##-- file opened 2019-08-24T10:08:20.702Z @06:08:21.37 -> showStatus @06:08:23.39 -> Cmd.loadCartridge -4 @06:08:23.53 Gate.power :main,:ON,limit to 5000mA @06:08:23.54 Gate.power :analytic1,:ON,limit to 5000mA @06:08:23.57 Gate.power :analytic2,:ON,limit to 5000mA @06:08:23.59 Gate.power :analytic3,:ON,limit to 5000mA @06:08:23.72 Powering core bus @06:08:26.01 SPR Module 1, FW Rev. B, FPGA Rev. C @06:08:26.13 Archive.configure :default @06:08:26.25 Air.configure :default @06:08:26.36 Lysis1.configure :default @06:08:26.46 Lysis2.configure :default @06:08:26.58 Plunge.configure :default @06:08:26.69 SamplePump.configure :default @06:08:26.81 ExtraPump.configure :default @06:08:26.96 Toroid.configure :default @06:08:27.08 Carousel.configure :default @06:08:27.20 Pressure.configure :default @06:08:27.30 PinPort.configure :default @06:08:27.35 All elf controllers running firmware version 4.58 @06:08:27.38 Gate.power :motor,:ON,limit to 5000mA @06:08:27.54 Archive.reconfigure :default @06:08:27.83 Archive.home.jog 0 @06:08:27.95 PinPort.reconfigure :default @06:08:28.03 Plunge.reconfigure :default @06:08:28.07 Lysis1.reconfigure :default @06:08:28.13 Toroid.reconfigure :default @06:08:28.16 Air.reconfigure :default @06:08:28.21 Lysis2.reconfigure :default @06:08:28.43 Air.home.jog 0 @06:08:28.47 PinPort.home.jog 0 @06:08:28.53 Toroid.home.jog 0 @06:08:28.59 Lysis1.home.jog 0 @06:08:28.65 Lysis2.home.jog 0 @06:08:28.71 Plunge.home.jog 0 @06:08:29.01 Archive.reconfigure :homing @06:08:29.22 Toroid.reconfigure :homing @06:08:29.25 Plunge.reconfigure :homing @06:08:29.28 Air.reconfigure :homing @06:08:29.32 Lysis2.reconfigure :homing @06:08:29.35 Lysis1.reconfigure :homing @06:08:29.39 PinPort.reconfigure :homing @06:08:29.63 Air.jog 1407 @06:08:29.69 Archive.jog 1378 @06:08:29.74 Toroid.jog 623 @06:08:29.80 Lysis1.jog 1397 @06:08:29.86 Plunge.jog 1536 @06:08:29.91 Lysis2.jog 1424 @06:08:29.97 PinPort.jog -106 @06:08:30.20 PinPort.setRawPosition! 0 @06:08:30.24 PinPort.seek :home @06:08:30.33 PinPort.reconfigure :default @06:08:30.79 Air.setRawPosition! 50 @06:08:30.84 Air.seek :home @06:08:30.94 Lysis1.setRawPosition! 50 @06:08:30.96 Archive.setRawPosition! 50 @06:08:31.03 Lysis1.seek :home @06:08:31.08 Archive.seek :home @06:08:31.23 Plunge.setRawPosition! 50 @06:08:31.26 Air.reconfigure :default @06:08:31.29 Lysis2.setRawPosition! 50 @06:08:31.36 Plunge.seek :home @06:08:31.48 Lysis2.seek :home @06:08:31.59 Archive.reconfigure :default @06:08:31.61 Lysis1.reconfigure :default @06:08:31.75 Plunge.reconfigure :default @06:08:31.86 Toroid.setRawPosition! 0 @06:08:31.87 Lysis2.reconfigure :default @06:08:31.94 Toroid.seek :home @06:08:32.06 Toroid.reconfigure :default @06:08:32.32 Toroid.seek :clear @06:08:33.94 Carousel.reconfigure :default @06:08:34.01 Carousel.home.jog 0 @06:08:34.78 Carousel.seek 22 @06:08:34.94 Selecting Cartridge 23 @06:08:35.19 Carousel.seek 23 @06:08:35.67 Lysis1.seek :locked @06:08:39.80 Plunge.seek :locked @06:08:39.92 Air.seek :locked @06:08:44.05 Powering cartridge bus @06:08:46.17 Gate.setIR 1750,5000,0,6 @06:08:46.30 Gate.setIR 0,5000,0,6 @06:08:46.33 Loaded Archive Cartridge @06:08:46.34 Gate.power :cartridge,:OFF @06:09:02.29 -> Cmd.startFiltering @06:09:02.51 Duration of filtering limited to 2:05:00 @06:09:02.77 Priming sample loop w/100ml, bypass w/10ml @06:09:02.78 SamplePump.setPosition! 0ml @06:09:02.83 Bypass.open @06:09:04.33 Intake.open @06:09:05.16 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:09:07.99 Vacuum tare is 52% of full scale @06:09:08.12 Pressure.reconfigure :default @06:09:08.18 Pressure.hold @06:09:08.34 SamplePump.reconfigure :prime @06:09:08.42 SamplePump.seek 10ml,1:21 @06:09:17.37 Pressure.coast @06:09:17.42 SamplePump.setPosition! 0ml @06:09:17.47 Exhaust.open @06:09:18.91 Bypass.close @06:09:20.50 Pressure.reconfigure :default @06:09:20.56 Pressure.hold @06:09:20.78 SamplePump.reconfigure :prime @06:09:20.85 SamplePump.seek 100ml,8:51 @06:11:17.31 Pressure.coast @06:11:17.37 Bypass.open @06:11:18.10 Intake.close @06:11:18.96 Toroid.reconfigure :hold @06:11:19.02 Toroid.seek :crack @06:11:19.82 Toroid.reconfigure :default @06:11:19.88 Toroid.seek :clear @06:11:21.44 Toroid.reconfigure :hold @06:11:21.51 Toroid.seek :cartridge @06:11:23.33 Toroid.reconfigure :default @06:11:23.39 Toroid.seek :clear @06:11:25.36 Pressurizing cartridge to 26psi above 10.1psia @06:11:25.38 SamplePump.setPosition! 0ml @06:11:25.42 Intake.open @06:11:26.18 Bypass.close @06:11:26.88 Exhaust.close @06:11:27.70 Pressure.reconfigure :default @06:11:27.75 Pressure.hold @06:11:27.92 SamplePump.reconfigure :charge @06:11:27.99 SamplePump.seek 2.3ml,42 seconds @06:11:37.77 Pressure.coast @06:11:37.83 Intake.close @06:11:38.66 Pressurized cartridge with 0.448ml to 26.2psi Isolated @33.5psia (23.4psi) @06:11:38.68 Checking for cartridge leaks from 33.5psia @06:11:38.71 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @06:11:43.75 Cartridge pressure fell 1% to 33.2psia @06:11:43.83 Sampling 1000.0ml @06:11:43.84 SamplePump.setPosition! 0ml @06:11:43.89 Exhaust.open @06:11:44.60 Intake.open @06:11:45.48 Pressure.reconfigure :default @06:11:45.54 Pressure.hold @06:11:45.75 SamplePump.reconfigure :sample @06:11:45.83 SamplePump.seek 1000ml,1:23:53 @06:11:48.52 Cartridge 23 sampling 1/1000ml at 0.40ml/s with 24.7psi vacuum @06:12:48.52 Cartridge 23 sampling 23/1000ml at 0.34ml/s with 26.5psi vacuum @06:13:48.52 Cartridge 23 sampling 39/1000ml at 0.25ml/s with 25.0psi vacuum @06:14:48.52 Cartridge 23 sampling 52/1000ml at 0.20ml/s with 24.8psi vacuum @06:15:48.52 Cartridge 23 sampling 64/1000ml at 0.20ml/s with 28.7psi vacuum @06:15:55.68 Flow averaged 0.27ml/s over 4:03 @06:15:55.71 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi @06:15:56.40 Relieved excess vacuum in 7.8 seconds @06:15:56.43 SamplePump.seek 1000ml,1:18:23 @06:16:04.86 Flow estimate reduced to 0.22ml/s @06:16:04.88 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @06:16:05.59 Relieved excess vacuum in 6.1 seconds @06:16:05.61 SamplePump.seek 1000ml,1:18:15 @06:16:10.54 Flow estimate reduced to 0.17ml/s @06:16:10.57 Intake.close @06:16:10.62 Exhaust.close @06:16:10.79 Toroid.reconfigure :hold @06:16:10.87 Toroid.seek :bypass @06:16:12.49 Toroid.reconfigure :default @06:16:12.56 Toroid.seek :clear @06:16:14.18 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @06:16:14.20 Pressure.coast @06:16:14.38 Sampled 68.4ml @06:16:16.59 -> Cmd.startProcessing @06:16:16.84 Plunge.seek :reagent @06:16:17.70 Sample loop closed. Ready to Deliver Reagents @06:16:17.71 Archive.seek :sealed @06:16:21.98 Archive.seek :evac @06:16:35.90 delay 1:00 @06:17:35.93 Archive.seek :clear @06:17:53.62 RNAlater soak for 10:00 @06:27:53.65 Archive.seek :tVent @06:27:56.22 delay 1 second @06:27:57.24 Archive.seek :evac @06:28:12.84 delay 10 seconds @06:28:22.86 Archive.seek :tVent @06:28:38.47 delay 1 second @06:28:39.49 Archive.seek :evac @06:28:55.09 delay 10 seconds @06:29:05.12 Sample preserved and evacuated @06:29:06.22 -> Cmd.stop @06:29:06.36 Carousel.seek 23 @06:29:06.53 Plunge.seek :locked @06:29:07.37 PinPort.seek :clear @06:29:07.51 Archive.seek :clear @06:29:07.64 Air.seek :clear @06:29:07.77 Lysis1.seek :clear @06:29:25.24 Archive.coast @06:29:25.29 Air.coast @06:29:25.34 Lysis1.coast @06:29:25.39 Lysis2.coast @06:29:25.44 Plunge.coast @06:29:25.49 SamplePump.coast @06:29:25.54 Toroid.coast @06:29:25.59 Carousel.coast @06:29:25.65 PinPort.coast @06:29:25.83 Gate.power :motor,:OFF @06:29:25.84 Gate.power :core,:OFF @06:29:25.87 Gate.power :analytic1,:OFF @06:29:25.89 Gate.power :analytic2,:OFF @06:29:25.91 Gate.power :analytic3,:OFF @06:29:25.93 Gate.power :main,:OFF @06:29:25.95 Safely stopped and ready to power off ##-- file closed 2019-08-24T10:45:14.299Z ##-- file opened 2019-08-24T10:45:14.301Z @06:45:14.33 -> showStatus @06:45:16.34 -> Cmd.loadCartridge -15 @06:45:16.48 Gate.power :main,:ON,limit to 5000mA @06:45:16.49 Gate.power :analytic1,:ON,limit to 5000mA @06:45:16.52 Gate.power :analytic2,:ON,limit to 5000mA @06:45:16.54 Gate.power :analytic3,:ON,limit to 5000mA @06:45:16.68 Powering core bus @06:45:18.95 SPR Module 1, FW Rev. B, FPGA Rev. C @06:45:19.08 Archive.configure :default @06:45:19.20 Air.configure :default @06:45:19.30 Lysis1.configure :default @06:45:19.40 Lysis2.configure :default @06:45:19.52 Plunge.configure :default @06:45:19.64 SamplePump.configure :default @06:45:19.76 ExtraPump.configure :default @06:45:19.88 Toroid.configure :default @06:45:20.03 Carousel.configure :default @06:45:20.15 Pressure.configure :default @06:45:20.25 PinPort.configure :default @06:45:20.30 All elf controllers running firmware version 4.58 @06:45:20.33 Gate.power :motor,:ON,limit to 5000mA @06:45:20.53 Archive.reconfigure :default @06:45:20.76 Archive.home.jog 0 @06:45:20.92 PinPort.reconfigure :default @06:45:20.97 Toroid.reconfigure :default @06:45:21.01 Lysis2.reconfigure :default @06:45:21.05 Air.reconfigure :default @06:45:21.09 Lysis1.reconfigure :default @06:45:21.19 Plunge.reconfigure :default @06:45:21.39 Air.home.jog 0 @06:45:21.44 Plunge.home.jog 0 @06:45:21.50 Toroid.home.jog 0 @06:45:21.56 PinPort.home.jog 0 @06:45:21.61 Lysis1.home.jog 0 @06:45:21.67 Lysis2.home.jog 0 @06:45:21.98 Archive.reconfigure :homing @06:45:22.21 Toroid.reconfigure :homing @06:45:22.23 Plunge.reconfigure :homing @06:45:22.26 Air.reconfigure :homing @06:45:22.30 PinPort.reconfigure :homing @06:45:22.33 Lysis1.reconfigure :homing @06:45:22.37 Lysis2.reconfigure :homing @06:45:22.61 Lysis1.jog 1399 @06:45:22.66 Archive.jog 1378 @06:45:22.72 Toroid.jog 623 @06:45:22.78 Air.jog 1396 @06:45:22.84 Plunge.jog 1529 @06:45:22.89 Lysis2.jog 1420 @06:45:22.95 PinPort.jog -108 @06:45:23.18 PinPort.setRawPosition! 0 @06:45:23.22 PinPort.seek :home @06:45:23.31 PinPort.reconfigure :default @06:45:23.76 Lysis1.setRawPosition! 50 @06:45:23.82 Lysis1.seek :home @06:45:23.89 Archive.setRawPosition! 50 @06:45:23.95 Archive.seek :home @06:45:24.06 Air.setRawPosition! 50 @06:45:24.11 Air.seek :home @06:45:24.20 Plunge.setRawPosition! 50 @06:45:24.23 Lysis2.setRawPosition! 50 @06:45:24.26 Lysis1.reconfigure :default @06:45:24.36 Plunge.seek :home @06:45:24.41 Lysis2.seek :home @06:45:24.58 Air.reconfigure :default @06:45:24.60 Archive.reconfigure :default @06:45:24.76 Plunge.reconfigure :default @06:45:24.84 Toroid.setRawPosition! 0 @06:45:24.86 Lysis2.reconfigure :default @06:45:24.94 Toroid.seek :home @06:45:25.06 Toroid.reconfigure :default @06:45:25.28 Toroid.seek :clear @06:45:27.23 Carousel.reconfigure :default @06:45:27.34 Carousel.home.jog 0 @06:45:27.83 Carousel.seek 23 @06:45:28.00 Selecting Cartridge 20 @06:45:28.24 Carousel.seek 20 @06:45:29.13 Lysis1.seek :locked @06:45:33.26 Plunge.seek :locked @06:45:33.38 Air.seek :locked @06:45:37.51 Powering cartridge bus @06:45:39.64 Gate.setIR 1750,5000,0,6 @06:45:39.76 Gate.setIR 0,5000,0,6 @06:45:39.81 Loaded Lyse-n-Go Cartridge @06:45:39.83 PuckHeater.configure HeatConfig @06:45:39.87 Gate.power :heat,:ON,limit to 5000mA @06:45:55.78 -> Cmd.startFiltering @06:45:56.01 Duration of filtering limited to 2:05:00 @06:45:56.27 Priming sample loop w/100ml, bypass w/10ml @06:45:56.29 SamplePump.setPosition! 0ml @06:45:56.33 Bypass.open @06:45:57.82 Intake.open @06:45:58.63 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:46:01.46 Vacuum tare is 52% of full scale @06:46:01.59 Pressure.reconfigure :default @06:46:01.65 Pressure.hold @06:46:01.81 SamplePump.reconfigure :prime @06:46:01.88 SamplePump.seek 10ml,1:21 @06:46:10.84 Pressure.coast @06:46:10.88 SamplePump.setPosition! 0ml @06:46:10.93 Exhaust.open @06:46:12.39 Bypass.close @06:46:13.97 Pressure.reconfigure :default @06:46:14.03 Pressure.hold @06:46:14.24 SamplePump.reconfigure :prime @06:46:14.32 SamplePump.seek 100ml,8:51 @06:48:11.00 Pressure.coast @06:48:11.06 Bypass.open @06:48:11.80 Intake.close @06:48:12.64 Toroid.reconfigure :hold @06:48:12.70 Toroid.seek :crack @06:48:13.50 Toroid.reconfigure :default @06:48:13.56 Toroid.seek :clear @06:48:15.12 Toroid.reconfigure :hold @06:48:15.19 Toroid.seek :cartridge @06:48:17.00 Toroid.reconfigure :default @06:48:17.06 Toroid.seek :clear @06:48:19.04 Pressurizing cartridge to 26psi above 10.4psia @06:48:19.05 SamplePump.setPosition! 0ml @06:48:19.09 Intake.open @06:48:19.86 Bypass.close @06:48:20.60 Exhaust.close @06:48:21.42 Pressure.reconfigure :default @06:48:21.47 Pressure.hold @06:48:21.64 SamplePump.reconfigure :charge @06:48:21.71 SamplePump.seek 2.3ml,42 seconds @06:48:34.53 Pressure.coast @06:48:34.59 Intake.close @06:48:35.41 Pressurized cartridge with 0.773ml to 26.1psi @06:48:35.42 Isolated @33.3psia (22.9psi) @06:48:35.43 Checking for cartridge leaks from 33.3psia @06:48:35.46 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @06:48:40.56 Sampling 1000.0ml @06:48:40.57 SamplePump.setPosition! 0ml @06:48:40.62 Exhaust.open @06:48:41.33 Intake.open @06:48:42.19 Pressure.reconfigure :default @06:48:42.25 Pressure.hold @06:48:42.47 SamplePump.reconfigure :sample @06:48:42.54 SamplePump.seek 1000ml,1:23:53 @06:48:45.22 Cartridge 20 sampling 1/1000ml at 0.50ml/s with 22.8psi vacuum @06:49:45.23 Cartridge 20 sampling 32/1000ml at 0.50ml/s with 24.5psi vacuum @06:50:45.23 Cartridge 20 sampling 61/1000ml at 0.46ml/s with 22.8psi vacuum @06:51:45.23 Cartridge 20 sampling 87/1000ml at 0.41ml/s with 22.0psi vacuum @06:52:45.23 Cartridge 20 sampling 111/1000ml at 0.39ml/s with 21.5psi vacuum @06:53:45.23 Cartridge 20 sampling 133/1000ml at 0.35ml/s with 21.4psi vacuum 1.9m deep (17.6psia) @06:54:45.23 Cartridge 20 sampling 153/1000ml at 0.27ml/s with 23.4psi vacuum @06:55:45.60 Cartridge 20 sampling 170/1000ml at 0.28ml/s with 24.2psi vacuum @06:56:45.23 Cartridge 20 sampling 184/1000ml at 0.21ml/s with 24.0psi vacuum @06:57:45.23 Cartridge 20 sampling 197/1000ml at 0.21ml/s with 22.4psi vacuum @06:58:45.23 Cartridge 20 sampling 209/1000ml at 0.20ml/s with 27.1psi vacuum @06:59:22.76 Flow averaged 0.34ml/s over 10:33 @06:59:22.79 Waiting up to 13 seconds for vacuum to reduce from 27.8 to 18.0psi @06:59:23.49 Relieved excess vacuum in 8.1 seconds @06:59:23.51 SamplePump.seek 1000ml,1:05:48 @06:59:45.23 Cartridge 20 sampling 221/1000ml at 0.20ml/s with 27.2psi vacuum @06:59:45.94 Flow averaged 0.21ml/s over 22 seconds @06:59:45.97 Waiting up to 14 seconds for vacuum to reduce from 28.5 to 18.0psi @06:59:46.67 Relieved excess vacuum in 1.4 seconds @06:59:46.69 SamplePump.seek 1000ml,1:05:26 @06:59:54.85 Flow estimate reduced to 0.16ml/s @06:59:54.87 Intake.close @06:59:54.93 Exhaust.close @06:59:55.10 Toroid.reconfigure :hold @06:59:55.18 Toroid.seek :bypass @06:59:56.79 Toroid.reconfigure :default @06:59:56.86 Toroid.seek :clear @06:59:58.48 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @06:59:58.50 Pressure.coast @06:59:58.68 Sampled 222.9ml @07:00:00.89 -> Cmd.startProcessing @07:00:02.75 SPR.command \"read_sequences\" @07:00:03.20 SPR.command \"stats_on\" @07:00:03.24 SPR.command \"start 0 1\" @07:00:05.24 Begun log /var/log/esp/spr19aug2407h.spr @07:00:06.27 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @07:00:07.94 Handoff.select :waste @07:00:08.11 Archive.seek :empty @07:00:15.31 Syringe tare is 21% of full scale @07:00:25.83 PinPort.seek :cartridge @07:00:30.39 Lysis1.seek 1350ul @07:00:32.87 Lysis2.seek 1700ul @07:00:35.97 Plunge.seek :reagent @07:00:36.81 Evacuating puck with filter bubble point of 50psi @07:00:36.84 PinPort.seek :cartridge @07:00:36.92 Handoff.select :waste @07:00:44.24 Air pumping 1 strokes to reach 8psi @07:00:44.26 Air.reconfigure :gulp @07:00:44.34 Air.seek :tVent @07:00:46.15 0.2psi after gulp @07:00:46.16 Air stroke #1 of 1 @07:00:46.17 Air.reconfigure :pressurize @07:00:46.27 Air.seek :pressurized @07:00:59.10 0.2psi