (23.1psia)
@03:37:05.79 Cartridge 27 filtering 357/1000ml at 0.20ml/s with 24.2psi vacuum 4.7m deep (21.7psia)
@03:38:05.80 Cartridge 27 filtering 370/1000ml at 0.20ml/s with 26.6psi vacuum 2.0m deep (17.6psia)
@03:39:05.79 Cartridge 27 filtering 383/1000ml at 0.24ml/s with 23.9psi vacuum 5.3m deep (22.5psia)
@03:40:05.80 Cartridge 27 filtering 395/1000ml at 0.20ml/s with 28.4psi vacuum 1.6m deep (17.1psia)
@03:41:05.79 Cartridge 27 filtering 408/1000ml at 0.20ml/s with 28.4psi vacuum 3.2m deep (19.4psia)
@03:42:05.80 Cartridge 27 filtering 420/1000ml at 0.22ml/s with 23.0psi vacuum 4.8m deep (21.8psia)
@03:43:05.79 Cartridge 27 filtering 432/1000ml at 0.20ml/s with 28.3psi vacuum 2.3m deep (18.2psia)
@03:44:05.80 Cartridge 27 filtering 445/1000ml at 0.20ml/s with 24.1psi vacuum 4.8m deep (21.8psia)
@03:44:41.28 <FILTERING> Flow averaged 0.25ml/s over 30:38
@03:44:41.31 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi
@03:44:41.87 Relieved excess vacuum in .6 seconds
@03:44:41.90 SamplePump.seek 1000ml,46:12
@03:44:52.86 Flow averaged 0.20ml/s over 11 seconds
@03:44:52.88 <closeIntake> Intake.close
@03:44:52.91 <closeExhaust> Exhaust.close
@03:44:53.10 <FILTERING> Toroid.in :hold
@03:44:53.16 Toroid.seek :bypass
@03:44:54.79 Toroid.in :default
@03:44:54.85 Toroid.seek :clear
@03:44:56.45 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@03:44:56.46 Pressure.coast
@03:44:56.65 Sampled  454.1ml
@03:44:58.77 <LRAUV> -> Cmd.startProcessing
@03:45:00.20 <PROCESSING> SPR.command \"read_sequences\"
@03:45:00.75 SPR.command \"stats_on\"
@03:45:00.79 SPR.command \"start 0 1\"
@03:45:02.80 <SPRlogger> Begun log /var/log/esp/spr21apr2303h.spr
@03:45:03.59 <PROCESSING> Handoff.select :waste
@03:45:10.27 Syringe tare is 20% of full scale
@03:45:10.29 PinPort.in :locking
@03:45:10.36 PinPort.seek :locked
@03:45:10.84 <posArchive> Archive.in :fast
@03:45:10.90 <posLyS2> Lysis2.in :fast
@03:45:10.93 <PROCESSING> Lysis1.seek 1350uL
@03:45:11.05 <posLyS2> Lysis2.seek :full
@03:45:11.10 <posArchive> Archive.seek :full
@03:45:13.14 <PROCESSING> Plunge.seek :reagent
@03:45:14.04 Evacuating puck with filter bubble point of 50psi
@03:45:14.05 PinPort.seek :locked
@03:45:14.14 Handoff.select :waste
@03:45:20.72 Air syringe pumping up to 1 stroke to reach 8psi
@03:45:20.73 Air stroke #1 of 1
@03:45:20.81 Air.in :pressurize
@03:45:20.87 Air.seek :pressurized
@03:45:32.14 3.5psi after pressurize
@03:45:32.15 Air.in :compress
@03:45:32.22 Air.seek :evac
@03:45:36.31 0.7psi after compress
@03:45:48.88 -0.0psi after compress (stable)
@03:45:48.90 Air.in :default
@03:45:48.97 Air.hold
@03:45:49.14 at -0.0psi Opened to waste
@03:45:49.15 Handoff.select :blocked
@03:45:49.28 Archive syringe pumping up to 5 strokes to reach 50psi
@03:45:49.29 Archive stroke #1 of 5
@03:45:49.36 Archive.in :pressurize
@03:45:49.43 Archive.seek :pressurized
@03:46:02.61 8.1psi after pressurize
@03:46:02.62 Archive.in :compress
@03:46:02.71 Archive.seek :empty
@03:46:18.60 24.2psi after compress
@03:47:00.67 44.1psi after compress (stable)
@03:47:00.69 Archive.in :gulp
@03:47:00.76 Archive.seek :topVent
@03:47:07.97 45.5psi after gulp
@03:47:20.56 46.1psi after gulp (stable)
@03:47:20.57 Archive stroke #2 of 5
@03:47:20.66 Archive.in :pressurize
@03:47:20.72 Archive.seek :pressurized
@03:47:33.45 46.0psi after pressurize
@03:47:33.46 Archive.in :compress
@03:47:33.55 Archive.seek :empty
@03:47:48.99 Reached 50.2 of 50.0psi after pumping 3451uL (1.99 strokes)
@03:47:49.00 Archive.in :default
@03:47:49.07 Archive.hold
@03:47:49.16 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position
@03:47:49.18 Handoff.select :waste
@03:47:58.87 delay 1:00
@03:48:58.89 Handoff.select :blocked
@03:49:21.57 Handoff.select :waste
@03:49:21.69 1.0psi at evacEnd
@03:49:21.70 Handoff.select :blocked
@03:49:21.83 Archive.in :fast
@03:49:21.90 Archive.seek :topVent
@03:49:35.58 Stable @1.8psi after Handoff blocked
@03:49:35.59 Archive syringe pumping up to 5 strokes to reach 32psi
@03:49:35.61 Archive stroke #1 of 5
@03:49:35.70 Archive.in :pressurize
@03:49:35.76 Archive.seek :pressurized
@03:49:48.49 2.4psi after pressurize
@03:49:48.50 Archive.in :compress
@03:49:48.58 Archive.seek :empty
@03:50:04.47 3.2psi after compress
@03:50:28.40 Reached 31.9 of 31.8psi after pumping 1730uL (1.00 strokes)
@03:50:28.41 Archive.in :default
@03:50:28.49 Archive.hold
@03:50:28.56 Handoff.select :waste
@03:50:42.74 delay 1:00
@03:51:42.77 Handoff.select :blocked
@03:52:03.00 Pressure too High, open to waste again (8.1psi)
@03:52:03.01 Handoff.select :waste
@03:52:03.14 delay 1:00
@03:53:03.16 Handoff.select :blocked
@03:53:33.34 Handoff.select :waste
@03:53:33.46 4.7psi at evacEnd
@03:53:33.47 Air.seek :fullVent
@03:53:34.92 Archive.in :fast
@03:53:34.98 Archive.seek :topVent
@03:53:42.13 Seawater evacuated, Presure above/below filter released. Lysis begins
@03:53:42.22 Lysis1.in :slow
@03:53:42.28 Lysis1.seek 1050uL
@03:54:12.31 delay 5 seconds
@03:54:17.33 Handoff.select :blocked
@03:54:17.52 Lysis1.seek 450uL
@03:55:17.49 delay 3 seconds
@03:55:20.56 Air.in to mix into 1.37806 parts Lysis1
@03:55:21.12 <Airmixer> Air.seek :evac
@03:55:21.17 <PROCESSING> Lysis1.seek 300uL
@03:55:36.28 Lysis1.seek :empty
@03:56:06.38 Lysis buffer delivered @15.9psi
@03:56:06.39 Polling Can every 2:00
@03:56:06.94 PuckHeater.configure :default #ambient 11.5C
@03:56:06.99 PuckHeater.seek 80.2C
@03:57:19.66 PuckHeater.hold 2:00,80.2C
@03:59:20.18 Polling Can every 2:00
@03:59:20.73 PuckHeater.configure :default #ambient 11.6C
@03:59:20.77 PuckHeater.hold 30 seconds,79.2C
@03:59:51.05 Polling Can every 2:00
@03:59:51.61 PuckHeater.configure :default #ambient 11.6C
@03:59:51.65 PuckHeater.hold 30 seconds,78.7C
@04:00:21.95 Polling Can every 2:00
@04:00:22.49 PuckHeater.configure :default #ambient 11.6C
@04:00:22.56 PuckHeater.hold 10:00,78.1C
@04:10:24.74 Lysis complete. Syringe pressure @17.7psi
@04:10:24.75 Handoff.select :blocked
@04:10:24.80 Archive syringe pumping up to 3 strokes to reach 40psi
@04:10:24.81 Archive stroke #1 of 3
@04:10:24.89 Archive.in :pressurize
@04:10:24.97 Archive.seek :pressurized
@04:10:37.70 17.4psi after pressurize
@04:10:37.71 Archive.in :compress
@04:10:37.79 Archive.seek :empty
@04:10:48.42 Reached 40.7 of 40.0psi after pumping 1618uL (0.94 strokes)
@04:10:48.43 Archive.in :default
@04:10:48.50 Archive.hold
@04:10:48.57 delay 2 seconds
@04:10:50.60 Archive.in :fast
@04:10:50.66 Archive.seek :full
@04:10:57.47 Lysis1.seek 138uL
@04:11:11.74 delay 1 second
@04:11:12.77 <fillArchive> Archive.seek :full
@04:11:12.79 <PROCESSING> Lysis1.seek :empty
@04:11:26.78 Initial air pressure @44.8psi
@04:11:26.85 Lysis1.in :extract
@04:11:26.94 Lysis1.seek 600uL,nil
@04:11:28.64 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@04:11:28.65 Archive stroke #1 of 6
@04:11:28.73 Archive.in :pressurize
@04:11:28.80 Archive.seek :pressurized
@04:11:41.57 15.6psi after pressurize
@04:11:41.59 Archive.in :compress
@04:11:41.66 Archive.seek :empty
@04:11:57.54 15.6psi after compress
@04:12:10.56 15.8psi after compress (stable)
@04:12:15.76 Recovering 3.3uL/s
@04:12:20.83 Archive.in :gulp
@04:12:20.89 Archive.seek :topVent
@04:12:28.09 15.8psi after gulp
@04:12:40.66 15.7psi after gulp (stable)
@04:12:40.67 Archive stroke #2 of 6
@04:12:40.81 Archive.in :pressurize
@04:12:40.87 Archive.seek :pressurized
@04:12:53.60 16.0psi after pressurize
@04:12:53.61 Archive.in :compress
@04:12:53.69 Archive.seek :empty
@04:13:05.69 Archive.in :default
@04:13:05.74 Archive.hold
@04:13:05.96 <PROCESSING> Initial 600uL lysate @16.5psi
@04:13:05.97 Handoff.select :blocked
@04:13:06.07 Initial air pressure @17.0psi
@04:13:06.14 Lysis1.seek 825uL,nil
@04:13:07.35 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@04:13:07.36 Archive stroke #1 of 6
@04:13:07.44 Archive.in :compress
@04:13:07.51 Archive.seek :empty
@04:13:08.14 Reached 17.2 of 17.0psi after pumping 1661uL (0.96 strokes)
@04:13:08.15 Archive.in :default
@04:13:08.22 Archive.hold
@04:13:08.94 Archive syringe pumping up to 6 strokes to reach 17psi
@04:13:08.95 Archive stroke #1 of 6
@04:13:09.08 Archive.in :compress
@04:13:09.15 Archive.seek :empty
@04:13:12.56 15.9psi after compress
@04:13:18.86 Archive.in :default
@04:13:18.91 Archive.hold
@04:13:19.11 <PROCESSING> Recovered 825uL lysate @16.1psi
@04:13:19.13 Air.in :halfSpeed
@04:13:19.20 Air.seek :fullVent
@04:13:21.45 Puck Top Vented, bottom @14.2psi
@04:13:21.46 Handoff.select :waste
@04:13:21.57 delay 5 seconds
@04:13:26.63 Handoff open to waste, bottom @0.1psi
@04:13:26.65 Air.in :default
@04:13:26.72 Air.seek :evac
@04:13:28.17 Lysis2.in :DAmix
@04:13:28.27 Lysis1.in :DAmix
@04:13:28.76 <Lysis2mixer> Lysis2.seek 1480uL
@04:13:28.81 <PROCESSING> Lysis1.seek 575uL
@04:14:03.86 delay 5 seconds
@04:14:08.88 Handoff.select SPR
@04:14:08.99 SPR.command \"start 2 14\"
@04:14:09.70 delay 10 seconds
@04:14:19.72 SPR.command \"start 1 0\"
@04:14:23.45 Waiting up to 4:00 for SPR to enter state 1
@04:16:26.28 SPR.command \"hold_on\"
@04:16:28.18 SPR Dilution 1 of 1 -- Running Experiment 1
@04:16:28.62 <Lysis2mixer> Lysis2.seek 1180uL
@04:16:28.65 <PROCESSING> Lysis1.seek 275uL
@04:17:10.69 volL1 = 300.0 and volL2 = 300.0
@04:17:10.71 delay 15 seconds
@04:17:25.75 SPR.command \"hold_off\"
@04:17:28.34 Waiting up to 38:00 for SPR to enter state 7
@04:44:10.94 SPR Analysis has Completed
@04:44:10.95 Handoff.select :waste
@04:44:11.88 <SPRlogger> Ended log /var/log/esp/spr21apr2303h.spr
@04:44:11.89 <PROCESSING> SPR.command \"stats_off\"
@04:44:13.32 <LRAUV> -> Cmd.stop
@04:44:13.41 <closeHandoff> Handoff.select :close
@04:44:13.54 <LRAUV> Gate.power :heat,:OFF
@04:44:13.56 Gate.power :cartridge,:OFF
@04:44:13.64 Carousel.seek 27
@04:44:13.81 Plunge.seek :locked
@04:44:14.69 PinPort.in :unlocking
@04:44:14.75 PinPort.seek :clear
@04:44:15.29 <Archive> Archive.in :unlocking
@04:44:15.43 Archive.seek :clear
@04:44:15.50 <Air> Air.in :unlocking
@04:44:15.60 Air.seek :clear
@04:44:15.68 <Lysis1> Lysis1.in :unlocking
@04:44:15.81 Lysis1.seek :clear
@04:44:15.88 <Lysis2> Lysis2.in :unlocking
@04:44:15.94 Lysis2.seek :clear
@04:44:19.47 <LRAUV> Archive.coast
@04:44:19.52 Air.coast
@04:44:19.57 Lysis1.coast
@04:44:19.62 Lysis2.coast
@04:44:19.68 Plunge.coast
@04:44:19.73 SamplePump.coast
@04:44:19.78 Toroid.coast
@04:44:19.83 Carousel.coast
@04:44:19.89 PinPort.coast
@04:44:20.07 Gate.power :motor,:OFF
@04:44:20.09 Gate.power :core,:OFF
@04:44:20.11 Gate.power :analytic1,:OFF
@04:44:20.13 Gate.power :analytic2,:OFF
@04:44:20.15 Gate.power :analytic3,:OFF
@04:44:20.17 Gate.power :main,:OFF
@04:44:20.19 Safely stopped and ready to power off
##-- file closed 2021-04-23T13:51:16.647Z

##-- file opened 2021-04-23T13:51:16.652Z
@06:51:17.44 -> showStatus
@06:51:19.46 -> Cmd.loadCartridge -6
@06:51:19.60 Gate.power :main,:ON,limit to 5000mA
@06:51:19.62 Gate.power :analytic1,:ON,limit to 5000mA
@06:51:19.64 Gate.power :analytic2,:ON,limit to 5000mA
@06:51:19.66 Gate.power :analytic3,:ON,limit to 5000mA
@06:51:19.80 Powering core bus
@06:51:22.08 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@06:51:22.21 <LRAUV> Archive.configure :default
@06:51:22.33 Air.configure :default
@06:51:22.43 Lysis1.configure :default
@06:51:22.54 Lysis2.configure :default
@06:51:22.66 Plunge.configure :default
@06:51:22.78 SamplePump.configure :default
@06:51:22.93 ExtraPump.configure :default
@06:51:23.03 Toroid.configure :default
@06:51:23.17 Carousel.configure :default
@06:51:23.29 Pressure.configure :default
@06:51:23.39 PinPort.configure :default
@06:51:23.45 All elf controllers running firmware version 4.741
@06:51:23.48 Gate.power :motor,:ON,limit to 5000mA
@06:51:23.65 <Archive_home> Archive.in :default
@06:51:24.28 Archive.home.jog 0
@06:51:24.44 <Toroid_home> Toroid.in :default
@06:51:24.51 <Plunge_home> Plunge.in :default
@06:51:24.54 <Lysis1_home> Lysis1.in :default
@06:51:24.60 <PinPort_home> PinPort.in :default
@06:51:24.65 <Lysis2_home> Lysis2.in :default
@06:51:24.68 <Air_home> Air.in :default
@06:51:24.87 <Plunge_home> Plunge.home.jog 0
@06:51:24.90 <Toroid_home> Toroid.home.jog 0
@06:51:24.93 <Lysis1_home> Lysis1.home.jog 0
@06:51:24.96 <Lysis2_home> Lysis2.home.jog 0
@06:51:24.98 <PinPort_home> PinPort.home.jog 0
@06:51:25.02 <Air_home> Air.home.jog 0
@06:51:25.53 <Archive_home> Archive.in :homing
@06:51:25.66 Archive.jog 1415
@06:51:25.73 <PinPort_home> PinPort.in :homing
@06:51:25.77 <Plunge_home> Plunge.in :homing
@06:51:25.81 <Toroid_home> Toroid.in :homing
@06:51:25.85 <Lysis1_home> Lysis1.in :homing
@06:51:25.89 <Air_home> Air.in :homing
@06:51:25.93 <Lysis2_home> Lysis2.in :homing
@06:51:26.10 <PinPort_home> PinPort.jog -107
@06:51:26.17 <Plunge_home> Plunge.jog 1577
@06:51:26.20 <Toroid_home> Toroid.jog 609
@06:51:26.26 <Lysis2_home> Lysis2.jog 1430
@06:51:26.27 <Lysis1_home> Lysis1.jog 1418
@06:51:26.30 <Air_home> Air.jog 169
@06:51:26.53 <PinPort_home> PinPort.setRawPosition! 0
@06:51:26.58 PinPort.seek :home
@06:51:26.66 PinPort.in :default
@06:51:26.85 <Air_home> Air.setRawPosition! 50
@06:51:26.90 Air.seek :home
@06:51:27.08 <Archive_home> Archive.setRawPosition! 50
@06:51:27.13 Archive.seek :home
@06:51:27.22 <Air_home> Air.in :default
@06:51:27.41 <Archive_home> Archive.in :default
@06:51:27.56 <Plunge_home> Plunge.setRawPosition! 50
@06:51:27.58 <Lysis2_home> Lysis2.setRawPosition! 50
@06:51:27.66 Lysis2.seek :home
@06:51:27.69 <Plunge_home> Plunge.seek :home
@06:51:27.82 <Lysis1_home> Lysis1.setRawPosition! 50
@06:51:27.87 Lysis1.seek :home
@06:51:27.99 <Lysis2_home> Lysis2.in :default
@06:51:28.07 <Plunge_home> Plunge.in :default
@06:51:28.16 <Lysis1_home> Lysis1.in :default
@06:51:28.41 <Toroid_home> Toroid.setRawPosition! 0
@06:51:28.46 Toroid.seek :home
@06:51:28.55 Toroid.in :default
@06:51:28.68 <LRAUV> Toroid.seek :clear
@06:51:30.30 Carousel.in :default
@06:51:30.36 Carousel.home.jog 0
@06:51:30.86 Carousel.in :default
@06:51:30.92 Carousel.seek 27
@06:51:31.08 Selecting Cartridge 30
@06:51:31.33 Carousel.seek 30
@06:51:32.22 Lysis1.in :fast
@06:51:32.28 Lysis1.seek :locked
@06:51:34.87 <Plunge> Plunge.seek :locked
@06:51:35.01 <Air> Air.in :fast
@06:51:35.08 Air.seek :locked
@06:51:37.69 <LRAUV> Powering cartridge bus
@06:51:39.81 Gate.setIR 1750,5000,0,6
@06:51:39.94 Gate.setIR 0,5000,0,6
@06:51:39.99 Gate.power :cartridge,:OFF
@06:51:40.53 Mounted archive Cartridge
@06:51:59.05 -> Cmd.startFiltering
@06:51:59.51 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@06:51:59.52 SamplePump.setPosition! 0ml
@06:51:59.56 Bypass.open
@06:52:01.04 Intake.open
@06:52:01.85 Sea 4.9m deep (21.9psia)
@06:52:01.86 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@06:52:04.45 Vacuum tare is 51% of full scale
@06:52:04.58 Pressure.in :default
@06:52:04.64 Pressure.hold
@06:52:04.81 SamplePump.in :prime
@06:52:04.89 SamplePump.seek 10ml,1:21
@06:52:14.30 Pressure.coast
@06:52:14.35 SamplePump.setPosition! 0ml
@06:52:14.40 Exhaust.open
@06:52:15.83 <closeBypass> Bypass.close
@06:52:17.37 <FILTERING> Pressure.in :default
@06:52:17.43 Pressure.hold
@06:52:17.54 Sea 4.3m deep (21.1psia)
@06:52:17.67 SamplePump.in :prime
@06:52:17.74 SamplePump.seek 100ml,8:51
@06:54:27.94 Pressure.coast
@06:54:27.99 Bypass.open
@06:54:28.70 Intake.close
@06:54:29.55 Toroid.in :hold
@06:54:29.61 Toroid.seek :crack
@06:54:31.17 Toroid.seek :cartridge
@06:54:32.27 Toroid.in :default
@06:54:32.33 Toroid.seek :clear
@06:54:34.30 Pressurizing cartridge to 26psi above 21.3psia
@06:54:34.31 SamplePump.setPosition! 0ml
@06:54:34.35 Intake.open
@06:54:35.08 Bypass.close
@06:54:35.81 Exhaust.close
@06:54:36.62 Pressure.in :default
@06:54:36.68 Pressure.hold
@06:54:36.85 SamplePump.in :charge
@06:54:36.92 SamplePump.seek 2.3ml,42 seconds
@06:54:42.93 Pressure.coast
@06:54:42.99 Intake.close
@06:54:43.78 Pressurized cartridge with 0.159ml to 26.1psi
@06:54:43.79 Isolated @41.7psia (20.4psi)
@06:54:43.80 Verifying cartridge holds at least 0.75..0.85 of 41.7psia
@06:54:43.83 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@06:54:48.40 Cartridge pressure fell 1% to 41.4psia
@06:54:48.48 Sampling 1000.0ml
@06:54:48.49 SamplePump.setPosition! 0ml
@06:54:48.54 Exhaust.open
@06:54:49.29 Intake.open
@06:54:50.12 Pressure.in :default
@06:54:50.18 Pressure.hold
@06:54:50.30 Sea 4.3m deep (21.0psia)
@06:54:50.84 SamplePump.in :sample
@06:54:50.91 SamplePump.seek 1000ml,1:23:53
@06:54:53.15 <sampling> Cartridge 30 filtering 1/1000ml at 0.38ml/s with 23.1psi vacuum 2.7m deep (18.6psia)
@06:55:53.16 Cartridge 30 filtering 20/1000ml at 0.36ml/s with 27.1psi vacuum 4.6m deep (21.4psia)
@06:56:53.16 Cartridge 30 filtering 39/1000ml at 0.35ml/s with 24.5psi vacuum 3.9m deep (20.5psia)
@06:57:53.16 Cartridge 30 filtering 58/1000ml at 0.33ml/s with 24.2psi vacuum 4.8m deep (21.7psia)
@06:58:53.16 Cartridge 30 filtering 76/1000ml at 0.30ml/s with 18.8psi vacuum 5.5m deep (22.8psia)
@06:59:53.16 Cartridge 30 filtering 95/1000ml at 0.28ml/s with 18.9psi vacuum 3.2m deep (19.4psia)
@07:00:53.16 Cartridge 30 filtering 113/1000ml at 0.25ml/s with 22.6psi vacuum 3.7m deep (20.2psia)
@07:01:53.16 Cartridge 30 filtering 130/1000ml at 0.31ml/s with 23.3psi vacuum 3.1m deep (19.3psia)
@07:02:53.16 Cartridge 30 filtering 148/1000ml at 0.27ml/s with 30.4psi vacuum 3.6m deep (19.9psia)
@07:03:53.16 Cartridge 30 filtering 165/1000ml at 0.30ml/s with 18.7psi vacuum 4.6m deep (21.4psia)
@07:04:53.16 Cartridge 30 filtering 182/1000ml at 0.30ml/s with 23.7psi vacuum 4.9m deep (22.0psia)
@07:05:53.16 Cartridge 30 filtering 199/1000ml at 0.29ml/s with 23.7psi vacuum
@07:06:53.16 Cartridge 30 filtering 215/1000ml at 0.23ml/s with 24.0psi vacuum 1.9m deep (17.5psia)
@07:07:53.16 Cartridge 30 filtering 232/1000ml at 0.27ml/s with 25.1psi vacuum 5.0m deep (22.1psia)
@07:08:53.16 Cartridge 30 filtering 248/1000ml at 0.22ml/s with 27.0psi vacuum
@07:09:53.16 Cartridge 30 filtering 264/1000ml at 0.28ml/s with 24.5psi vacuum 3.4m deep (19.7psia)
@07:10:53.16 Cartridge 30 filtering 279/1000ml at 0.22ml/s with 21.5psi vacuum 3.1m deep (19.3psia)
@07:11:53.16 Cartridge 30 filtering 295/1000ml at 0.22ml/s with 20.4psi vacuum 5.3m deep (22.6psia)
@07:12:53.16 Cartridge 30 filtering 310/1000ml at 0.26ml/s with 25.4psi vacuum 1.7m deep (17.3psia)
@07:13:53.16 Cartridge 30 filtering 325/1000ml at 0.27ml/s with 23.2psi vacuum 5.2m deep (22.4psia)
@07:14:53.16 Cartridge 30 filtering 340/1000ml at 0.25ml/s with 24.8psi vacuum 1.9m deep (17.5psia)
@07:15:53.16 Cartridge 30 filtering 355/1000ml at 0.23ml/s with 22.1psi vacuum 2.0m deep (17.7psia)
@07:16:53.16 Cartridge 30 filtering 370/1000ml at 0.25ml/s with 25.1psi vacuum 3.6m deep (20.0psia)
@07:17:53.16 Cartridge 30 filtering 384/1000ml at 0.22ml/s with 21.5psi vacuum 2.5m deep (18.5psia)
@07:18:53.16 Cartridge 30 filtering 399/1000ml at 0.25ml/s with 24.1psi vacuum 4.1m deep (20.8psia)
@07:19:53.16 Cartridge 30 filtering 413/1000ml at 0.25ml/s with 23.9psi vacuum 2.3m deep (18.1psia)
@07:20:53.16 Cartridge 30 filtering 427/1000ml at 0.21ml/s with 26.9psi vacuum 4.3m deep (21.1psia)
@07:21:53.16 Cartridge 30 filtering 441/1000ml at 0.21ml/s with 23.3psi vacuum 2.7m deep (18.7psia)
@07:22:53.16 Cartridge 30 filtering 455/1000ml at 0.21ml/s with 27.5psi vacuum 3.7m deep (20.1psia)
@07:23:53.16 Cartridge 30 filtering 469/1000ml at 0.24ml/s with 25.3psi vacuum
@07:24:53.16 Cartridge 30 filtering 483/1000ml at 0.23ml/s with 22.9psi vacuum 4.1m deep (20.7psia)
@07:25:53.16 Cartridge 30 filtering 496/1000ml at 0.21ml/s with 23.0psi vacuum 3.9m deep (20.5psia)
@07:26:53.16 Cartridge 30 filtering 510/1000ml at 0.26ml/s with 23.4psi vacuum 4.1m deep (20.7psia)
@07:27:53.16 Cartridge 30 filtering 523/1000ml at 0.24ml/s with 18.5psi vacuum 6.2m deep (23.8psia)
@07:28:53.16 Cartridge 30 filtering 536/1000ml at 0.23ml/s with 26.0psi vacuum 2.1m deep (17.8psia)
@07:29:53.16 Cartridge 30 filtering 549/1000ml at 0.23ml/s with 18.2psi vacuum 5.8m deep (23.3psia)
@07:30:53.16 Cartridge 30 filtering 563/1000ml at 0.21ml/s with 24.8psi vacuum 2.3m deep (18.1psia)
@07:31:53.16 Cartridge 30 filtering 575/1000ml at 0.20ml/s with 24.6psi vacuum 4.2m deep (20.9psia)
@07:32:53.16 Cartridge 30 filtering 588/1000ml at 0.21ml/s with 22.5psi vacuum 4.8m deep (21.9psia)
@07:33:53.16 Cartridge 30 filtering 601/1000ml at 0.22ml/s with 25.3psi vacuum 2.5m deep (18.4psia)
@07:34:53.16 Cartridge 30 filtering 614/1000ml at 0.20ml/s with 24.3psi vacuum 3.9m deep (20.4psia)
@07:35:53.16 Cartridge 30 filtering 626/1000ml at 0.24ml/s with 23.5psi vacuum 3.7m deep (20.1psia)
@07:36:53.16 Cartridge 30 filtering 639/1000ml at 0.20ml/s with 25.0psi vacuum 3.6m deep (20.0psia)
@07:37:53.16 Cartridge 30 filtering 651/1000ml at 0.21ml/s with 23.7psi vacuum 5.0m deep (22.0psia)
@07:38:53.16 Cartridge 30 filtering 664/1000ml at 0.20ml/s with 26.2psi vacuum 2.9m deep (18.9psia)
@07:39:53.16 Cartridge 30 filtering 676/1000ml at 0.22ml/s with 18.2psi vacuum 5.8m deep (23.2psia)
@07:40:53.16 Cartridge 30 filtering 689/1000ml at 0.20ml/s with 26.2psi vacuum 3.9m deep (20.4psia)
@07:41:10.76 <FILTERING> Flow averaged 0.25ml/s over 46:20
@07:41:10.79 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi
@07:41:11.37 Relieved excess vacuum in .6 seconds
@07:41:11.39 SamplePump.seek 1000ml,26:10
@07:41:20.54 Flow averaged 0.20ml/s over 10 seconds
@07:41:20.56 <closeIntake> Intake.close
@07:41:20.63 <closeExhaust> Exhaust.close
@07:41:20.79 <FILTERING> Toroid.in :hold
@07:41:20.85 Toroid.seek :bypass
@07:41:22.48 Toroid.in :default
@07:41:22.54 Toroid.seek :clear
@07:41:24.16 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@07:41:24.17 Pressure.coast
@07:41:24.36 Sampled  694.0ml
@07:41:26.58 <LRAUV> -> Cmd.startProcessing
@07:41:26.95 <PROCESSING> Plunge.seek :reagent
@07:41:27.79 Sample loop closed. Ready to Deliver Reagents
@07:41:27.80 Archive.seek :sealed
@07:41:32.08 Archive.seek :evac
@07:41:46.03 delay 1:00
@07:42:46.06 Archive.seek :clear
@07:43:03.78 RNAlater soak for 10:00
@07:53:03.80 Archive.seek :tVent
@07:53:06.38 delay 1 second
@07:53:07.40 Archive.seek :evac
@07:53:23.02 delay 10 seconds
@07:53:33.05 Sample preserved and evacuated
@07:53:34.23 <LRAUV> -> Cmd.stop
@07:53:34.37 Carousel.seek 30
@07:53:34.54 Plunge.seek :locked
@07:53:35.48 <Archive> Archive.in :unlocking
@07:53:35.61 Archive.seek :clear
@07:53:35.67 <Air> Air.in :unlocking
@07:53:35.81 Air.seek :clear
@07:53:35.87 <Lysis1> Lysis1.in :unlocking
@07:53:36.01 Lysis1.seek :clear
@07:53:39.66 <LRAUV> Archive.coast
@07:53:39.71 Air.coast
@07:53:39.77 Lysis1.coast
@07:53:39.81 Lysis2.coast
@07:53:39.87 Plunge.coast
@07:53:39.92 SamplePump.coast
@07:53:39.97 Toroid.coast
@07:53:40.02 Carousel.coast
@07:53:40.07 PinPort.coast
@07:53:40.25 Gate.power :motor,:OFF
@07:53:40.26 Gate.power :core,:OFF
@07:53:40.29 Gate.power :analytic1,:OFF
@07:53:40.31 Gate.power :analytic2,:OFF
@07:53:40.33 Gate.power :analytic3,:OFF
@07:53:40.35 Gate.power :main,:OFF
@07:53:40.37 Safely stopped and ready to power off
##-- file closed 2021-04-23T15:08:17.402Z

##-- file opened 2021-04-23T15:08:17.403Z
@08:08:17.88 -> showStatus
@08:08:19.90 -> Cmd.loadCartridge -9
@08:08:20.04 Gate.power :main,:ON,limit to 5000mA
@08:08:20.06 Gate.power :analytic1,:ON,limit to 5000mA
@08:08:20.09 Gate.power :analytic2,:ON,limit to 5000mA
@08:08:20.13 Gate.power :analytic3,:ON,limit to 5000mA
@08:08:20.26 Powering core bus
@08:08:22.54 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@08:08:22.67 <LRAUV> Archive.configure :default
@08:08:22.79 Air.configure :default
@08:08:22.90 Lysis1.configure :default
@08:08:23.00 Lysis2.configure :default
@08:08:23.12 Plunge.configure :default
@08:08:23.25 SamplePump.configure :default
@08:08:23.40 ExtraPump.configure :default
@08:08:23.51 Toroid.configure :default
@08:08:23.63 Carousel.configure :default
@08:08:23.75 Pressure.configure :default
@08:08:23.87 PinPort.configure :default
@08:08:23.93 All elf controllers running firmware version 4.741
@08:08:23.96 Gate.power :motor,:ON,limit to 5000mA
@08:08:24.09 <Archive_home> Archive.in :default
@08:08:24.75 Archive.home.jog 0
@08:08:24.92 <PinPort_home> PinPort.in :default
@08:08:25.00 <Toroid_home> Toroid.in :default
@08:08:25.04 <Lysis2_home> Lysis2.in :default
@08:08:25.07 <Air_home> Air.in :default
@08:08:25.11 <Lysis1_home> Lysis1.in :default
@08:08:25.15 <Plunge_home> Plunge.in :default
@08:08:25.36 <PinPort_home> PinPort.home.jog 0
@08:08:25.38 <Toroid_home> Toroid.home.jog 0
@08:08:25.41 <Lysis2_home> Lysis2.home.jog 0
@08:08:25.44 <Lysis1_home> Lysis1.home.jog 0
@08:08:25.46 <Air_home> Air.home.jog 0
@08:08:25.50 <Plunge_home> Plunge.home.jog 0
@08:08:25.95 <Archive_home> Archive.in :homing
@08:08:26.18 <PinPort_home> PinPort.in :homing
@08:08:26.22 <Toroid_home> Toroid.in :homing
@08:08:26.25 <Lysis1_home> Lysis1.in :homing
@08:08:26.29 <Air_home> Air.in :homing
@08:08:26.32 <Plunge_home> Plunge.in :homing
@08:08:26.36 <Lysis2_home> Lysis2.in :homing
@08:08:26.39 <Archive_home> Archive.jog 155
@08:08:26.60 <PinPort_home> PinPort.jog -107
@08:08:26.63 <Toroid_home> Toroid.jog 607
@08:08:26.66 <Air_home> Air.jog 154
@08:08:26.69 <Lysis1_home> Lysis1.jog 1416
@08:08:26.72 <Lysis2_home> Lysis2.jog 1430
@08:08:26.75 <Plunge_home> Plunge.jog 1577
@08:08:27.01 <PinPort_home> PinPort.setRawPosition! 0
@08:08:27.03 <Archive_home> Archive.setRawPosition! 50
@08:08:27.11 Archive.seek :home
@08:08:27.14 <PinPort_home> PinPort.seek :home
@08:08:27.28 <Air_home> Air.setRawPosition! 50
@08:08:27.30 <PinPort_home> PinPort.in :default
@08:08:27.38 <Air_home> Air.seek :home
@08:08:27.49 <Archive_home> Archive.in :default
@08:08:27.68 <Air_home> Air.in :default
@08:08:28.02 <Lysis1_home> Lysis1.setRawPosition! 50
@08:08:28.07 Lysis1.seek :home
@08:08:28.17 <Lysis2_home> Lysis2.setRawPosition! 50
@08:08:28.19 <Plunge_home> Plunge.setRawPosition! 50
@08:08:28.26 Plunge.seek :home
@08:08:28.29 <Lysis2_home> Lysis2.seek :home
@08:08:28.42 <Lysis1_home> Lysis1.in :default
@08:08:28.58 <Plunge_home> Plunge.in :default
@08:08:28.66 <Lysis2_home> Lysis2.in :default
@08:08:28.89 <Toroid_home> Toroid.setRawPosition! 0
@08:08:28.94 Toroid.seek :home
@08:08:29.03 Toroid.in :default
@08:08:29.16 <LRAUV> Toroid.seek :clear
@08:08:30.78 Carousel.in :default
@08:08:30.84 Carousel.home.jog 0
@08:08:31.34 Carousel.in :default
@08:08:31.40 Carousel.seek 30
@08:08:31.54 Selecting Cartridge 25
@08:08:31.79 Carousel.seek 25
@08:08:33.08 Lysis1.in :fast
@08:08:33.15 Lysis1.seek :locked
@08:08:35.74 <Plunge> Plunge.seek :locked
@08:08:35.86 <Air> Air.in :fast
@08:08:35.92 Air.seek :locked
@08:08:38.54 <LRAUV> Powering cartridge bus
@08:08:40.66 Gate.setIR 1750,5000,0,6
@08:08:40.79 Gate.setIR 0,5000,0,6
@08:08:40.85 PuckHeater.configure :default
@08:08:40.90 Gate.power :heat,:ON,limit to 5000mA
@08:08:41.56 Mounted lysis Cartridge board#Q05030
@08:08:41.60 Using default puck heat loss curve for HeatingAccelInCarousel
@08:09:00.30 -> Cmd.startFiltering
@08:09:00.76 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@08:09:00.77 SamplePump.setPosition! 0ml
@08:09:00.81 Bypass.open
@08:09:02.32 Intake.open
@08:09:03.13 Sea 4.1m deep (20.8psia)
@08:09:03.15 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@08:09:05.72 Vacuum tare is 51% of full scale
@08:09:05.84 Pressure.in :default
@08:09:05.90 Pressure.hold
@08:09:06.07 SamplePump.in :prime
@08:09:06.19 SamplePump.seek 10ml,1:21
@08:09:15.59 Pressure.coast
@08:09:15.64 SamplePump.setPosition! 0ml
@08:09:15.69 Exhaust.open
@08:09:17.10 <closeBypass> Bypass.close
@08:09:18.64 <FILTERING> Pressure.in :default
@08:09:18.70 Pressure.hold
@08:09:18.82 Sea 3.9m deep (20.5psia)
@08:09:18.95 SamplePump.in :prime
@08:09:19.02 SamplePump.seek 100ml,8:51
@08:11:29.41 Pressure.coast
@08:11:29.47 Bypass.open
@08:11:30.17 Intake.close
@08:11:31.02 Toroid.in :hold
@08:11:31.08 Toroid.seek :crack
@08:11:32.63 Toroid.seek :cartridge
@08:11:33.73 Toroid.in :default
@08:11:33.79 Toroid.seek :clear
@08:11:35.76 Pressurizing cartridge to 26psi above 20.7psia
@08:11:35.77 SamplePump.setPosition! 0ml
@08:11:35.81 Intake.open
@08:11:35.99 Bypass.close
@08:11:36.70 Exhaust.close
@08:11:37.51 Pressure.in :default
@08:11:37.57 Pressure.hold
@08:11:37.74 SamplePump.in :charge
@08:11:37.80 SamplePump.seek 2.3ml,42 seconds
@08:11:47.53 Pressure.coast
@08:11:47.58 Intake.close
@08:11:48.45 Pressurized cartridge with 0.420ml to 26.7psi
@08:11:48.46 Isolated @43.0psia (22.3psi)
@08:11:48.47 Verifying cartridge holds at least 0.75..0.85 of 43.0psia
@08:11:48.50 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@08:11:53.12 Sampling 1000.0ml
@08:11:53.14 SamplePump.setPosition! 0ml
@08:11:53.19 Exhaust.open
@08:11:53.92 Intake.open
@08:11:55.19 Pressure.in :default
@08:11:55.25 Pressure.hold
@08:11:55.37 Sea 5.5m deep (22.9psia)
@08:11:55.50 SamplePump.in :sample
@08:11:55.57 SamplePump.seek 1000ml,1:23:53
@08:11:57.81 <sampling> Cartridge 25 filtering 1/1000ml at 0.32ml/s with 22.4psi vacuum 3.1m deep (19.4psia)
@08:12:57.81 Cartridge 25 filtering 19/1000ml at 0.30ml/s with 23.3psi vacuum 3.4m deep (19.7psia)
@08:13:57.81 Cartridge 25 filtering 38/1000ml at 0.32ml/s with 21.8psi vacuum 3.1m deep (19.3psia)
@08:14:57.81 Cartridge 25 filtering 56/1000ml at 0.28ml/s with 22.8psi vacuum 4.3m deep (21.1psia)
@08:15:57.81 Cartridge 25 filtering 73/1000ml at 0.28ml/s with 26.6psi vacuum
@08:16:57.81 Cartridge 25 filtering 90/1000ml at 0.29ml/s with 21.8psi vacuum 4.2m deep (20.9psia)
@08:17:57.81 Cartridge 25 filtering 107/1000ml at 0.25ml/s with 26.9psi vacuum 1.9m deep (17.6psia)
@08:18:57.81 Cartridge 25 filtering 124/1000ml at 0.31ml/s with 24.7psi vacuum 4.4m deep (21.2psia)
@08:19:57.81 Cartridge 25 filtering 140/1000ml at 0.28ml/s with 20.1psi vacuum 4.8m deep (21.7psia)
@08:20:57.81 Cartridge 25 filtering 156/1000ml at 0.29ml/s with 21.2psi vacuum 3.1m deep (19.2psia)
@08:21:57.81 Cartridge 25 filtering 172/1000ml at 0.30ml/s with 24.7psi vacuum 5.6m deep (23.0psia)
@08:22:57.81 Cartridge 25 filtering 188/1000ml at 0.23ml/s with 22.1psi vacuum 2.7m deep (18.6psia)
@08:23:57.81 Cartridge 25 filtering 203/1000ml at 0.28ml/s with 25.6psi vacuum 5.7m deep (23.2psia)
@08:24:57.81 Cartridge 25 filtering 218/1000ml at 0.29ml/s with 23.8psi vacuum 3.0m deep (19.1psia)
@08:25:57.81 Cartridge 25 filtering 233/1000ml at 0.27ml/s with 25.7psi vacuum 4.2m deep (21.0psia)
@08:26:57.81 Cartridge 25 filtering 248/1000ml at 0.21ml/s with 24.7psi vacuum 2.4m deep (18.2psia)
@08:27:57.81 Cartridge 25 filtering 262/1000ml at 0.27ml/s with 24.2psi vacuum 4.1m deep (20.7psia)
@08:28:57.81 Cartridge 25 filtering 277/1000ml at 0.27ml/s with 22.0psi vacuum 4.1m deep (20.8psia)
@08:29:57.81 Cartridge 25 filtering 291/1000ml at 0.26ml/s with 25.8psi vacuum 2.6m deep (18.6psia)
@08:30:57.81 Cartridge 25 filtering 305/1000ml at 0.23ml/s with 21.9psi vacuum 4.4m deep (21.1psia)
@08:31:57.81 Cartridge 25 filtering 318/1000ml at 0.21ml/s with 22.6psi vacuum 2.9m deep (19.0psia)
@08:32:57.81 Cartridge 25 filtering 332/1000ml at 0.26ml/s with 24.2psi vacuum 5.8m deep (23.2psia)
@08:33:57.81 Cartridge 25 filtering 345/1000ml at 0.24ml/s with 23.3psi vacuum 1.9m deep (17.5psia)
@08:34:57.81 Cartridge 25 filtering 358/1000ml at 0.20ml/s with 26.9psi vacuum 4.2m deep (21.0psia)
@08:35:57.81 Cartridge 25 filtering 371/1000ml at 0.22ml/s with 26.7psi vacuum 2.5m deep (18.4psia)
@08:36:57.81 Cartridge 25 filtering 384/1000ml at 0.20ml/s with 23.4psi vacuum 4.2m deep (20.9psia)
@08:37:57.81 Cartridge 25 filtering 397/1000ml at 0.22ml/s with 23.9psi vacuum 3.2m deep (19.4psia)
@08:38:57.81 Cartridge 25 filtering 410/1000ml at 0.20ml/s with 26.0psi vacuum 2.4m deep (18.3psia)
@08:39:57.81 Cartridge 25 filtering 422/1000ml at 0.22ml/s with 22.7psi vacuum 4.8m deep (21.7psia)
@08:40:57.81 Cartridge 25 filtering 435/1000ml at 0.20ml/s with 23.4psi vacuum
@08:41:57.81 Cartridge 25 filtering 447/1000ml at 0.20ml/s with 26.0psi vacuum 4.1m deep (20.8psia)
@08:42:57.81 Cartridge 25 filtering 459/1000ml at 0.20ml/s with 22.5psi vacuum 2.5m deep (18.4psia)
@08:43:57.81 Cartridge 25 filtering 472/1000ml at 0.21ml/s with 23.4psi vacuum 4.0m deep (20.6psia)
@08:44:57.81 Cartridge 25 filtering 484/1000ml at 0.21ml/s with 24.1psi vacuum 3.7m deep (20.2psia)
@08:45:49.70 <FILTERING> Flow averaged 0.24ml/s over 33:55
@08:45:49.73 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi
@08:45:50.55 Relieved excess vacuum in .9 seconds
@08:45:50.58 SamplePump.seek 1000ml,42:41
@08:45:57.81 <sampling> Cartridge 25 filtering 496/1000ml at 0.20ml/s with 28.3psi vacuum 1.7m deep (17.3psia)
@08:46:28.25 <FILTERING> Flow averaged 0.20ml/s over 38 seconds
@08:46:28.28 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi
@08:46:28.86 Relieved excess vacuum in .6 seconds
@08:46:28.88 SamplePump.seek 1000ml,42:03
@08:46:36.40 Flow estimate reduced to 0.16ml/s
@08:46:36.41 <closeIntake> Intake.close
@08:46:36.47 <closeExhaust> Exhaust.close
@08:46:36.66 <FILTERING> Toroid.in :hold
@08:46:36.72 Toroid.seek :bypass
@08:46:38.35 Toroid.in :default
@08:46:38.41 Toroid.seek :clear
@08:46:40.03 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@08:46:40.04 Pressure.coast
@08:46:40.23 Sampled  503.3ml
@08:46:42.39 <LRAUV> -> Cmd.startProcessing
@08:46:43.83 <PROCESSING> SPR.command \"read_sequences\"
@08:46:44.58 SPR.command \"stats_on\"
@08:46:44.62 SPR.command \"start 0 1\"
@08:46:46.63 <SPRlogger> Begun log /var/log/esp/spr21apr2308h.spr
@08:46:47.44 <PROCESSING> Handoff.select :waste
@08:46:54.12 Syringe tare is 20% of full scale
@08:46:54.15 PinPort.in :locking
@08:46:54.22 PinPort.seek :locked
@08:46:54.70 <posArchive> Archive.in :fast
@08:46:54.76 <posLyS2> Lysis2.in :fast
@08:46:54.80 <PROCESSING> Lysis1.seek 1350uL
@08:46:54.92 <posArchive> Archive.seek :full
@08:46:54.96 <posLyS2> Lysis2.seek :full
@08:46:57.04 <PROCESSING> Plunge.seek :reagent
@08:46:57.94 Evacuating puck with filter bubble point of 50psi
@08:46:57.96 PinPort.seek :locked
@08:46:58.04 Handoff.select :waste
@08:47:04.63 Air syringe pumping up to 1 stroke to reach 8psi
@08:47:04.64 Air stroke #1 of 1
@08:47:04.73 Air.in :pressurize
@08:47:04.79 Air.seek :pressurized
@08:47:16.04 -0.1psi after pressurize
@08:47:16.05 Air.in :compress
@08:47:16.12 Air.seek :evac
@08:47:20.22 0.1psi after compress
@08:47:32.80 0.1psi after compress (stable)
@08:47:32.81 Air.in :default
@08:47:32.88 Air.hold
@08:47:33.05 at 0.1psi Opened to waste
@08:47:33.06 Handoff.select :blocked
@08:47:33.19 Archive syringe pumping up to 5 strokes to reach 50psi
@08:47:33.20 Archive stroke #1 of 5
@08:47:33.29 Archive.in :pressurize
@08:47:33.37 Archive.seek :pressurized
@08:47:46.11 9.9psi after pressurize
@08:47:46.13 Archive.in :compress
@08:47:46.20 Archive.seek :empty
@08:48:02.09 30.6psi after compress
@08:48:16.16 35.0psi after compress (stable)
@08:48:16.18 Archive.in :gulp
@08:48:16.26 Archive.seek :topVent
@08:48:23.48 35.1psi after gulp
@08:48:36.07 35.0psi after gulp (stable)
@08:48:36.08 Archive stroke #2 of 5
@08:48:36.16 Archive.in :pressurize
@08:48:36.22 Archive.seek :pressurized
@08:48:48.98 34.8psi after pressurize
@08:48:49.00 Archive.in :compress
@08:48:49.08 Archive.seek :empty
@08:49:03.26 Reached 50.2 of 50.0psi after pumping 3424uL (1.98 strokes)
@08:49:03.28 Archive.in :default
@08:49:03.35 Archive.hold
@08:49:03.42 Handoff.select :waste
@08:49:12.62 delay 1:00
@08:50:12.64 Handoff.select :blocked
@08:50:35.31 Handoff.select :waste
@08:50:35.43 0.5psi at evacEnd
@08:50:35.44 Handoff.select :blocked
@08:50:35.57 Archive.in :fast
@08:50:35.64 Archive.seek :topVent
@08:50:49.22 Stable @1.0psi after Handoff blocked
@08:50:49.24 Archive syringe pumping up to 5 strokes to reach 31psi
@08:50:49.26 Archive stroke #1 of 5
@08:50:49.35 Archive.in :pressurize
@08:50:49.42 Archive.seek :pressurized
@08:51:02.15 1.4psi after pressurize
@08:51:02.17 Archive.in :compress
@08:51:02.24 Archive.seek :empty
@08:51:18.12 3.1psi after compress
@08:52:05.29 Reached 31.0 of 31.0psi after pumping 1730uL (1.00 strokes)
@08:52:05.30 Archive.in :default
@08:52:05.37 Archive.hold
@08:52:05.43 Handoff.select :waste
@08:52:19.13 delay 1:00
@08:53:19.15 Handoff.select :blocked
@08:53:45.82 Handoff.select :waste
@08:53:45.94 3.8psi at evacEnd
@08:53:45.97 Air.seek :fullVent
@08:53:47.42 Archive.in :fast
@08:53:47.48 Archive.seek :topVent
@08:53:54.63 Seawater evacuated, Presure above/below filter released. Lysis begins
@08:53:54.72 Lysis1.in :slow
@08:53:54.78 Lysis1.seek 1050uL
@08:54:24.82 delay 5 seconds
@08:54:29.84 Handoff.select :blocked
@08:54:30.01 Lysis1.seek 450uL
@08:55:29.99 delay 3 seconds
@08:55:33.07 Air.in to mix into 1.37806 parts Lysis1
@08:55:33.54 <Airmixer> Air.seek :evac
@08:55:33.58 <PROCESSING> Lysis1.seek 300uL
@08:55:48.70 Lysis1.seek :empty
@08:56:18.80 Lysis buffer delivered @20.2psi
@08:56:18.81 Polling Can every 2:00
@08:56:19.36 PuckHeater.configure :default #ambient 11.4C
@08:56:19.41 PuckHeater.seek 80.2C
@08:57:27.86 PuckHeater.hold 2:00,80.2C
@08:59:28.07 Polling Can every 2:00
@08:59:28.62 PuckHeater.configure :default #ambient 11.4C
@08:59:28.67 PuckHeater.hold 30 seconds,79.2C
@08:59:58.88 Polling Can every 2:00
@08:59:59.43 PuckHeater.configure :default #ambient 11.4C
@08:59:59.48 PuckHeater.hold 30 seconds,78.7C
@09:00:29.69 Polling Can every 2:00
@09:00:30.24 PuckHeater.configure :default #ambient 11.4C
@09:00:30.29 PuckHeater.hold 10:00,78.1C
@09:10:30.49 Lysis complete. Syringe pressure @23.9psi
@09:10:30.50 Handoff.select :blocked
@09:10:30.54 Archive syringe pumping up to 3 strokes to reach 40psi
@09:10:30.55 Archive stroke #1 of 3
@09:10:30.66 Archive.in :pressurize
@09:10:30.72 Archive.seek :pressurized
@09:10:43.45 23.3psi after pressurize
@09:10:43.47 Archive.in :compress
@09:10:43.54 Archive.seek :empty
@09:10:53.19 Reached 40.6 of 40.0psi after pumping 1596uL (0.92 strokes)
@09:10:53.21 Archive.in :default
@09:10:53.28 Archive.hold
@09:10:53.35 delay 2 seconds
@09:10:55.37 Archive.in :fast
@09:10:55.43 Archive.seek :full
@09:11:02.18 Lysis1.seek 138uL
@09:11:16.05 delay 1 second
@09:11:17.08 <fillArchive> Archive.seek :full
@09:11:17.10 <PROCESSING> Lysis1.seek :empty
@09:11:31.08 Initial air pressure @52.5psi
@09:11:31.14 Lysis1.in :extract
@09:11:31.23 Lysis1.seek 600uL,nil
@09:11:34.61 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@09:11:34.62 Archive stroke #1 of 6
@09:11:34.71 Archive.in :pressurize
@09:11:34.80 Archive.seek :pressurized
@09:11:47.56 15.5psi after pressurize
@09:11:47.57 Archive.in :compress
@09:11:47.64 Archive.seek :empty
@09:12:02.13 Archive.in :default
@09:12:02.18 Archive.hold
@09:12:02.40 <PROCESSING> Initial 600uL lysate @18.7psi
@09:12:02.41 Handoff.select :blocked
@09:12:02.51 Initial air pressure @20.0psi
@09:12:02.58 Lysis1.seek 825uL,nil
@09:12:03.79 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@09:12:03.80 Archive stroke #1 of 6
@09:12:03.89 Archive.in :compress
@09:12:03.96 Archive.seek :empty
@09:12:05.34 16.5psi after compress
@09:12:17.97 15.8psi after compress (stable)
@09:12:23.10 Archive.in :gulp
@09:12:23.16 Archive.seek :topVent
@09:12:30.39 15.7psi after gulp
@09:12:42.96 15.8psi after gulp (stable)
@09:12:42.97 Archive stroke #2 of 6
@09:12:43.06 Archive.in :pressurize
@09:12:43.12 Archive.seek :pressurized
@09:12:55.88 15.5psi after pressurize
@09:12:55.90 Archive.in :compress
@09:12:55.97 Archive.seek :empty
@09:13:08.35 Archive.in :default
@09:13:08.40 Archive.hold
@09:13:08.68 <PROCESSING> Recovered 825uL lysate @16.0psi
@09:13:08.69 Air.in :halfSpeed
@09:13:08.76 Air.seek :fullVent
@09:13:11.01 Puck Top Vented, bottom @6.2psi
@09:13:11.02 Handoff.select :waste
@09:13:11.14 delay 5 seconds
@09:13:16.22 Handoff open to waste, bottom @0.0psi
@09:13:16.23 Air.in :default
@09:13:16.30 Air.seek :evac
@09:13:17.74 Lysis2.in :DAmix
@09:13:17.86 Lysis1.in :DAmix
@09:13:18.26 <Lysis2mixer> Lysis2.seek 1480uL
@09:13:18.31 <PROCESSING> Lysis1.seek 575uL
@09:13:53.35 delay 5 seconds
@09:13:58.37 Handoff.select SPR
@09:13:58.49 SPR.command \"start 2 14\"
@09:14:00.53 delay 10 seconds
@09:14:10.55 SPR.command \"start 1 0\"
@09:14:13.30 Waiting up to 4:00 for SPR to enter state 1
@09:16:15.67 SPR.command \"hold_on\"
@09:16:17.58 SPR Dilution 1 of 1 -- Running Experiment 1
@09:16:18.00 <Lysis2mixer> Lysis2.seek 1180uL
@09:16:18.04 <PROCESSING> Lysis1.seek 275uL
@09:17:00.04 volL1 = 300.0 and volL2 = 300.0
@09:17:00.05 delay 15 seconds
@09:17:15.07 SPR.command \"hold_off\"
@09:17:17.73 Waiting up to 38:00 for SPR to enter state 7
@09:44:00.34 SPR Analysis has Completed
@09:44:00.36 Handoff.select :waste
@09:44:01.27 <SPRlogger> Ended log /var/log/esp/spr21apr2308h.spr
@09:44:01.28 <PROCESSING> SPR.command \"stats_off\"
@09:44:02.58 <LRAUV> -> Cmd.stop
@09:44:02.59 Archive.stop
@09:44:02.71 <closeHandoff> Handoff.select :close
@09:44:02.84 <LRAUV> Gate.power :heat,:OFF
@09:44:02.86 Gate.power :cartridge,:OFF
@09:44:02.94 Carousel.seek 25
@09:44:03.12 Plunge.seek :locked
@09:44:04.00 PinPort.in :unlocking
@09:44:04.06 PinPort.seek :clear
@09:44:04.62 <Archive> Archive.in :unlocking
@09:44:04.75 Archive.seek :clear
@09:44:04.78 <Air> Air.in :unlocking
@09:44:04.93 Air.seek :clear
@09:44:05.00 <Lysis1> Lysis1.in :unlocking
@09:44:05.13 <Lysis2> Lysis2.in :unlocking
@09:44:05.17 <Lysis1> Lysis1.seek :clear
@09:44:05.26 <Lysis2> Lysis2.seek :clear
@09:44:08.77 <LRAUV> Archive.coast
@09:44:08.81 Air.coast
@09:44:08.86 Lysis1.coast
@09:44:08.91 Lysis2.coast
@09:44:08.96 Plunge.coast
@09:44:09.00 SamplePump.coast
@09:44:09.05 Toroid.coast
@09:44:09.10 Carousel.coast
@09:44:09.15 PinPort.coast
@09:44:09.33 Gate.power :motor,:OFF
@09:44:09.35 Gate.power :core,:OFF
@09:44:09.37 Gate.power :analytic1,:OFF
@09:44:09.39 Gate.power :analytic2,:OFF
@09:44:09.41 Gate.power :analytic3,:OFF
@09:44:09.43 Gate.power :main,:OFF
@09:44:09.45 Safely stopped and ready to power off
##-- file closed 2021-04-23T18:51:51.688Z