after pressurize @07:00:59.11 Air.reconfigure :compress @07:00:59.19 Air.seek :evac @07:01:01.85 0.5psi after compress @07:01:15.75 0.2psi after compress (stable) @07:01:15.87 at 0.1psi Opened to waste @07:01:15.88 Handoff.select :blocked @07:01:16.05 Air pumping 10 strokes to reach 50psi @07:01:16.07 Air.reconfigure :gulp @07:01:16.15 Air.seek :tVent @07:01:26.05 9.5psi after gulp @07:01:26.06 Air stroke #1 of 10 @07:01:26.08 Air.reconfigure :pressurize @07:01:26.16 Air.seek :pressurized @07:01:39.00 12.9psi after pressurize @07:01:39.02 Air.reconfigure :compress @07:01:39.10 Air.seek :evac @07:01:41.76 15.1psi after compress @07:02:11.13 34.3psi after compress (stable) @07:02:11.14 Air.reconfigure :gulp @07:02:11.22 Air.seek :tVent @07:02:21.11 35.2psi after gulp @07:02:21.13 Air stroke #2 of 10 @07:02:21.15 Air.reconfigure :pressurize @07:02:21.23 Air.seek :pressurized @07:02:34.08 33.8psi after pressurize @07:02:34.11 Air.reconfigure :compress @07:02:34.19 Air.seek :evac @07:02:36.86 33.7psi after compress @07:03:03.51 49.6psi after compress (stable) @07:03:03.53 Air.reconfigure :gulp @07:03:03.60 Air.seek :tVent @07:03:03.79 Reached 49.8 of 50.0psi after pumping 3115ul (2.00 strokes) @07:03:03.80 Handoff.select :waste @07:03:36.93 delay 30 seconds @07:04:06.95 Handoff.select :blocked @07:04:31.97 Handoff.select :waste @07:04:32.09 PSI at @2.12psi at evacEnd @07:04:32.10 Handoff.select :blocked @07:04:32.22 Air.seek :tVent @07:04:49.29 Stable @0.4psi after Handoff blocked @07:04:49.39 Air pumping 10 strokes to reach 30psi @07:04:49.40 Air stroke #1 of 10 @07:04:49.43 Air.reconfigure :pressurize @07:04:49.51 Air.seek :pressurized @07:05:02.35 2.0psi after pressurize @07:05:02.36 Air.reconfigure :compress @07:05:02.44 Air.seek :evac @07:05:05.10 4.9psi after compress @07:05:27.16 13.9psi after compress (stable) @07:05:27.19 Air.reconfigure :gulp @07:05:27.27 Air.seek :tVent @07:05:37.15 14.4psi after gulp @07:05:37.16 Air stroke #2 of 10 @07:05:37.18 Air.reconfigure :pressurize @07:05:37.26 Air.seek :pressurized @07:05:50.09 14.5psi after pressurize @07:05:50.11 Air.reconfigure :compress @07:05:50.18 Air.seek :evac @07:05:52.85 16.5psi after compress @07:06:07.33 21.1psi after compress (stable) @07:06:07.35 Air.reconfigure :gulp @07:06:07.43 Air.seek :tVent @07:06:17.34 23.6psi after gulp @07:06:17.35 Air stroke #3 of 10 @07:06:17.38 Air.reconfigure :pressurize @07:06:17.46 Air.seek :pressurized @07:06:30.29 24.3psi after pressurize @07:06:30.30 Air.reconfigure :compress @07:06:30.38 Air.seek :evac @07:06:33.05 25.3psi after compress @07:06:36.48 Reached 30.4 of 30.4psi after pumping 6230ul (4.00 strokes) @07:06:36.50 Handoff.select :waste @07:06:54.32 delay 30 seconds @07:07:24.35 Handoff.select :blocked @07:07:49.37 Handoff.select :waste @07:07:49.49 PSI at @1.22psi at evacEnd @07:07:49.50 Air.reconfigure :default @07:07:49.62 Air.seek :fVent @07:07:51.07 delay 5 seconds @07:07:56.09 Seawater evacuated, Presure above/below filter released. Lysis begins @07:07:56.17 Lysis1.reconfigure :slow @07:07:56.24 Lysis1.seek 1050ul @07:08:26.25 delay 5 seconds @07:08:31.27 Handoff.select :blocked @07:08:31.43 Lysis1.seek 450ul @07:09:31.37 delay 3 seconds @07:09:34.44 Air.reconfigure to mix into 1.37806 parts Lysis1 @07:09:34.92 Air.seek :evac @07:09:34.97 Lysis1.seek 300ul @07:09:50.09 Air.reconfigure :default @07:09:50.18 Air.seek :full @07:10:04.39 Lysis1.seek :empty @07:10:34.48 Lysis Delivered. Syringe pressure @22.5psi @07:10:34.53 PuckHeater.seek 84.3C @07:11:24.10 PuckHeater.hold 15:00,84.3C @07:26:20.91 Lysis complete. Syringe pressure @29.8psi @07:26:20.92 Handoff.select :blocked @07:26:21.02 Lysis1.reconfigure :halfSpeed @07:26:21.09 Lysis1.seek 200ul @07:26:24.85 delay 1 second @07:26:25.87 Lysis1.seek :empty @07:26:29.70 Initial AirS pressure @29.5psi @07:26:29.76 Lysis1.reconfigure :extract @07:26:29.85 Lysis1.seek 900ul,10:00 @07:26:31.11 Air pumping 10 strokes to reach 21psi @07:26:31.12 Air stroke #1 of 10 @07:26:31.13 Air.reconfigure :pressurize @07:26:31.22 Air.seek :pressurized @07:26:44.06 15.7psi after pressurize @07:26:44.08 Air.reconfigure :compress @07:26:44.16 Air.seek :evac @07:26:46.83 16.8psi after compress @07:27:01.15 15.8psi after compress (stable) @07:27:01.17 Air.reconfigure :gulp @07:27:01.25 Air.seek :tVent @07:27:11.13 15.3psi after gulp @07:27:11.14 Air stroke #2 of 10 @07:27:11.16 Air.reconfigure :pressurize @07:27:11.24 Air.seek :pressurized @07:27:24.06 15.8psi after pressurize @07:27:24.07 Air.reconfigure :compress @07:27:24.15 Air.seek :evac @07:27:26.82 15.6psi after compress @07:27:37.32 Air.reconfigure :default @07:27:37.38 Air.hold @07:27:37.55 Lysis1 at 900ul @15.7psi @07:27:37.57 Lysis1.reconfigure :default @07:27:37.64 Lysis1.seek 925ul @07:27:38.36 Syringe::Overpressure in PROCESSING -- 16.7psi after recovery pull -- Skipping SPR @07:27:38.38 SPR.command \"start 2 14\" @07:27:39.36 Handoff.select :waste @07:27:39.84 Ended log /var/log/esp/spr19aug2407h.spr @07:27:39.85 SPR.command \"stats_off\" @07:27:42.21 -> Cmd.stop @07:27:42.30 Handoff.select :close @07:27:42.43 Gate.power :heat,:OFF @07:27:42.45 Gate.power :cartridge,:OFF @07:27:42.53 Carousel.seek 20 @07:27:42.70 Plunge.seek :locked @07:27:43.54 PinPort.seek :clear @07:27:48.15 Archive.seek :clear @07:27:48.28 Air.seek :clear @07:27:48.41 Lysis1.seek :clear @07:27:48.54 Lysis2.seek :clear @07:28:05.90 Archive.coast @07:28:05.95 Air.coast @07:28:06.00 Lysis1.coast @07:28:06.05 Lysis2.coast @07:28:06.11 Plunge.coast @07:28:06.15 SamplePump.coast @07:28:06.20 Toroid.coast @07:28:06.25 Carousel.coast @07:28:06.31 PinPort.coast @07:28:06.49 Gate.power :motor,:OFF @07:28:06.50 Gate.power :core,:OFF @07:28:06.53 Gate.power :analytic1,:OFF @07:28:06.55 Gate.power :analytic2,:OFF @07:28:06.57 Gate.power :analytic3,:OFF @07:28:06.59 Gate.power :main,:OFF @07:28:06.61 Safely stopped and ready to power off ##-- file closed 2019-08-24T13:34:07.167Z ##-- file opened 2019-08-24T13:34:07.168Z @09:34:07.53 -> showStatus @09:34:09.55 -> Cmd.loadCartridge -4 @09:34:09.69 Gate.power :main,:ON,limit to 5000mA @09:34:09.70 Gate.power :analytic1,:ON,limit to 5000mA @09:34:09.72 Gate.power :analytic2,:ON,limit to 5000mA @09:34:09.75 Gate.power :analytic3,:ON,limit to 5000mA @09:34:09.88 Powering core bus @09:34:12.18 SPR Module 1, FW Rev. B, FPGA Rev. C @09:34:12.30 Archive.configure :default @09:34:12.42 Air.configure :default @09:34:12.52 Lysis1.configure :default @09:34:12.62 Lysis2.configure :default @09:34:12.73 Plunge.configure :default @09:34:12.85 SamplePump.configure :default @09:34:12.97 ExtraPump.configure :default @09:34:13.09 Toroid.configure :default @09:34:13.21 Carousel.configure :default @09:34:13.33 Pressure.configure :default @09:34:13.43 PinPort.configure :default @09:34:13.49 All elf controllers running firmware version 4.58 @09:34:13.51 Gate.power :motor,:ON,limit to 5000mA @09:34:13.73 Archive.reconfigure :default @09:34:13.97 Archive.home.jog 0 @09:34:14.10 Toroid.reconfigure :default @09:34:14.18 Plunge.reconfigure :default @09:34:14.22 Lysis1.reconfigure :default @09:34:14.26 PinPort.reconfigure :default @09:34:14.30 Air.reconfigure :default @09:34:14.39 Lysis2.reconfigure :default @09:34:14.58 Air.home.jog 0 @09:34:14.63 Lysis1.home.jog 0 @09:34:14.69 PinPort.home.jog 0 @09:34:14.75 Plunge.home.jog 0 @09:34:14.81 Toroid.home.jog 0 @09:34:14.87 Lysis2.home.jog 0 @09:34:15.17 Archive.reconfigure :homing @09:34:15.28 Archive.jog 1377 @09:34:15.46 Toroid.reconfigure :homing @09:34:15.48 Plunge.reconfigure :homing @09:34:15.51 Air.reconfigure :homing @09:34:15.55 PinPort.reconfigure :homing @09:34:15.58 Lysis2.reconfigure :homing @09:34:15.62 Lysis1.reconfigure :homing @09:34:15.85 Lysis2.jog 1425 @09:34:15.90 Toroid.jog 626 @09:34:15.96 Plunge.jog 1532 @09:34:16.02 PinPort.jog -105 @09:34:16.08 Lysis1.jog 1400 @09:34:16.14 Air.jog 1405 @09:34:16.25 PinPort.setRawPosition! 0 @09:34:16.30 PinPort.seek :home @09:34:16.38 PinPort.reconfigure :default @09:34:16.46 Archive.setRawPosition! 50 @09:34:16.51 Archive.seek :home @09:34:16.79 Archive.reconfigure :default @09:34:17.02 Lysis2.setRawPosition! 50 @09:34:17.07 Lysis2.seek :home @09:34:17.18 Plunge.setRawPosition! 50 @09:34:17.23 Plunge.seek :home @09:34:17.35 Lysis1.setRawPosition! 50 @09:34:17.37 Air.setRawPosition! 50 @09:34:17.39 Lysis2.reconfigure :default @09:34:17.52 Lysis1.seek :home @09:34:17.56 Air.seek :home @09:34:17.69 Plunge.reconfigure :default @09:34:17.88 Air.reconfigure :default @09:34:17.96 Lysis1.reconfigure :default @09:34:18.03 Toroid.setRawPosition! 0 @09:34:18.09 Toroid.seek :home @09:34:18.17 Toroid.reconfigure :default @09:34:18.36 Toroid.seek :clear @09:34:20.01 Carousel.reconfigure :default @09:34:20.08 Carousel.home.jog 0 @09:34:20.58 Carousel.seek 20 @09:34:20.75 Selecting Cartridge 21 @09:34:20.99 Carousel.seek 21 @09:34:21.48 Lysis1.seek :locked @09:34:25.61 Plunge.seek :locked @09:34:25.73 Air.seek :locked @09:34:29.90 Powering cartridge bus @09:34:32.04 Gate.setIR 1750,5000,0,6 @09:34:32.17 Gate.setIR 0,5000,0,6 @09:34:32.21 Loaded Archive Cartridge Gate.power :cartridge,:OFF @09:34:48.46 -> Cmd.startFiltering @09:34:48.69 Duration of filtering limited to 2:05:00 @09:34:48.94 Priming sample loop w/100ml, bypass w/10ml @09:34:48.95 SamplePump.setPosition! 0ml @09:34:49.01 Bypass.open @09:34:50.51 Intake.open @09:34:51.30 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:34:54.14 Vacuum tare is 52% of full scale @09:34:54.27 Pressure.reconfigure :default @09:34:54.33 Pressure.hold @09:34:54.50 SamplePump.reconfigure :prime @09:34:54.57 SamplePump.seek 10ml,1:21 @09:35:03.53 Pressure.coast @09:35:03.58 SamplePump.setPosition! 0ml @09:35:03.63 Exhaust.open @09:35:05.03 Bypass.close @09:35:06.59 Pressure.reconfigure :default @09:35:06.65 Pressure.hold @09:35:06.87 SamplePump.reconfigure :prime @09:35:06.94 SamplePump.seek 100ml,8:51 @09:37:03.18 Pressure.coast @09:37:03.24 Bypass.open @09:37:03.99 Intake.close @09:37:04.83 Toroid.reconfigure :hold @09:37:04.89 Toroid.seek :crack @09:37:05.69 Toroid.reconfigure :default @09:37:05.75 Toroid.seek :clear @09:37:07.31 Toroid.reconfigure :hold @09:37:07.38 Toroid.seek :cartridge @09:37:09.20 Toroid.reconfigure :default @09:37:09.26 Toroid.seek :clear @09:37:11.24 Pressurizing cartridge to 26psi above 13.7psia @09:37:11.25 SamplePump.setPosition! 0ml @09:37:11.29 Intake.open @09:37:12.07 Bypass.close @09:37:12.81 Exhaust.close @09:37:13.63 Pressure.reconfigure :default @09:37:13.68 Pressure.hold @09:37:13.85 SamplePump.reconfigure :charge @09:37:13.92 SamplePump.seek 2.3ml,42 seconds @09:37:21.85 Pressure.coast @09:37:21.90 Intake.close @09:37:22.75 Pressurized cartridge with 0.292ml to 26.0psi @09:37:22.76 Isolated @36.3psia (22.6psi) @09:37:22.77 Checking for cartridge leaks from 36.3psia @09:37:22.80 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @09:37:27.85 Cartridge pressure fell 1% to 36.1psia @09:37:27.93 Sampling 1000.0ml @09:37:27.94 SamplePump.setPosition! 0ml @09:37:27.99 Exhaust.open @09:37:28.73 Intake.open @09:37:29.58 Pressure.reconfigure :default @09:37:29.64 Pressure.hold @09:37:29.86 SamplePump.reconfigure :sample @09:37:29.94 SamplePump.seek 1000ml,1:23:53 @09:37:32.63 Cartridge 21 sampling 1/1000ml at 0.41ml/s with 22.2psi vacuum @09:38:32.62 Cartridge 21 sampling 23/1000ml at 0.34ml/s with 25.9psi vacuum @09:39:32.62 Cartridge 21 sampling 42/1000ml at 0.24ml/s with 26.6psi vacuum @09:40:32.62 Cartridge 21 sampling 56/1000ml at 0.20ml/s with 26.8psi vacuum @09:40:58.49 Flow averaged 0.30ml/s over 3:23 @09:40:58.52 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi @09:40:59.23 Relieved excess vacuum in 6.5 seconds @09:40:59.25 SamplePump.seek 1000ml,1:18:48 @09:41:10.53 Flow estimate reduced to 0.24ml/s @09:41:10.57 Waiting up to 13 seconds for vacuum to reduce from 27.8 to 18.0psi @09:41:11.57 Relieved excess vacuum in 9.2 seconds @09:41:11.59 SamplePump.seek 1000ml,1:18:37 @09:41:13.46 Flow estimate reduced to 0.19ml/s @09:41:13.48 Intake.close @09:41:13.55 Exhaust.close @09:41:13.71 Toroid.reconfigure :hold @09:41:13.78 Toroid.seek :bypass @09:41:15.40 Toroid.reconfigure :default @09:41:15.47 Toroid.seek :clear @09:41:17.10 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @09:41:17.12 Pressure.coast @09:41:17.30 Sampled 63.4ml @09:41:19.30 -> Cmd.startProcessing @09:41:19.54 Plunge.seek :reagent @09:41:20.40 Sample loop closed. Ready to Deliver Reagents @09:41:20.41 Archive.seek :sealed @09:41:24.69 Archive.seek :evac @09:41:38.64 delay 1:00 @09:42:38.67 Archive.seek :clear @09:42:56.40 RNAlater soak for 10:00 @09:52:56.42 Archive.seek :tVent @09:52:59.02 delay 1 second @09:53:00.04 Archive.seek :evac @09:53:15.66 delay 10 seconds @09:53:25.69 Archive.seek :tVent @09:53:41.31 delay 1 second @09:53:42.33 Archive.seek :evac @09:53:57.95 delay 10 seconds @09:54:07.97 Sample preserved and evacuated @09:54:09.16 -> Cmd.stop @09:54:09.30 Carousel.seek 21 @09:54:09.47 Plunge.seek :locked @09:54:10.31 PinPort.seek :clear @09:54:10.45 Archive.seek :clear @09:54:10.58 Air.seek :clear @09:54:10.71 Lysis1.seek :clear @09:54:28.20 Archive.coast @09:54:28.25 Air.coast @09:54:28.30 Lysis1.coast @09:54:28.35 Lysis2.coast @09:54:28.40 Plunge.coast @09:54:28.45 SamplePump.coast @09:54:28.51 Toroid.coast @09:54:28.56 Carousel.coast @09:54:28.61 PinPort.coast @09:54:28.79 Gate.power :motor,:OFF @09:54:28.80 Gate.power :core,:OFF @09:54:28.83 Gate.power :analytic1,:OFF @09:54:28.85 Gate.power :analytic2,:OFF @09:54:28.87 Gate.power :analytic3,:OFF @09:54:28.89 Gate.power :main,:OFF @09:54:28.91 Safely stopped and ready to power off ##-- file closed 2019-08-24T14:10:16.820Z ##-- file opened 2019-08-24T14:10:16.821Z @10:10:17.55 -> showStatus @10:10:19.60 -> Cmd.loadCartridge -15 @10:10:19.74 Gate.power :main,:ON,limit to 5000mA @10:10:19.75 Gate.power :analytic1,:ON,limit to 5000mA @10:10:19.78 Gate.power :analytic2,:ON,limit to 5000mA @10:10:19.80 Gate.power :analytic3,:ON,limit to 5000mA @10:10:19.93 Powering core bus @10:10:22.21 SPR Module 1, FW Rev. B, FPGA Rev. C @10:10:22.34 Archive.configure :default @10:10:22.46 Air.configure :default @10:10:22.56 Lysis1.configure :default @10:10:22.66 Lysis2.configure :default @10:10:22.78 Plunge.configure :default @10:10:22.90 SamplePump.configure :default @10:10:23.02 ExtraPump.configure :default @10:10:23.14 Toroid.configure :default @10:10:23.26 Carousel.configure :default @10:10:23.38 Pressure.configure :default @10:10:23.49 PinPort.configure :default @10:10:23.55 All elf controllers running firmware version 4.58 @10:10:23.57 Gate.power :motor,:ON,limit to 5000mA @10:10:23.74 Archive.reconfigure :default @10:10:24.02 Archive.home.jog 0 @10:10:24.24 PinPort.reconfigure :default @10:10:24.27 Toroid.reconfigure :default @10:10:24.31 Lysis1.reconfigure :default @10:10:24.34 Air.reconfigure :default @10:10:24.38 Lysis2.reconfigure :default @10:10:24.43 Plunge.reconfigure :default @10:10:24.68 Lysis2.home.jog 0 @10:10:24.73 Toroid.home.jog 0 @10:10:24.79 Lysis1.home.jog 0 @10:10:24.85 Air.home.jog 0 @10:10:24.91 Plunge.home.jog 0 @10:10:24.97 PinPort.home.jog 0 @10:10:25.22 Archive.reconfigure :homing @10:10:25.53 Toroid.reconfigure :homing @10:10:25.57 Plunge.reconfigure :homing @10:10:25.60 PinPort.reconfigure :homing @10:10:25.64 Lysis1.reconfigure :homing @10:10:25.67 Air.reconfigure :homing @10:10:25.71 Lysis2.reconfigure :homing @10:10:25.94 PinPort.jog -106 @10:10:26.00 Archive.jog 1379 @10:10:26.05 Toroid.jog 627 @10:10:26.10 Air.jog 1402 @10:10:26.17 Lysis2.jog 1427 @10:10:26.23 Lysis1.jog 1399 @10:10:26.29 Plunge.jog 1544 @10:10:26.36 PinPort.setRawPosition! 0 @10:10:26.40 PinPort.seek :home @10:10:26.49 PinPort.reconfigure :default @10:10:27.14 Archive.setRawPosition! 