##-- file opened 2021-04-23T18:51:51.690Z
@11:51:51.50 -> showStatus
@11:51:53.52 -> Cmd.loadCartridge -6
@11:51:53.66 Gate.power :main,:ON,limit to 5000mA
@11:51:53.67 Gate.power :analytic1,:ON,limit to 5000mA
@11:51:53.69 Gate.power :analytic2,:ON,limit to 5000mA
@11:51:53.72 Gate.power :analytic3,:ON,limit to 5000mA
@11:51:53.85 Powering core bus
@11:51:56.13 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@11:51:56.26 <LRAUV> Archive.configure :default
@11:51:56.38 Air.configure :default
@11:51:56.48 Lysis1.configure :default
@11:51:56.58 Lysis2.configure :default
@11:51:56.70 Plunge.configure :default
@11:51:56.83 SamplePump.configure :default
@11:51:56.98 ExtraPump.configure :default
@11:51:57.08 Toroid.configure :default
@11:51:57.21 Carousel.configure :default
@11:51:57.33 Pressure.configure :default
@11:51:57.44 PinPort.configure :default
@11:51:57.51 All elf controllers running firmware version 4.741
@11:51:57.54 Gate.power :motor,:ON,limit to 5000mA
@11:51:58.06 <Archive_home> Archive.in :default
@11:51:58.33 Archive.home.jog 0
@11:51:58.51 <Toroid_home> Toroid.in :default
@11:51:58.59 <Plunge_home> Plunge.in :default
@11:51:58.62 <Lysis1_home> Lysis1.in :default
@11:51:58.66 <Air_home> Air.in :default
@11:51:58.69 <PinPort_home> PinPort.in :default
@11:51:58.73 <Lysis2_home> Lysis2.in :default
@11:51:58.95 <Plunge_home> Plunge.home.jog 0
@11:51:59.00 <Lysis1_home> Lysis1.home.jog 0
@11:51:59.02 <Air_home> Air.home.jog 0
@11:51:59.05 <Toroid_home> Toroid.home.jog 0
@11:51:59.07 <PinPort_home> PinPort.home.jog 0
@11:51:59.09 <Lysis2_home> Lysis2.home.jog 0
@11:51:59.49 <Archive_home> Archive.in :homing
@11:51:59.77 <Toroid_home> Toroid.in :homing
@11:51:59.81 <Plunge_home> Plunge.in :homing
@11:51:59.84 <Air_home> Air.in :homing
@11:51:59.88 <PinPort_home> PinPort.in :homing
@11:51:59.91 <Lysis1_home> Lysis1.in :homing
@11:51:59.95 <Archive_home> Archive.jog 151
@11:51:59.99 <Lysis2_home> Lysis2.in :homing
@11:52:00.18 <Toroid_home> Toroid.jog 601
@11:52:00.22 <PinPort_home> PinPort.jog -106
@11:52:00.26 <Plunge_home> Plunge.jog 1575
@11:52:00.29 <Air_home> Air.jog 172
@11:52:00.32 <Lysis1_home> Lysis1.jog 1419
@11:52:00.35 <Lysis2_home> Lysis2.jog 1430
@11:52:00.59 <Archive_home> Archive.setRawPosition! 50
@11:52:00.64 Archive.seek :home
@11:52:00.72 <PinPort_home> PinPort.setRawPosition! 0
@11:52:00.76 PinPort.seek :home
@11:52:00.86 PinPort.in :default
@11:52:00.96 <Air_home> Air.setRawPosition! 50
@11:52:00.98 <Archive_home> Archive.in :default
@11:52:01.03 <Air_home> Air.seek :home
@11:52:01.36 Air.in :default
@11:52:01.66 <Plunge_home> Plunge.setRawPosition! 50
@11:52:01.69 <Lysis2_home> Lysis2.setRawPosition! 50
@11:52:01.71 <Lysis1_home> Lysis1.setRawPosition! 50
@11:52:01.81 <Plunge_home> Plunge.seek :home
@11:52:01.85 <Lysis2_home> Lysis2.seek :home
@11:52:01.89 <Lysis1_home> Lysis1.seek :home
@11:52:02.19 <Plunge_home> Plunge.in :default
@11:52:02.29 <Lysis2_home> Lysis2.in :default
@11:52:02.38 <Lysis1_home> Lysis1.in :default
@11:52:02.45 <Toroid_home> Toroid.setRawPosition! 0
@11:52:02.50 Toroid.seek :home
@11:52:02.59 Toroid.in :default
@11:52:02.77 <LRAUV> Toroid.seek :clear
@11:52:04.39 Carousel.in :default
@11:52:04.45 Carousel.home.jog 0
@11:52:04.94 Carousel.in :default
@11:52:05.00 Carousel.seek 25
@11:52:05.13 Selecting Cartridge 28
@11:52:05.37 Carousel.seek 28
@11:52:06.27 Lysis1.in :fast
@11:52:06.33 Lysis1.seek :locked
@11:52:08.93 <Plunge> Plunge.seek :locked
@11:52:09.05 <Air> Air.in :fast
@11:52:09.11 Air.seek :locked
@11:52:11.72 <LRAUV> Powering cartridge bus
@11:52:13.85 Gate.setIR 1750,5000,0,6
@11:52:13.98 Gate.setIR 0,5000,0,6
@11:52:14.03 Gate.power :cartridge,:OFF
@11:52:14.56 Mounted archive Cartridge
@11:52:33.12 -> Cmd.startFiltering
@11:52:33.57 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@11:52:33.58 SamplePump.setPosition! 0ml
@11:52:33.62 Bypass.open
@11:52:35.11 Intake.open
@11:52:35.90 Sea 7.1m deep (25.2psia)
@11:52:35.92 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@11:52:38.50 Vacuum tare is 51% of full scale
@11:52:38.62 Pressure.in :default
@11:52:38.68 Pressure.hold
@11:52:38.85 SamplePump.in :prime
@11:52:38.92 SamplePump.seek 10ml,1:21
@11:52:48.31 Pressure.coast
@11:52:48.36 SamplePump.setPosition! 0ml
@11:52:48.41 Exhaust.open
@11:52:49.84 <closeBypass> Bypass.close
@11:52:51.38 <FILTERING> Pressure.in :default
@11:52:51.44 Pressure.hold
@11:52:51.56 Sea 7.4m deep (25.7psia)
@11:52:51.69 SamplePump.in :prime
@11:52:51.75 SamplePump.seek 100ml,8:51
@11:55:01.94 Pressure.coast
@11:55:02.00 Bypass.open
@11:55:02.71 Intake.close
@11:55:03.57 Toroid.in :hold
@11:55:03.63 Toroid.seek :crack
@11:55:05.19 Toroid.seek :cartridge
@11:55:06.27 Toroid.in :default
@11:55:06.33 Toroid.seek :clear
@11:55:08.29 Pressurizing cartridge to 26psi above 24.2psia
@11:55:08.30 SamplePump.setPosition! 0ml
@11:55:08.35 Intake.open
@11:55:09.06 Bypass.close
@11:55:09.81 Exhaust.close
@11:55:10.63 Pressure.in :default
@11:55:10.69 Pressure.hold
@11:55:10.86 SamplePump.in :charge
@11:55:10.92 SamplePump.seek 2.3ml,42 seconds
@11:55:16.81 Pressure.coast
@11:55:16.86 Intake.close
@11:55:17.70 Pressurized cartridge with 0.153ml to 26.2psi
@11:55:17.72 Isolated @45.6psia (21.4psi)
@11:55:17.73 Verifying cartridge holds at least 0.75..0.85 of 45.6psia
@11:55:17.76 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@11:55:22.38 Sampling 1000.0ml
@11:55:22.40 SamplePump.setPosition! 0ml
@11:55:22.45 Exhaust.open
@11:55:23.14 Intake.open
@11:55:23.98 Pressure.in :default
@11:55:24.45 Pressure.hold
@11:55:24.57 Sea 7.1m deep (25.2psia)
@11:55:24.70 SamplePump.in :sample
@11:55:24.78 SamplePump.seek 1000ml,1:23:53
@11:55:27.02 <sampling> Cartridge 28 filtering 1/1000ml at 0.33ml/s with 23.0psi vacuum 5.0m deep (22.0psia)
@11:56:27.02 Cartridge 28 filtering 20/1000ml at 0.34ml/s with 26.7psi vacuum 1.9m deep (17.5psia)
@11:57:27.02 Cartridge 28 filtering 38/1000ml at 0.33ml/s with 20.1psi vacuum 4.2m deep (21.0psia)
@11:58:27.02 Cartridge 28 filtering 56/1000ml at 0.26ml/s with 21.5psi vacuum 4.3m deep (21.0psia)
@11:59:27.02 Cartridge 28 filtering 74/1000ml at 0.32ml/s with 27.7psi vacuum
@12:00:27.02 Cartridge 28 filtering 91/1000ml at 0.31ml/s with 22.3psi vacuum 5.2m deep (22.3psia)
@12:01:27.02 Cartridge 28 filtering 109/1000ml at 0.25ml/s with 20.3psi vacuum 3.1m deep (19.3psia)
@12:02:27.02 Cartridge 28 filtering 126/1000ml at 0.24ml/s with 20.6psi vacuum 1.6m deep (17.1psia)
@12:03:27.02 Cartridge 28 filtering 142/1000ml at 0.24ml/s with 20.7psi vacuum 4.4m deep (21.1psia)
@12:04:27.02 Cartridge 28 filtering 159/1000ml at 0.29ml/s with 24.1psi vacuum
@12:05:27.02 Cartridge 28 filtering 175/1000ml at 0.23ml/s with 23.3psi vacuum 2.7m deep (18.6psia)
@12:06:27.02 Cartridge 28 filtering 191/1000ml at 0.22ml/s with 22.6psi vacuum 4.6m deep (21.5psia)
@12:07:27.02 Cartridge 28 filtering 207/1000ml at 0.28ml/s with 23.9psi vacuum 2.8m deep (18.8psia)
@12:08:27.02 Cartridge 28 filtering 223/1000ml at 0.21ml/s with 24.0psi vacuum 2.2m deep (17.9psia)
@12:09:27.02 Cartridge 28 filtering 238/1000ml at 0.26ml/s with 19.6psi vacuum 6.1m deep (23.7psia)
@12:10:27.02 Cartridge 28 filtering 254/1000ml at 0.26ml/s with 25.8psi vacuum 2.0m deep (17.7psia)
@12:11:27.02 Cartridge 28 filtering 269/1000ml at 0.29ml/s with 26.7psi vacuum 4.9m deep (22.0psia)
@12:12:27.02 Cartridge 28 filtering 284/1000ml at 0.24ml/s with 18.4psi vacuum 5.6m deep (22.9psia)
@12:13:27.02 Cartridge 28 filtering 299/1000ml at 0.21ml/s with 21.4psi vacuum 2.3m deep (18.1psia)
@12:14:27.02 Cartridge 28 filtering 314/1000ml at 0.26ml/s with 23.5psi vacuum 4.7m deep (21.6psia)
@12:15:27.02 Cartridge 28 filtering 328/1000ml at 0.25ml/s with 23.1psi vacuum 3.3m deep (19.5psia)
@12:16:27.02 Cartridge 28 filtering 343/1000ml at 0.27ml/s with 23.2psi vacuum 3.2m deep (19.4psia)
@12:17:27.02 Cartridge 28 filtering 357/1000ml at 0.27ml/s with 23.0psi vacuum 5.6m deep (23.0psia)
@12:18:27.02 Cartridge 28 filtering 371/1000ml at 0.21ml/s with 21.6psi vacuum 2.0m deep (17.6psia)
@12:19:27.02 Cartridge 28 filtering 385/1000ml at 0.22ml/s with 20.9psi vacuum 3.7m deep (20.2psia)
@12:20:27.02 Cartridge 28 filtering 399/1000ml at 0.21ml/s with 26.8psi vacuum 4.9m deep (21.9psia)
@12:21:27.02 Cartridge 28 filtering 413/1000ml at 0.20ml/s with 22.4psi vacuum
@12:22:27.02 Cartridge 28 filtering 426/1000ml at 0.20ml/s with 26.6psi vacuum 3.7m deep (20.2psia)
@12:23:27.02 Cartridge 28 filtering 440/1000ml at 0.23ml/s with 24.6psi vacuum 2.8m deep (18.9psia)
@12:24:27.02 Cartridge 28 filtering 453/1000ml at 0.23ml/s with 25.2psi vacuum 3.0m deep (19.2psia)
@12:25:27.02 Cartridge 28 filtering 466/1000ml at 0.20ml/s with 24.7psi vacuum 4.0m deep (20.7psia)
@12:26:27.02 Cartridge 28 filtering 480/1000ml at 0.22ml/s with 23.7psi vacuum 2.6m deep (18.5psia)
@12:27:27.02 Cartridge 28 filtering 493/1000ml at 0.20ml/s with 25.3psi vacuum 3.2m deep (19.4psia)
@12:28:27.02 Cartridge 28 filtering 505/1000ml at 0.24ml/s with 24.2psi vacuum 4.6m deep (21.5psia)
@12:29:27.02 Cartridge 28 filtering 518/1000ml at 0.20ml/s with 26.8psi vacuum 3.1m deep (19.2psia)
@12:30:27.02 Cartridge 28 filtering 531/1000ml at 0.20ml/s with 27.9psi vacuum 3.2m deep (19.4psia)
@12:31:27.02 Cartridge 28 filtering 544/1000ml at 0.22ml/s with 24.8psi vacuum 1.6m deep (17.1psia)
@12:32:27.02 Cartridge 28 filtering 556/1000ml at 0.20ml/s with 26.7psi vacuum 4.0m deep (20.5psia)
@12:33:27.02 Cartridge 28 filtering 569/1000ml at 0.20ml/s with 26.8psi vacuum 4.1m deep (20.8psia)
@12:34:27.02 Cartridge 28 filtering 581/1000ml at 0.20ml/s with 28.1psi vacuum
@12:35:27.02 Cartridge 28 filtering 594/1000ml at 0.21ml/s with 27.6psi vacuum 4.6m deep (21.5psia)
@12:36:27.02 Cartridge 28 filtering 606/1000ml at 0.20ml/s with 24.8psi vacuum 3.9m deep (20.4psia)
@12:37:27.02 Cartridge 28 filtering 618/1000ml at 0.20ml/s with 28.1psi vacuum 2.5m deep (18.4psia)
@12:38:27.02 Cartridge 28 filtering 631/1000ml at 0.20ml/s with 24.3psi vacuum 6.1m deep (23.8psia)
@12:39:27.02 Cartridge 28 filtering 643/1000ml at 0.20ml/s with 26.1psi vacuum 1.7m deep (17.2psia)
@12:39:57.91 <FILTERING> Flow averaged 0.24ml/s over 44:34
@12:39:57.94 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi
@12:39:58.52 Relieved excess vacuum in .6 seconds
@12:39:58.54 SamplePump.seek 1000ml,29:46
@12:40:19.63 Flow averaged 0.20ml/s over 22 seconds
@12:40:19.66 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi
@12:40:20.23 Relieved excess vacuum in .6 seconds
@12:40:20.26 SamplePump.seek 1000ml,29:25
@12:40:27.02 <sampling> Cartridge 28 filtering 655/1000ml at 0.20ml/s with 24.5psi vacuum 4.5m deep (21.3psia)
@12:41:27.02 Cartridge 28 filtering 667/1000ml at 0.21ml/s with 20.8psi vacuum 5.2m deep (22.4psia)
@12:41:39.78 <FILTERING> Flow averaged 0.20ml/s over 1:20
@12:41:39.81 Waiting up to 16 seconds for vacuum to reduce from 29.3 to 18.0psi
@12:41:40.39 Relieved excess vacuum in .6 seconds
@12:41:40.41 SamplePump.seek 1000ml,28:04
@12:42:20.99 Flow averaged 0.20ml/s over 41 seconds
@12:42:21.02 Waiting up to 17 seconds for vacuum to reduce from 29.7 to 18.0psi
@12:42:21.59 Relieved excess vacuum in .6 seconds
@12:42:21.61 SamplePump.seek 1000ml,27:23
@12:42:27.01 <sampling> Cartridge 28 filtering 679/1000ml at 0.20ml/s with 25.8psi vacuum 1.8m deep (17.4psia)
@12:42:32.50 <FILTERING> Flow averaged 0.20ml/s over 11 seconds
@12:42:32.52 <closeIntake> Intake.close
@12:42:32.56 <closeExhaust> Exhaust.close
@12:42:32.76 <FILTERING> Toroid.in :hold
@12:42:32.83 Toroid.seek :bypass
@12:42:34.46 Toroid.in :default
@12:42:34.52 Toroid.seek :clear
@12:42:36.13 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@12:42:36.14 Pressure.coast
@12:42:36.33 Sampled  679.8ml
@12:42:38.20 <LRAUV> -> Cmd.startProcessing
@12:42:38.63 <PROCESSING> Plunge.seek :reagent
@12:42:39.46 Sample loop closed. Ready to Deliver Reagents
@12:42:39.47 Archive.seek :sealed
@12:42:43.73 Archive.seek :evac
@12:42:57.66 delay 1:00
@12:43:57.69 Archive.seek :clear
@12:44:15.39 RNAlater soak for 10:00
@12:54:15.42 Archive.seek :tVent
@12:54:18.00 delay 1 second
@12:54:19.02 Archive.seek :evac
@12:54:34.64 delay 10 seconds
@12:54:44.67 Sample preserved and evacuated
@12:54:45.83 <LRAUV> -> Cmd.stop
@12:54:45.96 Carousel.seek 28
@12:54:46.14 Plunge.seek :locked
@12:54:47.08 <Archive> Archive.in :unlocking
@12:54:47.20 Archive.seek :clear
@12:54:47.26 <Air> Air.in :unlocking
@12:54:47.39 Air.seek :clear
@12:54:47.43 <Lysis1> Lysis1.in :unlocking
@12:54:47.58 Lysis1.seek :clear
@12:54:51.25 <LRAUV> Archive.coast
@12:54:51.30 Air.coast
@12:54:51.35 Lysis1.coast
@12:54:51.40 Lysis2.coast
@12:54:51.45 Plunge.coast
@12:54:51.50 SamplePump.coast
@12:54:51.55 Toroid.coast
@12:54:51.60 Carousel.coast
@12:54:51.65 PinPort.coast
@12:54:51.83 Gate.power :motor,:OFF
@12:54:51.85 Gate.power :core,:OFF
@12:54:51.87 Gate.power :analytic1,:OFF
@12:54:51.89 Gate.power :analytic2,:OFF
@12:54:51.91 Gate.power :analytic3,:OFF
@12:54:51.93 Gate.power :main,:OFF
@12:54:51.95 Safely stopped and ready to power off
##-- file closed 2021-04-23T20:06:56.760Z

##-- file opened 2021-04-23T20:06:56.764Z
@13:06:57.30 -> showStatus
@13:06:59.32 -> Cmd.loadCartridge -9
@13:06:59.46 Gate.power :main,:ON,limit to 5000mA
@13:06:59.48 Gate.power :analytic1,:ON,limit to 5000mA
@13:06:59.50 Gate.power :analytic2,:ON,limit to 5000mA
@13:06:59.52 Gate.power :analytic3,:ON,limit to 5000mA
@13:06:59.66 Powering core bus
@13:07:01.93 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@13:07:02.06 <LRAUV> Archive.configure :default
@13:07:02.18 Air.configure :default
@13:07:02.28 Lysis1.configure :default
@13:07:02.39 Lysis2.configure :default
@13:07:02.51 Plunge.configure :default
@13:07:02.63 SamplePump.configure :default
@13:07:02.78 ExtraPump.configure :default
@13:07:02.89 Toroid.configure :default
@13:07:03.01 Carousel.configure :default
@13:07:03.14 Pressure.configure :default
@13:07:03.24 PinPort.configure :default
@13:07:03.64 All elf controllers running firmware version 4.741
@13:07:03.69 Gate.power :motor,:ON,limit to 5000mA
@13:07:04.00 <Archive_home> Archive.in :default
@13:07:04.10 <Air_home> Air.in :default
@13:07:04.26 Air.home.jog 0
@13:07:04.28 <Archive_home> Archive.home.jog 0
@13:07:04.45 <Toroid_home> Toroid.in :default
@13:07:04.53 <Plunge_home> Plunge.in :default
@13:07:04.57 <Lysis1_home> Lysis1.in :default
@13:07:04.66 <Lysis2_home> Lysis2.in :default
@13:07:04.73 <PinPort_home> PinPort.in :default
@13:07:04.83 <Toroid_home> Toroid.home.jog 0
@13:07:04.91 <Lysis1_home> Lysis1.home.jog 0
@13:07:04.98 <Plunge_home> Plunge.home.jog 0
@13:07:05.00 <PinPort_home> PinPort.home.jog 0
@13:07:05.03 <Lysis2_home> Lysis2.home.jog 0
@13:07:05.31 <Air_home> Air.in :homing
@13:07:05.42 <Archive_home> Archive.in :homing
@13:07:05.66 <Toroid_home> Toroid.in :homing
@13:07:05.69 <Plunge_home> Plunge.in :homing
@13:07:05.73 <Lysis1_home> Lysis1.in :homing
@13:07:05.76 <Air_home> Air.jog 158
@13:07:05.80 <Archive_home> Archive.jog 152
@13:07:05.83 <PinPort_home> PinPort.in :homing
@13:07:05.86 <Lysis2_home> Lysis2.in :homing
@13:07:06.03 <Toroid_home> Toroid.jog 611
@13:07:06.09 <Plunge_home> Plunge.jog 1581
@13:07:06.14 <Lysis1_home> Lysis1.jog 1420
@13:07:06.18 <PinPort_home> PinPort.jog -107
@13:07:06.22 <Lysis2_home> Lysis2.jog 1430
@13:07:06.40 <Air_home> Air.setRawPosition! 50
@13:07:06.44 Air.seek :home
@13:07:06.54 <Archive_home> Archive.setRawPosition! 50
@13:07:06.56 <PinPort_home> PinPort.setRawPosition! 0
@13:07:06.62 <Archive_home> Archive.seek :home
@13:07:06.66 <PinPort_home> PinPort.seek :home
@13:07:06.80 <Air_home> Air.in :default
@13:07:06.82 <PinPort_home> PinPort.in :default
@13:07:06.97 <Archive_home> Archive.in :default
@13:07:07.41 <Lysis1_home> Lysis1.setRawPosition! 50
@13:07:07.47 Lysis1.seek :home
@13:07:07.56 <Lysis2_home> Lysis2.setRawPosition! 50
@13:07:07.58 <Plunge_home> Plunge.setRawPosition! 50
@13:07:07.67 <Lysis2_home> Lysis2.seek :home
@13:07:07.69 <Plunge_home> Plunge.seek :home
@13:07:07.83 <Lysis1_home> Lysis1.in :default
@13:07:08.02 <Plunge_home> Plunge.in :default
@13:07:08.05 <Lysis2_home> Lysis2.in :default
@13:07:08.23 <Toroid_home> Toroid.setRawPosition! 0
@13:07:08.28 Toroid.seek :home
@13:07:08.36 Toroid.in :default
@13:07:08.50 <LRAUV> Toroid.seek :clear
@13:07:10.12 Carousel.in :default
@13:07:10.18 Carousel.home.jog 0
@13:07:10.68 Carousel.in :default
@13:07:10.74 Carousel.seek 28
@13:07:10.90 Selecting Cartridge 23
@13:07:11.15 Carousel.seek 23
@13:07:12.45 Lysis1.in :fast
@13:07:12.51 Lysis1.seek :locked
@13:07:15.11 <Plunge> Plunge.seek :locked
@13:07:15.24 <Air> Air.in :fast
@13:07:15.30 Air.seek :locked
@13:07:17.90 <LRAUV> Powering cartridge bus
@13:07:20.02 Gate.setIR 1750,5000,0,6
@13:07:20.15 Gate.setIR 0,5000,0,6
@13:07:20.21 PuckHeater.configure :default
@13:07:20.25 Gate.power :heat,:ON,limit to 5000mA
@13:07:21.00 Mounted lysis Cartridge board#Q05044
@13:07:21.03 Using default puck heat loss curve for HeatingAccelInCarousel
@13:07:21.05 Ignoring this cartridge's heat loss curve
@13:07:40.13 -> Cmd.startFiltering
@13:07:40.57 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@13:07:40.59 SamplePump.setPosition! 0ml
@13:07:40.63 Bypass.open
@13:07:42.12 Intake.open
@13:07:42.90 Sea 4.6m deep (21.5psia)
@13:07:42.91 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@13:07:45.48 Vacuum tare is 51% of full scale
@13:07:45.67 Pressure.in :default
@13:07:45.73 Pressure.hold
@13:07:45.89 SamplePump.in :prime
@13:07:45.96 SamplePump.seek 10ml,1:21
@13:07:55.34 Pressure.coast
@13:07:55.39 SamplePump.setPosition! 0ml
@13:07:55.44 Exhaust.open
@13:07:56.87 <closeBypass> Bypass.close
@13:07:58.45 <FILTERING> Pressure.in :default
@13:07:58.51 Pressure.hold
@13:07:58.62 Sea 5.3m deep (22.6psia)
@13:07:58.75 SamplePump.in :prime
@13:07:58.82 SamplePump.seek 100ml,8:51
@13:10:08.93 Pressure.coast
@13:10:08.99 Bypass.open
@13:10:09.72 Intake.close
@13:10:10.56 Toroid.in :hold
@13:10:10.62 Toroid.seek :crack
@13:10:12.17 Toroid.seek :cartridge
@13:10:13.27 Toroid.in :default
@13:10:13.33 Toroid.seek :clear
@13:10:15.29 Pressurizing cartridge to 26psi above 20.8psia
@13:10:15.30 SamplePump.setPosition! 0ml
@13:10:15.34 Intake.open
@13:10:16.08 Bypass.close
@13:10:16.78 Exhaust.close
@13:10:17.61 Pressure.in :default
@13:10:17.67 Pressure.hold
@13:10:17.84 SamplePump.in :charge
@13:10:17.91 SamplePump.seek 2.3ml,42 seconds
@13:10:27.40 Pressure.coast
@13:10:27.45 Intake.close
@13:10:28.28 Pressurized cartridge with 0.401ml to 26.6psi
@13:10:28.29 Isolated @43.3psia (22.5psi)
@13:10:28.31 Verifying cartridge holds at least 0.75..0.85 of 43.3psia
@13:10:28.35 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@13:10:32.92 Cartridge pressure fell 2% to 42.6psia
@13:10:33.40 Sampling 1000.0ml
@13:10:33.42 SamplePump.setPosition! 0ml
@13:10:33.47 Exhaust.open
@13:10:34.20 Intake.open
@13:10:35.10 Pressure.in :default
@13:10:35.16 Pressure.hold
@13:10:35.28 Sea 6.1m deep (23.6psia)
@13:10:35.41 SamplePump.in :sample
@13:10:35.49 SamplePump.seek 1000ml,1:23:53
@13:10:38.13 <sampling> Cartridge 23 filtering 1/1000ml at 0.35ml/s with 23.7psi vacuum 4.7m deep (21.6psia)
@13:11:38.13 Cartridge 23 filtering 19/1000ml at 0.30ml/s with 22.8psi vacuum 3.4m deep (19.7psia)
@13:12:38.13 Cartridge 23 filtering 38/1000ml at 0.29ml/s with 23.6psi vacuum 2.3m deep (18.1psia)
@13:13:38.12 Cartridge 23 filtering 55/1000ml at 0.32ml/s with 25.8psi vacuum 4.1m deep (20.7psia)
@13:14:38.13 Cartridge 23 filtering 73/1000ml at 0.27ml/s with 26.3psi vacuum 4.3m deep (21.1psia)
@13:15:38.13 Cartridge 23 filtering 90/1000ml at 0.29ml/s with 27.5psi vacuum
@13:16:38.13 Cartridge 23 filtering 106/1000ml at 0.27ml/s with 27.4psi vacuum 2.9m deep (19.0psia)
@13:17:38.13 Cartridge 23 filtering 123/1000ml at 0.30ml/s with 21.3psi vacuum 5.5m deep (22.9psia)
@13:18:38.13 Cartridge 23 filtering 139/1000ml at 0.24ml/s with 26.3psi vacuum
@13:19:38.13 Cartridge 23 filtering 155/1000ml at 0.30ml/s with 22.3psi vacuum 5.4m deep (22.7psia)
@13:20:38.13 Cartridge 23 filtering 171/1000ml at 0.30ml/s with 25.1psi vacuum 4.5m deep (21.3psia)
@13:21:38.13 Cartridge 23 filtering 186/1000ml at 0.29ml/s with 24.6psi vacuum 3.1m deep (19.2psia)
@13:22:38.13 Cartridge 23 filtering 202/1000ml at 0.24ml/s with 23.3psi vacuum 4.9m deep (21.9psia)
@13:23:38.13 Cartridge 23 filtering 217/1000ml at 0.23ml/s with 27.1psi vacuum 2.5m deep (18.4psia)
@13:24:38.13 Cartridge 23 filtering 232/1000ml at 0.27ml/s with 26.7psi vacuum 2.9m deep (19.0psia)
@13:25:38.13 Cartridge 23 filtering 246/1000ml at 0.23ml/s with 22.5psi vacuum 5.2m deep (22.4psia)
@13:26:38.13 Cartridge 23 filtering 261/1000ml at 0.25ml/s with 26.9psi vacuum 2.4m deep (18.3psia)
@13:27:38.13 Cartridge 23 filtering 275/1000ml at 0.27ml/s with 23.2psi vacuum 3.9m deep (20.4psia)
@13:28:38.13 Cartridge 23 filtering 289/1000ml at 0.21ml/s with 22.7psi vacuum 4.9m deep (21.9psia)
@13:29:38.13 Cartridge 23 filtering 303/1000ml at 0.20ml/s with 24.9psi vacuum
@13:30:38.13 Cartridge 23 filtering 317/1000ml at 0.21ml/s with 22.4psi vacuum 3.7m deep (20.2psia)
@13:31:38.13 Cartridge 23 filtering 331/1000ml at 0.21ml/s with 23.0psi vacuum 4.5m deep (21.3psia)
@13:32:38.13 Cartridge 23 filtering 344/1000ml at 0.22ml/s with 22.1psi vacuum 1.7m deep (17.2psia)
@13:33:38.13 Cartridge 23 filtering 357/1000ml at 0.21ml/s with 23.6psi vacuum 4.9m deep (21.9psia)
@13:34:38.13 Cartridge 23 filtering 371/1000ml at 0.24ml/s with 24.9psi vacuum 4.0m deep (20.6psia)
@13:35:38.13 Cartridge 23 filtering 384/1000ml at 0.24ml/s with 23.8psi vacuum 1.9m deep (17.5psia)
@13:36:38.13 Cartridge 23 filtering 397/1000ml at 0.21ml/s with 24.0psi vacuum 4.8m deep (21.8psia)
@13:37:38.13 Cartridge 23 filtering 410/1000ml at 0.21ml/s with 26.8psi vacuum 3.3m deep (19.6psia)
@13:38:38.13 Cartridge 23 filtering 422/1000ml at 0.23ml/s with 25.8psi vacuum 2.2m deep (17.9psia)
@13:39:38.13 Cartridge 23 filtering 435/1000ml at 0.21ml/s with 23.9psi vacuum 6.0m deep (23.6psia)
@13:40:38.13 Cartridge 23 filtering 448/1000ml at 0.22ml/s with 24.0psi vacuum 2.7m deep (18.7psia)
@13:41:38.13 Cartridge 23 filtering 460/1000ml at 0.22ml/s with 23.3psi vacuum 3.8m deep (20.4psia)
@13:42:38.13 Cartridge 23 filtering 472/1000ml at 0.20ml/s with 24.5psi vacuum 5.2m deep (22.3psia)
@13:43:38.13 Cartridge 23 filtering 485/1000ml at 0.20ml/s with 27.8psi vacuum
@13:44:38.13 Cartridge 23 filtering 497/1000ml at 0.20ml/s with 27.7psi vacuum 3.2m deep (19.4psia)
@13:45:38.13 Cartridge 23 filtering 509/1000ml at 0.20ml/s with 26.4psi vacuum 3.4m deep (19.8psia)
@13:46:38.13 Cartridge 23 filtering 521/1000ml at 0.20ml/s with 27.6psi vacuum
@13:47:00.74 <FILTERING> Flow averaged 0.24ml/s over 36:26
@13:47:00.77 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi
@13:47:01.59 Relieved excess vacuum in .9 seconds
@13:47:01.62 SamplePump.seek 1000ml,40:03
@13:47:07.71 Flow estimate reduced to 0.19ml/s
@13:47:07.74 <closeIntake> Intake.close
@13:47:07.79 <closeExhaust> Exhaust.close
@13:47:07.96 <FILTERING> Toroid.in :hold
@13:47:08.03 Toroid.seek :bypass
@13:47:09.66 Toroid.in :default
@13:47:09.72 Toroid.seek :clear
@13:47:11.33 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@13:47:11.34 Pressure.coast
@13:47:11.53 Sampled  527.0ml
@13:47:13.72 <LRAUV> -> Cmd.startProcessing
@13:47:15.15 <PROCESSING> SPR.command \"read_sequences\"
@13:47:15.59 SPR.command \"stats_on\"
@13:47:15.63 SPR.command \"start 0 1\"
@13:47:17.64 <SPRlogger> Begun log /var/log/esp/spr21apr2313h.spr
@13:47:18.44 <PROCESSING> Handoff.select :waste
@13:47:25.12 Syringe tare is 20% of full scale
@13:47:25.14 PinPort.in :locking
@13:47:25.21 PinPort.seek :locked
@13:47:25.68 <posArchive> Archive.in :fast
@13:47:25.73 <posLyS2> Lysis2.in :fast
@13:47:25.77 <PROCESSING> Lysis1.seek 1350uL
@13:47:25.90 <posLyS2> Lysis2.seek :full
@13:47:25.92 <posArchive> Archive.seek :full
@13:47:27.99 <PROCESSING> Plunge.seek :reagent
@13:47:28.89 Evacuating puck with filter bubble point of 50psi
@13:47:28.91 PinPort.seek :locked
@13:47:28.99 Handoff.select :waste
@13:47:35.58 Air syringe pumping up to 1 stroke to reach 8psi
@13:47:35.59 Air stroke #1 of 1
@13:47:35.67 Air.in :pressurize
@13:47:35.73 Air.seek :pressurized
@13:47:46.98 -0.2psi after pressurize
@13:47:46.99 Air.in :compress
@13:47:47.06 Air.seek :evac
@13:47:51.17 -0.0psi after compress
@13:48:03.74 -0.2psi after compress (stable)
@13:48:03.75 Air.in :default
@13:48:03.82 Air.hold
@13:48:03.99 at -0.2psi Opened to waste
@13:48:04.01 Handoff.select :blocked
@13:48:04.14 Archive syringe pumping up to 5 strokes to reach 50psi
@13:48:04.15 Archive stroke #1 of 5
@13:48:04.24 Archive.in :pressurize
@13:48:04.31 Archive.seek :pressurized
@13:48:17.05 1.0psi after pressurize
@13:48:17.06 Archive.in :compress
@13:48:17.14 Archive.seek :empty
@13:48:33.01 17.4psi after compress
@13:49:20.09 44.5psi after compress (stable)
@13:49:20.11 Archive.in :gulp
@13:49:20.18 Archive.seek :topVent
@13:49:27.38 46.5psi after gulp
@13:49:39.95 47.2psi after gulp (stable)
@13:49:39.96 Archive stroke #2 of 5
@13:49:40.05 Archive.in :pressurize
@13:49:40.11 Archive.seek :pressurized
@13:49:52.84 47.4psi after pressurize
@13:49:52.85 Archive.in :compress
@13:49:52.93 Archive.seek :empty
@13:50:07.98 Reached 50.3 of 50.0psi after pumping 3443uL (1.99 strokes)
@13:50:07.99 Archive.in :default
@13:50:08.06 Archive.hold
@13:50:08.12 Handoff.select :waste
@13:50:18.83 delay 1:00
@13:51:18.85 Handoff.select :blocked
@13:51:41.54 Handoff.select :waste
@13:51:41.65 2.2psi at evacEnd
@13:51:41.66 Handoff.select :blocked
@13:51:41.78 Archive.in :fast
@13:51:41.85 Archive.seek :topVent
@13:51:55.54 Stable @3.2psi after Handoff blocked
@13:51:55.58 Archive syringe pumping up to 5 strokes to reach 33psi
@13:51:55.60 Archive stroke #1 of 5
@13:51:55.69 Archive.in :pressurize
@13:51:55.75 Archive.seek :pressurized
@13:52:08.47 4.9psi after pressurize
@13:52:08.48 Archive.in :compress
@13:52:08.56 Archive.seek :empty
@13:52:24.47 7.2psi after compress
@13:53:01.13 Reached 33.2 of 33.2psi after pumping 1730uL (1.00 strokes)
@13:53:01.14 Archive.in :default
@13:53:01.22 Archive.hold
@13:53:01.32 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position
@13:53:01.35 Handoff.select :waste
@13:53:14.03 delay 1:00
@13:54:14.06 Handoff.select :blocked
@13:54:42.29 Pressure too High, open to waste again (8.1psi)
@13:54:42.30 Handoff.select :waste
@13:54:42.43 delay 1:00
@13:55:42.45 Handoff.select :blocked
@13:56:21.69 Pressure too High, open to waste again (8.0psi)
@13:56:21.70 Handoff.select :waste
@13:56:21.83 delay 1:00
@13:57:21.85 Handoff.select :blocked
@13:58:10.03 Handoff.select :waste
@13:58:10.15 7.1psi at evacEnd
@13:58:10.16 Air.seek :fullVent
@13:58:11.61 Archive.in :fast
@13:58:11.67 Archive.seek :topVent
@13:58:18.81 Seawater evacuated, Presure above/below filter released. Lysis begins
@13:58:18.90 Lysis1.in :slow
@13:58:18.96 Lysis1.seek 1050uL
@13:58:48.98 delay 5 seconds
@13:58:54.00 Handoff.select :blocked
@13:58:54.17 Lysis1.seek 450uL
@13:59:54.13 delay 3 seconds
@13:59:57.20 Air.in to mix into 1.37806 parts Lysis1
@13:59:57.76 <Airmixer> Air.seek :evac
@13:59:57.80 <PROCESSING> Lysis1.seek 300uL
@14:00:12.91 Lysis1.seek :empty
@14:00:43.00 Lysis buffer delivered @25.3psi
@14:00:43.01 Polling Can every 2:00
@14:00:43.56 PuckHeater.configure :default #ambient 11.5C
@14:00:43.60 PuckHeater.seek 80.2C
@14:01:55.22 PuckHeater.hold 2:00,80.2C
@14:03:55.31 Polling Can every 2:00
@14:03:55.85 PuckHeater.configure :default #ambient 11.6C
@14:03:55.90 PuckHeater.hold 30 seconds,79.2C
@14:04:26.12 Polling Can every 2:00
@14:04:26.67 PuckHeater.configure :default #ambient 11.6C
@14:04:26.71 PuckHeater.hold 30 seconds,78.7C
@14:04:56.93 Polling Can every 2:00
@14:04:57.48 PuckHeater.configure :default #ambient 11.6C
@14:04:57.53 PuckHeater.hold 10:00,78.1C
@14:14:58.16 Lysis complete. Syringe pressure @31.9psi
@14:14:58.17 Handoff.select :blocked
@14:14:58.21 Archive syringe pumping up to 3 strokes to reach 40psi
@14:14:58.22 Archive stroke #1 of 3
@14:14:58.31 Archive.in :pressurize
@14:14:58.37 Archive.seek :pressurized
@14:15:11.10 31.6psi after pressurize
@14:15:11.13 Archive.in :compress
@14:15:11.21 Archive.seek :empty
@14:15:21.46 Reached 40.8 of 40.0psi after pumping 1610uL (0.93 strokes)
@14:15:21.47 Archive.in :default
@14:15:21.55 Archive.hold
@14:15:21.63 delay 2 seconds
@14:15:23.65 Archive.in :fast
@14:15:23.71 Archive.seek :full
@14:15:30.50 Lysis1.seek 138uL
@14:15:44.37 delay 1 second
@14:15:45.40 <fillArchive> Archive.seek :full
@14:15:45.42 <PROCESSING> Lysis1.seek :empty
@14:15:59.43 Initial air pressure @55.3psi
@14:15:59.50 Lysis1.in :extract
@14:15:59.57 Lysis1.seek 600uL,nil
@14:16:01.32 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@14:16:01.33 Archive stroke #1 of 6
@14:16:01.43 Archive.in :pressurize
@14:16:01.50 Archive.seek :pressurized
@14:16:14.26 15.8psi after pressurize
@14:16:14.28 Archive.in :compress
@14:16:14.34 Archive.seek :empty
@14:16:30.27 15.8psi after compress
@14:16:42.84 15.8psi after compress (stable)
@14:16:48.44 Archive.in :gulp
@14:16:48.50 Archive.seek :topVent
@14:16:55.71 15.8psi after gulp
@14:17:08.28 15.9psi after gulp (stable)
@14:17:08.29 Archive stroke #2 of 6
@14:17:08.44 Archive.in :pressurize
@14:17:08.51 Archive.seek :pressurized
@14:17:21.24 15.8psi after pressurize
@14:17:21.25 Archive.in :compress
@14:17:21.32 Archive.seek :empty
@14:17:36.09 Archive.in :default
@14:17:36.14 Archive.hold
@14:17:36.36 <PROCESSING> Initial 600uL lysate @18.5psi
@14:17:36.38 Handoff.select :blocked
@14:17:36.46 Initial air pressure @19.8psi
@14:17:36.53 Lysis1.seek 825uL,nil
@14:17:37.74 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@14:17:37.76 Archive stroke #1 of 6
@14:17:37.84 Archive.in :compress
@14:17:37.91 Archive.seek :empty
@14:17:38.47 Reached 17.5 of 17.0psi after pumping 1720uL (0.99 strokes)
@14:17:38.49 Archive.in :default
@14:17:38.56 Archive.hold
@14:17:38.74 Slide::Error in pump -- Slide::Error while holding Archive Syringe (ArS) in position
@14:17:39.42 Archive syringe pumping up to 6 strokes to reach 17psi
@14:17:39.43 Archive stroke #1 of 6
@14:17:39.52 Archive.in :compress
@14:17:39.58 Archive.seek :empty
@14:17:40.18 Reached 17.3 of 17.0psi after pumping 1726uL (1.00 strokes)
@14:17:40.20 Archive.in :default
@14:17:40.26 Archive.hold
@14:17:40.99 Archive syringe pumping up to 6 strokes to reach 17psi
@14:17:41.00 Archive stroke #1 of 6
@14:17:41.09 Archive.in :compress
@14:17:41.16 Archive.seek :empty
@14:17:41.59 16.8psi after compress
@14:17:41.82 Reached 17.0 of 17.0psi after pumping 1730uL (1.00 strokes)
@14:17:41.83 Archive.in :default
@14:17:41.92 Archive.hold
@14:17:42.67 Archive syringe pumping up to 6 strokes to reach 17psi
@14:17:42.68 Archive stroke #1 of 6
@14:17:42.77 Archive.in :compress
@14:17:42.83 Archive.seek :empty
@14:17:43.01 15.7psi after compress
@14:17:43.47 Reached 16.9 of 17.0psi after pumping 1730uL (1.00 strokes)
@14:17:43.48 Archive.in :default
@14:17:43.55 Archive.hold
@14:17:44.29 Archive syringe pumping up to 6 strokes to reach 17psi
@14:17:44.30 Archive stroke #1 of 6
@14:17:44.41 Archive.in :compress
@14:17:44.47 Archive.seek :empty
@14:17:44.71 16.0psi after compress
@14:17:52.07 Archive.in :default
@14:17:52.12 Archive.hold
@14:17:52.30 <PROCESSING> Recovered 825uL lysate @15.8psi
@14:17:52.31 Air.in :halfSpeed
@14:17:52.38 Air.seek :fullVent
@14:17:54.62 Puck Top Vented, bottom @2.5psi
@14:17:54.63 Handoff.select :waste
@14:17:54.76 delay 5 seconds
@14:17:59.83 Handoff open to waste, bottom @0.0psi
@14:17:59.85 Air.in :default
@14:17:59.92 Air.seek :evac
@14:18:01.36 Lysis2.in :DAmix
@14:18:01.49 Lysis1.in :DAmix
@14:18:02.00 <Lysis2mixer> Lysis2.seek 1480uL
@14:18:02.05 <PROCESSING> Lysis1.seek 575uL
@14:18:37.09 delay 5 seconds
@14:18:42.11 Handoff.select SPR
@14:18:42.22 SPR.command \"start 2 14\"
@14:18:43.61 delay 10 seconds
@14:18:53.63 SPR.command \"start 1 0\"
@14:18:56.84 Waiting up to 4:00 for SPR to enter state 1
@14:20:59.22 SPR.command \"hold_on\"
@14:21:00.63 SPR Dilution 1 of 1 -- Running Experiment 1
@14:21:01.07 <Lysis2mixer> Lysis2.seek 1180uL
@14:21:01.13 <PROCESSING> Lysis1.seek 275uL
@14:21:43.14 volL1 = 300.0 and volL2 = 300.0
@14:21:43.15 delay 15 seconds
@14:21:58.18 SPR.command \"hold_off\"
@14:22:01.29 Waiting up to 38:00 for SPR to enter state 7
@14:48:44.83 SPR Analysis has Completed
@14:48:44.85 Handoff.select :waste
@14:48:45.76 <SPRlogger> Ended log /var/log/esp/spr21apr2313h.spr
@14:48:45.77 <PROCESSING> SPR.command \"stats_off\"
@14:48:47.27 <LRAUV> -> Cmd.stop
@14:48:47.36 <closeHandoff> Handoff.select :close
@14:48:47.48 <LRAUV> Gate.power :heat,:OFF
@14:48:47.50 Gate.power :cartridge,:OFF
@14:48:47.58 Carousel.seek 23
@14:48:47.75 Plunge.seek :locked
@14:48:48.63 PinPort.in :unlocking
@14:48:48.69 PinPort.seek :clear
@14:48:49.23 <Archive> Archive.in :unlocking
@14:48:49.35 Archive.seek :clear
@14:48:49.42 <Air> Air.in :unlocking
@14:48:49.55 Air.seek :clear
@14:48:49.61 <Lysis1> Lysis1.in :unlocking
@14:48:49.74 <Lysis2> Lysis2.in :unlocking
@14:48:49.78 <Lysis1> Lysis1.seek :clear
@14:48:49.86 <Lysis2> Lysis2.seek :clear
@14:48:53.41 <LRAUV> Archive.coast
@14:48:53.46 Air.coast
@14:48:53.50 Lysis1.coast
@14:48:53.55 Lysis2.coast
@14:48:53.60 Plunge.coast
@14:48:53.65 SamplePump.coast
@14:48:53.70 Toroid.coast
@14:48:53.74 Carousel.coast
@14:48:53.79 PinPort.coast
@14:48:53.97 Gate.power :motor,:OFF
@14:48:53.99 Gate.power :core,:OFF
@14:48:54.01 Gate.power :analytic1,:OFF
@14:48:54.03 Gate.power :analytic2,:OFF
@14:48:54.05 Gate.power :analytic3,:OFF
@14:48:54.07 Gate.power :main,:OFF
@14:48:54.09 Safely stopped and ready to power off
##-- file closed 2021-04-23T23:55:51.853Z

##-- file opened 2021-04-23T23:55:51.855Z
@16:55:51.84 -> showStatus
@16:55:53.87 -> Cmd.loadCartridge -6
@16:55:54.01 Gate.power :main,:ON,limit to 5000mA
@16:55:54.02 Gate.power :analytic1,:ON,limit to 5000mA
@16:55:54.04 Gate.power :analytic2,:ON,limit to 5000mA
@16:55:54.07 Gate.power :analytic3,:ON,limit to 5000mA
@16:55:54.20 Powering core bus
@16:55:56.48 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@16:55:56.61 <LRAUV> Archive.configure :default
@16:55:56.73 Air.configure :default
@16:55:56.83 Lysis1.configure :default
@16:55:56.94 Lysis2.configure :default
@16:55:57.06 Plunge.configure :default
@16:55:57.19 SamplePump.configure :default
@16:55:57.33 ExtraPump.configure :default
@16:55:57.44 Toroid.configure :default
@16:55:57.56 Carousel.configure :default
@16:55:57.68 Pressure.configure :default
@16:55:58.14 PinPort.configure :default
@16:55:58.20 All elf controllers running firmware version 4.741
@16:55:58.23 Gate.power :motor,:ON,limit to 5000mA
@16:55:58.47 <Archive_home> Archive.in :default
@16:55:58.70 Archive.home.jog 0
@16:55:58.90 <PinPort_home> PinPort.in :default
@16:55:58.94 <Toroid_home> Toroid.in :default
@16:55:58.98 <Lysis2_home> Lysis2.in :default
@16:55:59.01 <Air_home> Air.in :default
@16:55:59.05 <Lysis1_home> Lysis1.in :default
@16:55:59.11 <Plunge_home> Plunge.in :default
@16:55:59.32 <PinPort_home> PinPort.home.jog 0
@16:55:59.36 <Lysis2_home> Lysis2.home.jog 0
@16:55:59.39 <Toroid_home> Toroid.home.jog 0
@16:55:59.41 <Air_home> Air.home.jog 0
@16:55:59.45 <Plunge_home> Plunge.home.jog 0
@16:55:59.48 <Lysis1_home> Lysis1.home.jog 0
@16:55:59.93 <Archive_home> Archive.in :homing
@16:56:00.12 <Toroid_home> Toroid.in :homing
@16:56:00.16 <Plunge_home> Plunge.in :homing
@16:56:00.19 <Air_home> Air.in :homing
@16:56:00.24 <PinPort_home> PinPort.in :homing
@16:56:00.27 <Lysis2_home> Lysis2.in :homing
@16:56:00.31 <Archive_home> Archive.jog 174
@16:56:00.34 <Lysis1_home> Lysis1.in :homing
@16:56:00.53 <Plunge_home> Plunge.jog 1584
@16:56:00.58 <Toroid_home> Toroid.jog 609
@16:56:00.61 <Air_home> Air.jog 172
@16:56:00.63 <PinPort_home> PinPort.jog -107
@16:56:00.66 <Lysis2_home> Lysis2.jog 151
@16:56:00.69 <Lysis1_home> Lysis1.jog 1414
@16:56:00.94 <Archive_home> Archive.setRawPosition! 50
@16:56:00.99 Archive.seek :home
@16:56:01.08 <PinPort_home> PinPort.setRawPosition! 0
@16:56:01.12 PinPort.seek :home
@16:56:01.23 PinPort.in :default
@16:56:01.32 <Lysis2_home> Lysis2.setRawPosition! 50
@16:56:01.34 <Archive_home> Archive.in :default
@16:56:01.38 <Air_home> Air.setRawPosition! 50
@16:56:01.44 <Lysis2_home> Lysis2.seek :home
@16:56:01.55 <Air_home> Air.seek :home
@16:56:01.76 <Lysis2_home> Lysis2.in :default
@16:56:01.85 <Air_home> Air.in :default
@16:56:01.93 <Plunge_home> Plunge.setRawPosition! 50
@16:56:02.00 Plunge.seek :home
@16:56:02.08 <Lysis1_home> Lysis1.setRawPosition! 50
@16:56:02.13 Lysis1.seek :home
@16:56:02.28 <Plunge_home> Plunge.in :default
@16:56:02.41 <Lysis1_home> Lysis1.in :default
@16:56:02.80 <Toroid_home> Toroid.setRawPosition! 0
@16:56:02.84 Toroid.seek :home
@16:56:02.93 Toroid.in :default
@16:56:03.06 <LRAUV> Toroid.seek :clear
@16:56:04.68 Carousel.in :default
@16:56:04.74 Carousel.home.jog 0
@16:56:05.23 Carousel.in :default
@16:56:05.29 Carousel.seek 23
@16:56:05.42 Selecting Cartridge 26
@16:56:05.66 Carousel.seek 26
@16:56:06.55 Lysis1.in :fast
@16:56:06.61 Lysis1.seek :locked
@16:56:09.21 <Plunge> Plunge.seek :locked
@16:56:09.33 <Air> Air.in :fast
@16:56:09.39 Air.seek :locked
@16:56:11.99 <LRAUV> Powering cartridge bus
@16:56:14.11 Gate.setIR 1750,5000,0,6
@16:56:14.24 Gate.setIR 0,5000,0,6
@16:56:14.29 Gate.power :cartridge,:OFF
@16:56:14.82 Mounted archive Cartridge
@16:56:33.51 -> Cmd.startFiltering
@16:56:33.97 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@16:56:33.98 SamplePump.setPosition! 0ml
@16:56:34.03 Bypass.open
@16:56:35.50 Intake.open
@16:56:36.28 Sea 6.1m deep (23.7psia)
@16:56:36.29 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@16:56:38.87 Vacuum tare is 51% of full scale
@16:56:39.00 Pressure.in :default
@16:56:39.06 Pressure.hold
@16:56:39.29 SamplePump.in :prime
@16:56:39.35 SamplePump.seek 10ml,1:21
@16:56:48.74 Pressure.coast
@16:56:48.79 SamplePump.setPosition! 0ml
@16:56:48.84 Exhaust.open
@16:56:50.29 <closeBypass> Bypass.close
@16:56:51.84 <FILTERING> Pressure.in :default
@16:56:51.90 Pressure.hold
@16:56:52.00 Sea 5.1m deep (22.3psia)
@16:56:52.14 SamplePump.in :prime
@16:56:52.20 SamplePump.seek 100ml,8:51
@16:59:02.22 Pressure.coast
@16:59:02.28 Bypass.open
@16:59:03.01 Intake.close
@16:59:03.85 Toroid.in :hold
@16:59:03.91 Toroid.seek :crack
@16:59:05.47 Toroid.seek :cartridge
@16:59:06.56 Toroid.in :default
@16:59:06.62 Toroid.seek :clear
@16:59:08.59 Pressurizing cartridge to 26psi above 23.5psia
@16:59:08.60 SamplePump.setPosition! 0ml
@16:59:08.65 Intake.open
@16:59:09.38 Bypass.close
@16:59:10.13 Exhaust.close
@16:59:10.95 Pressure.in :default
@16:59:11.01 Pressure.hold
@16:59:11.17 SamplePump.in :charge
@16:59:11.24 SamplePump.seek 2.3ml,42 seconds
@16:59:17.21 Pressure.coast
@16:59:17.26 Intake.close
@16:59:18.12 Pressurized cartridge with 0.157ml to 26.5psi
@16:59:18.13 Isolated @43.2psia (19.7psi)
@16:59:18.15 Verifying cartridge holds at least 0.75..0.85 of 43.2psia
@16:59:18.18 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@16:59:22.80 Sampling 1000.0ml
@16:59:22.82 SamplePump.setPosition! 0ml
@16:59:22.87 Exhaust.open
@16:59:23.59 Intake.open
@16:59:24.89 Pressure.in :default
@16:59:24.95 Pressure.hold
@16:59:25.07 Sea 5.3m deep (22.5psia)
@16:59:25.20 SamplePump.in :sample
@16:59:25.27 SamplePump.seek 1000ml,1:23:53
@16:59:27.91 <sampling> Cartridge 26 filtering 1/1000ml at 0.29ml/s with 28.9psi vacuum
@17:00:27.92 Cartridge 26 filtering 19/1000ml at 0.30ml/s with 20.0psi vacuum 3.3m deep (19.5psia)
@17:01:27.92 Cartridge 26 filtering 38/1000ml at 0.32ml/s with 22.6psi vacuum 3.6m deep (20.0psia)
@17:02:27.92 Cartridge 26 filtering 56/1000ml at 0.33ml/s with 21.8psi vacuum 2.9m deep (19.0psia)
@17:03:27.92 Cartridge 26 filtering 73/1000ml at 0.31ml/s with 24.1psi vacuum 2.4m deep (18.2psia)
@17:04:27.92 Cartridge 26 filtering 91/1000ml at 0.31ml/s with 24.4psi vacuum 4.7m deep (21.6psia)
@17:05:27.92 Cartridge 26 filtering 108/1000ml at 0.33ml/s with 24.2psi vacuum 3.0m deep (19.1psia)
@17:06:27.92 Cartridge 26 filtering 124/1000ml at 0.22ml/s with 25.2psi vacuum 3.5m deep (19.9psia)
@17:07:27.92 Cartridge 26 filtering 141/1000ml at 0.31ml/s with 22.7psi vacuum 4.4m deep (21.2psia)
@17:08:27.92 Cartridge 26 filtering 157/1000ml at 0.29ml/s with 23.6psi vacuum 2.9m deep (19.0psia)
@17:09:27.92 Cartridge 26 filtering 174/1000ml at 0.29ml/s with 21.2psi vacuum 5.5m deep (22.9psia)
@17:10:27.92 Cartridge 26 filtering 189/1000ml at 0.22ml/s with 22.9psi vacuum 2.6m deep (18.6psia)
@17:11:27.92 Cartridge 26 filtering 205/1000ml at 0.23ml/s with 27.2psi vacuum 3.2m deep (19.4psia)
@17:12:27.92 Cartridge 26 filtering 221/1000ml at 0.24ml/s with 19.8psi vacuum 4.5m deep (21.4psia)
@17:13:27.92 Cartridge 26 filtering 236/1000ml at 0.23ml/s with 27.7psi vacuum
@17:14:27.92 Cartridge 26 filtering 251/1000ml at 0.20ml/s with 26.5psi vacuum 3.3m deep (19.6psia)
@17:15:27.92 Cartridge 26 filtering 267/1000ml at 0.27ml/s with 21.4psi vacuum 3.3m deep (19.6psia)
@17:16:27.92 Cartridge 26 filtering 281/1000ml at 0.27ml/s with 21.2psi vacuum 3.6m deep (20.0psia)
@17:17:27.92 Cartridge 26 filtering 296/1000ml at 0.21ml/s with 21.5psi vacuum 5.6m deep (23.0psia)
@17:18:27.92 Cartridge 26 filtering 311/1000ml at 0.21ml/s with 22.1psi vacuum 1.7m deep (17.2psia)
@17:19:27.98 Cartridge 26 filtering 325/1000ml at 0.27ml/s with 23.8psi vacuum 5.8m deep (23.3psia)
@17:20:27.92 Cartridge 26 filtering 340/1000ml at 0.26ml/s with 27.6psi vacuum 3.7m deep (20.1psia)
@17:21:27.92 Cartridge 26 filtering 354/1000ml at 0.25ml/s with 24.6psi vacuum 2.5m deep (18.4psia)
@17:22:27.92 Cartridge 26 filtering 368/1000ml at 0.26ml/s with 22.3psi vacuum 6.3m deep (23.9psia)
@17:23:27.92 Cartridge 26 filtering 382/1000ml at 0.25ml/s with 24.4psi vacuum 3.0m deep (19.2psia)
@17:24:27.92 Cartridge 26 filtering 395/1000ml at 0.22ml/s with 26.1psi vacuum 3.8m deep (20.3psia)
@17:25:27.92 Cartridge 26 filtering 409/1000ml at 0.22ml/s with 25.2psi vacuum 4.4m deep (21.2psia)
@17:26:27.92 Cartridge 26 filtering 423/1000ml at 0.23ml/s with 23.3psi vacuum
@17:27:27.92 Cartridge 26 filtering 436/1000ml at 0.22ml/s with 26.3psi vacuum 3.1m deep (19.3psia)
@17:28:27.92 Cartridge 26 filtering 450/1000ml at 0.24ml/s with 27.0psi vacuum 4.7m deep (21.7psia)
@17:29:27.92 Cartridge 26 filtering 463/1000ml at 0.22ml/s with 23.8psi vacuum
@17:30:27.92 Cartridge 26 filtering 476/1000ml at 0.22ml/s with 23.7psi vacuum 2.8m deep (18.8psia)
@17:31:27.92 Cartridge 26 filtering 489/1000ml at 0.22ml/s with 22.9psi vacuum 4.8m deep (21.8psia)
@17:32:27.92 Cartridge 26 filtering 502/1000ml at 0.20ml/s with 25.1psi vacuum
@17:33:27.92 Cartridge 26 filtering 515/1000ml at 0.21ml/s with 23.6psi vacuum 4.3m deep (21.1psia)
@17:34:27.92 Cartridge 26 filtering 528/1000ml at 0.21ml/s with 23.0psi vacuum 4.6m deep (21.5psia)
@17:35:27.92 Cartridge 26 filtering 540/1000ml at 0.21ml/s with 23.6psi vacuum 2.1m deep (17.7psia)
@17:36:27.92 Cartridge 26 filtering 553/1000ml at 0.20ml/s with 27.7psi vacuum 4.7m deep (21.6psia)
@17:37:27.92 Cartridge 26 filtering 565/1000ml at 0.20ml/s with 27.1psi vacuum 2.2m deep (17.9psia)
@17:38:27.92 Cartridge 26 filtering 578/1000ml at 0.20ml/s with 26.2psi vacuum 1.7m deep (17.3psia)
@17:39:27.92 Cartridge 26 filtering 590/1000ml at 0.20ml/s with 30.5psi vacuum 4.4m deep (21.2psia)
@17:40:27.91 Cartridge 26 filtering 603/1000ml at 0.22ml/s with 25.0psi vacuum 2.6m deep (18.5psia)
@17:41:27.92 Cartridge 26 filtering 615/1000ml at 0.20ml/s with 25.8psi vacuum 3.4m deep (19.8psia)
@17:42:27.91 Cartridge 26 filtering 627/1000ml at 0.22ml/s with 24.7psi vacuum 5.4m deep (22.7psia)
@17:43:27.92 Cartridge 26 filtering 640/1000ml at 0.20ml/s with 30.0psi vacuum
@17:44:27.91 Cartridge 26 filtering 652/1000ml at 0.20ml/s with 28.9psi vacuum 3.3m deep (19.6psia)
@17:44:40.13 <FILTERING> Flow averaged 0.24ml/s over 45:15
@17:44:40.16 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi
@17:44:40.74 Relieved excess vacuum in .6 seconds
@17:44:40.76 SamplePump.seek 1000ml,29:20
@17:44:52.64 Flow averaged 0.20ml/s over 12 seconds
@17:44:52.66 <closeIntake> Intake.close
@17:44:52.70 <closeExhaust> Exhaust.close
@17:44:52.88 <FILTERING> Toroid.in :hold
@17:44:52.95 Toroid.seek :bypass
@17:44:54.58 Toroid.in :default
@17:44:54.64 Toroid.seek :clear
@17:44:56.26 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@17:44:56.27 Pressure.coast
@17:44:56.46 Sampled  656.7ml
@17:44:58.39 <LRAUV> -> Cmd.startProcessing
@17:44:58.77 <PROCESSING> Plunge.seek :reagent
@17:44:59.60 Sample loop closed. Ready to Deliver Reagents
@17:44:59.61 Archive.seek :sealed
@17:45:03.87 Archive.seek :evac
@17:45:17.81 delay 1:00
@17:46:17.83 Archive.seek :clear
@17:46:35.55 RNAlater soak for 10:00
@17:56:35.58 Archive.seek :tVent
@17:56:38.15 delay 1 second
@17:56:39.17 Archive.seek :evac
@17:56:54.79 delay 10 seconds
@17:57:04.82 Sample preserved and evacuated
@17:57:06.02 <LRAUV> -> Cmd.stop
@17:57:06.16 Carousel.seek 26
@17:57:06.33 Plunge.seek :locked
@17:57:07.28 <Archive> Archive.in :unlocking
@17:57:07.39 Archive.seek :clear
@17:57:07.47 <Air> Air.in :unlocking
@17:57:07.59 <Lysis1> Lysis1.in :unlocking
@17:57:07.63 <Air> Air.seek :clear
@17:57:07.79 <Lysis1> Lysis1.seek :clear
@17:57:11.40 <LRAUV> Archive.coast
@17:57:11.46 Air.coast
@17:57:11.50 Lysis1.coast
@17:57:11.55 Lysis2.coast
@17:57:11.60 Plunge.coast
@17:57:11.65 SamplePump.coast
@17:57:11.70 Toroid.coast
@17:57:11.76 Carousel.coast
@17:57:11.81 PinPort.coast
@17:57:11.98 Gate.power :motor,:OFF
@17:57:12.00 Gate.power :core,:OFF
@17:57:12.02 Gate.power :analytic1,:OFF
@17:57:12.04 Gate.power :analytic2,:OFF
@17:57:12.06 Gate.power :analytic3,:OFF
@17:57:12.08 Gate.power :main,:OFF
@17:57:12.10 Safely stopped and ready to power off
##-- file closed 2021-04-24T01:08:43.746Z

##-- file opened 2021-04-24T01:08:43.747Z
@18:08:43.80 -> showStatus
@18:08:45.83 -> Cmd.loadCartridge -9
@18:08:45.97 Gate.power :main,:ON,limit to 5000mA
@18:08:45.99 Gate.power :analytic1,:ON,limit to 5000mA
@18:08:46.01 Gate.power :analytic2,:ON,limit to 5000mA
@18:08:46.03 Gate.power :analytic3,:ON,limit to 5000mA
@18:08:46.16 Powering core bus
@18:08:48.44 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@18:08:48.57 <LRAUV> Archive.configure :default
@18:08:48.69 Air.configure :default
@18:08:48.79 Lysis1.configure :default
@18:08:48.90 Lysis2.configure :default
@18:08:49.02 Plunge.configure :default
@18:08:49.15 SamplePump.configure :default
@18:08:49.30 ExtraPump.configure :default
@18:08:49.40 Toroid.configure :default
@18:08:49.53 Carousel.configure :default
@18:08:50.01 Pressure.configure :default
@18:08:50.11 PinPort.configure :default
@18:08:50.17 All elf controllers running firmware version 4.741
@18:08:50.20 Gate.power :motor,:ON,limit to 5000mA
@18:08:50.47 <Archive_home> Archive.in :default
@18:08:50.71 Archive.home.jog 0
@18:08:50.88 <PinPort_home> PinPort.in :default
@18:08:50.92 <Toroid_home> Toroid.in :default
@18:08:50.96 <Lysis2_home> Lysis2.in :default
@18:08:50.99 <Air_home> Air.in :default
@18:08:51.03 <Lysis1_home> Lysis1.in :default
@18:08:51.09 <Plunge_home> Plunge.in :default
@18:08:51.29 <PinPort_home> PinPort.home.jog 0
@18:08:51.32 <Toroid_home> Toroid.home.jog 0
@18:08:51.34 <Lysis2_home> Lysis2.home.jog 0
@18:08:51.37 <Air_home> Air.home.jog 0
@18:08:51.40 <Lysis1_home> Lysis1.home.jog 0
@18:08:51.43 <Plunge_home> Plunge.home.jog 0
@18:08:51.88 <Archive_home> Archive.in :homing
@18:08:52.13 <Toroid_home> Toroid.in :homing
@18:08:52.15 <Plunge_home> Plunge.in :homing
@18:08:52.18 <Air_home> Air.in :homing
@18:08:52.23 <PinPort_home> PinPort.in :homing
@18:08:52.26 <Lysis2_home> Lysis2.in :homing
@18:08:52.30 <Archive_home> Archive.jog 154
@18:08:52.34 <Lysis1_home> Lysis1.in :homing
@18:08:52.52 <Plunge_home> Plunge.jog 1577
@18:08:52.54 <Toroid_home> Toroid.jog 616
@18:08:52.59 <Air_home> Air.jog 151
@18:08:52.62 <PinPort_home> PinPort.jog -107
@18:08:52.65 <Lysis2_home> Lysis2.jog 1428
@18:08:52.72 <Lysis1_home> Lysis1.jog 1415
@18:08:52.92 <Archive_home> Archive.setRawPosition! 50
@18:08:52.97 Archive.seek :home
@18:08:53.06 <PinPort_home> PinPort.setRawPosition! 0
@18:08:53.10 PinPort.seek :home
@18:08:53.20 PinPort.in :default
@18:08:53.24 <Air_home> Air.setRawPosition! 50
@18:08:53.30 Air.seek :home
@18:08:53.40 <Archive_home> Archive.in :default
@18:08:53.60 <Air_home> Air.in :default
@18:08:53.89 <Plunge_home> Plunge.setRawPosition! 50
@18:08:53.94 Plunge.seek :home
@18:08:54.04 <Lysis2_home> Lysis2.setRawPosition! 50
@18:08:54.06 <Lysis1_home> Lysis1.setRawPosition! 50
@18:08:54.14 <Lysis2_home> Lysis2.seek :home
@18:08:54.17 <Lysis1_home> Lysis1.seek :home
@18:08:54.32 <Plunge_home> Plunge.in :default
@18:08:54.48 <Lysis2_home> Lysis2.in :default
@18:08:54.56 <Lysis1_home> Lysis1.in :default
@18:08:54.78 <Toroid_home> Toroid.setRawPosition! 0
@18:08:54.83 Toroid.seek :home
@18:08:54.92 Toroid.in :default
@18:08:55.05 <LRAUV> Toroid.seek :clear
@18:08:56.68 Carousel.in :default
@18:08:56.74 Carousel.home.jog 0
@18:08:57.24 Carousel.in :default
@18:08:57.30 Carousel.seek 26
@18:08:57.44 Selecting Cartridge 21
@18:08:57.69 Carousel.seek 21
@18:08:58.98 Lysis1.in :fast
@18:08:59.04 Lysis1.seek :locked
@18:09:01.64 <Plunge> Plunge.seek :locked
@18:09:01.77 <Air> Air.in :fast
@18:09:01.83 Air.seek :locked
@18:09:04.44 <LRAUV> Powering cartridge bus
@18:09:06.56 Gate.setIR 1750,5000,0,6
@18:09:06.69 Gate.setIR 0,5000,0,6
@18:09:06.76 PuckHeater.configure :default
@18:09:06.81 Gate.power :heat,:ON,limit to 5000mA
@18:09:07.49 Mounted lysis Cartridge board#Q05037
@18:09:07.52 Using default puck heat loss curve for HeatingAccelInCarousel
@18:09:26.23 -> Cmd.startFiltering
@18:09:26.68 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@18:09:26.70 SamplePump.setPosition! 0ml
@18:09:26.74 Bypass.open
@18:09:28.25 Intake.open
@18:09:29.05 Sea 5.1m deep (22.3psia)
@18:09:29.07 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@18:09:31.65 Vacuum tare is 51% of full scale
@18:09:31.78 Pressure.in :default
@18:09:31.83 Pressure.hold
@18:09:32.00 SamplePump.in :prime
@18:09:32.07 SamplePump.seek 10ml,1:21
@18:09:41.46 Pressure.coast
@18:09:41.51 SamplePump.setPosition! 0ml
@18:09:41.56 Exhaust.open
@18:09:42.96 <closeBypass> Bypass.close
@18:09:44.55 <FILTERING> Pressure.in :default
@18:09:44.61 Pressure.hold
@18:09:44.72 Sea 6.7m deep (24.6psia)
@18:09:44.85 SamplePump.in :prime
@18:09:44.91 SamplePump.seek 100ml,8:51
@18:11:55.08 Pressure.coast
@18:11:55.14 Bypass.open
@18:11:55.87 Intake.close
@18:11:56.71 Toroid.in :hold
@18:11:56.77 Toroid.seek :crack
@18:11:58.32 Toroid.seek :cartridge
@18:11:59.41 Toroid.in :default
@18:11:59.47 Toroid.seek :clear
@18:12:01.43 Pressurizing cartridge to 26psi above 21.9psia
@18:12:01.44 SamplePump.setPosition! 0ml
@18:12:01.48 Intake.open
@18:12:02.21 Bypass.close
@18:12:02.95 Exhaust.close
@18:12:03.75 Pressure.in :default
@18:12:03.81 Pressure.hold
@18:12:03.98 SamplePump.in :charge
@18:12:04.04 SamplePump.seek 2.3ml,42 seconds
@18:12:13.86 Pressure.coast
@18:12:13.91 Intake.close
@18:12:14.74 Pressurized cartridge with 0.429ml to 26.6psi
@18:12:14.76 Isolated @45.2psia (23.3psi)
@18:12:14.77 Verifying cartridge holds at least 0.75..0.85 of 45.2psia
@18:12:14.81 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@18:12:19.43 Sampling 1000.0ml
@18:12:19.45 SamplePump.setPosition! 0ml
@18:12:19.50 Exhaust.open
@18:12:20.22 Intake.open
@18:12:21.08 Pressure.in :default
@18:12:21.14 Pressure.hold
@18:12:21.26 Sea 6.9m deep (24.9psia)
@18:12:21.39 SamplePump.in :sample
@18:12:21.46 SamplePump.seek 1000ml,1:23:53
@18:12:24.13 <sampling> Cartridge 21 filtering 1/1000ml at 0.33ml/s with 30.2psi vacuum 3.0m deep (19.1psia)
@18:13:24.11 Cartridge 21 filtering 19/1000ml at 0.25ml/s with 26.3psi vacuum 1.9m deep (17.4psia)
@18:14:24.12 Cartridge 21 filtering 37/1000ml at 0.32ml/s with 26.1psi vacuum 1.7m deep (17.1psia)
@18:15:24.12 Cartridge 21 filtering 54/1000ml at 0.26ml/s with 29.3psi vacuum 3.3m deep (19.6psia)
@18:16:24.12 Cartridge 21 filtering 71/1000ml at 0.24ml/s with 23.9psi vacuum
@18:17:24.12 Cartridge 21 filtering 88/1000ml at 0.22ml/s with 24.6psi vacuum 2.4m deep (18.2psia)
@18:18:24.12 Cartridge 21 filtering 104/1000ml at 0.23ml/s with 26.3psi vacuum 3.4m deep (19.8psia)
@18:19:24.12 Cartridge 21 filtering 121/1000ml at 0.23ml/s with 20.0psi vacuum
@18:20:24.12 Cartridge 21 filtering 136/1000ml at 0.28ml/s with 22.0psi vacuum 4.9m deep (21.9psia)
@18:21:24.12 Cartridge 21 filtering 152/1000ml at 0.29ml/s with 23.8psi vacuum 4.5m deep (21.4psia)
@18:22:24.12 Cartridge 21 filtering 167/1000ml at 0.26ml/s with 21.5psi vacuum 1.9m deep (17.4psia)
@18:23:24.12 Cartridge 21 filtering 183/1000ml at 0.23ml/s with 27.4psi vacuum 3.9m deep (20.4psia)
@18:24:24.12 Cartridge 21 filtering 197/1000ml at 0.28ml/s with 23.1psi vacuum 4.6m deep (21.6psia)
@18:25:24.12 Cartridge 21 filtering 212/1000ml at 0.22ml/s with 27.9psi vacuum
@18:26:24.12 Cartridge 21 filtering 226/1000ml at 0.25ml/s with 24.2psi vacuum 3.9m deep (20.4psia)
@18:27:24.12 Cartridge 21 filtering 241/1000ml at 0.22ml/s with 21.1psi vacuum 4.8m deep (21.8psia)
@18:28:24.12 Cartridge 21 filtering 255/1000ml at 0.24ml/s with 24.0psi vacuum
@18:29:24.12 Cartridge 21 filtering 269/1000ml at 0.20ml/s with 24.5psi vacuum 3.9m deep (20.4psia)
@18:30:24.12 Cartridge 21 filtering 282/1000ml at 0.20ml/s with 23.6psi vacuum 4.6m deep (21.5psia)
@18:31:24.12 Cartridge 21 filtering 296/1000ml at 0.24ml/s with 21.1psi vacuum 2.1m deep (17.7psia)
@18:32:24.12 Cartridge 21 filtering 309/1000ml at 0.23ml/s with 25.0psi vacuum 4.4m deep (21.2psia)
@18:33:24.12 Cartridge 21 filtering 322/1000ml at 0.21ml/s with 27.1psi vacuum 3.6m deep (20.1psia)
@18:34:24.12 Cartridge 21 filtering 335/1000ml at 0.23ml/s with 27.2psi vacuum
@18:35:24.12 Cartridge 21 filtering 348/1000ml at 0.20ml/s with 23.4psi vacuum 4.4m deep (21.2psia)
@18:36:24.12 Cartridge 21 filtering 361/1000ml at 0.20ml/s with 26.9psi vacuum 2.8m deep (18.8psia)
@18:37:24.12 Cartridge 21 filtering 374/1000ml at 0.23ml/s with 23.8psi vacuum 3.1m deep (19.3psia)
@18:38:24.12 Cartridge 21 filtering 386/1000ml at 0.20ml/s with 26.1psi vacuum 5.5m deep (22.8psia)
@18:39:24.12 Cartridge 21 filtering 399/1000ml at 0.22ml/s with 23.2psi vacuum 3.9m deep (20.5psia)
@18:40:24.12 Cartridge 21 filtering 411/1000ml at 0.20ml/s with 26.6psi vacuum
@18:41:24.12 Cartridge 21 filtering 423/1000ml at 0.20ml/s with 24.5psi vacuum 4.9m deep (21.9psia)
@18:42:07.34 <FILTERING> Flow averaged 0.24ml/s over 29:46
@18:42:07.37 Waiting up to 8 seconds for vacuum to reduce from 26.0 to 18.0psi
@18:42:07.94 Relieved excess vacuum in .6 seconds
@18:42:07.97 SamplePump.seek 1000ml,47:53
@18:42:24.12 <sampling> Cartridge 21 filtering 435/1000ml at 0.20ml/s with 28.9psi vacuum 3.8m deep (20.3psia)
@18:43:24.12 Cartridge 21 filtering 447/1000ml at 0.20ml/s with 29.2psi vacuum
@18:43:26.03 <FILTERING> Flow averaged 0.20ml/s over 1:19
@18:43:26.06 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@18:43:26.64 Relieved excess vacuum in .6 seconds
@18:43:26.66 SamplePump.seek 1000ml,46:34
@18:43:58.42 Flow averaged 0.20ml/s over 32 seconds
@18:43:58.45 Waiting up to 13 seconds for vacuum to reduce from 27.8 to 18.0psi
@18:43:59.01 Relieved excess vacuum in .6 seconds
@18:43:59.04 SamplePump.seek 1000ml,46:02
@18:44:03.63 Flow estimate reduced to 0.16ml/s
@18:44:03.64 <closeIntake> Intake.close
@18:44:03.69 <closeExhaust> Exhaust.close
@18:44:03.89 <FILTERING> Toroid.in :hold
@18:44:03.96 Toroid.seek :bypass
@18:44:05.59 Toroid.in :default
@18:44:05.65 Toroid.seek :clear
@18:44:07.27 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@18:44:07.28 Pressure.coast
@18:44:07.47 Sampled  455.0ml
@18:44:09.74 <LRAUV> -> Cmd.startProcessing
@18:44:11.17 <PROCESSING> SPR.command \"read_sequences\"
@18:44:11.73 SPR.command \"stats_on\"
@18:44:11.77 SPR.command \"start 0 1\"
@18:44:13.78 <SPRlogger> Begun log /var/log/esp/spr21apr2318h.spr
@18:44:14.58 <PROCESSING> Handoff.select :waste
@18:44:21.26 Syringe tare is 20% of full scale
@18:44:21.28 PinPort.in :locking
@18:44:21.35 PinPort.seek :locked
@18:44:21.83 <posArchive> Archive.in :fast
@18:44:21.87 <posLyS2> Lysis2.in :fast
@18:44:21.93 <PROCESSING> Lysis1.seek 1350uL
@18:44:22.02 <posArchive> Archive.seek :full
@18:44:22.07 <posLyS2> Lysis2.seek :full
@18:44:24.18 <PROCESSING> Plunge.seek :reagent
@18:44:25.08 Evacuating puck with filter bubble point of 50psi
@18:44:25.10 PinPort.seek :locked
@18:44:25.19 Handoff.select :waste
@18:44:32.27 Air syringe pumping up to 1 stroke to reach 8psi
@18:44:32.28 Air stroke #1 of 1
@18:44:32.36 Air.in :pressurize
@18:44:32.42 Air.seek :pressurized
@18:44:43.68 -0.0psi after pressurize
@18:44:43.69 Air.in :compress
@18:44:43.76 Air.seek :evac
@18:44:47.85 0.6psi after compress
@18:45:00.42 0.3psi after compress (stable)
@18:45:00.45 Air.in :default
@18:45:00.52 Air.hold
@18:45:00.69 at 0.4psi Opened to waste
@18:45:00.70 Handoff.select :blocked
@18:45:00.84 Archive syringe pumping up to 5 strokes to reach 50psi
@18:45:00.85 Archive stroke #1 of 5
@18:45:00.93 Archive.in :pressurize
@18:45:00.99 Archive.seek :pressurized
@18:45:13.75 10.3psi after pressurize
@18:45:13.77 Archive.in :compress
@18:45:13.84 Archive.seek :empty
@18:45:29.74 35.3psi after compress
@18:45:50.83 49.1psi after compress (stable)
@18:45:50.85 Archive.in :gulp
@18:45:50.92 Archive.seek :topVent
@18:45:51.65 Reached 50.1 of 50.0psi after pumping 1730uL (1.00 strokes)
@18:45:51.66 Archive.in :default
@18:45:51.73 Archive.hold
@18:45:52.16 Handoff.select :waste
@18:46:01.86 delay 1:00
@18:47:01.88 Handoff.select :blocked
@18:47:24.55 Handoff.select :waste
@18:47:24.67 0.0psi at evacEnd
@18:47:24.68 Handoff.select :blocked
@18:47:24.80 Archive.in :fast
@18:47:24.87 Archive.seek :topVent
@18:47:38.25 Stable @0.0psi after Handoff blocked
@18:47:38.27 Archive syringe pumping up to 5 strokes to reach 30psi
@18:47:38.29 Archive stroke #1 of 5
@18:47:38.38 Archive.in :pressurize
@18:47:38.47 Archive.seek :pressurized
@18:47:51.65 0.1psi after pressurize
@18:47:51.68 Archive.in :compress
@18:47:51.75 Archive.seek :empty
@18:48:07.65 0.2psi after compress
@18:48:20.22 0.3psi after compress (stable)
@18:48:20.24 Archive.in :gulp
@18:48:20.31 Archive.seek :topVent
@18:48:27.51 0.7psi after gulp
@18:48:40.08 1.0psi after gulp (stable)
@18:48:40.09 Archive stroke #2 of 5
@18:48:40.18 Archive.in :pressurize
@18:48:40.25 Archive.seek :pressurized
@18:48:52.99 1.3psi after pressurize
@18:48:53.00 Archive.in :compress
@18:48:53.08 Archive.seek :empty
@18:49:09.00 2.2psi after compress
@18:49:58.45 Reached 30.1 of 30.0psi after pumping 3460uL (2.00 strokes)
@18:49:58.46 Archive.in :default
@18:49:58.53 Archive.hold
@18:49:58.60 Handoff.select :waste
@18:50:22.28 delay 1:00
@18:51:22.31 Handoff.select :blocked
@18:51:44.99 Handoff.select :waste
@18:51:45.11 2.6psi at evacEnd
@18:51:45.12 Air.seek :fullVent
@18:51:46.59 Archive.in :fast
@18:51:46.65 Archive.seek :topVent
@18:51:53.80 Seawater evacuated, Presure above/below filter released. Lysis begins
@18:51:53.88 Lysis1.in :slow
@18:51:53.94 Lysis1.seek 1050uL
@18:52:23.98 delay 5 seconds
@18:52:29.00 Handoff.select :blocked
@18:52:29.16 Lysis1.seek 450uL
@18:53:29.17 delay 3 seconds
@18:53:32.25 Air.in to mix into 1.37806 parts Lysis1
@18:53:32.78 <Airmixer> Air.seek :evac
@18:53:32.82 <PROCESSING> Lysis1.seek 300uL
@18:53:47.93 Lysis1.seek :empty
@18:54:18.04 Lysis buffer delivered @25.8psi
@18:54:18.05 Polling Can every 2:00
@18:54:18.60 PuckHeater.configure :default #ambient 11.6C
@18:54:18.64 PuckHeater.seek 80.2C
@18:55:23.91 PuckHeater.hold 2:00,80.2C
@18:57:23.54 Polling Can every 2:00
@18:57:24.08 PuckHeater.configure :default #ambient 11.7C
@18:57:24.12 PuckHeater.hold 30 seconds,79.2C
@18:57:54.22 Polling Can every 2:00
@18:57:54.76 PuckHeater.hold 30 seconds,78.6C
@18:58:24.85 Polling Can every 2:00
@18:58:25.40 PuckHeater.hold 10:00,78.1C
@19:08:23.54 Lysis complete. Syringe pressure @34.5psi
@19:08:23.55 Handoff.select :blocked
@19:08:23.59 Archive syringe pumping up to 3 strokes to reach 40psi
@19:08:23.61 Archive stroke #1 of 3
@19:08:23.70 Archive.in :pressurize
@19:08:23.76 Archive.seek :pressurized
@19:08:36.51 33.9psi after pressurize
@19:08:36.52 Archive.in :compress
@19:08:36.59 Archive.seek :empty
@19:08:45.29 Reached 41.9 of 40.0psi after pumping 1576uL (0.91 strokes)
@19:08:45.30 Archive.in :default
@19:08:45.37 Archive.hold
@19:08:45.47 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position
@19:08:45.49 delay 2 seconds
@19:08:47.51 Archive.in :fast
@19:08:47.57 Archive.seek :full
@19:08:54.24 Lysis1.seek 138uL
@19:09:08.13 delay 1 second
@19:09:09.16 <fillArchive> Archive.seek :full
@19:09:09.60 <PROCESSING> Lysis1.seek :empty
@19:09:23.57 Initial air pressure @49.8psi
@19:09:23.63 Lysis1.in :extract
@19:09:23.73 Lysis1.seek 600uL,nil
@19:09:25.44 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@19:09:25.45 Archive stroke #1 of 6
@19:09:25.55 Archive.in :pressurize
@19:09:25.62 Archive.seek :pressurized
@19:09:38.39 15.6psi after pressurize
@19:09:38.40 Archive.in :compress
@19:09:38.47 Archive.seek :empty
@19:09:54.35 15.6psi after compress
@19:09:56.16 Archive.in :default
@19:09:56.21 Archive.hold
@19:09:56.33 <PROCESSING> Initial 600uL lysate @16.9psi
@19:09:56.34 Handoff.select :blocked
@19:09:56.43 Initial air pressure @17.7psi
@19:09:56.50 Lysis1.seek 825uL,nil
@19:09:57.14 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@19:09:57.15 Archive stroke #1 of 6
@19:09:57.23 Archive.in :compress
@19:09:57.29 Archive.seek :empty
@19:09:57.46 13.3psi after compress
@19:09:58.21 Reached 17.3 of 17.0psi after pumping 1730uL (1.00 strokes)
@19:09:58.22 Archive.in :default
@19:09:58.29 Archive.hold
@19:09:59.02 Archive syringe pumping up to 6 strokes to reach 17psi
@19:09:59.03 Archive stroke #1 of 6
@19:09:59.12 Archive.in :compress
@19:09:59.19 Archive.seek :empty
@19:09:59.42 15.8psi after compress
@19:09:59.71 Reached 17.2 of 17.0psi after pumping 1730uL (1.00 strokes)
@19:09:59.72 Archive.in :default
@19:09:59.79 Archive.hold
@19:10:00.55 Archive syringe pumping up to 6 strokes to reach 17psi
@19:10:00.56 Archive stroke #1 of 6
@19:10:00.70 Archive.in :compress
@19:10:00.76 Archive.seek :empty
@19:10:00.94 16.1psi after compress
@19:10:13.52 15.8psi after compress (stable)
@19:10:18.64 Recovering 4.9uL/s
@19:10:23.70 Recovering 3.5uL/s
@19:10:24.54 Archive.in :default
@19:10:24.59 Archive.hold
@19:10:24.76 <PROCESSING> Recovered 825uL lysate @15.8psi
@19:10:24.78 Air.in :halfSpeed
@19:10:24.85 Air.seek :fullVent
@19:10:27.10 Puck Top Vented, bottom @1.9psi
@19:10:27.11 Handoff.select :waste
@19:10:27.23 delay 5 seconds
@19:10:32.29 Handoff open to waste, bottom @0.1psi
@19:10:32.30 Air.in :default
@19:10:32.37 Air.seek :evac
@19:10:33.82 Lysis2.in :DAmix
@19:10:33.92 Lysis1.in :DAmix
@19:10:34.40 <Lysis2mixer> Lysis2.seek 1480uL
@19:10:34.43 <PROCESSING> Lysis1.seek 575uL
@19:11:09.51 delay 5 seconds
@19:11:14.53 Handoff.select SPR
@19:11:14.65 SPR.command \"start 2 14\"
@19:11:15.84 delay 10 seconds
@19:11:25.86 SPR.command \"start 1 0\"
@19:11:29.10 Waiting up to 4:00 for SPR to enter state 1
@19:13:31.47 SPR.command \"hold_on\"
@19:13:32.88 SPR Dilution 1 of 1 -- Running Experiment 1
@19:13:33.32 <Lysis2mixer> Lysis2.seek 1180uL
@19:13:33.37 <PROCESSING> Lysis1.seek 275uL
@19:14:15.44 volL1 = 300.0 and volL2 = 300.0
@19:14:15.45 delay 15 seconds
@19:14:30.48 SPR.command \"hold_off\"
@19:14:33.54 Waiting up to 38:00 for SPR to enter state 7
@19:41:17.08 SPR Analysis has Completed
@19:41:17.10 Handoff.select :waste
@19:41:18.02 <SPRlogger> Ended log /var/log/esp/spr21apr2318h.spr
@19:41:18.03 <PROCESSING> SPR.command \"stats_off\"
@19:41:19.54 <LRAUV> -> Cmd.stop
@19:41:19.62 <closeHandoff> Handoff.select :close
@19:41:19.75 <LRAUV> Gate.power :heat,:OFF
@19:41:19.77 Gate.power :cartridge,:OFF
@19:41:19.85 Carousel.seek 21
@19:41:20.03 Plunge.seek :locked
@19:41:20.92 PinPort.in :unlocking
@19:41:20.98 PinPort.seek :clear
@19:41:21.54 <Archive> Archive.in :unlocking
@19:41:21.66 Archive.seek :clear
@19:41:21.70 <Air> Air.in :unlocking
@19:41:21.85 Air.seek :clear
@19:41:21.89 <Lysis1> Lysis1.in :unlocking
@19:41:22.04 Lysis1.seek :clear
@19:41:22.11 <Lysis2> Lysis2.in :unlocking
@19:41:22.18 Lysis2.seek :clear
@19:41:25.66 Slide::Error in LRAUV -- Archive Syringe (ArS) positionErr at 1054uL (sensed 1030uL)
@19:41:25.68 <LRAUV> Archive.coast
@19:41:25.73 Air.coast
@19:41:25.77 Lysis1.coast
@19:41:25.82 Lysis2.coast
@19:41:25.87 Plunge.coast
@19:41:25.91 SamplePump.coast
@19:41:25.96 Toroid.coast
@19:41:26.01 Carousel.coast
@19:41:26.06 PinPort.coast
@19:41:26.24 Gate.power :motor,:OFF
@19:41:26.26 Gate.power :core,:OFF
@19:41:26.28 Gate.power :analytic1,:OFF
@19:41:26.30 Gate.power :analytic2,:OFF
@19:41:26.32 Gate.power :analytic3,:OFF
@19:41:26.34 Gate.power :main,:OFF
@19:41:26.36 ready to power off
##-- file closed 2021-04-24T04:48:05.622Z

##-- file opened 2021-04-24T04:48:05.626Z
@21:48:05.65 -> showStatus
@21:48:07.67 -> Cmd.loadCartridge -6
@21:48:07.81 Gate.power :main,:ON,limit to 5000mA
@21:48:07.82 Gate.power :analytic1,:ON,limit to 5000mA
@21:48:07.85 Gate.power :analytic2,:ON,limit to 5000mA
@21:48:07.87 Gate.power :analytic3,:ON,limit to 5000mA
@21:48:08.00 Powering core bus
@21:48:10.28 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@21:48:10.41 <LRAUV> Archive.configure :default
@21:48:10.53 Air.configure :default
@21:48:10.64 Lysis1.configure :default
@21:48:10.74 Lysis2.configure :default
@21:48:10.86 Plunge.configure :default
@21:48:10.98 SamplePump.configure :default
@21:48:11.13 ExtraPump.configure :default
@21:48:11.58 Toroid.configure :default
@21:48:11.70 Carousel.configure :default
@21:48:11.83 Pressure.configure :default
@21:48:11.93 PinPort.configure :default
@21:48:11.99 All elf controllers running firmware version 4.741
@21:48:12.01 Gate.power :motor,:ON,limit to 5000mA
@21:48:12.12 <Archive_home> Archive.in :default
@21:48:12.43 Archive.home.jog 0
@21:48:12.68 <Toroid_home> Toroid.in :default
@21:48:12.71 <Plunge_home> Plunge.in :default
@21:48:12.74 <Lysis1_home> Lysis1.in :default
@21:48:12.79 <PinPort_home> PinPort.in :default
@21:48:12.85 <Lysis2_home> Lysis2.in :default
@21:48:12.88 <Air_home> Air.in :default
@21:48:13.04 <Toroid_home> Toroid.home.jog 0
@21:48:13.10 <PinPort_home> PinPort.home.jog 0
@21:48:13.15 <Plunge_home> Plunge.home.jog 0
@21:48:13.19 <Lysis1_home> Lysis1.home.jog 0
@21:48:13.22 <Air_home> Air.home.jog 0
@21:48:13.25 <Lysis2_home> Lysis2.home.jog 0
@21:48:13.56 <Archive_home> Archive.in :homing
@21:48:13.73 <Toroid_home> Toroid.in :homing
@21:48:13.76 <Plunge_home> Plunge.in :homing
@21:48:13.82 <Archive_home> Archive.jog 4037
@21:48:13.86 <PinPort_home> PinPort.in :homing
@21:48:14.03 <Plunge_home> Plunge.jog 1581
@21:48:14.08 <Toroid_home> Toroid.jog 615
@21:48:14.11 <PinPort_home> PinPort.jog -106
@21:48:14.30 <Air_home> Air.in :homing
@21:48:14.32 <Lysis2_home> Lysis2.in :homing
@21:48:14.35 <Lysis1_home> Lysis1.in :homing
@21:48:14.47 <Lysis2_home> Lysis2.jog 155
@21:48:14.51 <Lysis1_home> Lysis1.jog 1416
@21:48:14.56 <Air_home> Air.jog 167
@21:48:14.67 <PinPort_home> PinPort.setRawPosition! 0
@21:48:14.72 PinPort.seek :home
@21:48:14.80 PinPort.in :default
@21:48:14.96 <Lysis2_home> Lysis2.setRawPosition! 50
@21:48:15.00 Lysis2.seek :home
@21:48:15.09 <Air_home> Air.setRawPosition! 50
@21:48:15.13 Air.seek :home
@21:48:15.28 <Lysis2_home> Lysis2.in :default
@21:48:15.36 <Plunge_home> Plunge.setRawPosition! 50
@21:48:15.42 Plunge.seek :home
@21:48:15.50 <Air_home> Air.in :default
@21:48:15.70 <Plunge_home> Plunge.in :default
@21:48:15.78 <Lysis1_home> Lysis1.setRawPosition! 50
@21:48:15.83 Lysis1.seek :home
@21:48:16.11 Lysis1.in :default
@21:48:16.23 <Archive_home> Archive.setRawPosition! 50
@21:48:16.28 Archive.seek :home
@21:48:16.36 <Toroid_home> Toroid.setRawPosition! 0
@21:48:16.40 Toroid.seek :home
@21:48:16.49 Toroid.in :default
@21:48:16.58 <Archive_home> Archive.in :default
@21:48:16.96 <LRAUV> Toroid.seek :clear
@21:48:18.58 Carousel.in :default
@21:48:18.64 Carousel.home.jog 0
@21:48:19.13 Carousel.in :default
@21:48:19.20 Carousel.seek 21
@21:48:19.35 Selecting Cartridge 24
@21:48:19.59 Carousel.seek 24
@21:48:20.48 Lysis1.in :fast
@21:48:20.54 Lysis1.seek :locked
@21:48:23.14 <Plunge> Plunge.seek :locked
@21:48:23.26 <Air> Air.in :fast
@21:48:23.33 Air.seek :locked
@21:48:25.93 <LRAUV> Powering cartridge bus
@21:48:28.05 Gate.setIR 1750,5000,0,6
@21:48:28.18 Gate.setIR 0,5000,0,6
@21:48:28.23 Gate.power :cartridge,:OFF
@21:48:28.76 Mounted archive Cartridge
@21:48:47.26 -> Cmd.startFiltering
@21:48:47.70 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@21:48:47.72 SamplePump.setPosition! 0ml
@21:48:47.77 Bypass.open
@21:48:49.27 Intake.open
@21:48:50.07 Sea 3.9m deep (20.5psia)
@21:48:50.09 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@21:48:52.67 Vacuum tare is 51% of full scale
@21:48:52.79 Pressure.in :default
@21:48:52.85 Pressure.hold
@21:48:53.07 SamplePump.in :prime
@21:48:53.14 SamplePump.seek 10ml,1:21
@21:49:02.53 Pressure.coast
@21:49:02.58 SamplePump.setPosition! 0ml
@21:49:02.63 Exhaust.open
@21:49:04.05 <closeBypass> Bypass.close
@21:49:05.61 <FILTERING> Pressure.in :default
@21:49:05.67 Pressure.hold
@21:49:05.77 Sea 3.9m deep (20.4psia)
@21:49:05.90 SamplePump.in :prime
@21:49:05.97 SamplePump.seek 100ml,8:51
@21:51:16.25 Pressure.coast
@21:51:16.31 Bypass.open
@21:51:17.02 Intake.close
@21:51:17.87 Toroid.in :hold
@21:51:17.93 Toroid.seek :crack
@21:51:19.48 Toroid.seek :cartridge
@21:51:20.57 Toroid.in :default
@21:51:20.63 Toroid.seek :clear
@21:51:22.58 Pressurizing cartridge to 26psi above 21.8psia
@21:51:22.60 SamplePump.setPosition! 0ml
@21:51:22.64 Intake.open
@21:51:23.38 Bypass.close
@21:51:24.13 Exhaust.close
@21:51:24.91 Pressure.in :default
@21:51:24.97 Pressure.hold
@21:51:25.14 SamplePump.in :charge
@21:51:25.20 SamplePump.seek 2.3ml,42 seconds
@21:51:31.28 Pressure.coast
@21:51:31.33 Intake.close
@21:51:32.14 Pressurized cartridge with 0.163ml to 26.5psi
@21:51:32.15 Isolated @42.7psia (20.9psi)
@21:51:32.17 Verifying cartridge holds at least 0.75..0.85 of 42.7psia
@21:51:32.20 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@21:51:36.82 Sampling 1000.0ml
@21:51:37.25 SamplePump.setPosition! 0ml
@21:51:37.30 Exhaust.open
@21:51:38.01 Intake.open
@21:51:38.85 Pressure.in :default
@21:51:38.91 Pressure.hold
@21:51:39.03 Sea 4.1m deep (20.8psia)
@21:51:39.16 SamplePump.in :sample
@21:51:39.23 SamplePump.seek 1000ml,1:23:53
@21:51:41.89 <sampling> Cartridge 24 filtering 1/1000ml at 0.32ml/s with 29.1psi vacuum
@21:52:41.90 Cartridge 24 filtering 20/1000ml at 0.28ml/s with 29.3psi vacuum 2.4m deep (18.2psia)
@21:53:41.90 Cartridge 24 filtering 38/1000ml at 0.26ml/s with 23.7psi vacuum 3.4m deep (19.7psia)
@21:54:41.90 Cartridge 24 filtering 57/1000ml at 0.31ml/s with 25.7psi vacuum 3.7m deep (20.1psia)
@21:55:41.90 Cartridge 24 filtering 75/1000ml at 0.34ml/s with 22.0psi vacuum 5.1m deep (22.3psia)
@21:56:41.90 Cartridge 24 filtering 93/1000ml at 0.34ml/s with 22.4psi vacuum 5.2m deep (22.4psia)
@21:57:41.90 Cartridge 24 filtering 111/1000ml at 0.27ml/s with 17.5psi vacuum 2.9m deep (19.0psia)
@21:58:41.90 Cartridge 24 filtering 128/1000ml at 0.32ml/s with 29.5psi vacuum 3.4m deep (19.8psia)
@21:59:41.90 Cartridge 24 filtering 145/1000ml at 0.32ml/s with 28.9psi vacuum 2.0m deep (17.7psia)
@22:00:41.90 Cartridge 24 filtering 163/1000ml at 0.30ml/s with 22.5psi vacuum 2.5m deep (18.3psia)
@22:01:41.90 Cartridge 24 filtering 179/1000ml at 0.25ml/s with 29.2psi vacuum 3.2m deep (19.5psia)
@22:02:41.90 Cartridge 24 filtering 196/1000ml at 0.23ml/s with 20.7psi vacuum 3.8m deep (20.3psia)
@22:03:41.90 Cartridge 24 filtering 213/1000ml at 0.28ml/s with 26.4psi vacuum 2.6m deep (18.5psia)
@22:04:41.90 Cartridge 24 filtering 229/1000ml at 0.28ml/s with 28.3psi vacuum 2.6m deep (18.5psia)
@22:05:41.90 Cartridge 24 filtering 246/1000ml at 0.29ml/s with 23.4psi vacuum 4.1m deep (20.7psia)
@22:06:41.90 Cartridge 24 filtering 262/1000ml at 0.27ml/s with 24.5psi vacuum
@22:07:41.90 Cartridge 24 filtering 278/1000ml at 0.25ml/s with 27.4psi vacuum 5.4m deep (22.6psia)
@22:08:41.90 Cartridge 24 filtering 293/1000ml at 0.27ml/s with 28.3psi vacuum
@22:09:41.90 Cartridge 24 filtering 309/1000ml at 0.28ml/s with 23.7psi vacuum 3.2m deep (19.4psia)
@22:10:41.90 Cartridge 24 filtering 324/1000ml at 0.22ml/s with 28.1psi vacuum 4.2m deep (20.9psia)
@22:11:41.90 Cartridge 24 filtering 340/1000ml at 0.23ml/s with 21.5psi vacuum
@22:12:41.90 Cartridge 24 filtering 355/1000ml at 0.25ml/s with 28.5psi vacuum 2.4m deep (18.3psia)
@22:13:41.90 Cartridge 24 filtering 370/1000ml at 0.28ml/s with 24.8psi vacuum 6.0m deep (23.6psia)
@22:14:41.90 Cartridge 24 filtering 384/1000ml at 0.24ml/s with 18.6psi vacuum 2.9m deep (19.1psia)
@22:15:41.90 Cartridge 24 filtering 399/1000ml at 0.22ml/s with 23.0psi vacuum 4.2m deep (20.8psia)
@22:16:41.90 Cartridge 24 filtering 413/1000ml at 0.25ml/s with 25.9psi vacuum 3.0m deep (19.2psia)
@22:17:41.90 Cartridge 24 filtering 428/1000ml at 0.24ml/s with 25.4psi vacuum
@22:18:41.90 Cartridge 24 filtering 442/1000ml at 0.22ml/s with 24.1psi vacuum 4.9m deep (21.9psia)
@22:19:41.90 Cartridge 24 filtering 456/1000ml at 0.27ml/s with 23.3psi vacuum 5.4m deep (22.7psia)
@22:20:41.90 Cartridge 24 filtering 469/1000ml at 0.23ml/s with 19.6psi vacuum 4.5m deep (21.3psia)
@22:21:41.90 Cartridge 24 filtering 483/1000ml at 0.21ml/s with 24.1psi vacuum 4.5m deep (21.4psia)
@22:22:41.90 Cartridge 24 filtering 497/1000ml at 0.23ml/s with 24.0psi vacuum 4.1m deep (20.8psia)
@22:23:41.90 Cartridge 24 filtering 510/1000ml at 0.25ml/s with 25.6psi vacuum 1.7m deep (17.3psia)
@22:24:41.90 Cartridge 24 filtering 523/1000ml at 0.22ml/s with 26.3psi vacuum 3.6m deep (20.1psia)
@22:25:41.90 Cartridge 24 filtering 536/1000ml at 0.22ml/s with 24.4psi vacuum 1.9m deep (17.6psia)
@22:26:41.90 Cartridge 24 filtering 549/1000ml at 0.22ml/s with 26.4psi vacuum 2.1m deep (17.8psia)
@22:27:41.90 Cartridge 24 filtering 562/1000ml at 0.25ml/s with 20.1psi vacuum 8.4m deep (27.1psia)
@22:28:41.90 Cartridge 24 filtering 575/1000ml at 0.25ml/s with 23.5psi vacuum 3.9m deep (20.4psia)
@22:29:41.90 Cartridge 24 filtering 588/1000ml at 0.20ml/s with 24.2psi vacuum 2.4m deep (18.2psia)
@22:30:41.90 Cartridge 24 filtering 600/1000ml at 0.23ml/s with 26.3psi vacuum 4.9m deep (21.9psia)
@22:31:41.90 Cartridge 24 filtering 613/1000ml at 0.21ml/s with 23.8psi vacuum 3.8m deep (20.3psia)
@22:32:41.90 Cartridge 24 filtering 625/1000ml at 0.20ml/s with 26.7psi vacuum 4.2m deep (20.9psia)
@22:33:16.21 <FILTERING> Flow averaged 0.25ml/s over 41:38
@22:33:16.24 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi
@22:33:16.81 Relieved excess vacuum in .6 seconds
@22:33:16.84 SamplePump.seek 1000ml,31:10
@22:33:27.48 Flow averaged 0.20ml/s over 11 seconds
@22:33:27.50 <closeIntake> Intake.close
@22:33:27.53 <closeExhaust> Exhaust.close
@22:33:27.74 <FILTERING> Toroid.in :hold
@22:33:27.80 Toroid.seek :bypass
@22:33:29.43 Toroid.in :default
@22:33:29.49 Toroid.seek :clear
@22:33:31.10 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@22:33:31.11 Pressure.coast
@22:33:31.29 Sampled  634.5ml
@22:33:33.58 <LRAUV> -> Cmd.startProcessing
@22:33:33.94 <PROCESSING> Plunge.seek :reagent
@22:33:34.78 Sample loop closed. Ready to Deliver Reagents
@22:33:34.79 Archive.seek :sealed
@22:33:39.06 Archive.seek :evac
@22:33:53.00 delay 1:00
@22:34:53.02 Archive.seek :clear
@22:35:10.74 RNAlater soak for 10:00
@22:45:10.77 Archive.seek :tVent
@22:45:13.35 delay 1 second
@22:45:14.37 Archive.seek :evac
@22:45:29.99 delay 10 seconds
@22:45:40.02 Sample preserved and evacuated
@22:45:41.22 <LRAUV> -> Cmd.stop
@22:45:41.36 Carousel.seek 24
@22:45:41.53 Plunge.seek :locked
@22:45:42.47 <Archive> Archive.in :unlocking
@22:45:42.59 Archive.seek :clear
@22:45:42.67 <Air> Air.in :unlocking
@22:45:42.78 Air.seek :clear
@22:45:42.85 <Lysis1> Lysis1.in :unlocking
@22:45:42.97 Lysis1.seek :clear
@22:45:46.64 <LRAUV> Archive.coast
@22:45:46.68 Air.coast
@22:45:46.73 Lysis1.coast
@22:45:46.78 Lysis2.coast
@22:45:46.83 Plunge.coast
@22:45:46.88 SamplePump.coast
@22:45:46.93 Toroid.coast
@22:45:46.98 Carousel.coast
@22:45:47.03 PinPort.coast
@22:45:47.21 Gate.power :motor,:OFF
@22:45:47.23 Gate.power :core,:OFF
@22:45:47.25 Gate.power :analytic1,:OFF
@22:45:47.27 Gate.power :analytic2,:OFF
@22:45:47.29 Gate.power :analytic3,:OFF
@22:45:47.31 Gate.power :main,:OFF
@22:45:47.33 Safely stopped and ready to power off
##-- file closed 2021-04-24T05:58:14.894Z

##-- file opened 2021-04-24T05:58:14.895Z
@22:58:15.53 -> showStatus
@22:58:17.55 -> Cmd.loadCartridge -9
@22:58:17.69 Gate.power :main,:ON,limit to 5000mA
@22:58:17.71 Gate.power :analytic1,:ON,limit to 5000mA
@22:58:17.73 Gate.power :analytic2,:ON,limit to 5000mA
@22:58:17.75 Gate.power :analytic3,:ON,limit to 5000mA
@22:58:17.88 Powering core bus
@22:58:20.16 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@22:58:20.29 <LRAUV> Archive.configure :default
@22:58:20.41 Air.configure :default
@22:58:20.51 Lysis1.configure :default
@22:58:20.62 Lysis2.configure :default
@22:58:20.74 Plunge.configure :default
@22:58:20.86 SamplePump.configure :default
@22:58:21.36 ExtraPump.configure :default
@22:58:21.47 Toroid.configure :default
@22:58:21.59 Carousel.configure :default
@22:58:21.71 Pressure.configure :default
@22:58:21.81 PinPort.configure :default
@22:58:21.87 All elf controllers running firmware version 4.741
@22:58:21.90 Gate.power :motor,:ON,limit to 5000mA
@22:58:22.05 <Archive_home> Archive.in :default
@22:58:22.35 Archive.home.jog 0
@22:58:22.53 <Toroid_home> Toroid.in :default
@22:58:22.61 <Plunge_home> Plunge.in :default
@22:58:22.65 <Lysis1_home> Lysis1.in :default
@22:58:22.70 <Air_home> Air.in :default
@22:58:22.75 <Lysis2_home> Lysis2.in :default
@22:58:22.81 <PinPort_home> PinPort.in :default
@22:58:22.96 <Toroid_home> Toroid.home.jog 0
@22:58:23.00 <Plunge_home> Plunge.home.jog 0
@22:58:23.03 <Lysis1_home> Lysis1.home.jog 0
@22:58:23.06 <Air_home> Air.home.jog 0
@22:58:23.08 <Lysis2_home> Lysis2.home.jog 0
@22:58:23.15 <PinPort_home> PinPort.home.jog 0
@22:58:23.52 <Archive_home> Archive.in :homing
@22:58:23.79 <Toroid_home> Toroid.in :homing
@22:58:23.83 <Plunge_home> Plunge.in :homing
@22:58:23.86 <Air_home> Air.in :homing
@22:58:23.90 <PinPort_home> PinPort.in :homing
@22:58:23.93 <Lysis1_home> Lysis1.in :homing
@22:58:23.97 <Archive_home> Archive.jog 155
@22:58:24.01 <Lysis2_home> Lysis2.in :homing
@22:58:24.20 <Toroid_home> Toroid.jog 612
@22:58:24.22 <Plunge_home> Plunge.jog 1581
@22:58:24.32 <PinPort_home> PinPort.jog -107
@22:58:24.36 <Lysis2_home> Lysis2.jog 1428
@22:58:24.38 <Lysis1_home> Lysis1.jog 1417
@22:58:24.40 <Air_home> Air.jog 157
@22:58:24.62 <Archive_home> Archive.setRawPosition! 50
@22:58:24.66 Archive.seek :home
@22:58:24.74 <PinPort_home> PinPort.setRawPosition! 0
@22:58:24.79 PinPort.seek :home
@22:58:24.88 PinPort.in :default
@22:58:24.97 <Archive_home> Archive.in :default
@22:58:25.05 <Air_home> Air.setRawPosition! 50
@22:58:25.09 Air.seek :home
@22:58:25.38 Air.in :default
@22:58:25.69 <Plunge_home> Plunge.setRawPosition! 50
@22:58:25.71 <Lysis2_home> Lysis2.setRawPosition! 50
@22:58:25.79 <Plunge_home> Plunge.seek :home
@22:58:25.83 <Lysis2_home> Lysis2.seek :home
@22:58:25.97 <Lysis1_home> Lysis1.setRawPosition! 50
@22:58:26.01 Lysis1.seek :home
@22:58:26.11 <Plunge_home> Plunge.in :default
@22:58:26.20 <Lysis2_home> Lysis2.in :default
@22:58:26.29 <Lysis1_home> Lysis1.in :default
@22:58:26.37 <Toroid_home> Toroid.setRawPosition! 0
@22:58:26.42 Toroid.seek :home
@22:58:26.51 Toroid.in :default
@22:58:26.69 <LRAUV> Toroid.seek :clear
@22:58:28.31 Carousel.in :default
@22:58:28.37 Carousel.home.jog 0
@22:58:28.87 Carousel.in :default
@22:58:28.93 Carousel.seek 24
@22:58:29.10 Selecting Cartridge 19
@22:58:29.34 Carousel.seek 19
@22:58:30.63 Lysis1.in :fast
@22:58:30.70 Lysis1.seek :locked
@22:58:33.29 <Plunge> Plunge.seek :locked
@22:58:33.43 <Air> Air.in :fast
@22:58:33.49 Air.seek :locked
@22:58:36.10 <LRAUV> Powering cartridge bus
@22:58:38.22 Gate.setIR 1750,5000,0,6
@22:58:38.35 Gate.setIR 0,5000,0,6
@22:58:38.41 PuckHeater.configure :default
@22:58:38.46 Gate.power :heat,:ON,limit to 5000mA
@22:58:39.12 Mounted lysis Cartridge board#Q05036
@22:58:39.15 Using default puck heat loss curve for HeatingAccelInCarousel
@22:58:57.95 -> Cmd.startFiltering
@22:58:58.40 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@22:58:58.42 SamplePump.setPosition! 0ml
@22:58:58.46 Bypass.open
@22:58:59.95 Intake.open
@22:59:00.75 Sea 7.2m deep (25.3psia)
@22:59:00.77 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@22:59:03.35 Vacuum tare is 51% of full scale
@22:59:03.48 Pressure.in :default
@22:59:03.54 Pressure.hold
@22:59:03.77 SamplePump.in :prime
@22:59:03.83 SamplePump.seek 10ml,1:21
@22:59:13.22 Pressure.coast
@22:59:13.27 SamplePump.setPosition! 0ml
@22:59:13.32 Exhaust.open
@22:59:14.71 <closeBypass> Bypass.close
@22:59:16.24 <FILTERING> Pressure.in :default
@22:59:16.30 Pressure.hold
@22:59:16.41 Sea 6.5m deep (24.3psia)
@22:59:16.54 SamplePump.in :prime
@22:59:16.60 SamplePump.seek 100ml,8:51
@23:01:26.86 Pressure.coast
@23:01:26.92 Bypass.open
@23:01:27.64 Intake.close
@23:01:28.50 Toroid.in :hold
@23:01:28.56 Toroid.seek :crack
@23:01:30.13 Toroid.seek :cartridge
@23:01:31.22 Toroid.in :default
@23:01:31.28 Toroid.seek :clear
@23:01:33.25 Pressurizing cartridge to 26psi above 25.3psia
@23:01:33.26 SamplePump.setPosition! 0ml
@23:01:33.30 Intake.open
@23:01:34.02 Bypass.close
@23:01:34.75 Exhaust.close
@23:01:35.54 Pressure.in :default
@23:01:35.60 Pressure.hold
@23:01:35.77 SamplePump.in :charge
@23:01:35.83 SamplePump.seek 2.3ml,42 seconds
@23:01:44.27 Pressure.coast
@23:01:44.32 Intake.close
@23:01:45.11 Pressurized cartridge with 0.315ml to 26.6psi
@23:01:45.12 Isolated @46.4psia (21.1psi)
@23:01:45.13 Verifying cartridge holds at least 0.75..0.85 of 46.4psia
@23:01:45.17 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@23:01:49.79 Sampling 1000.0ml
@23:01:49.81 SamplePump.setPosition! 0ml
@23:01:49.86 Exhaust.open
@23:01:50.60 Intake.open
@23:01:51.42 Pressure.in :default
@23:01:51.49 Pressure.hold
@23:01:51.61 Sea 7.4m deep (25.6psia)
@23:01:51.74 SamplePump.in :sample
@23:01:51.81 SamplePump.seek 1000ml,1:23:53
@23:01:54.46 <sampling> Cartridge 19 filtering 1/1000ml at 0.33ml/s with 20.2psi vacuum 5.3m deep (22.5psia)
@23:02:54.46 Cartridge 19 filtering 19/1000ml at 0.29ml/s with 26.5psi vacuum
@23:03:54.47 Cartridge 19 filtering 37/1000ml at 0.32ml/s with 24.7psi vacuum 4.0m deep (20.7psia)
@23:04:54.46 Cartridge 19 filtering 54/1000ml at 0.26ml/s with 27.3psi vacuum 2.5m deep (18.4psia)
@23:05:54.47 Cartridge 19 filtering 71/1000ml at 0.29ml/s with 27.8psi vacuum
@23:06:54.46 Cartridge 19 filtering 88/1000ml at 0.30ml/s with 20.4psi vacuum 5.9m deep (23.5psia)
@23:07:54.47 Cartridge 19 filtering 104/1000ml at 0.27ml/s with 20.5psi vacuum 3.7m deep (20.2psia)
@23:08:54.46 Cartridge 19 filtering 120/1000ml at 0.28ml/s with 25.9psi vacuum 2.5m deep (18.4psia)
@23:09:54.47 Cartridge 19 filtering 136/1000ml at 0.28ml/s with 23.7psi vacuum 5.1m deep (22.2psia)
@23:10:54.46 Cartridge 19 filtering 151/1000ml at 0.26ml/s with 22.4psi vacuum 2.8m deep (18.8psia)
@23:11:54.47 Cartridge 19 filtering 167/1000ml at 0.23ml/s with 20.6psi vacuum 3.8m deep (20.4psia)
@23:12:54.46 Cartridge 19 filtering 182/1000ml at 0.26ml/s with 22.5psi vacuum 5.7m deep (23.1psia)
@23:13:54.47 Cartridge 19 filtering 196/1000ml at 0.24ml/s with 27.6psi vacuum
@23:14:54.46 Cartridge 19 filtering 211/1000ml at 0.25ml/s with 23.1psi vacuum 3.9m deep (20.5psia)
@23:15:54.47 Cartridge 19 filtering 225/1000ml at 0.27ml/s with 23.5psi vacuum 5.2m deep (22.4psia)
@23:16:54.46 Cartridge 19 filtering 239/1000ml at 0.20ml/s with 23.9psi vacuum 1.7m deep (17.2psia)
@23:17:54.47 Cartridge 19 filtering 253/1000ml at 0.26ml/s with 24.8psi vacuum 4.9m deep (21.9psia)
@23:18:54.46 Cartridge 19 filtering 267/1000ml at 0.20ml/s with 25.9psi vacuum 3.3m deep (19.6psia)
@23:19:54.47 Cartridge 19 filtering 280/1000ml at 0.23ml/s with 23.7psi vacuum
@23:20:54.46 Cartridge 19 filtering 294/1000ml at 0.23ml/s with 23.3psi vacuum 5.2m deep (22.3psia)
@23:21:54.47 Cartridge 19 filtering 307/1000ml at 0.21ml/s with 22.6psi vacuum 3.5m deep (19.9psia)
@23:22:54.46 Cartridge 19 filtering 320/1000ml at 0.21ml/s with 22.2psi vacuum 2.7m deep (18.7psia)
@23:23:54.47 Cartridge 19 filtering 333/1000ml at 0.20ml/s with 25.2psi vacuum 4.9m deep (21.9psia)
@23:24:54.46 Cartridge 19 filtering 346/1000ml at 0.21ml/s with 23.6psi vacuum 2.2m deep (18.0psia)
@23:25:54.47 Cartridge 19 filtering 359/1000ml at 0.21ml/s with 23.1psi vacuum 3.8m deep (20.4psia)
@23:26:54.46 Cartridge 19 filtering 371/1000ml at 0.20ml/s with 26.6psi vacuum 4.6m deep (21.4psia)
@23:27:54.47 Cartridge 19 filtering 384/1000ml at 0.20ml/s with 29.0psi vacuum
@23:28:54.46 Cartridge 19 filtering 396/1000ml at 0.22ml/s with 24.1psi vacuum 4.6m deep (21.4psia)
@23:29:54.47 Cartridge 19 filtering 409/1000ml at 0.20ml/s with 24.9psi vacuum 4.0m deep (20.6psia)
@23:30:54.46 Cartridge 19 filtering 421/1000ml at 0.20ml/s with 25.7psi vacuum 1.6m deep (17.1psia)
@23:31:54.47 Cartridge 19 filtering 433/1000ml at 0.20ml/s with 25.4psi vacuum 4.7m deep (21.6psia)
@23:32:39.10 <FILTERING> Flow averaged 0.24ml/s over 30:48
@23:32:39.13 Waiting up to 8 seconds for vacuum to reduce from 26.0 to 18.0psi
@23:32:39.69 Relieved excess vacuum in .6 seconds
@23:32:39.72 SamplePump.seek 1000ml,47:02
@23:32:54.46 <sampling> Cartridge 19 filtering 445/1000ml at 0.20ml/s with 27.6psi vacuum 3.9m deep (20.5psia)
@23:33:14.44 <FILTERING> Flow averaged 0.20ml/s over 35 seconds
@23:33:14.47 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi
@23:33:15.29 Relieved excess vacuum in .9 seconds
@23:33:15.32 SamplePump.seek 1000ml,46:27
@23:33:18.46 Flow estimate reduced to 0.16ml/s
@23:33:18.49 <closeIntake> Intake.close
@23:33:18.55 <closeExhaust> Exhaust.close
@23:33:18.73 <FILTERING> Toroid.in :hold
@23:33:18.79 Toroid.seek :bypass
@23:33:20.42 Toroid.in :default
@23:33:20.48 Toroid.seek :clear
@23:33:22.09 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@23:33:22.10 Pressure.coast
@23:33:22.29 Sampled  449.6ml
@23:33:24.23 <LRAUV> -> Cmd.startProcessing
@23:33:25.66 <PROCESSING> SPR.command \"read_sequences\"
@23:33:26.43 SPR.command \"stats_on\"
@23:33:26.47 SPR.command \"start 0 1\"
@23:33:28.48 <SPRlogger> Begun log /var/log/esp/spr21apr2323h.spr
@23:33:29.50 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3)
@23:33:31.17 <PROCESSING> Handoff.select :waste
@23:33:37.87 Syringe tare is 20% of full scale
@23:33:37.89 PinPort.in :locking
@23:33:37.96 PinPort.seek :locked
@23:33:38.43 <posArchive> Archive.in :fast
@23:33:38.49 <posLyS2> Lysis2.in :fast
@23:33:38.54 <PROCESSING> Lysis1.seek 1350uL
@23:33:38.65 <posArchive> Archive.seek :full
@23:33:38.70 <posLyS2> Lysis2.seek :full
@23:33:40.78 <PROCESSING> Plunge.seek :reagent
@23:33:41.68 Evacuating puck with filter bubble point of 50psi
@23:33:41.70 PinPort.seek :locked
@23:33:41.78 Handoff.select :waste
@23:33:48.36 Air syringe pumping up to 1 stroke to reach 8psi
@23:33:48.37 Air stroke #1 of 1
@23:33:48.45 Air.in :pressurize
@23:33:48.51 Air.seek :pressurized
@23:33:59.77 0.3psi after pressurize
@23:33:59.78 Air.in :compress
@23:33:59.85 Air.seek :evac
@23:34:03.93 0.5psi after compress
@23:34:16.50 0.2psi after compress (stable)
@23:34:16.52 Air.in :default
@23:34:16.59 Air.hold
@23:34:16.76 at 0.2psi Opened to waste
@23:34:16.77 Handoff.select :blocked
@23:34:16.90 Archive syringe pumping up to 5 strokes to reach 50psi
@23:34:16.91 Archive stroke #1 of 5
@23:34:16.99 Archive.in :pressurize
@23:34:17.07 Archive.seek :pressurized
@23:34:29.82 11.0psi after pressurize
@23:34:29.84 Archive.in :compress
@23:34:29.91 Archive.seek :empty
@23:34:45.80 32.9psi after compress
@23:35:05.89 41.2psi after compress (stable)
@23:35:05.91 Archive.in :gulp
@23:35:05.98 Archive.seek :topVent
@23:35:13.19 41.9psi after gulp
@23:35:25.76 41.2psi after gulp (stable)
@23:35:25.77 Archive stroke #2 of 5
@23:35:25.86 Archive.in :pressurize
@23:35:25.92 Archive.seek :pressurized
@23:35:38.66 40.0psi after pressurize
@23:35:38.67 Archive.in :compress
@23:35:38.75 Archive.seek :empty
@23:35:51.18 Reached 50.1 of 50.0psi after pumping 3387uL (1.96 strokes)
@23:35:51.19 Archive.in :default
@23:35:51.26 Archive.hold
@23:35:51.32 Handoff.select :waste
@23:36:02.51 delay 1:00
@23:37:02.53 Handoff.select :blocked
@23:37:25.24 Handoff.select :waste
@23:37:25.38 1.6psi at evacEnd
@23:37:25.39 Handoff.select :blocked
@23:37:25.52 Archive.in :fast
@23:37:25.59 Archive.seek :topVent
@23:37:39.04 Stable @2.5psi after Handoff blocked
@23:37:39.06 Archive syringe pumping up to 5 strokes to reach 32psi
@23:37:39.08 Archive stroke #1 of 5
@23:37:39.17 Archive.in :pressurize
@23:37:39.23 Archive.seek :pressurized
@23:37:51.97 3.4psi after pressurize
@23:37:51.98 Archive.in :compress
@23:37:52.06 Archive.seek :empty
@23:38:07.94 4.8psi after compress
@23:39:10.51 22.9psi after compress (stable)
@23:39:10.53 Archive.in :gulp
@23:39:10.60 Archive.seek :topVent
@23:39:17.81 24.2psi after gulp
@23:39:30.39 25.2psi after gulp (stable)
@23:39:30.40 Archive stroke #2 of 5
@23:39:30.49 Archive.in :pressurize
@23:39:30.55 Archive.seek :pressurized
@23:39:43.29 26.4psi after pressurize
@23:39:43.30 Archive.in :compress
@23:39:43.39 Archive.seek :empty
@23:39:59.28 28.4psi after compress
@23:40:20.44 Reached 32.5 of 32.5psi after pumping 3460uL (2.00 strokes)
@23:40:20.46 Archive.in :default
@23:40:20.53 Archive.hold
@23:40:20.59 Handoff.select :waste
@23:40:34.78 delay 1:00
@23:41:34.80 Handoff.select :blocked
@23:41:57.47 Handoff.select :waste
@23:41:57.59 2.3psi at evacEnd
@23:41:57.60 Air.seek :fullVent
@23:41:59.07 Archive.in :fast
@23:41:59.13 Archive.seek :topVent
@23:42:06.27 Seawater evacuated, Presure above/below filter released. Lysis begins
@23:42:06.36 Lysis1.in :slow
@23:42:06.42 Lysis1.seek 1050uL
@23:42:36.46 delay 5 seconds
@23:42:41.49 Handoff.select :blocked
@23:42:41.65 Lysis1.seek 450uL
@23:43:41.65 delay 3 seconds
@23:43:44.72 Air.in to mix into 1.37806 parts Lysis1
@23:43:45.14 <Airmixer> Air.seek :evac
@23:43:45.18 <PROCESSING> Lysis1.seek 300uL
@23:44:00.30 Lysis1.seek :empty
@23:44:30.42 Lysis buffer delivered @15.1psi
@23:44:30.43 Polling Can every 2:00
@23:44:30.98 PuckHeater.configure :default #ambient 11.5C
@23:44:31.03 PuckHeater.seek 80.2C
@23:45:41.56 PuckHeater.hold 2:00,80.2C
@23:47:41.46 Polling Can every 2:00
@23:47:42.01 PuckHeater.configure :default #ambient 11.6C
@23:47:42.05 PuckHeater.hold 30 seconds,79.2C
@23:48:12.23 Polling Can every 2:00
@23:48:12.79 PuckHeater.hold 30 seconds,78.7C
@23:48:42.95 Polling Can every 2:00
@23:48:43.50 PuckHeater.configure :default #ambient 11.5C
@23:48:43.55 PuckHeater.hold 10:00,78.1C
@23:58:42.91 Lysis complete. Syringe pressure @17.4psi
@23:58:42.92 Handoff.select :blocked
@23:58:42.96 Archive syringe pumping up to 3 strokes to reach 40psi
@23:58:42.98 Archive stroke #1 of 3
@23:58:43.07 Archive.in :pressurize
@23:58:43.15 Archive.seek :pressurized
@23:58:55.89 17.0psi after pressurize
@23:58:55.90 Archive.in :compress
@23:58:55.98 Archive.seek :empty
@23:59:04.60 Reached 40.4 of 40.0psi after pumping 1575uL (0.91 strokes)
@23:59:04.61 Archive.in :default
@23:59:04.68 Archive.hold
@23:59:04.75 delay 2 seconds
@23:59:06.77 Archive.in :fast
@23:59:06.83 Archive.seek :full
@23:59:13.50 Lysis1.seek 138uL
@23:59:27.38 delay 1 second
@23:59:28.41 <fillArchive> Archive.seek :full
@23:59:28.43 <PROCESSING> Lysis1.seek :empty
@23:59:42.42 Initial air pressure @45.2psi
@23:59:42.49 Lysis1.in :extract
@23:59:42.57 Lysis1.seek 600uL,nil
@23:59:44.28 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@23:59:44.29 Archive stroke #1 of 6
@23:59:44.37 Archive.in :pressurize
@23:59:44.46 Archive.seek :pressurized
@23:59:57.21 15.7psi after pressurize
@23:59:57.22 Archive.in :compress
@23:59:57.30 Archive.seek :empty
@00:00:13.22PDT24-Apr-21 15.2psi after compress
@00:00:14.70 Archive.in :default
@00:00:14.74 Archive.hold
@00:00:14.89 <PROCESSING> Initial 600uL lysate @16.2psi
@00:00:14.90 Handoff.select :blocked
@00:00:15.00 Initial air pressure @17.0psi
@00:00:15.08 Lysis1.seek 825uL,nil
@00:00:16.26 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@00:00:16.27 Archive stroke #1 of 6
@00:00:16.35 Archive.in :compress
@00:00:16.44 Archive.seek :empty
@00:00:16.59 15.2psi after compress
@00:00:17.06 Reached 17.3 of 17.0psi after pumping 1730uL (1.00 strokes)
@00:00:17.07 Archive.in :default
@00:00:17.14 Archive.hold
@00:00:17.94 Archive syringe pumping up to 6 strokes to reach 17psi
@00:00:17.95 Archive stroke #1 of 6
@00:00:18.03 Archive.in :compress
@00:00:18.10 Archive.seek :empty
@00:00:18.29 15.4psi after compress
@00:00:18.82 Reached 17.1 of 17.0psi after pumping 1730uL (1.00 strokes)
@00:00:18.83 Archive.in :default
@00:00:18.89 Archive.hold
@00:00:19.62 Archive syringe pumping up to 6 strokes to reach 17psi
@00:00:19.63 Archive stroke #1 of 6
@00:00:19.76 Archive.in :compress
@00:00:19.82 Archive.seek :empty
@00:00:19.99 15.3psi after compress
@00:00:32.57 15.8psi after compress (stable)
@00:00:37.70 Recovering 3.1uL/s
@00:00:42.09 Archive.in :default
@00:00:42.13 Archive.hold
@00:00:42.39 <PROCESSING> Recovered 825uL lysate @16.0psi
@00:00:42.41 Air.in :halfSpeed
@00:00:42.48 Air.seek :fullVent
@00:00:44.73 Puck Top Vented, bottom @3.2psi
@00:00:44.74 Handoff.select :waste
@00:00:44.86 delay 5 seconds
@00:00:49.92 Handoff open to waste, bottom @0.1psi
@00:00:49.94 Air.in :default
@00:00:50.00 Air.seek :evac
@00:00:51.44 Lysis2.in :DAmix
@00:00:51.54 Lysis1.in :DAmix
@00:00:52.02 <Lysis2mixer> Lysis2.seek 1480uL
@00:00:52.07 <PROCESSING> Lysis1.seek 575uL
@00:01:27.12 delay 5 seconds
@00:01:32.14 Handoff.select SPR
@00:01:32.26 SPR.command \"start 2 14\"
@00:01:33.45 delay 10 seconds
@00:01:43.47 SPR.command \"start 1 0\"
@00:01:46.71 Waiting up to 4:00 for SPR to enter state 1
@00:03:49.54 SPR.command \"hold_on\"
@00:03:50.96 SPR Dilution 1 of 1 -- Running Experiment 1
@00:03:51.39 <Lysis2mixer> Lysis2.seek 1180uL
@00:03:51.46 <PROCESSING> Lysis1.seek 275uL
@00:04:33.47 volL1 = 300.0 and volL2 = 300.0
@00:04:33.48 delay 15 seconds
@00:04:48.50 SPR.command \"hold_off\"
@00:04:52.09 Waiting up to 38:00 for SPR to enter state 7
@00:31:36.09 SPR Analysis has Completed
@00:31:36.11 Handoff.select :waste
@00:31:37.03 <SPRlogger> Ended log /var/log/esp/spr21apr2323h.spr
@00:31:37.04 <PROCESSING> SPR.command \"stats_off\"
@00:31:38.59 <LRAUV> -> Cmd.stop
@00:31:38.68 <closeHandoff> Handoff.select :close
@00:31:38.80 <LRAUV> Gate.power :heat,:OFF
@00:31:38.82 Gate.power :cartridge,:OFF
@00:31:38.89 Carousel.seek 19
@00:31:39.07 Plunge.seek :locked
@00:31:39.95 PinPort.in :unlocking
@00:31:40.01 PinPort.seek :clear
@00:31:40.55 <Archive> Archive.in :unlocking
@00:31:40.68 Archive.seek :clear
@00:31:40.73 <Air> Air.in :unlocking
@00:31:40.88 Air.seek :clear
@00:31:40.96 <Lysis1> Lysis1.in :unlocking
@00:31:41.07 Lysis1.seek :clear
@00:31:41.13 <Lysis2> Lysis2.in :unlocking
@00:31:41.21 Lysis2.seek :clear
@00:31:44.73 <LRAUV> Archive.coast
@00:31:44.78 Air.coast
@00:31:44.83 Lysis1.coast
@00:31:44.88 Lysis2.coast
@00:31:44.93 Plunge.coast
@00:31:44.98 SamplePump.coast
@00:31:45.03 Toroid.coast
@00:31:45.08 Carousel.coast
@00:31:45.14 PinPort.coast
@00:31:45.32 Gate.power :motor,:OFF
@00:31:45.34 Gate.power :core,:OFF
@00:31:45.36 Gate.power :analytic1,:OFF
@00:31:45.38 Gate.power :analytic2,:OFF
@00:31:45.40 Gate.power :analytic3,:OFF
@00:31:45.42 Gate.power :main,:OFF
@00:31:45.44 Safely stopped and ready to power off
##-- file closed 2021-04-24T09:38:42.366Z