50 @10:10:27.18 Archive.seek :home @10:10:27.28 Air.setRawPosition! 50 @10:10:27.34 Air.seek :home @10:10:27.43 Lysis2.setRawPosition! 50 @10:10:27.44 Lysis1.setRawPosition! 50 @10:10:27.53 Lysis2.seek :home @10:10:27.57 Lysis1.seek :home @10:10:27.72 Plunge.setRawPosition! 50 @10:10:27.75 Air.reconfigure :default @10:10:27.77 Archive.reconfigure :default @10:10:27.89 Plunge.seek :home @10:10:28.03 Lysis1.reconfigure :default @10:10:28.05 Lysis2.reconfigure :default @10:10:28.18 Toroid.setRawPosition! 0 @10:10:28.24 Toroid.seek :home @10:10:28.35 Toroid.reconfigure :default @10:10:28.38 Plunge.reconfigure :default @10:10:28.88 Toroid.seek :clear @10:10:30.53 Carousel.reconfigure :default @10:10:30.60 Carousel.home.jog 0 @10:10:31.10 Carousel.seek 21 @10:10:31.29 Selecting Cartridge 18 @10:10:31.53 Carousel.seek 18 @10:10:32.43 Lysis1.seek :locked @10:10:36.56 Plunge.seek :locked @10:10:36.68 Air.seek :locked @10:10:40.81 Powering cartridge bus @10:10:42.93 Gate.setIR 1750,5000,0,6 @10:10:43.06 Gate.setIR 0,5000,0,6 @10:10:43.11 Loaded Lyse-n-Go Cartridge @10:10:43.13 PuckHeater.configure HeatConfig @10:10:43.18 Gate.power :heat,:ON,limit to 5000mA @10:10:58.61 -> Cmd.startFiltering @10:10:58.84 Duration of filtering limited to 2:05:00 @10:10:59.09 Priming sample loop w/100ml, bypass w/10ml @10:10:59.10 SamplePump.setPosition! 0ml @10:10:59.15 Bypass.open @10:11:00.64 Intake.open @10:11:01.43 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:11:04.27 Vacuum tare is 52% of full scale @10:11:04.39 Pressure.reconfigure :default @10:11:04.45 Pressure.hold @10:11:04.62 SamplePump.reconfigure :prime @10:11:04.69 SamplePump.seek 10ml,1:21 @10:11:13.64 Pressure.coast @10:11:13.69 SamplePump.setPosition! 0ml @10:11:13.74 Exhaust.open @10:11:15.20 Bypass.close @10:11:16.74 Pressure.reconfigure :default @10:11:16.80 Pressure.hold @10:11:17.02 SamplePump.reconfigure :prime @10:11:17.09 SamplePump.seek 100ml,8:51 @10:13:13.40 Pressure.coast @10:13:13.46 Bypass.open @10:13:14.17 Intake.close @10:13:15.01 Toroid.reconfigure :hold @10:13:15.07 Toroid.seek :crack @10:13:15.87 Toroid.reconfigure :default @10:13:15.93 Toroid.seek :clear @10:13:17.49 Toroid.reconfigure :hold @10:13:17.56 Toroid.seek :cartridge @10:13:19.37 Toroid.reconfigure :default @10:13:19.43 Toroid.seek :clear @10:13:21.40 Pressurizing cartridge to 26psi above 13.4psia @10:13:21.41 SamplePump.setPosition! 0ml @10:13:21.46 Intake.open @10:13:22.18 Bypass.close @10:13:22.89 Exhaust.close @10:13:23.72 Pressure.reconfigure :default @10:13:23.77 Pressure.hold @10:13:23.94 SamplePump.reconfigure :charge @10:13:24.01 SamplePump.seek 2.3ml,42 seconds @10:13:33.77 Pressure.coast @10:13:33.82 Intake.close @10:13:34.61 Pressurized cartridge with 0.446ml to 26.0psi @10:13:34.63 Isolated @35.1psia (21.7psi) @10:13:34.64 Checking for cartridge leaks from 35.1psia @10:13:34.67 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @10:13:39.77 Sampling 1000.0ml @10:13:39.79 SamplePump.setPosition! 0ml @10:13:39.84 Exhaust.open @10:13:40.55 Intake.open @10:13:41.40 Pressure.reconfigure :default @10:13:41.45 Pressure.hold @10:13:41.67 SamplePump.reconfigure :sample @10:13:41.74 SamplePump.seek 1000ml,1:23:53 @10:13:44.43 Cartridge 18 sampling 1/1000ml at 0.51ml/s with 26.0psi vacuum @10:14:44.44 Cartridge 18 sampling 33/1000ml at 0.51ml/s with 25.9psi vacuum @10:15:44.44 Cartridge 18 sampling 61/1000ml at 0.43ml/s with 23.5psi vacuum @10:16:44.44 Cartridge 18 sampling 86/1000ml at 0.38ml/s with 22.9psi vacuum @10:17:44.44 Cartridge 18 sampling 108/1000ml at 0.37ml/s with 21.2psi vacuum @10:18:44.82 Cartridge 18 sampling 128/1000ml at 0.30ml/s with 27.4psi vacuum @10:19:44.44 Cartridge 18 sampling 145/1000ml at 0.28ml/s with 23.8psi vacuum @10:20:44.44 Cartridge 18 sampling 160/1000ml at 0.21ml/s with 23.0psi vacuum @10:21:44.44 Cartridge 18 sampling 173/1000ml at 0.20ml/s with 26.2psi vacuum @10:22:44.44 Cartridge 18 sampling 186/1000ml at 0.20ml/s with 29.6psi vacuum @10:23:21.38 Flow averaged 0.34ml/s over 9:33 @10:23:21.41 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @10:23:22.11 Relieved excess vacuum in 7.5 seconds @10:23:22.13 SamplePump.seek 1000ml,1:07:47 @10:23:23.85 Flow estimate reduced to 0.27ml/s @10:23:23.88 Waiting up to 14 seconds for vacuum to reduce from 28.3 to 18.0psi @10:23:24.89 Relieved excess vacuum in 2.6 seconds @10:23:24.91 SamplePump.seek 1000ml,1:07:46 @10:23:44.44 Cartridge 18 sampling 197/1000ml at 0.20ml/s with 26.6psi vacuum @10:23:49.00 Flow averaged 0.25ml/s over 20 seconds @10:23:49.03 Waiting up to 15 seconds for vacuum to reduce from 28.8 to 18.0psi @10:23:49.74 Relieved excess vacuum in 5.2 seconds @10:23:49.76 SamplePump.seek 1000ml,1:07:22 @10:23:51.48 Flow estimate reduced to 0.20ml/s @10:23:51.50 Intake.close @10:23:51.54 Exhaust.close @10:23:51.72 Toroid.reconfigure :hold @10:23:51.80 Toroid.seek :bypass @10:23:53.42 Toroid.reconfigure :default @10:23:53.49 Toroid.seek :clear @10:23:55.11 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @10:23:55.13 Pressure.coast @10:23:55.31 Sampled 198.4ml @10:23:57.22 -> Cmd.startProcessing @10:23:59.07 SPR.command \"read_sequences\" @10:23:59.31 SPR.command \"stats_on\" @10:23:59.35 SPR.command \"start 0 1\" @10:24:01.35 Begun log /var/log/esp/spr19aug2410h.spr @10:24:02.16 Handoff.select :waste @10:24:02.34 Archive.seek :empty @10:24:09.51 Syringe tare is 21% of full scale @10:24:20.06 PinPort.seek :cartridge @10:24:24.62 Lysis1.seek 1350ul @10:24:27.09 Lysis2.seek 1700ul @10:24:30.18 Plunge.seek :reagent @10:24:31.03 Evacuating puck with filter bubble point of 50psi @10:24:31.05 PinPort.seek :cartridge @10:24:31.14 Handoff.select :waste @10:24:41.22 Air pumping 1 strokes to reach 8psi @10:24:41.23 Air.reconfigure :gulp @10:24:41.31 Air.seek :tVent @10:24:43.10 0.4psi after gulp @10:24:43.11 Air stroke #1 of 1 @10:24:43.13 Air.reconfigure :pressurize @10:24:43.20 Air.seek :pressurized @10:24:56.04 0.4psi after pressurize @10:24:56.06 Air.reconfigure :compress @10:24:56.14 Air.seek :evac @10:24:58.80 1.0psi after compress @10:25:12.70 0.1psi after compress (stable) @10:25:13.20 at 0.1psi Opened to waste @10:25:13.21 Handoff.select :blocked @10:25:13.40 Air pumping 10 strokes to reach 50psi @10:25:13.41 Air.reconfigure :gulp @10:25:13.49 Air.seek :tVent @10:25:23.37 7.0psi after gulp @10:25:23.38 Air stroke #1 of 10 @10:25:23.40 Air.reconfigure :pressurize @10:25:23.48 Air.seek :pressurized @10:25:36.34 9.9psi after pressurize @10:25:36.35 Air.reconfigure :compress @10:25:36.43 Air.seek :evac @10:25:39.11 11.2psi after compress @10:26:16.27 31.8psi after compress (stable) @10:26:16.29 Air.reconfigure :gulp @10:26:16.37 Air.seek :tVent @10:26:26.25 33.7psi after gulp @10:26:26.27 Air stroke #2 of 10 @10:26:26.29 Air.reconfigure :pressurize @10:26:26.38 Air.seek :pressurized @10:26:39.22 31.5psi after pressurize @10:26:39.23 Air.reconfigure :compress @10:26:39.31 Air.seek :evac @10:26:41.97 30.6psi after compress @10:27:15.11 45.9psi after compress (stable) @10:27:15.13 Air.reconfigure :gulp @10:27:15.20 Air.seek :tVent @10:27:25.10 47.9psi after gulp @10:27:25.11 Air stroke #3 of 10 @10:27:25.13 Air.reconfigure :pressurize @10:27:25.21 Air.seek :pressurized @10:27:38.04 45.0psi after pressurize @10:27:38.05 Air.reconfigure :compress @10:27:38.13 Air.seek :evac @10:27:40.79 42.3psi after compress @10:27:45.73 Reached 50.1 of 50.0psi after pumping 6230ul (4.00 strokes) @10:27:45.75 Handoff.select :waste @10:28:02.44 delay 30 seconds @10:28:32.46 Handoff.select :blocked @10:28:57.48 Handoff.select :waste @10:28:57.60 PSI at @2.15psi at evacEnd @10:28:57.61 Handoff.select :blocked @10:28:57.74 Air.reconfigure :gulp @10:28:57.81 Air.seek :tVent @10:29:15.29 Stable @4.1psi after Handoff blocked @10:29:15.38 Air pumping 10 strokes to reach 34psi @10:29:15.39 Air stroke #1 of 10 @10:29:15.41 Air.reconfigure :pressurize @10:29:15.49 Air.seek :pressurized @10:29:28.31 4.2psi after pressurize @10:29:28.33 Air.reconfigure :compress @10:29:28.40 Air.seek :evac @10:29:31.07 4.3psi after compress @10:29:58.79 15.2psi after compress (stable) @10:29:58.81 Air.reconfigure :gulp @10:29:58.89 Air.seek :tVent @10:30:08.75 15.7psi after gulp @10:30:08.77 Air stroke #2 of 10 @10:30:08.79 Air.reconfigure :pressurize @10:30:08.87 Air.seek :pressurized @10:30:21.69 15.7psi after pressurize @10:30:21.70 Air.reconfigure :compress @10:30:21.78 Air.seek :evac @10:30:24.44 17.2psi after compress @10:30:48.14 24.6psi after compress (stable) @10:30:48.15 Air.reconfigure :gulp @10:30:48.24 Air.seek :tVent @10:30:58.12 25.2psi after gulp @10:30:58.13 Air stroke #3 of 10 @10:30:58.15 Air.reconfigure :pressurize @10:30:58.23 Air.seek :pressurized @10:31:11.05 25.1psi after pressurize @10:31:11.07 Air.reconfigure :compress @10:31:11.16 Air.seek :evac @10:31:13.82 25.8psi after compress @10:31:36.60 32.7psi after compress (stable) @10:31:36.64 Air.reconfigure :gulp @10:31:36.72 Air.seek :tVent @10:31:46.61 33.4psi after gulp @10:31:46.68 Air stroke #4 of 10 @10:31:46.69 Air.reconfigure :pressurize @10:31:46.77 Air.seek :pressurized @10:31:59.60 33.3psi after pressurize @10:31:59.67 Air.reconfigure :compress @10:31:59.74 Air.seek :evac @10:32:02.41 33.4psi after compress @10:32:02.78 Reached 34.3 of 34.1psi after pumping 7787ul (5.00 strokes) @10:32:02.79 Handoff.select :waste @10:32:22.33 delay 30 seconds @10:32:52.37 Handoff.select :blocked @10:33:17.83 Handoff.select :waste @10:33:17.95 PSI at @0.67psi at evacEnd @10:33:17.96 Air.reconfigure :default @10:33:18.08 Air.seek :fVent @10:33:19.51 delay 5 seconds @10:33:24.53 Seawater evacuated, Presure above/below filter released. Lysis begins @10:33:24.60 Lysis1.reconfigure :slow @10:33:24.66 Lysis1.seek 1050ul @10:33:54.66 delay 5 seconds @10:33:59.68 Handoff.select :blocked @10:33:59.84 Lysis1.seek 450ul @10:34:59.76 delay 3 seconds @10:35:02.86 Air.reconfigure to mix into 1.37806 parts Lysis1 @10:35:03.32 Air.seek :evac @10:35:03.36 Lysis1.seek 300ul @10:35:18.46 Air.reconfigure :default @10:35:18.52 Air.seek :full @10:35:32.73 Lysis1.seek :empty @10:36:02.81 Lysis Delivered. Syringe pressure @20.5psi @10:36:02.85 PuckHeater.seek 84.3C @10:36:51.33 PuckHeater.hold 15:00,84.3C @10:51:49.53 Lysis complete. Syringe pressure @27.7psi @10:51:49.54 Handoff.select :blocked @10:51:49.64 Lysis1.reconfigure :halfSpeed @10:51:49.71 Lysis1.seek 200ul @10:51:53.47 delay 1 second @10:51:54.49 Lysis1.seek :empty @10:51:58.31 Initial AirS pressure @28.0psi @10:51:58.37 Lysis1.reconfigure :extract @10:51:58.46 Lysis1.seek 900ul,10:00 @10:51:59.63 Air pumping 10 strokes to reach 21psi @10:51:59.64 Air stroke #1 of 10 @10:51:59.66 Air.reconfigure :pressurize @10:51:59.75 Air.seek :pressurized @10:52:12.56 15.8psi after pressurize @10:52:12.57 Air.reconfigure :compress @10:52:12.65 Air.seek :evac @10:52:15.32 16.9psi after compress @10:52:29.60 15.8psi after compress (stable) @10:52:29.63 Air.reconfigure :gulp @10:52:29.70 Air.seek :tVent @10:52:39.58 15.6psi after gulp @10:52:39.59 Air stroke #2 of 10 @10:52:39.61 Air.reconfigure :pressurize @10:52:39.69 Air.seek :pressurized @10:52:52.52 15.5psi after pressurize @10:52:52.55 Air.reconfigure :compress @10:52:52.63 Air.seek :evac @10:52:55.30 17.3psi after compress @10:53:09.23 15.3psi after compress (stable) @10:53:09.25 Air.reconfigure :gulp @10:53:09.32 Air.seek :tVent @10:53:19.21 16.1psi after gulp @10:53:19.22 Air stroke #3 of 10 @10:53:19.24 Air.reconfigure :pressurize @10:53:19.32 Air.seek :pressurized @10:53:32.14 15.5psi after pressurize @10:53:32.15 Air.reconfigure :compress @10:53:32.23 Air.seek :evac @10:53:34.92 19.0psi after compress @10:53:35.47 Air.reconfigure :default @10:53:35.52 Air.hold @10:53:35.69 Lysis1 at 900ul @15.5psi @10:53:35.70 Lysis1.reconfigure :default @10:53:35.77 Lysis1.seek 925ul @10:53:36.55 Lysis1 at 925ul, bottom @15.1psi after recovery @10:53:37.66 Lysis1 at 925ul, bottom @17.7psi @10:53:37.68 Air.reconfigure :halfSpeed @10:53:37.75 Air.seek :fVent @10:53:40.00 Puck Top Vented, bottom @3.6psi @10:53:40.01 Handoff.select :waste @10:53:40.12 delay 5 seconds @10:53:45.14 delay 5 seconds @10:53:50.16 Handoff.select SPR @10:53:50.27 SPR.command \"start 2 14\" @10:53:51.77 delay 10 seconds @10:54:01.79 SPR.command \"start 2 0\" @10:54:05.02 Waiting up to 11:00 for SPR to enter state 1 @11:02:37.09 SPR.command \"hold_on\" @11:02:38.49 SPR Dilution 1 of 1 -- Running Experiment 2 @11:02:38.50 Lysis1.reconfigure :DAmix @11:02:38.62 Lysis2.reconfigure :DAmix @11:02:39.01 Lysis1.seek 675ul @11:02:39.04 Lysis2.seek 1450ul @11:03:14.42 Lysis1.seek 375ul @11:03:14.48 Lysis2.seek 1150ul @11:03:56.46 volL1 = 300.0 and volL2 = 300.0 @11:03:56.47 delay 15 seconds @11:04:11.50 SPR.command \"hold_off\" @11:04:14.13 Waiting up to 25:00 for SPR to enter state 7 @11:15:50.64 SPR Analysis has Completed @11:15:50.66 Handoff.select :waste @11:15:51.57 Ended log /var/log/esp/spr19aug2410h.spr @11:15:51.58 SPR.command \"stats_off\" @11:15:52.97 -> Cmd.stop @11:15:53.07 Handoff.select :close @11:15:53.20 Gate.power :heat,:OFF @11:15:53.22 Gate.power :cartridge,:OFF @11:15:53.31 Carousel.seek 18 @11:15:53.48 Plunge.seek :locked @11:15:54.32 PinPort.seek :clear @11:15:58.93 Archive.seek :clear @11:15:59.06 Air.reconfigure :default @11:15:59.19 Lysis1.reconfigure :default @11:15:59.30 Air.seek :clear @11:15:59.36 Lysis1.seek :clear @11:15:59.44 Lysis2.reconfigure :default @11:15:59.52 Lysis2.seek :clear @11:16:16.67 Archive.coast @11:16:16.72 Air.coast @11:16:16.77 Lysis1.coast @11:16:16.82 Lysis2.coast @11:16:16.87 Plunge.coast @11:16:16.92 SamplePump.coast @11:16:16.97 Toroid.coast @11:16:17.02 Carousel.coast @11:16:17.07 PinPort.coast @11:16:17.25 Gate.power :motor,:OFF @11:16:17.27 Gate.power :core,:OFF @11:16:17.29 Gate.power :analytic1,:OFF @11:16:17.31 Gate.power :analytic2,:OFF @11:16:17.33 Gate.power :analytic3,:OFF @11:16:17.35 Gate.power :main,:OFF @11:16:17.37 Safely stopped and ready to power off ##-- file closed 2019-08-24T20:52:38.861Z ##-- file opened 2019-08-24T20:52:38.863Z @16:52:39.56 -> showStatus @16:52:41.58 -> Cmd.loadCartridge -4 @16:52:41.72 Gate.power :main,:ON,limit to 5000mA @16:52:41.73 Gate.power :analytic1,:ON,limit to 5000mA @16:52:41.75 Gate.power :analytic2,:ON,limit to 5000mA @16:52:41.78 Gate.power :analytic3,:ON,limit to 5000mA @16:52:41.91 Powering core bus @16:52:44.19 SPR Module 1, FW Rev. B, FPGA Rev. C @16:52:44.32 Archive.configure :default @16:52:44.44 Air.configure :default @16:52:44.54 Lysis1.configure :default @16:52:44.64 Lysis2.configure :default @16:52:44.76 Plunge.configure :default @16:52:44.88 SamplePump.configure :default @16:52:45.00 ExtraPump.configure :default @16:52:45.11 Toroid.configure :default @16:52:45.24 Carousel.configure :default @16:52:45.36 Pressure.configure :default @16:52:45.47 PinPort.configure :default @16:52:45.52 All elf controllers running firmware version 4.58 @16:52:45.55 Gate.power :motor,:ON,limit to 5000mA @16:52:45.73 Archive.reconfigure :default @16:52:46.03 Archive.home.jog 0 @16:52:46.16 Toroid.reconfigure :default @16:52:46.25 Plunge.reconfigure :default @16:52:46.28 Lysis1.reconfigure :default @16:52:46.32 PinPort.reconfigure :default @16:52:46.35 Lysis2.reconfigure :default @16:52:46.44 Air.reconfigure :default @16:52:46.66 Air.home.jog 0 @16:52:46.71 PinPort.home.jog 0 @16:52:46.76 Plunge.home.jog 0 @16:52:46.82 Toroid.home.jog 0 @16:52:46.88 Lysis2.home.jog 0 @16:52:46.94 Lysis1.home.jog 0 @16:52:47.22 Archive.reconfigure :homing @16:52:47.46 Toroid.reconfigure :homing @16:52:47.52 Plunge.reconfigure :homing @16:52:47.55 PinPort.reconfigure :homing @16:52:47.59 Lysis1.reconfigure :homing @16:52:47.63 Lysis2.reconfigure :homing @16:52:47.67 Air.reconfigure :homing @16:52:47.89 Lysis1.jog 1400 @16:52:47.94 Air.jog 1409 @16:52:47.99 Toroid.jog 623 @16:52:48.04 Plunge.jog 1542 @16:52:48.10 PinPort.jog -106 @16:52:48.16 Archive.jog 1383 @16:52:48.22 Lysis2.jog 1428 @16:52:48.33 PinPort.setRawPosition! 0 @16:52:48.37 PinPort.seek :home @16:52:48.46 PinPort.reconfigure :default @16:52:49.04 Lysis1.setRawPosition! 50 @16:52:49.09 Lysis1.seek :home @16:52:49.19 Air.setRawPosition! 50 @16:52:49.23 Air.seek :home @16:52:49.34 Archive.setRawPosition! 50 @16:52:49.38 Plunge.setRawPosition! 50 @16:52:49.44 Archive.seek :home @16:52:49.48 Plunge.seek :home @16:52:49.63 Lysis2.setRawPosition! 50 @16:52:49.66 Air.reconfigure :default @16:52:49.69 Lysis1.reconfigure :default @16:52:49.74 Lysis2.seek :home @16:52:49.94 Plunge.reconfigure :default @16:52:49.96 Archive.reconfigure :default @16:52:50.13 Toroid.setRawPosition! 0 @16:52:50.18 Toroid.seek :home @16:52:50.27 Toroid.reconfigure :default @16:52:50.30 Lysis2.reconfigure :default @16:52:51.07 Toroid.seek :clear @16:52:52.69 Carousel.reconfigure :default @16:52:52.76 Carousel.home.jog 0 @16:52:53.25 Carousel.seek 18 @16:52:53.42 Selecting Cartridge 19 @16:52:53.66 Carousel.seek 19 @16:52:54.15 Lysis1.seek :locked @16:52:58.28 Plunge.seek :locked @16:52:58.40 Air.seek :locked @16:53:02.53 Powering cartridge bus @16:53:04.65 Gate.setIR 1750,5000,0,6 @16:53:04.78 Gate.setIR 0,5000,0,6 @16:53:04.82 Loaded Archive Cartridge Gate.power :cartridge,:OFF @16:53:20.51 -> Cmd.startFiltering @16:53:20.74 Duration of filtering limited to 2:05:00 @16:53:20.99 Priming sample loop w/100ml, bypass w/10ml @16:53:21.00 SamplePump.setPosition! 0ml @16:53:21.05 Bypass.open @16:53:22.54 Intake.open @16:53:23.39 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:53:26.23 Vacuum tare is 52% of full scale @16:53:26.36 Pressure.reconfigure :default @16:53:26.42 Pressure.hold @16:53:26.58 SamplePump.reconfigure :prime @16:53:26.66 SamplePump.seek 10ml,1:21 @16:53:35.60 Pressure.coast @16:53:35.65 SamplePump.setPosition! 0ml @16:53:35.70 Exhaust.open @16:53:37.10 Bypass.close @16:53:38.67 Pressure.reconfigure :default @16:53:38.73 Pressure.hold @16:53:38.96 SamplePump.reconfigure :prime @16:53:39.03 SamplePump.seek 100ml,8:51 @16:55:35.41 Pressure.coast @16:55:35.47 Bypass.open @16:55:36.22 Intake.close @16:55:37.07 Toroid.reconfigure :hold @16:55:37.13 Toroid.seek :crack @16:55:37.93 Toroid.reconfigure :default @16:55:37.99 Toroid.seek :clear @16:55:39.55 Toroid.reconfigure :hold @16:55:39.62 Toroid.seek :cartridge @16:55:41.43 Toroid.reconfigure :default @16:55:41.49 Toroid.seek :clear @16:55:43.46 Pressurizing cartridge to 26psi above 14.7psia @16:55:43.47 SamplePump.setPosition! 0ml @16:55:43.51 Intake.open @16:55:44.24 Bypass.close @16:55:44.96 Exhaust.close @16:55:45.74 Pressure.reconfigure :default @16:55:45.79 Pressure.hold @16:55:45.96 SamplePump.reconfigure :charge @16:55:46.04 SamplePump.seek 2.3ml,42 seconds @16:55:54.18 Pressure.coast @16:55:54.23 Intake.close @16:55:55.03 Pressurized cartridge with 0.310ml to 26.3psi @16:55:55.04 Isolated @36.6psia (21.9psi) @16:55:55.05 Checking for cartridge leaks from 36.6psia @16:55:55.08 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @16:56:00.16 Cartridge pressure fell 1% to 36.3psia @16:56:00.24 Sampling 1000.0ml @16:56:00.25 SamplePump.setPosition! 0ml @16:56:00.30 Exhaust.open @16:56:00.99 Intake.open @16:56:01.83 Pressure.reconfigure :default @16:56:01.89 Pressure.hold @16:56:02.10 SamplePump.reconfigure :sample @16:56:02.17 SamplePump.seek 1000ml,1:23:53 @16:56:04.87 Cartridge 19 sampling 1/1000ml at 0.41ml/s with 21.1psi vacuum @16:57:04.87 Cartridge 19 sampling 25/1000ml at 0.41ml/s with 24.2psi vacuum @16:58:04.87 Cartridge 19 sampling 47/1000ml at 0.38ml/s with 22.5psi vacuum @16:59:04.87 Cartridge 19 sampling 68/1000ml at 0.35ml/s with 21.5psi vacuum @17:00:04.87 Cartridge 19 sampling 88/1000ml at 0.30ml/s with 22.4psi vacuum @17:01:05.25 Cartridge 19 sampling 105/1000ml at 0.27ml/s with 27.1psi vacuum @17:02:04.87 Cartridge 19 sampling 120/1000ml at 0.22ml/s with 26.3psi vacuum @17:03:04.87 Cartridge 19 sampling 132/1000ml at 0.20ml/s with 23.7psi vacuum @17:03:36.35 Flow averaged 0.31ml/s over 7:33 @17:03:36.38 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi @17:03:37.38 Relieved excess vacuum in 2.4 seconds @17:03:37.40 SamplePump.seek 1000ml,1:12:21 @17:04:03.09 Flow averaged 0.22ml/s over 24 seconds @17:04:03.12 Waiting up to 13 seconds for vacuum to reduce from 27.8 to 18.0psi @17:04:04.12 Relieved excess vacuum in 3.6 seconds @17:04:04.14 SamplePump.seek 1000ml,1:11:55 @17:04:04.86 Cartridge 19 sampling 144/1000ml at 0.28ml/s with 14.0psi vacuum @17:05:04.87 Cartridge 19 sampling 156/1000ml at 0.20ml/s with 23.6psi vacuum @17:06:04.87 Cartridge 19 sampling 168/1000ml at 0.21ml/s with 24.4psi vacuum @17:07:04.87 Cartridge 19 sampling 180/1000ml at 0.20ml/s with 25.2psi vacuum @17:07:20.15 Flow averaged 0.21ml/s over 3:11 @17:07:20.18 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @17:07:21.17 Relieved excess vacuum in 6.2 seconds @17:07:21.20 SamplePump.seek 1000ml,1:08:37 @17:07:35.91 Flow averaged 0.21ml/s over 15 seconds @17:07:35.94 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi @17:07:36.94 Relieved excess vacuum in 1.6 seconds @17:07:36.96 SamplePump.seek 1000ml,1:08:22 @17:07:45.06 Flow estimate reduced to 0.17ml/s @17:07:45.09 Intake.close @17:07:45.14 Exhaust.close @17:07:45.31 Toroid.reconfigure :hold @17:07:45.38 Toroid.seek :bypass @17:07:47.00 Toroid.reconfigure :default @17:07:47.07 Toroid.seek :clear @17:07:48.69 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @17:07:48.71 Pressure.coast @17:07:48.89 Sampled 187.6ml @17:07:50.99 -> Cmd.startProcessing @17:07:51.24 Plunge.seek :reagent @17:07:52.09 Sample loop closed. Ready to Deliver Reagents @17:07:52.10 Archive.seek :sealed @17:07:56.39 Archive.seek :evac @17:08:10.33 delay 1:00 @17:09:10.36 Archive.seek :clear @17:09:28.07 RNAlater soak for 10:00 @17:19:28.09 Archive.seek :tVent @17:19:30.67 delay 1 second @17:19:31.69 Archive.seek :evac @17:19:47.32 delay 10 seconds @17:19:57.35 Archive.seek :tVent @17:20:12.97 delay 1 second @17:20:13.99 Archive.seek :evac @17:20:29.61 delay 10 seconds @17:20:39.64 Sample preserved and evacuated @17:20:40.56 -> Cmd.stop @17:20:40.70 Carousel.seek 19 @17:20:40.87 Plunge.seek :locked @17:20:41.71 PinPort.seek :clear @17:20:41.85 Archive.seek :clear @17:20:41.98 Air.seek :clear @17:20:42.11 Lysis1.seek :clear @17:20:59.59 Archive.coast @17:20:59.65 Air.coast @17:20:59.69 Lysis1.coast @17:20:59.74 Lysis2.coast @17:20:59.79 Plunge.coast @17:20:59.84 SamplePump.coast @17:20:59.89 Toroid.coast @17:20:59.94 Carousel.coast @17:20:59.99 PinPort.coast @17:21:00.17 Gate.power :motor,:OFF @17:21:00.19 Gate.power :core,:OFF @17:21:00.21 Gate.power :analytic1,:OFF @17:21:00.23 Gate.power :analytic2,:OFF @17:21:00.25 Gate.power :analytic3,:OFF @17:21:00.27 Gate.power :main,:OFF @17:21:00.29 Safely stopped and ready to power off ##-- file closed 2019-08-24T21:36:49.249Z ##-- file opened 2019-08-24T21:36:49.251Z @17:36:49.79 -> showStatus @17:36:51.88 -> Cmd.loadCartridge -15 @17:36:52.02 Gate.power :main,:ON,limit to 5000mA @17:36:52.03 Gate.power :analytic1,:ON,limit to 5000mA @17:36:52.05 Gate.power :analytic2,:ON,limit to 5000mA @17:36:52.07 Gate.power :analytic3,:ON,limit to 5000mA @17:36:52.21 Powering core bus @17:36:54.48 SPR Module 1, FW Rev. B, FPGA Rev. C @17:36:54.61 Archive.configure :default @17:36:54.73 Air.configure :default @17:36:54.84 Lysis1.configure :default @17:36:54.95 Lysis2.configure :default @17:36:55.07 Plunge.configure :default @17:36:55.19 SamplePump.configure :default @17:36:55.31 ExtraPump.configure :default @17:36:55.43 Toroid.configure :default @17:36:55.55 Carousel.configure :default @17:36:55.67 Pressure.configure :default @17:36:55.77 PinPort.configure :default @17:36:55.86 All elf controllers running firmware version 4.58 @17:36:55.89 Gate.power :motor,:ON,limit to 5000mA @17:36:56.14 Archive.reconfigure :default @17:36:56.24 Air.reconfigure :default @17:36:56.45 Archive.home.jog 0 @17:36:56.50 Air.home.jog 0 @17:36:56.66 PinPort.reconfigure :default @17:36:56.70 Toroid.reconfigure :default @17:36:56.77 Lysis2.reconfigure :default @17:36:56.80 Lysis1.reconfigure :default @17:36:56.87 Plunge.reconfigure :default @17:36:57.09 PinPort.home.jog 0 @17:36:57.15 Lysis2.home.jog 0 @17:36:57.20 Lysis1.home.jog 0 @17:36:57.27 Toroid.home.jog 0 @17:36:57.33 Plunge.home.jog 0 @17:36:57.57 Archive.reconfigure :homing @17:36:57.61 Air.reconfigure :homing @17:36:57.78 Archive.jog 1382 @17:36:57.83 Air.jog 1404 @17:36:57.99 Toroid.reconfigure :homing @17:36:58.02 Plunge.reconfigure :homing @17:36:58.06 PinPort.reconfigure :homing @17:36:58.10 Lysis1.reconfigure :homing @17:36:58.16 Lysis2.reconfigure :homing @17:36:58.32 Plunge.jog 1543 @17:36:58.38 Lysis1.jog 1400 @17:36:58.44 Toroid.jog 627 @17:36:58.50 PinPort.jog -108 @17:36:58.56 Lysis2.jog 1427 @17:36:58.73 PinPort.setRawPosition! 0 @17:36:58.77 PinPort.seek :home @17:36:58.86 PinPort.reconfigure :default @17:36:58.94 Archive.setRawPosition! 50 @17:36:59.00 Archive.seek :home @17:36:59.08 Air.setRawPosition! 50 @17:36:59.12 Air.seek :home @17:36:59.28 Archive.reconfigure :default @17:36:59.41 Air.reconfigure :default @17:36:59.55 Plunge.setRawPosition! 50 @17:36:59.60 Plunge.seek :home @17:36:59.69 Lysis1.setRawPosition! 50 @17:36:59.74 Lysis1.seek :home @17:36:59.83 Lysis2.setRawPosition! 50 @17:36:59.90 Lysis2.seek :home @17:36:59.99 Plunge.reconfigure :default @17:37:00.06 Lysis1.reconfigure :default @17:37:00.18 Lysis2.reconfigure :default @17:37:00.57 Toroid.setRawPosition! 0 @17:37:00.62 Toroid.seek :home @17:37:01.06 Toroid.reconfigure :default @17:37:01.18 Toroid.seek :clear @17:37:02.81 Carousel.reconfigure :default @17:37:02.89 Carousel.home.jog 0 @17:37:03.38 Carousel.seek 19 @17:37:03.54 Selecting Cartridge 16 @17:37:03.78 Carousel.seek 16 @17:37:04.67 Lysis1.seek :locked @17:37:08.80 Plunge.seek :locked @17:37:08.92 Air.seek :locked @17:37:13.05 Powering cartridge bus @17:37:15.17 Gate.setIR 1750,5000,0,6 @17:37:15.30 Gate.setIR 0,5000,0,6 @17:37:15.35 Loaded Lyse-n-Go Cartridge @17:37:15.37 PuckHeater.configure HeatConfig @17:37:15.42 Gate.power :heat,:ON,limit to 5000mA @17:37:31.13 -> Cmd.startFiltering @17:37:31.35 Duration of filtering limited to 2:05:00 @17:37:31.61 Priming sample loop w/100ml, bypass w/10ml @17:37:31.62 SamplePump.setPosition! 0ml @17:37:31.68 Bypass.open @17:37:33.14 Intake.open @17:37:33.95 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:37:36.78 Vacuum tare is 52% of full scale @17:37:36.91 Pressure.reconfigure :default @17:37:36.96 Pressure.hold @17:37:37.14 SamplePump.reconfigure :prime @17:37:37.22 SamplePump.seek 10ml,1:21 @17:37:46.16 Pressure.coast @17:37:46.21 SamplePump.setPosition! 0ml @17:37:46.26 Exhaust.open @17:37:47.70 Bypass.close @17:37:49.23 Pressure.reconfigure :default @17:37:49.29 Pressure.hold @17:37:49.51 SamplePump.reconfigure :prime @17:37:49.59 SamplePump.seek 100ml,8:51 @17:39:45.86 Pressure.coast @17:39:45.92 Bypass.open @17:39:46.65 Intake.close @17:39:47.47 Toroid.reconfigure :hold @17:39:47.53 Toroid.seek :crack @17:39:48.33 Toroid.reconfigure :default @17:39:48.39 Toroid.seek :clear @17:39:49.95 Toroid.reconfigure :hold @17:39:50.02 Toroid.seek :cartridge @17:39:51.83 Toroid.reconfigure :default @17:39:51.89 Toroid.seek :clear @17:39:53.85 Pressurizing cartridge to 26psi above 12.8psia @17:39:53.87 SamplePump.setPosition! 0ml @17:39:53.91 Intake.open @17:39:54.68 Bypass.close @17:39:55.44 Exhaust.close @17:39:56.23 Pressure.reconfigure :default @17:39:56.29 Pressure.hold @17:39:56.45 SamplePump.reconfigure :charge @17:39:56.53 SamplePump.seek 2.3ml,42 seconds @17:40:07.84 Pressure.coast @17:40:07.89 Intake.close @17:40:08.72 Pressurized cartridge with 0.601ml to 26.7psi @17:40:08.73 Isolated @35.4psia (22.6psi) @17:40:08.75 Checking for cartridge leaks from 35.4psia @17:40:08.78 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @17:40:13.88 Sampling 1000.0ml @17:40:13.89 SamplePump.setPosition! 0ml @17:40:13.94 Exhaust.open @17:40:14.63 Intake.open @17:40:15.47 Pressure.reconfigure :default @17:40:15.53 Pressure.hold @17:40:15.74 SamplePump.reconfigure :sample @17:40:15.82 SamplePump.seek 1000ml,1:23:53 @17:40:18.51 Cartridge 16 sampling 1/1000ml at 0.53ml/s with 24.9psi vacuum @17:41:18.50 Cartridge 16 sampling 33/1000ml at 0.52ml/s with 25.0psi vacuum @17:42:18.49 Cartridge 16 sampling 64/1000ml at 0.49ml/s with 23.9psi vacuum @17:43:18.50 Cartridge 16 sampling 94/1000ml at 0.48ml/s with 23.8psi vacuum @17:44:18.88 Cartridge 16 sampling 122/1000ml at 0.45ml/s with 26.7psi vacuum @17:45:18.50 Cartridge 16 sampling 148/1000ml at 0.44ml/s with 22.0psi vacuum @17:46:18.50 Cartridge 16 sampling 173/1000ml at 0.42ml/s with 24.3psi vacuum @17:47:18.50 Cartridge 16 sampling 196/1000ml at 0.36ml/s with 22.3psi vacuum @17:48:18.50 Cartridge 16 sampling 218/1000ml at 0.33ml/s with 21.9psi vacuum @17:49:18.50 Cartridge 16 sampling 238/1000ml at 0.36ml/s with 25.5psi vacuum @17:50:18.50 Cartridge 16 sampling 257/1000ml at 0.31ml/s with 26.6psi vacuum @17:51:18.50 Cartridge 16 sampling 274/1000ml at 0.30ml/s with 22.3psi vacuum @17:52:18.50 Cartridge 16 sampling 290/1000ml at 0.27ml/s with 24.0psi vacuum @17:53:18.50 Cartridge 16 sampling 305/1000ml at 0.24ml/s with 23.2psi vacuum @17:54:18.50 Cartridge 16 sampling 318/1000ml at 0.21ml/s with 24.8psi vacuum @17:55:18.50 Cartridge 16 sampling 331/1000ml at 0.21ml/s with 22.9psi vacuum @17:56:18.50 Cartridge 16 sampling 343/1000ml at 0.20ml/s with 27.6psi vacuum @17:57:18.50 Cartridge 16 sampling 356/1000ml at 0.20ml/s with 24.0psi vacuum @17:58:18.50 Cartridge 16 sampling 368/1000ml at 0.20ml/s with 27.2psi vacuum @17:58:26.94 Flow averaged 0.34ml/s over 18:03 @17:58:26.97 Waiting up to 14 seconds for vacuum to reduce from 28.5 to 18.0psi @17:58:27.97 Relieved excess vacuum in 9.4 seconds @17:58:27.99 SamplePump.seek 1000ml,53:06 @17:58:54.14 Flow averaged 0.23ml/s over 24 seconds @17:58:54.17 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @17:58:54.87 Relieved excess vacuum in 3.8 seconds @17:58:54.89 SamplePump.seek 1000ml,52:39 @17:59:04.09 Flow estimate reduced to 0.18ml/s @17:59:04.11 Intake.close @17:59:04.16 Exhaust.close @17:59:04.33 Toroid.reconfigure :hold @17:59:04.40 Toroid.seek :bypass @17:59:06.02 Toroid.reconfigure :default @17:59:06.10 Toroid.seek :clear @17:59:07.72 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @17:59:07.74 Pressure.coast @17:59:07.92 Sampled 376.3ml @17:59:10.14 -> Cmd.startProcessing @17:59:11.99 SPR.command \"read_sequences\" @17:59:12.73 SPR.command \"stats_on\" @17:59:12.77 SPR.command \"start 0 1\" @17:59:14.77 Begun log /var/log/esp/spr19aug2417h.spr @17:59:15.58 Handoff.select :waste @17:59:15.76 Archive.seek :empty @17:59:22.97 Syringe tare is 20% of full scale @17:59:33.47 PinPort.seek :cartridge @17:59:38.03 Lysis1.seek 1350ul @17:59:40.50 Lysis2.seek 1700ul @17:59:43.59 Plunge.seek :reagent @17:59:44.43 Evacuating puck with filter bubble point of 50psi @17:59:44.46 PinPort.seek :cartridge @17:59:44.54 Handoff.select :waste @17:59:51.87 Air pumping 1 strokes to reach 8psi @17:59:51.89 Air.reconfigure :gulp @17:59:51.97 Air.seek :tVent @17:59:53.79 -0.0psi after gulp @17:59:53.80 Air stroke #1 of 1 @17:59:53.82 Air.reconfigure :pressurize @17:59:53.91 Air.seek :pressurized @18:00:06.74 -0.1psi after pressurize @18:00:06.75 Air.reconfigure :compress @18:00:06.83 Air.seek :evac @18:00:09.49 0.4psi after compress @18:00:23.79 0.2psi after compress (stable) @18:00:23.91 at 0.3psi Opened to waste @18:00:23.92 Handoff.select :blocked @18:00:24.10 Air pumping 10 strokes to reach 50psi @18:00:24.12 Air.reconfigure :gulp @18:00:24.21 Air.seek :tVent @18:00:34.10 9.1psi