##-- file opened 2021-04-24T09:38:42.371Z
@02:38:42.69 -> showStatus
@02:38:44.71 -> Cmd.loadCartridge -6
@02:38:44.85 Gate.power :main,:ON,limit to 5000mA
@02:38:44.86 Gate.power :analytic1,:ON,limit to 5000mA
@02:38:44.88 Gate.power :analytic2,:ON,limit to 5000mA
@02:38:44.91 Gate.power :analytic3,:ON,limit to 5000mA
@02:38:45.04 Powering core bus
@02:38:47.32 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@02:38:47.45 <LRAUV> Archive.configure :default
@02:38:47.57 Air.configure :default
@02:38:47.68 Lysis1.configure :default
@02:38:47.78 Lysis2.configure :default
@02:38:47.90 Plunge.configure :default
@02:38:48.37 SamplePump.configure :default
@02:38:48.52 ExtraPump.configure :default
@02:38:48.63 Toroid.configure :default
@02:38:48.75 Carousel.configure :default
@02:38:48.88 Pressure.configure :default
@02:38:48.98 PinPort.configure :default
@02:38:49.04 All elf controllers running firmware version 4.741
@02:38:49.06 Gate.power :motor,:ON,limit to 5000mA
@02:38:49.20 <Archive_home> Archive.in :default
@02:38:49.53 Archive.home.jog 0
@02:38:49.69 <Toroid_home> Toroid.in :default
@02:38:49.76 <Plunge_home> Plunge.in :default
@02:38:49.80 <Lysis1_home> Lysis1.in :default
@02:38:49.83 <Air_home> Air.in :default
@02:38:49.87 <PinPort_home> PinPort.in :default
@02:38:49.96 <Lysis2_home> Lysis2.in :default
@02:38:50.10 <Toroid_home> Toroid.home.jog 0
@02:38:50.14 <Lysis1_home> Lysis1.home.jog 0
@02:38:50.18 <Plunge_home> Plunge.home.jog 0
@02:38:50.21 <PinPort_home> PinPort.home.jog 0
@02:38:50.23 <Air_home> Air.home.jog 0
@02:38:50.27 <Lysis2_home> Lysis2.home.jog 0
@02:38:50.81 <Archive_home> Archive.in :homing
@02:38:51.01 <Toroid_home> Toroid.in :homing
@02:38:51.04 <Plunge_home> Plunge.in :homing
@02:38:51.07 <Air_home> Air.in :homing
@02:38:51.12 <Lysis1_home> Lysis1.in :homing
@02:38:51.15 <Archive_home> Archive.jog 188
@02:38:51.18 <PinPort_home> PinPort.in :homing
@02:38:51.21 <Lysis2_home> Lysis2.in :homing
@02:38:51.42 <Plunge_home> Plunge.jog 1578
@02:38:51.46 <Toroid_home> Toroid.jog 615
@02:38:51.49 <Air_home> Air.jog 173
@02:38:51.53 <Lysis1_home> Lysis1.jog 1417
@02:38:51.57 <PinPort_home> PinPort.jog -107
@02:38:51.60 <Lysis2_home> Lysis2.jog 152
@02:38:51.84 <Archive_home> Archive.setRawPosition! 50
@02:38:51.88 Archive.seek :home
@02:38:51.97 <PinPort_home> PinPort.setRawPosition! 0
@02:38:52.01 PinPort.seek :home
@02:38:52.11 <Air_home> Air.setRawPosition! 50
@02:38:52.13 <PinPort_home> PinPort.in :default
@02:38:52.22 <Air_home> Air.seek :home
@02:38:52.32 <Lysis2_home> Lysis2.setRawPosition! 50
@02:38:52.34 <Archive_home> Archive.in :default
@02:38:52.41 <Lysis2_home> Lysis2.seek :home
@02:38:52.54 <Air_home> Air.in :default
@02:38:52.73 <Plunge_home> Plunge.setRawPosition! 50
@02:38:52.75 <Lysis2_home> Lysis2.in :default
@02:38:52.82 <Plunge_home> Plunge.seek :home
@02:38:52.95 <Lysis1_home> Lysis1.setRawPosition! 50
@02:38:53.00 Lysis1.seek :home
@02:38:53.14 <Plunge_home> Plunge.in :default
@02:38:53.27 <Lysis1_home> Lysis1.in :default
@02:38:53.74 <Toroid_home> Toroid.setRawPosition! 0
@02:38:53.79 Toroid.seek :home
@02:38:53.87 Toroid.in :default
@02:38:53.99 <LRAUV> Toroid.seek :clear
@02:38:55.61 Carousel.in :default
@02:38:55.67 Carousel.home.jog 0
@02:38:56.17 Carousel.in :default
@02:38:56.23 Carousel.seek 19
@02:38:56.39 Selecting Cartridge 22
@02:38:56.62 Carousel.seek 22
@02:38:57.52 Lysis1.in :fast
@02:38:57.58 Lysis1.seek :locked
@02:39:00.18 <Plunge> Plunge.seek :locked
@02:39:00.32 <Air> Air.in :fast
@02:39:00.38 Air.seek :locked
@02:39:02.98 <LRAUV> Powering cartridge bus
@02:39:05.10 Gate.setIR 1750,5000,0,6
@02:39:05.23 Gate.setIR 0,5000,0,6
@02:39:05.28 Gate.power :cartridge,:OFF
@02:39:05.81 Mounted archive Cartridge
@02:39:24.30 -> Cmd.startFiltering
@02:39:24.76 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@02:39:24.78 SamplePump.setPosition! 0ml
@02:39:24.83 Bypass.open
@02:39:26.29 Intake.open
@02:39:27.07 Sea 4.5m deep (21.3psia)
@02:39:27.09 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@02:39:29.66 Vacuum tare is 51% of full scale
@02:39:29.79 Pressure.in :default
@02:39:29.84 Pressure.hold
@02:39:30.01 SamplePump.in :prime
@02:39:30.08 SamplePump.seek 10ml,1:21
@02:39:39.47 Pressure.coast
@02:39:39.52 SamplePump.setPosition! 0ml
@02:39:39.57 Exhaust.open
@02:39:40.99 <closeBypass> Bypass.close
@02:39:42.54 <FILTERING> Pressure.in :default
@02:39:42.60 Pressure.hold
@02:39:42.71 Sea 3.9m deep (20.5psia)
@02:39:42.84 SamplePump.in :prime
@02:39:42.90 SamplePump.seek 100ml,8:51
@02:41:53.34 Pressure.coast
@02:41:53.39 Bypass.open
@02:41:54.11 Intake.close
@02:41:54.94 Toroid.in :hold
@02:41:55.00 Toroid.seek :crack
@02:41:56.57 Toroid.seek :cartridge
@02:41:57.66 Toroid.in :default
@02:41:57.72 Toroid.seek :clear
@02:41:59.68 Pressurizing cartridge to 26psi above 21.9psia
@02:41:59.69 SamplePump.setPosition! 0ml
@02:41:59.74 Intake.open
@02:42:00.47 Bypass.close
@02:42:01.18 Exhaust.close
@02:42:01.95 Pressure.in :default
@02:42:02.01 Pressure.hold
@02:42:02.17 SamplePump.in :charge
@02:42:02.24 SamplePump.seek 2.3ml,42 seconds
@02:42:08.51 Pressure.coast
@02:42:08.56 Intake.close
@02:42:09.40 Pressurized cartridge with 0.172ml to 26.3psi
@02:42:09.41 Isolated @41.8psia (19.9psi)
@02:42:09.42 Verifying cartridge holds at least 0.75..0.85 of 41.8psia
@02:42:09.45 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@02:42:14.07 Sampling 1000.0ml
@02:42:14.09 SamplePump.setPosition! 0ml
@02:42:14.14 Exhaust.open
@02:42:14.86 Intake.open
@02:42:15.71 Pressure.in :default
@02:42:15.77 Pressure.hold
@02:42:15.89 Sea 4.1m deep (20.7psia)
@02:42:16.02 SamplePump.in :sample
@02:42:16.09 SamplePump.seek 1000ml,1:23:53
@02:42:18.76 <sampling> Cartridge 22 filtering 1/1000ml at 0.28ml/s with 24.9psi vacuum
@02:43:18.75 Cartridge 22 filtering 20/1000ml at 0.32ml/s with 21.4psi vacuum 4.5m deep (21.3psia)
@02:44:18.75 Cartridge 22 filtering 39/1000ml at 0.35ml/s with 20.8psi vacuum 5.9m deep (23.4psia)
@02:45:18.75 Cartridge 22 filtering 58/1000ml at 0.32ml/s with 22.8psi vacuum 2.1m deep (17.8psia)
@02:46:18.75 Cartridge 22 filtering 76/1000ml at 0.30ml/s with 20.3psi vacuum 5.4m deep (22.6psia)
@02:47:18.75 Cartridge 22 filtering 95/1000ml at 0.35ml/s with 23.2psi vacuum 4.3m deep (21.0psia)
@02:48:18.75 Cartridge 22 filtering 113/1000ml at 0.35ml/s with 25.5psi vacuum 3.0m deep (19.2psia)
@02:49:18.75 Cartridge 22 filtering 131/1000ml at 0.29ml/s with 28.0psi vacuum 5.8m deep (23.3psia)
@02:50:18.75 Cartridge 22 filtering 149/1000ml at 0.34ml/s with 23.4psi vacuum 3.8m deep (20.3psia)
@02:51:18.75 Cartridge 22 filtering 166/1000ml at 0.26ml/s with 18.5psi vacuum 3.0m deep (19.1psia)
@02:52:18.75 Cartridge 22 filtering 184/1000ml at 0.33ml/s with 24.5psi vacuum 7.1m deep (25.2psia)
@02:53:18.75 Cartridge 22 filtering 201/1000ml at 0.28ml/s with 28.1psi vacuum 2.7m deep (18.7psia)
@02:54:18.75 Cartridge 22 filtering 219/1000ml at 0.29ml/s with 26.9psi vacuum 3.2m deep (19.4psia)
@02:55:18.75 Cartridge 22 filtering 236/1000ml at 0.24ml/s with 22.2psi vacuum 4.7m deep (21.7psia)
@02:56:18.75 Cartridge 22 filtering 253/1000ml at 0.25ml/s with 19.3psi vacuum 1.9m deep (17.5psia)
@02:57:18.75 Cartridge 22 filtering 270/1000ml at 0.24ml/s with 26.3psi vacuum 2.4m deep (18.2psia)
@02:58:18.75 Cartridge 22 filtering 287/1000ml at 0.30ml/s with 21.9psi vacuum 5.7m deep (23.1psia)
@02:59:18.75 Cartridge 22 filtering 303/1000ml at 0.30ml/s with 20.5psi vacuum 3.4m deep (19.7psia)
@03:00:18.75 Cartridge 22 filtering 320/1000ml at 0.29ml/s with 24.7psi vacuum 4.1m deep (20.8psia)
@03:01:18.75 Cartridge 22 filtering 337/1000ml at 0.24ml/s with 21.4psi vacuum 4.4m deep (21.2psia)
@03:02:18.75 Cartridge 22 filtering 353/1000ml at 0.30ml/s with 23.7psi vacuum 2.6m deep (18.5psia)
@03:03:18.75 Cartridge 22 filtering 370/1000ml at 0.29ml/s with 25.3psi vacuum 4.8m deep (21.7psia)
@03:04:18.75 Cartridge 22 filtering 386/1000ml at 0.28ml/s with 21.9psi vacuum 4.3m deep (21.1psia)
@03:05:18.75 Cartridge 22 filtering 402/1000ml at 0.25ml/s with 28.1psi vacuum 1.6m deep (17.1psia)
@03:06:18.75 Cartridge 22 filtering 418/1000ml at 0.23ml/s with 20.8psi vacuum 5.8m deep (23.2psia)
@03:07:18.75 Cartridge 22 filtering 435/1000ml at 0.28ml/s with 22.3psi vacuum 3.4m deep (19.7psia)
@03:08:18.75 Cartridge 22 filtering 451/1000ml at 0.23ml/s with 22.5psi vacuum 1.9m deep (17.6psia)
@03:09:18.75 Cartridge 22 filtering 467/1000ml at 0.30ml/s with 23.8psi vacuum 5.8m deep (23.3psia)
@03:10:18.75 Cartridge 22 filtering 482/1000ml at 0.22ml/s with 22.3psi vacuum 2.7m deep (18.8psia)
@03:11:18.75 Cartridge 22 filtering 498/1000ml at 0.21ml/s with 21.9psi vacuum 3.1m deep (19.3psia)
@03:12:18.75 Cartridge 22 filtering 514/1000ml at 0.27ml/s with 27.7psi vacuum 4.2m deep (20.8psia)
@03:13:18.75 Cartridge 22 filtering 530/1000ml at 0.28ml/s with 25.3psi vacuum 2.1m deep (17.8psia)
@03:14:18.75 Cartridge 22 filtering 545/1000ml at 0.29ml/s with 23.3psi vacuum 4.2m deep (21.0psia)
@03:15:18.75 Cartridge 22 filtering 561/1000ml at 0.29ml/s with 23.2psi vacuum 5.4m deep (22.7psia)
@03:16:18.75 Cartridge 22 filtering 576/1000ml at 0.28ml/s with 23.6psi vacuum 2.4m deep (18.3psia)
@03:17:18.75 Cartridge 22 filtering 592/1000ml at 0.25ml/s with 26.7psi vacuum 4.8m deep (21.8psia)
@03:18:18.75 Cartridge 22 filtering 607/1000ml at 0.21ml/s with 21.7psi vacuum 4.2m deep (20.9psia)
@03:19:18.75 Cartridge 22 filtering 622/1000ml at 0.22ml/s with 27.1psi vacuum
@03:20:18.75 Cartridge 22 filtering 637/1000ml at 0.27ml/s with 23.6psi vacuum 5.5m deep (22.8psia)
@03:21:18.75 Cartridge 22 filtering 652/1000ml at 0.29ml/s with 24.4psi vacuum 4.7m deep (21.6psia)
@03:22:18.75 Cartridge 22 filtering 667/1000ml at 0.28ml/s with 24.1psi vacuum 2.8m deep (18.8psia)
@03:23:18.75 Cartridge 22 filtering 682/1000ml at 0.26ml/s with 24.1psi vacuum 4.9m deep (21.9psia)
@03:24:18.75 Cartridge 22 filtering 696/1000ml at 0.24ml/s with 27.9psi vacuum
@03:25:18.75 Cartridge 22 filtering 711/1000ml at 0.23ml/s with 26.3psi vacuum 2.8m deep (18.8psia)
@03:26:18.75 Cartridge 22 filtering 725/1000ml at 0.26ml/s with 25.6psi vacuum 5.4m deep (22.7psia)
@03:27:18.75 Cartridge 22 filtering 740/1000ml at 0.22ml/s with 27.5psi vacuum 1.7m deep (17.2psia)
@03:28:18.75 Cartridge 22 filtering 754/1000ml at 0.23ml/s with 27.8psi vacuum 3.1m deep (19.3psia)
@03:29:18.75 Cartridge 22 filtering 768/1000ml at 0.25ml/s with 24.0psi vacuum 4.3m deep (21.0psia)
@03:30:18.75 Cartridge 22 filtering 782/1000ml at 0.23ml/s with 26.4psi vacuum 1.6m deep (17.0psia)
@03:31:18.75 Cartridge 22 filtering 796/1000ml at 0.24ml/s with 20.8psi vacuum 5.1m deep (22.2psia)
@03:32:18.75 Cartridge 22 filtering 810/1000ml at 0.20ml/s with 25.7psi vacuum 1.6m deep (17.1psia)
@03:33:18.75 Cartridge 22 filtering 824/1000ml at 0.24ml/s with 24.3psi vacuum 2.7m deep (18.7psia)
@03:34:18.75 Cartridge 22 filtering 837/1000ml at 0.20ml/s with 25.3psi vacuum 4.1m deep (20.8psia)
@03:35:18.75 Cartridge 22 filtering 851/1000ml at 0.23ml/s with 23.2psi vacuum 3.2m deep (19.4psia)
@03:36:18.75 Cartridge 22 filtering 864/1000ml at 0.25ml/s with 24.7psi vacuum 4.2m deep (21.0psia)
@03:37:18.75 Cartridge 22 filtering 878/1000ml at 0.22ml/s with 22.9psi vacuum 5.5m deep (22.8psia)
@03:38:18.75 Cartridge 22 filtering 891/1000ml at 0.23ml/s with 27.8psi vacuum 1.9m deep (17.5psia)
@03:39:18.75 Cartridge 22 filtering 904/1000ml at 0.20ml/s with 24.1psi vacuum 3.9m deep (20.5psia)
@03:40:18.75 Cartridge 22 filtering 917/1000ml at 0.20ml/s with 27.7psi vacuum 2.8m deep (18.9psia)
@03:41:18.75 Cartridge 22 filtering 930/1000ml at 0.20ml/s with 24.8psi vacuum 1.8m deep (17.3psia)
@03:42:18.75 Cartridge 22 filtering 943/1000ml at 0.22ml/s with 25.6psi vacuum 5.3m deep (22.5psia)
@03:43:18.75 Cartridge 22 filtering 956/1000ml at 0.20ml/s with 26.0psi vacuum 3.4m deep (19.8psia)
@03:44:18.75 Cartridge 22 filtering 969/1000ml at 0.20ml/s with 25.1psi vacuum 2.7m deep (18.7psia)
@03:45:18.75 Cartridge 22 filtering 982/1000ml at 0.20ml/s with 25.7psi vacuum 5.1m deep (22.2psia)
@03:46:18.75 Cartridge 22 filtering 994/1000ml at 0.20ml/s with 28.8psi vacuum
@03:46:45.61 <FILTERING> Pressure.coast
@03:46:45.73 <closeIntake> Intake.close
@03:46:45.77 <closeExhaust> Exhaust.close
@03:46:45.93 <FILTERING> Toroid.in :hold
@03:46:46.00 Toroid.seek :bypass
@03:46:47.61 Toroid.in :default
@03:46:47.67 Toroid.seek :clear
@03:46:49.28 Sampled  1000.0ml
@03:46:50.93 <LRAUV> -> Cmd.startProcessing
@03:46:51.31 <PROCESSING> Plunge.seek :reagent
@03:46:52.15 Sample loop closed. Ready to Deliver Reagents
@03:46:52.16 Archive.seek :sealed
@03:46:56.42 Archive.seek :evac
@03:47:10.35 delay 1:00
@03:48:10.38 Archive.seek :clear
@03:48:28.09 RNAlater soak for 10:00
@03:58:28.12 Archive.seek :tVent
@03:58:30.70 delay 1 second
@03:58:31.72 Archive.seek :evac
@03:58:47.34 delay 10 seconds
@03:58:57.37 Sample preserved and evacuated
@03:58:58.57 <LRAUV> -> Cmd.stop
@03:58:58.71 Carousel.seek 22
@03:58:58.89 Plunge.seek :locked
@03:58:59.83 <Archive> Archive.in :unlocking
@03:58:59.95 Archive.seek :clear
@03:58:59.99 <Air> Air.in :unlocking
@03:59:00.14 <Lysis1> Lysis1.in :unlocking
@03:59:00.18 <Air> Air.seek :clear
@03:59:00.29 <Lysis1> Lysis1.seek :clear
@03:59:04.03 <LRAUV> Archive.coast
@03:59:04.50 Air.coast
@03:59:04.55 Lysis1.coast
@03:59:04.60 Lysis2.coast
@03:59:04.65 Plunge.coast
@03:59:04.70 SamplePump.coast
@03:59:04.75 Toroid.coast
@03:59:04.80 Carousel.coast
@03:59:04.86 PinPort.coast
@03:59:05.04 Gate.power :motor,:OFF
@03:59:05.06 Gate.power :core,:OFF
@03:59:05.08 Gate.power :analytic1,:OFF
@03:59:05.10 Gate.power :analytic2,:OFF
@03:59:05.12 Gate.power :analytic3,:OFF
@03:59:05.14 Gate.power :main,:OFF
@03:59:05.16 Safely stopped and ready to power off
##-- file closed 2021-04-24T11:11:02.097Z

##-- file opened 2021-04-24T11:11:02.099Z
@04:11:01.98 -> showStatus
@04:11:04.00 -> Cmd.loadCartridge -9
@04:11:04.14 Gate.power :main,:ON,limit to 5000mA
@04:11:04.16 Gate.power :analytic1,:ON,limit to 5000mA
@04:11:04.18 Gate.power :analytic2,:ON,limit to 5000mA
@04:11:04.21 Gate.power :analytic3,:ON,limit to 5000mA
@04:11:04.34 Powering core bus
@04:11:06.62 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@04:11:06.75 <LRAUV> Archive.configure :default
@04:11:06.88 Air.configure :default
@04:11:06.98 Lysis1.configure :default
@04:11:07.08 Lysis2.configure :default
@04:11:07.55 Plunge.configure :default
@04:11:07.68 SamplePump.configure :default
@04:11:07.82 ExtraPump.configure :default
@04:11:07.93 Toroid.configure :default
@04:11:08.05 Carousel.configure :default
@04:11:08.18 Pressure.configure :default
@04:11:08.29 PinPort.configure :default
@04:11:08.36 All elf controllers running firmware version 4.741
@04:11:08.39 Gate.power :motor,:ON,limit to 5000mA
@04:11:08.69 <Lysis1_home> Lysis1.in :default
@04:11:08.73 <Archive_home> Archive.in :default
@04:11:08.78 <Air_home> Air.in :default
@04:11:09.02 <Lysis1_home> Lysis1.home.jog 0
@04:11:09.06 <Archive_home> Archive.home.jog 0
@04:11:09.09 <Air_home> Air.home.jog 0
@04:11:09.31 <Toroid_home> Toroid.in :default
@04:11:09.38 <Plunge_home> Plunge.in :default
@04:11:09.42 <Lysis2_home> Lysis2.in :default
@04:11:09.46 <PinPort_home> PinPort.in :default
@04:11:09.70 <Toroid_home> Toroid.home.jog 0
@04:11:09.73 <Plunge_home> Plunge.home.jog 0
@04:11:09.76 <PinPort_home> PinPort.home.jog 0
@04:11:09.79 <Lysis2_home> Lysis2.home.jog 0
@04:11:10.10 <Air_home> Air.in :homing
@04:11:10.15 <Lysis1_home> Lysis1.in :homing
@04:11:10.23 <Archive_home> Archive.in :homing
@04:11:10.35 <Air_home> Air.jog 156
@04:11:10.42 <Lysis1_home> Lysis1.jog 1416
@04:11:10.46 <Archive_home> Archive.jog 159
@04:11:10.65 <Toroid_home> Toroid.in :homing
@04:11:10.67 <Plunge_home> Plunge.in :homing
@04:11:10.70 <PinPort_home> PinPort.in :homing
@04:11:10.75 <Lysis2_home> Lysis2.in :homing
@04:11:10.86 <PinPort_home> PinPort.jog -107
@04:11:10.90 <Plunge_home> Plunge.jog 1580
@04:11:10.93 <Toroid_home> Toroid.jog 618
@04:11:11.05 <Lysis2_home> Lysis2.jog 1431
@04:11:11.18 <Air_home> Air.setRawPosition! 50
@04:11:11.20 <PinPort_home> PinPort.setRawPosition! 0
@04:11:11.23 <Archive_home> Archive.setRawPosition! 50
@04:11:11.32 <Air_home> Air.seek :home
@04:11:11.39 <PinPort_home> PinPort.seek :home
@04:11:11.44 <Archive_home> Archive.seek :home
@04:11:11.59 <PinPort_home> PinPort.in :default
@04:11:11.71 <Lysis1_home> Lysis1.setRawPosition! 50
@04:11:11.73 <Air_home> Air.in :default
@04:11:11.82 <Lysis1_home> Lysis1.seek :home
@04:11:11.92 <Archive_home> Archive.in :default
@04:11:12.11 <Lysis1_home> Lysis1.in :default
@04:11:12.24 <Plunge_home> Plunge.setRawPosition! 50
@04:11:12.29 Plunge.seek :home
@04:11:12.37 <Lysis2_home> Lysis2.setRawPosition! 50
@04:11:12.42 Lysis2.seek :home
@04:11:12.57 <Plunge_home> Plunge.in :default
@04:11:12.70 <Lysis2_home> Lysis2.in :default
@04:11:13.12 <Toroid_home> Toroid.setRawPosition! 0
@04:11:13.17 Toroid.seek :home
@04:11:13.25 Toroid.in :default
@04:11:13.37 <LRAUV> Toroid.seek :clear
@04:11:14.99 Carousel.in :default
@04:11:15.05 Carousel.home.jog 0
@04:11:15.54 Carousel.in :default
@04:11:15.60 Carousel.seek 22
@04:11:15.73 Selecting Cartridge 17
@04:11:15.97 Carousel.seek 17
@04:11:17.28 Lysis1.in :fast
@04:11:17.35 Lysis1.seek :locked
@04:11:19.94 <Plunge> Plunge.seek :locked
@04:11:20.08 <Air> Air.in :fast
@04:11:20.14 Air.seek :locked
@04:11:22.73 <LRAUV> Powering cartridge bus
@04:11:24.85 Gate.setIR 1750,5000,0,6
@04:11:24.98 Gate.setIR 0,5000,0,6
@04:11:25.04 PuckHeater.configure :default
@04:11:25.09 Gate.power :heat,:ON,limit to 5000mA
@04:11:25.76 Mounted lysis Cartridge board#Q05045
@04:11:25.79 Using default puck heat loss curve for HeatingAccelInCarousel
@04:11:44.40 -> Cmd.startFiltering
@04:11:44.85 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@04:11:44.86 SamplePump.setPosition! 0ml
@04:11:44.90 Bypass.open
@04:11:46.39 Intake.open
@04:11:47.18 Sea 4.9m deep (21.9psia)
@04:11:47.20 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@04:11:49.77 Vacuum tare is 51% of full scale
@04:11:49.90 Pressure.in :default
@04:11:49.96 Pressure.hold
@04:11:50.19 SamplePump.in :prime
@04:11:50.25 SamplePump.seek 10ml,1:21
@04:11:59.64 Pressure.coast
@04:11:59.69 SamplePump.setPosition! 0ml
@04:11:59.74 Exhaust.open
@04:12:01.15 <closeBypass> Bypass.close
@04:12:02.70 <FILTERING> Pressure.in :default
@04:12:02.76 Pressure.hold
@04:12:02.87 Sea 6.2m deep (23.8psia)
@04:12:03.00 SamplePump.in :prime
@04:12:03.06 SamplePump.seek 100ml,8:51
@04:14:13.36 Pressure.coast
@04:14:13.42 Bypass.open
@04:14:14.13 Intake.close
@04:14:14.98 Toroid.in :hold
@04:14:15.04 Toroid.seek :crack
@04:14:16.59 Toroid.seek :cartridge
@04:14:17.69 Toroid.in :default
@04:14:17.75 Toroid.seek :clear
@04:14:19.71 Pressurizing cartridge to 26psi above 21.7psia
@04:14:19.72 SamplePump.setPosition! 0ml
@04:14:19.76 Intake.open
@04:14:20.50 Bypass.close
@04:14:21.20 Exhaust.close
@04:14:21.98 Pressure.in :default
@04:14:22.04 Pressure.hold
@04:14:22.21 SamplePump.in :charge
@04:14:22.27 SamplePump.seek 2.3ml,42 seconds
@04:14:31.85 Pressure.coast
@04:14:31.90 Intake.close
@04:14:33.17 Pressurized cartridge with 0.409ml to 26.3psi
@04:14:33.19 Isolated @44.7psia (22.9psi)
@04:14:33.20 Verifying cartridge holds at least 0.75..0.85 of 44.7psia
@04:14:33.24 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@04:14:37.86 Sampling 1000.0ml
@04:14:37.88 SamplePump.setPosition! 0ml
@04:14:37.93 Exhaust.open
@04:14:38.63 Intake.open
@04:14:39.49 Pressure.in :default
@04:14:39.55 Pressure.hold
@04:14:39.66 Sea 6.5m deep (24.3psia)
@04:14:39.80 SamplePump.in :sample
@04:14:39.87 SamplePump.seek 1000ml,1:23:53
@04:14:42.53 <sampling> Cartridge 17 filtering 1/1000ml at 0.30ml/s with 21.6psi vacuum 4.2m deep (20.9psia)
@04:15:42.52 Cartridge 17 filtering 18/1000ml at 0.31ml/s with 21.8psi vacuum 5.1m deep (22.2psia)
@04:16:42.52 Cartridge 17 filtering 34/1000ml at 0.25ml/s with 22.9psi vacuum 2.2m deep (17.9psia)
@04:17:42.51 Cartridge 17 filtering 50/1000ml at 0.30ml/s with 22.2psi vacuum 4.8m deep (21.8psia)
@04:18:42.52 Cartridge 17 filtering 66/1000ml at 0.28ml/s with 22.1psi vacuum 2.8m deep (18.8psia)
@04:19:42.52 Cartridge 17 filtering 82/1000ml at 0.29ml/s with 25.2psi vacuum 3.2m deep (19.4psia)
@04:20:42.52 Cartridge 17 filtering 98/1000ml at 0.26ml/s with 20.6psi vacuum 4.9m deep (21.9psia)
@04:21:42.51 Cartridge 17 filtering 113/1000ml at 0.26ml/s with 22.2psi vacuum 4.5m deep (21.3psia)
@04:22:42.52 Cartridge 17 filtering 129/1000ml at 0.22ml/s with 22.5psi vacuum
@04:23:42.52 Cartridge 17 filtering 144/1000ml at 0.21ml/s with 21.7psi vacuum 5.2m deep (22.4psia)
@04:24:42.52 Cartridge 17 filtering 159/1000ml at 0.25ml/s with 22.1psi vacuum 3.8m deep (20.4psia)
@04:25:42.52 Cartridge 17 filtering 174/1000ml at 0.21ml/s with 22.0psi vacuum 1.9m deep (17.6psia)
@04:26:42.52 Cartridge 17 filtering 189/1000ml at 0.22ml/s with 21.1psi vacuum 5.7m deep (23.1psia)
@04:27:42.52 Cartridge 17 filtering 203/1000ml at 0.27ml/s with 22.0psi vacuum 3.6m deep (20.1psia)
@04:28:42.52 Cartridge 17 filtering 217/1000ml at 0.24ml/s with 22.9psi vacuum
@04:29:42.52 Cartridge 17 filtering 231/1000ml at 0.26ml/s with 23.5psi vacuum 6.1m deep (23.7psia)
@04:30:42.52 Cartridge 17 filtering 245/1000ml at 0.23ml/s with 21.8psi vacuum 2.4m deep (18.3psia)
@04:31:42.52 Cartridge 17 filtering 259/1000ml at 0.23ml/s with 22.3psi vacuum 4.3m deep (21.1psia)
@04:32:42.52 Cartridge 17 filtering 272/1000ml at 0.20ml/s with 27.2psi vacuum 4.0m deep (20.6psia)
@04:33:42.52 Cartridge 17 filtering 286/1000ml at 0.20ml/s with 24.9psi vacuum 1.8m deep (17.4psia)
@04:34:42.52 Cartridge 17 filtering 299/1000ml at 0.24ml/s with 23.2psi vacuum 4.3m deep (21.1psia)
@04:35:42.52 Cartridge 17 filtering 312/1000ml at 0.23ml/s with 25.3psi vacuum 4.4m deep (21.3psia)
@04:36:42.52 Cartridge 17 filtering 325/1000ml at 0.24ml/s with 23.9psi vacuum 2.5m deep (18.3psia)
@04:37:42.52 Cartridge 17 filtering 338/1000ml at 0.21ml/s with 23.4psi vacuum 4.7m deep (21.6psia)
@04:38:42.52 Cartridge 17 filtering 351/1000ml at 0.22ml/s with 23.4psi vacuum 4.5m deep (21.3psia)
@04:39:42.52 Cartridge 17 filtering 364/1000ml at 0.21ml/s with 23.6psi vacuum 1.6m deep (17.1psia)
@04:40:42.52 Cartridge 17 filtering 376/1000ml at 0.20ml/s with 25.0psi vacuum 5.3m deep (22.5psia)
@04:41:42.52 Cartridge 17 filtering 389/1000ml at 0.20ml/s with 25.8psi vacuum 3.5m deep (19.9psia)
@04:42:42.52 Cartridge 17 filtering 401/1000ml at 0.20ml/s with 26.4psi vacuum 1.8m deep (17.4psia)
@04:43:42.52 Cartridge 17 filtering 414/1000ml at 0.22ml/s with 25.5psi vacuum 5.3m deep (22.5psia)
@04:44:42.52 Cartridge 17 filtering 426/1000ml at 0.20ml/s with 27.6psi vacuum 2.2m deep (17.9psia)
@04:45:42.52 Cartridge 17 filtering 438/1000ml at 0.20ml/s with 24.7psi vacuum 2.4m deep (18.3psia)
@04:46:42.52 Cartridge 17 filtering 450/1000ml at 0.20ml/s with 24.5psi vacuum 4.3m deep (21.1psia)
@04:47:42.52 Cartridge 17 filtering 462/1000ml at 0.20ml/s with 25.2psi vacuum
@04:48:42.05 <FILTERING> Flow averaged 0.23ml/s over 34:03
@04:48:42.08 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi
@04:48:42.51 <sampling> Cartridge 17 filtering 475/1000ml at 0.00ml/s with 8.0psi vacuum 4.2m deep (20.9psia)
@04:48:42.65 <FILTERING> Relieved excess vacuum in .6 seconds
@04:48:42.68 SamplePump.seek 1000ml,44:19
@04:48:56.43 Flow averaged 0.20ml/s over 14 seconds
@04:48:56.46 Waiting up to 11 seconds for vacuum to reduce from 27.4 to 18.0psi
@04:48:57.03 Relieved excess vacuum in .6 seconds
@04:48:57.06 SamplePump.seek 1000ml,44:05
@04:49:10.72 Flow averaged 0.20ml/s over 14 seconds
@04:49:10.74 <closeIntake> Intake.close
@04:49:10.80 <closeExhaust> Exhaust.close
@04:49:10.98 <FILTERING> Toroid.in :hold
@04:49:11.04 Toroid.seek :bypass
@04:49:12.66 Toroid.in :default
@04:49:12.72 Toroid.seek :clear
@04:49:14.33 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@04:49:14.34 Pressure.coast
@04:49:14.52 Sampled  480.0ml
@04:49:16.41 <LRAUV> -> Cmd.startProcessing
@04:49:17.85 <PROCESSING> SPR.command \"read_sequences\"
@04:49:18.26 SPR.command \"stats_on\"
@04:49:18.30 SPR.command \"start 0 1\"
@04:49:20.31 <SPRlogger> Begun log /var/log/esp/spr21apr2404h.spr
@04:49:21.11 <PROCESSING> Handoff.select :waste
@04:49:27.79 Syringe tare is 20% of full scale
@04:49:27.81 PinPort.in :locking
@04:49:27.88 PinPort.seek :locked
@04:49:28.35 <posArchive> Archive.in :fast
@04:49:28.41 <posLyS2> Lysis2.in :fast
@04:49:28.44 <PROCESSING> Lysis1.seek 1350uL
@04:49:28.56 <posArchive> Archive.seek :full
@04:49:28.61 <posLyS2> Lysis2.seek :full
@04:49:30.70 <PROCESSING> Plunge.seek :reagent
@04:49:31.60 Evacuating puck with filter bubble point of 50psi
@04:49:31.62 PinPort.seek :locked
@04:49:31.70 Handoff.select :waste
@04:49:38.28 Air syringe pumping up to 1 stroke to reach 8psi
@04:49:38.29 Air stroke #1 of 1
@04:49:38.37 Air.in :pressurize
@04:49:38.43 Air.seek :pressurized
@04:49:49.69 -0.1psi after pressurize
@04:49:49.70 Air.in :compress
@04:49:49.77 Air.seek :evac
@04:49:53.85 0.4psi after compress
@04:50:06.42 0.1psi after compress (stable)
@04:50:06.44 Air.in :default
@04:50:06.51 Air.hold
@04:50:06.68 at 0.1psi Opened to waste
@04:50:06.69 Handoff.select :blocked
@04:50:06.82 Archive syringe pumping up to 5 strokes to reach 50psi
@04:50:06.83 Archive stroke #1 of 5
@04:50:06.91 Archive.in :pressurize
@04:50:06.98 Archive.seek :pressurized
@04:50:19.72 6.8psi after pressurize
@04:50:19.73 Archive.in :compress
@04:50:19.81 Archive.seek :empty
@04:50:35.70 32.5psi after compress
@04:51:01.27 47.4psi after compress (stable)
@04:51:01.29 Archive.in :gulp
@04:51:01.36 Archive.seek :topVent
@04:51:08.56 48.6psi after gulp
@04:51:21.13 48.4psi after gulp (stable)
@04:51:21.14 Archive stroke #2 of 5
@04:51:21.23 Archive.in :pressurize
@04:51:21.29 Archive.seek :pressurized
@04:51:34.04 48.1psi after pressurize
@04:51:34.06 Archive.in :compress
@04:51:34.13 Archive.seek :empty
@04:51:45.25 Reached 50.2 of 50.0psi after pumping 3359uL (1.94 strokes)
@04:51:45.26 Archive.in :default
@04:51:45.34 Archive.hold
@04:51:45.42 Handoff.select :waste
@04:51:58.10 delay 1:00
@04:52:58.13 Handoff.select :blocked
@04:53:20.85 Handoff.select :waste
@04:53:20.99 -0.0psi at evacEnd
@04:53:21.00 Handoff.select :blocked
@04:53:21.12 Archive.in :fast
@04:53:21.19 Archive.seek :topVent
@04:53:34.59 Stable @0.2psi after Handoff blocked
@04:53:34.62 Archive syringe pumping up to 5 strokes to reach 30psi
@04:53:34.63 Archive stroke #1 of 5
@04:53:34.72 Archive.in :pressurize
@04:53:34.79 Archive.seek :pressurized
@04:53:47.51 0.3psi after pressurize
@04:53:47.52 Archive.in :compress
@04:53:47.60 Archive.seek :empty
@04:54:03.50 0.6psi after compress
@04:55:14.07 12.4psi after compress (stable)
@04:55:14.09 Archive.in :gulp
@04:55:14.16 Archive.seek :topVent
@04:55:21.36 13.9psi after gulp
@04:55:33.93 15.1psi after gulp (stable)
@04:55:33.94 Archive stroke #2 of 5
@04:55:34.03 Archive.in :pressurize
@04:55:34.09 Archive.seek :pressurized
@04:55:46.82 16.3psi after pressurize
@04:55:46.84 Archive.in :compress
@04:55:46.91 Archive.seek :empty
@04:56:02.79 17.2psi after compress
@04:56:29.11 Reached 30.3 of 30.2psi after pumping 3460uL (2.00 strokes)
@04:56:29.13 Archive.in :default
@04:56:29.20 Archive.hold
@04:56:29.26 Handoff.select :waste
@04:56:39.49 delay 1:00
@04:57:39.52 Handoff.select :blocked
@04:58:05.71 Handoff.select :waste
@04:58:05.83 3.7psi at evacEnd
@04:58:05.85 Air.seek :fullVent
@04:58:07.29 Archive.in :fast
@04:58:07.35 Archive.seek :topVent
@04:58:14.49 Seawater evacuated, Presure above/below filter released. Lysis begins
@04:58:14.58 Lysis1.in :slow
@04:58:14.64 Lysis1.seek 1050uL
@04:58:44.67 delay 5 seconds
@04:58:49.69 Handoff.select :blocked
@04:58:49.85 Lysis1.seek 450uL
@04:59:49.79 delay 3 seconds
@04:59:52.86 Air.in to mix into 1.37806 parts Lysis1
@04:59:53.41 <Airmixer> Air.seek :evac
@04:59:53.45 <PROCESSING> Lysis1.seek 300uL
@05:00:08.56 Lysis1.seek :empty
@05:00:38.66 Lysis buffer delivered @25.2psi
@05:00:38.67 Polling Can every 2:00
@05:00:39.22 PuckHeater.configure :default #ambient 11.3C
@05:00:39.26 PuckHeater.seek 80.2C
@05:01:45.60 PuckHeater.hold 2:00,80.2C
@05:03:45.37 Polling Can every 2:00
@05:03:45.92 PuckHeater.configure :default #ambient 11.4C
@05:03:45.97 PuckHeater.hold 30 seconds,79.2C
@05:04:16.09 Polling Can every 2:00
@05:04:16.64 PuckHeater.hold 30 seconds,78.7C
@05:04:46.75 Polling Can every 2:00
@05:04:47.30 PuckHeater.configure :default #ambient 11.4C
@05:04:47.35 PuckHeater.hold 10:00,78.1C
@05:14:46.03 Lysis complete. Syringe pressure @33.3psi
@05:14:46.04 Handoff.select :blocked
@05:14:46.08 Archive syringe pumping up to 3 strokes to reach 40psi
@05:14:46.09 Archive stroke #1 of 3
@05:14:46.18 Archive.in :pressurize
@05:14:46.24 Archive.seek :pressurized
@05:14:58.99 33.0psi after pressurize
@05:14:59.00 Archive.in :compress
@05:14:59.07 Archive.seek :empty
@05:15:07.83 Reached 41.1 of 40.0psi after pumping 1578uL (0.91 strokes)
@05:15:07.84 Archive.in :default
@05:15:07.91 Archive.hold
@05:15:07.99 delay 2 seconds
@05:15:10.03 Archive.in :fast
@05:15:10.09 Archive.seek :full
@05:15:16.76 Lysis1.seek 138uL
@05:15:30.64 delay 1 second
@05:15:31.94 <fillArchive> Archive.seek :full
@05:15:31.96 <PROCESSING> Lysis1.seek :empty
@05:15:45.93 Initial air pressure @45.7psi
@05:15:45.99 Lysis1.in :extract
@05:15:46.08 Lysis1.seek 600uL,nil
@05:15:47.75 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@05:15:47.76 Archive stroke #1 of 6
@05:15:47.90 Archive.in :pressurize
@05:15:47.96 Archive.seek :pressurized
@05:16:00.69 16.0psi after pressurize
@05:16:00.70 Archive.in :compress
@05:16:00.77 Archive.seek :empty
@05:16:16.65 15.9psi after compress
@05:16:18.72 Archive.in :default
@05:16:18.77 Archive.hold
@05:16:18.89 <PROCESSING> Initial 600uL lysate @16.3psi
@05:16:18.91 Handoff.select :blocked
@05:16:19.01 Initial air pressure @16.9psi
@05:16:19.08 Lysis1.seek 825uL,nil
@05:16:19.72 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@05:16:19.73 Archive stroke #1 of 6
@05:16:19.82 Archive.in :compress
@05:16:19.89 Archive.seek :empty
@05:16:20.07 14.0psi after compress
@05:16:32.69 15.9psi after compress (stable)
@05:16:37.82 Recovering 4.4uL/s
@05:16:42.94 Recovering 2.7uL/s
@05:16:48.00 Recovering 2.5uL/s
@05:16:53.06 Recovering 2.1uL/s
@05:16:58.13 Archive.in :gulp
@05:16:58.21 Archive.seek :topVent
@05:17:05.45 15.7psi after gulp
@05:17:18.02 15.7psi after gulp (stable)
@05:17:18.38 Archive stroke #2 of 6
@05:17:18.59 Archive.in :pressurize
@05:17:18.66 Archive.seek :pressurized
@05:17:31.44 15.8psi after pressurize
@05:17:31.46 Archive.in :compress
@05:17:31.54 Archive.seek :empty
@05:17:41.42 Archive.in :default
@05:17:41.47 Archive.hold
@05:17:41.77 <PROCESSING> Recovered 825uL lysate @16.5psi
@05:17:41.79 Air.in :halfSpeed
@05:17:41.86 Air.seek :fullVent
@05:17:44.13 Puck Top Vented, bottom @5.5psi
@05:17:44.14 Handoff.select :waste
@05:17:44.26 delay 5 seconds
@05:17:49.32 Handoff open to waste, bottom @0.2psi
@05:17:49.34 Air.in :default
@05:17:49.41 Air.seek :evac
@05:17:50.87 Lysis2.in :DAmix
@05:17:51.01 Lysis1.in :DAmix
@05:17:51.47 <Lysis2mixer> Lysis2.seek 1480uL
@05:17:51.51 <PROCESSING> Lysis1.seek 575uL
@05:18:26.57 delay 5 seconds
@05:18:31.59 Handoff.select SPR
@05:18:31.70 SPR.command \"start 2 14\"
@05:18:32.89 delay 10 seconds
@05:18:42.92 SPR.command \"start 1 0\"
@05:18:46.15 Waiting up to 4:00 for SPR to enter state 1
@05:20:48.53 SPR.command \"hold_on\"
@05:20:49.94 SPR Dilution 1 of 1 -- Running Experiment 1
@05:20:50.36 <Lysis2mixer> Lysis2.seek 1180uL
@05:20:50.43 <PROCESSING> Lysis1.seek 275uL
@05:21:32.44 volL1 = 300.0 and volL2 = 300.0
@05:21:32.45 delay 15 seconds
@05:21:47.47 SPR.command \"hold_off\"
@05:21:50.59 Waiting up to 38:00 for SPR to enter state 7
@05:48:34.14 SPR Analysis has Completed
@05:48:34.16 Handoff.select :waste
@05:48:35.07 <SPRlogger> Ended log /var/log/esp/spr21apr2404h.spr
@05:48:35.08 <PROCESSING> SPR.command \"stats_off\"
@05:48:36.69 <LRAUV> -> Cmd.stop
@05:48:36.78 <closeHandoff> Handoff.select :close
@05:48:36.90 <LRAUV> Gate.power :heat,:OFF
@05:48:36.92 Gate.power :cartridge,:OFF
@05:48:37.00 Carousel.seek 17
@05:48:37.17 Plunge.seek :locked
@05:48:38.06 PinPort.in :unlocking
@05:48:38.12 PinPort.seek :clear
@05:48:38.66 <Archive> Archive.in :unlocking
@05:48:38.79 Archive.seek :clear
@05:48:38.87 <Air> Air.in :unlocking
@05:48:38.98 Air.seek :clear
@05:48:39.01 <Lysis1> Lysis1.in :unlocking
@05:48:39.18 Lysis1.seek :clear
@05:48:39.22 <Lysis2> Lysis2.in :unlocking
@05:48:39.32 Lysis2.seek :clear
@05:48:40.47 Slide::Error in LRAUV -- Plunge Valve (PV) positionErr at locked (sensed locked - 53 counts)
@05:48:40.48 <LRAUV> Archive.coast
@05:48:40.55 Air.coast
@05:48:40.62 Lysis1.coast
@05:48:40.68 Lysis2.coast
@05:48:40.74 Plunge.coast
@05:48:40.80 SamplePump.coast
@05:48:40.85 Toroid.coast
@05:48:40.91 Carousel.coast
@05:48:40.97 PinPort.coast
@05:48:41.24 Gate.power :motor,:OFF
@05:48:41.26 Gate.power :core,:OFF
@05:48:41.28 Gate.power :analytic1,:OFF
@05:48:41.30 Gate.power :analytic2,:OFF
@05:48:41.32 Gate.power :analytic3,:OFF
@05:48:41.35 Gate.power :main,:OFF
@05:48:41.37 ready to power off
##-- file closed 2021-04-24T14:56:13.069Z

##-- file opened 2021-04-24T14:56:13.071Z
@07:56:13.84 -> showStatus
@07:56:15.86 -> Cmd.loadCartridge -6
@07:56:16.00 Gate.power :main,:ON,limit to 5000mA
@07:56:16.02 Gate.power :analytic1,:ON,limit to 5000mA
@07:56:16.04 Gate.power :analytic2,:ON,limit to 5000mA
@07:56:16.06 Gate.power :analytic3,:ON,limit to 5000mA
@07:56:16.19 Powering core bus
@07:56:18.47 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@07:56:18.61 <LRAUV> Archive.configure :default
@07:56:18.73 Air.configure :default
@07:56:18.83 Lysis1.configure :default
@07:56:19.28 Lysis2.configure :default
@07:56:19.41 Plunge.configure :default
@07:56:19.53 SamplePump.configure :default
@07:56:19.68 ExtraPump.configure :default
@07:56:19.79 Toroid.configure :default
@07:56:19.91 Carousel.configure :default
@07:56:20.04 Pressure.configure :default
@07:56:20.16 PinPort.configure :default
@07:56:20.22 All elf controllers running firmware version 4.741
@07:56:20.25 Gate.power :motor,:ON,limit to 5000mA
@07:56:20.37 <Archive_home> Archive.in :default
@07:56:20.69 Archive.home.jog 0
@07:56:20.86 <Toroid_home> Toroid.in :default
@07:56:20.94 <Plunge_home> Plunge.in :default
@07:56:20.97 <Lysis1_home> Lysis1.in :default
@07:56:21.01 <Air_home> Air.in :default
@07:56:21.04 <PinPort_home> PinPort.in :default
@07:56:21.09 <Lysis2_home> Lysis2.in :default
@07:56:21.30 <Toroid_home> Toroid.home.jog 0
@07:56:21.33 <Plunge_home> Plunge.home.jog 0
@07:56:21.37 <Lysis1_home> Lysis1.home.jog 0
@07:56:21.41 <Lysis2_home> Lysis2.home.jog 0
@07:56:21.45 <Air_home> Air.home.jog 0
@07:56:21.48 <PinPort_home> PinPort.home.jog 0
@07:56:21.91 <Archive_home> Archive.in :homing
@07:56:22.13 <PinPort_home> PinPort.in :homing
@07:56:22.17 <Toroid_home> Toroid.in :homing
@07:56:22.20 <Lysis2_home> Lysis2.in :homing
@07:56:22.25 <Air_home> Air.in :homing
@07:56:22.28 <Archive_home> Archive.jog 4573
@07:56:22.31 <Lysis1_home> Lysis1.in :homing
@07:56:22.34 <Plunge_home> Plunge.in :homing
@07:56:22.55 <Toroid_home> Toroid.jog 617
@07:56:22.61 <PinPort_home> PinPort.jog -107
@07:56:22.64 <Lysis2_home> Lysis2.jog 392
@07:56:22.68 <Lysis1_home> Lysis1.jog 4740
@07:56:22.72 <Air_home> Air.jog 4901
@07:56:22.75 <Plunge_home> Plunge.jog 1581
@07:56:23.00 <PinPort_home> PinPort.setRawPosition! 0
@07:56:23.05 PinPort.seek :home
@07:56:23.13 PinPort.in :default
@07:56:23.45 <Lysis2_home> Lysis2.setRawPosition! 50
@07:56:23.50 Lysis2.seek :home
@07:56:23.78 Lysis2.in :default
@07:56:24.10 <Plunge_home> Plunge.setRawPosition! 50
@07:56:24.14 Plunge.seek :home
@07:56:24.42 Plunge.in :default
@07:56:24.75 <Toroid_home> Toroid.setRawPosition! 0
@07:56:24.79 Toroid.seek :home
@07:56:24.90 Toroid.in :default
@07:56:24.96 <Archive_home> Archive.setRawPosition! 50
@07:56:25.02 Archive.seek :home
@07:56:25.29 Archive.in :default
@07:56:25.37 <Lysis1_home> Lysis1.setRawPosition! 50
@07:56:25.43 Lysis1.seek :home
@07:56:25.51 <Air_home> Air.setRawPosition! 50
@07:56:25.55 Air.seek :home
@07:56:25.70 <Lysis1_home> Lysis1.in :default
@07:56:25.83 <Air_home> Air.in :default
@07:56:26.19 <LRAUV> Toroid.seek :clear
@07:56:27.81 Carousel.in :default
@07:56:27.87 Carousel.home.jog 0
@07:56:28.36 Carousel.in :default
@07:56:28.42 Carousel.seek 17
@07:56:28.59 Selecting Cartridge 20
@07:56:28.84 Carousel.seek 20
@07:56:29.72 Lysis1.in :fast
@07:56:29.79 Lysis1.seek :locked
@07:56:32.38 <Plunge> Plunge.seek :locked
@07:56:32.52 <Air> Air.in :fast
@07:56:32.58 Air.seek :locked
@07:56:35.18 <LRAUV> Powering cartridge bus
@07:56:37.30 Gate.setIR 1750,5000,0,6
@07:56:37.43 Gate.setIR 0,5000,0,6
@07:56:37.48 Gate.power :cartridge,:OFF
@07:56:38.02 Mounted archive Cartridge
@07:56:55.46 -> Cmd.startFiltering
@07:56:55.92 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@07:56:55.93 SamplePump.setPosition! 0ml
@07:56:55.97 Bypass.open
@07:56:57.42 Intake.open
@07:56:58.20 Sea 4.3m deep (21.1psia)
@07:56:58.22 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@07:57:00.79 Vacuum tare is 51% of full scale
@07:57:00.92 Pressure.in :default
@07:57:01.01 Pressure.hold
@07:57:01.20 SamplePump.in :prime
@07:57:01.27 SamplePump.seek 10ml,1:21
@07:57:10.65 Pressure.coast
@07:57:10.70 SamplePump.setPosition! 0ml
@07:57:10.75 Exhaust.open
@07:57:12.19 <closeBypass> Bypass.close
@07:57:13.70 <FILTERING> Pressure.in :default
@07:57:13.76 Pressure.hold
@07:57:13.88 Sea 4.2m deep (20.8psia)
@07:57:14.02 SamplePump.in :prime
@07:57:14.09 SamplePump.seek 100ml,8:51
@07:59:24.30 Pressure.coast
@07:59:24.36 Bypass.open
@07:59:25.06 Intake.close
@07:59:25.93 Toroid.in :hold
@07:59:25.99 Toroid.seek :crack
@07:59:27.54 Toroid.seek :cartridge
@07:59:28.63 Toroid.in :default
@07:59:28.69 Toroid.seek :clear
@07:59:30.65 Pressurizing cartridge to 26psi above 20.6psia
@07:59:30.66 SamplePump.setPosition! 0ml
@07:59:30.70 Intake.open
@07:59:31.44 Bypass.close
@07:59:32.16 Exhaust.close
@07:59:32.96 Pressure.in :default
@07:59:33.02 Pressure.hold
@07:59:33.19 SamplePump.in :charge
@07:59:33.25 SamplePump.seek 2.3ml,42 seconds
@07:59:39.59 Pressure.coast
@07:59:39.64 Intake.close
@07:59:40.47 Pressurized cartridge with 0.177ml to 26.4psi
@07:59:40.48 Isolated @41.0psia (20.4psi)
@07:59:40.49 Verifying cartridge holds at least 0.75..0.85 of 41.0psia
@07:59:40.93 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@07:59:45.56 Sampling 1000.0ml
@07:59:45.58 SamplePump.setPosition! 0ml
@07:59:45.64 Exhaust.open
@07:59:46.31 Intake.open
@07:59:47.17 Pressure.in :default
@07:59:47.23 Pressure.hold
@07:59:47.35 Sea 3.9m deep (20.5psia)
@07:59:47.48 SamplePump.in :sample
@07:59:47.55 SamplePump.seek 1000ml,1:23:53
@07:59:50.22 <sampling> Cartridge 20 filtering 1/1000ml at 0.27ml/s with 25.4psi vacuum
@08:00:50.21 Cartridge 20 filtering 19/1000ml at 0.34ml/s with 23.1psi vacuum 5.9m deep (23.4psia)
@08:01:50.21 Cartridge 20 filtering 37/1000ml at 0.33ml/s with 24.8psi vacuum 3.7m deep (20.1psia)
@08:02:50.21 Cartridge 20 filtering 54/1000ml at 0.24ml/s with 24.8psi vacuum 2.9m deep (18.9psia)
@08:03:50.21 Cartridge 20 filtering 72/1000ml at 0.29ml/s with 22.3psi vacuum 3.6m deep (20.0psia)
@08:04:50.21 Cartridge 20 filtering 88/1000ml at 0.30ml/s with 24.6psi vacuum 2.3m deep (18.1psia)
@08:05:50.21 Cartridge 20 filtering 105/1000ml at 0.29ml/s with 27.5psi vacuum 4.7m deep (21.7psia)
@08:06:50.21 Cartridge 20 filtering 121/1000ml at 0.28ml/s with 20.8psi vacuum 4.8m deep (21.8psia)
@08:07:50.21 Cartridge 20 filtering 137/1000ml at 0.29ml/s with 23.1psi vacuum 3.1m deep (19.3psia)
@08:08:50.21 Cartridge 20 filtering 153/1000ml at 0.28ml/s with 22.7psi vacuum 5.9m deep (23.5psia)
@08:09:50.21 Cartridge 20 filtering 168/1000ml at 0.27ml/s with 24.0psi vacuum
@08:10:50.21 Cartridge 20 filtering 184/1000ml at 0.20ml/s with 26.5psi vacuum 2.5m deep (18.3psia)
@08:11:50.21 Cartridge 20 filtering 199/1000ml at 0.27ml/s with 26.2psi vacuum 4.6m deep (21.5psia)
@08:12:50.21 Cartridge 20 filtering 214/1000ml at 0.26ml/s with 28.3psi vacuum
@08:13:50.21 Cartridge 20 filtering 228/1000ml at 0.22ml/s with 22.4psi vacuum 4.9m deep (21.9psia)
@08:14:50.21 Cartridge 20 filtering 243/1000ml at 0.20ml/s with 22.4psi vacuum 1.9m deep (17.5psia)
@08:15:50.21 Cartridge 20 filtering 257/1000ml at 0.25ml/s with 24.3psi vacuum 3.8m deep (20.4psia)
@08:16:50.21 Cartridge 20 filtering 272/1000ml at 0.20ml/s with 22.8psi vacuum 3.6m deep (20.1psia)
@08:17:50.21 Cartridge 20 filtering 286/1000ml at 0.25ml/s with 23.8psi vacuum 2.2m deep (18.0psia)
@08:18:50.21 Cartridge 20 filtering 299/1000ml at 0.20ml/s with 26.5psi vacuum 4.3m deep (21.0psia)
@08:19:50.21 Cartridge 20 filtering 313/1000ml at 0.22ml/s with 19.1psi vacuum 3.1m deep (19.3psia)
@08:20:50.21 Cartridge 20 filtering 327/1000ml at 0.21ml/s with 23.2psi vacuum 3.8m deep (20.3psia)
@08:21:50.21 Cartridge 20 filtering 340/1000ml at 0.24ml/s with 19.1psi vacuum 5.9m deep (23.4psia)
@08:22:50.21 Cartridge 20 filtering 354/1000ml at 0.25ml/s with 25.0psi vacuum 2.3m deep (18.2psia)
@08:23:50.21 Cartridge 20 filtering 367/1000ml at 0.20ml/s with 24.3psi vacuum 4.6m deep (21.5psia)
@08:24:50.21 Cartridge 20 filtering 380/1000ml at 0.20ml/s with 22.3psi vacuum
@08:25:50.21 Cartridge 20 filtering 393/1000ml at 0.20ml/s with 26.1psi vacuum 4.4m deep (21.2psia)
@08:26:50.21 Cartridge 20 filtering 406/1000ml at 0.22ml/s with 19.9psi vacuum 2.9m deep (19.0psia)
@08:27:50.21 Cartridge 20 filtering 419/1000ml at 0.24ml/s with 25.3psi vacuum 3.4m deep (19.8psia)
@08:28:50.21 Cartridge 20 filtering 432/1000ml at 0.21ml/s with 23.4psi vacuum 5.3m deep (22.5psia)
@08:29:50.21 Cartridge 20 filtering 444/1000ml at 0.20ml/s with 24.7psi vacuum 1.7m deep (17.3psia)
@08:30:50.21 Cartridge 20 filtering 457/1000ml at 0.20ml/s with 29.9psi vacuum 4.8m deep (21.8psia)
@08:31:50.21 Cartridge 20 filtering 469/1000ml at 0.21ml/s with 27.3psi vacuum 2.3m deep (18.0psia)
@08:32:50.21 Cartridge 20 filtering 481/1000ml at 0.20ml/s with 24.1psi vacuum 4.1m deep (20.8psia)
@08:33:50.21 Cartridge 20 filtering 494/1000ml at 0.20ml/s with 24.3psi vacuum 4.3m deep (21.0psia)
@08:34:38.29 <FILTERING> Flow averaged 0.24ml/s over 34:51
@08:34:38.32 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi
@08:34:38.89 Relieved excess vacuum in .6 seconds
@08:34:38.92 SamplePump.seek 1000ml,41:54
@08:34:50.21 <sampling> Cartridge 20 filtering 506/1000ml at 0.20ml/s with 25.6psi vacuum 2.1m deep (17.8psia)
@08:34:57.04 <FILTERING> Flow averaged 0.20ml/s over 19 seconds
@08:34:57.07 Waiting up to 16 seconds for vacuum to reduce from 29.2 to 18.0psi
@08:34:57.64 Relieved excess vacuum in .6 seconds
@08:34:57.67 SamplePump.seek 1000ml,41:36
@08:35:41.23 Flow averaged 0.20ml/s over 44 seconds
@08:35:41.26 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi
@08:35:41.83 Relieved excess vacuum in .6 seconds
@08:35:41.86 SamplePump.seek 1000ml,40:52
@08:35:50.21 <sampling> Cartridge 20 filtering 518/1000ml at 0.20ml/s with 23.5psi vacuum 3.2m deep (19.4psia)
@08:36:44.70 <FILTERING> Flow averaged 0.20ml/s over 1:03
@08:36:44.73 Waiting up to 11 seconds for vacuum to reduce from 27.0 to 18.0psi
@08:36:45.31 Relieved excess vacuum in .6 seconds
@08:36:45.33 SamplePump.seek 1000ml,39:48
@08:36:50.20 <sampling> Cartridge 20 filtering 530/1000ml at 0.20ml/s with 23.9psi vacuum 2.2m deep (18.0psia)
@08:37:27.38 <FILTERING> Flow averaged 0.20ml/s over 43 seconds
@08:37:27.41 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi
@08:37:27.99 Relieved excess vacuum in .6 seconds
@08:37:28.01 SamplePump.seek 1000ml,39:06
@08:37:41.71 Flow averaged 0.20ml/s over 14 seconds
@08:37:41.73 <closeIntake> Intake.close
@08:37:41.81 <closeExhaust> Exhaust.close
@08:37:41.96 <FILTERING> Toroid.in :hold
@08:37:42.03 Toroid.seek :bypass
@08:37:43.65 Toroid.in :default
@08:37:43.71 Toroid.seek :clear
@08:37:45.32 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@08:37:45.33 Pressure.coast
@08:37:45.52 Sampled  539.9ml
@08:37:47.43 <LRAUV> -> Cmd.startProcessing
@08:37:47.82 <PROCESSING> Plunge.seek :reagent
@08:37:48.65 Sample loop closed. Ready to Deliver Reagents
@08:37:48.66 Archive.seek :sealed
@08:37:52.93 Archive.seek :evac
@08:38:06.86 delay 1:00
@08:39:06.88 Archive.seek :clear
@08:39:24.61 RNAlater soak for 10:00
@08:49:24.63 Archive.seek :tVent
@08:49:27.21 delay 1 second
@08:49:28.23 Archive.seek :evac
@08:49:43.86 delay 10 seconds
@08:49:53.89 Sample preserved and evacuated
@08:49:55.06 <LRAUV> -> Cmd.stop
@08:49:55.26 Carousel.seek 20
@08:49:55.43 Plunge.seek :locked
@08:49:56.38 <Archive> Archive.in :unlocking
@08:49:56.51 Archive.seek :clear
@08:49:56.57 <Air> Air.in :unlocking
@08:49:56.71 Air.seek :clear
@08:49:56.77 <Lysis1> Lysis1.in :unlocking
@08:49:56.91 Lysis1.seek :clear
@08:50:00.57 <LRAUV> Archive.coast
@08:50:00.61 Air.coast
@08:50:00.66 Lysis1.coast
@08:50:00.71 Lysis2.coast
@08:50:00.76 Plunge.coast
@08:50:00.81 SamplePump.coast
@08:50:00.86 Toroid.coast
@08:50:00.91 Carousel.coast
@08:50:00.96 PinPort.coast
@08:50:01.56 Gate.power :motor,:OFF
@08:50:01.57 Gate.power :core,:OFF
@08:50:01.60 Gate.power :analytic1,:OFF
@08:50:01.62 Gate.power :analytic2,:OFF
@08:50:01.64 Gate.power :analytic3,:OFF
@08:50:01.67 Gate.power :main,:OFF
@08:50:01.69 Safely stopped and ready to power off
##-- file closed 2021-04-24T16:01:57.775Z