after gulp @18:00:34.11 Air stroke #1 of 10 @18:00:34.13 Air.reconfigure :pressurize @18:00:34.21 Air.seek :pressurized @18:00:47.03 11.9psi after pressurize @18:00:47.04 Air.reconfigure :compress @18:00:47.12 Air.seek :evac @18:00:49.78 13.6psi after compress @18:01:24.29 34.8psi after compress (stable) @18:01:24.31 Air.reconfigure :gulp @18:01:24.38 Air.seek :tVent @18:01:34.28 36.3psi after gulp @18:01:34.29 Air stroke #2 of 10 @18:01:34.31 Air.reconfigure :pressurize @18:01:34.39 Air.seek :pressurized @18:01:47.23 34.1psi after pressurize @18:01:47.24 Air.reconfigure :compress @18:01:47.32 Air.seek :evac @18:01:50.00 33.1psi after compress @18:02:16.61 Reached 50.0 of 50.0psi after pumping 4672ul (3.00 strokes) @18:02:16.62 Handoff.select :waste @18:02:34.97 delay 30 seconds @18:03:05.00 Handoff.select :blocked @18:03:22.30 Pressure too High, open to waste again (8.1psi) @18:03:22.32 Handoff.select :waste @18:03:22.44 delay 30 seconds @18:03:52.46 Handoff.select :blocked @18:04:18.54 Handoff.select :waste @18:04:19.06 PSI at @5.20psi at evacEnd @18:04:19.07 Handoff.select :blocked @18:04:19.20 Air.reconfigure :gulp @18:04:19.32 Air.seek :tVent @18:04:36.38 Stable @3.9psi after Handoff blocked @18:04:36.47 Air pumping 10 strokes to reach 34psi @18:04:36.48 Air stroke #1 of 10 @18:04:36.50 Air.reconfigure :pressurize @18:04:36.58 Air.seek :pressurized @18:04:49.41 4.3psi after pressurize @18:04:49.43 Air.reconfigure :compress @18:04:49.50 Air.seek :evac @18:04:52.17 5.3psi after compress @18:05:12.17 10.6psi after compress (stable) @18:05:12.19 Air.reconfigure :gulp @18:05:12.27 Air.seek :tVent @18:05:22.18 14.9psi after gulp @18:05:22.19 Air stroke #2 of 10 @18:05:22.21 Air.reconfigure :pressurize @18:05:22.33 Air.seek :pressurized @18:05:35.19 15.3psi after pressurize @18:05:35.20 Air.reconfigure :compress @18:05:35.29 Air.seek :evac @18:05:37.96 16.3psi after compress @18:06:01.25 25.2psi after compress (stable) @18:06:01.27 Air.reconfigure :gulp @18:06:01.35 Air.seek :tVent @18:06:11.23 25.8psi after gulp @18:06:11.60 Air stroke #3 of 10 @18:06:11.65 Air.reconfigure :pressurize @18:06:11.73 Air.seek :pressurized @18:06:24.58 25.8psi after pressurize @18:06:24.60 Air.reconfigure :compress @18:06:24.69 Air.seek :evac @18:06:27.40 26.1psi after compress @18:06:44.69 Reached 33.9 of 33.9psi after pumping 6230ul (4.00 strokes) @18:06:44.70 Handoff.select :waste @18:06:56.47 delay 30 seconds @18:07:26.49 Handoff.select :blocked @18:07:51.52 Handoff.select :waste @18:07:51.66 PSI at @2.24psi at evacEnd @18:07:51.67 Air.reconfigure :default @18:07:51.79 Air.seek :fVent @18:07:53.22 delay 5 seconds @18:07:58.24 Seawater evacuated, Presure above/below filter released. Lysis begins @18:07:58.31 Lysis1.reconfigure :slow @18:07:58.37 Lysis1.seek 1050ul @18:08:28.39 delay 5 seconds @18:08:33.42 Handoff.select :blocked @18:08:33.58 Lysis1.seek 450ul @18:09:33.56 delay 3 seconds @18:09:36.64 Air.reconfigure to mix into 1.37806 parts Lysis1 @18:09:37.02 Air.seek :evac @18:09:37.06 Lysis1.seek 300ul @18:09:52.16 Air.reconfigure :default @18:09:52.22 Air.seek :full @18:10:06.43 Lysis1.seek :empty @18:10:36.51 Lysis Delivered. Syringe pressure @30.7psi @18:10:36.55 PuckHeater.seek 84.2C @18:11:32.39 PuckHeater.hold 15:00,84.2C @18:26:28.86 Lysis complete. Syringe pressure @38.8psi @18:26:28.88 Handoff.select :blocked @18:26:28.98 Lysis1.reconfigure :halfSpeed @18:26:29.04 Lysis1.seek 200ul @18:26:32.81 delay 1 second @18:26:33.83 Lysis1.seek :empty @18:26:37.66 Initial AirS pressure @39.8psi @18:26:37.72 Lysis1.reconfigure :extract @18:26:37.81 Lysis1.seek 900ul,10:00 @18:26:39.17 Air pumping 10 strokes to reach 21psi @18:26:39.18 Air stroke #1 of 10 @18:26:39.21 Air.reconfigure :pressurize @18:26:39.29 Air.seek :pressurized @18:26:52.12 15.7psi after pressurize @18:26:52.13 Air.reconfigure :compress @18:26:52.22 Air.seek :evac @18:26:54.88 17.7psi after compress @18:27:09.13 15.7psi after compress (stable) @18:27:09.15 Air.reconfigure :gulp @18:27:09.24 Air.seek :tVent @18:27:19.13 16.2psi after gulp @18:27:19.14 Air stroke #2 of 10 @18:27:19.16 Air.reconfigure :pressurize @18:27:19.24 Air.seek :pressurized @18:27:32.08 16.0psi after pressurize @18:27:32.09 Air.reconfigure :compress @18:27:32.17 Air.seek :evac @18:27:34.85 15.2psi after compress @18:27:48.78 15.7psi after compress (stable) @18:27:48.80 Air.reconfigure :gulp @18:27:48.87 Air.seek :tVent @18:27:58.77 16.0psi after gulp @18:27:58.78 Air stroke #3 of 10 @18:27:58.80 Air.reconfigure :pressurize @18:27:58.88 Air.seek :pressurized @18:28:11.73 15.3psi after pressurize @18:28:11.74 Air.reconfigure :compress @18:28:11.82 Air.seek :evac @18:28:14.50 15.7psi after compress @18:28:25.19 Air.reconfigure :default @18:28:25.25 Air.hold @18:28:25.41 Lysis1 at 900ul @16.4psi @18:28:25.44 Lysis1.reconfigure :default @18:28:25.51 Lysis1.seek 925ul @18:28:26.28 Lysis1 at 925ul, bottom @11.9psi after recovery @18:28:27.39 Lysis1 at 925ul, bottom @15.0psi @18:28:27.41 Air.reconfigure :halfSpeed @18:28:27.48 Air.seek :fVent @18:28:29.73 Puck Top Vented, bottom @22.6psi @18:28:29.74 Handoff.select :waste @18:28:29.86 delay 5 seconds @18:28:34.88 delay 5 seconds @18:28:39.90 Handoff.select SPR @18:28:40.01 SPR.command \"start 2 14\" @18:28:41.55 delay 10 seconds @18:28:51.57 SPR.command \"start 2 0\" @18:28:54.80 Waiting up to 11:00 for SPR to enter state 1 @18:37:26.87 SPR.command \"hold_on\" @18:37:28.27 SPR Dilution 1 of 1 -- Running Experiment 2 @18:37:28.28 Lysis2.reconfigure :DAmix @18:37:28.40 Lysis1.reconfigure :DAmix @18:37:28.82 Lysis2.seek 1450ul @18:37:28.86 Lysis1.seek 675ul @18:38:04.28 Lysis2.seek 1150ul @18:38:04.33 Lysis1.seek 375ul @18:38:46.34 volL1 = 300.0 and volL2 = 300.0 @18:38:46.35 delay 15 seconds @18:39:01.38 SPR.command \"hold_off\" @18:39:03.91 Waiting up to 25:00 for SPR to enter state 7 @18:50:40.44 SPR Analysis has Completed @18:50:40.45 Handoff.select :waste @18:50:41.35 Ended log /var/log/esp/spr19aug2417h.spr @18:50:41.36 SPR.command \"stats_off\" @18:50:42.64 -> Cmd.stop @18:50:42.73 Handoff.select :close @18:50:42.86 Gate.power :heat,:OFF @18:50:42.88 Gate.power :cartridge,:OFF @18:50:42.96 Carousel.seek 16 @18:50:43.13 Plunge.seek :locked @18:50:43.96 PinPort.seek :clear @18:50:48.57 Archive.seek :clear @18:50:48.70 Air.reconfigure :default @18:50:48.83 Lysis1.reconfigure :default @18:50:48.94 Air.seek :clear @18:50:49.00 Lysis1.seek :clear @18:50:49.08 Lysis2.reconfigure :default @18:50:49.15 Lysis2.seek :clear @18:51:06.32 Archive.coast @18:51:06.37 Air.coast @18:51:06.42 Lysis1.coast @18:51:06.47 Lysis2.coast @18:51:06.52 Plunge.coast @18:51:06.57 SamplePump.coast @18:51:06.62 Toroid.coast @18:51:06.67 Carousel.coast @18:51:06.72 PinPort.coast @18:51:06.91 Gate.power :motor,:OFF @18:51:06.92 Gate.power :core,:OFF @18:51:06.95 Gate.power :analytic1,:OFF @18:51:06.97 Gate.power :analytic2,:OFF @18:51:06.99 Gate.power :analytic3,:OFF @18:51:07.01 Gate.power :main,:OFF @18:51:07.03 Safely stopped and ready to power off ##-- file closed 2019-08-25T11:27:48.760Z ##-- file opened 2019-08-25T11:27:48.765Z @07:27:48.60 -> showStatus @07:27:50.62 -> Cmd.loadCartridge -4 @07:27:50.76 Gate.power :main,:ON,limit to 5000mA @07:27:50.77 Gate.power :analytic1,:ON,limit to 5000mA @07:27:50.79 Gate.power :analytic2,:ON,limit to 5000mA @07:27:50.82 Gate.power :analytic3,:ON,limit to 5000mA @07:27:50.95 Powering core bus @07:27:53.26 SPR Module 1, FW Rev. B, FPGA Rev. C @07:27:53.38 Archive.configure :default @07:27:53.50 Air.configure :default @07:27:53.60 Lysis1.configure :default @07:27:53.70 Lysis2.configure :default @07:27:53.82 Plunge.configure :default @07:27:53.94 SamplePump.configure :default @07:27:54.06 ExtraPump.configure :default @07:27:54.18 Toroid.configure :default @07:27:54.30 Carousel.configure :default @07:27:54.42 Pressure.configure :default @07:27:54.52 PinPort.configure :default @07:27:54.58 All elf controllers running firmware version 4.58 @07:27:54.60 Gate.power :motor,:ON,limit to 5000mA @07:27:54.77 Archive.reconfigure :default @07:27:55.07 Archive.home.jog 0 @07:27:55.22 PinPort.reconfigure :default @07:27:55.27 Toroid.reconfigure :default @07:27:55.32 Lysis2.reconfigure :default @07:27:55.36 Air.reconfigure :default @07:27:55.40 Lysis1.reconfigure :default @07:27:55.50 Plunge.reconfigure :default @07:27:55.69 Lysis2.home.jog 0 @07:27:55.74 Air.home.jog 0 @07:27:55.79 Toroid.home.jog 0 @07:27:55.85 Lysis1.home.jog 0 @07:27:55.90 PinPort.home.jog 0 @07:27:55.96 Plunge.home.jog 0 @07:27:56.28 Archive.reconfigure :homing @07:27:56.36 Archive.jog 1378 @07:27:56.53 Plunge.reconfigure :homing @07:27:56.56 Air.reconfigure :homing @07:27:56.59 Toroid.reconfigure :homing @07:27:56.64 Lysis2.reconfigure :homing @07:27:56.68 Lysis1.reconfigure :homing @07:27:56.71 PinPort.reconfigure :homing @07:27:56.92 Lysis1.jog 1398 @07:27:56.98 Toroid.jog 626 @07:27:57.04 Air.jog 1406 @07:27:57.10 Plunge.jog 1541 @07:27:57.16 Lysis2.jog 1427 @07:27:57.21 PinPort.jog -106 @07:27:57.44 PinPort.setRawPosition! 0 @07:27:57.49 PinPort.seek :home @07:27:57.59 Archive.setRawPosition! 50 @07:27:57.61 PinPort.reconfigure :default @07:27:57.68 Archive.seek :home @07:27:57.98 Archive.reconfigure :default @07:27:58.07 Lysis1.setRawPosition! 50 @07:27:58.12 Lysis1.seek :home @07:27:58.21 Air.setRawPosition! 50 @07:27:58.26 Air.seek :home @07:27:58.35 Plunge.setRawPosition! 50 @07:27:58.37 Lysis2.setRawPosition! 50 @07:27:58.46 Plunge.seek :home @07:27:58.49 Lysis2.seek :home @07:27:58.65 Lysis1.reconfigure :default @07:27:58.68 Air.reconfigure :default @07:27:58.81 Plunge.reconfigure :default @07:27:58.89 Lysis2.reconfigure :default @07:27:59.11 Toroid.setRawPosition! 0 @07:27:59.15 Toroid.seek :home @07:27:59.60 Toroid.reconfigure :default @07:27:59.73 Toroid.seek :clear @07:28:01.37 Carousel.reconfigure :default @07:28:01.44 Carousel.home.jog 0 @07:28:01.93 Carousel.seek 16 @07:28:02.07 Selecting Cartridge 17 @07:28:02.31 Carousel.seek 17 @07:28:02.79 Lysis1.seek :locked @07:28:06.93 Plunge.seek :locked @07:28:07.05 Air.seek :locked @07:28:11.18 Powering cartridge bus @07:28:13.31 Gate.setIR 1750,5000,0,6 @07:28:13.43 Gate.setIR 0,5000,0,6 @07:28:13.47 Loaded Archive Cartridge Gate.power :cartridge,:OFF @07:28:29.24 -> Cmd.startFiltering @07:28:29.46 Duration of filtering limited to 2:05:00 @07:28:29.71 Priming sample loop w/100ml, bypass w/10ml @07:28:29.72 SamplePump.setPosition! 0ml @07:28:29.77 Bypass.open @07:28:31.29 Intake.open @07:28:32.07 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:28:34.91 Vacuum tare is 52% of full scale @07:28:35.03 Pressure.reconfigure :default @07:28:35.09 Pressure.hold @07:28:35.26 SamplePump.reconfigure :prime @07:28:35.33 SamplePump.seek 10ml,1:21 @07:28:44.28 Pressure.coast @07:28:44.33 SamplePump.setPosition! 0ml @07:28:44.38 Exhaust.open @07:28:45.80 Bypass.close @07:28:47.36 Pressure.reconfigure :default @07:28:47.42 Pressure.hold @07:28:47.64 SamplePump.reconfigure :prime @07:28:47.71 SamplePump.seek 100ml,8:51 @07:30:45.07 Pressure.coast @07:30:45.13 Bypass.open @07:30:45.87 Intake.close @07:30:46.71 Toroid.reconfigure :hold @07:30:46.78 Toroid.seek :crack @07:30:47.57 Toroid.reconfigure :default @07:30:47.63 Toroid.seek :clear @07:30:49.19 Toroid.reconfigure :hold @07:30:49.26 Toroid.seek :cartridge @07:30:51.07 Toroid.reconfigure :default @07:30:51.13 Toroid.seek :clear @07:30:53.09 Pressurizing cartridge to 26psi above 17.1psia @07:30:53.11 SamplePump.setPosition! 0ml @07:30:53.15 Intake.open @07:30:53.89 Bypass.close @07:30:54.62 Exhaust.close @07:30:55.44 Pressure.reconfigure :default @07:30:55.49 Pressure.hold @07:30:55.66 SamplePump.reconfigure :charge @07:30:55.73 SamplePump.seek 2.3ml,42 seconds @07:31:03.39 Pressure.coast @07:31:03.44 Intake.close @07:31:04.26 Pressurized cartridge with 0.274ml to 26.3psi @07:31:04.27 Isolated @40.1psia (23.0psi) @07:31:04.28 Checking for cartridge leaks from 40.1psia @07:31:04.31 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @07:31:09.35 Cartridge pressure fell 1% to 39.7psia @07:31:09.43 Sampling 1000.0ml @07:31:09.44 SamplePump.setPosition! 0ml @07:31:09.49 Exhaust.open @07:31:10.22 Intake.open @07:31:11.11 Pressure.reconfigure :default @07:31:11.17 Pressure.hold @07:31:11.28 Sea 2.2m deep (17.9psia) @07:31:11.41 SamplePump.reconfigure :sample @07:31:11.48 SamplePump.seek 1000ml,1:23:53 @07:31:14.15 Cartridge 17 sampling 1/1000ml at 0.37ml/s with 23.6psi vacuum @07:32:14.15 Cartridge 17 sampling 22/1000ml at 0.35ml/s with 27.3psi vacuum 1.6m deep (17.1psia) @07:33:14.15 Cartridge 17 sampling 40/1000ml at 0.24ml/s with 26.9psi vacuum @07:34:14.15 Cartridge 17 sampling 53/1000ml at 0.20ml/s with 29.1psi vacuum @07:34:21.13 Flow averaged 0.30ml/s over 3:03 @07:34:21.16 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @07:34:21.86 Relieved excess vacuum in 7.6 seconds @07:34:21.88 SamplePump.seek 1000ml,1:19:22 @07:34:23.95 Flow estimate reduced to 0.24ml/s @07:34:23.98 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @07:34:24.68 Relieved excess vacuum in 2.7 seconds @07:34:24.70 SamplePump.seek 1000ml,1:19:20 @07:34:27.46 Flow estimate reduced to 0.19ml/s @07:34:27.49 Intake.close @07:34:27.54 Exhaust.close @07:34:27.70 Toroid.reconfigure :hold @07:34:27.77 Toroid.seek :bypass @07:34:29.39 Toroid.reconfigure :default @07:34:29.46 Toroid.seek :clear @07:34:31.08 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @07:34:31.10 Pressure.coast @07:34:31.28 Sampled 55.0ml @07:34:33.24 -> Cmd.startProcessing @07:34:33.50 Plunge.seek :reagent @07:34:34.35 Sample loop closed. Ready to Deliver Reagents @07:34:34.36 Archive.seek :sealed @07:34:38.64 Archive.seek :evac @07:34:52.58 delay 1:00 @07:35:52.61 Archive.seek :clear @07:36:10.32 RNAlater soak for 10:00 @07:46:10.35 Archive.seek :tVent @07:46:12.92 delay 1 second @07:46:13.94 Archive.seek :evac @07:46:29.57 delay 10 seconds @07:46:39.60 Archive.seek :tVent @07:46:55.23 delay 1 second @07:46:56.25 Archive.seek :evac @07:47:11.88 delay 10 seconds @07:47:21.91 Sample preserved and evacuated @07:47:22.88 -> Cmd.stop @07:47:23.02 Carousel.seek 17 @07:47:23.19 Plunge.seek :locked @07:47:24.03 PinPort.seek :clear @07:47:24.17 Archive.seek :clear @07:47:24.30 Air.seek :clear @07:47:24.44 Lysis1.seek :clear @07:47:41.93 Archive.coast @07:47:41.98 Air.coast @07:47:42.04 Lysis1.coast @07:47:42.08 Lysis2.coast @07:47:42.14 Plunge.coast @07:47:42.18 SamplePump.coast @07:47:42.23 Toroid.coast @07:47:42.28 Carousel.coast @07:47:42.34 PinPort.coast @07:47:42.52 Gate.power :motor,:OFF @07:47:42.53 Gate.power :core,:OFF @07:47:42.56 Gate.power :analytic1,:OFF @07:47:42.58 Gate.power :analytic2,:OFF @07:47:42.60 Gate.power :analytic3,:OFF @07:47:42.62 Gate.power :main,:OFF @07:47:42.64 Safely stopped and ready to power off ##-- file closed 2019-08-25T12:03:32.022Z ##-- file opened 2019-08-25T12:03:32.024Z @08:03:32.38 -> showStatus @08:03:34.39 -> Cmd.loadCartridge -15 @08:03:34.53 Gate.power :main,:ON,limit to 5000mA @08:03:34.55 Gate.power :analytic1,:ON,limit to 5000mA @08:03:34.57 Gate.power :analytic2,:ON,limit to 5000mA @08:03:34.59 Gate.power :analytic3,:ON,limit to 5000mA @08:03:34.72 Powering core bus @08:03:37.00 SPR Module 1, FW Rev. B, FPGA Rev. C @08:03:37.13 Archive.configure :default @08:03:37.25 Air.configure :default @08:03:37.35 Lysis1.configure :default @08:03:37.45 Lysis2.configure :default @08:03:37.57 Plunge.configure :default @08:03:37.69 SamplePump.configure :default @08:03:37.81 ExtraPump.configure :default @08:03:37.97 Toroid.configure :default @08:03:38.09 Carousel.configure :default @08:03:38.21 Pressure.configure :default @08:03:38.31 PinPort.configure :default @08:03:38.36 All elf controllers running firmware version 4.58 @08:03:38.39 Gate.power :motor,:ON,limit to 5000mA @08:03:38.56 Archive.reconfigure :default @08:03:38.82 Archive.home.jog 0 @08:03:38.95 Toroid.reconfigure :default @08:03:39.03 Plunge.reconfigure :default @08:03:39.07 Lysis1.reconfigure :default @08:03:39.10 PinPort.reconfigure :default @08:03:39.16 Air.reconfigure :default @08:03:39.20 Lysis2.reconfigure :default @08:03:39.45 Air.home.jog 0 @08:03:39.49 Lysis1.home.jog 0 @08:03:39.55 PinPort.home.jog 0 @08:03:39.61 Lysis2.home.jog 0 @08:03:39.67 Toroid.home.jog 0 @08:03:39.73 Plunge.home.jog 0 @08:03:40.02 Archive.reconfigure :homing @08:03:40.13 Archive.jog 1361 @08:03:40.30 Toroid.reconfigure :homing @08:03:40.33 Plunge.reconfigure :homing @08:03:40.37 Air.reconfigure :homing @08:03:40.40 PinPort.reconfigure :homing @08:03:40.45 Lysis1.reconfigure :homing @08:03:40.51 Lysis2.reconfigure :homing @08:03:40.69 Lysis1.jog 1402 @08:03:40.75 Toroid.jog 629 @08:03:40.81 Air.jog 1408 @08:03:40.86 Plunge.jog 1546 @08:03:40.92 PinPort.jog -107 @08:03:40.98 Lysis2.jog 1422 @08:03:41.16 PinPort.setRawPosition! 0 @08:03:41.20 PinPort.seek :home @08:03:41.30 PinPort.reconfigure :default @08:03:41.33 Archive.setRawPosition! 50 @08:03:41.41 Archive.seek :home @08:03:41.70 Archive.reconfigure :default @08:03:41.85 Lysis1.setRawPosition! 50 @08:03:41.89 Lysis1.seek :home @08:03:41.99 Air.setRawPosition! 50 @08:03:42.04 Air.seek :home @08:03:42.15 Plunge.setRawPosition! 50 @08:03:42.18 Lysis2.setRawPosition! 50 @08:03:42.24 Plunge.seek :home @08:03:42.28 Lysis2.seek :home @08:03:42.44 Air.reconfigure :default @08:03:42.46 Lysis1.reconfigure :default @08:03:42.60 Plunge.reconfigure :default @08:03:42.68 Lysis2.reconfigure :default @08:03:42.89 Toroid.setRawPosition! 0 @08:03:43.29 Toroid.seek :home @08:03:43.37 Toroid.reconfigure :default @08:03:43.51 Toroid.seek :clear @08:03:45.13 Carousel.reconfigure :default @08:03:45.21 Carousel.home.jog 0 @08:03:45.70 Carousel.seek 17 @08:03:45.86 Selecting Cartridge 14 @08:03:46.10 Carousel.seek 14 @08:03:47.00 Lysis1.seek :locked @08:03:51.13 Plunge.seek :locked @08:03:51.25 Air.seek :locked @08:03:55.38 Powering cartridge bus @08:03:57.51 Gate.setIR 1750,5000,0,6 @08:03:57.63 Gate.setIR 0,5000,0,6 @08:03:57.68 Loaded Lyse-n-Go Cartridge @08:03:57.70 PuckHeater.configure HeatConfig @08:03:57.75 Gate.power :heat,:ON,limit to 5000mA @08:04:13.91 -> Cmd.startFiltering @08:04:14.13 Duration of filtering limited to 2:05:00 @08:04:14.38 Priming sample loop w/100ml, bypass w/10ml @08:04:14.39 SamplePump.setPosition! 0ml @08:04:14.43 Bypass.open @08:04:15.91 Intake.open @08:04:16.74 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:04:19.58 Vacuum tare is 52% of full scale @08:04:19.71 Pressure.reconfigure :default @08:04:19.77 Pressure.hold @08:04:19.93 SamplePump.reconfigure :prime @08:04:20.01 SamplePump.seek 10ml,1:21 @08:04:28.96 Pressure.coast @08:04:29.01 SamplePump.setPosition! 0ml @08:04:29.06 Exhaust.open @08:04:30.49 Bypass.close @08:04:32.00 Pressure.reconfigure :default @08:04:32.06 Pressure.hold @08:04:32.28 SamplePump.reconfigure :prime @08:04:32.35 SamplePump.seek 100ml,8:51 @08:06:29.54 Pressure.coast @08:06:29.60 Bypass.open @08:06:30.29 Intake.close @08:06:31.13 Toroid.reconfigure :hold @08:06:31.19 Toroid.seek :crack @08:06:31.99 Toroid.reconfigure :default @08:06:32.05 Toroid.seek :clear @08:06:33.61 Toroid.reconfigure :hold @08:06:33.68 Toroid.seek :cartridge @08:06:35.50 Toroid.reconfigure :default @08:06:35.56 Toroid.seek :clear @08:06:37.52 Pressurizing cartridge to 26psi above 13.2psia @08:06:37.54 SamplePump.setPosition! 0ml @08:06:37.58 Intake.open @08:06:38.33 Bypass.close @08:06:39.02 Exhaust.close @08:06:39.84 Pressure.reconfigure :default @08:06:39.89 Pressure.hold @08:06:40.06 SamplePump.reconfigure :charge @08:06:40.13 SamplePump.seek 2.3ml,42 seconds @08:06:50.04 Pressure.coast @08:06:50.09 Intake.close @08:06:50.94 Pressurized cartridge with 0.460ml to 26.2psi @08:06:50.95 Isolated @34.8psia (21.6psi) @08:06:50.96 Checking for cartridge leaks from 34.8psia @08:06:50.99 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @08:06:56.03 Cartridge pressure fell 1% to 34.4psia @08:06:56.11 Sampling 1000.0ml @08:06:56.12 SamplePump.setPosition! 0ml @08:06:56.17 Exhaust.open @08:06:56.88 Intake.open @08:06:57.78 Pressure.reconfigure :default @08:06:57.84 Pressure.hold @08:06:58.06 SamplePump.reconfigure :sample @08:06:58.14 SamplePump.seek 1000ml,1:23:53 @08:07:00.82 Cartridge 14 sampling 1/1000ml at 0.52ml/s with 24.3psi vacuum @08:08:00.82 Cartridge 14 sampling 32/1000ml at 0.50ml/s with 26.4psi vacuum @08:09:00.81 Cartridge 14 sampling 60/1000ml at 0.43ml/s with 26.7psi vacuum @08:10:01.20 Cartridge 14 sampling 83/1000ml at 0.34ml/s with 22.5psi vacuum @08:11:00.82 Cartridge 14 sampling 103/1000ml at 0.29ml/s with 22.6psi vacuum @08:12:00.82 Cartridge 14 sampling 119/1000ml at 0.25ml/s with 22.9psi vacuum @08:13:00.82 Cartridge 14 sampling 133/1000ml at 0.20ml/s with 25.5psi vacuum @08:14:00.81 Cartridge 14 sampling 145/1000ml at 0.20ml/s with 24.1psi vacuum @08:14:12.91 Flow averaged 0.34ml/s over 7:13 @08:14:12.94 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi @08:14:13.64 Relieved excess vacuum in 2.7 seconds @08:14:13.66 SamplePump.seek 1000ml,1:11:35 @08:14:15.42 Flow estimate reduced to 0.27ml/s @08:14:15.46 Waiting up to 10 seconds for vacuum to reduce from 27.0 to 18.0psi @08:14:16.46 Relieved excess vacuum in 2.7 seconds @08:14:16.48 SamplePump.seek 1000ml,1:11:34 @08:14:26.13 Flow estimate reduced to 0.22ml/s @08:14:26.17 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @08:14:26.87 Relieved excess vacuum in 7.3 seconds @08:14:26.89 SamplePump.seek 1000ml,1:11:24 @08:14:28.65 Flow estimate reduced to 0.17ml/s @08:14:28.66 Intake.close @08:14:28.71 Exhaust.close @08:14:28.90 Toroid.reconfigure :hold @08:14:28.97 Toroid.seek :bypass @08:14:30.58 Toroid.reconfigure :default @08:14:30.65 Toroid.seek :clear @08:14:32.27 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @08:14:32.29 Pressure.coast @08:14:32.47 Sampled 150.0ml @08:14:34.45 -> Cmd.startProcessing @08:14:36.29 SPR.command \"read_sequences\" @08:14:36.32 SPR.command \"stats_on\" @08:14:37.09 SPR.command \"start 0 1\" @08:14:39.10 Begun log /var/log/esp/spr19aug2508h.spr @08:14:39.94 Handoff.select :waste @08:14:40.12 Archive.seek :empty @08:14:47.33 Syringe tare is 21% of full scale @08:14:57.85 PinPort.seek :cartridge @08:15:02.41 Lysis1.seek 1350ul @08:15:04.88 Lysis2.seek 1700ul @08:15:07.97 Plunge.seek :reagent @08:15:08.82 Evacuating puck with filter bubble point of 50psi @08:15:08.84 PinPort.seek :cartridge @08:15:08.92 Handoff.select :waste @08:15:17.38 Air pumping 1 strokes to reach 8psi @08:15:17.40 Air.reconfigure :gulp @08:15:17.48 Air.seek :tVent @08:15:19.28 0.2psi after gulp @08:15:19.29 Air stroke #1 of 1 @08:15:19.30 Air.reconfigure :pressurize @08:15:19.38 Air.seek :pressurized @08:15:32.22 0.2psi after pressurize @08:15:32.23 Air.reconfigure :compress @08:15:32.31 Air.seek :evac @08:15:35.03 0.7psi after compress @08:15:48.90 0.3psi after compress (stable) @08:15:49.02 at 0.2psi Opened to waste @08:15:49.03 Handoff.select :blocked @08:15:49.21 Air pumping 10 strokes to reach 50psi @08:15:49.22 Air.reconfigure :gulp @08:15:49.30 Air.seek :tVent @08:15:59.58 8.0psi after gulp @08:15:59.59 Air stroke #1 of 10 @08:15:59.61 Air.reconfigure :pressurize @08:15:59.68 Air.seek :pressurized @08:16:12.53 8.7psi after pressurize @08:16:12.54 Air.reconfigure :compress @08:16:12.61 Air.seek :evac @08:16:15.28 12.4psi after compress @08:16:35.82 23.2psi after compress (stable) @08:16:35.85 Air.reconfigure :gulp @08:16:35.93 Air.seek :tVent @08:16:45.82 23.4psi after gulp @08:16:45.83 Air stroke #2 of 10 @08:16:45.85 Air.reconfigure :pressurize @08:16:45.93 Air.seek :pressurized @08:16:58.76 23.0psi after pressurize @08:16:58.77 Air.reconfigure :compress @08:16:58.85 Air.seek :evac @08:17:01.52 27.0psi after compress @08:17:19.31 34.8psi after compress (stable) @08:17:19.34 Air.reconfigure :gulp @08:17:19.42 Air.seek :tVent @08:17:29.32 35.1psi after gulp @08:17:29.33 Air stroke #3 of 10 @08:17:29.35 Air.reconfigure :pressurize @08:17:29.43 Air.seek :pressurized @08:17:42.27 34.5psi after pressurize @08:17:42.28 Air.reconfigure :compress @08:17:42.36 Air.seek :evac @08:17:45.02 37.7psi after compress @08:18:02.09 44.2psi after compress (stable) @08:18:02.11 Air.reconfigure :gulp @08:18:02.18 Air.seek :tVent @08:18:12.08 44.6psi after gulp @08:18:12.09 Air stroke #4 of 10 @08:18:12.11 Air.reconfigure :pressurize @08:18:12.19 Air.seek :pressurized @08:18:25.03 44.0psi after pressurize @08:18:25.04 Air.reconfigure :compress @08:18:25.12 Air.seek :evac @08:18:27.80 46.1psi after compress @08:18:30.06 Reached 50.1 of 50.0psi after pumping 7787ul (5.00 strokes) @08:18:30.07 Handoff.select :waste @08:18:51.80 delay 30 seconds @08:19:21.82 Handoff.select :blocked @08:19:46.86 Handoff.select :waste @08:19:46.98 PSI at @1.37psi at evacEnd @08:19:46.99 Handoff.select :blocked @08:19:47.11 Air.reconfigure :gulp @08:19:47.22 Air.seek :tVent @08:20:04.33 Stable @1.2psi after Handoff blocked @08:20:04.41 Air pumping 10 strokes to reach 31psi @08:20:04.42 Air stroke #1 of 10 @08:20:04.83 Air.reconfigure :pressurize @08:20:04.91 Air.seek :pressurized @08:20:17.74 1.3psi after pressurize @08:20:17.76 Air.reconfigure :compress @08:20:17.83 Air.seek :evac @08:20:20.50 3.9psi after compress @08:20:41.59 11.0psi after compress (stable) @08:20:41.61 Air.reconfigure :gulp @08:20:41.68 Air.seek :tVent @08:20:51.61 11.3psi after gulp @08:20:51.62 Air stroke #2 of 10 @08:20:51.64 Air.reconfigure :pressurize @08:20:51.72 Air.seek :pressurized @08:21:04.56 11.4psi after pressurize @08:21:04.57 Air.reconfigure :compress @08:21:04.65 Air.seek :evac @08:21:07.34 11.4psi after compress @08:21:25.69 19.6psi after compress (stable) @08:21:25.71 Air.reconfigure :gulp @08:21:25.78 Air.seek :tVent @08:21:35.70 20.0psi after gulp @08:21:35.71 Air stroke #3 of 10 @08:21:35.73 Air.reconfigure :pressurize @08:21:35.81 Air.seek :pressurized @08:21:48.65 19.9psi after pressurize @08:21:48.66 Air.reconfigure :compress @08:21:48.74 Air.seek :evac @08:21:51.40 19.9psi after compress @08:22:10.45 27.6psi after compress (stable) @08:22:10.47 Air.reconfigure :gulp @08:22:10.55 Air.seek :tVent @08:22:20.43 27.9psi after gulp @08:22:20.50 Air stroke #4 of 10 @08:22:20.52 Air.reconfigure :pressurize @08:22:20.59 Air.seek :pressurized @08:22:33.42 27.8psi after pressurize @08:22:33.50 Air.reconfigure :compress @08:22:33.57 Air.seek :evac @08:22:36.27 28.8psi after compress @08:22:37.75 Reached 31.4 of 31.2psi after pumping 7787ul (5.00 strokes) @08:22:37.76 Handoff.select :waste @08:22:50.08 delay 30 seconds @08:23:20.10 Handoff.select :blocked @08:23:45.10 Handoff.select :waste @08:23:45.23 PSI at @0.53psi at evacEnd @08:23:45.24 Air.reconfigure :default @08:23:45.31 Air.seek :fVent @08:23:46.76 delay 5 seconds @08:23:51.78 Seawater evacuated, Presure above/below filter released. Lysis begins @08:23:51.86 Lysis1.reconfigure :slow @08:23:51.94 Lysis1.seek 1050ul @08:24:21.96 delay 5 seconds @08:24:26.98 Handoff.select :blocked @08:24:27.14 Lysis1.seek 450ul @08:25:27.10 delay 3 seconds @08:25:30.18 Air.reconfigure to mix into 1.37806 parts Lysis1 @08:25:30.60 Air.seek :evac @08:25:30.64 Lysis1.seek 300ul @08:25:45.75 Air.reconfigure :default @08:25:45.81 Air.seek :full @08:26:00.01 Lysis1.seek :empty @08:26:30.09 Lysis Delivered. Syringe pressure @26.7psi @08:26:30.13 PuckHeater.seek 84.4C @08:27:19.70 PuckHeater.hold 15:00,84.4C @08:42:15.97 Lysis complete. Syringe pressure @35.7psi @08:42:15.98 Handoff.select :blocked @08:42:16.08 Lysis1.reconfigure :halfSpeed @08:42:16.14 Lysis1.seek 200ul @08:42:19.91 delay 1 second @08:42:20.93 Lysis1.seek :empty @08:42:24.76 Initial AirS pressure @34.4psi @08:42:24.82 Lysis1.reconfigure :extract @08:42:24.91 Lysis1.seek 900ul,10:00 @08:42:26.09 Air pumping 10 strokes to reach 21psi @08:42:26.10 Air stroke #1 of 10 @08:42:26.12 Air.reconfigure :pressurize @08:42:26.21 Air.seek :pressurized @08:42:39.03 16.6psi after pressurize @08:42:39.05 Air.reconfigure :compress @08:42:39.12 Air.seek :evac @08:42:41.79 16.7psi after compress @08:42:56.09 15.8psi after compress (stable) @08:42:56.11 Air.reconfigure :gulp @08:42:56.19 Air.seek :tVent @08:43:06.07 16.1psi after gulp @08:43:06.08 Air stroke #2 of 10 @08:43:06.10 Air.reconfigure :pressurize @08:43:06.18 Air.seek :pressurized @08:43:19.02 16.1psi after pressurize @08:43:19.03 Air.reconfigure :compress @08:43:19.11 Air.seek :evac @08:43:21.78 16.1psi after compress @08:43:25.35 Air.reconfigure :default @08:43:25.41 Air.hold @08:43:25.58 Lysis1 at 900ul @16.8psi @08:43:25.61 Lysis1.reconfigure :default @08:43:25.69 Lysis1.seek 925ul @08:43:26.41 Syringe::Overpressure in PROCESSING -- 17.5psi after recovery pull -- Skipping SPR @08:43:26.43 SPR.command \"start 2 14\" @08:43:27.03 Handoff.select :waste @08:43:27.50 Ended log /var/log/esp/spr19aug2508h.spr @08:43:27.51 SPR.command \"stats_off\" @08:43:29.94 -> Cmd.stop @08:43:30.03 Handoff.select :close @08:43:30.16 Gate.power :heat,:OFF @08:43:30.18 Gate.power :cartridge,:OFF @08:43:30.26 Carousel.seek 14 @08:43:30.44 Plunge.seek :locked @08:43:31.27 PinPort.seek :clear @08:43:35.88 Archive.seek :clear @08:43:36.01 Air.seek :clear @08:43:36.14 Lysis1.seek :clear @08:43:36.28 Lysis2.seek :clear @08:43:53.62 Archive.coast @08:43:53.67 Air.coast @08:43:53.72 Lysis1.coast @08:43:53.77 Lysis2.coast @08:43:53.82 Plunge.coast @08:43:53.87 SamplePump.coast @08:43:53.92 Toroid.coast @08:43:53.97 Carousel.coast @08:43:54.02 PinPort.coast @08:43:54.21 Gate.power :motor,:OFF @08:43:54.23 Gate.power :core,:OFF @08:43:54.25 Gate.power :analytic1,:OFF @08:43:54.27 Gate.power :analytic2,:OFF @08:43:54.29 Gate.power :analytic3,:OFF @08:43:54.31 Gate.power :main,:OFF @08:43:54.33 Safely stopped and ready to power off ##-- file closed 2019-08-25T15:12:36.634Z ##-- file opened 2019-08-25T15:12:36.635Z @11:12:36.71 -> showStatus @11:12:38.73 -> Cmd.loadCartridge -4 @11:12:38.87 Gate.power :main,:ON,limit to 5000mA @11:12:38.89 Gate.power :analytic1,:ON,limit to 5000mA @11:12:38.91 Gate.power :analytic2,:ON,limit to 5000mA @11:12:38.94 Gate.power :analytic3,:ON,limit to 5000mA @11:12:39.07 Powering core bus @11:12:41.34 SPR Module 1, FW Rev. B, FPGA Rev. C @11:12:41.47 Archive.configure :default @11:12:41.59 Air.configure :default @11:12:41.69 Lysis1.configure :default @11:12:41.79 Lysis2.configure :default @11:12:41.94 Plunge.configure :default @11:12:42.06 SamplePump.configure :default @11:12:42.18 ExtraPump.configure :default @11:12:42.30 Toroid.configure :default @11:12:42.42 Carousel.configure :default @11:12:42.54 Pressure.configure :default @11:12:42.64 PinPort.configure :default @11:12:42.70 All elf controllers running firmware version 4.58 @11:12:42.73 Gate.power :motor,:ON,limit to 5000mA @11:12:42.95 Archive.reconfigure :default @11:12:43.17 Archive.home.jog 0 @11:12:43.30 Toroid.reconfigure :default @11:12:43.38 Plunge.reconfigure :default @11:12:43.42 Lysis1.reconfigure :default @11:12:43.45 PinPort.reconfigure :default @11:12:43.50 Air.reconfigure :default @11:12:43.55 Lysis2.reconfigure :default @11:12:43.75 Air.home.jog 0 @11:12:43.80 Plunge.home.jog 0 @11:12:43.86 PinPort.home.jog 0 @11:12:43.92 Lysis1.home.jog 0 @11:12:43.97 Lysis2.home.jog 0 @11:12:44.03 Toroid.home.jog 0 @11:12:44.56 Toroid.reconfigure :homing @11:12:44.58 Plunge.reconfigure :homing @11:12:44.61 Air.reconfigure :homing @11:12:44.65 Archive.reconfigure :homing @11:12:44.69 PinPort.reconfigure :homing @11:12:44.72 Lysis1.reconfigure :homing @11:12:44.78 Lysis2.reconfigure :homing @11:12:45.00 Lysis1.jog 1397 @11:12:45.06 Air.jog 1406 @11:12:45.11 Plunge.jog 1547 @11:12:45.16 Lysis2.jog 1429 @11:12:45.22 Toroid.jog 621 @11:12:45.28 Archive.jog 1378 @11:12:45.34 PinPort.jog -108 @11:12:45.57 PinPort.setRawPosition! 