##-- file opened 2021-04-24T16:01:57.778Z
@09:01:57.61 -> showStatus
@09:01:59.63 -> Cmd.loadCartridge -9
@09:01:59.77 Gate.power :main,:ON,limit to 5000mA
@09:01:59.78 Gate.power :analytic1,:ON,limit to 5000mA
@09:01:59.80 Gate.power :analytic2,:ON,limit to 5000mA
@09:01:59.83 Gate.power :analytic3,:ON,limit to 5000mA
@09:01:59.96 Powering core bus
@09:02:02.24 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@09:02:02.38 <LRAUV> Archive.configure :default
@09:02:02.85 Air.configure :default
@09:02:02.95 Lysis1.configure :default
@09:02:03.05 Lysis2.configure :default
@09:02:03.17 Plunge.configure :default
@09:02:03.30 SamplePump.configure :default
@09:02:03.44 ExtraPump.configure :default
@09:02:03.55 Toroid.configure :default
@09:02:03.68 Carousel.configure :default
@09:02:03.81 Pressure.configure :default
@09:02:03.92 PinPort.configure :default
@09:02:03.99 All elf controllers running firmware version 4.741
@09:02:04.02 Gate.power :motor,:ON,limit to 5000mA
@09:02:04.29 <Lysis1_home> Lysis1.in :default
@09:02:04.35 <Archive_home> Archive.in :default
@09:02:04.47 <Air_home> Air.in :default
@09:02:04.67 <Lysis1_home> Lysis1.home.jog 0
@09:02:04.69 <Air_home> Air.home.jog 0
@09:02:04.72 <Archive_home> Archive.home.jog 0
@09:02:04.93 <Toroid_home> Toroid.in :default
@09:02:05.00 <Plunge_home> Plunge.in :default
@09:02:05.09 <PinPort_home> PinPort.in :default
@09:02:05.13 <Lysis2_home> Lysis2.in :default
@09:02:05.30 <Plunge_home> Plunge.home.jog 0
@09:02:05.32 <PinPort_home> PinPort.home.jog 0
@09:02:05.35 <Toroid_home> Toroid.home.jog 0
@09:02:05.37 <Lysis2_home> Lysis2.home.jog 0
@09:02:05.69 <Air_home> Air.in :homing
@09:02:05.74 <Lysis1_home> Lysis1.in :homing
@09:02:05.80 <Archive_home> Archive.in :homing
@09:02:05.93 <Air_home> Air.jog 161
@09:02:05.97 <Archive_home> Archive.jog 154
@09:02:06.00 <Lysis1_home> Lysis1.jog 1417
@09:02:06.21 <Toroid_home> Toroid.in :homing
@09:02:06.23 <Plunge_home> Plunge.in :homing
@09:02:06.26 <PinPort_home> PinPort.in :homing
@09:02:06.30 <Lysis2_home> Lysis2.in :homing
@09:02:06.46 <Plunge_home> Plunge.jog 1577
@09:02:06.49 <PinPort_home> PinPort.jog -107
@09:02:06.52 <Toroid_home> Toroid.jog 618
@09:02:06.54 <Lysis2_home> Lysis2.jog 1428
@09:02:06.72 <Air_home> Air.setRawPosition! 50
@09:02:06.74 <Archive_home> Archive.setRawPosition! 50
@09:02:06.81 <Air_home> Air.seek :home
@09:02:06.85 <Archive_home> Archive.seek :home
@09:02:06.96 <PinPort_home> PinPort.setRawPosition! 0
@09:02:07.01 PinPort.seek :home
@09:02:07.09 PinPort.in :default
@09:02:07.20 <Air_home> Air.in :default
@09:02:07.23 <Archive_home> Archive.in :default
@09:02:07.35 <Lysis1_home> Lysis1.setRawPosition! 50
@09:02:07.41 Lysis1.seek :home
@09:02:07.68 Lysis1.in :default
@09:02:07.79 <Plunge_home> Plunge.setRawPosition! 50
@09:02:07.81 <Lysis2_home> Lysis2.setRawPosition! 50
@09:02:07.88 <Plunge_home> Plunge.seek :home
@09:02:07.92 <Lysis2_home> Lysis2.seek :home
@09:02:08.23 Lysis2.in :default
@09:02:08.32 <Plunge_home> Plunge.in :default
@09:02:08.73 <Toroid_home> Toroid.setRawPosition! 0
@09:02:08.78 Toroid.seek :home
@09:02:08.86 Toroid.in :default
@09:02:08.99 <LRAUV> Toroid.seek :clear
@09:02:10.61 Carousel.in :default
@09:02:10.68 Carousel.home.jog 0
@09:02:11.16 Carousel.in :default
@09:02:11.22 Carousel.seek 20
@09:02:11.39 Selecting Cartridge 15
@09:02:11.63 Carousel.seek 15
@09:02:12.93 Lysis1.in :fast
@09:02:12.99 Lysis1.seek :locked
@09:02:15.59 <Plunge> Plunge.seek :locked
@09:02:15.73 <Air> Air.in :fast
@09:02:15.80 Air.seek :locked
@09:02:18.41 <LRAUV> Powering cartridge bus
@09:02:20.53 Gate.setIR 1750,5000,0,6
@09:02:20.66 Gate.setIR 0,5000,0,6
@09:02:20.72 PuckHeater.configure :default
@09:02:20.76 Gate.power :heat,:ON,limit to 5000mA
@09:02:21.44 Mounted lysis Cartridge board#Q05031
@09:02:21.47 Using default puck heat loss curve for HeatingAccelInCarousel
@09:02:40.03 -> Cmd.startFiltering
@09:02:40.49 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@09:02:40.50 SamplePump.setPosition! 0ml
@09:02:40.55 Bypass.open
@09:02:42.00 Intake.open
@09:02:42.81 Sea 7.3m deep (25.5psia)
@09:02:42.83 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@09:02:45.41 Vacuum tare is 51% of full scale
@09:02:45.54 Pressure.in :default
@09:02:45.60 Pressure.hold
@09:02:45.77 SamplePump.in :prime
@09:02:45.83 SamplePump.seek 10ml,1:21
@09:02:55.22 Pressure.coast
@09:02:55.27 SamplePump.setPosition! 0ml
@09:02:55.32 Exhaust.open
@09:02:56.74 <closeBypass> Bypass.close
@09:02:58.27 <FILTERING> Pressure.in :default
@09:02:58.33 Pressure.hold
@09:02:58.45 Sea 7.0m deep (25.1psia)
@09:02:58.58 SamplePump.in :prime
@09:02:58.65 SamplePump.seek 100ml,8:51
@09:05:08.96 Pressure.coast
@09:05:09.02 Bypass.open
@09:05:09.73 Intake.close
@09:05:10.60 Toroid.in :hold
@09:05:10.66 Toroid.seek :crack
@09:05:12.21 Toroid.seek :cartridge
@09:05:13.31 Toroid.in :default
@09:05:13.37 Toroid.seek :clear
@09:05:15.32 Pressurizing cartridge to 26psi above 25.6psia
@09:05:15.33 SamplePump.setPosition! 0ml
@09:05:15.38 Intake.open
@09:05:16.12 Bypass.close
@09:05:16.88 Exhaust.close
@09:05:17.67 Pressure.in :default
@09:05:17.73 Pressure.hold
@09:05:17.90 SamplePump.in :charge
@09:05:17.97 SamplePump.seek 2.3ml,42 seconds
@09:05:27.18 Pressure.coast
@09:05:27.23 Intake.close
@09:05:28.06 Pressurized cartridge with 0.345ml to 26.3psi
@09:05:28.08 Isolated @45.6psia (20.1psi)
@09:05:28.09 Verifying cartridge holds at least 0.75..0.85 of 45.6psia
@09:05:28.13 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@09:05:32.75 Sampling 1000.0ml
@09:05:32.77 SamplePump.setPosition! 0ml
@09:05:32.82 Exhaust.open
@09:05:33.50 Intake.open
@09:05:34.38 Pressure.in :default
@09:05:34.44 Pressure.hold
@09:05:34.56 Sea 7.3m deep (25.6psia)
@09:05:34.69 SamplePump.in :sample
@09:05:34.76 SamplePump.seek 1000ml,1:23:53
@09:05:37.42 <sampling> Cartridge 15 filtering 1/1000ml at 0.37ml/s with 23.6psi vacuum 3.9m deep (20.4psia)
@09:06:37.41 Cartridge 15 filtering 19/1000ml at 0.33ml/s with 24.3psi vacuum 2.3m deep (18.1psia)
@09:07:37.42 Cartridge 15 filtering 37/1000ml at 0.31ml/s with 27.1psi vacuum 4.6m deep (21.5psia)
@09:08:37.41 Cartridge 15 filtering 55/1000ml at 0.28ml/s with 23.0psi vacuum 1.8m deep (17.3psia)
@09:09:37.42 Cartridge 15 filtering 72/1000ml at 0.26ml/s with 21.9psi vacuum 4.1m deep (20.8psia)
@09:10:37.41 Cartridge 15 filtering 88/1000ml at 0.27ml/s with 26.4psi vacuum 3.6m deep (20.0psia)
@09:11:37.42 Cartridge 15 filtering 105/1000ml at 0.24ml/s with 23.2psi vacuum 1.8m deep (17.4psia)
@09:12:37.41 Cartridge 15 filtering 120/1000ml at 0.26ml/s with 21.8psi vacuum 5.8m deep (23.3psia)
@09:13:37.42 Cartridge 15 filtering 136/1000ml at 0.29ml/s with 23.6psi vacuum 3.1m deep (19.2psia)
@09:14:37.41 Cartridge 15 filtering 151/1000ml at 0.25ml/s with 28.5psi vacuum 3.2m deep (19.4psia)
@09:15:37.42 Cartridge 15 filtering 166/1000ml at 0.25ml/s with 22.7psi vacuum 2.7m deep (18.7psia)
@09:16:37.41 Cartridge 15 filtering 181/1000ml at 0.27ml/s with 25.0psi vacuum 3.3m deep (19.5psia)
@09:17:37.42 Cartridge 15 filtering 196/1000ml at 0.25ml/s with 21.9psi vacuum 4.4m deep (21.2psia)
@09:18:37.41 Cartridge 15 filtering 210/1000ml at 0.23ml/s with 22.7psi vacuum
@09:19:37.42 Cartridge 15 filtering 224/1000ml at 0.24ml/s with 22.0psi vacuum 5.2m deep (22.4psia)
@09:20:37.41 Cartridge 15 filtering 238/1000ml at 0.22ml/s with 28.2psi vacuum 1.9m deep (17.5psia)
@09:21:37.42 Cartridge 15 filtering 251/1000ml at 0.24ml/s with 21.8psi vacuum 3.6m deep (20.1psia)
@09:22:37.41 Cartridge 15 filtering 265/1000ml at 0.25ml/s with 24.3psi vacuum 5.1m deep (22.3psia)
@09:23:37.42 Cartridge 15 filtering 278/1000ml at 0.20ml/s with 24.3psi vacuum 1.9m deep (17.5psia)
@09:24:37.41 Cartridge 15 filtering 291/1000ml at 0.20ml/s with 24.5psi vacuum 4.6m deep (21.5psia)
@09:25:37.42 Cartridge 15 filtering 304/1000ml at 0.23ml/s with 22.6psi vacuum 3.4m deep (19.7psia)
@09:26:37.41 Cartridge 15 filtering 317/1000ml at 0.23ml/s with 22.8psi vacuum 3.6m deep (20.1psia)
@09:27:37.42 Cartridge 15 filtering 329/1000ml at 0.20ml/s with 24.3psi vacuum 5.0m deep (22.1psia)
@09:28:37.41 Cartridge 15 filtering 342/1000ml at 0.20ml/s with 24.7psi vacuum 2.1m deep (17.8psia)
@09:29:37.42 Cartridge 15 filtering 354/1000ml at 0.21ml/s with 25.3psi vacuum 6.1m deep (23.6psia)
@09:30:37.41 Cartridge 15 filtering 366/1000ml at 0.20ml/s with 26.2psi vacuum 2.5m deep (18.4psia)
@09:31:37.42 Cartridge 15 filtering 378/1000ml at 0.20ml/s with 26.2psi vacuum 2.6m deep (18.6psia)
@09:32:37.41 Cartridge 15 filtering 391/1000ml at 0.20ml/s with 27.2psi vacuum 3.9m deep (20.4psia)
@09:32:41.91 <FILTERING> Flow averaged 0.24ml/s over 27:08
@09:32:41.94 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@09:32:42.76 Relieved excess vacuum in .9 seconds
@09:32:42.79 SamplePump.seek 1000ml,51:15
@09:33:11.48 Flow averaged 0.20ml/s over 29 seconds
@09:33:11.51 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi
@09:33:12.33 Relieved excess vacuum in .9 seconds
@09:33:12.36 SamplePump.seek 1000ml,50:46
@09:33:15.41 Flow estimate reduced to 0.16ml/s
@09:33:15.44 <closeIntake> Intake.close
@09:33:15.49 <closeExhaust> Exhaust.close
@09:33:15.66 <FILTERING> Toroid.in :hold
@09:33:15.73 Toroid.seek :bypass
@09:33:17.35 Toroid.in :default
@09:33:17.41 Toroid.seek :clear
@09:33:19.02 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@09:33:19.03 Pressure.coast
@09:33:19.22 Sampled  397.9ml
@09:33:21.15 <LRAUV> -> Cmd.startProcessing
@09:33:22.59 <PROCESSING> SPR.command \"read_sequences\"
@09:33:22.63 SPR.command \"stats_on\"
@09:33:23.40 SPR.command \"start 0 1\"
@09:33:25.41 <SPRlogger> Begun log /var/log/esp/spr21apr2409h.spr
@09:33:26.25 <PROCESSING> Handoff.select :waste
@09:33:32.94 Syringe tare is 20% of full scale
@09:33:32.96 PinPort.in :locking
@09:33:33.03 PinPort.seek :locked
@09:33:33.51 <posArchive> Archive.in :fast
@09:33:33.56 <posLyS2> Lysis2.in :fast
@09:33:33.60 <PROCESSING> Lysis1.seek 1350uL
@09:33:33.72 <posLyS2> Lysis2.seek :full
@09:33:33.79 <posArchive> Archive.seek :full
@09:33:35.84 <PROCESSING> Plunge.seek :reagent
@09:33:36.74 Evacuating puck with filter bubble point of 50psi
@09:33:36.76 PinPort.seek :locked
@09:33:36.84 Handoff.select :waste
@09:33:43.43 Air syringe pumping up to 1 stroke to reach 8psi
@09:33:43.44 Air stroke #1 of 1
@09:33:43.53 Air.in :pressurize
@09:33:43.59 Air.seek :pressurized
@09:33:54.84 -0.0psi after pressurize
@09:33:54.85 Air.in :compress
@09:33:54.92 Air.seek :evac
@09:33:59.01 0.5psi after compress
@09:34:11.58 0.3psi after compress (stable)
@09:34:11.60 Air.in :default
@09:34:11.66 Air.hold
@09:34:11.83 at 0.2psi Opened to waste
@09:34:11.85 Handoff.select :blocked
@09:34:11.98 Archive syringe pumping up to 5 strokes to reach 50psi
@09:34:11.99 Archive stroke #1 of 5
@09:34:12.07 Archive.in :pressurize
@09:34:12.15 Archive.seek :pressurized
@09:34:24.89 11.3psi after pressurize
@09:34:24.90 Archive.in :compress
@09:34:24.97 Archive.seek :empty
@09:34:40.86 30.6psi after compress
@09:34:58.44 37.6psi after compress (stable)
@09:34:58.46 Archive.in :gulp
@09:34:58.54 Archive.seek :topVent
@09:35:05.75 37.8psi after gulp
@09:35:18.35 37.6psi after gulp (stable)
@09:35:18.36 Archive stroke #2 of 5
@09:35:18.44 Archive.in :pressurize
@09:35:18.50 Archive.seek :pressurized
@09:35:31.25 37.4psi after pressurize
@09:35:31.26 Archive.in :compress
@09:35:31.35 Archive.seek :empty
@09:35:47.32 Reached 50.2 of 50.0psi after pumping 3460uL (2.00 strokes)
@09:35:47.33 Archive.in :default
@09:35:47.40 Archive.hold
@09:35:47.47 Handoff.select :waste
@09:35:56.66 delay 1:00
@09:36:56.68 Handoff.select :blocked
@09:37:19.38 Handoff.select :waste
@09:37:19.51 0.5psi at evacEnd
@09:37:19.52 Handoff.select :blocked
@09:37:19.65 Archive.in :fast
@09:37:19.72 Archive.seek :topVent
@09:37:33.43 Stable @0.9psi after Handoff blocked
@09:37:33.45 Archive syringe pumping up to 5 strokes to reach 31psi
@09:37:33.47 Archive stroke #1 of 5
@09:37:33.57 Archive.in :pressurize
@09:37:33.64 Archive.seek :pressurized
@09:37:46.38 1.2psi after pressurize
@09:37:46.39 Archive.in :compress
@09:37:46.46 Archive.seek :empty
@09:38:02.35 6.3psi after compress
@09:38:37.50 Reached 30.9 of 30.9psi after pumping 1730uL (1.00 strokes)
@09:38:37.51 Archive.in :default
@09:38:37.58 Archive.hold
@09:38:37.64 Handoff.select :waste
@09:38:53.83 delay 1:00
@09:39:53.85 Handoff.select :blocked
@09:40:19.53 Handoff.select :waste
@09:40:19.65 3.7psi at evacEnd
@09:40:19.67 Air.seek :fullVent
@09:40:21.11 Archive.in :fast
@09:40:21.17 Archive.seek :topVent
@09:40:28.32 Seawater evacuated, Presure above/below filter released. Lysis begins
@09:40:28.41 Lysis1.in :slow
@09:40:28.47 Lysis1.seek 1050uL
@09:40:58.49 delay 5 seconds
@09:41:03.51 Handoff.select :blocked
@09:41:03.70 Lysis1.seek 450uL
@09:42:03.66 delay 3 seconds
@09:42:06.73 Air.in to mix into 1.37806 parts Lysis1
@09:42:07.22 <Airmixer> Air.seek :evac
@09:42:07.26 <PROCESSING> Lysis1.seek 300uL
@09:42:22.37 Lysis1.seek :empty
@09:42:52.46 Lysis buffer delivered @14.3psi
@09:42:52.47 Polling Can every 2:00
@09:42:53.02 PuckHeater.configure :default #ambient 11.4C
@09:42:53.07 PuckHeater.seek 80.2C
@09:43:50.03 PuckHeater.hold 2:00,80.2C
@09:45:49.99 Polling Can every 2:00
@09:45:50.54 PuckHeater.configure :default #ambient 11.5C
@09:45:50.59 PuckHeater.hold 30 seconds,79.2C
@09:46:20.74 Polling Can every 2:00
@09:46:21.28 PuckHeater.configure :default #ambient 11.5C
@09:46:21.34 PuckHeater.hold 30 seconds,78.7C
@09:46:51.51 Polling Can every 2:00
@09:46:52.06 PuckHeater.configure :default #ambient 11.5C
@09:46:52.11 PuckHeater.hold 10:00,78.1C
@09:56:51.41 Lysis complete. Syringe pressure @17.1psi
@09:56:51.42 Handoff.select :blocked
@09:56:51.46 Archive syringe pumping up to 3 strokes to reach 40psi
@09:56:51.47 Archive stroke #1 of 3
@09:56:51.56 Archive.in :pressurize
@09:56:51.63 Archive.seek :pressurized
@09:57:04.36 16.9psi after pressurize
@09:57:04.37 Archive.in :compress
@09:57:04.45 Archive.seek :empty
@09:57:15.85 Reached 40.2 of 40.0psi after pumping 1634uL (0.94 strokes)
@09:57:15.88 Archive.in :default
@09:57:15.95 Archive.hold
@09:57:16.02 delay 2 seconds
@09:57:18.04 Archive.in :fast
@09:57:18.10 Archive.seek :full
@09:57:24.98 Lysis1.seek 138uL
@09:57:38.86 delay 1 second
@09:57:39.89 <fillArchive> Archive.seek :full
@09:57:39.91 <PROCESSING> Lysis1.seek :empty
@09:57:53.88 Initial air pressure @53.5psi
@09:57:53.95 Lysis1.in :extract
@09:57:54.02 Lysis1.seek 600uL,nil
@09:57:56.37 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@09:57:56.38 Archive stroke #1 of 6
@09:57:56.53 Archive.in :pressurize
@09:57:56.60 Archive.seek :pressurized
@09:58:09.34 15.9psi after pressurize
@09:58:09.35 Archive.in :compress
@09:58:09.43 Archive.seek :empty
@09:58:25.34 17.0psi after compress
@09:58:25.89 Archive.in :default
@09:58:25.95 Archive.hold
@09:58:26.10 <PROCESSING> Initial 600uL lysate @16.7psi
@09:58:26.11 Handoff.select :blocked
@09:58:26.20 Initial air pressure @17.7psi
@09:58:26.27 Lysis1.seek 825uL,nil
@09:58:27.47 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@09:58:27.48 Archive stroke #1 of 6
@09:58:27.56 Archive.in :compress
@09:58:27.63 Archive.seek :empty
@09:58:27.82 15.8psi after compress
@09:58:28.15 Reached 17.4 of 17.0psi after pumping 1730uL (1.00 strokes)
@09:58:28.16 Archive.in :default
@09:58:28.24 Archive.hold
@09:58:28.96 Archive syringe pumping up to 6 strokes to reach 17psi
@09:58:28.97 Archive stroke #1 of 6
@09:58:29.07 Archive.in :compress
@09:58:29.14 Archive.seek :empty
@09:58:29.32 15.2psi after compress
@09:58:29.76 Reached 17.2 of 17.0psi after pumping 1730uL (1.00 strokes)
@09:58:29.77 Archive.in :default
@09:58:29.84 Archive.hold
@09:58:30.61 Archive syringe pumping up to 6 strokes to reach 17psi
@09:58:30.62 Archive stroke #1 of 6
@09:58:30.75 Archive.in :compress
@09:58:30.82 Archive.seek :empty
@09:58:30.99 15.5psi after compress
@09:58:31.42 Reached 17.0 of 17.0psi after pumping 1730uL (1.00 strokes)
@09:58:31.44 Archive.in :default
@09:58:31.50 Archive.hold
@09:58:32.26 Archive syringe pumping up to 6 strokes to reach 17psi
@09:58:32.27 Archive stroke #1 of 6
@09:58:32.36 Archive.in :compress
@09:58:32.42 Archive.seek :empty
@09:58:32.70 15.9psi after compress
@09:58:45.28 15.9psi after compress (stable)
@09:58:48.02 Archive.in :default
@09:58:48.06 Archive.hold
@09:58:48.24 <PROCESSING> Recovered 825uL lysate @15.5psi
@09:58:48.26 Air.in :halfSpeed
@09:58:48.33 Air.seek :fullVent
@09:58:50.57 Puck Top Vented, bottom @4.2psi
@09:58:50.58 Handoff.select :waste
@09:58:50.70 delay 5 seconds
@09:58:55.76 Handoff open to waste, bottom @0.2psi
@09:58:55.78 Air.in :default
@09:58:55.84 Air.seek :evac
@09:58:57.28 Lysis2.in :DAmix
@09:58:57.38 Lysis1.in :DAmix
@09:58:57.82 <Lysis2mixer> Lysis2.seek 1480uL
@09:58:57.87 <PROCESSING> Lysis1.seek 575uL
@09:59:32.92 delay 5 seconds
@09:59:37.94 Handoff.select SPR
@09:59:38.05 SPR.command \"start 2 14\"
@09:59:39.27 delay 10 seconds
@09:59:49.29 SPR.command \"start 1 0\"
@09:59:52.52 Waiting up to 4:00 for SPR to enter state 1
@10:01:54.90 SPR.command \"hold_on\"
@10:01:56.32 SPR Dilution 1 of 1 -- Running Experiment 1
@10:01:56.75 <Lysis2mixer> Lysis2.seek 1180uL
@10:01:56.79 <PROCESSING> Lysis1.seek 275uL
@10:02:38.82 volL1 = 300.0 and volL2 = 300.0
@10:02:38.83 delay 15 seconds
@10:02:53.85 SPR.command \"hold_off\"
@10:02:56.97 Waiting up to 38:00 for SPR to enter state 7
@10:29:40.51 SPR Analysis has Completed
@10:29:40.53 Handoff.select :waste
@10:29:41.44 <SPRlogger> Ended log /var/log/esp/spr21apr2409h.spr
@10:29:41.45 <PROCESSING> SPR.command \"stats_off\"
@10:29:42.82 <LRAUV> -> Cmd.stop
@10:29:42.91 <closeHandoff> Handoff.select :close
@10:29:43.03 <LRAUV> Gate.power :heat,:OFF
@10:29:43.05 Gate.power :cartridge,:OFF
@10:29:43.13 Carousel.seek 15
@10:29:43.30 Plunge.seek :locked
@10:29:44.19 PinPort.in :unlocking
@10:29:44.25 PinPort.seek :clear
@10:29:44.79 <Archive> Archive.in :unlocking
@10:29:44.92 Archive.seek :clear
@10:29:44.99 <Air> Air.in :unlocking
@10:29:45.11 Air.seek :clear
@10:29:45.15 <Lysis1> Lysis1.in :unlocking
@10:29:45.31 Lysis1.seek :clear
@10:29:45.34 <Lysis2> Lysis2.in :unlocking
@10:29:45.45 Lysis2.seek :clear
@10:29:48.96 <LRAUV> Archive.coast
@10:29:49.00 Air.coast
@10:29:49.05 Lysis1.coast
@10:29:49.10 Lysis2.coast
@10:29:49.15 Plunge.coast
@10:29:49.20 SamplePump.coast
@10:29:49.24 Toroid.coast
@10:29:49.29 Carousel.coast
@10:29:49.34 PinPort.coast
@10:29:49.52 Gate.power :motor,:OFF
@10:29:49.54 Gate.power :core,:OFF
@10:29:49.56 Gate.power :analytic1,:OFF
@10:29:49.58 Gate.power :analytic2,:OFF
@10:29:49.60 Gate.power :analytic3,:OFF
@10:29:49.62 Gate.power :main,:OFF
@10:29:49.64 Safely stopped and ready to power off
##-- file closed 2021-04-24T19:36:24.634Z

##-- file opened 2021-04-24T19:36:24.636Z
@12:36:24.84 -> showStatus
@12:36:26.86 -> Cmd.loadCartridge -6
@12:36:27.00 Gate.power :main,:ON,limit to 5000mA
@12:36:27.01 Gate.power :analytic1,:ON,limit to 5000mA
@12:36:27.03 Gate.power :analytic2,:ON,limit to 5000mA
@12:36:27.06 Gate.power :analytic3,:ON,limit to 5000mA
@12:36:27.19 Powering core bus
@12:36:29.47 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@12:36:29.95 <LRAUV> Archive.configure :default
@12:36:30.07 Air.configure :default
@12:36:30.17 Lysis1.configure :default
@12:36:30.28 Lysis2.configure :default
@12:36:30.40 Plunge.configure :default
@12:36:30.53 SamplePump.configure :default
@12:36:30.67 ExtraPump.configure :default
@12:36:30.77 Toroid.configure :default
@12:36:30.90 Carousel.configure :default
@12:36:31.03 Pressure.configure :default
@12:36:31.15 PinPort.configure :default
@12:36:31.21 All elf controllers running firmware version 4.741
@12:36:31.24 Gate.power :motor,:ON,limit to 5000mA
@12:36:31.40 <Archive_home> Archive.in :default
@12:36:31.70 Archive.home.jog 0
@12:36:31.86 <Toroid_home> Toroid.in :default
@12:36:31.94 <Plunge_home> Plunge.in :default
@12:36:31.98 <Lysis1_home> Lysis1.in :default
@12:36:32.02 <PinPort_home> PinPort.in :default
@12:36:32.05 <Air_home> Air.in :default
@12:36:32.11 <Lysis2_home> Lysis2.in :default
@12:36:32.29 <Toroid_home> Toroid.home.jog 0
@12:36:32.34 <Plunge_home> Plunge.home.jog 0
@12:36:32.40 <Lysis1_home> Lysis1.home.jog 0
@12:36:32.44 <Air_home> Air.home.jog 0
@12:36:32.47 <PinPort_home> PinPort.home.jog 0
@12:36:32.51 <Lysis2_home> Lysis2.home.jog 0
@12:36:32.89 <Archive_home> Archive.in :homing
@12:36:33.05 <Toroid_home> Toroid.in :homing
@12:36:33.08 <Plunge_home> Plunge.in :homing
@12:36:33.13 <Archive_home> Archive.jog 151
@12:36:33.19 <Lysis1_home> Lysis1.in :homing
@12:36:33.21 <PinPort_home> PinPort.in :homing
@12:36:33.38 <Toroid_home> Toroid.jog 622
@12:36:33.44 <Plunge_home> Plunge.jog 1573
@12:36:33.47 <PinPort_home> PinPort.jog -107
@12:36:33.51 <Lysis1_home> Lysis1.jog 1418
@12:36:33.70 <Air_home> Air.in :homing
@12:36:33.72 <Lysis2_home> Lysis2.in :homing
@12:36:33.80 <Air_home> Air.jog 173
@12:36:33.89 <Lysis2_home> Lysis2.jog 151
@12:36:33.98 <Archive_home> Archive.setRawPosition! 50
@12:36:34.00 <PinPort_home> PinPort.setRawPosition! 0
@12:36:34.07 <Archive_home> Archive.seek :home
@12:36:34.14 <PinPort_home> PinPort.seek :home
@12:36:34.24 PinPort.in :default
@12:36:34.34 <Air_home> Air.setRawPosition! 50
@12:36:34.40 Air.seek :home
@12:36:34.48 <Lysis2_home> Lysis2.setRawPosition! 50
@12:36:34.50 <Archive_home> Archive.in :default
@12:36:34.57 <Lysis2_home> Lysis2.seek :home
@12:36:34.68 <Air_home> Air.in :default
@12:36:34.79 <Plunge_home> Plunge.setRawPosition! 50
@12:36:34.81 <Lysis1_home> Lysis1.setRawPosition! 50
@12:36:34.90 <Plunge_home> Plunge.seek :home
@12:36:34.93 <Lysis1_home> Lysis1.seek :home
@12:36:35.05 <Lysis2_home> Lysis2.in :default
@12:36:35.25 <Plunge_home> Plunge.in :default
@12:36:35.32 <Lysis1_home> Lysis1.in :default
@12:36:35.60 <Toroid_home> Toroid.setRawPosition! 0
@12:36:35.65 Toroid.seek :home
@12:36:35.74 Toroid.in :default
@12:36:35.86 <LRAUV> Toroid.seek :clear
@12:36:37.49 Carousel.in :default
@12:36:37.55 Carousel.home.jog 0
@12:36:38.04 Carousel.in :default
@12:36:38.10 Carousel.seek 15
@12:36:38.23 Selecting Cartridge 18
@12:36:38.48 Carousel.seek 18
@12:36:39.36 Lysis1.in :fast
@12:36:39.42 Lysis1.seek :locked
@12:36:42.02 <Plunge> Plunge.seek :locked
@12:36:42.16 <Air> Air.in :fast
@12:36:42.22 Air.seek :locked
@12:36:44.83 <LRAUV> Powering cartridge bus
@12:36:46.95 Gate.setIR 1750,5000,0,6
@12:36:47.08 Gate.setIR 0,5000,0,6
@12:36:47.13 Gate.power :cartridge,:OFF
@12:36:47.66 Mounted archive Cartridge
@12:37:06.46 -> Cmd.startFiltering
@12:37:06.92 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@12:37:06.93 SamplePump.setPosition! 0ml
@12:37:06.98 Bypass.open
@12:37:08.49 Intake.open
@12:37:09.27 Sea 7.1m deep (25.3psia)
@12:37:09.29 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@12:37:11.86 Vacuum tare is 51% of full scale
@12:37:11.99 Pressure.in :default
@12:37:12.05 Pressure.hold
@12:37:12.22 SamplePump.in :prime
@12:37:12.31 SamplePump.seek 10ml,1:21
@12:37:21.74 Pressure.coast
@12:37:21.79 SamplePump.setPosition! 0ml
@12:37:21.84 Exhaust.open
@12:37:23.25 <closeBypass> Bypass.close
@12:37:24.82 <FILTERING> Pressure.in :default
@12:37:24.88 Pressure.hold
@12:37:24.99 Sea 6.5m deep (24.4psia)
@12:37:25.12 SamplePump.in :prime
@12:37:25.19 SamplePump.seek 100ml,8:51
@12:39:35.40 Pressure.coast
@12:39:35.46 Bypass.open
@12:39:36.19 Intake.close
@12:39:37.01 Toroid.in :hold
@12:39:37.08 Toroid.seek :crack
@12:39:38.64 Toroid.seek :cartridge
@12:39:39.73 Toroid.in :default
@12:39:39.80 Toroid.seek :clear
@12:39:41.76 Pressurizing cartridge to 26psi above 24.3psia
@12:39:41.77 SamplePump.setPosition! 0ml
@12:39:41.82 Intake.open
@12:39:42.55 Bypass.close
@12:39:43.25 Exhaust.close
@12:39:44.01 Pressure.in :default
@12:39:44.07 Pressure.hold
@12:39:44.24 SamplePump.in :charge
@12:39:44.31 SamplePump.seek 2.3ml,42 seconds
@12:39:50.67 Pressure.coast
@12:39:50.73 Intake.close
@12:39:51.57 Pressurized cartridge with 0.157ml to 26.5psi
@12:39:51.58 Isolated @43.0psia (18.7psi)
@12:39:51.59 Verifying cartridge holds at least 0.75..0.85 of 43.0psia
@12:39:51.62 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@12:39:56.25 Sampling 1000.0ml
@12:39:56.26 SamplePump.setPosition! 0ml
@12:39:56.31 Exhaust.open
@12:39:56.98 Intake.open
@12:39:57.86 Pressure.in :default
@12:39:57.92 Pressure.hold
@12:39:58.04 Sea 5.7m deep (23.1psia)
@12:39:58.17 SamplePump.in :sample
@12:39:58.24 SamplePump.seek 1000ml,1:23:53
@12:40:00.91 <sampling> Cartridge 18 filtering 1/1000ml at 0.28ml/s with 26.7psi vacuum
@12:41:00.90 Cartridge 18 filtering 20/1000ml at 0.35ml/s with 21.1psi vacuum 3.9m deep (20.5psia)
@12:42:00.90 Cartridge 18 filtering 38/1000ml at 0.34ml/s with 22.8psi vacuum 4.6m deep (21.4psia)
@12:43:00.90 Cartridge 18 filtering 56/1000ml at 0.30ml/s with 21.6psi vacuum 2.1m deep (17.8psia)
@12:44:00.90 Cartridge 18 filtering 74/1000ml at 0.29ml/s with 28.3psi vacuum 4.9m deep (22.0psia)
@12:45:00.90 Cartridge 18 filtering 92/1000ml at 0.31ml/s with 22.9psi vacuum 3.2m deep (19.5psia)
@12:46:00.90 Cartridge 18 filtering 109/1000ml at 0.30ml/s with 28.2psi vacuum 2.8m deep (18.9psia)
@12:47:00.90 Cartridge 18 filtering 126/1000ml at 0.30ml/s with 25.6psi vacuum 2.7m deep (18.7psia)
@12:48:00.90 Cartridge 18 filtering 142/1000ml at 0.24ml/s with 21.2psi vacuum 2.2m deep (18.0psia)
@12:49:00.90 Cartridge 18 filtering 159/1000ml at 0.23ml/s with 22.3psi vacuum 4.2m deep (20.9psia)
@12:50:00.90 Cartridge 18 filtering 175/1000ml at 0.24ml/s with 21.1psi vacuum
@12:51:00.90 Cartridge 18 filtering 191/1000ml at 0.28ml/s with 24.2psi vacuum 5.4m deep (22.7psia)
@12:52:00.90 Cartridge 18 filtering 206/1000ml at 0.26ml/s with 26.7psi vacuum 2.5m deep (18.4psia)
@12:53:00.90 Cartridge 18 filtering 222/1000ml at 0.27ml/s with 24.6psi vacuum 5.4m deep (22.7psia)
@12:54:00.90 Cartridge 18 filtering 237/1000ml at 0.27ml/s with 23.8psi vacuum 2.2m deep (17.9psia)
@12:55:00.90 Cartridge 18 filtering 252/1000ml at 0.27ml/s with 24.0psi vacuum 4.1m deep (20.8psia)
@12:56:00.90 Cartridge 18 filtering 267/1000ml at 0.21ml/s with 28.1psi vacuum 2.8m deep (18.8psia)
@12:57:00.90 Cartridge 18 filtering 281/1000ml at 0.26ml/s with 28.0psi vacuum 2.0m deep (17.7psia)
@12:58:00.90 Cartridge 18 filtering 296/1000ml at 0.21ml/s with 27.0psi vacuum 4.9m deep (22.0psia)
@12:59:00.90 Cartridge 18 filtering 310/1000ml at 0.26ml/s with 19.8psi vacuum 3.7m deep (20.2psia)
@13:00:00.90 Cartridge 18 filtering 324/1000ml at 0.24ml/s with 20.2psi vacuum 5.1m deep (22.2psia)
@13:01:00.90 Cartridge 18 filtering 337/1000ml at 0.20ml/s with 26.4psi vacuum
@13:02:00.90 Cartridge 18 filtering 351/1000ml at 0.23ml/s with 27.7psi vacuum 3.1m deep (19.3psia)
@13:03:00.90 Cartridge 18 filtering 365/1000ml at 0.21ml/s with 23.6psi vacuum 4.2m deep (21.0psia)
@13:04:00.90 Cartridge 18 filtering 378/1000ml at 0.20ml/s with 24.8psi vacuum 2.0m deep (17.7psia)
@13:05:00.90 Cartridge 18 filtering 391/1000ml at 0.23ml/s with 24.4psi vacuum 6.2m deep (23.8psia)
@13:06:00.90 Cartridge 18 filtering 404/1000ml at 0.22ml/s with 25.6psi vacuum 2.0m deep (17.7psia)
@13:07:00.90 Cartridge 18 filtering 417/1000ml at 0.20ml/s with 28.0psi vacuum 3.8m deep (20.4psia)
@13:08:00.90 Cartridge 18 filtering 430/1000ml at 0.20ml/s with 27.2psi vacuum 2.5m deep (18.5psia)
@13:09:00.90 Cartridge 18 filtering 443/1000ml at 0.20ml/s with 26.4psi vacuum
@13:10:00.90 Cartridge 18 filtering 455/1000ml at 0.20ml/s with 25.0psi vacuum 3.3m deep (19.6psia)
@13:11:00.90 Cartridge 18 filtering 468/1000ml at 0.21ml/s with 23.8psi vacuum 2.8m deep (18.8psia)
@13:12:00.90 Cartridge 18 filtering 480/1000ml at 0.20ml/s with 26.9psi vacuum 4.5m deep (21.4psia)
@13:13:00.90 Cartridge 18 filtering 492/1000ml at 0.22ml/s with 26.5psi vacuum 2.5m deep (18.4psia)
@13:13:51.83 <FILTERING> Flow averaged 0.25ml/s over 33:54
@13:13:51.86 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi
@13:13:52.44 Relieved excess vacuum in .6 seconds
@13:13:52.46 SamplePump.seek 1000ml,41:58
@13:14:00.18 Flow estimate reduced to 0.20ml/s
@13:14:00.19 <closeIntake> Intake.close
@13:14:00.25 <closeExhaust> Exhaust.close
@13:14:00.43 <FILTERING> Toroid.in :hold
@13:14:00.49 Toroid.seek :bypass
@13:14:00.89 <sampling> Cartridge 18 filtering 504/1000ml at 0.00ml/s with -0.3psi vacuum 5.9m deep (23.4psia)
@13:14:02.12 <FILTERING> Toroid.in :default
@13:14:02.18 Toroid.seek :clear
@13:14:03.79 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@13:14:03.80 Pressure.coast
@13:14:03.99 Sampled  504.4ml
@13:14:06.12 <LRAUV> -> Cmd.startProcessing
@13:14:06.49 <PROCESSING> Plunge.seek :reagent
@13:14:07.33 Sample loop closed. Ready to Deliver Reagents
@13:14:07.34 Archive.seek :sealed
@13:14:11.61 Archive.seek :evac
@13:14:25.54 delay 1:00
@13:15:25.57 Archive.seek :clear
@13:15:43.28 RNAlater soak for 10:00
@13:25:43.30 Archive.seek :tVent
@13:25:45.88 delay 1 second
@13:25:46.90 Archive.seek :evac
@13:26:02.53 delay 10 seconds
@13:26:12.56 Sample preserved and evacuated
@13:26:13.76 <LRAUV> -> Cmd.stop
@13:26:13.90 Carousel.seek 18
@13:26:14.07 Plunge.seek :locked
@13:26:15.01 <Archive> Archive.in :unlocking
@13:26:15.13 Archive.seek :clear
@13:26:15.21 <Air> Air.in :unlocking
@13:26:15.34 Air.seek :clear
@13:26:15.37 <Lysis1> Lysis1.in :unlocking
@13:26:15.53 Lysis1.seek :clear
@13:26:19.14 <LRAUV> Archive.coast
@13:26:19.20 Air.coast
@13:26:19.24 Lysis1.coast
@13:26:19.29 Lysis2.coast
@13:26:19.34 Plunge.coast
@13:26:19.39 SamplePump.coast
@13:26:19.44 Toroid.coast
@13:26:19.49 Carousel.coast
@13:26:19.55 PinPort.coast
@13:26:19.72 Gate.power :motor,:OFF
@13:26:19.73 Gate.power :core,:OFF
@13:26:19.76 Gate.power :analytic1,:OFF
@13:26:19.78 Gate.power :analytic2,:OFF
@13:26:19.80 Gate.power :analytic3,:OFF
@13:26:19.82 Gate.power :main,:OFF
@13:26:19.84 Safely stopped and ready to power off
##-- file closed 2021-04-24T20:38:46.978Z

##-- file opened 2021-04-24T20:38:46.980Z
@13:38:47.56 -> showStatus
@13:38:49.58 -> Cmd.loadCartridge -9
@13:38:49.72 Gate.power :main,:ON,limit to 5000mA
@13:38:49.74 Gate.power :analytic1,:ON,limit to 5000mA
@13:38:49.76 Gate.power :analytic2,:ON,limit to 5000mA
@13:38:49.78 Gate.power :analytic3,:ON,limit to 5000mA
@13:38:49.91 Powering core bus
@13:38:52.53 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@13:38:52.66 <LRAUV> Archive.configure :default
@13:38:52.79 Air.configure :default
@13:38:52.89 Lysis1.configure :default
@13:38:52.99 Lysis2.configure :default
@13:38:53.12 Plunge.configure :default
@13:38:53.24 SamplePump.configure :default
@13:38:53.39 ExtraPump.configure :default
@13:38:53.50 Toroid.configure :default
@13:38:53.63 Carousel.configure :default
@13:38:53.75 Pressure.configure :default
@13:38:53.86 PinPort.configure :default
@13:38:53.93 All elf controllers running firmware version 4.741
@13:38:53.95 Gate.power :motor,:ON,limit to 5000mA
@13:38:54.13 <Archive_home> Archive.in :default
@13:38:54.38 Archive.home.jog 0
@13:38:54.52 <Toroid_home> Toroid.in :default
@13:38:54.59 <Plunge_home> Plunge.in :default
@13:38:54.63 <Lysis2_home> Lysis2.in :default
@13:38:54.66 <Air_home> Air.in :default
@13:38:54.71 <PinPort_home> PinPort.in :default
@13:38:54.80 <Lysis1_home> Lysis1.in :default
@13:38:54.92 <Toroid_home> Toroid.home.jog 0
@13:38:55.00 <Lysis2_home> Lysis2.home.jog 0
@13:38:55.03 <PinPort_home> PinPort.home.jog 0
@13:38:55.06 <Plunge_home> Plunge.home.jog 0
@13:38:55.09 <Air_home> Air.home.jog 0
@13:38:55.12 <Lysis1_home> Lysis1.home.jog 0
@13:38:55.51 <Archive_home> Archive.in :homing
@13:38:55.80 <Toroid_home> Toroid.in :homing
@13:38:55.84 <Plunge_home> Plunge.in :homing
@13:38:55.87 <Air_home> Air.in :homing
@13:38:55.90 <Lysis1_home> Lysis1.in :homing
@13:38:55.94 <Archive_home> Archive.jog 150
@13:38:55.97 <PinPort_home> PinPort.in :homing
@13:38:56.00 <Lysis2_home> Lysis2.in :homing
@13:38:56.18 <Plunge_home> Plunge.jog 1581
@13:38:56.24 <Toroid_home> Toroid.jog 625
@13:38:56.28 <Air_home> Air.jog 163
@13:38:56.31 <PinPort_home> PinPort.jog -108
@13:38:56.34 <Lysis1_home> Lysis1.jog 1418
@13:38:56.40 <Lysis2_home> Lysis2.jog 1430
@13:38:56.63 <Archive_home> Archive.setRawPosition! 50
@13:38:56.68 Archive.seek :home
@13:38:56.76 <PinPort_home> PinPort.setRawPosition! 0
@13:38:56.81 PinPort.seek :home
@13:38:56.90 PinPort.in :default
@13:38:56.93 <Air_home> Air.setRawPosition! 50
@13:38:57.02 Air.seek :home
@13:38:57.10 <Archive_home> Archive.in :default
@13:38:57.30 <Air_home> Air.in :default
@13:38:57.58 <Plunge_home> Plunge.setRawPosition! 50
@13:38:57.63 Plunge.seek :home
@13:38:57.73 <Lysis1_home> Lysis1.setRawPosition! 50
@13:38:57.75 <Lysis2_home> Lysis2.setRawPosition! 50
@13:38:57.83 <Lysis1_home> Lysis1.seek :home
@13:38:57.85 <Lysis2_home> Lysis2.seek :home
@13:38:57.98 <Plunge_home> Plunge.in :default
@13:38:58.14 <Lysis1_home> Lysis1.in :default
@13:38:58.22 <Lysis2_home> Lysis2.in :default
@13:38:58.53 <Toroid_home> Toroid.setRawPosition! 0
@13:38:58.58 Toroid.seek :home
@13:38:58.67 Toroid.in :default
@13:38:58.80 <LRAUV> Toroid.seek :clear
@13:39:00.41 Carousel.in :default
@13:39:00.47 Carousel.home.jog 0
@13:39:00.96 Carousel.in :default
@13:39:01.02 Carousel.seek 18
@13:39:01.19 Selecting Cartridge 13
@13:39:01.43 Carousel.seek 13
@13:39:02.74 Lysis1.in :fast
@13:39:02.81 Lysis1.seek :locked
@13:39:05.40 <Plunge> Plunge.seek :locked
@13:39:05.53 <Air> Air.in :fast
@13:39:05.59 Air.seek :locked
@13:39:08.20 <LRAUV> Powering cartridge bus
@13:39:10.32 Gate.setIR 1750,5000,0,6
@13:39:10.45 Gate.setIR 0,5000,0,6
@13:39:10.51 PuckHeater.configure :default
@13:39:10.55 Gate.power :heat,:ON,limit to 5000mA
@13:39:11.23 Mounted lysis Cartridge board#Q05053
@13:39:11.26 Using default puck heat loss curve for HeatingAccelInCarousel
@13:39:29.98 -> Cmd.startFiltering
@13:39:30.43 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@13:39:30.45 SamplePump.setPosition! 0ml
@13:39:30.49 Bypass.open
@13:39:31.99 Intake.open
@13:39:32.80 Sea 4.8m deep (21.8psia)
@13:39:32.82 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@13:39:35.40 Vacuum tare is 51% of full scale
@13:39:35.53 Pressure.in :default
@13:39:35.59 Pressure.hold
@13:39:35.81 SamplePump.in :prime
@13:39:35.88 SamplePump.seek 10ml,1:21
@13:39:45.27 Pressure.coast
@13:39:45.32 SamplePump.setPosition! 0ml
@13:39:45.37 Exhaust.open
@13:39:46.78 <closeBypass> Bypass.close
@13:39:48.35 <FILTERING> Pressure.in :default
@13:39:48.41 Pressure.hold
@13:39:48.52 Sea 5.9m deep (23.4psia)
@13:39:48.65 SamplePump.in :prime
@13:39:48.72 SamplePump.seek 100ml,8:51
@13:41:58.89 Pressure.coast
@13:41:58.95 Bypass.open
@13:41:59.69 Intake.close
@13:42:00.54 Toroid.in :hold
@13:42:00.60 Toroid.seek :crack
@13:42:02.16 Toroid.seek :cartridge
@13:42:03.26 Toroid.in :default
@13:42:03.32 Toroid.seek :clear
@13:42:05.28 Pressurizing cartridge to 26psi above 23.7psia
@13:42:05.29 SamplePump.setPosition! 0ml
@13:42:05.33 Intake.open
@13:42:06.05 Bypass.close
@13:42:06.79 Exhaust.close
@13:42:07.60 Pressure.in :default
@13:42:07.66 Pressure.hold
@13:42:08.22 SamplePump.in :charge
@13:42:08.29 SamplePump.seek 2.3ml,42 seconds
@13:42:16.27 Pressure.coast
@13:42:16.32 Intake.close
@13:42:17.16 Pressurized cartridge with 0.281ml to 26.0psi
@13:42:17.17 Isolated @45.7psia (22.0psi)
@13:42:17.18 Verifying cartridge holds at least 0.75..0.85 of 45.7psia
@13:42:17.21 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@13:42:21.83 Sampling 1000.0ml
@13:42:21.85 SamplePump.setPosition! 0ml
@13:42:21.90 Exhaust.open
@13:42:22.63 Intake.open
@13:42:23.54 Pressure.in :default
@13:42:23.60 Pressure.hold
@13:42:23.71 Sea 7.3m deep (25.4psia)
@13:42:23.84 SamplePump.in :sample
@13:42:23.91 SamplePump.seek 1000ml,1:23:53
@13:42:26.58 <sampling> Cartridge 13 filtering 1/1000ml at 0.28ml/s with 21.4psi vacuum 5.5m deep (22.9psia)
@13:43:26.56 Cartridge 13 filtering 18/1000ml at 0.29ml/s with 28.8psi vacuum 1.9m deep (17.4psia)
@13:44:26.57 Cartridge 13 filtering 35/1000ml at 0.25ml/s with 27.2psi vacuum 2.8m deep (18.9psia)
@13:45:26.56 Cartridge 13 filtering 52/1000ml at 0.31ml/s with 22.3psi vacuum 3.9m deep (20.4psia)
@13:46:26.57 Cartridge 13 filtering 68/1000ml at 0.28ml/s with 21.9psi vacuum 3.5m deep (19.8psia)
@13:47:26.56 Cartridge 13 filtering 84/1000ml at 0.22ml/s with 22.6psi vacuum 4.8m deep (21.8psia)
@13:48:26.57 Cartridge 13 filtering 100/1000ml at 0.28ml/s with 25.9psi vacuum 2.4m deep (18.2psia)
@13:49:26.56 Cartridge 13 filtering 115/1000ml at 0.28ml/s with 23.7psi vacuum 5.2m deep (22.4psia)
@13:50:26.57 Cartridge 13 filtering 131/1000ml at 0.24ml/s with 21.2psi vacuum 3.3m deep (19.5psia)
@13:51:26.56 Cartridge 13 filtering 145/1000ml at 0.26ml/s with 23.5psi vacuum 4.4m deep (21.1psia)
@13:52:26.57 Cartridge 13 filtering 160/1000ml at 0.23ml/s with 27.4psi vacuum 3.5m deep (19.9psia)
@13:53:26.56 Cartridge 13 filtering 175/1000ml at 0.27ml/s with 22.7psi vacuum 2.7m deep (18.6psia)
@13:54:26.57 Cartridge 13 filtering 189/1000ml at 0.24ml/s with 23.0psi vacuum 5.2m deep (22.4psia)
@13:55:26.56 Cartridge 13 filtering 203/1000ml at 0.26ml/s with 25.5psi vacuum 1.9m deep (17.5psia)
@13:56:26.57 Cartridge 13 filtering 217/1000ml at 0.25ml/s with 23.4psi vacuum 3.8m deep (20.3psia)
@13:57:26.56 Cartridge 13 filtering 231/1000ml at 0.26ml/s with 23.3psi vacuum 3.1m deep (19.3psia)
@13:58:26.57 Cartridge 13 filtering 244/1000ml at 0.22ml/s with 22.7psi vacuum 3.6m deep (20.1psia)
@13:59:26.56 Cartridge 13 filtering 258/1000ml at 0.22ml/s with 22.4psi vacuum 4.4m deep (21.2psia)
@14:00:26.57 Cartridge 13 filtering 271/1000ml at 0.20ml/s with 25.8psi vacuum 1.8m deep (17.4psia)
@14:01:26.56 Cartridge 13 filtering 284/1000ml at 0.21ml/s with 23.3psi vacuum 4.8m deep (21.8psia)
@14:02:26.57 Cartridge 13 filtering 297/1000ml at 0.20ml/s with 25.8psi vacuum 2.1m deep (17.8psia)
@14:03:26.56 Cartridge 13 filtering 310/1000ml at 0.21ml/s with 23.3psi vacuum 4.1m deep (20.8psia)
@14:04:26.57 Cartridge 13 filtering 323/1000ml at 0.21ml/s with 23.7psi vacuum 4.0m deep (20.6psia)
@14:05:26.56 Cartridge 13 filtering 335/1000ml at 0.20ml/s with 23.9psi vacuum 2.3m deep (18.1psia)
@14:06:26.57 Cartridge 13 filtering 348/1000ml at 0.23ml/s with 24.8psi vacuum 5.2m deep (22.4psia)
@14:07:26.56 Cartridge 13 filtering 360/1000ml at 0.22ml/s with 25.7psi vacuum 2.7m deep (18.7psia)
@14:08:26.57 Cartridge 13 filtering 373/1000ml at 0.22ml/s with 22.7psi vacuum 5.6m deep (22.9psia)
@14:09:26.56 Cartridge 13 filtering 385/1000ml at 0.20ml/s with 27.6psi vacuum 1.8m deep (17.3psia)
@14:10:26.57 Cartridge 13 filtering 397/1000ml at 0.20ml/s with 24.6psi vacuum 4.3m deep (21.0psia)
@14:11:26.56 Cartridge 13 filtering 410/1000ml at 0.20ml/s with 24.7psi vacuum 2.3m deep (18.1psia)
@14:12:04.98 <FILTERING> Flow averaged 0.23ml/s over 29:42
@14:12:05.01 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi
@14:12:05.58 Relieved excess vacuum in .6 seconds
@14:12:05.61 SamplePump.seek 1000ml,49:05
@14:12:26.57 <sampling> Cartridge 13 filtering 422/1000ml at 0.20ml/s with 29.5psi vacuum 2.6m deep (18.5psia)
@14:12:50.99 <FILTERING> Flow averaged 0.20ml/s over 46 seconds
@14:12:51.02 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi
@14:12:51.59 Relieved excess vacuum in .6 seconds
@14:12:51.62 SamplePump.seek 1000ml,48:19
@14:12:56.35 Flow estimate reduced to 0.16ml/s
@14:12:56.37 <closeIntake> Intake.close
@14:12:56.42 <closeExhaust> Exhaust.close
@14:12:56.60 <FILTERING> Toroid.in :hold
@14:12:56.66 Toroid.seek :bypass
@14:12:58.29 Toroid.in :default
@14:12:58.35 Toroid.seek :clear
@14:12:59.97 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@14:12:59.98 Pressure.coast
@14:13:00.17 Sampled  427.5ml
@14:13:02.39 <LRAUV> -> Cmd.startProcessing
@14:13:03.82 <PROCESSING> SPR.command \"read_sequences\"
@14:13:04.55 SPR.command \"stats_on\"
@14:13:04.59 SPR.command \"start 0 1\"
@14:13:06.60 <SPRlogger> Begun log /var/log/esp/spr21apr2414h.spr
@14:13:07.41 <PROCESSING> Handoff.select :waste
@14:13:14.09 Syringe tare is 20% of full scale
@14:13:14.11 PinPort.in :locking
@14:13:14.18 PinPort.seek :locked
@14:13:14.66 <posArchive> Archive.in :fast
@14:13:14.72 <posLyS2> Lysis2.in :fast
@14:13:14.77 <PROCESSING> Lysis1.seek 1350uL
@14:13:14.88 <posArchive> Archive.seek :full
@14:13:14.93 <posLyS2> Lysis2.seek :full
@14:13:17.01 <PROCESSING> Plunge.seek :reagent
@14:13:17.92 Evacuating puck with filter bubble point of 50psi
@14:13:17.94 PinPort.seek :locked
@14:13:18.02 Handoff.select :waste
@14:13:25.60 Air syringe pumping up to 1 stroke to reach 8psi
@14:13:25.61 Air stroke #1 of 1
@14:13:25.69 Air.in :pressurize
@14:13:25.75 Air.seek :pressurized
@14:13:37.02 -0.1psi after pressurize
@14:13:37.03 Air.in :compress
@14:13:37.11 Air.seek :evac
@14:13:41.20 0.2psi after compress
@14:13:53.78 -0.0psi after compress (stable)
@14:13:53.79 Air.in :default
@14:13:53.86 Air.hold
@14:13:54.03 at 0.0psi Opened to waste
@14:13:54.04 Handoff.select :blocked
@14:13:54.18 Archive syringe pumping up to 5 strokes to reach 50psi
@14:13:54.21 Archive stroke #1 of 5
@14:13:54.29 Archive.in :pressurize
@14:13:54.36 Archive.seek :pressurized
@14:14:07.11 5.3psi after pressurize
@14:14:07.12 Archive.in :compress
@14:14:07.20 Archive.seek :empty
@14:14:23.09 31.6psi after compress
@14:14:35.75 Reached 50.0 of 50.0psi after pumping 1730uL (1.00 strokes)
@14:14:35.76 Archive.in :default
@14:14:35.85 Archive.hold
@14:14:35.91 Handoff.select :waste
@14:14:46.09 delay 1:00
@14:15:46.12 Handoff.select :blocked
@14:16:08.80 Handoff.select :waste
@14:16:08.92 0.3psi at evacEnd
@14:16:08.93 Handoff.select :blocked
@14:16:09.06 Archive.in :fast
@14:16:09.13 Archive.seek :topVent
@14:16:22.85 Stable @0.7psi after Handoff blocked
@14:16:22.87 Archive syringe pumping up to 5 strokes to reach 31psi
@14:16:22.88 Archive stroke #1 of 5
@14:16:22.98 Archive.in :pressurize
@14:16:23.04 Archive.seek :pressurized
@14:16:35.79 0.9psi after pressurize
@14:16:35.80 Archive.in :compress
@14:16:35.88 Archive.seek :empty
@14:16:52.22 1.0psi after compress
@14:17:04.79 1.1psi after compress (stable)
@14:17:04.81 Archive.in :gulp
@14:17:04.88 Archive.seek :topVent
@14:17:12.08 1.6psi after gulp
@14:17:24.65 1.6psi after gulp (stable)
@14:17:24.66 Archive stroke #2 of 5
@14:17:24.75 Archive.in :pressurize
@14:17:24.81 Archive.seek :pressurized
@14:17:37.56 1.6psi after pressurize
@14:17:37.58 Archive.in :compress
@14:17:37.65 Archive.seek :empty
@14:17:53.57 1.5psi after compress
@14:18:06.15 2.4psi after compress (stable)
@14:18:06.16 Archive.in :gulp
@14:18:06.23 Archive.seek :topVent
@14:18:13.45 4.2psi after gulp
@14:18:58.52 10.7psi after gulp (stable)
@14:18:58.53 Archive stroke #3 of 5
@14:18:58.62 Archive.in :pressurize
@14:18:58.68 Archive.seek :pressurized
@14:19:11.42 12.4psi after pressurize
@14:19:11.44 Archive.in :compress
@14:19:11.51 Archive.seek :empty
@14:19:27.40 13.8psi after compress
@14:19:56.15 Reached 30.8 of 30.7psi after pumping 5190uL (3.00 strokes)
@14:19:56.17 Archive.in :default
@14:19:56.24 Archive.hold
@14:19:56.33 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position
@14:19:56.35 Handoff.select :waste
@14:20:12.58 delay 1:00
@14:21:12.61 Handoff.select :blocked
@14:21:48.29 Handoff.select :waste
@14:21:48.41 6.9psi at evacEnd
@14:21:48.43 Air.seek :fullVent
@14:21:49.88 Archive.in :fast
@14:21:49.94 Archive.seek :topVent
@14:21:57.11 Seawater evacuated, Presure above/below filter released. Lysis begins
@14:21:57.20 Lysis1.in :slow
@14:21:57.26 Lysis1.seek 1050uL
@14:22:27.30 delay 5 seconds
@14:22:32.32 Handoff.select :blocked
@14:22:32.49 Lysis1.seek 450uL
@14:23:32.53 delay 3 seconds
@14:23:35.60 Air.in to mix into 1.37806 parts Lysis1
@14:23:36.08 <Airmixer> Air.seek :evac
@14:23:36.12 <PROCESSING> Lysis1.seek 300uL
@14:23:51.24 Lysis1.seek :empty
@14:24:21.37 Lysis buffer delivered @24.9psi
@14:24:21.38 Polling Can every 2:00
@14:24:21.93 PuckHeater.configure :default #ambient 11.3C
@14:24:21.97 PuckHeater.seek 80.2C
@14:25:30.65 PuckHeater.hold 2:00,80.2C
@14:27:30.61 Polling Can every 2:00
@14:27:31.16 PuckHeater.configure :default #ambient 11.4C
@14:27:31.20 PuckHeater.hold 30 seconds,79.2C
@14:28:01.36 Polling Can every 2:00
@14:28:01.91 PuckHeater.hold 30 seconds,78.7C
@14:28:32.07 Polling Can every 2:00
@14:28:32.62 PuckHeater.hold 10:00,78.1C
@14:38:31.74 Lysis complete. Syringe pressure @32.9psi
@14:38:31.75 Handoff.select :blocked
@14:38:31.79 Archive syringe pumping up to 3 strokes to reach 40psi
@14:38:31.80 Archive stroke #1 of 3
@14:38:31.89 Archive.in :pressurize
@14:38:31.96 Archive.seek :pressurized
@14:38:44.70 32.5psi after pressurize
@14:38:44.71 Archive.in :compress
@14:38:44.79 Archive.seek :empty
@14:38:55.38 Reached 41.2 of 40.0psi after pumping 1617uL (0.93 strokes)
@14:38:55.39 Archive.in :default
@14:38:55.46 Archive.hold
@14:38:55.55 delay 2 seconds
@14:38:57.57 Archive.in :fast
@14:38:57.63 Archive.seek :full
@14:39:04.44 Lysis1.seek 138uL
@14:39:18.33 delay 1 second
@14:39:19.36 <fillArchive> Archive.seek :full
@14:39:19.38 <PROCESSING> Lysis1.seek :empty
@14:39:33.37 Initial air pressure @53.1psi
@14:39:33.43 Lysis1.in :extract
@14:39:33.52 Lysis1.seek 600uL,nil
@14:39:35.19 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@14:39:35.20 Archive stroke #1 of 6
@14:39:35.33 Archive.in :pressurize
@14:39:35.39 Archive.seek :pressurized
@14:39:48.14 15.8psi after pressurize
@14:39:48.15 Archive.in :compress
@14:39:48.23 Archive.seek :empty
@14:40:01.98 Syringe::LysatePlungerStuck in PROCESSING -- at < 273uL during Lysis Syringe #1 (LyS1) move
@14:40:02.01 <PROCESSING> Lysis1.in :default
@14:40:02.08 Lysis1.seek 238uL
@14:40:02.79 <pump> Reached 29.7 of 25.0psi after pumping 1702uL (0.98 strokes)
@14:40:02.80 Archive.in :default
@14:40:02.87 Archive.hold
@14:40:03.89 <PROCESSING> Lysis1.in :extract
@14:40:03.96 Lysis1.seek 600uL,nil
@14:40:07.52 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@14:40:07.53 Archive stroke #1 of 6
@14:40:07.62 Archive.in :compress
@14:40:07.69 Archive.seek :empty
@14:40:09.28 16.0psi after compress
@14:40:16.35 Archive.in :default
@14:40:16.40 Archive.hold
@14:40:16.52 <PROCESSING> Initial 600uL lysate @16.1psi
@14:40:16.53 Handoff.select :blocked
@14:40:16.64 Initial air pressure @16.4psi
@14:40:16.72 Lysis1.seek 825uL,nil
@14:40:17.36 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@14:40:17.37 Archive stroke #1 of 6
@14:40:17.46 Archive.in :compress
@14:40:17.53 Archive.seek :empty
@14:40:17.73 14.9psi after compress
@14:40:30.31 15.8psi after compress (stable)
@14:40:35.44 Recovering 2.9uL/s
@14:40:40.56 Recovering 2.1uL/s
@14:40:45.63 Archive.in :gulp
@14:40:45.69 Archive.seek :topVent
@14:40:52.90 15.6psi after gulp
@14:41:05.47 15.8psi after gulp (stable)
@14:41:05.48 Archive stroke #2 of 6
@14:41:05.57 Archive.in :pressurize
@14:41:05.64 Archive.seek :pressurized
@14:41:18.40 15.3psi after pressurize
@14:41:18.41 Archive.in :compress
@14:41:18.48 Archive.seek :empty
@14:41:34.02 Archive.in :default
@14:41:34.07 Archive.hold
@14:41:34.35 <PROCESSING> Recovered 825uL lysate @16.7psi
@14:41:34.36 Air.in :halfSpeed
@14:41:34.43 Air.seek :fullVent
@14:41:36.67 Puck Top Vented, bottom @7.0psi
@14:41:36.68 Handoff.select :waste
@14:41:36.80 delay 5 seconds
@14:41:41.86 Handoff open to waste, bottom @0.1psi
@14:41:41.88 Air.in :default
@14:41:41.95 Air.seek :evac
@14:41:43.41 Lysis2.in :DAmix
@14:41:43.55 Lysis1.in :DAmix
@14:41:44.10 <Lysis2mixer> Lysis2.seek 1480uL
@14:41:44.14 <PROCESSING> Lysis1.seek 575uL
@14:42:19.23 delay 5 seconds
@14:42:24.25 Handoff.select SPR
@14:42:24.38 SPR.command \"start 2 14\"
@14:42:25.81 delay 10 seconds
@14:42:35.83 SPR.command \"start 1 0\"
@14:42:39.06 Waiting up to 4:00 for SPR to enter state 1
@14:44:41.43 SPR.command \"hold_on\"
@14:44:42.84 SPR Dilution 1 of 1 -- Running Experiment 1
@14:44:43.31 <Lysis2mixer> Lysis2.seek 1180uL
@14:44:43.38 <PROCESSING> Lysis1.seek 275uL
@14:45:25.38 volL1 = 300.0 and volL2 = 300.0
@14:45:25.39 delay 15 seconds
@14:45:40.41 SPR.command \"hold_off\"
@14:45:43.50 Waiting up to 38:00 for SPR to enter state 7
@15:12:27.04 SPR Analysis has Completed
@15:12:27.06 Handoff.select :waste
@15:12:27.98 <SPRlogger> Ended log /var/log/esp/spr21apr2414h.spr
@15:12:27.99 <PROCESSING> SPR.command \"stats_off\"
@15:12:29.47 <LRAUV> -> Cmd.stop
@15:12:29.49 Archive.stop
@15:12:29.62 <closeHandoff> Handoff.select :close
@15:12:29.75 <LRAUV> Gate.power :heat,:OFF
@15:12:29.77 Gate.power :cartridge,:OFF
@15:12:29.86 Carousel.seek 13
@15:12:30.03 Plunge.seek :locked
@15:12:30.92 PinPort.in :unlocking
@15:12:30.98 PinPort.seek :clear
@15:12:31.52 <Archive> Archive.in :unlocking
@15:12:31.65 Archive.seek :clear
@15:12:31.71 <Air> Air.in :unlocking
@15:12:31.84 Air.seek :clear
@15:12:31.91 <Lysis1> Lysis1.in :unlocking
@15:12:32.02 Lysis1.seek :clear
@15:12:32.09 <Lysis2> Lysis2.in :unlocking
@15:12:32.16 Lysis2.seek :clear
@15:12:35.70 <LRAUV> Archive.coast
@15:12:35.75 Air.coast
@15:12:35.80 Lysis1.coast
@15:12:35.85 Lysis2.coast
@15:12:35.90 Plunge.coast
@15:12:35.95 SamplePump.coast
@15:12:36.00 Toroid.coast
@15:12:36.05 Carousel.coast
@15:12:36.10 PinPort.coast
@15:12:36.29 Gate.power :motor,:OFF
@15:12:36.31 Gate.power :core,:OFF
@15:12:36.33 Gate.power :analytic1,:OFF
@15:12:36.35 Gate.power :analytic2,:OFF
@15:12:36.37 Gate.power :analytic3,:OFF
@15:12:36.39 Gate.power :main,:OFF
@15:12:36.41 Safely stopped and ready to power off
##-- file closed 2021-04-25T00:22:30.978Z

##-- file opened 2021-04-25T00:22:30.991Z
@17:22:30.87 -> showStatus
@17:22:32.89 -> Cmd.loadCartridge -6
@17:22:33.03 Gate.power :main,:ON,limit to 5000mA
@17:22:33.04 Gate.power :analytic1,:ON,limit to 5000mA
@17:22:33.07 Gate.power :analytic2,:ON,limit to 5000mA
@17:22:33.09 Gate.power :analytic3,:ON,limit to 5000mA
@17:22:33.22 Powering core bus
@17:22:35.91 Archive.configure :default
@17:22:36.03 Air.configure :default
@17:22:36.14 Lysis1.configure :default
@17:22:36.24 Lysis2.configure :default
@17:22:36.38 Plunge.configure :default
@17:22:36.45 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@17:22:36.55 <LRAUV> SamplePump.configure :default
@17:22:36.70 ExtraPump.configure :default
@17:22:36.82 Toroid.configure :default
@17:22:36.94 Carousel.configure :default
@17:22:37.07 Pressure.configure :default
@17:22:37.18 PinPort.configure :default
@17:22:37.24 All elf controllers running firmware version 4.741
@17:22:37.26 Gate.power :motor,:ON,limit to 5000mA
@17:22:37.45 <Archive_home> Archive.in :default
@17:22:37.67 Archive.home.jog 0
@17:22:37.81 <Toroid_home> Toroid.in :default
@17:22:37.89 <Plunge_home> Plunge.in :default
@17:22:37.92 <Lysis1_home> Lysis1.in :default
@17:22:37.97 <PinPort_home> PinPort.in :default
@17:22:38.03 <Lysis2_home> Lysis2.in :default
@17:22:38.06 <Air_home> Air.in :default
@17:22:38.25 <Plunge_home> Plunge.home.jog 0
@17:22:38.28 <Toroid_home> Toroid.home.jog 0
@17:22:38.30 <Lysis1_home> Lysis1.home.jog 0
@17:22:38.33 <Lysis2_home> Lysis2.home.jog 0
@17:22:38.35 <PinPort_home> PinPort.home.jog 0
@17:22:38.39 <Air_home> Air.home.jog 0
@17:22:38.83 <Archive_home> Archive.in :homing
@17:22:39.04 <Toroid_home> Toroid.in :homing
@17:22:39.07 <Plunge_home> Plunge.in :homing
@17:22:39.11 <Air_home> Air.in :homing
@17:22:39.14 <Lysis1_home> Lysis1.in :homing
@17:22:39.18 <Archive_home> Archive.jog 154
@17:22:39.21 <PinPort_home> PinPort.in :homing
@17:22:39.26 <Lysis2_home> Lysis2.in :homing
@17:22:39.44 <Toroid_home> Toroid.jog 623
@17:22:39.49 <Plunge_home> Plunge.jog 1575
@17:22:39.53 <Air_home> Air.jog 158
@17:22:39.56 <PinPort_home> PinPort.jog -107
@17:22:39.59 <Lysis1_home> Lysis1.jog 1416
@17:22:39.63 <Lysis2_home> Lysis2.jog 1430
@17:22:39.85 <Archive_home> Archive.setRawPosition! 50
@17:22:39.89 Archive.seek :home
@17:22:39.98 <PinPort_home> PinPort.setRawPosition! 0
@17:22:40.02 PinPort.seek :home
@17:22:40.13 PinPort.in :default
@17:22:40.15 <Air_home> Air.setRawPosition! 50
@17:22:40.25 Air.seek :home
@17:22:40.33 <Archive_home> Archive.in :default
@17:22:40.53 <Air_home> Air.in :default
@17:22:40.90 <Plunge_home> Plunge.setRawPosition! 50
@17:22:40.94 Plunge.seek :home
@17:22:41.03 <Lysis2_home> Lysis2.setRawPosition! 50
@17:22:41.05 <Lysis1_home> Lysis1.setRawPosition! 50
@17:22:41.12 <Lysis2_home> Lysis2.seek :home
@17:22:41.17 <Lysis1_home> Lysis1.seek :home
@17:22:41.29 <Plunge_home> Plunge.in :default
@17:22:41.42 <Lysis2_home> Lysis2.in :default
@17:22:41.51 <Lysis1_home> Lysis1.in :default
@17:22:41.72 <Toroid_home> Toroid.setRawPosition! 0
@17:22:41.77 Toroid.seek :home
@17:22:41.85 Toroid.in :default
@17:22:41.99 <LRAUV> Toroid.seek :clear
@17:22:43.62 Carousel.in :default
@17:22:43.68 Carousel.home.jog 0
@17:22:44.18 Carousel.in :default
@17:22:44.25 Carousel.seek 13
@17:22:44.41 Selecting Cartridge 16
@17:22:44.65 Carousel.seek 16
@17:22:45.54 Lysis1.in :fast
@17:22:45.60 Lysis1.seek :locked
@17:22:48.20 <Plunge> Plunge.seek :locked
@17:22:48.34 <Air> Air.in :fast
@17:22:48.41 Air.seek :locked
@17:22:51.01 <LRAUV> Powering cartridge bus
@17:22:53.14 Gate.setIR 1750,5000,0,6
@17:22:53.27 Gate.setIR 0,5000,0,6
@17:22:53.32 Gate.power :cartridge,:OFF
@17:22:53.86 Mounted archive Cartridge
@17:23:12.49 -> Cmd.startFiltering
@17:23:12.94 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@17:23:12.96 SamplePump.setPosition! 0ml
@17:23:13.00 Bypass.open
@17:23:14.48 Intake.open
@17:23:15.25 Sea 5.7m deep (23.2psia)
@17:23:15.27 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@17:23:17.84 Vacuum tare is 51% of full scale
@17:23:17.96 Pressure.in :default
@17:23:18.02 Pressure.hold
@17:23:18.19 SamplePump.in :prime
@17:23:18.26 SamplePump.seek 10ml,1:21
@17:23:27.65 Pressure.coast
@17:23:27.70 SamplePump.setPosition! 0ml
@17:23:27.75 Exhaust.open
@17:23:29.19 <closeBypass> Bypass.close
@17:23:30.77 <FILTERING> Pressure.in :default
@17:23:30.83 Pressure.hold
@17:23:30.94 Sea 6.8m deep (24.8psia)
@17:23:31.07 SamplePump.in :prime
@17:23:31.14 SamplePump.seek 100ml,8:51
@17:25:40.80 Pressure.coast
@17:25:40.86 Bypass.open
@17:25:41.58 Intake.close
@17:25:42.41 Toroid.in :hold
@17:25:42.47 Toroid.seek :crack
@17:25:44.03 Toroid.seek :cartridge
@17:25:45.12 Toroid.in :default
@17:25:45.18 Toroid.seek :clear
@17:25:47.14 Pressurizing cartridge to 26psi above 24.4psia
@17:25:47.15 SamplePump.setPosition! 0ml
@17:25:47.19 Intake.open
@17:25:47.92 Bypass.close
@17:25:48.66 Exhaust.close
@17:25:49.46 Pressure.in :default
@17:25:49.52 Pressure.hold
@17:25:50.08 SamplePump.in :charge
@17:25:50.15 SamplePump.seek 2.3ml,42 seconds
@17:25:56.17 Pressure.coast
@17:25:56.22 Intake.close
@17:25:57.06 Pressurized cartridge with 0.159ml to 26.6psi
@17:25:57.07 Isolated @45.4psia (21.0psi)
@17:25:57.08 Verifying cartridge holds at least 0.75..0.85 of 45.4psia
@17:25:57.11 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@17:26:01.68 Cartridge pressure fell 1% to 45.1psia
@17:26:01.76 Sampling 1000.0ml
@17:26:01.77 SamplePump.setPosition! 0ml
@17:26:01.82 Exhaust.open
@17:26:02.54 Intake.open
@17:26:03.41 Pressure.in :default
@17:26:03.47 Pressure.hold
@17:26:03.59 Sea 6.9m deep (24.9psia)
@17:26:03.72 SamplePump.in :sample
@17:26:03.79 SamplePump.seek 1000ml,1:23:53
@17:26:06.45 <sampling> Cartridge 16 filtering 1/1000ml at 0.28ml/s with 26.7psi vacuum 3.6m deep (20.0psia)
@17:27:06.44 Cartridge 16 filtering 19/1000ml at 0.34ml/s with 26.4psi vacuum
@17:28:06.45 Cartridge 16 filtering 38/1000ml at 0.34ml/s with 23.1psi vacuum 4.8m deep (21.7psia)
@17:29:06.44 Cartridge 16 filtering 56/1000ml at 0.32ml/s with 26.7psi vacuum 3.5m deep (19.8psia)
@17:30:06.45 Cartridge 16 filtering 74/1000ml at 0.25ml/s with 26.5psi vacuum 2.4m deep (18.3psia)
@17:31:06.45 Cartridge 16 filtering 92/1000ml at 0.30ml/s with 21.6psi vacuum 3.5m deep (19.9psia)
@17:32:06.45 Cartridge 16 filtering 109/1000ml at 0.32ml/s with 27.1psi vacuum 3.4m deep (19.8psia)
@17:33:06.45 Cartridge 16 filtering 127/1000ml at 0.31ml/s with 24.8psi vacuum 2.0m deep (17.7psia)
@17:34:06.45 Cartridge 16 filtering 144/1000ml at 0.30ml/s with 22.8psi vacuum 2.9m deep (19.0psia)
@17:35:06.45 Cartridge 16 filtering 160/1000ml at 0.31ml/s with 23.9psi vacuum 4.6m deep (21.6psia)
@17:36:06.45 Cartridge 16 filtering 177/1000ml at 0.29ml/s with 21.6psi vacuum 3.0m deep (19.2psia)
@17:37:06.45 Cartridge 16 filtering 193/1000ml at 0.28ml/s with 21.3psi vacuum 6.0m deep (23.5psia)
@17:38:06.45 Cartridge 16 filtering 210/1000ml at 0.30ml/s with 23.6psi vacuum 3.5m deep (19.8psia)
@17:39:06.44 Cartridge 16 filtering 226/1000ml at 0.28ml/s with 23.4psi vacuum 5.0m deep (22.0psia)
@17:40:06.45 Cartridge 16 filtering 242/1000ml at 0.29ml/s with 20.2psi vacuum 4.3m deep (21.1psia)
@17:41:06.44 Cartridge 16 filtering 257/1000ml at 0.22ml/s with 21.3psi vacuum 3.6m deep (20.0psia)
@17:42:06.45 Cartridge 16 filtering 273/1000ml at 0.22ml/s with 21.3psi vacuum 4.3m deep (21.1psia)
@17:43:06.44 Cartridge 16 filtering 288/1000ml at 0.27ml/s with 25.3psi vacuum
@17:44:06.45 Cartridge 16 filtering 303/1000ml at 0.27ml/s with 23.8psi vacuum 5.3m deep (22.5psia)
@17:45:06.45 Cartridge 16 filtering 318/1000ml at 0.28ml/s with 25.6psi vacuum 2.8m deep (18.8psia)
@17:46:06.45 Cartridge 16 filtering 333/1000ml at 0.26ml/s with 25.1psi vacuum 2.8m deep (18.9psia)
@17:47:06.45 Cartridge 16 filtering 348/1000ml at 0.20ml/s with 24.6psi vacuum
@17:48:06.45 Cartridge 16 filtering 362/1000ml at 0.23ml/s with 26.6psi vacuum 3.4m deep (19.7psia)
@17:49:06.45 Cartridge 16 filtering 377/1000ml at 0.26ml/s with 25.6psi vacuum 4.3m deep (21.0psia)
@17:50:06.45 Cartridge 16 filtering 391/1000ml at 0.24ml/s with 25.2psi vacuum 1.6m deep (17.1psia)
@17:51:06.45 Cartridge 16 filtering 405/1000ml at 0.21ml/s with 23.1psi vacuum 4.7m deep (21.7psia)
@17:52:06.45 Cartridge 16 filtering 419/1000ml at 0.24ml/s with 22.1psi vacuum 3.4m deep (19.7psia)
@17:53:06.45 Cartridge 16 filtering 433/1000ml at 0.22ml/s with 26.7psi vacuum 5.1m deep (22.2psia)
@17:54:06.45 Cartridge 16 filtering 447/1000ml at 0.21ml/s with 23.1psi vacuum
@17:55:06.45 Cartridge 16 filtering 461/1000ml at 0.20ml/s with 24.8psi vacuum 3.1m deep (19.2psia)
@17:56:06.45 Cartridge 16 filtering 474/1000ml at 0.23ml/s with 25.1psi vacuum 3.5m deep (19.9psia)
@17:57:06.45 Cartridge 16 filtering 488/1000ml at 0.25ml/s with 20.5psi vacuum 3.9m deep (20.5psia)
@17:58:06.45 Cartridge 16 filtering 501/1000ml at 0.23ml/s with 23.5psi vacuum 5.5m deep (22.8psia)
@17:59:06.45 Cartridge 16 filtering 514/1000ml at 0.25ml/s with 25.5psi vacuum 1.9m deep (17.6psia)
@18:00:06.45 Cartridge 16 filtering 527/1000ml at 0.24ml/s with 19.3psi vacuum 7.4m deep (25.7psia)
@18:01:06.45 Cartridge 16 filtering 540/1000ml at 0.22ml/s with 23.7psi vacuum 2.9m deep (18.9psia)
@18:02:06.45 Cartridge 16 filtering 553/1000ml at 0.21ml/s with 23.6psi vacuum 2.8m deep (18.8psia)
@18:03:06.45 Cartridge 16 filtering 566/1000ml at 0.22ml/s with 19.8psi vacuum 3.7m deep (20.2psia)
@18:04:06.45 Cartridge 16 filtering 578/1000ml at 0.21ml/s with 25.3psi vacuum 4.0m deep (20.5psia)
@18:05:06.45 Cartridge 16 filtering 591/1000ml at 0.24ml/s with 25.2psi vacuum 4.4m deep (21.2psia)
@18:06:06.45 Cartridge 16 filtering 603/1000ml at 0.20ml/s with 24.1psi vacuum 3.7m deep (20.1psia)
@18:07:06.45 Cartridge 16 filtering 616/1000ml at 0.21ml/s with 25.8psi vacuum 4.5m deep (21.3psia)
@18:08:06.45 Cartridge 16 filtering 628/1000ml at 0.20ml/s with 25.5psi vacuum
@18:09:06.45 Cartridge 16 filtering 641/1000ml at 0.20ml/s with 27.6psi vacuum 4.4m deep (21.2psia)
@18:10:06.45 Cartridge 16 filtering 653/1000ml at 0.21ml/s with 19.3psi vacuum 3.6m deep (20.0psia)
@18:11:06.45 Cartridge 16 filtering 665/1000ml at 0.20ml/s with 29.4psi vacuum 5.0m deep (22.0psia)
@18:11:12.72 <FILTERING> Flow averaged 0.25ml/s over 45:09
@18:11:12.75 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi
@18:11:13.32 Relieved excess vacuum in .6 seconds
@18:11:13.35 SamplePump.seek 1000ml,28:19
@18:11:19.61 Flow estimate reduced to 0.20ml/s
@18:11:19.64 <closeIntake> Intake.close
@18:11:19.69 <closeExhaust> Exhaust.close
@18:11:19.86 <FILTERING> Toroid.in :hold
@18:11:19.92 Toroid.seek :bypass
@18:11:21.56 Toroid.in :default
@18:11:21.62 Toroid.seek :clear
@18:11:23.23 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@18:11:23.24 Pressure.coast
@18:11:23.43 Sampled  667.6ml
@18:11:25.30 <LRAUV> -> Cmd.startProcessing
@18:11:25.68 <PROCESSING> Plunge.seek :reagent
@18:11:26.52 Sample loop closed. Ready to Deliver Reagents
@18:11:26.53 Archive.seek :sealed
@18:11:30.80 Archive.seek :evac
@18:11:44.75 delay 1:00
@18:12:44.78 Archive.seek :clear
@18:13:02.51 RNAlater soak for 10:00
@18:23:02.54 Archive.seek :tVent
@18:23:05.11 delay 1 second
@18:23:06.13 Archive.seek :evac
@18:23:21.76 delay 10 seconds
@18:23:31.79 Sample preserved and evacuated
@18:23:32.96 <LRAUV> -> Cmd.stop
@18:23:33.10 Carousel.seek 16
@18:23:33.27 Plunge.seek :locked
@18:23:34.22 <Archive> Archive.in :unlocking
@18:23:34.34 Archive.seek :clear
@18:23:34.42 <Air> Air.in :unlocking
@18:23:34.53 Air.seek :clear
@18:23:34.56 <Lysis1> Lysis1.in :unlocking
@18:23:34.72 Lysis1.seek :clear
@18:23:38.38 <LRAUV> Archive.coast
@18:23:38.43 Air.coast
@18:23:38.48 Lysis1.coast
@18:23:38.53 Lysis2.coast
@18:23:38.59 Plunge.coast
@18:23:38.63 SamplePump.coast
@18:23:38.68 Toroid.coast
@18:23:38.74 Carousel.coast
@18:23:38.79 PinPort.coast
@18:23:38.97 Gate.power :motor,:OFF
@18:23:38.98 Gate.power :core,:OFF
@18:23:39.01 Gate.power :analytic1,:OFF
@18:23:39.03 Gate.power :analytic2,:OFF
@18:23:39.05 Gate.power :analytic3,:OFF
@18:23:39.07 Gate.power :main,:OFF
@18:23:39.09 Safely stopped and ready to power off
##-- file closed 2021-04-25T01:35:32.532Z

##-- file opened 2021-04-25T01:35:32.539Z
@18:35:32.86 -> showStatus
@18:35:34.89 -> Cmd.loadCartridge -9
@18:35:35.03 Gate.power :main,:ON,limit to 5000mA
@18:35:35.05 Gate.power :analytic1,:ON,limit to 5000mA
@18:35:35.08 Gate.power :analytic2,:ON,limit to 5000mA
@18:35:35.10 Gate.power :analytic3,:ON,limit to 5000mA
@18:35:35.58 Powering core bus
@18:35:37.52 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@18:35:37.82 <LRAUV> Archive.configure :default
@18:35:37.94 Air.configure :default
@18:35:38.05 Lysis1.configure :default
@18:35:38.16 Lysis2.configure :default
@18:35:38.28 Plunge.configure :default
@18:35:38.40 SamplePump.configure :default
@18:35:38.55 ExtraPump.configure :default
@18:35:38.66 Toroid.configure :default
@18:35:38.78 Carousel.configure :default
@18:35:38.91 Pressure.configure :default
@18:35:39.02 PinPort.configure :default
@18:35:39.09 All elf controllers running firmware version 4.741
@18:35:39.12 Gate.power :motor,:ON,limit to 5000mA
@18:35:39.29 <Archive_home> Archive.in :default
@18:35:39.57 Archive.home.jog 0
@18:35:39.73 <Toroid_home> Toroid.in :default
@18:35:39.81 <Plunge_home> Plunge.in :default
@18:35:39.84 <Lysis1_home> Lysis1.in :default
@18:35:39.89 <PinPort_home> PinPort.in :default
@18:35:39.94 <Lysis2_home> Lysis2.in :default
@18:35:39.98 <Air_home> Air.in :default
@18:35:40.18 <Plunge_home> Plunge.home.jog 0
@18:35:40.20 <Toroid_home> Toroid.home.jog 0
@18:35:40.23 <Lysis1_home> Lysis1.home.jog 0
@18:35:40.25 <PinPort_home> PinPort.home.jog 0
@18:35:40.28 <Lysis2_home> Lysis2.home.jog 0
@18:35:40.30 <Air_home> Air.home.jog 0
@18:35:40.62 <Archive_home> Archive.in :homing
@18:35:40.86 <Toroid_home> Toroid.in :homing
@18:35:40.89 <Plunge_home> Plunge.in :homing
@18:35:40.94 <Archive_home> Archive.jog 150
@18:35:40.98 <Lysis1_home> Lysis1.in :homing
@18:35:41.02 <PinPort_home> PinPort.in :homing
@18:35:41.20 <Toroid_home> Toroid.jog 624
@18:35:41.26 <Plunge_home> Plunge.jog 1578
@18:35:41.30 <PinPort_home> PinPort.jog -107
@18:35:41.32 <Lysis1_home> Lysis1.jog 1416
@18:35:41.56 <Air_home> Air.in :homing
@18:35:41.58 <Lysis2_home> Lysis2.in :homing
@18:35:41.68 Lysis2.jog 1428
@18:35:41.70 <Air_home> Air.jog 152
@18:35:41.82 <PinPort_home> PinPort.setRawPosition! 0
@18:35:41.83 <Archive_home> Archive.setRawPosition! 50
@18:35:41.90 <PinPort_home> PinPort.seek :home
@18:35:41.94 <Archive_home> Archive.seek :home
@18:35:42.08 <PinPort_home> PinPort.in :default
@18:35:42.17 <Air_home> Air.setRawPosition! 50
@18:35:42.22 Air.seek :home
@18:35:42.31 <Archive_home> Archive.in :default
@18:35:42.50 <Air_home> Air.in :default
@18:35:42.60 <Plunge_home> Plunge.setRawPosition! 50
@18:35:42.62 <Lysis1_home> Lysis1.setRawPosition! 50
@18:35:42.70 Lysis1.seek :home
@18:35:42.73 <Plunge_home> Plunge.seek :home
@18:35:42.88 <Lysis2_home> Lysis2.setRawPosition! 50
@18:35:42.92 Lysis2.seek :home
@18:35:43.02 <Lysis1_home> Lysis1.in :default
@18:35:43.10 <Plunge_home> Plunge.in :default
@18:35:43.20 <Lysis2_home> Lysis2.in :default
@18:35:43.45 <Toroid_home> Toroid.setRawPosition! 0
@18:35:43.50 Toroid.seek :home
@18:35:43.58 Toroid.in :default
@18:35:43.71 <LRAUV> Toroid.seek :clear
@18:35:45.34 Carousel.in :default
@18:35:45.40 Carousel.home.jog 0
@18:35:45.89 Carousel.in :default
@18:35:45.95 Carousel.seek 16
@18:35:46.11 Selecting Cartridge 11
@18:35:46.36 Carousel.seek 11
@18:35:47.65 Lysis1.in :fast
@18:35:47.71 Lysis1.seek :locked
@18:35:50.31 <Plunge> Plunge.seek :locked
@18:35:50.50 <Air> Air.in :fast
@18:35:50.56 Air.seek :locked
@18:35:53.17 <LRAUV> Powering cartridge bus
@18:35:55.29 Gate.setIR 1750,5000,0,6
@18:35:55.42 Gate.setIR 0,5000,0,6
@18:35:55.49 PuckHeater.configure :default
@18:35:55.53 Gate.power :heat,:ON,limit to 5000mA
@18:35:56.20 Mounted lysis Cartridge board#Q05042
@18:35:56.23 Using default puck heat loss curve for HeatingAccelInCarousel
@18:36:15.29 -> Cmd.startFiltering
@18:36:15.73 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@18:36:15.75 SamplePump.setPosition! 0ml
@18:36:15.79 Bypass.open
@18:36:17.30 Intake.open
@18:36:18.09 Sea 4.5m deep (21.4psia)
@18:36:18.11 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@18:36:20.69 Vacuum tare is 51% of full scale
@18:36:20.82 Pressure.in :default
@18:36:20.88 Pressure.hold
@18:36:21.05 SamplePump.in :prime
@18:36:21.11 SamplePump.seek 10ml,1:21
@18:36:30.50 Pressure.coast
@18:36:30.55 SamplePump.setPosition! 0ml
@18:36:30.60 Exhaust.open
@18:36:32.03 <closeBypass> Bypass.close
@18:36:33.61 <FILTERING> Pressure.in :default
@18:36:33.67 Pressure.hold
@18:36:33.78 Sea 3.8m deep (20.3psia)
@18:36:33.91 SamplePump.in :prime
@18:36:33.98 SamplePump.seek 100ml,8:51
@18:38:43.68 Pressure.coast
@18:38:43.74 Bypass.open
@18:38:44.49 Intake.close
@18:38:45.33 Toroid.in :hold
@18:38:45.39 Toroid.seek :crack
@18:38:46.95 Toroid.seek :cartridge
@18:38:48.04 Toroid.in :default
@18:38:48.10 Toroid.seek :clear
@18:38:50.06 Pressurizing cartridge to 26psi above 19.9psia
@18:38:50.07 SamplePump.setPosition! 0ml
@18:38:50.11 Intake.open
@18:38:50.84 Bypass.close
@18:38:51.61 Exhaust.close
@18:38:52.80 Pressure.in :default
@18:38:52.86 Pressure.hold
@18:38:53.03 SamplePump.in :charge
@18:38:53.10 SamplePump.seek 2.3ml,42 seconds
@18:39:02.80 Pressure.coast
@18:39:02.85 Intake.close
@18:39:03.67 Pressurized cartridge with 0.418ml to 27.2psi
@18:39:03.68 Isolated @42.2psia (22.3psi)
@18:39:03.69 Verifying cartridge holds at least 0.75..0.85 of 42.2psia
@18:39:03.72 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@18:39:08.34 Sampling 1000.0ml
@18:39:08.36 SamplePump.setPosition! 0ml
@18:39:08.41 Exhaust.open
@18:39:09.10 Intake.open
@18:39:09.97 Pressure.in :default
@18:39:10.03 Pressure.hold
@18:39:10.14 Sea 5.1m deep (22.2psia)
@18:39:10.27 SamplePump.in :sample
@18:39:10.34 SamplePump.seek 1000ml,1:23:53
@18:39:13.00 <sampling> Cartridge 11 filtering 1/1000ml at 0.35ml/s with 25.1psi vacuum
@18:40:12.99 Cartridge 11 filtering 19/1000ml at 0.32ml/s with 20.6psi vacuum 5.6m deep (23.0psia)
@18:41:13.00 Cartridge 11 filtering 37/1000ml at 0.32ml/s with 25.2psi vacuum 2.5m deep (18.5psia)
@18:42:12.99 Cartridge 11 filtering 55/1000ml at 0.29ml/s with 21.9psi vacuum 4.9m deep (21.9psia)
@18:43:13.00 Cartridge 11 filtering 72/1000ml at 0.24ml/s with 25.5psi vacuum
@18:44:12.99 Cartridge 11 filtering 89/1000ml at 0.30ml/s with 20.5psi vacuum 5.3m deep (22.5psia)
@18:45:13.00 Cartridge 11 filtering 105/1000ml at 0.25ml/s with 21.6psi vacuum 2.3m deep (18.1psia)
@18:46:12.99 Cartridge 11 filtering 122/1000ml at 0.27ml/s with 27.5psi vacuum 3.6m deep (20.0psia)
@18:47:13.00 Cartridge 11 filtering 138/1000ml at 0.30ml/s with 24.7psi vacuum 4.2m deep (21.0psia)
@18:48:12.99 Cartridge 11 filtering 154/1000ml at 0.28ml/s with 20.7psi vacuum 3.2m deep (19.5psia)
@18:49:13.00 Cartridge 11 filtering 169/1000ml at 0.29ml/s with 23.1psi vacuum 5.4m deep (22.6psia)
@18:50:12.99 Cartridge 11 filtering 184/1000ml at 0.27ml/s with 27.1psi vacuum
@18:51:13.00 Cartridge 11 filtering 199/1000ml at 0.25ml/s with 21.5psi vacuum 4.9m deep (22.0psia)
@18:52:13.00 Cartridge 11 filtering 214/1000ml at 0.20ml/s with 23.4psi vacuum 1.7m deep (17.3psia)
@18:53:13.00 Cartridge 11 filtering 229/1000ml at 0.23ml/s with 27.0psi vacuum 4.9m deep (22.0psia)
@18:54:13.00 Cartridge 11 filtering 243/1000ml at 0.24ml/s with 21.9psi vacuum 2.8m deep (18.8psia)
@18:55:13.00 Cartridge 11 filtering 257/1000ml at 0.23ml/s with 26.8psi vacuum 3.5m deep (19.8psia)
@18:56:13.00 Cartridge 11 filtering 271/1000ml at 0.20ml/s with 25.0psi vacuum 3.1m deep (19.3psia)
@18:57:13.00 Cartridge 11 filtering 285/1000ml at 0.23ml/s with 21.5psi vacuum 3.4m deep (19.6psia)
@18:58:13.00 Cartridge 11 filtering 299/1000ml at 0.21ml/s with 21.8psi vacuum 5.3m deep (22.5psia)
@18:59:13.00 Cartridge 11 filtering 312/1000ml at 0.23ml/s with 21.0psi vacuum 2.4m deep (18.3psia)
@19:00:13.00 Cartridge 11 filtering 325/1000ml at 0.22ml/s with 23.8psi vacuum 4.3m deep (21.1psia)
@19:01:13.00 Cartridge 11 filtering 339/1000ml at 0.23ml/s with 25.3psi vacuum 2.4m deep (18.2psia)
@19:02:13.00 Cartridge 11 filtering 352/1000ml at 0.20ml/s with 27.4psi vacuum 4.0m deep (20.6psia)
@19:03:13.00 Cartridge 11 filtering 365/1000ml at 0.20ml/s with 24.7psi vacuum 2.1m deep (17.9psia)
@19:04:13.00 Cartridge 11 filtering 378/1000ml at 0.20ml/s with 28.2psi vacuum 3.3m deep (19.6psia)
@19:05:13.00 Cartridge 11 filtering 391/1000ml at 0.23ml/s with 27.1psi vacuum 2.7m deep (18.7psia)
@19:06:13.00 Cartridge 11 filtering 404/1000ml at 0.20ml/s with 26.3psi vacuum 2.6m deep (18.5psia)
@19:07:13.00 Cartridge 11 filtering 417/1000ml at 0.20ml/s with 23.1psi vacuum 3.1m deep (19.2psia)
@19:08:13.00 Cartridge 11 filtering 429/1000ml at 0.20ml/s with 29.3psi vacuum
@19:09:13.00 Cartridge 11 filtering 442/1000ml at 0.21ml/s with 24.6psi vacuum 4.7m deep (21.6psia)
@19:10:13.00 Cartridge 11 filtering 454/1000ml at 0.20ml/s with 24.5psi vacuum
@19:11:13.00 Cartridge 11 filtering 467/1000ml at 0.20ml/s with 26.2psi vacuum 3.9m deep (20.4psia)
@19:12:13.00 Cartridge 11 filtering 479/1000ml at 0.20ml/s with 28.3psi vacuum 1.7m deep (17.2psia)
@19:13:13.00 Cartridge 11 filtering 491/1000ml at 0.20ml/s with 25.3psi vacuum 3.8m deep (20.4psia)
@19:14:13.00 Cartridge 11 filtering 504/1000ml at 0.22ml/s with 24.9psi vacuum 3.0m deep (19.2psia)
@19:14:39.06 <FILTERING> Flow averaged 0.24ml/s over 35:29
@19:14:39.09 Waiting up to 11 seconds for vacuum to reduce from 27.0 to 18.0psi
@19:14:39.66 Relieved excess vacuum in .6 seconds
@19:14:39.68 SamplePump.seek 1000ml,41:27
@19:14:44.35 Flow estimate reduced to 0.19ml/s
@19:14:44.37 <closeIntake> Intake.close
@19:14:44.41 <closeExhaust> Exhaust.close
@19:14:44.60 <FILTERING> Toroid.in :hold
@19:14:44.67 Toroid.seek :bypass
@19:14:46.30 Toroid.in :default
@19:14:46.36 Toroid.seek :clear
@19:14:47.97 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@19:14:47.98 Pressure.coast
@19:14:48.16 Sampled  509.9ml
@19:14:50.30 <LRAUV> -> Cmd.startProcessing
@19:14:51.73 <PROCESSING> SPR.command \"read_sequences\"
@19:14:52.53 SPR.command \"stats_on\"
@19:14:52.57 SPR.command \"start 0 1\"
@19:14:54.58 <SPRlogger> Begun log /var/log/esp/spr21apr2419h.spr
@19:14:55.39 <PROCESSING> Handoff.select :waste
@19:15:02.07 Syringe tare is 20% of full scale
@19:15:02.09 PinPort.in :locking
@19:15:02.16 PinPort.seek :locked
@19:15:02.64 <posArchive> Archive.in :fast
@19:15:02.68 <posLyS2> Lysis2.in :fast
@19:15:02.74 <PROCESSING> Lysis1.seek 1350uL
@19:15:02.84 <posArchive> Archive.seek :full
@19:15:02.89 <posLyS2> Lysis2.seek :full
@19:15:04.99 <PROCESSING> Plunge.seek :reagent
@19:15:05.89 Evacuating puck with filter bubble point of 50psi
@19:15:05.91 PinPort.seek :locked
@19:15:05.99 Handoff.select :waste
@19:15:12.57 Air syringe pumping up to 1 stroke to reach 8psi
@19:15:12.58 Air stroke #1 of 1
@19:15:12.67 Air.in :pressurize
@19:15:12.73 Air.seek :pressurized
@19:15:23.99 0.1psi after pressurize
@19:15:24.00 Air.in :compress
@19:15:24.07 Air.seek :evac
@19:15:28.58 0.5psi after compress
@19:15:41.16 0.0psi after compress (stable)
@19:15:41.17 Air.in :default
@19:15:41.24 Air.hold
@19:15:41.43 at -0.0psi Opened to waste
@19:15:41.44 Handoff.select :blocked
@19:15:41.57 Archive syringe pumping up to 5 strokes to reach 50psi
@19:15:41.58 Archive stroke #1 of 5
@19:15:41.65 Archive.in :pressurize
@19:15:41.72 Archive.seek :pressurized
@19:15:54.46 4.3psi after pressurize
@19:15:54.50 Archive.in :compress
@19:15:54.57 Archive.seek :empty
@19:16:10.47 29.5psi after compress
@19:16:40.49 Reached 50.0 of 50.0psi after pumping 1730uL (1.00 strokes)
@19:16:40.50 Archive.in :default
@19:16:40.56 Archive.hold
@19:16:40.63 Handoff.select :waste
@19:16:50.82 delay 1:00
@19:17:50.84 Handoff.select :blocked
@19:18:13.56 Handoff.select :waste
@19:18:13.69 2.3psi at evacEnd
@19:18:13.70 Handoff.select :blocked
@19:18:13.84 Archive.in :fast
@19:18:13.90 Archive.seek :topVent
@19:18:27.62 Stable @2.9psi after Handoff blocked
@19:18:27.64 Archive syringe pumping up to 5 strokes to reach 33psi
@19:18:27.66 Archive stroke #1 of 5
@19:18:27.77 Archive.in :pressurize
@19:18:27.83 Archive.seek :pressurized
@19:18:40.57 4.3psi after pressurize
@19:18:40.58 Archive.in :compress
@19:18:40.66 Archive.seek :empty
@19:18:56.55 6.6psi after compress
@19:19:33.72 Reached 33.0 of 32.9psi after pumping 1730uL (1.00 strokes)
@19:19:33.73 Archive.in :default
@19:19:33.80 Archive.hold
@19:19:33.87 Handoff.select :waste
@19:19:45.07 delay 1:00
@19:20:45.09 Handoff.select :blocked
@19:21:07.78 Handoff.select :waste
@19:21:07.90 2.3psi at evacEnd
@19:21:07.92 Air.seek :fullVent
@19:21:09.37 Archive.in :fast
@19:21:09.43 Archive.seek :topVent
@19:21:16.60 Seawater evacuated, Presure above/below filter released. Lysis begins
@19:21:16.69 Lysis1.in :slow
@19:21:16.75 Lysis1.seek 1050uL
@19:21:46.79 delay 5 seconds
@19:21:51.81 Handoff.select :blocked
@19:21:51.97 Lysis1.seek 450uL
@19:22:52.00 delay 3 seconds
@19:22:55.07 Air.in to mix into 1.37806 parts Lysis1
@19:22:55.59 <Airmixer> Air.seek :evac
@19:22:55.63 <PROCESSING> Lysis1.seek 300uL
@19:23:10.76 Lysis1.seek :empty
@19:23:40.87 Lysis buffer delivered @40.4psi
@19:23:40.88 Polling Can every 2:00
@19:23:41.43 PuckHeater.configure :default #ambient 12.0C
@19:23:41.48 PuckHeater.seek 80.2C
@19:24:48.82 PuckHeater.hold 2:00,80.2C
@19:26:49.01 Polling Can every 2:00
@19:26:49.56 PuckHeater.configure :default #ambient 12.1C
@19:26:49.61 PuckHeater.hold 30 seconds,79.2C
@19:27:19.81 Polling Can every 2:00
@19:27:20.35 PuckHeater.configure :default #ambient 12.1C
@19:27:20.39 PuckHeater.hold 30 seconds,78.6C
@19:27:50.61 Polling Can every 2:00
@19:27:51.16 PuckHeater.configure :default #ambient 12.0C
@19:27:51.21 PuckHeater.hold 10:00,78.1C
@19:37:51.41 Lysis complete. Syringe pressure @50.3psi
@19:37:51.42 Handoff.select :blocked
@19:37:51.47 Archive syringe pumping up to 3 strokes to reach 40psi
@19:37:51.48 Archive stroke #1 of 3
@19:37:51.57 Archive.in :pressurize
@19:37:51.63 Archive.seek :pressurized
@19:37:51.80 Reached 50.4 of 40.0psi after pumping 0uL (0.00 strokes)
@19:37:51.81 Archive.in :default
@19:37:51.88 Archive.hold
@19:37:51.95 delay 2 seconds
@19:37:53.97 Archive.in :fast
@19:37:54.04 Archive.seek :full
@19:37:54.21 Lysis1.seek 138uL
@19:38:08.09 delay 1 second
@19:38:09.14 <fillArchive> Archive.seek :full
@19:38:09.16 <PROCESSING> Lysis1.seek :empty
@19:38:23.16 Initial air pressure @50.5psi
@19:38:23.24 Lysis1.in :extract
@19:38:23.34 Lysis1.seek 600uL,nil
@19:38:25.08 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@19:38:25.09 Archive stroke #1 of 6
@19:38:25.19 Archive.in :pressurize
@19:38:25.26 Archive.seek :pressurized
@19:38:38.03 15.8psi after pressurize
@19:38:38.04 Archive.in :compress
@19:38:38.11 Archive.seek :empty
@19:38:54.00 15.7psi after compress
@19:39:06.58 15.8psi after compress (stable)
@19:39:11.71 Recovering 4.0uL/s
@19:39:16.78 Recovering 2.2uL/s
@19:39:21.84 Recovering 2.6uL/s
@19:39:26.96 Archive.in :gulp
@19:39:27.02 Archive.seek :topVent
@19:39:34.23 15.2psi after gulp
@19:39:46.81 15.9psi after gulp (stable)
@19:39:46.82 Archive stroke #2 of 6
@19:39:46.91 Archive.in :pressurize
@19:39:46.98 Archive.seek :pressurized
@19:39:59.77 15.7psi after pressurize
@19:39:59.79 Archive.in :compress
@19:39:59.86 Archive.seek :empty
@19:40:09.78 Archive.in :default
@19:40:09.83 Archive.hold
@19:40:10.07 <PROCESSING> Initial 600uL lysate @16.0psi
@19:40:10.08 Handoff.select :blocked
@19:40:10.19 Initial air pressure @16.0psi
@19:40:10.24 Lysis1.seek 825uL,nil
@19:40:11.46 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@19:40:11.47 Archive stroke #1 of 6
@19:40:11.56 Archive.in :compress
@19:40:11.63 Archive.seek :empty
@19:40:17.63 15.7psi after compress
@19:40:24.93 Archive.in :default
@19:40:24.98 Archive.hold
@19:40:25.16 <PROCESSING> Recovered 825uL lysate @16.0psi
@19:40:25.18 Air.in :halfSpeed
@19:40:25.26 Air.seek :fullVent
@19:40:27.51 Puck Top Vented, bottom @3.1psi
@19:40:27.52 Handoff.select :waste
@19:40:27.64 delay 5 seconds
@19:40:32.70 Handoff open to waste, bottom @0.0psi
@19:40:32.72 Air.in :default
@19:40:32.79 Air.seek :evac
@19:40:34.25 Lysis2.in :DAmix
@19:40:34.35 Lysis1.in :DAmix
@19:40:34.84 <Lysis2mixer> Lysis2.seek 1480uL
@19:40:34.87 <PROCESSING> Lysis1.seek 575uL
@19:41:09.97 delay 5 seconds
@19:41:14.99 Handoff.select SPR
@19:41:15.10 SPR.command \"start 2 14\"
@19:41:16.45 delay 10 seconds
@19:41:26.47 SPR.command \"start 1 0\"
@19:41:29.22 Waiting up to 4:00 for SPR to enter state 1
@19:43:31.60 SPR.command \"hold_on\"
@19:43:33.01 SPR Dilution 1 of 1 -- Running Experiment 1
@19:43:33.43 <Lysis2mixer> Lysis2.seek 1180uL
@19:43:33.50 <PROCESSING> Lysis1.seek 275uL
@19:44:15.52 volL1 = 300.0 and volL2 = 300.0
@19:44:15.53 delay 15 seconds
@19:44:30.55 SPR.command \"hold_off\"
@19:44:33.67 Waiting up to 38:00 for SPR to enter state 7
@20:11:17.21 SPR Analysis has Completed
@20:11:17.23 Handoff.select :waste
@20:11:18.14 <SPRlogger> Ended log /var/log/esp/spr21apr2419h.spr
@20:11:18.15 <PROCESSING> SPR.command \"stats_off\"
@20:11:19.61 <LRAUV> -> Cmd.stop
@20:11:19.70 <closeHandoff> Handoff.select :close
@20:11:19.83 <LRAUV> Gate.power :heat,:OFF
@20:11:19.85 Gate.power :cartridge,:OFF
@20:11:19.93 Carousel.seek 11
@20:11:20.10 Plunge.seek :locked
@20:11:20.99 PinPort.in :unlocking
@20:11:21.05 PinPort.seek :clear
@20:11:21.59 <Archive> Archive.in :unlocking
@20:11:21.72 Archive.seek :clear
@20:11:21.79 <Air> Air.in :unlocking
@20:11:21.91 Air.seek :clear
@20:11:21.99 <Lysis1> Lysis1.in :unlocking
@20:11:22.11 Lysis1.seek :clear
@20:11:22.14 <Lysis2> Lysis2.in :unlocking
@20:11:22.27 Lysis2.seek :clear
@20:11:25.78 <LRAUV> Archive.coast
@20:11:25.83 Air.coast
@20:11:25.87 Lysis1.coast
@20:11:25.92 Lysis2.coast
@20:11:25.97 Plunge.coast
@20:11:26.01 SamplePump.coast
@20:11:26.06 Toroid.coast
@20:11:26.11 Carousel.coast
@20:11:26.16 PinPort.coast
@20:11:26.33 Gate.power :motor,:OFF
@20:11:26.35 Gate.power :core,:OFF
@20:11:26.37 Gate.power :analytic1,:OFF
@20:11:26.39 Gate.power :analytic2,:OFF
@20:11:26.41 Gate.power :analytic3,:OFF
@20:11:26.43 Gate.power :main,:OFF
@20:11:26.45 Safely stopped and ready to power off
##-- file closed 2021-04-25T05:18:01.466Z

##-- file opened 2021-04-25T05:18:01.467Z
@22:18:01.74 -> showStatus
@22:18:03.76 -> Cmd.loadCartridge -6
@22:18:03.91 Gate.power :main,:ON,limit to 5000mA
@22:18:03.92 Gate.power :analytic1,:ON,limit to 5000mA
@22:18:03.95 Gate.power :analytic2,:ON,limit to 5000mA
@22:18:03.97 Gate.power :analytic3,:ON,limit to 5000mA
@22:18:04.45 Powering core bus
@22:18:06.39 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@22:18:06.69 <LRAUV> Archive.configure :default
@22:18:06.82 Air.configure :default
@22:18:06.92 Lysis1.configure :default
@22:18:07.03 Lysis2.configure :default
@22:18:07.15 Plunge.configure :default
@22:18:07.27 SamplePump.configure :default
@22:18:07.42 ExtraPump.configure :default
@22:18:07.53 Toroid.configure :default
@22:18:07.66 Carousel.configure :default
@22:18:07.78 Pressure.configure :default
@22:18:07.89 PinPort.configure :default
@22:18:07.96 All elf controllers running firmware version 4.741
@22:18:07.99 Gate.power :motor,:ON,limit to 5000mA
@22:18:08.14 <Archive_home> Archive.in :default
@22:18:08.43 Archive.home.jog 0
@22:18:08.62 <Toroid_home> Toroid.in :default
@22:18:08.68 <Plunge_home> Plunge.in :default
@22:18:08.72 <Lysis1_home> Lysis1.in :default
@22:18:08.75 <Air_home> Air.in :default
@22:18:08.81 <PinPort_home> PinPort.in :default
@22:18:08.92 <Lysis2_home> Lysis2.in :default
@22:18:09.03 <Toroid_home> Toroid.home.jog 0
@22:18:09.09 <Plunge_home> Plunge.home.jog 0
@22:18:09.12 <Lysis1_home> Lysis1.home.jog 0
@22:18:09.16 <Air_home> Air.home.jog 0
@22:18:09.19 <PinPort_home> PinPort.home.jog 0
@22:18:09.24 <Lysis2_home> Lysis2.home.jog 0
@22:18:09.59 <Archive_home> Archive.in :homing
@22:18:09.88 <Toroid_home> Toroid.in :homing
@22:18:09.91 <Plunge_home> Plunge.in :homing
@22:18:09.94 <Air_home> Air.in :homing
@22:18:09.99 <PinPort_home> PinPort.in :homing
@22:18:10.01 <Lysis2_home> Lysis2.in :homing
@22:18:10.05 <Archive_home> Archive.jog 152
@22:18:10.10 <Lysis1_home> Lysis1.in :homing
@22:18:10.28 <Toroid_home> Toroid.jog 619
@22:18:10.32 <Plunge_home> Plunge.jog 1578
@22:18:10.35 <Air_home> Air.jog 170
@22:18:10.38 <Lysis2_home> Lysis2.jog 150
@22:18:10.41 <PinPort_home> PinPort.jog -107
@22:18:10.45 <Lysis1_home> Lysis1.jog 1413
@22:18:10.69 <Archive_home> Archive.setRawPosition! 50
@22:18:10.74 Archive.seek :home
@22:18:10.82 <PinPort_home> PinPort.setRawPosition! 0
@22:18:10.87 PinPort.seek :home
@22:18:10.98 <Lysis2_home> Lysis2.setRawPosition! 50
@22:18:11.01 <PinPort_home> PinPort.in :default
@22:18:11.02 <Air_home> Air.setRawPosition! 50
@22:18:11.12 <Lysis2_home> Lysis2.seek :home
@22:18:11.16 <Air_home> Air.seek :home
@22:18:11.28 <Archive_home> Archive.in :default
@22:18:11.47 <Lysis2_home> Lysis2.in :default
@22:18:11.54 <Air_home> Air.in :default
@22:18:11.71 <Plunge_home> Plunge.setRawPosition! 50
@22:18:11.77 Plunge.seek :home
@22:18:11.85 <Lysis1_home> Lysis1.setRawPosition! 50
@22:18:11.89 Lysis1.seek :home
@22:18:12.04 <Plunge_home> Plunge.in :default
@22:18:12.17 <Lysis1_home> Lysis1.in :default
@22:18:12.52 <Toroid_home> Toroid.setRawPosition! 0
@22:18:12.57 Toroid.seek :home
@22:18:12.66 Toroid.in :default
@22:18:12.78 <LRAUV> Toroid.seek :clear
@22:18:14.40 Carousel.in :default
@22:18:14.46 Carousel.home.jog 0
@22:18:14.96 Carousel.in :default
@22:18:15.02 Carousel.seek 11
@22:18:15.15 Selecting Cartridge 14
@22:18:15.39 Carousel.seek 14
@22:18:16.28 Lysis1.in :fast
@22:18:16.35 Lysis1.seek :locked
@22:18:18.94 <Plunge> Plunge.seek :locked
@22:18:19.06 <Air> Air.in :fast
@22:18:19.12 Air.seek :locked
@22:18:21.73 <LRAUV> Powering cartridge bus
@22:18:23.85 Gate.setIR 1750,5000,0,6
@22:18:23.98 Gate.setIR 0,5000,0,6
@22:18:24.03 Gate.power :cartridge,:OFF
@22:18:24.56 Mounted archive Cartridge
@22:18:43.50 -> Cmd.startFiltering
@22:18:43.97 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@22:18:43.98 SamplePump.setPosition! 0ml
@22:18:44.03 Bypass.open
@22:18:45.51 Intake.open
@22:18:46.30 Sea 6.6m deep (24.5psia)
@22:18:46.32 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@22:18:48.91 Vacuum tare is 51% of full scale
@22:18:49.04 Pressure.in :default
@22:18:49.09 Pressure.hold
@22:18:49.27 SamplePump.in :prime
@22:18:49.34 SamplePump.seek 10ml,1:21
@22:18:58.73 Pressure.coast
@22:18:58.78 SamplePump.setPosition! 0ml
@22:18:58.83 Exhaust.open
@22:19:00.27 <closeBypass> Bypass.close
@22:19:01.81 <FILTERING> Pressure.in :default
@22:19:01.87 Pressure.hold
@22:19:01.98 Sea 6.1m deep (23.8psia)
@22:19:02.11 SamplePump.in :prime
@22:19:02.17 SamplePump.seek 100ml,8:51
@22:21:11.92 Pressure.coast
@22:21:11.98 Bypass.open
@22:21:12.71 Intake.close
@22:21:13.56 Toroid.in :hold
@22:21:13.62 Toroid.seek :crack
@22:21:15.17 Toroid.seek :cartridge
@22:21:16.27 Toroid.in :default
@22:21:16.33 Toroid.seek :clear
@22:21:18.30 Pressurizing cartridge to 26psi above 24.9psia
@22:21:18.31 SamplePump.setPosition! 0ml
@22:21:18.36 Intake.open
@22:21:19.08 Bypass.close
@22:21:19.84 Exhaust.close
@22:21:20.68 Pressure.in :default
@22:21:21.13 Pressure.hold
@22:21:21.30 SamplePump.in :charge
@22:21:21.37 SamplePump.seek 2.3ml,42 seconds
@22:21:27.04 Pressure.coast
@22:21:27.09 Intake.close
@22:21:27.90 Pressurized cartridge with 0.141ml to 26.0psi
@22:21:27.91 Isolated @45.1psia (20.3psi)
@22:21:27.93 Verifying cartridge holds at least 0.75..0.85 of 45.1psia
@22:21:27.96 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@22:21:32.58 Sampling 1000.0ml
@22:21:32.60 SamplePump.setPosition! 0ml
@22:21:32.65 Exhaust.open
@22:21:33.35 Intake.open
@22:21:34.22 Pressure.in :default
@22:21:34.27 Pressure.hold
@22:21:34.40 Sea 6.1m deep (23.8psia)
@22:21:34.53 SamplePump.in :sample
@22:21:34.60 SamplePump.seek 1000ml,1:23:53
@22:21:37.26 <sampling> Cartridge 14 filtering 1/1000ml at 0.36ml/s with 25.8psi vacuum 4.3m deep (21.1psia)
@22:22:37.25 Cartridge 14 filtering 20/1000ml at 0.30ml/s with 20.6psi vacuum 2.7m deep (18.7psia)
@22:23:37.26 Cartridge 14 filtering 39/1000ml at 0.26ml/s with 25.8psi vacuum 2.6m deep (18.5psia)
@22:24:37.25 Cartridge 14 filtering 58/1000ml at 0.27ml/s with 27.8psi vacuum
@22:25:37.26 Cartridge 14 filtering 77/1000ml at 0.26ml/s with 21.4psi vacuum 4.1m deep (20.8psia)
@22:26:37.25 Cartridge 14 filtering 95/1000ml at 0.34ml/s with 22.7psi vacuum 2.3m deep (18.1psia)
@22:27:37.26 Cartridge 14 filtering 113/1000ml at 0.25ml/s with 22.9psi vacuum 4.0m deep (20.6psia)
@22:28:37.25 Cartridge 14 filtering 131/1000ml at 0.25ml/s with 27.3psi vacuum
@22:29:37.26 Cartridge 14 filtering 149/1000ml at 0.25ml/s with 21.4psi vacuum 3.4m deep (19.7psia)
@22:30:37.25 Cartridge 14 filtering 166/1000ml at 0.32ml/s with 25.9psi vacuum 4.2m deep (20.9psia)
@22:31:37.26 Cartridge 14 filtering 183/1000ml at 0.32ml/s with 25.0psi vacuum 3.7m deep (20.2psia)
@22:32:37.25 Cartridge 14 filtering 200/1000ml at 0.24ml/s with 22.5psi vacuum 4.6m deep (21.4psia)
@22:33:37.26 Cartridge 14 filtering 218/1000ml at 0.23ml/s with 22.6psi vacuum 1.7m deep (17.2psia)
@22:34:37.25 Cartridge 14 filtering 234/1000ml at 0.30ml/s with 22.8psi vacuum 5.7m deep (23.2psia)
@22:35:37.26 Cartridge 14 filtering 251/1000ml at 0.29ml/s with 23.7psi vacuum 2.9m deep (19.0psia)
@22:36:37.26 Cartridge 14 filtering 268/1000ml at 0.31ml/s with 23.7psi vacuum 5.4m deep (22.7psia)
@22:37:37.26 Cartridge 14 filtering 284/1000ml at 0.31ml/s with 27.6psi vacuum 2.8m deep (18.9psia)
@22:38:37.25 Cartridge 14 filtering 300/1000ml at 0.25ml/s with 19.5psi vacuum 2.7m deep (18.7psia)
@22:39:37.26 Cartridge 14 filtering 316/1000ml at 0.28ml/s with 23.4psi vacuum 4.5m deep (21.3psia)
@22:40:37.26 Cartridge 14 filtering 332/1000ml at 0.22ml/s with 22.1psi vacuum 1.9m deep (17.5psia)
@22:41:37.26 Cartridge 14 filtering 348/1000ml at 0.25ml/s with 19.7psi vacuum 4.8m deep (21.8psia)
@22:42:37.26 Cartridge 14 filtering 364/1000ml at 0.23ml/s with 21.4psi vacuum 2.0m deep (17.7psia)
@22:43:37.26 Cartridge 14 filtering 380/1000ml at 0.28ml/s with 23.3psi vacuum 4.3m deep (21.1psia)
@22:44:37.26 Cartridge 14 filtering 395/1000ml at 0.28ml/s with 23.8psi vacuum 3.9m deep (20.4psia)
@22:45:37.26 Cartridge 14 filtering 410/1000ml at 0.24ml/s with 26.0psi vacuum 3.2m deep (19.4psia)
@22:46:37.26 Cartridge 14 filtering 426/1000ml at 0.21ml/s with 27.8psi vacuum 2.8m deep (18.8psia)
@22:47:37.26 Cartridge 14 filtering 441/1000ml at 0.28ml/s with 25.4psi vacuum 3.5m deep (19.9psia)
@22:48:37.26 Cartridge 14 filtering 456/1000ml at 0.20ml/s with 23.0psi vacuum 3.9m deep (20.5psia)
@22:49:37.26 Cartridge 14 filtering 471/1000ml at 0.20ml/s with 26.7psi vacuum
@22:50:37.26 Cartridge 14 filtering 485/1000ml at 0.26ml/s with 23.7psi vacuum 4.5m deep (21.4psia)
@22:51:37.26 Cartridge 14 filtering 500/1000ml at 0.25ml/s with 26.5psi vacuum
@22:52:37.26 Cartridge 14 filtering 514/1000ml at 0.22ml/s with 21.8psi vacuum 4.5m deep (21.4psia)
@22:53:37.26 Cartridge 14 filtering 529/1000ml at 0.25ml/s with 21.9psi vacuum 1.8m deep (17.3psia)
@22:54:37.26 Cartridge 14 filtering 543/1000ml at 0.25ml/s with 25.3psi vacuum 3.9m deep (20.4psia)
@22:55:37.26 Cartridge 14 filtering 557/1000ml at 0.21ml/s with 22.6psi vacuum 4.4m deep (21.2psia)
@22:56:37.26 Cartridge 14 filtering 571/1000ml at 0.25ml/s with 24.7psi vacuum 3.0m deep (19.1psia)
@22:57:37.26 Cartridge 14 filtering 585/1000ml at 0.24ml/s with 23.4psi vacuum 4.9m deep (21.9psia)
@22:58:37.27 Cartridge 14 filtering 599/1000ml at 0.20ml/s with 24.0psi vacuum
@22:59:37.26 Cartridge 14 filtering 613/1000ml at 0.24ml/s with 22.7psi vacuum 6.5m deep (24.3psia)
@23:00:37.26 Cartridge 14 filtering 627/1000ml at 0.24ml/s with 25.4psi vacuum
@23:01:37.26 Cartridge 14 filtering 640/1000ml at 0.20ml/s with 22.9psi vacuum 4.6m deep (21.6psia)
@23:02:37.26 Cartridge 14 filtering 654/1000ml at 0.23ml/s with 25.9psi vacuum 2.8m deep (18.9psia)
@23:03:37.26 Cartridge 14 filtering 667/1000ml at 0.22ml/s with 27.9psi vacuum 2.3m deep (18.1psia)
@23:04:37.26 Cartridge 14 filtering 681/1000ml at 0.20ml/s with 27.3psi vacuum 4.2m deep (20.9psia)
@23:05:37.26 Cartridge 14 filtering 694/1000ml at 0.24ml/s with 26.2psi vacuum 2.7m deep (18.7psia)
@23:06:37.26 Cartridge 14 filtering 707/1000ml at 0.20ml/s with 22.8psi vacuum 4.4m deep (21.2psia)
@23:07:37.26 Cartridge 14 filtering 720/1000ml at 0.23ml/s with 24.4psi vacuum 2.4m deep (18.3psia)
@23:08:37.26 Cartridge 14 filtering 733/1000ml at 0.22ml/s with 24.4psi vacuum 5.0m deep (22.2psia)
@23:09:37.26 Cartridge 14 filtering 746/1000ml at 0.20ml/s with 22.6psi vacuum
@23:10:37.26 Cartridge 14 filtering 759/1000ml at 0.24ml/s with 26.0psi vacuum 4.2m deep (20.9psia)
@23:11:37.26 Cartridge 14 filtering 772/1000ml at 0.20ml/s with 23.4psi vacuum 4.6m deep (21.5psia)
@23:12:37.26 Cartridge 14 filtering 785/1000ml at 0.20ml/s with 28.1psi vacuum 2.8m deep (18.8psia)
@23:13:37.26 Cartridge 14 filtering 798/1000ml at 0.22ml/s with 26.2psi vacuum 4.3m deep (21.1psia)
@23:14:37.26 Cartridge 14 filtering 810/1000ml at 0.21ml/s with 25.0psi vacuum 2.5m deep (18.3psia)
@23:15:37.26 Cartridge 14 filtering 823/1000ml at 0.20ml/s with 29.4psi vacuum 2.1m deep (17.8psia)
@23:16:37.26 Cartridge 14 filtering 836/1000ml at 0.20ml/s with 23.4psi vacuum 1.9m deep (17.6psia)
@23:17:37.26 Cartridge 14 filtering 848/1000ml at 0.21ml/s with 24.0psi vacuum 1.8m deep (17.4psia)
@23:18:37.26 Cartridge 14 filtering 861/1000ml at 0.24ml/s with 26.9psi vacuum 1.6m deep (17.1psia)
@23:19:37.26 Cartridge 14 filtering 873/1000ml at 0.23ml/s with 26.4psi vacuum 4.6m deep (21.5psia)
@23:20:37.26 Cartridge 14 filtering 885/1000ml at 0.24ml/s with 24.1psi vacuum 3.2m deep (19.4psia)
@23:21:37.26 Cartridge 14 filtering 898/1000ml at 0.20ml/s with 25.6psi vacuum 3.8m deep (20.4psia)
@23:22:37.26 Cartridge 14 filtering 910/1000ml at 0.20ml/s with 27.0psi vacuum 2.8m deep (18.9psia)
@23:22:56.17 <FILTERING> Flow averaged 0.25ml/s over 1:01:22
@23:22:56.20 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi
@23:22:56.76 Relieved excess vacuum in .6 seconds
@23:22:56.79 SamplePump.seek 1000ml,7:40
@23:23:01.51 Flow estimate reduced to 0.20ml/s
@23:23:01.53 <closeIntake> Intake.close
@23:23:01.58 <closeExhaust> Exhaust.close
@23:23:01.78 <FILTERING> Toroid.in :hold
@23:23:01.84 Toroid.seek :bypass
@23:23:03.46 Toroid.in :default
@23:23:03.53 Toroid.seek :clear
@23:23:05.14 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@23:23:05.15 Pressure.coast
@23:23:05.34 Sampled  915.1ml
@23:23:07.50 <LRAUV> -> Cmd.startProcessing
@23:23:07.87 <PROCESSING> Plunge.seek :reagent
@23:23:08.70 Sample loop closed. Ready to Deliver Reagents
@23:23:08.72 Archive.seek :sealed
@23:23:13.00 Archive.seek :evac
@23:23:26.94 delay 1:00
@23:24:26.98 Archive.seek :clear
@23:24:44.71 RNAlater soak for 10:00
@23:34:44.74 Archive.seek :tVent
@23:34:47.31 delay 1 second
@23:34:48.34 Archive.seek :evac
@23:35:03.95 delay 10 seconds
@23:35:13.99 Sample preserved and evacuated
@23:35:15.14 <LRAUV> -> Cmd.stop
@23:35:15.71 Carousel.seek 14
@23:35:15.88 Plunge.seek :locked
@23:35:16.83 <Archive> Archive.in :unlocking
@23:35:16.97 Archive.seek :clear
@23:35:17.00 <Air> Air.in :unlocking
@23:35:17.17 Air.seek :clear
@23:35:17.25 <Lysis1> Lysis1.in :unlocking
@23:35:17.36 Lysis1.seek :clear
@23:35:21.09 <LRAUV> Archive.coast
@23:35:21.14 Air.coast
@23:35:21.19 Lysis1.coast
@23:35:21.24 Lysis2.coast
@23:35:21.29 Plunge.coast
@23:35:21.34 SamplePump.coast
@23:35:21.39 Toroid.coast
@23:35:21.44 Carousel.coast
@23:35:21.49 PinPort.coast
@23:35:21.68 Gate.power :motor,:OFF
@23:35:21.69 Gate.power :core,:OFF
@23:35:21.71 Gate.power :analytic1,:OFF
@23:35:21.74 Gate.power :analytic2,:OFF
@23:35:21.75 Gate.power :analytic3,:OFF
@23:35:21.78 Gate.power :main,:OFF
@23:35:21.79 Safely stopped and ready to power off
##-- file closed 2021-04-25T06:47:38.222Z

##-- file opened 2021-04-25T06:47:38.224Z
@23:47:38.57 -> showStatus
@23:47:40.59 -> Cmd.loadCartridge -9
@23:47:40.73 Gate.power :main,:ON,limit to 5000mA
@23:47:40.74 Gate.power :analytic1,:ON,limit to 5000mA
@23:47:40.76 Gate.power :analytic2,:ON,limit to 5000mA
@23:47:40.79 Gate.power :analytic3,:ON,limit to 5000mA
@23:47:41.26 Powering core bus
@23:47:43.20 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@23:47:43.52 <LRAUV> Archive.configure :default
@23:47:43.64 Air.configure :default
@23:47:43.74 Lysis1.configure :default
@23:47:43.85 Lysis2.configure :default
@23:47:43.97 Plunge.configure :default
@23:47:44.10 SamplePump.configure :default
@23:47:44.24 ExtraPump.configure :default
@23:47:44.36 Toroid.configure :default
@23:47:44.48 Carousel.configure :default
@23:47:44.61 Pressure.configure :default
@23:47:44.73 PinPort.configure :default
@23:47:44.79 All elf controllers running firmware version 4.741
@23:47:44.81 Gate.power :motor,:ON,limit to 5000mA
@23:47:44.98 <Archive_home> Archive.in :default
@23:47:45.25 Archive.home.jog 0
@23:47:45.38 <Toroid_home> Toroid.in :default
@23:47:45.46 <Plunge_home> Plunge.in :default
@23:47:45.50 <Lysis1_home> Lysis1.in :default
@23:47:45.53 <PinPort_home> PinPort.in :default
@23:47:45.59 <Air_home> Air.in :default
@23:47:45.66 <Lysis2_home> Lysis2.in :default
@23:47:45.81 <Toroid_home> Toroid.home.jog 0
@23:47:45.86 <Lysis1_home> Lysis1.home.jog 0
@23:47:45.88 <Plunge_home> Plunge.home.jog 0
@23:47:45.91 <PinPort_home> PinPort.home.jog 0
@23:47:45.94 <Air_home> Air.home.jog 0
@23:47:45.98 <Lysis2_home> Lysis2.home.jog 0
@23:47:46.36 <Archive_home> Archive.in :homing
@23:47:46.55 <Toroid_home> Toroid.in :homing
@23:47:46.58 <Plunge_home> Plunge.in :homing
@23:47:46.63 <Archive_home> Archive.jog 150
@23:47:46.68 <Lysis1_home> Lysis1.in :homing
@23:47:46.72 <PinPort_home> PinPort.in :homing
@23:47:46.89 <Toroid_home> Toroid.jog 624
@23:47:46.92 <Plunge_home> Plunge.jog 1575
@23:47:47.01 <PinPort_home> PinPort.jog -106
@23:47:47.06 <Lysis1_home> Lysis1.jog 1416
@23:47:47.19 <Air_home> Air.in :homing
@23:47:47.21 <Lysis2_home> Lysis2.in :homing
@23:47:47.29 <Air_home> Air.jog 156
@23:47:47.37 <Lysis2_home> Lysis2.jog 1430
@23:47:47.45 <PinPort_home> PinPort.setRawPosition! 0
@23:47:47.47 <Archive_home> Archive.setRawPosition! 50
@23:47:47.54 <PinPort_home> PinPort.seek :home
@23:47:47.59 <Archive_home> Archive.seek :home
@23:47:47.72 <PinPort_home> PinPort.in :default
@23:47:47.80 <Air_home> Air.setRawPosition! 50
@23:47:47.84 Air.seek :home
@23:47:47.93 <Archive_home> Archive.in :default
@23:47:48.12 <Air_home> Air.in :default
@23:47:48.21 <Plunge_home> Plunge.setRawPosition! 50
@23:47:48.27 Plunge.seek :home
@23:47:48.35 <Lysis1_home> Lysis1.setRawPosition! 50
@23:47:48.40 Lysis1.seek :home
@23:47:48.55 <Lysis2_home> Lysis2.setRawPosition! 50
@23:47:48.60 Lysis2.seek :home
@23:47:48.70 <Plunge_home> Plunge.in :default
@23:47:48.72 <Lysis1_home> Lysis1.in :default
@23:47:48.88 <Lysis2_home> Lysis2.in :default
@23:47:49.08 <Toroid_home> Toroid.setRawPosition! 0
@23:47:49.12 Toroid.seek :home
@23:47:49.22 Toroid.in :default
@23:47:49.35 <LRAUV> Toroid.seek :clear
@23:47:50.97 Carousel.in :default
@23:47:51.03 Carousel.home.jog 0
@23:47:51.54 Carousel.in :default
@23:47:51.60 Carousel.seek 14
@23:47:51.76 Selecting Cartridge 9
@23:47:52.00 Carousel.seek 9
@23:47:53.29 Lysis1.in :fast
@23:47:53.35 Lysis1.seek :locked
@23:47:55.95 <Plunge> Plunge.seek :locked
@23:47:56.07 <Air> Air.in :fast
@23:47:56.13 Air.seek :locked
@23:47:58.73 <LRAUV> Powering cartridge bus
@23:48:00.85 Gate.setIR 1750,5000,0,6
@23:48:00.98 Gate.setIR 0,5000,0,6
@23:48:01.04 PuckHeater.configure :default
@23:48:01.08 Gate.power :heat,:ON,limit to 5000mA
@23:48:01.75 Mounted lysis Cartridge board#Q05057
@23:48:01.78 Using default puck heat loss curve for HeatingAccelInCarousel
@23:48:20.99 -> Cmd.startFiltering
@23:48:21.44 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@23:48:21.45 SamplePump.setPosition! 0ml
@23:48:21.49 Bypass.open
@23:48:23.01 Intake.open
@23:48:23.80 Sea 5.7m deep (23.1psia)
@23:48:23.81 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@23:48:26.41 Vacuum tare is 51% of full scale
@23:48:26.58 Pressure.in :default
@23:48:26.64 Pressure.hold
@23:48:26.81 SamplePump.in :prime
@23:48:26.88 SamplePump.seek 10ml,1:21
@23:48:36.26 Pressure.coast
@23:48:36.31 SamplePump.setPosition! 0ml
@23:48:36.36 Exhaust.open
@23:48:37.78 <closeBypass> Bypass.close
@23:48:39.33 <FILTERING> Pressure.in :default
@23:48:39.39 Pressure.hold
@23:48:39.50 Sea 6.6m deep (24.4psia)
@23:48:39.63 SamplePump.in :prime
@23:48:39.69 SamplePump.seek 100ml,8:51
@23:50:49.57 Pressure.coast
@23:50:49.63 Bypass.open
@23:50:50.33 Intake.close
@23:50:51.20 Toroid.in :hold
@23:50:51.26 Toroid.seek :crack
@23:50:52.84 Toroid.seek :cartridge
@23:50:53.94 Toroid.in :default
@23:50:54.00 Toroid.seek :clear
@23:50:55.96 Pressurizing cartridge to 26psi above 24.7psia
@23:50:55.97 SamplePump.setPosition! 0ml
@23:50:56.01 Intake.open
@23:50:57.15 Bypass.close
@23:50:57.89 Exhaust.close
@23:50:58.73 Pressure.in :default
@23:50:58.79 Pressure.hold
@23:50:58.96 SamplePump.in :charge
@23:50:59.03 SamplePump.seek 2.3ml,42 seconds
@23:51:07.95 Pressure.coast
@23:51:08.00 Intake.close
@23:51:08.83 Pressurized cartridge with 0.352ml to 26.8psi
@23:51:08.84 Isolated @46.5psia (21.8psi)
@23:51:08.85 Verifying cartridge holds at least 0.75..0.85 of 46.5psia
@23:51:08.88 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@23:51:13.50 Sampling 1000.0ml
@23:51:13.52 SamplePump.setPosition! 0ml
@23:51:13.57 Exhaust.open
@23:51:14.31 Intake.open
@23:51:15.22 Pressure.in :default
@23:51:15.28 Pressure.hold
@23:51:15.39 Sea 7.6m deep (25.9psia)
@23:51:15.53 SamplePump.in :sample
@23:51:15.59 SamplePump.seek 1000ml,1:23:53
@23:51:18.26 <sampling> Cartridge 9 filtering 1/1000ml at 0.36ml/s with 22.7psi vacuum 4.1m deep (20.7psia)
@23:52:18.25 Cartridge 9 filtering 20/1000ml at 0.34ml/s with 28.7psi vacuum 1.7m deep (17.2psia)
@23:53:18.26 Cartridge 9 filtering 39/1000ml at 0.35ml/s with 25.6psi vacuum 4.7m deep (21.6psia)
@23:54:18.25 Cartridge 9 filtering 58/1000ml at 0.31ml/s with 22.2psi vacuum 2.0m deep (17.6psia)
@23:55:18.26 Cartridge 9 filtering 77/1000ml at 0.26ml/s with 27.4psi vacuum 3.6m deep (19.9psia)
@23:56:18.25 Cartridge 9 filtering 95/1000ml at 0.31ml/s with 28.0psi vacuum 3.1m deep (19.2psia)
@23:57:18.26 Cartridge 9 filtering 113/1000ml at 0.32ml/s with 25.8psi vacuum 4.6m deep (21.5psia)
@23:58:18.25 Cartridge 9 filtering 130/1000ml at 0.25ml/s with 25.1psi vacuum 2.4m deep (18.3psia)
@23:59:18.26 Cartridge 9 filtering 148/1000ml at 0.33ml/s with 22.8psi vacuum 4.5m deep (21.4psia)
@00:00:18.25PDT25-Apr-21 Cartridge 9 filtering 165/1000ml at 0.32ml/s with 23.1psi vacuum 4.2m deep (21.0psia)
@00:01:18.26 Cartridge 9 filtering 182/1000ml at 0.24ml/s with 26.4psi vacuum 1.6m deep (17.1psia)
@00:02:18.25 Cartridge 9 filtering 199/1000ml at 0.31ml/s with 27.3psi vacuum 3.5m deep (19.9psia)
@00:03:18.26 Cartridge 9 filtering 216/1000ml at 0.24ml/s with 24.8psi vacuum
@00:04:18.25 Cartridge 9 filtering 233/1000ml at 0.31ml/s with 24.5psi vacuum 6.0m deep (23.6psia)
@00:05:18.26 Cartridge 9 filtering 249/1000ml at 0.22ml/s with 24.4psi vacuum
@00:06:18.25 Cartridge 9 filtering 265/1000ml at 0.30ml/s with 22.6psi vacuum 5.3m deep (22.5psia)
@00:07:18.26 Cartridge 9 filtering 282/1000ml at 0.29ml/s with 26.9psi vacuum 1.7m deep (17.2psia)
@00:08:18.25 Cartridge 9 filtering 298/1000ml at 0.29ml/s with 24.7psi vacuum 4.2m deep (21.0psia)
@00:09:18.26 Cartridge 9 filtering 314/1000ml at 0.30ml/s with 24.1psi vacuum 3.7m deep (20.2psia)
@00:10:18.25 Cartridge 9 filtering 329/1000ml at 0.28ml/s with 23.3psi vacuum 3.0m deep (19.2psia)
@00:11:18.26 Cartridge 9 filtering 345/1000ml at 0.28ml/s with 24.8psi vacuum 5.1m deep (22.2psia)
@00:12:18.25 Cartridge 9 filtering 360/1000ml at 0.24ml/s with 19.4psi vacuum 2.0m deep (17.7psia)
@00:13:18.26 Cartridge 9 filtering 375/1000ml at 0.27ml/s with 23.4psi vacuum 5.9m deep (23.4psia)
@00:14:18.25 Cartridge 9 filtering 391/1000ml at 0.21ml/s with 20.7psi vacuum 2.0m deep (17.7psia)
@00:15:18.26 Cartridge 9 filtering 406/1000ml at 0.27ml/s with 25.0psi vacuum 4.1m deep (20.8psia)
@00:16:18.25 Cartridge 9 filtering 420/1000ml at 0.26ml/s with 22.3psi vacuum 3.5m deep (19.9psia)
@00:17:18.26 Cartridge 9 filtering 435/1000ml at 0.24ml/s with 27.4psi vacuum 3.2m deep (19.4psia)
@00:18:18.25 Cartridge 9 filtering 450/1000ml at 0.23ml/s with 28.5psi vacuum 4.1m deep (20.7psia)
@00:19:18.26 Cartridge 9 filtering 464/1000ml at 0.27ml/s with 22.9psi vacuum 2.5m deep (18.4psia)
@00:20:18.25 Cartridge 9 filtering 479/1000ml at 0.23ml/s with 28.0psi vacuum 4.7m deep (21.7psia)
@00:21:18.26 Cartridge 9 filtering 493/1000ml at 0.24ml/s with 27.8psi vacuum 1.6m deep (17.0psia)
@00:22:18.25 Cartridge 9 filtering 507/1000ml at 0.24ml/s with 22.6psi vacuum 4.8m deep (21.8psia)
@00:23:18.26 Cartridge 9 filtering 521/1000ml at 0.26ml/s with 22.1psi vacuum 3.8m deep (20.4psia)
@00:24:18.25 Cartridge 9 filtering 535/1000ml at 0.24ml/s with 24.5psi vacuum 3.3m deep (19.6psia)
@00:25:18.26 Cartridge 9 filtering 549/1000ml at 0.20ml/s with 25.6psi vacuum 2.5m deep (18.3psia)
@00:26:18.25 Cartridge 9 filtering 563/1000ml at 0.21ml/s with 21.0psi vacuum 2.7m deep (18.7psia)
@00:27:18.26 Cartridge 9 filtering 576/1000ml at 0.20ml/s with 23.8psi vacuum 4.6m deep (21.5psia)
@00:28:18.25 Cartridge 9 filtering 590/1000ml at 0.26ml/s with 24.1psi vacuum 2.1m deep (17.8psia)
@00:29:18.26 Cartridge 9 filtering 603/1000ml at 0.20ml/s with 26.1psi vacuum 4.0m deep (20.6psia)
@00:30:18.25 Cartridge 9 filtering 617/1000ml at 0.20ml/s with 24.8psi vacuum
@00:31:18.26 Cartridge 9 filtering 630/1000ml at 0.20ml/s with 26.9psi vacuum 4.0m deep (20.7psia)
@00:32:18.25 Cartridge 9 filtering 643/1000ml at 0.23ml/s with 24.1psi vacuum 3.6m deep (20.0psia)
@00:33:18.26 Cartridge 9 filtering 656/1000ml at 0.24ml/s with 26.5psi vacuum 3.0m deep (19.1psia)
@00:34:18.25 Cartridge 9 filtering 669/1000ml at 0.22ml/s with 26.4psi vacuum 3.2m deep (19.4psia)
@00:35:18.26 Cartridge 9 filtering 683/1000ml at 0.24ml/s with 23.3psi vacuum 2.8m deep (18.8psia)
@00:36:18.25 Cartridge 9 filtering 695/1000ml at 0.20ml/s with 26.2psi vacuum 4.1m deep (20.7psia)
@00:37:18.26 Cartridge 9 filtering 708/1000ml at 0.22ml/s with 23.5psi vacuum 2.2m deep (17.9psia)
@00:38:18.25 Cartridge 9 filtering 721/1000ml at 0.21ml/s with 23.0psi vacuum 5.2m deep (22.3psia)
@00:39:18.26 Cartridge 9 filtering 734/1000ml at 0.21ml/s with 23.9psi vacuum 3.5m deep (19.9psia)
@00:40:18.25 Cartridge 9 filtering 746/1000ml at 0.21ml/s with 26.7psi vacuum 2.2m deep (18.0psia)
@00:41:18.26 Cartridge 9 filtering 759/1000ml at 0.20ml/s with 22.5psi vacuum 4.9m deep (21.9psia)
@00:42:18.25 Cartridge 9 filtering 772/1000ml at 0.21ml/s with 24.9psi vacuum 2.3m deep (18.1psia)
@00:43:18.26 Cartridge 9 filtering 784/1000ml at 0.21ml/s with 24.3psi vacuum 5.0m deep (22.1psia)
@00:44:18.25 Cartridge 9 filtering 796/1000ml at 0.21ml/s with 24.1psi vacuum 1.8m deep (17.3psia)
@00:45:18.26 Cartridge 9 filtering 809/1000ml at 0.20ml/s with 27.1psi vacuum 4.5m deep (21.4psia)
@00:46:18.25 Cartridge 9 filtering 821/1000ml at 0.22ml/s with 23.2psi vacuum 2.5m deep (18.4psia)
@00:47:18.26 Cartridge 9 filtering 833/1000ml at 0.20ml/s with 27.4psi vacuum 3.6m deep (20.0psia)
@00:48:18.25 Cartridge 9 filtering 846/1000ml at 0.20ml/s with 25.1psi vacuum 3.2m deep (19.5psia)
@00:49:18.26 Cartridge 9 filtering 858/1000ml at 0.21ml/s with 25.5psi vacuum 3.0m deep (19.2psia)
@00:50:18.25 Cartridge 9 filtering 870/1000ml at 0.20ml/s with 27.9psi vacuum 5.2m deep (22.4psia)
@00:51:18.26 Cartridge 9 filtering 882/1000ml at 0.20ml/s with 29.7psi vacuum 1.8m deep (17.3psia)
@00:51:27.77 <FILTERING> Flow averaged 0.24ml/s over 1:00:13
@00:51:27.80 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi
@00:51:28.37 Relieved excess vacuum in .6 seconds
@00:51:28.40 SamplePump.seek 1000ml,10:10
@00:51:34.68 Flow estimate reduced to 0.20ml/s
@00:51:34.71 <closeIntake> Intake.close
@00:51:34.76 <closeExhaust> Exhaust.close
@00:51:34.94 <FILTERING> Toroid.in :hold
@00:51:35.00 Toroid.seek :bypass
@00:51:36.63 Toroid.in :default
@00:51:36.69 Toroid.seek :clear
@00:51:38.30 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@00:51:38.31 Pressure.coast
@00:51:38.50 Sampled  885.4ml
@00:51:40.75 <LRAUV> -> Cmd.startProcessing
@00:51:42.18 <PROCESSING> SPR.command \"read_sequences\"
@00:51:42.23 SPR.command \"stats_on\"
@00:51:42.27 SPR.command \"start 0 1\"
@00:51:44.28 <SPRlogger> Begun log /var/log/esp/spr21apr2500h.spr
@00:51:45.08 <PROCESSING> Handoff.select :waste
@00:51:51.76 Syringe tare is 20% of full scale
@00:51:51.78 PinPort.in :locking
@00:51:51.86 PinPort.seek :locked
@00:51:52.34 <posArchive> Archive.in :fast
@00:51:52.40 <posLyS2> Lysis2.in :fast
@00:51:52.45 <PROCESSING> Lysis1.seek 1350uL
@00:51:52.56 <posArchive> Archive.seek :full
@00:51:52.61 <posLyS2> Lysis2.seek :full
@00:51:54.69 <PROCESSING> Plunge.seek :reagent
@00:51:55.59 Evacuating puck with filter bubble point of 50psi
@00:51:55.61 PinPort.seek :locked
@00:51:55.69 Handoff.select :waste
@00:52:02.28 Air syringe pumping up to 1 stroke to reach 8psi
@00:52:02.29 Air stroke #1 of 1
@00:52:02.38 Air.in :pressurize
@00:52:02.44 Air.seek :pressurized
@00:52:13.69 -0.0psi after pressurize
@00:52:13.70 Air.in :compress
@00:52:13.77 Air.seek :evac
@00:52:17.86 0.7psi after compress
@00:52:30.43 0.2psi after compress (stable)
@00:52:30.45 Air.in :default
@00:52:30.52 Air.hold
@00:52:30.69 at 0.1psi Opened to waste
@00:52:30.70 Handoff.select :blocked
@00:52:30.83 Archive syringe pumping up to 5 strokes to reach 50psi
@00:52:30.84 Archive stroke #1 of 5
@00:52:30.92 Archive.in :pressurize
@00:52:31.00 Archive.seek :pressurized
@00:52:43.74 5.7psi after pressurize
@00:52:43.75 Archive.in :compress
@00:52:43.83 Archive.seek :empty
@00:52:59.70 28.1psi after compress
@00:53:33.79 45.6psi after compress (stable)
@00:53:33.80 Archive.in :gulp
@00:53:33.87 Archive.seek :topVent
@00:53:41.08 47.4psi after gulp
@00:53:53.65 47.8psi after gulp (stable)
@00:53:53.67 Archive stroke #2 of 5
@00:53:53.76 Archive.in :pressurize
@00:53:53.82 Archive.seek :pressurized
@00:54:06.56 47.1psi after pressurize
@00:54:06.58 Archive.in :compress
@00:54:06.65 Archive.seek :empty
@00:54:19.82 Reached 50.3 of 50.0psi after pumping 3402uL (1.97 strokes)
@00:54:19.83 Archive.in :default
@00:54:19.90 Archive.hold
@00:54:20.00 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position
@00:54:20.02 Handoff.select :waste
@00:54:32.22 delay 1:00
@00:55:32.24 Handoff.select :blocked
@00:55:54.91 Handoff.select :waste
@00:55:55.03 -0.1psi at evacEnd
@00:55:55.04 Handoff.select :blocked
@00:55:55.16 Archive.in :fast
@00:55:55.23 Archive.seek :topVent
@00:56:08.75 Stable @0.1psi after Handoff blocked
@00:56:08.77 Archive syringe pumping up to 5 strokes to reach 30psi
@00:56:08.78 Archive stroke #1 of 5
@00:56:08.88 Archive.in :pressurize
@00:56:08.94 Archive.seek :pressurized
@00:56:21.67 0.2psi after pressurize
@00:56:21.68 Archive.in :compress
@00:56:21.78 Archive.seek :empty
@00:56:37.66 0.4psi after compress
@00:56:50.23 1.1psi after compress (stable)
@00:56:50.25 Archive.in :gulp
@00:56:50.32 Archive.seek :topVent
@00:56:57.54 2.2psi after gulp
@00:57:10.11 3.4psi after gulp (stable)
@00:57:10.12 Archive stroke #2 of 5
@00:57:10.21 Archive.in :pressurize
@00:57:10.28 Archive.seek :pressurized
@00:57:23.01 5.1psi after pressurize
@00:57:23.02 Archive.in :compress
@00:57:23.10 Archive.seek :empty
@00:57:38.98 7.1psi after compress
@00:58:20.27 Reached 30.3 of 30.1psi after pumping 3460uL (2.00 strokes)
@00:58:20.28 Archive.in :default
@00:58:20.35 Archive.hold
@00:58:20.41 Handoff.select :waste
@00:58:37.09 delay 1:00
@00:59:37.12 Handoff.select :blocked
@01:00:02.86 Pressure too High, open to waste again (8.0psi)
@01:00:02.87 Handoff.select :waste
@01:00:03.00 delay 1:00
@01:01:03.02 Handoff.select :blocked
@01:01:30.69 Handoff.select :waste
@01:01:30.81 3.8psi at evacEnd
@01:01:30.82 Air.seek :fullVent
@01:01:32.27 Archive.in :fast
@01:01:32.33 Archive.seek :topVent
@01:01:39.47 Seawater evacuated, Presure above/below filter released. Lysis begins
@01:01:39.58 Lysis1.in :slow
@01:01:39.64 Lysis1.seek 1050uL
@01:02:09.67 delay 5 seconds
@01:02:14.69 Handoff.select :blocked
@01:02:14.86 Lysis1.seek 450uL
@01:03:14.78 delay 3 seconds
@01:03:17.85 Air.in to mix into 1.37806 parts Lysis1
@01:03:18.42 <Airmixer> Air.seek :evac
@01:03:18.46 <PROCESSING> Lysis1.seek 300uL
@01:03:33.55 Lysis1.seek :empty
@01:04:03.63 Lysis buffer delivered @14.6psi
@01:04:03.64 Polling Can every 2:00
@01:04:04.19 PuckHeater.configure :default #ambient 11.8C
@01:04:04.25 PuckHeater.seek 80.2C
@01:05:20.07 PuckHeater.hold 2:00,80.2C
@01:07:20.33 Polling Can every 2:00
@01:07:20.88 PuckHeater.configure :default #ambient 11.9C
@01:07:20.92 PuckHeater.hold 30 seconds,79.2C
@01:07:51.19 Polling Can every 2:00
@01:07:51.75 PuckHeater.configure :default #ambient 11.9C
@01:07:51.79 PuckHeater.hold 30 seconds,78.6C
@01:08:22.01 Polling Can every 2:00
@01:08:22.55 PuckHeater.configure :default #ambient 11.9C
@01:08:22.60 PuckHeater.hold 10:00,78.1C
@01:18:23.49 Lysis complete. Syringe pressure @14.2psi
@01:18:23.50 Handoff.select :blocked
@01:18:23.54 Archive syringe pumping up to 3 strokes to reach 40psi
@01:18:23.55 Archive stroke #1 of 3
@01:18:23.63 Archive.in :pressurize
@01:18:23.70 Archive.seek :pressurized
@01:18:36.43 13.9psi after pressurize
@01:18:36.44 Archive.in :compress
@01:18:36.52 Archive.seek :empty
@01:18:45.71 Reached 40.4 of 40.0psi after pumping 1587uL (0.92 strokes)
@01:18:45.73 Archive.in :default
@01:18:45.81 Archive.hold
@01:18:45.88 delay 2 seconds
@01:18:47.90 Archive.in :fast
@01:18:47.97 Archive.seek :full
@01:18:54.68 Lysis1.seek 138uL
@01:19:08.55 delay 1 second
@01:19:09.58 <fillArchive> Archive.seek :full
@01:19:09.60 <PROCESSING> Lysis1.seek :empty
@01:19:23.62 Initial air pressure @52.4psi
@01:19:23.69 Lysis1.in :extract
@01:19:23.76 Lysis1.seek 600uL,nil
@01:19:25.55 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@01:19:25.56 Archive stroke #1 of 6
@01:19:25.65 Archive.in :pressurize
@01:19:25.71 Archive.seek :pressurized
@01:19:38.44 16.1psi after pressurize
@01:19:38.45 Archive.in :compress
@01:19:38.52 Archive.seek :empty
@01:19:54.43 15.4psi after compress
@01:20:07.03 15.9psi after compress (stable)
@01:20:07.49 Archive.in :default
@01:20:07.54 Archive.hold
@01:20:07.66 <PROCESSING> Initial 600uL lysate @15.7psi
@01:20:07.67 Handoff.select :blocked
@01:20:07.76 Initial air pressure @15.9psi
@01:20:07.83 Lysis1.seek 825uL,nil
@01:20:07.90 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@01:20:07.92 Archive stroke #1 of 6
@01:20:08.06 Archive.in :compress
@01:20:08.13 Archive.seek :empty
@01:20:08.31 16.2psi after compress
@01:20:20.89 15.8psi after compress (stable)
@01:20:26.08 Archive.in :gulp
@01:20:26.14 Archive.seek :topVent
@01:20:33.38 15.9psi after gulp
@01:20:45.96 15.8psi after gulp (stable)
@01:20:45.97 Archive stroke #2 of 6
@01:20:46.07 Archive.in :pressurize
@01:20:46.13 Archive.seek :pressurized
@01:20:58.89 15.9psi after pressurize
@01:20:58.90 Archive.in :compress
@01:20:58.98 Archive.seek :empty
@01:21:14.88 15.4psi after compress
@01:21:22.67 Archive.in :default
@01:21:22.73 Archive.hold
@01:21:22.93 <PROCESSING> Recovered 825uL lysate @15.8psi
@01:21:22.95 Air.in :halfSpeed
@01:21:23.02 Air.seek :fullVent
@01:21:25.27 Puck Top Vented, bottom @4.2psi
@01:21:25.28 Handoff.select :waste
@01:21:25.40 delay 5 seconds
@01:21:30.46 Handoff open to waste, bottom @0.1psi
@01:21:30.47 Air.in :default
@01:21:30.54 Air.seek :evac
@01:21:31.98 Lysis2.in :DAmix
@01:21:32.08 Lysis1.in :DAmix
@01:21:32.63 <Lysis2mixer> Lysis2.seek 1480uL
@01:21:32.69 <PROCESSING> Lysis1.seek 575uL
@01:22:07.72 delay 5 seconds
@01:22:12.74 Handoff.select SPR
@01:22:12.87 SPR.command \"start 2 14\"
@01:22:14.42 delay 10 seconds
@01:22:24.44 SPR.command \"start 1 0\"
@01:22:27.67 Waiting up to 4:00 for SPR to enter state 1
@01:24:30.06 SPR.command \"hold_on\"
@01:24:31.95 SPR Dilution 1 of 1 -- Running Experiment 1
@01:24:32.37 <Lysis2mixer> Lysis2.seek 1180uL
@01:24:32.42 <PROCESSING> Lysis1.seek 275uL
@01:25:14.47 volL1 = 300.0 and volL2 = 300.0
@01:25:14.50 delay 15 seconds
@01:25:29.53 SPR.command \"hold_off\"
@01:25:32.11 Waiting up to 38:00 for SPR to enter state 7
@01:52:14.71 SPR Analysis has Completed
@01:52:14.73 Handoff.select :waste
@01:52:15.65 <SPRlogger> Ended log /var/log/esp/spr21apr2500h.spr
@01:52:15.66 <PROCESSING> SPR.command \"stats_off\"
@01:52:17.31 <LRAUV> -> Cmd.stop
@01:52:17.40 <closeHandoff> Handoff.select :close
@01:52:17.52 <LRAUV> Gate.power :heat,:OFF
@01:52:17.54 Gate.power :cartridge,:OFF
@01:52:17.62 Carousel.seek 9
@01:52:17.79 Plunge.seek :locked
@01:52:18.68 PinPort.in :unlocking
@01:52:18.74 PinPort.seek :clear
@01:52:19.27 <Archive> Archive.in :unlocking
@01:52:19.41 Archive.seek :clear
@01:52:19.44 <Air> Air.in :unlocking
@01:52:19.58 Air.seek :clear
@01:52:19.66 <Lysis1> Lysis1.in :unlocking
@01:52:19.78 Lysis1.seek :clear
@01:52:19.85 <Lysis2> Lysis2.in :unlocking
@01:52:19.92 Lysis2.seek :clear
@01:52:23.52 <LRAUV> Archive.coast
@01:52:23.57 Air.coast
@01:52:23.62 Lysis1.coast
@01:52:23.67 Lysis2.coast
@01:52:23.72 Plunge.coast
@01:52:23.77 SamplePump.coast
@01:52:23.82 Toroid.coast
@01:52:23.87 Carousel.coast
@01:52:23.92 PinPort.coast
@01:52:24.10 Gate.power :motor,:OFF
@01:52:24.12 Gate.power :core,:OFF
@01:52:24.14 Gate.power :analytic1,:OFF
@01:52:24.16 Gate.power :analytic2,:OFF
@01:52:24.18 Gate.power :analytic3,:OFF
@01:52:24.20 Gate.power :main,:OFF
@01:52:24.22 Safely stopped and ready to power off
##-- file closed 2021-04-25T11:02:00.131Z

##-- file opened 2021-04-25T11:02:00.133Z
@04:02:00.56 -> showStatus
@04:02:02.59 -> Cmd.loadCartridge -6
@04:02:02.75 Gate.power :main,:ON,limit to 5000mA
@04:02:02.76 Gate.power :analytic1,:ON,limit to 5000mA
@04:02:02.78 Gate.power :analytic2,:ON,limit to 5000mA
@04:02:02.80 Gate.power :analytic3,:ON,limit to 5000mA
@04:02:02.93 Powering core bus
@04:02:05.23 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@04:02:05.36 <LRAUV> Archive.configure :default
@04:02:05.48 Air.configure :default
@04:02:05.59 Lysis1.configure :default
@04:02:05.70 Lysis2.configure :default
@04:02:05.82 Plunge.configure :default
@04:02:05.94 SamplePump.configure :default
@04:02:06.09 ExtraPump.configure :default
@04:02:06.21 Toroid.configure :default
@04:02:06.33 Carousel.configure :default
@04:02:06.46 Pressure.configure :default
@04:02:06.57 PinPort.configure :default
@04:02:06.63 All elf controllers running firmware version 4.741
@04:02:06.65 Gate.power :motor,:ON,limit to 5000mA
@04:02:06.81 <Archive_home> Archive.in :default
@04:02:07.06 Archive.home.jog 0
@04:02:07.23 <PinPort_home> PinPort.in :default
@04:02:07.29 <Toroid_home> Toroid.in :default
@04:02:07.33 <Plunge_home> Plunge.in :default
@04:02:07.36 <Lysis1_home> Lysis1.in :default
@04:02:07.40 <Air_home> Air.in :default
@04:02:07.45 <Lysis2_home> Lysis2.in :default
@04:02:07.64 <PinPort_home> PinPort.home.jog 0
@04:02:07.68 <Toroid_home> Toroid.home.jog 0
@04:02:07.71 <Lysis1_home> Lysis1.home.jog 0
@04:02:07.74 <Plunge_home> Plunge.home.jog 0
@04:02:07.76 <Air_home> Air.home.jog 0
@04:02:07.80 <Lysis2_home> Lysis2.home.jog 0
@04:02:08.19 <Archive_home> Archive.in :homing
@04:02:08.42 <PinPort_home> PinPort.in :homing
@04:02:08.46 <Toroid_home> Toroid.in :homing
@04:02:08.51 <Air_home> Air.in :homing
@04:02:08.57 <Plunge_home> Plunge.in :homing
@04:02:08.60 <Archive_home> Archive.jog 154
@04:02:08.65 <Lysis1_home> Lysis1.in :homing
@04:02:08.78 <PinPort_home> PinPort.jog -107
@04:02:08.84 <Toroid_home> Toroid.jog 624
@04:02:08.88 <Air_home> Air.jog 169
@04:02:08.91 <Plunge_home> Plunge.jog 1585
@04:02:08.95 <Lysis1_home> Lysis1.jog 1415
@04:02:09.17 <Lysis2_home> Lysis2.in :homing
@04:02:09.19 <PinPort_home> PinPort.setRawPosition! 0
@04:02:09.26 PinPort.seek :home
@04:02:09.30 <Lysis2_home> Lysis2.jog 150
@04:02:09.44 <Archive_home> Archive.setRawPosition! 50
@04:02:09.46 <PinPort_home> PinPort.in :default
@04:02:09.54 <Archive_home> Archive.seek :home
@04:02:09.64 <Air_home> Air.setRawPosition! 50
@04:02:09.68 Air.seek :home
@04:02:09.77 <Lysis2_home> Lysis2.setRawPosition! 50
@04:02:09.83 Lysis2.seek :home
@04:02:09.91 <Archive_home> Archive.in :default
@04:02:10.00 <Air_home> Air.in :default
@04:02:10.10 <Lysis2_home> Lysis2.in :default
@04:02:10.22 <Lysis1_home> Lysis1.setRawPosition! 50
@04:02:10.27 Lysis1.seek :home
@04:02:10.36 <Plunge_home> Plunge.setRawPosition! 50
@04:02:10.40 Plunge.seek :home
@04:02:10.55 <Lysis1_home> Lysis1.in :default
@04:02:10.68 <Plunge_home> Plunge.in :default
@04:02:11.09 <Toroid_home> Toroid.setRawPosition! 0
@04:02:11.14 Toroid.seek :home
@04:02:11.22 Toroid.in :default
@04:02:11.35 <LRAUV> Toroid.seek :clear
@04:02:12.96 Carousel.in :default
@04:02:13.02 Carousel.home.jog 0
@04:02:13.52 Carousel.in :default
@04:02:13.58 Carousel.seek 9
@04:02:13.75 Selecting Cartridge 12
@04:02:13.99 Carousel.seek 12
@04:02:14.88 Lysis1.in :fast
@04:02:14.95 Lysis1.seek :locked
@04:02:17.55 <Plunge> Plunge.seek :locked
@04:02:17.68 <Air> Air.in :fast
@04:02:17.74 Air.seek :locked
@04:02:20.34 <LRAUV> Powering cartridge bus
@04:02:22.46 Gate.setIR 1750,5000,0,6
@04:02:22.60 Gate.setIR 0,5000,0,6
@04:02:22.65 Gate.power :cartridge,:OFF
@04:02:23.18 Mounted archive Cartridge
@04:02:42.18 -> Cmd.startFiltering
@04:02:42.64 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@04:02:42.66 SamplePump.setPosition! 0ml
@04:02:42.70 Bypass.open
@04:02:44.19 Intake.open
@04:02:44.44 Sea 6.7m deep (24.6psia)
@04:02:44.45 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@04:02:47.03 Vacuum tare is 51% of full scale
@04:02:47.16 Pressure.in :default
@04:02:47.23 Pressure.hold
@04:02:47.41 SamplePump.in :prime
@04:02:47.47 SamplePump.seek 10ml,1:21
@04:02:56.86 Pressure.coast
@04:02:56.91 SamplePump.setPosition! 0ml
@04:02:56.96 Exhaust.open
@04:02:58.39 <closeBypass> Bypass.close
@04:02:59.92 <FILTERING> Pressure.in :default
@04:02:59.98 Pressure.hold
@04:03:00.09 Sea 5.9m deep (23.4psia)
@04:03:00.22 SamplePump.in :prime
@04:03:00.29 SamplePump.seek 100ml,8:51
@04:05:10.01 Pressure.coast
@04:05:10.07 Bypass.open
@04:05:10.77 Intake.close
@04:05:11.60 Toroid.in :hold
@04:05:11.66 Toroid.seek :crack
@04:05:13.23 Toroid.seek :cartridge
@04:05:14.32 Toroid.in :default
@04:05:14.38 Toroid.seek :clear
@04:05:16.35 Pressurizing cartridge to 26psi above 24.2psia
@04:05:16.37 SamplePump.setPosition! 0ml
@04:05:16.42 Intake.open
@04:05:17.53 Bypass.close
@04:05:18.27 Exhaust.close
@04:05:19.08 Pressure.in :default
@04:05:19.14 Pressure.hold
@04:05:19.31 SamplePump.in :charge
@04:05:19.38 SamplePump.seek 2.3ml,42 seconds
@04:05:25.40 Pressure.coast
@04:05:25.45 Intake.close
@04:05:26.26 Pressurized cartridge with 0.158ml to 26.7psi
@04:05:26.27 Isolated @44.3psia (20.1psi)
@04:05:26.28 Verifying cartridge holds at least 0.75..0.85 of 44.3psia
@04:05:26.31 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@04:05:30.94 Sampling 1000.0ml
@04:05:30.95 SamplePump.setPosition! 0ml
@04:05:31.00 Exhaust.open
@04:05:31.69 Intake.open
@04:05:32.54 Pressure.in :default
@04:05:32.60 Pressure.hold
@04:05:32.72 Sea 5.7m deep (23.1psia)
@04:05:32.85 SamplePump.in :sample
@04:05:32.92 SamplePump.seek 1000ml,1:23:53
@04:05:35.58 <sampling> Cartridge 12 filtering 1/1000ml at 0.31ml/s with 20.5psi vacuum 3.9m deep (20.5psia)
@04:06:35.59 Cartridge 12 filtering 21/1000ml at 0.31ml/s with 19.4psi vacuum 3.7m deep (20.2psia)
@04:07:35.59 Cartridge 12 filtering 40/1000ml at 0.34ml/s with 26.3psi vacuum 6.0m deep (23.6psia)
@04:08:35.59 Cartridge 12 filtering 60/1000ml at 0.34ml/s with 21.5psi vacuum
@04:09:35.59 Cartridge 12 filtering 79/1000ml at 0.27ml/s with 21.2psi vacuum 3.5m deep (19.8psia)
@04:10:35.59 Cartridge 12 filtering 97/1000ml at 0.26ml/s with 25.9psi vacuum
@04:11:35.59 Cartridge 12 filtering 116/1000ml at 0.27ml/s with 27.1psi vacuum 1.8m deep (17.3psia)
@04:12:35.59 Cartridge 12 filtering 134/1000ml at 0.32ml/s with 22.0psi vacuum 5.3m deep (22.6psia)
@04:13:35.59 Cartridge 12 filtering 152/1000ml at 0.26ml/s with 29.1psi vacuum
@04:14:35.59 Cartridge 12 filtering 170/1000ml at 0.30ml/s with 25.7psi vacuum 5.8m deep (23.3psia)
@04:15:35.59 Cartridge 12 filtering 188/1000ml at 0.26ml/s with 20.8psi vacuum
@04:16:35.59 Cartridge 12 filtering 205/1000ml at 0.33ml/s with 24.5psi vacuum 6.4m deep (24.1psia)
@04:17:35.59 Cartridge 12 filtering 223/1000ml at 0.29ml/s with 25.5psi vacuum 4.5m deep (21.3psia)
@04:18:35.59 Cartridge 12 filtering 240/1000ml at 0.27ml/s with 18.0psi vacuum 3.7m deep (20.1psia)
@04:19:35.59 Cartridge 12 filtering 257/1000ml at 0.23ml/s with 23.1psi vacuum 3.8m deep (20.3psia)
@04:20:35.59 Cartridge 12 filtering 274/1000ml at 0.28ml/s with 18.5psi vacuum 4.7m deep (21.6psia)
@04:21:35.59 Cartridge 12 filtering 290/1000ml at 0.29ml/s with 28.6psi vacuum 3.5m deep (19.8psia)
@04:22:35.59 Cartridge 12 filtering 307/1000ml at 0.26ml/s with 27.7psi vacuum 2.6m deep (18.6psia)
@04:23:35.59 Cartridge 12 filtering 323/1000ml at 0.28ml/s with 20.3psi vacuum 6.3m deep (24.0psia)
@04:24:35.59 Cartridge 12 filtering 339/1000ml at 0.28ml/s with 23.9psi vacuum 3.4m deep (19.8psia)
@04:25:35.59 Cartridge 12 filtering 355/1000ml at 0.30ml/s with 23.0psi vacuum 3.2m deep (19.4psia)
@04:26:35.59 Cartridge 12 filtering 371/1000ml at 0.27ml/s with 25.8psi vacuum 3.3m deep (19.5psia)
@04:27:35.59 Cartridge 12 filtering 386/1000ml at 0.22ml/s with 28.3psi vacuum
@04:28:35.59 Cartridge 12 filtering 402/1000ml at 0.27ml/s with 27.7psi vacuum 4.8m deep (21.7psia)
@04:29:35.59 Cartridge 12 filtering 417/1000ml at 0.22ml/s with 21.1psi vacuum 3.3m deep (19.5psia)
@04:30:35.59 Cartridge 12 filtering 432/1000ml at 0.21ml/s with 27.9psi vacuum 2.3m deep (18.1psia)
@04:31:35.59 Cartridge 12 filtering 447/1000ml at 0.26ml/s with 24.8psi vacuum 3.7m deep (20.2psia)
@04:32:35.59 Cartridge 12 filtering 462/1000ml at 0.22ml/s with 22.5psi vacuum 1.7m deep (17.3psia)
@04:33:35.59 Cartridge 12 filtering 477/1000ml at 0.25ml/s with 23.9psi vacuum 2.3m deep (18.1psia)
@04:34:35.59 Cartridge 12 filtering 491/1000ml at 0.25ml/s with 20.4psi vacuum 1.9m deep (17.4psia)
@04:35:35.59 Cartridge 12 filtering 506/1000ml at 0.20ml/s with 23.6psi vacuum 3.3m deep (19.5psia)
@04:36:35.59 Cartridge 12 filtering 520/1000ml at 0.22ml/s with 22.0psi vacuum 2.7m deep (18.6psia)
@04:37:35.59 Cartridge 12 filtering 534/1000ml at 0.21ml/s with 28.0psi vacuum 2.2m deep (17.9psia)
@04:38:35.59 Cartridge 12 filtering 548/1000ml at 0.22ml/s with 21.4psi vacuum 3.6m deep (20.0psia)
@04:39:35.59 Cartridge 12 filtering 562/1000ml at 0.21ml/s with 22.2psi vacuum 2.3m deep (18.1psia)
@04:40:35.59 Cartridge 12 filtering 576/1000ml at 0.24ml/s with 18.9psi vacuum 5.5m deep (22.8psia)
@04:41:35.59 Cartridge 12 filtering 590/1000ml at 0.26ml/s with 25.4psi vacuum 2.5m deep (18.4psia)
@04:42:35.59 Cartridge 12 filtering 604/1000ml at 0.23ml/s with 24.2psi vacuum 5.1m deep (22.2psia)
@04:43:35.59 Cartridge 12 filtering 617/1000ml at 0.24ml/s with 26.9psi vacuum 3.1m deep (19.3psia)
@04:44:35.59 Cartridge 12 filtering 630/1000ml at 0.21ml/s with 25.0psi vacuum 4.5m deep (21.3psia)
@04:45:35.59 Cartridge 12 filtering 644/1000ml at 0.22ml/s with 23.8psi vacuum 3.5m deep (19.9psia)
@04:46:35.59 Cartridge 12 filtering 657/1000ml at 0.21ml/s with 27.9psi vacuum 2.3m deep (18.1psia)
@04:47:35.59 Cartridge 12 filtering 670/1000ml at 0.21ml/s with 26.5psi vacuum 2.3m deep (18.1psia)
@04:48:35.59 Cartridge 12 filtering 683/1000ml at 0.22ml/s with 22.8psi vacuum 4.1m deep (20.8psia)
@04:49:35.59 Cartridge 12 filtering 696/1000ml at 0.20ml/s with 27.1psi vacuum
@04:50:35.59 Cartridge 12 filtering 709/1000ml at 0.20ml/s with 24.8psi vacuum 3.3m deep (19.6psia)
@04:51:35.59 Cartridge 12 filtering 721/1000ml at 0.24ml/s with 26.6psi vacuum 1.6m deep (17.1psia)
@04:52:35.59 Cartridge 12 filtering 734/1000ml at 0.20ml/s with 22.8psi vacuum 4.5m deep (21.4psia)
@04:53:35.59 Cartridge 12 filtering 747/1000ml at 0.20ml/s with 25.5psi vacuum 1.6m deep (17.1psia)
@04:54:35.59 Cartridge 12 filtering 759/1000ml at 0.20ml/s with 26.8psi vacuum 2.1m deep (17.7psia)
@04:55:35.59 Cartridge 12 filtering 772/1000ml at 0.20ml/s with 24.3psi vacuum 3.5m deep (19.9psia)
@04:56:35.59 Cartridge 12 filtering 784/1000ml at 0.20ml/s with 26.7psi vacuum
@04:57:35.59 Cartridge 12 filtering 796/1000ml at 0.20ml/s with 30.7psi vacuum 3.6m deep (20.1psia)
@04:58:35.59 Cartridge 12 filtering 809/1000ml at 0.21ml/s with 26.0psi vacuum 2.5m deep (18.4psia)
@04:58:56.88 <FILTERING> Flow averaged 0.25ml/s over 53:25
@04:58:56.91 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi
@04:58:57.49 Relieved excess vacuum in .6 seconds
@04:58:57.51 SamplePump.seek 1000ml,16:05
@04:59:03.73 Flow estimate reduced to 0.20ml/s
@04:59:03.75 Waiting up to 12 seconds for vacuum to reduce from 27.4 to 18.0psi
@04:59:04.33 Relieved excess vacuum in .6 seconds
@04:59:04.35 SamplePump.seek 1000ml,15:59
@04:59:13.53 Flow averaged 0.20ml/s over 10 seconds
@04:59:13.55 <closeIntake> Intake.close
@04:59:13.59 <closeExhaust> Exhaust.close
@04:59:13.77 <FILTERING> Toroid.in :hold
@04:59:13.84 Toroid.seek :bypass
@04:59:15.47 Toroid.in :default
@04:59:15.53 Toroid.seek :clear
@04:59:17.15 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@04:59:17.16 Pressure.coast
@04:59:17.34 Sampled  816.2ml
@04:59:19.58 <LRAUV> -> Cmd.startProcessing
@04:59:19.95 <PROCESSING> Plunge.seek :reagent
@04:59:20.79 Sample loop closed. Ready to Deliver Reagents
@04:59:20.80 Archive.seek :sealed
@04:59:25.07 Archive.seek :evac
@04:59:39.00 delay 1:00
@05:00:39.02 Archive.seek :clear
@05:00:56.73 RNAlater soak for 10:00
@05:10:56.75 Archive.seek :tVent
@05:10:59.33 delay 1 second
@05:11:00.35 Archive.seek :evac
@05:11:15.97 delay 10 seconds
@05:11:26.00 Sample preserved and evacuated
@05:11:27.21 <LRAUV> -> Cmd.stop
@05:11:27.35 Carousel.seek 12
@05:11:27.94 Plunge.seek :locked
@05:11:28.89 <Archive> Archive.in :unlocking
@05:11:29.01 Archive.seek :clear
@05:11:29.10 <Air> Air.in :unlocking
@05:11:29.21 Air.seek :clear
@05:11:29.24 <Lysis1> Lysis1.in :unlocking
@05:11:29.40 Lysis1.seek :clear
@05:11:33.05 <LRAUV> Archive.coast
@05:11:33.10 Air.coast
@05:11:33.15 Lysis1.coast
@05:11:33.20 Lysis2.coast
@05:11:33.26 Plunge.coast
@05:11:33.30 SamplePump.coast
@05:11:33.36 Toroid.coast
@05:11:33.40 Carousel.coast
@05:11:33.45 PinPort.coast
@05:11:33.64 Gate.power :motor,:OFF
@05:11:33.66 Gate.power :core,:OFF
@05:11:33.68 Gate.power :analytic1,:OFF
@05:11:33.70 Gate.power :analytic2,:OFF
@05:11:33.72 Gate.power :analytic3,:OFF
@05:11:33.74 Gate.power :main,:OFF
@05:11:33.76 Safely stopped and ready to power off
##-- file closed 2021-04-25T12:26:11.054Z

##-- file opened 2021-04-25T12:26:11.055Z
@05:26:11.50 -> showStatus
@05:26:13.53 -> Cmd.loadCartridge -9
@05:26:13.67 Gate.power :main,:ON,limit to 5000mA
@05:26:13.68 Gate.power :analytic1,:ON,limit to 5000mA
@05:26:13.71 Gate.power :analytic2,:ON,limit to 5000mA
@05:26:13.73 Gate.power :analytic3,:ON,limit to 5000mA
@05:26:13.86 Powering core bus
@05:26:16.15 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@05:26:16.27 <LRAUV> Archive.configure :default
@05:26:16.39 Air.configure :default
@05:26:16.49 Lysis1.configure :default
@05:26:16.60 Lysis2.configure :default
@05:26:16.72 Plunge.configure :default
@05:26:16.84 SamplePump.configure :default
@05:26:16.99 ExtraPump.configure :default
@05:26:17.10 Toroid.configure :default
@05:26:17.22 Carousel.configure :default
@05:26:17.35 Pressure.configure :default
@05:26:17.47 PinPort.configure :default
@05:26:17.53 All elf controllers running firmware version 4.741
@05:26:17.56 Gate.power :motor,:ON,limit to 5000mA
@05:26:17.69 <Archive_home> Archive.in :default
@05:26:18.00 Archive.home.jog 0
@05:26:18.16 <PinPort_home> PinPort.in :default
@05:26:18.24 <Toroid_home> Toroid.in :default
@05:26:18.28 <Lysis2_home> Lysis2.in :default
@05:26:18.31 <Air_home> Air.in :default
@05:26:18.35 <Lysis1_home> Lysis1.in :default
@05:26:18.40 <Plunge_home> Plunge.in :default
@05:26:18.59 <PinPort_home> PinPort.home.jog 0
@05:26:18.63 <Toroid_home> Toroid.home.jog 0
@05:26:18.66 <Lysis2_home> Lysis2.home.jog 0
@05:26:18.68 <Air_home> Air.home.jog 0
@05:26:18.71 <Lysis1_home> Lysis1.home.jog 0
@05:26:18.74 <Plunge_home> Plunge.home.jog 0
@05:26:19.14 <Archive_home> Archive.in :homing
@05:26:19.42 <Toroid_home> Toroid.in :homing
@05:26:19.46 <Plunge_home> Plunge.in :homing
@05:26:19.50 <Lysis1_home> Lysis1.in :homing
@05:26:19.53 <Archive_home> Archive.jog 150
@05:26:19.57 <Air_home> Air.in :homing
@05:26:19.60 <PinPort_home> PinPort.in :homing
@05:26:19.64 <Lysis2_home> Lysis2.in :homing
@05:26:19.83 <Toroid_home> Toroid.jog 624
@05:26:19.86 <Lysis1_home> Lysis1.jog 1416
@05:26:19.96 <PinPort_home> PinPort.jog -108
@05:26:20.01 <Lysis2_home> Lysis2.jog 1431
@05:26:20.04 <Air_home> Air.jog 156
@05:26:20.06 <Plunge_home> Plunge.jog 1579
@05:26:20.29 <Archive_home> Archive.setRawPosition! 50
@05:26:20.34 Archive.seek :home
@05:26:20.42 <PinPort_home> PinPort.setRawPosition! 0
@05:26:20.46 PinPort.seek :home
@05:26:20.55 PinPort.in :default
@05:26:20.65 <Air_home> Air.setRawPosition! 50
@05:26:20.67 <Archive_home> Archive.in :default
@05:26:20.75 <Air_home> Air.seek :home
@05:26:21.04 Air.in :default
@05:26:21.25 <Lysis1_home> Lysis1.setRawPosition! 50
@05:26:21.30 Lysis1.seek :home
@05:26:21.40 <Plunge_home> Plunge.setRawPosition! 50
@05:26:21.42 <Lysis2_home> Lysis2.setRawPosition! 50
@05:26:21.50 <Plunge_home> Plunge.seek :home
@05:26:21.53 <Lysis2_home> Lysis2.seek :home
@05:26:21.68 <Lysis1_home> Lysis1.in :default
@05:26:21.84 <Plunge_home> Plunge.in :default
@05:26:21.92 <Lysis2_home> Lysis2.in :default
@05:26:22.05 <Toroid_home> Toroid.setRawPosition! 0
@05:26:22.10 Toroid.seek :home
@05:26:22.19 Toroid.in :default
@05:26:22.33 <LRAUV> Toroid.seek :clear
@05:26:23.94 Carousel.in :default
@05:26:24.00 Carousel.home.jog 0
@05:26:24.49 Carousel.in :default
@05:26:24.55 Carousel.seek 12
@05:26:24.69 Selecting Cartridge 7
@05:26:24.94 Carousel.seek 7
@05:26:26.23 Lysis1.in :fast
@05:26:26.30 Lysis1.seek :locked
@05:26:28.90 <Plunge> Plunge.seek :locked
@05:26:29.02 <Air> Air.in :fast
@05:26:29.08 Air.seek :locked
@05:26:31.69 <LRAUV> Powering cartridge bus
@05:26:33.81 Gate.setIR 1750,5000,0,6
@05:26:33.94 Gate.setIR 0,5000,0,6
@05:26:34.00 PuckHeater.configure :default
@05:26:34.05 Gate.power :heat,:ON,limit to 5000mA
@05:26:34.71 Mounted lysis Cartridge board#Q05087
@05:26:34.74 Using default puck heat loss curve for HeatingAccelInCarousel
@05:26:53.92 -> Cmd.startFiltering
@05:26:54.37 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@05:26:54.39 SamplePump.setPosition! 0ml
@05:26:54.44 Bypass.open
@05:26:55.93 Intake.open
@05:26:56.73 Sea 3.9m deep (20.5psia)
@05:26:56.74 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@05:26:59.33 Vacuum tare is 51% of full scale
@05:26:59.52 Pressure.in :default
@05:26:59.58 Pressure.hold
@05:26:59.75 SamplePump.in :prime
@05:26:59.82 SamplePump.seek 10ml,1:21
@05:27:09.21 Pressure.coast
@05:27:09.26 SamplePump.setPosition! 0ml
@05:27:09.31 Exhaust.open
@05:27:10.72 <closeBypass> Bypass.close
@05:27:12.26 <FILTERING> Pressure.in :default
@05:27:12.32 Pressure.hold
@05:27:12.43 Sea 3.9m deep (20.5psia)
@05:27:12.56 SamplePump.in :prime
@05:27:12.63 SamplePump.seek 100ml,8:51
@05:29:22.69 Pressure.coast
@05:29:22.75 Bypass.open
@05:29:23.48 Intake.close
@05:29:24.34 Toroid.in :hold
@05:29:24.40 Toroid.seek :crack
@05:29:25.97 Toroid.seek :cartridge
@05:29:27.05 Toroid.in :default
@05:29:27.11 Toroid.seek :clear
@05:29:29.47 Pressurizing cartridge to 26psi above 20.9psia
@05:29:29.48 SamplePump.setPosition! 0ml
@05:29:29.52 Intake.open
@05:29:30.27 Bypass.close
@05:29:31.02 Exhaust.close
@05:29:31.85 Pressure.in :default
@05:29:31.91 Pressure.hold
@05:29:32.09 SamplePump.in :charge
@05:29:32.15 SamplePump.seek 2.3ml,42 seconds
@05:29:42.60 Pressure.coast
@05:29:42.65 Intake.close
@05:29:43.48 Pressurized cartridge with 0.486ml to 26.1psi
@05:29:43.49 Isolated @43.4psia (22.5psi)
@05:29:43.50 Verifying cartridge holds at least 0.75..0.85 of 43.4psia
@05:29:43.53 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@05:29:48.10 Cartridge pressure fell 1% to 42.8psia
@05:29:48.18 Sampling 1000.0ml
@05:29:48.19 SamplePump.setPosition! 0ml
@05:29:48.24 Exhaust.open
@05:29:48.98 Intake.open
@05:29:49.82 Pressure.in :default
@05:29:49.88 Pressure.hold
@05:29:49.99 Sea 6.2m deep (23.8psia)
@05:29:50.12 SamplePump.in :sample
@05:29:50.19 SamplePump.seek 1000ml,1:23:53
@05:29:52.86 <sampling> Cartridge 7 filtering 1/1000ml at 0.34ml/s with 22.3psi vacuum 2.8m deep (18.9psia)
@05:30:52.85 Cartridge 7 filtering 20/1000ml at 0.32ml/s with 27.5psi vacuum 3.8m deep (20.2psia)
@05:31:52.86 Cartridge 7 filtering 39/1000ml at 0.31ml/s with 20.7psi vacuum 2.1m deep (17.8psia)
@05:32:52.85 Cartridge 7 filtering 58/1000ml at 0.34ml/s with 22.2psi vacuum 6.0m deep (23.6psia)
@05:33:52.86 Cartridge 7 filtering 76/1000ml at 0.33ml/s with 26.6psi vacuum 2.5m deep (18.4psia)
@05:34:52.85 Cartridge 7 filtering 94/1000ml at 0.26ml/s with 23.8psi vacuum 2.9m deep (18.9psia)
@05:35:52.86 Cartridge 7 filtering 112/1000ml at 0.32ml/s with 20.6psi vacuum 5.0m deep (22.1psia)
@05:36:52.85 Cartridge 7 filtering 129/1000ml at 0.26ml/s with 22.0psi vacuum 2.3m deep (18.0psia)
@05:37:52.86 Cartridge 7 filtering 147/1000ml at 0.24ml/s with 22.4psi vacuum 4.6m deep (21.4psia)
@05:38:52.85 Cartridge 7 filtering 164/1000ml at 0.28ml/s with 21.4psi vacuum 2.4m deep (18.2psia)
@05:39:52.86 Cartridge 7 filtering 181/1000ml at 0.24ml/s with 26.6psi vacuum 3.5m deep (19.8psia)
@05:40:52.85 Cartridge 7 filtering 197/1000ml at 0.25ml/s with 21.9psi vacuum 3.1m deep (19.3psia)
@05:41:52.86 Cartridge 7 filtering 214/1000ml at 0.32ml/s with 24.1psi vacuum 3.8m deep (20.3psia)
@05:42:52.85 Cartridge 7 filtering 230/1000ml at 0.31ml/s with 25.7psi vacuum 4.1m deep (20.8psia)
@05:43:52.86 Cartridge 7 filtering 247/1000ml at 0.23ml/s with 21.8psi vacuum 1.9m deep (17.5psia)
@05:44:52.85 Cartridge 7 filtering 263/1000ml at 0.22ml/s with 24.2psi vacuum 3.7m deep (20.2psia)
@05:45:52.86 Cartridge 7 filtering 279/1000ml at 0.28ml/s with 20.9psi vacuum 2.3m deep (18.1psia)
@05:46:52.85 Cartridge 7 filtering 294/1000ml at 0.25ml/s with 21.4psi vacuum 4.5m deep (21.3psia)
@05:47:52.86 Cartridge 7 filtering 310/1000ml at 0.29ml/s with 24.9psi vacuum 3.4m deep (19.8psia)
@05:48:52.85 Cartridge 7 filtering 326/1000ml at 0.28ml/s with 21.7psi vacuum 3.7m deep (20.2psia)
@05:49:52.86 Cartridge 7 filtering 341/1000ml at 0.24ml/s with 28.0psi vacuum 3.5m deep (19.9psia)
@05:50:52.85 Cartridge 7 filtering 356/1000ml at 0.28ml/s with 23.1psi vacuum 2.2m deep (17.9psia)
@05:51:52.86 Cartridge 7 filtering 371/1000ml at 0.24ml/s with 27.9psi vacuum 5.0m deep (22.1psia)
@05:52:52.85 Cartridge 7 filtering 386/1000ml at 0.28ml/s with 23.0psi vacuum 2.3m deep (18.1psia)
@05:53:52.86 Cartridge 7 filtering 400/1000ml at 0.21ml/s with 27.3psi vacuum 3.3m deep (19.5psia)
@05:54:52.85 Cartridge 7 filtering 415/1000ml at 0.27ml/s with 25.9psi vacuum 3.2m deep (19.4psia)
@05:55:52.86 Cartridge 7 filtering 429/1000ml at 0.21ml/s with 22.9psi vacuum 2.0m deep (17.6psia)
@05:56:52.85 Cartridge 7 filtering 444/1000ml at 0.21ml/s with 23.2psi vacuum 4.1m deep (20.8psia)
@05:57:52.86 Cartridge 7 filtering 458/1000ml at 0.26ml/s with 22.8psi vacuum 2.3m deep (18.2psia)
@05:58:52.85 Cartridge 7 filtering 472/1000ml at 0.26ml/s with 26.3psi vacuum 4.5m deep (21.3psia)
@05:59:52.86 Cartridge 7 filtering 486/1000ml at 0.25ml/s with 21.5psi vacuum 2.9m deep (18.9psia)
@06:00:52.85 Cartridge 7 filtering 499/1000ml at 0.24ml/s with 25.9psi vacuum 5.1m deep (22.2psia)
@06:01:52.86 Cartridge 7 filtering 513/1000ml at 0.24ml/s with 25.9psi vacuum 4.0m deep (20.6psia)
@06:02:52.85 Cartridge 7 filtering 527/1000ml at 0.23ml/s with 21.6psi vacuum 3.0m deep (19.1psia)
@06:03:52.86 Cartridge 7 filtering 540/1000ml at 0.23ml/s with 21.0psi vacuum 5.4m deep (22.6psia)
@06:04:52.85 Cartridge 7 filtering 553/1000ml at 0.22ml/s with 23.2psi vacuum 2.1m deep (17.8psia)
@06:05:52.86 Cartridge 7 filtering 567/1000ml at 0.20ml/s with 28.8psi vacuum 2.7m deep (18.7psia)
@06:06:52.85 Cartridge 7 filtering 580/1000ml at 0.20ml/s with 21.6psi vacuum 4.3m deep (21.0psia)
@06:07:52.86 Cartridge 7 filtering 593/1000ml at 0.20ml/s with 23.9psi vacuum 1.9m deep (17.4psia)
@06:08:52.85 Cartridge 7 filtering 606/1000ml at 0.20ml/s with 28.3psi vacuum 3.9m deep (20.5psia)
@06:09:52.86 Cartridge 7 filtering 619/1000ml at 0.22ml/s with 23.9psi vacuum
@06:10:52.85 Cartridge 7 filtering 632/1000ml at 0.23ml/s with 23.3psi vacuum 4.6m deep (21.5psia)
@06:11:52.86 Cartridge 7 filtering 644/1000ml at 0.23ml/s with 23.9psi vacuum 2.8m deep (18.9psia)
@06:12:52.85 Cartridge 7 filtering 657/1000ml at 0.21ml/s with 24.3psi vacuum 3.4m deep (19.7psia)
@06:13:52.86 Cartridge 7 filtering 670/1000ml at 0.20ml/s with 28.2psi vacuum 2.8m deep (18.9psia)
@06:14:52.85 Cartridge 7 filtering 682/1000ml at 0.20ml/s with 24.9psi vacuum 1.8m deep (17.4psia)
@06:15:52.86 Cartridge 7 filtering 695/1000ml at 0.20ml/s with 25.8psi vacuum 4.3m deep (21.0psia)
@06:16:52.85 Cartridge 7 filtering 707/1000ml at 0.20ml/s with 27.3psi vacuum
@06:17:52.86 Cartridge 7 filtering 719/1000ml at 0.21ml/s with 24.3psi vacuum 4.2m deep (20.9psia)
@06:18:52.85 Cartridge 7 filtering 732/1000ml at 0.20ml/s with 28.2psi vacuum 2.3m deep (18.0psia)
@06:19:52.86 Cartridge 7 filtering 744/1000ml at 0.20ml/s with 24.6psi vacuum 3.0m deep (19.1psia)
@06:20:52.85 Cartridge 7 filtering 756/1000ml at 0.21ml/s with 23.9psi vacuum 4.8m deep (21.8psia)
@06:21:52.86 Cartridge 7 filtering 768/1000ml at 0.21ml/s with 26.4psi vacuum
@06:22:52.85 Cartridge 7 filtering 781/1000ml at 0.20ml/s with 26.6psi vacuum 5.5m deep (22.8psia)
@06:23:52.86 Cartridge 7 filtering 793/1000ml at 0.20ml/s with 27.8psi vacuum 2.3m deep (18.1psia)
@06:24:51.92 <FILTERING> Flow averaged 0.24ml/s over 55:02
@06:24:51.95 Waiting up to 12 seconds for vacuum to reduce from 27.7 to 18.0psi
@06:24:52.52 Relieved excess vacuum in .6 seconds
@06:24:52.55 SamplePump.seek 1000ml,16:47
@06:24:52.85 <sampling> Cartridge 7 filtering 805/1000ml at 0.07ml/s with 3.9psi vacuum 6.0m deep (23.5psia)
@06:25:52.85 Cartridge 7 filtering 817/1000ml at 0.20ml/s with 27.1psi vacuum 2.9m deep (19.0psia)
@06:26:34.83 <FILTERING> Flow averaged 0.20ml/s over 1:43
@06:26:34.86 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi
@06:26:35.68 Relieved excess vacuum in .9 seconds
@06:26:35.71 SamplePump.seek 1000ml,15:04
@06:26:52.85 <sampling> Cartridge 7 filtering 829/1000ml at 0.20ml/s with 23.2psi vacuum 2.3m deep (18.1psia)
@06:27:13.28 <FILTERING> Flow averaged 0.20ml/s over 38 seconds
@06:27:13.31 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi
@06:27:13.87 Relieved excess vacuum in .6 seconds
@06:27:13.90 SamplePump.seek 1000ml,14:26
@06:27:20.27 Flow estimate reduced to 0.16ml/s
@06:27:20.28 <closeIntake> Intake.close
@06:27:20.33 <closeExhaust> Exhaust.close
@06:27:20.53 <FILTERING> Toroid.in :hold
@06:27:20.60 Toroid.seek :bypass
@06:27:22.23 Toroid.in :default
@06:27:22.29 Toroid.seek :clear
@06:27:23.91 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@06:27:23.92 Pressure.coast
@06:27:24.10 Sampled  834.3ml
@06:27:26.03 <LRAUV> -> Cmd.startProcessing
@06:27:27.88 <PROCESSING> SPR.command \"read_sequences\"
@06:27:27.92 SPR.command \"stats_on\"
@06:27:28.71 SPR.command \"start 0 1\"
@06:27:30.72 <SPRlogger> Begun log /var/log/esp/spr21apr2506h.spr
@06:27:31.55 <PROCESSING> Handoff.select :waste
@06:27:38.23 Syringe tare is 20% of full scale
@06:27:38.25 PinPort.in :locking
@06:27:38.32 PinPort.seek :locked
@06:27:38.80 <posArchive> Archive.in :fast
@06:27:38.86 <posLyS2> Lysis2.in :fast
@06:27:38.89 <PROCESSING> Lysis1.seek 1350uL
@06:27:39.00 <posArchive> Archive.seek :full
@06:27:39.10 <posLyS2> Lysis2.seek :full
@06:27:41.15 <PROCESSING> Plunge.seek :reagent
@06:27:42.05 Evacuating puck with filter bubble point of 50psi
@06:27:42.07 PinPort.seek :locked
@06:27:42.15 Handoff.select :waste
@06:27:48.74 Air syringe pumping up to 1 stroke to reach 8psi
@06:27:48.75 Air stroke #1 of 1
@06:27:48.84 Air.in :pressurize
@06:27:48.90 Air.seek :pressurized
@06:28:00.16 -0.1psi after pressurize
@06:28:00.18 Air.in :compress
@06:28:00.25 Air.seek :evac
@06:28:04.33 0.2psi after compress
@06:28:16.91 0.1psi after compress (stable)
@06:28:16.92 Air.in :default
@06:28:16.99 Air.hold
@06:28:17.16 at 0.1psi Opened to waste
@06:28:17.17 Handoff.select :blocked
@06:28:17.30 Archive syringe pumping up to 5 strokes to reach 50psi
@06:28:17.31 Archive stroke #1 of 5
@06:28:17.38 Archive.in :pressurize
@06:28:17.46 Archive.seek :pressurized
@06:28:30.20 7.1psi after pressurize
@06:28:30.21 Archive.in :compress
@06:28:30.29 Archive.seek :empty
@06:28:46.19 32.3psi after compress
@06:28:59.01 Reached 50.1 of 50.0psi after pumping 1730uL (1.00 strokes)
@06:28:59.04 Archive.in :default
@06:28:59.11 Archive.hold
@06:28:59.18 Handoff.select :waste
@06:29:06.89 delay 1:00
@06:30:06.91 Handoff.select :blocked
@06:30:29.58 Handoff.select :waste
@06:30:29.70 1.5psi at evacEnd
@06:30:29.71 Handoff.select :blocked
@06:30:29.84 Archive.in :fast
@06:30:29.91 Archive.seek :topVent
@06:30:43.61 Stable @2.1psi after Handoff blocked
@06:30:43.63 Archive syringe pumping up to 5 strokes to reach 32psi
@06:30:43.64 Archive stroke #1 of 5
@06:30:43.74 Archive.in :pressurize
@06:30:43.80 Archive.seek :pressurized
@06:30:56.54 3.2psi after pressurize
@06:30:56.56 Archive.in :compress
@06:30:56.63 Archive.seek :empty
@06:31:12.52 7.2psi after compress
@06:31:37.08 Reached 32.2 of 32.1psi after pumping 1730uL (1.00 strokes)
@06:31:37.10 Archive.in :default
@06:31:37.17 Archive.hold
@06:31:37.24 Handoff.select :waste
@06:31:53.92 delay 1:00
@06:32:53.94 Handoff.select :blocked
@06:33:29.63 Handoff.select :waste
@06:33:29.75 6.0psi at evacEnd
@06:33:29.77 Air.seek :fullVent
@06:33:31.21 Archive.in :fast
@06:33:31.27 Archive.seek :topVent
@06:33:38.41 Seawater evacuated, Presure above/below filter released. Lysis begins
@06:33:38.50 Lysis1.in :slow
@06:33:38.56 Lysis1.seek 1050uL
@06:34:08.59 delay 5 seconds
@06:34:13.61 Handoff.select :blocked
@06:34:13.86 Lysis1.seek 450uL
@06:35:13.84 delay 3 seconds
@06:35:16.91 Air.in to mix into 1.37806 parts Lysis1
@06:35:17.41 <Airmixer> Air.seek :evac
@06:35:17.46 <PROCESSING> Lysis1.seek 300uL
@06:35:32.57 Lysis1.seek :empty
@06:36:02.68 Lysis buffer delivered @26.6psi
@06:36:02.69 Polling Can every 2:00
@06:36:03.24 PuckHeater.configure :default #ambient 11.7C
@06:36:03.29 PuckHeater.seek 80.2C
@06:37:14.91 PuckHeater.hold 2:00,80.2C
@06:39:15.08 Polling Can every 2:00
@06:39:15.63 PuckHeater.configure :default #ambient 11.7C
@06:39:15.68 PuckHeater.hold 30 seconds,79.2C
@06:39:45.91 Polling Can every 2:00
@06:39:46.46 PuckHeater.configure :default #ambient 11.7C
@06:39:46.50 PuckHeater.hold 30 seconds,78.6C
@06:40:16.71 Polling Can every 2:00
@06:40:17.26 PuckHeater.configure :default #ambient 11.7C
@06:40:17.31 PuckHeater.hold 10:00,78.1C
@06:50:17.72 Lysis complete. Syringe pressure @25.8psi
@06:50:17.73 Handoff.select :blocked
@06:50:17.78 Archive syringe pumping up to 3 strokes to reach 40psi
@06:50:17.79 Archive stroke #1 of 3
@06:50:17.89 Archive.in :pressurize
@06:50:17.95 Archive.seek :pressurized
@06:50:30.70 25.6psi after pressurize
@06:50:30.71 Archive.in :compress
@06:50:30.79 Archive.seek :empty
@06:50:39.81 Reached 40.8 of 40.0psi after pumping 1583uL (0.92 strokes)
@06:50:39.82 Archive.in :default
@06:50:39.89 Archive.hold
@06:50:39.96 delay 2 seconds
@06:50:41.98 Archive.in :fast
@06:50:42.05 Archive.seek :full
@06:50:48.74 Lysis1.seek 138uL
@06:51:02.63 delay 1 second
@06:51:03.66 <fillArchive> Archive.seek :full
@06:51:03.72 <PROCESSING> Lysis1.seek :empty
@06:51:17.71 Initial air pressure @50.3psi
@06:51:17.78 Lysis1.in :extract
@06:51:17.87 Lysis1.seek 600uL,nil
@06:51:19.58 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@06:51:19.59 Archive stroke #1 of 6
@06:51:19.68 Archive.in :pressurize
@06:51:19.76 Archive.seek :pressurized
@06:51:32.53 16.0psi after pressurize
@06:51:32.56 Archive.in :compress
@06:51:32.63 Archive.seek :empty
@06:51:48.53 15.3psi after compress
@06:52:01.10 15.8psi after compress (stable)
@06:52:06.73 Recovering 2.6uL/s
@06:52:11.80 Recovering 2.2uL/s
@06:52:16.87 Archive.in :gulp
@06:52:16.93 Archive.seek :topVent
@06:52:24.25 16.0psi after gulp
@06:52:36.83 15.8psi after gulp (stable)
@06:52:36.85 Archive stroke #2 of 6
@06:52:36.94 Archive.in :pressurize
@06:52:37.00 Archive.seek :pressurized
@06:52:49.75 15.8psi after pressurize
@06:52:49.76 Archive.in :compress
@06:52:49.83 Archive.seek :empty
@06:53:01.58 Archive.in :default
@06:53:01.63 Archive.hold
@06:53:01.87 <PROCESSING> Initial 600uL lysate @18.8psi
@06:53:01.88 Handoff.select :blocked
@06:53:01.98 Initial air pressure @18.9psi
@06:53:02.05 Lysis1.seek 825uL,nil
@06:53:02.12 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@06:53:02.13 Archive stroke #1 of 6
@06:53:02.27 Archive.in :compress
@06:53:02.34 Archive.seek :empty
@06:53:03.72 Reached 17.4 of 17.0psi after pumping 1671uL (0.97 strokes)
@06:53:03.74 Archive.in :default
@06:53:03.80 Archive.hold
@06:53:04.53 Archive syringe pumping up to 6 strokes to reach 17psi
@06:53:04.54 Archive stroke #1 of 6
@06:53:04.63 Archive.in :compress
@06:53:04.70 Archive.seek :empty
@06:53:05.29 Reached 17.3 of 17.0psi after pumping 1678uL (0.97 strokes)
@06:53:05.30 Archive.in :default
@06:53:05.37 Archive.hold
@06:53:06.10 Archive syringe pumping up to 6 strokes to reach 17psi
@06:53:06.11 Archive stroke #1 of 6
@06:53:06.27 Archive.in :compress
@06:53:06.34 Archive.seek :empty
@06:53:06.72 Reached 16.9 of 17.0psi after pumping 1682uL (0.97 strokes)
@06:53:06.73 Archive.in :default
@06:53:06.80 Archive.hold
@06:53:07.53 Archive syringe pumping up to 6 strokes to reach 17psi
@06:53:07.54 Archive stroke #1 of 6
@06:53:07.63 Archive.in :compress
@06:53:07.70 Archive.seek :empty
@06:53:10.18 15.6psi after compress
@06:53:19.07 Archive.in :default
@06:53:19.12 Archive.hold
@06:53:19.30 <PROCESSING> Recovered 825uL lysate @16.5psi
@06:53:19.31 Air.in :halfSpeed
@06:53:19.39 Air.seek :fullVent
@06:53:21.65 Puck Top Vented, bottom @13.3psi
@06:53:21.66 Handoff.select :waste
@06:53:21.78 delay 5 seconds
@06:53:26.84 Handoff open to waste, bottom @0.1psi
@06:53:26.86 Air.in :default
@06:53:26.92 Air.seek :evac
@06:53:28.37 Lysis2.in :DAmix
@06:53:28.47 Lysis1.in :DAmix
@06:53:29.00 <Lysis2mixer> Lysis2.seek 1480uL
@06:53:29.04 <PROCESSING> Lysis1.seek 575uL
@06:54:04.11 delay 5 seconds
@06:54:09.13 Handoff.select SPR
@06:54:09.24 SPR.command \"start 2 14\"
@06:54:10.11 delay 10 seconds
@06:54:20.14 SPR.command \"start 1 0\"
@06:54:23.38 Waiting up to 4:00 for SPR to enter state 1
@06:56:25.74 SPR.command \"hold_on\"
@06:56:27.64 SPR Dilution 1 of 1 -- Running Experiment 1
@06:56:28.06 <Lysis2mixer> Lysis2.seek 1180uL
@06:56:28.10 <PROCESSING> Lysis1.seek 275uL
@06:57:10.16 volL1 = 300.0 and volL2 = 300.0
@06:57:10.18 delay 15 seconds
@06:57:25.21 SPR.command \"hold_off\"
@06:57:27.80 Waiting up to 38:00 for SPR to enter state 7
@07:24:10.41 SPR Analysis has Completed
@07:24:10.43 Handoff.select :waste
@07:24:11.34 <SPRlogger> Ended log /var/log/esp/spr21apr2506h.spr
@07:24:11.36 <PROCESSING> SPR.command \"stats_off\"
@07:24:13.09 <LRAUV> -> Cmd.stop
@07:24:13.18 <closeHandoff> Handoff.select :close
@07:24:13.30 <LRAUV> Gate.power :heat,:OFF
@07:24:13.32 Gate.power :cartridge,:OFF
@07:24:13.40 Carousel.seek 7
@07:24:13.58 Plunge.seek :locked
@07:24:14.46 PinPort.in :unlocking
@07:24:14.52 PinPort.seek :clear
@07:24:15.06 <Archive> Archive.in :unlocking
@07:24:15.19 Archive.seek :clear
@07:24:15.25 <Air> Air.in :unlocking
@07:24:15.39 Air.seek :clear
@07:24:15.42 <Lysis1> Lysis1.in :unlocking
@07:24:15.58 Lysis1.seek :clear
@07:24:15.65 <Lysis2> Lysis2.in :unlocking
@07:24:15.72 Lysis2.seek :clear
@07:24:19.25 <LRAUV> Archive.coast
@07:24:19.30 Air.coast
@07:24:19.35 Lysis1.coast
@07:24:19.41 Lysis2.coast
@07:24:19.46 Plunge.coast
@07:24:19.51 SamplePump.coast
@07:24:19.56 Toroid.coast
@07:24:19.61 Carousel.coast
@07:24:19.66 PinPort.coast
@07:24:19.85 Gate.power :motor,:OFF
@07:24:19.87 Gate.power :core,:OFF
@07:24:19.89 Gate.power :analytic1,:OFF
@07:24:19.91 Gate.power :analytic2,:OFF
@07:24:19.93 Gate.power :analytic3,:OFF
@07:24:19.95 Gate.power :main,:OFF
@07:24:19.97 Safely stopped and ready to power off
##-- file closed 2021-04-25T16:31:21.124Z

##-- file opened 2021-04-25T16:31:21.126Z
@09:31:21.37 -> showStatus
@09:31:23.39 -> Cmd.loadCartridge -6
@09:31:23.53 Gate.power :main,:ON,limit to 5000mA
@09:31:23.54 Gate.power :analytic1,:ON,limit to 5000mA
@09:31:23.56 Gate.power :analytic2,:ON,limit to 5000mA
@09:31:23.59 Gate.power :analytic3,:ON,limit to 5000mA
@09:31:23.72 Powering core bus
@09:31:26.01 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@09:31:26.13 <LRAUV> Archive.configure :default
@09:31:26.25 Air.configure :default
@09:31:26.35 Lysis1.configure :default
@09:31:26.46 Lysis2.configure :default
@09:31:26.59 Plunge.configure :default
@09:31:26.71 SamplePump.configure :default
@09:31:26.86 ExtraPump.configure :default
@09:31:26.98 Toroid.configure :default
@09:31:27.10 Carousel.configure :default
@09:31:27.22 Pressure.configure :default
@09:31:27.34 PinPort.configure :default
@09:31:27.40 All elf controllers running firmware version 4.741
@09:31:27.43 Gate.power :motor,:ON,limit to 5000mA
@09:31:27.60 <Archive_home> Archive.in :default
@09:31:27.88 Archive.home.jog 0
@09:31:28.04 <Toroid_home> Toroid.in :default
@09:31:28.12 <Plunge_home> Plunge.in :default
@09:31:28.16 <Lysis1_home> Lysis1.in :default
@09:31:28.19 <PinPort_home> PinPort.in :default
@09:31:28.27 <Air_home> Air.in :default
@09:31:28.31 <Lysis2_home> Lysis2.in :default
@09:31:28.45 <Toroid_home> Toroid.home.jog 0
@09:31:28.50 <Plunge_home> Plunge.home.jog 0
@09:31:28.56 <Lysis1_home> Lysis1.home.jog 0
@09:31:28.64 <PinPort_home> PinPort.home.jog 0
@09:31:28.68 <Air_home> Air.home.jog 0
@09:31:28.71 <Lysis2_home> Lysis2.home.jog 0
@09:31:29.02 <Archive_home> Archive.in :homing
@09:31:29.23 <Toroid_home> Toroid.in :homing
@09:31:29.26 <Plunge_home> Plunge.in :homing
@09:31:29.31 <Archive_home> Archive.jog 194
@09:31:29.36 <Lysis1_home> Lysis1.in :homing
@09:31:29.39 <PinPort_home> PinPort.in :homing
@09:31:29.56 <Toroid_home> Toroid.jog 616
@09:31:29.59 <Plunge_home> Plunge.jog 1584
@09:31:29.69 <PinPort_home> PinPort.jog -108
@09:31:29.75 <Lysis1_home> Lysis1.jog 1415
@09:31:29.90 <Air_home> Air.in :homing
@09:31:29.92 <Lysis2_home> Lysis2.in :homing
@09:31:30.01 <Air_home> Air.jog 166
@09:31:30.04 <Lysis2_home> Lysis2.jog 1429
@09:31:30.16 <Archive_home> Archive.setRawPosition! 50
@09:31:30.18 <PinPort_home> PinPort.setRawPosition! 0
@09:31:30.25 <Archive_home> Archive.seek :home
@09:31:30.28 <PinPort_home> PinPort.seek :home
@09:31:30.41 PinPort.in :default
@09:31:30.50 <Air_home> Air.setRawPosition! 50
@09:31:30.54 Air.seek :home
@09:31:30.64 <Archive_home> Archive.in :default
@09:31:30.83 <Air_home> Air.in :default
@09:31:30.92 <Plunge_home> Plunge.setRawPosition! 50
@09:31:30.97 Plunge.seek :home
@09:31:31.05 <Lysis1_home> Lysis1.setRawPosition! 50
@09:31:31.10 Lysis1.seek :home
@09:31:31.26 <Lysis2_home> Lysis2.setRawPosition! 50
@09:31:31.31 Lysis2.seek :home
@09:31:31.40 <Plunge_home> Plunge.in :default
@09:31:31.43 <Lysis1_home> Lysis1.in :default
@09:31:31.59 <Lysis2_home> Lysis2.in :default
@09:31:31.74 <Toroid_home> Toroid.setRawPosition! 0
@09:31:31.79 Toroid.seek :home
@09:31:31.88 Toroid.in :default
@09:31:32.02 <LRAUV> Toroid.seek :clear
@09:31:33.64 Carousel.in :default
@09:31:33.70 Carousel.home.jog 0
@09:31:34.20 Carousel.in :default
@09:31:34.26 Carousel.seek 7
@09:31:34.42 Selecting Cartridge 10
@09:31:34.66 Carousel.seek 10
@09:31:35.55 Lysis1.in :fast
@09:31:35.62 Lysis1.seek :locked
@09:31:38.21 <Plunge> Plunge.seek :locked
@09:31:38.33 <Air> Air.in :fast
@09:31:38.40 Air.seek :locked
@09:31:41.00 <LRAUV> Powering cartridge bus
@09:31:43.12 Gate.setIR 1750,5000,0,6
@09:31:43.25 Gate.setIR 0,5000,0,6
@09:31:43.30 Gate.power :cartridge,:OFF
@09:31:43.83 Mounted archive Cartridge
@09:32:02.98 -> Cmd.startFiltering
@09:32:03.43 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@09:32:03.45 SamplePump.setPosition! 0ml
@09:32:03.49 Bypass.open
@09:32:04.98 Intake.open
@09:32:05.76 Sea 5.2m deep (22.4psia)
@09:32:05.77 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@09:32:08.34 Vacuum tare is 51% of full scale
@09:32:08.52 Pressure.in :default
@09:32:08.59 Pressure.hold
@09:32:08.76 SamplePump.in :prime
@09:32:08.82 SamplePump.seek 10ml,1:21
@09:32:18.21 Pressure.coast
@09:32:18.26 SamplePump.setPosition! 0ml
@09:32:18.31 Exhaust.open
@09:32:19.72 <closeBypass> Bypass.close
@09:32:21.26 <FILTERING> Pressure.in :default
@09:32:21.32 Pressure.hold
@09:32:21.43 Sea 4.2m deep (20.9psia)
@09:32:21.56 SamplePump.in :prime
@09:32:21.63 SamplePump.seek 100ml,8:51
@09:34:31.87 Pressure.coast
@09:34:31.93 Bypass.open
@09:34:32.10 Intake.close
@09:34:32.94 Toroid.in :hold
@09:34:33.00 Toroid.seek :crack
@09:34:34.57 Toroid.seek :cartridge
@09:34:35.65 Toroid.in :default
@09:34:35.71 Toroid.seek :clear
@09:34:38.07 Pressurizing cartridge to 26psi above 21.4psia
@09:34:38.08 SamplePump.setPosition! 0ml
@09:34:38.12 Intake.open
@09:34:38.86 Bypass.close
@09:34:39.58 Exhaust.close
@09:34:40.36 Pressure.in :default
@09:34:40.42 Pressure.hold
@09:34:40.60 SamplePump.in :charge
@09:34:40.66 SamplePump.seek 2.3ml,42 seconds
@09:34:47.20 Pressure.coast
@09:34:47.25 Intake.close
@09:34:48.06 Pressurized cartridge with 0.188ml to 26.5psi
@09:34:48.07 Isolated @42.3psia (21.0psi)
@09:34:48.08 Verifying cartridge holds at least 0.75..0.85 of 42.3psia
@09:34:48.11 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@09:34:52.68 Cartridge pressure fell 1% to 42.1psia
@09:34:52.76 Sampling 1000.0ml
@09:34:52.77 SamplePump.setPosition! 0ml
@09:34:52.82 Exhaust.open
@09:34:53.53 Intake.open
@09:34:54.37 Pressure.in :default
@09:34:54.43 Pressure.hold
@09:34:54.55 Sea 4.0m deep (20.6psia)
@09:34:54.68 SamplePump.in :sample
@09:34:54.75 SamplePump.seek 1000ml,1:23:53
@09:34:57.40 <sampling> Cartridge 10 filtering 1/1000ml at 0.30ml/s with 22.8psi vacuum 1.9m deep (17.5psia)
@09:35:57.41 Cartridge 10 filtering 20/1000ml at 0.35ml/s with 21.0psi vacuum 5.5m deep (22.8psia)
@09:36:57.41 Cartridge 10 filtering 39/1000ml at 0.34ml/s with 20.1psi vacuum 3.4m deep (19.7psia)
@09:37:57.41 Cartridge 10 filtering 58/1000ml at 0.33ml/s with 20.6psi vacuum 3.8m deep (20.3psia)
@09:38:57.41 Cartridge 10 filtering 77/1000ml at 0.31ml/s with 30.2psi vacuum 2.9m deep (19.0psia)
@09:39:57.41 Cartridge 10 filtering 95/1000ml at 0.34ml/s with 21.2psi vacuum 2.9m deep (19.0psia)
@09:40:57.41 Cartridge 10 filtering 113/1000ml at 0.33ml/s with 27.0psi vacuum 4.2m deep (20.9psia)
@09:41:57.41 Cartridge 10 filtering 132/1000ml at 0.34ml/s with 21.8psi vacuum 3.6m deep (20.0psia)
@09:42:57.41 Cartridge 10 filtering 150/1000ml at 0.34ml/s with 25.0psi vacuum 4.3m deep (21.1psia)
@09:43:57.41 Cartridge 10 filtering 167/1000ml at 0.33ml/s with 23.5psi vacuum 5.6m deep (23.0psia)
@09:44:57.41 Cartridge 10 filtering 185/1000ml at 0.25ml/s with 25.5psi vacuum
@09:45:57.41 Cartridge 10 filtering 203/1000ml at 0.24ml/s with 23.6psi vacuum 4.5m deep (21.3psia)
@09:46:57.41 Cartridge 10 filtering 220/1000ml at 0.30ml/s with 26.1psi vacuum 2.6m deep (18.5psia)
@09:47:57.41 Cartridge 10 filtering 238/1000ml at 0.24ml/s with 27.5psi vacuum 3.2m deep (19.4psia)
@09:48:57.41 Cartridge 10 filtering 255/1000ml at 0.23ml/s with 22.8psi vacuum 2.6m deep (18.5psia)
@09:49:57.41 Cartridge 10 filtering 272/1000ml at 0.31ml/s with 22.0psi vacuum 3.2m deep (19.4psia)
@09:50:57.41 Cartridge 10 filtering 289/1000ml at 0.31ml/s with 26.7psi vacuum 4.2m deep (20.8psia)
@09:51:57.41 Cartridge 10 filtering 306/1000ml at 0.32ml/s with 23.5psi vacuum 3.2m deep (19.4psia)
@09:52:57.41 Cartridge 10 filtering 323/1000ml at 0.23ml/s with 21.4psi vacuum 4.2m deep (20.9psia)
@09:53:57.41 Cartridge 10 filtering 340/1000ml at 0.28ml/s with 21.0psi vacuum 3.2m deep (19.5psia)
@09:54:57.41 Cartridge 10 filtering 357/1000ml at 0.24ml/s with 21.7psi vacuum 3.1m deep (19.3psia)
@09:55:57.41 Cartridge 10 filtering 373/1000ml at 0.31ml/s with 22.7psi vacuum 4.7m deep (21.7psia)
@09:56:57.41 Cartridge 10 filtering 390/1000ml at 0.23ml/s with 28.1psi vacuum
@09:57:57.41 Cartridge 10 filtering 406/1000ml at 0.25ml/s with 28.5psi vacuum 4.2m deep (20.9psia)
@09:58:57.41 Cartridge 10 filtering 422/1000ml at 0.31ml/s with 24.3psi vacuum 3.7m deep (20.1psia)
@09:59:57.41 Cartridge 10 filtering 439/1000ml at 0.22ml/s with 21.7psi vacuum 4.5m deep (21.3psia)
@10:00:57.41 Cartridge 10 filtering 455/1000ml at 0.23ml/s with 21.0psi vacuum 2.7m deep (18.7psia)
@10:01:57.41 Cartridge 10 filtering 471/1000ml at 0.22ml/s with 27.4psi vacuum
@10:02:57.41 Cartridge 10 filtering 487/1000ml at 0.29ml/s with 23.0psi vacuum 5.7m deep (23.1psia)
@10:03:57.41 Cartridge 10 filtering 503/1000ml at 0.29ml/s with 22.2psi vacuum 2.6m deep (18.6psia)
@10:04:57.41 Cartridge 10 filtering 518/1000ml at 0.28ml/s with 23.4psi vacuum 5.8m deep (23.2psia)
@10:05:57.41 Cartridge 10 filtering 534/1000ml at 0.23ml/s with 21.0psi vacuum 2.9m deep (18.9psia)
@10:06:57.41 Cartridge 10 filtering 550/1000ml at 0.28ml/s with 21.5psi vacuum 3.3m deep (19.5psia)
@10:07:57.41 Cartridge 10 filtering 565/1000ml at 0.27ml/s with 21.1psi vacuum 5.7m deep (23.1psia)
@10:08:57.41 Cartridge 10 filtering 581/1000ml at 0.27ml/s with 22.2psi vacuum 1.9m deep (17.6psia)
@10:09:57.41 Cartridge 10 filtering 596/1000ml at 0.29ml/s with 24.7psi vacuum 6.5m deep (24.3psia)
@10:10:57.41 Cartridge 10 filtering 611/1000ml at 0.23ml/s with 28.0psi vacuum 2.3m deep (18.1psia)
@10:11:57.41 Cartridge 10 filtering 626/1000ml at 0.28ml/s with 23.2psi vacuum 4.9m deep (21.9psia)
@10:12:57.41 Cartridge 10 filtering 641/1000ml at 0.26ml/s with 22.2psi vacuum 4.4m deep (21.3psia)
@10:13:57.41 Cartridge 10 filtering 656/1000ml at 0.27ml/s with 22.0psi vacuum 3.0m deep (19.1psia)
@10:14:57.41 Cartridge 10 filtering 671/1000ml at 0.26ml/s with 24.7psi vacuum 3.2m deep (19.4psia)
@10:15:57.41 Cartridge 10 filtering 686/1000ml at 0.21ml/s with 22.3psi vacuum
@10:16:57.41 Cartridge 10 filtering 701/1000ml at 0.26ml/s with 23.2psi vacuum 5.5m deep (22.8psia)
@10:17:57.41 Cartridge 10 filtering 715/1000ml at 0.26ml/s with 24.9psi vacuum 2.2m deep (18.0psia)
@10:18:57.41 Cartridge 10 filtering 730/1000ml at 0.22ml/s with 21.2psi vacuum 4.0m deep (20.6psia)
@10:19:57.41 Cartridge 10 filtering 744/1000ml at 0.27ml/s with 23.5psi vacuum 4.3m deep (21.0psia)
@10:20:57.41 Cartridge 10 filtering 759/1000ml at 0.25ml/s with 27.4psi vacuum 1.7m deep (17.2psia)
@10:21:57.41 Cartridge 10 filtering 773/1000ml at 0.27ml/s with 25.1psi vacuum 5.6m deep (23.0psia)
@10:22:57.41 Cartridge 10 filtering 787/1000ml at 0.25ml/s with 23.5psi vacuum 2.4m deep (18.3psia)
@10:23:57.41 Cartridge 10 filtering 801/1000ml at 0.24ml/s with 27.3psi vacuum 3.3m deep (19.5psia)
@10:24:57.41 Cartridge 10 filtering 815/1000ml at 0.20ml/s with 27.1psi vacuum 2.0m deep (17.6psia)
@10:25:57.41 Cartridge 10 filtering 829/1000ml at 0.22ml/s with 26.7psi vacuum 3.0m deep (19.2psia)
@10:26:57.41 Cartridge 10 filtering 843/1000ml at 0.24ml/s with 21.5psi vacuum 4.5m deep (21.3psia)
@10:27:57.45 Cartridge 10 filtering 857/1000ml at 0.21ml/s with 22.4psi vacuum
@10:28:57.41 Cartridge 10 filtering 871/1000ml at 0.25ml/s with 23.8psi vacuum 6.0m deep (23.6psia)
@10:29:57.41 Cartridge 10 filtering 884/1000ml at 0.24ml/s with 25.5psi vacuum 1.6m deep (17.0psia)
@10:30:57.41 Cartridge 10 filtering 898/1000ml at 0.24ml/s with 24.5psi vacuum 4.1m deep (20.8psia)
@10:31:57.41 Cartridge 10 filtering 912/1000ml at 0.20ml/s with 26.0psi vacuum 2.6m deep (18.5psia)
@10:32:57.41 Cartridge 10 filtering 925/1000ml at 0.23ml/s with 25.1psi vacuum 2.6m deep (18.5psia)
@10:33:57.41 Cartridge 10 filtering 939/1000ml at 0.25ml/s with 25.2psi vacuum 5.4m deep (22.6psia)
@10:34:57.41 Cartridge 10 filtering 952/1000ml at 0.22ml/s with 26.9psi vacuum
@10:35:57.41 Cartridge 10 filtering 965/1000ml at 0.25ml/s with 22.1psi vacuum 6.2m deep (23.8psia)
@10:36:57.41 Cartridge 10 filtering 979/1000ml at 0.22ml/s with 22.6psi vacuum 2.6m deep (18.6psia)
@10:37:57.40 Cartridge 10 filtering 992/1000ml at 0.25ml/s with 24.8psi vacuum 4.5m deep (21.4psia)
@10:38:34.99 <FILTERING> Pressure.coast
@10:38:35.11 <closeIntake> Intake.close
@10:38:35.16 <closeExhaust> Exhaust.close
@10:38:35.30 <FILTERING> Toroid.in :hold
@10:38:35.36 Toroid.seek :bypass
@10:38:36.98 Toroid.in :default
@10:38:37.04 Toroid.seek :clear
@10:38:38.66 Sampled  1000.0ml
@10:38:39.96 <LRAUV> -> Cmd.startProcessing
@10:38:40.34 <PROCESSING> Plunge.seek :reagent
@10:38:41.18 Sample loop closed. Ready to Deliver Reagents
@10:38:41.19 Archive.seek :sealed
@10:38:45.45 Archive.seek :evac
@10:38:59.39 delay 1:00
@10:39:59.41 Archive.seek :clear
@10:40:17.12 RNAlater soak for 10:00
@10:50:17.14 Archive.seek :tVent
@10:50:19.72 delay 1 second
@10:50:20.74 Archive.seek :evac
@10:50:36.77 delay 10 seconds
@10:50:46.80 Sample preserved and evacuated
@10:50:48.00 <LRAUV> -> Cmd.stop
@10:50:48.14 Carousel.seek 10
@10:50:48.32 Plunge.seek :locked
@10:50:49.27 <Archive> Archive.in :unlocking
@10:50:49.39 Archive.seek :clear
@10:50:49.46 <Air> Air.in :unlocking
@10:50:49.58 Air.seek :clear
@10:50:49.62 <Lysis1> Lysis1.in :unlocking
@10:50:49.73 Lysis1.seek :clear
@10:50:53.43 <LRAUV> Archive.coast
@10:50:53.49 Air.coast
@10:50:53.53 Lysis1.coast
@10:50:53.59 Lysis2.coast
@10:50:53.64 Plunge.coast
@10:50:53.69 SamplePump.coast
@10:50:53.73 Toroid.coast
@10:50:53.78 Carousel.coast
@10:50:53.84 PinPort.coast
@10:50:54.02 Gate.power :motor,:OFF
@10:50:54.04 Gate.power :core,:OFF
@10:50:54.06 Gate.power :analytic1,:OFF
@10:50:54.08 Gate.power :analytic2,:OFF
@10:50:54.10 Gate.power :analytic3,:OFF
@10:50:54.12 Gate.power :main,:OFF
@10:50:54.14 Safely stopped and ready to power off
##-- file closed 2021-04-25T18:02:52.473Z

##-- file opened 2021-04-25T18:02:52.475Z
@11:02:52.53 -> showStatus
@11:02:54.55 -> Cmd.loadCartridge -9
@11:02:54.69 Gate.power :main,:ON,limit to 5000mA
@11:02:54.70 Gate.power :analytic1,:ON,limit to 5000mA
@11:02:54.72 Gate.power :analytic2,:ON,limit to 5000mA
@11:02:54.75 Gate.power :analytic3,:ON,limit to 5000mA
@11:02:54.88 Powering core bus
@11:02:57.17 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@11:02:57.30 <LRAUV> Archive.configure :default
@11:02:57.42 Air.configure :default
@11:02:57.53 Lysis1.configure :default
@11:02:57.63 Lysis2.configure :default
@11:02:57.75 Plunge.configure :default
@11:02:57.87 SamplePump.configure :default
@11:02:58.02 ExtraPump.configure :default
@11:02:58.13 Toroid.configure :default
@11:02:58.26 Carousel.configure :default
@11:02:58.39 Pressure.configure :default
@11:02:58.50 PinPort.configure :default
@11:02:58.56 All elf controllers running firmware version 4.741
@11:02:58.59 Gate.power :motor,:ON,limit to 5000mA
@11:02:58.76 <Archive_home> Archive.in :default
@11:02:59.11 <PinPort_home> PinPort.in :default
@11:02:59.15 <Toroid_home> Toroid.in :default
@11:02:59.18 <Plunge_home> Plunge.in :default
@11:02:59.21 <Lysis1_home> Lysis1.in :default
@11:02:59.25 <Archive_home> Archive.home.jog 0
@11:02:59.28 <Air_home> Air.in :default
@11:02:59.31 <Lysis2_home> Lysis2.in :default
@11:02:59.50 <PinPort_home> PinPort.home.jog 0
@11:02:59.52 <Toroid_home> Toroid.home.jog 0
@11:02:59.57 <Plunge_home> Plunge.home.jog 0
@11:02:59.60 <Lysis1_home> Lysis1.home.jog 0
@11:02:59.64 <Lysis2_home> Lysis2.home.jog 0
@11:02:59.72 <Air_home> Air.home.jog 0
@11:03:00.19 <Toroid_home> Toroid.in :homing
@11:03:00.23 <Plunge_home> Plunge.in :homing
@11:03:00.28 <PinPort_home> PinPort.in :homing
@11:03:00.34 <Lysis1_home> Lysis1.in :homing
@11:03:00.40 <Archive_home> Archive.in :homing
@11:03:00.56 <Plunge_home> Plunge.jog 1577
@11:03:00.59 <Toroid_home> Toroid.jog 619
@11:03:00.61 <PinPort_home> PinPort.jog -108
@11:03:00.64 <Archive_home> Archive.jog 150
@11:03:00.67 <Lysis1_home> Lysis1.jog 1415
@11:03:00.92 <Air_home> Air.in :homing
@11:03:00.96 <Lysis2_home> Lysis2.in :homing
@11:03:01.04 <Air_home> Air.jog 158
@11:03:01.07 <Lysis2_home> Lysis2.jog 1431
@11:03:01.20 <PinPort_home> PinPort.setRawPosition! 0
@11:03:01.24 PinPort.seek :home
@11:03:01.33 <Archive_home> Archive.setRawPosition! 50
@11:03:01.35 <PinPort_home> PinPort.in :default
@11:03:01.42 <Archive_home> Archive.seek :home
@11:03:01.52 <Air_home> Air.setRawPosition! 50
@11:03:01.57 Air.seek :home
@11:03:01.72 <Archive_home> Archive.in :default
@11:03:01.85 <Air_home> Air.in :default
@11:03:01.92 <Plunge_home> Plunge.setRawPosition! 50
@11:03:01.98 Plunge.seek :home
@11:03:02.06 <Lysis1_home> Lysis1.setRawPosition! 50
@11:03:02.10 Lysis1.seek :home
@11:03:02.28 <Plunge_home> Plunge.in :default
@11:03:02.35 <Lysis2_home> Lysis2.setRawPosition! 50
@11:03:02.41 Lysis2.seek :home
@11:03:02.49 <Lysis1_home> Lysis1.in :default
@11:03:02.69 <Lysis2_home> Lysis2.in :default
@11:03:02.81 <Toroid_home> Toroid.setRawPosition! 0
@11:03:02.85 Toroid.seek :home
@11:03:02.95 Toroid.in :default
@11:03:03.08 <LRAUV> Toroid.seek :clear
@11:03:04.70 Carousel.in :default
@11:03:04.76 Carousel.home.jog 0
@11:03:05.25 Carousel.in :default
@11:03:05.31 Carousel.seek 10
@11:03:05.47 Selecting Cartridge 6
@11:03:05.71 Carousel.seek 6
@11:03:06.80 Lysis1.in :fast
@11:03:06.86 Lysis1.seek :locked
@11:03:09.46 <Plunge> Plunge.seek :locked
@11:03:09.59 <Air> Air.in :fast
@11:03:09.65 Air.seek :locked
@11:03:12.25 <LRAUV> Powering cartridge bus
@11:03:14.37 Gate.setIR 1750,5000,0,6
@11:03:14.51 Gate.setIR 0,5000,0,6
@11:03:14.57 PuckHeater.configure :default
@11:03:14.62 Gate.power :heat,:ON,limit to 5000mA
@11:03:15.36 Mounted lysis Cartridge board#Q05089
@11:03:15.40 Using default puck heat loss curve for HeatingAccelInCarousel
@11:03:34.95 -> Cmd.startFiltering
@11:03:35.40 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@11:03:35.42 SamplePump.setPosition! 0ml
@11:03:35.46 Bypass.open
@11:03:36.93 Intake.open
@11:03:37.76 Sea 6.4m deep (24.2psia)
@11:03:37.77 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@11:03:40.36 Vacuum tare is 51% of full scale
@11:03:40.55 Pressure.in :default
@11:03:40.62 Pressure.hold
@11:03:40.79 SamplePump.in :prime
@11:03:40.85 SamplePump.seek 10ml,1:21
@11:03:50.25 Pressure.coast
@11:03:50.30 SamplePump.setPosition! 0ml
@11:03:50.35 Exhaust.open
@11:03:51.79 <closeBypass> Bypass.close
@11:03:53.33 <FILTERING> Pressure.in :default
@11:03:53.39 Pressure.hold
@11:03:53.50 Sea 5.5m deep (22.8psia)
@11:03:53.63 SamplePump.in :prime
@11:03:53.70 SamplePump.seek 100ml,8:51
@11:06:04.03 Pressure.coast
@11:06:04.09 Bypass.open
@11:06:04.78 Intake.close
@11:06:05.67 Toroid.in :hold
@11:06:05.73 Toroid.seek :crack
@11:06:07.28 Toroid.seek :cartridge
@11:06:08.77 Toroid.in :default
@11:06:08.83 Toroid.seek :clear
@11:06:10.79 Pressurizing cartridge to 26psi above 23.7psia
@11:06:10.80 SamplePump.setPosition! 0ml
@11:06:10.85 Intake.open
@11:06:11.59 Bypass.close
@11:06:12.31 Exhaust.close
@11:06:13.10 Pressure.in :default
@11:06:13.16 Pressure.hold
@11:06:13.33 SamplePump.in :charge
@11:06:13.40 SamplePump.seek 2.3ml,42 seconds
@11:06:22.50 Pressure.coast
@11:06:22.54 Intake.close
@11:06:23.36 Pressurized cartridge with 0.367ml to 26.0psi
@11:06:23.37 Isolated @44.4psia (20.7psi)
@11:06:23.38 Verifying cartridge holds at least 0.75..0.85 of 44.4psia
@11:06:23.41 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@11:06:27.98 Cartridge pressure fell 1% to 44.0psia
@11:06:28.06 Sampling 1000.0ml
@11:06:28.07 SamplePump.setPosition! 0ml
@11:06:28.13 Exhaust.open
@11:06:28.81 Intake.open
@11:06:29.67 Pressure.in :default
@11:06:29.73 Pressure.hold
@11:06:29.84 Sea 6.7m deep (24.6psia)
@11:06:29.98 SamplePump.in :sample
@11:06:30.04 SamplePump.seek 1000ml,1:23:53
@11:06:32.71 <sampling> Cartridge 6 filtering 1/1000ml at 0.32ml/s with 19.5psi vacuum 3.2m deep (19.4psia)
@11:07:32.70 Cartridge 6 filtering 20/1000ml at 0.34ml/s with 22.9psi vacuum 3.8m deep (20.4psia)
@11:08:32.71 Cartridge 6 filtering 39/1000ml at 0.35ml/s with 21.3psi vacuum 4.7m deep (21.7psia)
@11:09:32.70 Cartridge 6 filtering 57/1000ml at 0.33ml/s with 20.8psi vacuum 2.7m deep (18.7psia)
@11:10:32.71 Cartridge 6 filtering 76/1000ml at 0.34ml/s with 22.2psi vacuum 5.3m deep (22.6psia)
@11:11:32.70 Cartridge 6 filtering 94/1000ml at 0.28ml/s with 28.9psi vacuum
@11:12:32.71 Cartridge 6 filtering 111/1000ml at 0.33ml/s with 21.2psi vacuum 4.5m deep (21.4psia)
@11:13:32.70 Cartridge 6 filtering 129/1000ml at 0.33ml/s with 22.3psi vacuum 4.6m deep (21.5psia)
@11:14:32.71 Cartridge 6 filtering 147/1000ml at 0.26ml/s with 22.3psi vacuum
@11:15:32.70 Cartridge 6 filtering 164/1000ml at 0.27ml/s with 20.3psi vacuum 5.8m deep (23.2psia)
@11:16:32.71 Cartridge 6 filtering 181/1000ml at 0.31ml/s with 26.4psi vacuum 2.1m deep (17.8psia)
@11:17:32.70 Cartridge 6 filtering 198/1000ml at 0.26ml/s with 27.6psi vacuum 4.5m deep (21.3psia)
@11:18:32.71 Cartridge 6 filtering 215/1000ml at 0.32ml/s with 22.4psi vacuum 2.7m deep (18.7psia)
@11:19:32.70 Cartridge 6 filtering 232/1000ml at 0.31ml/s with 25.2psi vacuum 5.0m deep (22.0psia)
@11:20:32.71 Cartridge 6 filtering 249/1000ml at 0.30ml/s with 21.2psi vacuum 3.3m deep (19.6psia)
@11:21:32.70 Cartridge 6 filtering 265/1000ml at 0.31ml/s with 25.1psi vacuum 3.9m deep (20.5psia)
@11:22:32.71 Cartridge 6 filtering 281/1000ml at 0.31ml/s with 24.1psi vacuum 4.3m deep (21.0psia)
@11:23:32.70 Cartridge 6 filtering 298/1000ml at 0.22ml/s with 24.8psi vacuum 2.0m deep (17.6psia)
@11:24:32.77 Cartridge 6 filtering 314/1000ml at 0.23ml/s with 20.8psi vacuum 3.8m deep (20.3psia)
@11:25:32.70 Cartridge 6 filtering 330/1000ml at 0.30ml/s with 25.7psi vacuum 2.2m deep (18.0psia)
@11:26:32.71 Cartridge 6 filtering 346/1000ml at 0.22ml/s with 23.3psi vacuum 4.6m deep (21.5psia)
@11:27:32.70 Cartridge 6 filtering 361/1000ml at 0.23ml/s with 28.5psi vacuum
@11:28:32.71 Cartridge 6 filtering 377/1000ml at 0.28ml/s with 23.0psi vacuum 5.0m deep (22.0psia)
@11:29:32.70 Cartridge 6 filtering 393/1000ml at 0.27ml/s with 27.1psi vacuum 4.1m deep (20.7psia)
@11:30:32.71 Cartridge 6 filtering 408/1000ml at 0.29ml/s with 25.1psi vacuum 2.2m deep (18.0psia)
@11:31:32.70 Cartridge 6 filtering 424/1000ml at 0.27ml/s with 21.4psi vacuum 5.9m deep (23.4psia)
@11:32:32.71 Cartridge 6 filtering 439/1000ml at 0.28ml/s with 26.2psi vacuum 2.1m deep (17.7psia)
@11:33:32.70 Cartridge 6 filtering 454/1000ml at 0.28ml/s with 23.6psi vacuum 4.5m deep (21.3psia)
@11:34:32.71 Cartridge 6 filtering 469/1000ml at 0.27ml/s with 22.7psi vacuum 4.2m deep (20.9psia)
@11:35:32.70 Cartridge 6 filtering 484/1000ml at 0.26ml/s with 26.7psi vacuum 1.8m deep (17.3psia)
@11:36:32.71 Cartridge 6 filtering 499/1000ml at 0.21ml/s with 27.9psi vacuum 3.3m deep (19.6psia)
@11:37:32.70 Cartridge 6 filtering 514/1000ml at 0.26ml/s with 26.5psi vacuum
@11:38:32.71 Cartridge 6 filtering 529/1000ml at 0.25ml/s with 20.9psi vacuum 6.2m deep (23.8psia)
@11:39:32.70 Cartridge 6 filtering 543/1000ml at 0.27ml/s with 23.7psi vacuum
@11:40:32.71 Cartridge 6 filtering 558/1000ml at 0.26ml/s with 23.5psi vacuum 5.3m deep (22.6psia)
@11:41:32.70 Cartridge 6 filtering 572/1000ml at 0.20ml/s with 24.5psi vacuum 2.2m deep (17.9psia)
@11:42:32.71 Cartridge 6 filtering 587/1000ml at 0.24ml/s with 22.0psi vacuum 3.9m deep (20.5psia)
@11:43:32.70 Cartridge 6 filtering 601/1000ml at 0.24ml/s with 22.6psi vacuum 3.5m deep (19.8psia)
@11:44:32.71 Cartridge 6 filtering 615/1000ml at 0.20ml/s with 24.7psi vacuum 2.6m deep (18.6psia)
@11:45:32.70 Cartridge 6 filtering 629/1000ml at 0.25ml/s with 24.2psi vacuum 4.6m deep (21.4psia)
@11:46:32.71 Cartridge 6 filtering 643/1000ml at 0.26ml/s with 26.4psi vacuum 2.2m deep (18.0psia)
@11:47:32.70 Cartridge 6 filtering 657/1000ml at 0.26ml/s with 24.6psi vacuum 4.9m deep (22.0psia)
@11:48:32.71 Cartridge 6 filtering 671/1000ml at 0.24ml/s with 24.5psi vacuum
@11:49:32.70 Cartridge 6 filtering 685/1000ml at 0.20ml/s with 27.4psi vacuum 2.6m deep (18.5psia)
@11:50:32.71 Cartridge 6 filtering 699/1000ml at 0.23ml/s with 23.3psi vacuum 3.3m deep (19.6psia)
@11:51:32.70 Cartridge 6 filtering 713/1000ml at 0.23ml/s with 23.4psi vacuum 2.9m deep (19.0psia)
@11:52:32.71 Cartridge 6 filtering 726/1000ml at 0.23ml/s with 21.9psi vacuum 5.7m deep (23.1psia)
@11:53:32.70 Cartridge 6 filtering 740/1000ml at 0.21ml/s with 27.3psi vacuum 1.9m deep (17.5psia)
@11:54:32.71 Cartridge 6 filtering 753/1000ml at 0.22ml/s with 21.4psi vacuum 5.8m deep (23.3psia)
@11:55:32.70 Cartridge 6 filtering 767/1000ml at 0.22ml/s with 22.5psi vacuum 2.1m deep (17.9psia)
@11:56:32.71 Cartridge 6 filtering 780/1000ml at 0.21ml/s with 23.3psi vacuum 3.6m deep (20.0psia)
@11:57:32.70 Cartridge 6 filtering 793/1000ml at 0.22ml/s with 26.5psi vacuum 3.3m deep (19.6psia)
@11:58:32.71 Cartridge 6 filtering 806/1000ml at 0.21ml/s with 22.6psi vacuum 3.4m deep (19.8psia)
@11:59:32.70 Cartridge 6 filtering 820/1000ml at 0.22ml/s with 23.5psi vacuum 4.6m deep (21.5psia)
@12:00:32.71 Cartridge 6 filtering 833/1000ml at 0.22ml/s with 23.9psi vacuum 1.8m deep (17.3psia)
@12:01:32.70 Cartridge 6 filtering 846/1000ml at 0.20ml/s with 25.9psi vacuum 4.5m deep (21.3psia)
@12:02:32.71 Cartridge 6 filtering 859/1000ml at 0.24ml/s with 23.8psi vacuum 2.1m deep (17.9psia)
@12:03:32.70 Cartridge 6 filtering 871/1000ml at 0.20ml/s with 26.1psi vacuum 4.7m deep (21.7psia)
@12:04:32.71 Cartridge 6 filtering 884/1000ml at 0.20ml/s with 25.5psi vacuum 1.7m deep (17.2psia)
@12:05:32.70 Cartridge 6 filtering 897/1000ml at 0.20ml/s with 26.4psi vacuum 4.3m deep (21.1psia)
@12:06:32.71 Cartridge 6 filtering 910/1000ml at 0.21ml/s with 27.0psi vacuum
@12:07:32.70 Cartridge 6 filtering 922/1000ml at 0.20ml/s with 24.5psi vacuum 3.8m deep (20.3psia)
@12:08:32.71 Cartridge 6 filtering 935/1000ml at 0.23ml/s with 24.5psi vacuum 3.4m deep (19.8psia)
@12:09:32.70 Cartridge 6 filtering 947/1000ml at 0.20ml/s with 26.5psi vacuum 3.4m deep (19.7psia)
@12:10:32.71 Cartridge 6 filtering 960/1000ml at 0.20ml/s with 28.3psi vacuum 4.1m deep (20.7psia)
@12:11:32.70 Cartridge 6 filtering 972/1000ml at 0.22ml/s with 25.0psi vacuum 2.1m deep (17.9psia)
@12:12:32.71 Cartridge 6 filtering 985/1000ml at 0.20ml/s with 24.8psi vacuum 5.1m deep (22.2psia)
@12:13:32.70 Cartridge 6 filtering 997/1000ml at 0.20ml/s with 29.5psi vacuum
@12:13:47.41 <FILTERING> Pressure.coast
@12:13:47.53 <closeIntake> Intake.close
@12:13:47.58 <closeExhaust> Exhaust.close
@12:13:47.73 <FILTERING> Toroid.in :hold
@12:13:47.79 Toroid.seek :bypass
@12:13:49.40 Toroid.in :default
@12:13:49.46 Toroid.seek :clear
@12:13:51.07 Sampled  1000.0ml
@12:13:52.48 <LRAUV> -> Cmd.startProcessing
@12:13:54.33 <PROCESSING> SPR.command \"read_sequences\"
@12:13:54.38 SPR.command \"stats_on\"
@12:13:54.42 SPR.command \"start 0 1\"
@12:13:56.43 <SPRlogger> Begun log /var/log/esp/spr21apr2512h.spr
@12:13:57.45 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3)
@12:13:58.99 <PROCESSING> Handoff.select :waste
@12:14:05.67 Syringe tare is 20% of full scale
@12:14:05.69 PinPort.in :locking
@12:14:05.76 PinPort.seek :locked
@12:14:06.24 <posArchive> Archive.in :fast
@12:14:06.30 <posLyS2> Lysis2.in :fast
@12:14:06.33 <PROCESSING> Lysis1.seek 1350uL
@12:14:06.46 <posLyS2> Lysis2.seek :full
@12:14:06.51 <posArchive> Archive.seek :full
@12:14:08.55 <PROCESSING> Plunge.seek :reagent
@12:14:09.45 Evacuating puck with filter bubble point of 50psi
@12:14:09.48 PinPort.seek :locked
@12:14:09.56 Handoff.select :waste
@12:14:16.14 Air syringe pumping up to 1 stroke to reach 8psi
@12:14:16.15 Air stroke #1 of 1
@12:14:16.23 Air.in :pressurize
@12:14:16.29 Air.seek :pressurized
@12:14:27.56 0.1psi after pressurize
@12:14:27.57 Air.in :compress
@12:14:27.64 Air.seek :evac
@12:14:31.73 0.6psi after compress
@12:14:44.30 0.3psi after compress (stable)
@12:14:44.31 Air.in :default
@12:14:44.38 Air.hold
@12:14:44.56 at 0.2psi Opened to waste
@12:14:44.57 Handoff.select :blocked
@12:14:44.70 Archive syringe pumping up to 5 strokes to reach 50psi
@12:14:44.71 Archive stroke #1 of 5
@12:14:44.79 Archive.in :pressurize
@12:14:44.86 Archive.seek :pressurized
@12:14:57.61 7.7psi after pressurize
@12:14:57.62 Archive.in :compress
@12:14:57.69 Archive.seek :empty
@12:15:13.60 32.7psi after compress
@12:15:30.12 Reached 50.0 of 50.0psi after pumping 1730uL (1.00 strokes)
@12:15:30.13 Archive.in :default
@12:15:30.19 Archive.hold
@12:15:30.27 Handoff.select :waste
@12:15:38.50 delay 1:00
@12:16:38.52 Handoff.select :blocked
@12:17:01.19 Handoff.select :waste
@12:17:01.31 0.1psi at evacEnd
@12:17:01.32 Handoff.select :blocked
@12:17:01.45 Archive.in :fast
@12:17:01.52 Archive.seek :topVent
@12:17:15.26 Stable @0.3psi after Handoff blocked
@12:17:15.29 Archive syringe pumping up to 5 strokes to reach 30psi
@12:17:15.31 Archive stroke #1 of 5
@12:17:15.40 Archive.in :pressurize
@12:17:15.46 Archive.seek :pressurized
@12:17:28.20 0.3psi after pressurize
@12:17:28.21 Archive.in :compress
@12:17:28.29 Archive.seek :empty
@12:17:44.18 0.5psi after compress
@12:17:56.75 0.7psi after compress (stable)
@12:17:56.77 Archive.in :gulp
@12:17:56.85 Archive.seek :topVent
@12:18:04.06 1.5psi after gulp
@12:18:16.64 2.3psi after gulp (stable)
@12:18:16.66 Archive stroke #2 of 5
@12:18:16.76 Archive.in :pressurize
@12:18:16.83 Archive.seek :pressurized
@12:18:29.57 3.5psi after pressurize
@12:18:29.58 Archive.in :compress
@12:18:29.66 Archive.seek :empty
@12:18:45.55 5.4psi after compress
@12:19:18.71 Reached 30.3 of 30.3psi after pumping 3460uL (2.00 strokes)
@12:19:18.73 Archive.in :default
@12:19:18.80 Archive.hold
@12:19:18.86 Handoff.select :waste
@12:19:36.04 delay 1:00
@12:20:36.07 Handoff.select :blocked
@12:21:03.75 Handoff.select :waste
@12:21:03.86 3.9psi at evacEnd
@12:21:03.87 Air.seek :fullVent
@12:21:05.32 Archive.in :fast
@12:21:05.38 Archive.seek :topVent
@12:21:12.52 Seawater evacuated, Presure above/below filter released. Lysis begins
@12:21:12.61 Lysis1.in :slow
@12:21:12.69 Lysis1.seek 1050uL
@12:21:42.73 delay 5 seconds
@12:21:47.75 Handoff.select :blocked
@12:21:47.91 Lysis1.seek 450uL
@12:22:47.90 delay 3 seconds
@12:22:51.00 Air.in to mix into 1.37806 parts Lysis1
@12:22:51.58 <Airmixer> Air.seek :evac
@12:22:51.65 <PROCESSING> Lysis1.seek 300uL
@12:23:06.73 Lysis1.seek :empty
@12:23:36.84 Lysis buffer delivered @15.4psi
@12:23:36.85 Polling Can every 2:00
@12:23:37.40 PuckHeater.configure :default #ambient 11.6C
@12:23:37.44 PuckHeater.seek 80.2C
@12:24:49.01 PuckHeater.hold 2:00,80.2C
@12:26:49.14 Polling Can every 2:00
@12:26:49.68 PuckHeater.configure :default #ambient 11.7C
@12:26:49.72 PuckHeater.hold 30 seconds,79.2C
@12:27:19.90 Polling Can every 2:00
@12:27:20.46 PuckHeater.configure :default #ambient 11.7C
@12:27:20.50 PuckHeater.hold 30 seconds,78.6C
@12:27:50.71 Polling Can every 2:00
@12:27:51.26 PuckHeater.configure :default #ambient 11.6C
@12:27:51.31 PuckHeater.hold 10:00,78.1C
@12:37:51.40 Lysis complete. Syringe pressure @20.8psi
@12:37:51.42 Handoff.select :blocked
@12:37:51.48 Archive syringe pumping up to 3 strokes to reach 40psi
@12:37:51.49 Archive stroke #1 of 3
@12:37:51.57 Archive.in :pressurize
@12:37:51.64 Archive.seek :pressurized
@12:38:04.37 20.8psi after pressurize
@12:38:04.38 Archive.in :compress
@12:38:04.46 Archive.seek :empty
@12:38:15.51 Reached 40.2 of 40.0psi after pumping 1627uL (0.94 strokes)
@12:38:15.52 Archive.in :default
@12:38:15.59 Archive.hold
@12:38:15.66 delay 2 seconds
@12:38:17.68 Archive.in :fast
@12:38:17.74 Archive.seek :full
@12:38:24.61 Lysis1.seek 138uL
@12:38:38.49 delay 1 second
@12:38:39.52 <fillArchive> Archive.seek :full
@12:38:39.54 <PROCESSING> Lysis1.seek :empty
@12:38:53.54 Initial air pressure @56.3psi
@12:38:53.61 Lysis1.in :extract
@12:38:53.69 Lysis1.seek 600uL,nil
@12:38:55.95 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@12:38:55.96 Archive stroke #1 of 6
@12:38:56.05 Archive.in :pressurize
@12:38:56.11 Archive.seek :pressurized
@12:39:08.84 16.0psi after pressurize
@12:39:08.85 Archive.in :compress
@12:39:08.92 Archive.seek :empty
@12:39:24.86 15.7psi after compress
@12:39:32.98 Archive.in :default
@12:39:33.03 Archive.hold
@12:39:33.15 <PROCESSING> Initial 600uL lysate @16.2psi
@12:39:33.16 Handoff.select :blocked
@12:39:33.26 Initial air pressure @16.4psi
@12:39:33.32 Lysis1.seek 825uL,nil
@12:39:33.96 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@12:39:33.97 Archive stroke #1 of 6
@12:39:34.06 Archive.in :compress
@12:39:34.12 Archive.seek :empty
@12:39:34.29 15.0psi after compress
@12:39:46.89 15.9psi after compress (stable)
@12:39:52.06 Recovering 3.5uL/s
@12:39:57.12 Recovering 3.6uL/s
@12:40:02.18 Recovering 2.7uL/s
@12:40:07.24 Recovering 2.7uL/s
@12:40:12.30 Recovering 2.1uL/s
@12:40:17.37 Recovering 2.3uL/s
@12:40:22.43 Recovering 2.1uL/s
@12:40:27.51 Archive.in :gulp
@12:40:27.58 Archive.seek :topVent
@12:40:34.79 16.1psi after gulp
@12:40:47.37 15.8psi after gulp (stable)
@12:40:47.38 Archive stroke #2 of 6
@12:40:47.52 Archive.in :pressurize
@12:40:47.58 Archive.seek :pressurized
@12:40:54.72 Archive.in :default
@12:40:54.77 Archive.hold
@12:40:55.36 <PROCESSING> Recovered 825uL lysate @15.6psi
@12:40:55.38 Air.in :halfSpeed
@12:40:55.45 Air.seek :fullVent
@12:40:57.69 Puck Top Vented, bottom @2.4psi
@12:40:57.70 Handoff.select :waste
@12:40:57.83 delay 5 seconds
@12:41:02.89 Handoff open to waste, bottom @0.0psi
@12:41:02.90 Air.in :default
@12:41:02.97 Air.seek :evac
@12:41:04.42 Lysis2.in :DAmix
@12:41:04.54 Lysis1.in :DAmix
@12:41:05.04 <Lysis2mixer> Lysis2.seek 1480uL
@12:41:05.08 <PROCESSING> Lysis1.seek 575uL
@12:41:40.17 delay 5 seconds
@12:41:45.19 Handoff.select SPR
@12:41:45.30 SPR.command \"start 2 14\"
@12:41:46.59 delay 10 seconds
@12:41:56.61 SPR.command \"start 1 0\"
@12:41:59.85 Waiting up to 4:00 for SPR to enter state 1
@12:44:02.21 SPR.command \"hold_on\"
@12:44:04.12 SPR Dilution 1 of 1 -- Running Experiment 1
@12:44:04.54 <Lysis2mixer> Lysis2.seek 1180uL
@12:44:04.57 <PROCESSING> Lysis1.seek 275uL
@12:44:46.61 volL1 = 300.0 and volL2 = 300.0
@12:44:46.62 delay 15 seconds
@12:45:01.65 SPR.command \"hold_off\"
@12:45:04.30 Waiting up to 38:00 for SPR to enter state 7
@13:11:46.88 SPR Analysis has Completed
@13:11:46.90 Handoff.select :waste
@13:11:47.82 <SPRlogger> Ended log /var/log/esp/spr21apr2512h.spr
@13:11:47.83 <PROCESSING> SPR.command \"stats_off\"
@13:11:49.45 <LRAUV> -> Cmd.stop
@13:11:49.46 Archive.stop
@13:11:49.58 <closeHandoff> Handoff.select :close
@13:11:49.72 <LRAUV> Gate.power :heat,:OFF
@13:11:49.74 Gate.power :cartridge,:OFF
@13:11:49.83 Carousel.seek 6
@13:11:50.00 Plunge.seek :locked
@13:11:50.88 PinPort.in :unlocking
@13:11:50.94 PinPort.seek :clear
@13:11:51.48 <Archive> Archive.in :unlocking
@13:11:51.58 Archive.seek :clear
@13:11:51.66 <Air> Air.in :unlocking
@13:11:51.78 Air.seek :clear
@13:11:51.86 <Lysis1> Lysis1.in :unlocking
@13:11:52.00 Lysis1.seek :clear
@13:11:52.03 <Lysis2> Lysis2.in :unlocking
@13:11:52.13 Lysis2.seek :clear
@13:11:55.53 Slide::Error in LRAUV -- Lysis Syringe #1 (LyS1) positionErr at 1056uL (sensed 1031uL)
@13:11:55.55 <LRAUV> Archive.coast
@13:11:55.60 Air.coast
@13:11:55.66 Lysis1.coast
@13:11:55.70 Lysis2.coast
@13:11:55.75 Plunge.coast
@13:11:55.80 SamplePump.coast
@13:11:55.85 Toroid.coast
@13:11:55.91 Carousel.coast
@13:11:55.96 PinPort.coast
@13:11:56.15 Gate.power :motor,:OFF
@13:11:56.17 Gate.power :core,:OFF
@13:11:56.19 Gate.power :analytic1,:OFF
@13:11:56.21 Gate.power :analytic2,:OFF
@13:11:56.23 Gate.power :analytic3,:OFF
@13:11:56.25 Gate.power :main,:OFF
@13:11:56.27 ready to power off
##-- file closed 2021-04-25T22:19:35.370Z

##-- file opened 2021-04-25T22:19:35.371Z
@15:19:35.36 -> showStatus
@15:19:37.38 -> Cmd.loadCartridge -6
@15:19:37.52 Gate.power :main,:ON,limit to 5000mA
@15:19:37.53 Gate.power :analytic1,:ON,limit to 5000mA
@15:19:37.55 Gate.power :analytic2,:ON,limit to 5000mA
@15:19:37.58 Gate.power :analytic3,:ON,limit to 5000mA
@15:19:37.70 Powering core bus
@15:19:40.01 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@15:19:40.13 <LRAUV> Archive.configure :default
@15:19:40.26 Air.configure :default
@15:19:40.36 Lysis1.configure :default
@15:19:40.46 Lysis2.configure :default
@15:19:40.58 Plunge.configure :default
@15:19:40.71 SamplePump.configure :default
@15:19:40.86 ExtraPump.configure :default
@15:19:40.98 Toroid.configure :default
@15:19:41.10 Carousel.configure :default
@15:19:41.23 Pressure.configure :default
@15:19:41.35 PinPort.configure :default
@15:19:41.41 All elf controllers running firmware version 4.741
@15:19:41.44 Gate.power :motor,:ON,limit to 5000mA
@15:19:41.60 <Archive_home> Archive.in :default
@15:19:41.90 Archive.home.jog 0
@15:19:42.05 <Toroid_home> Toroid.in :default
@15:19:42.13 <Plunge_home> Plunge.in :default
@15:19:42.16 <Lysis1_home> Lysis1.in :default
@15:19:42.21 <PinPort_home> PinPort.in :default
@15:19:42.27 <Lysis2_home> Lysis2.in :default
@15:19:42.30 <Air_home> Air.in :default
@15:19:42.47 <Toroid_home> Toroid.home.jog 0
@15:19:42.52 <Plunge_home> Plunge.home.jog 0
@15:19:42.54 <Lysis1_home> Lysis1.home.jog 0
@15:19:42.57 <PinPort_home> PinPort.home.jog 0
@15:19:42.59 <Lysis2_home> Lysis2.home.jog 0
@15:19:42.63 <Air_home> Air.home.jog 0
@15:19:43.05 <Archive_home> Archive.in :homing
@15:19:43.31 <Toroid_home> Toroid.in :homing
@15:19:43.35 <Plunge_home> Plunge.in :homing
@15:19:43.38 <Lysis1_home> Lysis1.in :homing
@15:19:43.42 <Archive_home> Archive.jog 151
@15:19:43.46 <PinPort_home> PinPort.in :homing
@15:19:43.48 <Lysis2_home> Lysis2.in :homing
@15:19:43.52 <Air_home> Air.in :homing
@15:19:43.74 <Toroid_home> Toroid.jog 620
@15:19:43.77 <Lysis1_home> Lysis1.jog 4113
@15:19:43.80 <Plunge_home> Plunge.jog 1579
@15:19:43.83 <PinPort_home> PinPort.jog -108
@15:19:43.86 <Lysis2_home> Lysis2.jog 1429
@15:19:43.90 <Air_home> Air.jog 173
@15:19:44.13 <Archive_home> Archive.setRawPosition! 50
@15:19:44.18 Archive.seek :home
@15:19:44.26 <PinPort_home> PinPort.setRawPosition! 0
@15:19:44.30 PinPort.seek :home
@15:19:44.39 PinPort.in :default
@15:19:44.49 <Air_home> Air.setRawPosition! 50
@15:19:44.50 <Archive_home> Archive.in :default
@15:19:44.57 <Air_home> Air.seek :home
@15:19:44.88 Air.in :default
@15:19:45.19 <Plunge_home> Plunge.setRawPosition! 50
@15:19:45.21 <Lysis2_home> Lysis2.setRawPosition! 50
@15:19:45.29 <Plunge_home> Plunge.seek :home
@15:19:45.32 <Lysis2_home> Lysis2.seek :home
@15:19:45.61 <Plunge_home> Plunge.in :default
@15:19:45.69 <Lysis2_home> Lysis2.in :default
@15:19:45.97 <Toroid_home> Toroid.setRawPosition! 0
@15:19:46.01 Toroid.seek :home
@15:19:46.11 Toroid.in :default
@15:19:46.20 <Lysis1_home> Lysis1.setRawPosition! 50
@15:19:46.24 Lysis1.seek :home
@15:19:46.52 Lysis1.in :default
@15:19:46.89 <LRAUV> Toroid.seek :clear
@15:19:48.50 Carousel.in :default
@15:19:48.56 Carousel.home.jog 0
@15:19:49.05 Carousel.in :default
@15:19:49.11 Carousel.seek 6
@15:19:49.25 Selecting Cartridge 8
@15:19:49.49 Carousel.seek 8
@15:19:50.17 Lysis1.in :fast
@15:19:50.24 Lysis1.seek :locked
@15:19:52.83 <Plunge> Plunge.seek :locked
@15:19:52.95 <Air> Air.in :fast
@15:19:53.02 Air.seek :locked
@15:19:55.68 <LRAUV> Powering cartridge bus
@15:19:57.80 Gate.setIR 1750,5000,0,6
@15:19:57.93 Gate.setIR 0,5000,0,6
@15:19:57.98 Gate.power :cartridge,:OFF
@15:19:58.51 Mounted archive Cartridge
@15:20:16.98 -> Cmd.startFiltering
@15:20:17.43 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@15:20:17.44 SamplePump.setPosition! 0ml
@15:20:17.48 Bypass.open
@15:20:18.97 Intake.open
@15:20:19.75 Sea 4.0m deep (20.6psia)
@15:20:19.77 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@15:20:22.35 Vacuum tare is 51% of full scale
@15:20:22.48 Pressure.in :default
@15:20:22.54 Pressure.hold
@15:20:22.71 SamplePump.in :prime
@15:20:22.77 SamplePump.seek 10ml,1:21
@15:20:32.17 Pressure.coast
@15:20:32.22 SamplePump.setPosition! 0ml
@15:20:32.27 Exhaust.open
@15:20:33.71 <closeBypass> Bypass.close
@15:20:35.24 <FILTERING> Pressure.in :default
@15:20:35.30 Pressure.hold
@15:20:35.41 Sea 4.1m deep (20.7psia)
@15:20:35.54 SamplePump.in :prime
@15:20:35.62 SamplePump.seek 100ml,8:51
@15:22:45.43 Pressure.coast
@15:22:45.49 Bypass.open
@15:22:46.17 Intake.close
@15:22:47.01 Toroid.in :hold
@15:22:47.07 Toroid.seek :crack
@15:22:48.63 Toroid.seek :cartridge
@15:22:50.11 Toroid.in :default
@15:22:50.18 Toroid.seek :clear
@15:22:52.14 Pressurizing cartridge to 26psi above 21.8psia
@15:22:52.15 SamplePump.setPosition! 0ml
@15:22:52.20 Intake.open
@15:22:52.93 Bypass.close
@15:22:53.65 Exhaust.close
@15:22:54.48 Pressure.in :default
@15:22:54.54 Pressure.hold
@15:22:54.72 SamplePump.in :charge
@15:22:54.78 SamplePump.seek 2.3ml,42 seconds
@15:23:03.13 Pressure.coast
@15:23:03.18 Intake.close
@15:23:03.99 Pressurized cartridge with 0.308ml to 26.9psi
@15:23:04.00 Isolated @45.4psia (23.6psi)
@15:23:04.01 Verifying cartridge holds at least 0.75..0.85 of 45.4psia
@15:23:04.04 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi
@15:23:08.66 Sampling 1000.0ml
@15:23:08.68 SamplePump.setPosition! 0ml
@15:23:08.73 Exhaust.open
@15:23:09.45 Intake.open
@15:23:10.28 Pressure.in :default
@15:23:10.35 Pressure.hold
@15:23:10.46 Sea 6.5m deep (24.4psia)
@15:23:10.60 SamplePump.in :sample
@15:23:10.67 SamplePump.seek 1000ml,1:23:53
@15:23:13.32 <sampling> Cartridge 8 filtering 1/1000ml at 0.35ml/s with 22.4psi vacuum 4.6m deep (21.5psia)
@15:24:13.31 Cartridge 8 filtering 21/1000ml at 0.33ml/s with 20.7psi vacuum 3.5m deep (19.9psia)
@15:25:13.32 Cartridge 8 filtering 41/1000ml at 0.34ml/s with 22.6psi vacuum 5.0m deep (22.1psia)
@15:26:13.31 Cartridge 8 filtering 60/1000ml at 0.30ml/s with 29.7psi vacuum
@15:27:13.32 Cartridge 8 filtering 80/1000ml at 0.36ml/s with 22.1psi vacuum 5.2m deep (22.3psia)
@15:28:13.31 Cartridge 8 filtering 99/1000ml at 0.34ml/s with 26.1psi vacuum 4.7m deep (21.7psia)
@15:29:13.32 Cartridge 8 filtering 118/1000ml at 0.33ml/s with 23.7psi vacuum 3.8m deep (20.3psia)
@15:30:13.31 Cartridge 8 filtering 137/1000ml at 0.33ml/s with 25.2psi vacuum 3.3m deep (19.5psia)
@15:31:13.32 Cartridge 8 filtering 155/1000ml at 0.26ml/s with 26.5psi vacuum
@15:32:13.31 Cartridge 8 filtering 174/1000ml at 0.26ml/s with 22.4psi vacuum 3.3m deep (19.6psia)
@15:33:13.32 Cartridge 8 filtering 192/1000ml at 0.34ml/s with 23.5psi vacuum 3.5m deep (19.9psia)
@15:34:13.31 Cartridge 8 filtering 211/1000ml at 0.31ml/s with 26.6psi vacuum 3.9m deep (20.4psia)
@15:35:13.32 Cartridge 8 filtering 229/1000ml at 0.33ml/s with 19.1psi vacuum 4.6m deep (21.5psia)
@15:36:13.31 Cartridge 8 filtering 247/1000ml at 0.31ml/s with 28.3psi vacuum 2.3m deep (18.1psia)
@15:37:13.32 Cartridge 8 filtering 265/1000ml at 0.34ml/s with 24.3psi vacuum 3.0m deep (19.2psia)
@15:38:13.31 Cartridge 8 filtering 282/1000ml at 0.24ml/s with 27.4psi vacuum
@15:39:13.32 Cartridge 8 filtering 300/1000ml at 0.27ml/s with 29.9psi vacuum 2.5m deep (18.3psia)
@15:40:13.31 Cartridge 8 filtering 318/1000ml at 0.31ml/s with 25.8psi vacuum 2.7m deep (18.6psia)
@15:41:13.32 Cartridge 8 filtering 335/1000ml at 0.31ml/s with 17.6psi vacuum 6.9m deep (24.9psia)
@15:42:13.31 Cartridge 8 filtering 352/1000ml at 0.31ml/s with 23.5psi vacuum 3.3m deep (19.5psia)
@15:43:13.32 Cartridge 8 filtering 369/1000ml at 0.33ml/s with 24.1psi vacuum 5.6m deep (23.0psia)
@15:44:13.31 Cartridge 8 filtering 386/1000ml at 0.31ml/s with 19.5psi vacuum 5.0m deep (22.1psia)
@15:45:13.32 Cartridge 8 filtering 403/1000ml at 0.23ml/s with 21.7psi vacuum 4.4m deep (21.2psia)
@15:46:13.31 Cartridge 8 filtering 420/1000ml at 0.25ml/s with 21.3psi vacuum 2.6m deep (18.6psia)
@15:47:13.32 Cartridge 8 filtering 436/1000ml at 0.28ml/s with 28.2psi vacuum 1.6m deep (17.1psia)
@15:48:13.31 Cartridge 8 filtering 453/1000ml at 0.24ml/s with 21.8psi vacuum 3.2m deep (19.4psia)
@15:49:13.32 Cartridge 8 filtering 469/1000ml at 0.23ml/s with 23.5psi vacuum 2.7m deep (18.7psia)
@15:50:13.31 Cartridge 8 filtering 486/1000ml at 0.27ml/s with 22.5psi vacuum
@15:51:13.32 Cartridge 8 filtering 502/1000ml at 0.31ml/s with 26.1psi vacuum 4.5m deep (21.3psia)
@15:52:13.31 Cartridge 8 filtering 518/1000ml at 0.27ml/s with 18.7psi vacuum 3.5m deep (19.9psia)
@15:53:13.32 Cartridge 8 filtering 534/1000ml at 0.21ml/s with 24.4psi vacuum
@15:54:13.31 Cartridge 8 filtering 550/1000ml at 0.23ml/s with 29.4psi vacuum 3.0m deep (19.1psia)
@15:55:13.32 Cartridge 8 filtering 565/1000ml at 0.26ml/s with 20.8psi vacuum
@15:56:13.31 Cartridge 8 filtering 581/1000ml at 0.29ml/s with 22.2psi vacuum 6.3m deep (23.9psia)
@15:57:13.32 Cartridge 8 filtering 596/1000ml at 0.27ml/s with 23.7psi vacuum 3.4m deep (19.7psia)
@15:58:13.31 Cartridge 8 filtering 611/1000ml at 0.29ml/s with 18.9psi vacuum 7.9m deep (26.4psia)
@15:59:13.32 Cartridge 8 filtering 627/1000ml at 0.25ml/s with 24.8psi vacuum
@16:00:13.31 Cartridge 8 filtering 642/1000ml at 0.25ml/s with 23.6psi vacuum 5.4m deep (22.7psia)
@16:01:13.32 Cartridge 8 filtering 657/1000ml at 0.23ml/s with 28.1psi vacuum 1.9m deep (17.4psia)
@16:02:13.31 Cartridge 8 filtering 671/1000ml at 0.25ml/s with 25.5psi vacuum 4.5m deep (21.3psia)
@16:03:13.32 Cartridge 8 filtering 686/1000ml at 0.25ml/s with 27.7psi vacuum 1.6m deep (17.1psia)
@16:04:13.31 Cartridge 8 filtering 701/1000ml at 0.28ml/s with 25.1psi vacuum 3.7m deep (20.2psia)
@16:05:13.32 Cartridge 8 filtering 715/1000ml at 0.22ml/s with 22.5psi vacuum 4.8m deep (21.7psia)
@16:06:13.32 Cartridge 8 filtering 729/1000ml at 0.25ml/s with 20.7psi vacuum 6.0m deep (23.6psia)
@16:07:13.32 Cartridge 8 filtering 744/1000ml at 0.20ml/s with 23.3psi vacuum 3.0m deep (19.1psia)
@16:08:13.31 Cartridge 8 filtering 758/1000ml at 0.24ml/s with 25.9psi vacuum
@16:09:13.32 Cartridge 8 filtering 772/1000ml at 0.23ml/s with 23.6psi vacuum 1.7m deep (17.2psia)
@16:10:13.31 Cartridge 8 filtering 786/1000ml at 0.24ml/s with 23.3psi vacuum 2.9m deep (19.0psia)
@16:11:13.32 Cartridge 8 filtering 799/1000ml at 0.22ml/s with 24.6psi vacuum 3.8m deep (20.2psia)
@16:12:13.31 Cartridge 8 filtering 813/1000ml at 0.21ml/s with 22.7psi vacuum 3.0m deep (19.1psia)
@16:13:13.32 Cartridge 8 filtering 827/1000ml at 0.21ml/s with 22.5psi vacuum 5.0m deep (22.0psia)
@16:14:13.31 Cartridge 8 filtering 840/1000ml at 0.24ml/s with 26.0psi vacuum 3.2m deep (19.5psia)
@16:15:13.32 Cartridge 8 filtering 854/1000ml at 0.21ml/s with 22.1psi vacuum 3.3m deep (19.6psia)
@16:16:13.31 Cartridge 8 filtering 867/1000ml at 0.23ml/s with 24.8psi vacuum 1.9m deep (17.5psia)
@16:17:13.32 Cartridge 8 filtering 881/1000ml at 0.24ml/s with 21.3psi vacuum 6.7m deep (24.5psia)
@16:18:13.32 Cartridge 8 filtering 894/1000ml at 0.20ml/s with 28.5psi vacuum 3.4m deep (19.8psia)
@16:19:13.32 Cartridge 8 filtering 907/1000ml at 0.22ml/s with 23.1psi vacuum 2.7m deep (18.7psia)
@16:20:13.32 Cartridge 8 filtering 920/1000ml at 0.22ml/s with 25.9psi vacuum 4.6m deep (21.5psia)
@16:21:13.32 Cartridge 8 filtering 933/1000ml at 0.22ml/s with 23.6psi vacuum 2.5m deep (18.4psia)
@16:22:13.32 Cartridge 8 filtering 946/1000ml at 0.20ml/s with 23.8psi vacuum 5.4m deep (22.7psia)
@16:23:13.32 Cartridge 8 filtering 959/1000ml at 0.21ml/s with 18.9psi vacuum
@16:24:13.32 Cartridge 8 filtering 972/1000ml at 0.22ml/s with 26.2psi vacuum 5.3m deep (22.6psia)
@16:25:13.32 Cartridge 8 filtering 985/1000ml at 0.20ml/s with 22.8psi vacuum 1.6m deep (17.0psia)
@16:26:13.32 Cartridge 8 filtering 998/1000ml at 0.23ml/s with 26.3psi vacuum 3.9m deep (20.5psia)
@16:26:24.98 <FILTERING> Pressure.coast
@16:26:25.10 <closeIntake> Intake.close
@16:26:25.15 <closeExhaust> Exhaust.close
@16:26:25.29 <FILTERING> Toroid.in :hold
@16:26:25.35 Toroid.seek :bypass
@16:26:26.97 Toroid.in :default
@16:26:27.03 Toroid.seek :clear
@16:26:28.64 Sampled  1000.0ml
@16:26:30.20 <LRAUV> -> Cmd.startProcessing
@16:26:30.57 <PROCESSING> Plunge.seek :reagent
@16:26:31.41 Sample loop closed. Ready to Deliver Reagents
@16:26:31.42 Archive.seek :sealed
@16:26:35.68 Archive.seek :evac
@16:26:49.61 delay 1:00
@16:27:49.64 Archive.seek :clear
@16:28:07.36 RNAlater soak for 10:00
@16:38:07.81 Archive.seek :tVent
@16:38:10.38 delay 1 second
@16:38:11.40 Archive.seek :evac
@16:38:27.02 delay 10 seconds
@16:38:37.05 Sample preserved and evacuated
@16:38:38.24 <LRAUV> -> Cmd.stop
@16:38:38.38 Carousel.seek 8
@16:38:38.55 Plunge.seek :locked
@16:38:39.50 <Archive> Archive.in :unlocking
@16:38:39.63 Archive.seek :clear
@16:38:39.67 <Air> Air.in :unlocking
@16:38:39.80 Air.seek :clear
@16:38:39.89 <Lysis1> Lysis1.in :unlocking
@16:38:40.01 Lysis1.seek :clear
@16:38:43.71 <LRAUV> Archive.coast
@16:38:43.76 Air.coast
@16:38:43.81 Lysis1.coast
@16:38:43.86 Lysis2.coast
@16:38:43.92 Plunge.coast
@16:38:43.97 SamplePump.coast
@16:38:44.02 Toroid.coast
@16:38:44.06 Carousel.coast
@16:38:44.11 PinPort.coast
@16:38:44.30 Gate.power :motor,:OFF
@16:38:44.32 Gate.power :core,:OFF
@16:38:44.34 Gate.power :analytic1,:OFF
@16:38:44.36 Gate.power :analytic2,:OFF
@16:38:44.38 Gate.power :analytic3,:OFF
@16:38:44.40 Gate.power :main,:OFF
@16:38:44.42 Safely stopped and ready to power off
##-- file closed 2021-04-25T23:53:23.773Z

##-- file opened 2021-04-25T23:53:23.775Z
@16:53:24.06 -> showStatus
@16:53:26.08 -> Cmd.loadCartridge -9
@16:53:26.12 Cartridge::None in LRAUV -- No dry daSPR_bac cartridges remain
@16:53:28.51 -> Cmd.stop
@16:53:28.58 ready to power off
##-- file closed 2021-04-26T17:53:45.855Z