0 @11:12:45.61 PinPort.seek :home @11:12:45.71 PinPort.reconfigure :default @11:12:46.15 Lysis1.setRawPosition! 50 @11:12:46.20 Lysis1.seek :home @11:12:46.28 Air.setRawPosition! 50 @11:12:46.34 Air.seek :home @11:12:46.44 Plunge.setRawPosition! 50 @11:12:46.47 Lysis2.setRawPosition! 50 @11:12:46.49 Archive.setRawPosition! 50 @11:12:46.57 Plunge.seek :home @11:12:46.64 Archive.seek :home @11:12:46.68 Lysis2.seek :home @11:12:46.85 Air.reconfigure :default @11:12:46.87 Lysis1.reconfigure :default @11:12:47.03 Plunge.reconfigure :default @11:12:47.11 Archive.reconfigure :default @11:12:47.13 Lysis2.reconfigure :default @11:12:47.64 Toroid.setRawPosition! 0 @11:12:47.68 Toroid.seek :home @11:12:47.79 Toroid.reconfigure :default @11:12:47.93 Toroid.seek :clear @11:12:49.56 Carousel.reconfigure :default @11:12:49.63 Carousel.home.jog 0 @11:12:50.13 Carousel.seek 14 @11:12:50.29 Selecting Cartridge 15 @11:12:50.53 Carousel.seek 15 @11:12:51.02 Lysis1.seek :locked @11:12:55.15 Plunge.seek :locked @11:12:55.27 Air.seek :locked @11:12:59.40 Powering cartridge bus @11:13:01.52 Gate.setIR 1750,5000,0,6 @11:13:01.65 Gate.setIR 0,5000,0,6 @11:13:01.69 Loaded Archive Cartridge Gate.power :cartridge,:OFF @11:13:17.30 -> Cmd.startFiltering @11:13:17.53 Duration of filtering limited to 2:05:00 @11:13:17.77 Priming sample loop w/100ml, bypass w/10ml @11:13:17.78 SamplePump.setPosition! 0ml @11:13:17.83 Bypass.open @11:13:19.30 Intake.open @11:13:20.08 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:13:22.92 Vacuum tare is 52% of full scale @11:13:23.05 Pressure.reconfigure :default @11:13:23.11 Pressure.hold @11:13:23.28 SamplePump.reconfigure :prime @11:13:23.35 SamplePump.seek 10ml,1:21 @11:13:32.30 Pressure.coast @11:13:32.35 SamplePump.setPosition! 0ml @11:13:32.40 Exhaust.open @11:13:33.84 Bypass.close @11:13:35.39 Pressure.reconfigure :default @11:13:35.45 Pressure.hold @11:13:35.67 SamplePump.reconfigure :prime @11:13:35.74 SamplePump.seek 100ml,8:51 @11:15:32.75 Pressure.coast @11:15:32.81 Bypass.open @11:15:33.54 Intake.close @11:15:34.35 Toroid.reconfigure :hold @11:15:34.41 Toroid.seek :crack @11:15:35.21 Toroid.reconfigure :default @11:15:35.27 Toroid.seek :clear @11:15:36.83 Toroid.reconfigure :hold @11:15:36.90 Toroid.seek :cartridge @11:15:38.71 Toroid.reconfigure :default @11:15:38.77 Toroid.seek :clear @11:15:40.74 Pressurizing cartridge to 26psi above 12.7psia @11:15:40.75 SamplePump.setPosition! 0ml @11:15:40.79 Intake.open @11:15:41.54 Bypass.close @11:15:42.27 Exhaust.close @11:15:43.10 Pressure.reconfigure :default @11:15:43.15 Pressure.hold @11:15:43.32 SamplePump.reconfigure :charge @11:15:43.40 SamplePump.seek 2.3ml,42 seconds @11:15:53.54 Pressure.coast @11:15:53.59 Intake.close @11:15:54.42 Pressurized cartridge with 0.483ml to 26.1psi @11:15:54.43 Isolated @34.9psia (22.2psi) @11:15:54.44 Checking for cartridge leaks from 34.9psia @11:15:54.47 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @11:15:59.51 Cartridge pressure fell 1% to 34.6psia @11:15:59.59 Sampling 1000.0ml @11:15:59.60 SamplePump.setPosition! 0ml @11:15:59.65 Exhaust.open @11:16:00.38 Intake.open @11:16:01.22 Pressure.reconfigure :default @11:16:01.28 Pressure.hold @11:16:01.49 SamplePump.reconfigure :sample @11:16:01.57 SamplePump.seek 1000ml,1:23:53 @11:16:04.26 Cartridge 15 sampling 1/1000ml at 0.29ml/s with 23.1psi vacuum @11:16:59.17 Flow averaged 0.26ml/s over 54 seconds @11:16:59.20 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @11:17:00.59 Relieved excess vacuum in 5.8 seconds @11:17:00.61 SamplePump.seek 1000ml,1:22:42 @11:17:02.63 Flow estimate reduced to 0.21ml/s @11:17:02.67 Waiting up to 17 seconds for vacuum to reduce from 29.6 to 18.0psi @11:17:03.67 Relieved excess vacuum in 2.9 seconds @11:17:03.69 SamplePump.seek 1000ml,1:22:40 @11:17:04.26 Cartridge 15 sampling 15/1000ml at 0.21ml/s with 12.6psi vacuum @11:17:05.66 Flow estimate reduced to 0.17ml/s @11:17:05.69 Intake.close @11:17:05.72 Exhaust.close @11:17:05.90 Toroid.reconfigure :hold @11:17:05.98 Toroid.seek :bypass @11:17:07.60 Toroid.reconfigure :default @11:17:07.67 Toroid.seek :clear @11:17:09.30 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:17:09.32 Pressure.coast @11:17:09.50 Sampled 14.9ml @11:17:11.71 -> Cmd.startProcessing @11:17:11.96 Plunge.seek :reagent @11:17:12.81 Sample loop closed. Ready to Deliver Reagents @11:17:12.82 Archive.seek :sealed @11:17:17.09 Archive.seek :evac @11:17:31.03 delay 1:00 @11:18:31.05 Archive.seek :clear @11:18:48.76 RNAlater soak for 10:00 @11:28:48.79 Archive.seek :tVent @11:28:51.36 delay 1 second @11:28:52.38 Archive.seek :evac @11:29:08.01 delay 10 seconds @11:29:18.04 Archive.seek :tVent @11:29:33.66 delay 1 second @11:29:34.68 Archive.seek :evac @11:29:50.30 delay 10 seconds @11:30:00.33 Sample preserved and evacuated @11:30:01.52 -> Cmd.stop @11:30:01.66 Carousel.seek 15 @11:30:01.83 Plunge.seek :locked @11:30:02.67 PinPort.seek :clear @11:30:02.81 Archive.seek :clear @11:30:02.94 Air.seek :clear @11:30:03.07 Lysis1.seek :clear @11:30:20.56 Archive.coast @11:30:20.61 Air.coast @11:30:20.67 Lysis1.coast @11:30:20.72 Lysis2.coast @11:30:20.76 Plunge.coast @11:30:20.82 SamplePump.coast @11:30:20.87 Toroid.coast @11:30:20.92 Carousel.coast @11:30:20.96 PinPort.coast @11:30:21.14 Gate.power :motor,:OFF @11:30:21.16 Gate.power :core,:OFF @11:30:21.18 Gate.power :analytic1,:OFF @11:30:21.20 Gate.power :analytic2,:OFF @11:30:21.22 Gate.power :analytic3,:OFF @11:30:21.24 Gate.power :main,:OFF @11:30:21.26 Safely stopped and ready to power off ##-- file closed 2019-08-25T15:46:09.717Z ##-- file opened 2019-08-25T15:46:09.719Z @11:46:10.23 -> showStatus @11:46:12.25 -> Cmd.loadCartridge -15 @11:46:12.39 Gate.power :main,:ON,limit to 5000mA @11:46:12.40 Gate.power :analytic1,:ON,limit to 5000mA @11:46:12.42 Gate.power :analytic2,:ON,limit to 5000mA @11:46:12.45 Gate.power :analytic3,:ON,limit to 5000mA @11:46:12.59 Powering core bus @11:46:14.86 SPR Module 1, FW Rev. B, FPGA Rev. C @11:46:14.99 Archive.configure :default @11:46:15.11 Air.configure :default @11:46:15.21 Lysis1.configure :default @11:46:15.31 Lysis2.configure :default @11:46:15.43 Plunge.configure :default @11:46:15.55 SamplePump.configure :default @11:46:15.67 ExtraPump.configure :default @11:46:15.79 Toroid.configure :default @11:46:15.91 Carousel.configure :default @11:46:16.07 Pressure.configure :default @11:46:16.17 PinPort.configure :default @11:46:16.22 All elf controllers running firmware version 4.58 @11:46:16.25 Gate.power :motor,:ON,limit to 5000mA @11:46:16.45 Archive.reconfigure :default @11:46:16.69 Archive.home.jog 0 @11:46:16.82 Toroid.reconfigure :default @11:46:16.90 Plunge.reconfigure :default @11:46:16.94 Lysis1.reconfigure :default @11:46:17.00 Air.reconfigure :default @11:46:17.05 Lysis2.reconfigure :default @11:46:17.11 PinPort.reconfigure :default @11:46:17.31 PinPort.home.jog 0 @11:46:17.36 Plunge.home.jog 0 @11:46:17.42 Lysis2.home.jog 0 @11:46:17.48 Toroid.home.jog 0 @11:46:17.54 Lysis1.home.jog 0 @11:46:17.60 Air.home.jog 0 @11:46:17.86 Archive.reconfigure :homing @11:46:18.07 Toroid.reconfigure :homing @11:46:18.11 Plunge.reconfigure :homing @11:46:18.16 PinPort.reconfigure :homing @11:46:18.22 Lysis1.reconfigure :homing @11:46:18.25 Lysis2.reconfigure :homing @11:46:18.43 Toroid.jog 625 @11:46:18.48 PinPort.jog -107 @11:46:18.54 Lysis1.jog 1399 @11:46:18.60 Plunge.jog 1544 @11:46:18.66 Archive.jog 1383 @11:46:18.72 Lysis2.jog 1425 @11:46:18.82 PinPort.setRawPosition! 0 @11:46:18.83 Air.reconfigure :homing @11:46:18.93 PinPort.seek :home @11:46:19.01 Air.jog 1396 @11:46:19.09 PinPort.reconfigure :default @11:46:19.70 Lysis1.setRawPosition! 50 @11:46:19.74 Lysis1.seek :home @11:46:19.85 Plunge.setRawPosition! 50 @11:46:19.87 Archive.setRawPosition! 50 @11:46:19.94 Plunge.seek :home @11:46:19.98 Archive.seek :home @11:46:20.12 Lysis1.reconfigure :default @11:46:20.17 Lysis2.setRawPosition! 50 @11:46:20.24 Lysis2.seek :home @11:46:20.36 Plunge.reconfigure :default @11:46:20.39 Air.setRawPosition! 50 @11:46:20.41 Archive.reconfigure :default @11:46:20.52 Air.seek :home @11:46:21.03 Toroid.setRawPosition! 0 @11:46:21.05 Lysis2.reconfigure :default @11:46:21.13 Toroid.seek :home @11:46:21.28 Toroid.reconfigure :default @11:46:21.30 Air.reconfigure :default @11:46:21.71 Toroid.seek :clear @11:46:23.34 Carousel.reconfigure :default @11:46:23.41 Carousel.home.jog 0 @11:46:23.89 Carousel.seek 15 @11:46:24.05 Selecting Cartridge 12 @11:46:24.30 Carousel.seek 12 @11:46:25.19 Lysis1.seek :locked @11:46:29.32 Plunge.seek :locked @11:46:29.44 Air.seek :locked @11:46:33.60 Powering cartridge bus @11:46:35.74 Gate.setIR 1750,5000,0,6 @11:46:35.86 Gate.setIR 0,5000,0,6 @11:46:35.91 Loaded Lyse-n-Go Cartridge @11:46:35.93 PuckHeater.configure HeatConfig @11:46:35.99 Gate.power :heat,:ON,limit to 5000mA @11:46:51.75 -> Cmd.startFiltering @11:46:51.98 Duration of filtering limited to 2:05:00 @11:46:52.23 Priming sample loop w/100ml, bypass w/10ml @11:46:52.24 SamplePump.setPosition! 0ml @11:46:52.29 Bypass.open @11:46:53.76 Intake.open @11:46:54.57 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:46:57.40 Vacuum tare is 52% of full scale @11:46:57.53 Pressure.reconfigure :default @11:46:57.59 Pressure.hold @11:46:57.76 SamplePump.reconfigure :prime @11:46:57.83 SamplePump.seek 10ml,1:21 @11:47:06.77 Pressure.coast @11:47:06.82 SamplePump.setPosition! 0ml @11:47:06.87 Exhaust.open @11:47:08.32 Bypass.close @11:47:09.85 Pressure.reconfigure :default @11:47:09.91 Pressure.hold @11:47:10.13 SamplePump.reconfigure :prime @11:47:10.20 SamplePump.seek 100ml,8:51 @11:49:07.25 Pressure.coast @11:49:07.31 Bypass.open @11:49:08.02 Intake.close @11:49:08.88 Toroid.reconfigure :hold @11:49:08.94 Toroid.seek :crack @11:49:09.74 Toroid.reconfigure :default @11:49:09.80 Toroid.seek :clear @11:49:11.36 Toroid.reconfigure :hold @11:49:11.43 Toroid.seek :cartridge @11:49:13.24 Toroid.reconfigure :default @11:49:13.31 Toroid.seek :clear @11:49:15.28 Pressurizing cartridge to 26psi above 12.5psia @11:49:15.29 SamplePump.setPosition! 0ml @11:49:15.33 Intake.open @11:49:16.09 Bypass.close @11:49:16.79 Exhaust.close @11:49:17.60 Pressure.reconfigure :default @11:49:17.65 Pressure.hold @11:49:17.82 SamplePump.reconfigure :charge @11:49:17.89 SamplePump.seek 2.3ml,42 seconds @11:49:27.45 Pressure.coast @11:49:27.51 Intake.close @11:49:28.37 Pressurized cartridge with 0.428ml to 26.0psi @11:49:28.39 Isolated @35.8psia (23.2psi) @11:49:28.41 Checking for cartridge leaks from 35.8psia @11:49:28.46 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @11:49:33.56 Sampling 1000.0ml @11:49:33.57 SamplePump.setPosition! 0ml @11:49:33.62 Exhaust.open @11:49:34.29 Intake.open @11:49:35.19 Pressure.reconfigure :default @11:49:35.25 Pressure.hold @11:49:35.47 SamplePump.reconfigure :sample @11:49:35.55 SamplePump.seek 1000ml,1:23:53 @11:49:38.23 Cartridge 12 sampling 1/1000ml at 0.50ml/s with 23.6psi vacuum @11:50:38.23 Cartridge 12 sampling 32/1000ml at 0.49ml/s with 24.6psi vacuum @11:51:38.61 Cartridge 12 sampling 59/1000ml at 0.45ml/s with 26.4psi vacuum @11:52:38.23 Cartridge 12 sampling 84/1000ml at 0.40ml/s with 23.4psi vacuum @11:53:38.23 Cartridge 12 sampling 108/1000ml at 0.38ml/s with 20.0psi vacuum @11:54:38.23 Cartridge 12 sampling 130/1000ml at 0.37ml/s with 22.9psi vacuum @11:55:38.23 Cartridge 12 sampling 150/1000ml at 0.32ml/s with 21.7psi vacuum @11:56:38.22 Cartridge 12 sampling 169/1000ml at 0.32ml/s with 23.3psi vacuum @11:57:38.23 Cartridge 12 sampling 185/1000ml at 0.26ml/s with 26.0psi vacuum @11:58:38.23 Cartridge 12 sampling 200/1000ml at 0.23ml/s with 20.6psi vacuum @11:59:38.23 Cartridge 12 sampling 213/1000ml at 0.22ml/s with 23.7psi vacuum @12:00:38.29 Cartridge 12 sampling 226/1000ml at 0.20ml/s with 27.3psi vacuum @12:01:38.22 Cartridge 12 sampling 238/1000ml at 0.20ml/s with 26.4psi vacuum @12:01:43.76 Flow averaged 0.33ml/s over 12:03 @12:01:43.79 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi @12:01:44.49 Relieved excess vacuum in 6.2 seconds @12:01:44.51 SamplePump.seek 1000ml,1:03:59 @12:01:46.32 Flow estimate reduced to 0.26ml/s @12:01:46.36 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @12:01:47.06 Relieved excess vacuum in 2.4 seconds @12:01:47.08 SamplePump.seek 1000ml,1:03:57 @12:02:04.77 Flow averaged 0.27ml/s over 14 seconds @12:02:04.80 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi @12:02:05.50 Relieved excess vacuum in 5.3 seconds @12:02:05.52 SamplePump.seek 1000ml,1:03:39 @12:02:07.25 Flow estimate reduced to 0.21ml/s @12:02:07.28 Waiting up to 15 seconds for vacuum to reduce from 28.8 to 18.0psi @12:02:08.28 Relieved excess vacuum in 2.6 seconds @12:02:08.30 SamplePump.seek 1000ml,1:03:38 @12:02:21.26 Flow estimate reduced to 0.17ml/s @12:02:21.27 Intake.close @12:02:21.34 Exhaust.close @12:02:21.51 Toroid.reconfigure :hold @12:02:21.58 Toroid.seek :bypass @12:02:23.21 Toroid.reconfigure :default @12:02:23.28 Toroid.seek :clear @12:02:24.90 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @12:02:24.92 Pressure.coast @12:02:25.10 Sampled 245.4ml @12:02:27.36 -> Cmd.startProcessing @12:02:29.21 SPR.command \"read_sequences\" @12:02:29.91 SPR.command \"stats_on\" @12:02:29.95 SPR.command \"start 0 1\" @12:02:31.96 Begun log /var/log/esp/spr19aug2512h.spr @12:02:32.76 Handoff.select :waste @12:02:32.94 Archive.seek :empty @12:02:40.12 Syringe tare is 21% of full scale @12:02:50.66 PinPort.seek :cartridge @12:02:55.22 Lysis1.seek 1350ul @12:02:57.70 Lysis2.seek 1700ul @12:03:00.80 Plunge.seek :reagent @12:03:01.64 Evacuating puck with filter bubble point of 50psi @12:03:01.66 PinPort.seek :cartridge @12:03:01.74 Handoff.select :waste @12:03:09.06 Air pumping 1 strokes to reach 8psi @12:03:09.08 Air.reconfigure :gulp @12:03:09.16 Air.seek :tVent @12:03:10.96 0.7psi after gulp @12:03:10.97 Air stroke #1 of 1 @12:03:10.99 Air.reconfigure :pressurize @12:03:11.08 Air.seek :pressurized @12:03:23.91 0.5psi after pressurize @12:03:23.93 Air.reconfigure :compress @12:03:24.00 Air.seek :evac @12:03:26.66 1.6psi after compress @12:03:40.94 0.3psi after compress (stable) @12:03:41.07 at 0.3psi Opened to waste @12:03:41.08 Handoff.select :blocked @12:03:41.25 Air pumping 10 strokes to reach 50psi @12:03:41.27 Air.reconfigure :gulp @12:03:41.36 Air.seek :tVent @12:03:51.26 9.4psi after gulp @12:03:51.27 Air stroke #1 of 10 @12:03:51.29 Air.reconfigure :pressurize @12:03:51.37 Air.seek :pressurized @12:04:04.21 11.5psi after pressurize @12:04:04.22 Air.reconfigure :compress @12:04:04.31 Air.seek :evac @12:04:06.96 13.9psi after compress @12:04:34.13 30.2psi after compress (stable) @12:04:34.15 Air.reconfigure :gulp @12:04:34.23 Air.seek :tVent @12:04:44.13 31.0psi after gulp @12:04:44.14 Air stroke #2 of 10 @12:04:44.16 Air.reconfigure :pressurize @12:04:44.24 Air.seek :pressurized @12:04:57.10 29.8psi after pressurize @12:04:57.11 Air.reconfigure :compress @12:04:57.20 Air.seek :evac @12:04:59.88 30.8psi after compress @12:05:23.21 43.8psi after compress (stable) @12:05:23.23 Air.reconfigure :gulp @12:05:23.31 Air.seek :tVent @12:05:33.22 44.4psi after gulp @12:05:33.23 Air stroke #3 of 10 @12:05:33.25 Air.reconfigure :pressurize @12:05:33.33 Air.seek :pressurized @12:05:46.16 43.1psi after pressurize @12:05:46.20 Air.reconfigure :compress @12:05:46.28 Air.seek :evac @12:05:48.95 42.7psi after compress @12:05:52.05 Reached 50.1 of 50.0psi after pumping 6230ul (4.00 strokes) @12:05:52.06 Handoff.select :waste @12:06:18.20 delay 30 seconds @12:06:48.23 Handoff.select :blocked @12:07:13.80 Handoff.select :waste @12:07:13.92 PSI at @6.14psi at evacEnd @12:07:13.93 Handoff.select :blocked @12:07:14.05 Air.reconfigure :gulp @12:07:14.16 Air.seek :tVent @12:07:31.61 Stable @4.8psi after Handoff blocked @12:07:31.70 Air pumping 10 strokes to reach 35psi @12:07:31.71 Air stroke #1 of 10 @12:07:31.73 Air.reconfigure :pressurize @12:07:31.81 Air.seek :pressurized @12:07:44.63 4.9psi after pressurize @12:07:44.65 Air.reconfigure :compress @12:07:44.72 Air.seek :evac @12:07:47.39 5.0psi after compress @12:08:16.87 16.1psi after compress (stable) @12:08:16.89 Air.reconfigure :gulp @12:08:16.97 Air.seek :tVent @12:08:26.85 16.5psi after gulp @12:08:26.86 Air stroke #2 of 10 @12:08:26.88 Air.reconfigure :pressurize @12:08:26.97 Air.seek :pressurized @12:08:39.81 16.5psi after pressurize @12:08:39.83 Air.reconfigure :compress @12:08:39.90 Air.seek :evac @12:08:42.57 17.5psi after compress @12:09:01.48 25.1psi after compress (stable) @12:09:01.50 Air.reconfigure :gulp @12:09:01.58 Air.seek :tVent @12:09:11.48 25.6psi after gulp @12:09:11.49 Air stroke #3 of 10 @12:09:11.51 Air.reconfigure :pressurize @12:09:11.60 Air.seek :pressurized @12:09:24.43 25.5psi after pressurize @12:09:24.44 Air.reconfigure :compress @12:09:24.53 Air.seek :evac @12:09:27.59 26.5psi after compress @12:09:45.94 32.7psi after compress (stable) @12:09:45.96 Air.reconfigure :gulp @12:09:46.03 Air.seek :tVent @12:09:55.94 33.3psi after gulp @12:09:56.02 Air stroke #4 of 10 @12:09:56.04 Air.reconfigure :pressurize @12:09:56.12 Air.seek :pressurized @12:10:08.94 33.2psi after pressurize @12:10:09.02 Air.reconfigure :compress @12:10:09.08 Air.seek :evac @12:10:11.78 33.7psi after compress @12:10:12.38 Reached 35.0 of 34.8psi after pumping 7787ul (5.00 strokes) @12:10:12.39 Handoff.select :waste @12:10:25.81 delay 30 seconds @12:10:55.83 Handoff.select :blocked @12:11:20.83 Handoff.select :waste @12:11:20.95 PSI at @0.48psi at evacEnd @12:11:20.97 Air.reconfigure :default @12:11:21.08 Air.seek :fVent @12:11:22.53 delay 5 seconds @12:11:27.55 Seawater evacuated, Presure above/below filter released. Lysis begins @12:11:27.63 Lysis1.reconfigure :slow @12:11:27.71 Lysis1.seek 1050ul @12:11:57.72 delay 5 seconds @12:12:02.74 Handoff.select :blocked @12:12:02.90 Lysis1.seek 450ul @12:13:02.83 delay 3 seconds @12:13:05.90 Air.reconfigure to mix into 1.37806 parts Lysis1 @12:13:06.34 Air.seek :evac @12:13:06.39 Lysis1.seek 300ul @12:13:21.51 Air.reconfigure :default @12:13:21.57 Air.seek :full @12:13:35.78 Lysis1.seek :empty @12:14:05.86 Lysis Delivered. Syringe pressure @23.9psi @12:14:05.90 PuckHeater.seek 84.4C @12:14:57.76 PuckHeater.hold 15:00,84.4C @12:29:56.88 Lysis complete. Syringe pressure @30.9psi @12:29:56.89 Handoff.select :blocked @12:29:56.99 Lysis1.reconfigure :halfSpeed @12:29:57.07 Lysis1.seek 200ul @12:30:00.85 delay 1 second @12:30:01.88 Lysis1.seek :empty @12:30:05.70 Initial AirS pressure @30.0psi @12:30:05.77 Lysis1.reconfigure :extract @12:30:05.86 Lysis1.seek 900ul,10:00 @12:30:07.58 Air pumping 10 strokes to reach 21psi @12:30:07.59 Air stroke #1 of 10 @12:30:07.61 Air.reconfigure :pressurize @12:30:07.70 Air.seek :pressurized @12:30:20.52 13.2psi after pressurize @12:30:20.53 Air.reconfigure :compress @12:30:20.61 Air.seek :evac @12:30:23.28 16.2psi after compress @12:30:37.51 15.8psi after compress (stable) @12:30:37.53 Air.reconfigure :gulp @12:30:37.61 Air.seek :tVent @12:30:47.49 15.7psi after gulp @12:30:47.50 Air stroke #2 of 10 @12:30:47.52 Air.reconfigure :pressurize @12:30:47.60 Air.seek :pressurized @12:31:00.43 15.5psi after pressurize @12:31:00.46 Air.reconfigure :compress @12:31:00.54 Air.seek :evac @12:31:03.20 16.6psi after compress @12:31:05.97 Air.reconfigure :default @12:31:06.02 Air.hold @12:31:06.22 Lysis1 at 900ul @17.4psi @12:31:06.24 Lysis1.reconfigure :default @12:31:06.32 Lysis1.seek 925ul @12:31:07.04 Syringe::Overpressure in PROCESSING -- 19.5psi after recovery pull -- Skipping SPR @12:31:07.07 SPR.command \"start 2 14\" @12:31:08.49 Handoff.select :waste @12:31:08.97 Ended log /var/log/esp/spr19aug2512h.spr @12:31:08.98 SPR.command \"stats_off\" @12:31:10.93 -> Cmd.stop @12:31:11.02 Handoff.select :close @12:31:11.15 Gate.power :heat,:OFF @12:31:11.17 Gate.power :cartridge,:OFF @12:31:11.25 Carousel.seek 12 @12:31:11.42 Plunge.seek :locked @12:31:12.26 PinPort.seek :clear @12:31:16.88 Archive.seek :clear @12:31:17.01 Air.seek :clear @12:31:17.13 Lysis1.seek :clear @12:31:17.27 Lysis2.seek :clear @12:31:34.63 Archive.coast @12:31:34.68 Air.coast @12:31:34.73 Lysis1.coast @12:31:34.78 Lysis2.coast @12:31:34.83 Plunge.coast @12:31:34.88 SamplePump.coast @12:31:34.93 Toroid.coast @12:31:34.98 Carousel.coast @12:31:35.03 PinPort.coast @12:31:35.21 Gate.power :motor,:OFF @12:31:35.22 Gate.power :core,:OFF @12:31:35.25 Gate.power :analytic1,:OFF @12:31:35.27 Gate.power :analytic2,:OFF @12:31:35.29 Gate.power :analytic3,:OFF @12:31:35.31 Gate.power :main,:OFF @12:31:35.33 Safely stopped and ready to power off ##-- file closed 2019-08-25T18:53:02.523Z ##-- file opened 2019-08-25T18:53:02.527Z @14:53:02.66 -> showStatus @14:53:04.67 -> Cmd.loadCartridge -4 @14:53:04.81 Gate.power :main,:ON,limit to 5000mA @14:53:04.82 Gate.power :analytic1,:ON,limit to 5000mA @14:53:04.84 Gate.power :analytic2,:ON,limit to 5000mA @14:53:04.86 Gate.power :analytic3,:ON,limit to 5000mA @14:53:05.00 Powering core bus @14:53:07.29 SPR Module 1, FW Rev. B, FPGA Rev. C @14:53:07.41 Archive.configure :default @14:53:07.53 Air.configure :default @14:53:07.63 Lysis1.configure :default @14:53:07.73 Lysis2.configure :default @14:53:07.85 Plunge.configure :default @14:53:07.97 SamplePump.configure :default @14:53:08.09 ExtraPump.configure :default @14:53:08.21 Toroid.configure :default @14:53:08.32 Carousel.configure :default @14:53:08.44 Pressure.configure :default @14:53:08.54 PinPort.configure :default @14:53:08.60 All elf controllers running firmware version 4.58 @14:53:08.62 Gate.power :motor,:ON,limit to 5000mA @14:53:08.76 Archive.reconfigure :default @14:53:09.09 Archive.home.jog 0 @14:53:09.22 Toroid.reconfigure :default @14:53:09.30 Plunge.reconfigure :default @14:53:09.34 Lysis1.reconfigure :default @14:53:09.39 PinPort.reconfigure :default @14:53:09.44 Air.reconfigure :default @14:53:09.51 Lysis2.reconfigure :default @14:53:09.74 Air.home.jog 0 @14:53:09.79 PinPort.home.jog 0 @14:53:09.85 Plunge.home.jog 0 @14:53:09.91 Toroid.home.jog 0 @14:53:09.97 Lysis1.home.jog 0 @14:53:10.03 Lysis2.home.jog 0 @14:53:10.31 Archive.reconfigure :homing @14:53:10.47 Archive.jog 1378 @14:53:10.64 Toroid.reconfigure :homing @14:53:10.67 Plunge.reconfigure :homing @14:53:10.71 Air.reconfigure :homing @14:53:10.75 Lysis1.reconfigure :homing @14:53:10.80 PinPort.reconfigure :homing @14:53:10.84 Lysis2.reconfigure :homing @14:53:11.04 Lysis2.jog 1430 @14:53:11.10 Toroid.jog 625 @14:53:11.16 Plunge.jog 1544 @14:53:11.21 Air.jog 1407 @14:53:11.27 Lysis1.jog 1401 @14:53:11.33 PinPort.jog -108 @14:53:11.57 PinPort.setRawPosition! 0 @14:53:11.62 PinPort.seek :home @14:53:11.72 PinPort.reconfigure :default @14:53:11.74 Archive.setRawPosition! 50 @14:53:11.80 Archive.seek :home @14:53:12.11 Archive.reconfigure :default @14:53:12.21 Lysis2.setRawPosition! 50 @14:53:12.25 Lysis2.seek :home @14:53:12.38 Air.setRawPosition! 50 @14:53:12.44 Air.seek :home @14:53:12.55 Lysis1.setRawPosition! 50 @14:53:12.57 Plunge.setRawPosition! 50 @14:53:12.65 Lysis1.seek :home @14:53:12.68 Plunge.seek :home @14:53:13.19 Lysis2.reconfigure :default @14:53:13.23 Air.reconfigure :default @14:53:13.36 Toroid.setRawPosition! 0 @14:53:13.39 Lysis1.reconfigure :default @14:53:13.46 Toroid.seek :home @14:53:13.59 Toroid.reconfigure :default @14:53:13.61 Plunge.reconfigure :default @14:53:13.81 Toroid.seek :clear @14:53:15.43 Carousel.reconfigure :default @14:53:15.50 Carousel.home.jog 0 @14:53:16.00 Carousel.seek 12 @14:53:16.17 Selecting Cartridge 13 @14:53:16.41 Carousel.seek 13 @14:53:16.90 Lysis1.seek :locked @14:53:21.03 Plunge.seek :locked @14:53:21.16 Air.seek :locked @14:53:25.28 Powering cartridge bus @14:53:27.41 Gate.setIR 1750,5000,0,6 @14:53:27.53 Gate.setIR 0,5000,0,6 @14:53:27.57 Loaded Archive Cartridge Gate.power :cartridge,:OFF @14:53:43.15 -> Cmd.startFiltering @14:53:43.38 Duration of filtering limited to 2:05:00 @14:53:43.63 Priming sample loop w/100ml, bypass w/10ml @14:53:43.64 SamplePump.setPosition! 0ml @14:53:43.69 Bypass.open @14:53:45.15 Intake.open @14:53:46.00 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:53:48.83 Vacuum tare is 52% of full scale @14:53:48.96 Pressure.reconfigure :default @14:53:49.02 Pressure.hold @14:53:49.19 SamplePump.reconfigure :prime @14:53:49.26 SamplePump.seek 10ml,1:21 @14:53:58.20 Pressure.coast @14:53:58.25 SamplePump.setPosition! 0ml @14:53:58.30 Exhaust.open @14:53:59.73 Bypass.close @14:54:01.27 Pressure.reconfigure :default @14:54:01.33 Pressure.hold @14:54:01.55 SamplePump.reconfigure :prime @14:54:01.62 SamplePump.seek 100ml,8:51 @14:55:58.80 Pressure.coast @14:55:58.85 Bypass.open @14:55:59.57 Intake.close @14:56:00.40 Toroid.reconfigure :hold @14:56:00.47 Toroid.seek :crack @14:56:01.26 Toroid.reconfigure :default @14:56:01.32 Toroid.seek :clear @14:56:02.88 Toroid.reconfigure :hold @14:56:02.95 Toroid.seek :cartridge @14:56:04.77 Toroid.reconfigure :default @14:56:04.83 Toroid.seek :clear @14:56:06.81 Pressurizing cartridge to 26psi above 13.4psia @14:56:06.82 SamplePump.setPosition! 0ml @14:56:06.87 Intake.open @14:56:07.58 Bypass.close @14:56:08.28 Exhaust.close @14:56:09.10 Pressure.reconfigure :default @14:56:09.15 Pressure.hold @14:56:09.32 SamplePump.reconfigure :charge @14:56:09.40 SamplePump.seek 2.3ml,42 seconds @14:56:17.86 Pressure.coast @14:56:17.91 Intake.close @14:56:18.74 Pressurized cartridge with 0.334ml to 26.0psi @14:56:18.75 Isolated @35.7psia (22.4psi) @14:56:18.76 Checking for cartridge leaks from 35.7psia @14:56:18.79 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @14:56:23.84 Cartridge pressure fell 1% to 35.4psia @14:56:23.92 Sampling 1000.0ml @14:56:23.93 SamplePump.setPosition! 0ml @14:56:23.98 Exhaust.open @14:56:24.69 Intake.open @14:56:25.54 Pressure.reconfigure :default @14:56:25.60 Pressure.hold @14:56:25.82 SamplePump.reconfigure :sample @14:56:25.89 SamplePump.seek 1000ml,1:23:53 @14:56:28.58 Cartridge 13 sampling 1/1000ml at 0.37ml/s with 22.1psi vacuum @14:57:28.96 Cartridge 13 sampling 21/1000ml at 0.27ml/s with 26.2psi vacuum @14:58:28.58 Cartridge 13 sampling 36/1000ml at 0.20ml/s with 22.7psi vacuum @14:59:00.10 Flow averaged 0.28ml/s over 2:33 @14:59:00.13 Waiting up to 12 seconds for vacuum to reduce from 27.4 to 18.0psi @14:59:01.13 Relieved excess vacuum in 2.4 seconds @14:59:01.15 SamplePump.seek 1000ml,1:20:21 @14:59:05.92 Flow estimate reduced to 0.22ml/s @14:59:05.95 Waiting up to 12 seconds for vacuum to reduce from 27.7 to 18.0psi @14:59:06.95 Relieved excess vacuum in 2.7 seconds @14:59:06.97 SamplePump.seek 1000ml,1:20:16 @14:59:11.59 Flow estimate reduced to 0.18ml/s @14:59:11.62 Intake.close @14:59:11.67 Exhaust.close @14:59:11.85 Toroid.reconfigure :hold @14:59:11.92 Toroid.seek :bypass @14:59:13.54 Toroid.reconfigure :default @14:59:13.61 Toroid.seek :clear @14:59:15.23 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @14:59:15.25 Pressure.coast @14:59:15.43 Sampled 44.2ml @14:59:17.65 -> Cmd.startProcessing @14:59:17.90 Plunge.seek :reagent @14:59:18.76 Sample loop closed. Ready to Deliver Reagents @14:59:18.77 Archive.seek :sealed @14:59:23.05 Archive.seek :evac @14:59:36.99 delay 1:00 @15:00:37.02 Archive.seek :clear @15:00:54.74 RNAlater soak for 10:00 @15:10:54.77 Archive.seek :tVent @15:10:57.35 delay 1 second @15:10:58.37 Archive.seek :evac @15:11:13.99 delay 10 seconds @15:11:24.02 Archive.seek :tVent @15:11:39.64 delay 1 second @15:11:40.66 Archive.seek :evac @15:11:56.29 delay 10 seconds @15:12:06.32 Sample preserved and evacuated @15:12:07.41 -> Cmd.stop @15:12:07.55 Carousel.seek 13 @15:12:07.72 Plunge.seek :locked @15:12:08.56 PinPort.seek :clear @15:12:08.70 Archive.seek :clear @15:12:08.82 Air.seek :clear @15:12:08.95 Lysis1.seek :clear @15:12:26.46 Archive.coast @15:12:26.50 Air.coast @15:12:26.55 Lysis1.coast @15:12:26.60 Lysis2.coast @15:12:26.65 Plunge.coast @15:12:26.70 SamplePump.coast @15:12:26.76 Toroid.coast @15:12:26.80 Carousel.coast @15:12:26.86 PinPort.coast @15:12:27.04 Gate.power :motor,:OFF @15:12:27.05 Gate.power :core,:OFF @15:12:27.08 Gate.power :analytic1,:OFF @15:12:27.10 Gate.power :analytic2,:OFF @15:12:27.12 Gate.power :analytic3,:OFF @15:12:27.14 Gate.power :main,:OFF @15:12:27.16 Safely stopped and ready to power off ##-- file closed 2019-08-25T19:28:16.119Z ##-- file opened 2019-08-25T19:28:16.120Z @15:28:16.46 -> showStatus @15:28:18.48 -> Cmd.loadCartridge -15 @15:28:18.62 Gate.power :main,:ON,limit to 5000mA @15:28:18.63 Gate.power :analytic1,:ON,limit to 5000mA @15:28:18.66 Gate.power :analytic2,:ON,limit to 5000mA @15:28:18.68 Gate.power :analytic3,:ON,limit to 5000mA @15:28:18.81 Powering core bus @15:28:21.09 SPR Module 1, FW Rev. B, FPGA Rev. C @15:28:21.22 Archive.configure :default @15:28:21.34 Air.configure :default @15:28:21.44 Lysis1.configure :default @15:28:21.54 Lysis2.configure :default @15:28:21.66 Plunge.configure :default @15:28:21.78 SamplePump.configure :default @15:28:21.90 ExtraPump.configure :default @15:28:22.06 Toroid.configure :default @15:28:22.18 Carousel.configure :default @15:28:22.30 Pressure.configure :default @15:28:22.40 PinPort.configure :default @15:28:22.46 All elf controllers running firmware version 4.58 @15:28:22.48 Gate.power :motor,:ON,limit to 5000mA @15:28:22.70 Archive.reconfigure :default @15:28:22.94 Archive.home.jog 0 @15:28:23.07 Toroid.reconfigure :default @15:28:23.15 Plunge.reconfigure :default @15:28:23.18 Lysis1.reconfigure :default @15:28:23.22 Air.reconfigure :default @15:28:23.26 PinPort.reconfigure :default @15:28:23.33 Lysis2.reconfigure :default @15:28:23.55 Lysis2.home.jog 0 @15:28:23.60 Lysis1.home.jog 0 @15:28:23.66 Toroid.home.jog 0 @15:28:23.71 Plunge.home.jog 0 @15:28:23.76 Air.home.jog 0 @15:28:23.82 PinPort.home.jog 0 @15:28:24.16 Archive.reconfigure :homing @15:28:24.37 Toroid.reconfigure :homing @15:28:24.40 Plunge.reconfigure :homing @15:28:24.43 Air.reconfigure :homing @15:28:24.47 PinPort.reconfigure :homing @15:28:24.50 Lysis1.reconfigure :homing @15:28:24.54 Lysis2.reconfigure :homing @15:28:24.77 Lysis1.jog 1404 @15:28:24.82 Toroid.jog 630 @15:28:24.88 Plunge.jog 1539 @15:28:24.93 Air.jog 1408 @15:28:24.99 Lysis2.jog 1430 @15:28:25.05 Archive.jog 1377 @15:28:25.12 PinPort.jog -107 @15:28:25.34 PinPort.setRawPosition! 0 @15:28:25.38 PinPort.seek :home @15:28:25.47 PinPort.reconfigure :default @15:28:25.93 Lysis1.setRawPosition! 50 @15:28:25.97 Lysis1.seek :home @15:28:26.10 Plunge.setRawPosition! 50 @15:28:26.16 Plunge.seek :home @15:28:26.27 Lysis2.setRawPosition! 50 @15:28:26.30 Lysis1.reconfigure :default @15:28:26.32 Air.setRawPosition! 50 @15:28:26.34 Archive.setRawPosition! 50 @15:28:26.49 Lysis2.seek :home @15:28:26.54 Air.seek :home @15:28:26.57 Archive.seek :home @15:28:27.14 Toroid.setRawPosition! 0 @15:28:27.16 Plunge.reconfigure :default @15:28:27.25 Toroid.seek :home @15:28:27.39 Toroid.reconfigure :default @15:28:27.43 Lysis2.reconfigure :default @15:28:27.46 Air.reconfigure :default @15:28:27.49 Archive.reconfigure :default @15:28:27.98 Toroid.seek :clear @15:28:29.60 Carousel.reconfigure :default @15:28:29.67 Carousel.home.jog 0 @15:28:30.16 Carousel.seek 13 @15:28:30.30 Selecting Cartridge 10 @15:28:30.54 Carousel.seek 10 @15:28:31.43 Lysis1.seek :locked @15:28:35.58 Plunge.seek :locked @15:28:35.70 Air.seek :locked @15:28:39.83 Powering cartridge bus @15:28:41.96 Gate.setIR 1750,5000,0,6 @15:28:42.08 Gate.setIR 0,5000,0,6 @15:28:42.14 Loaded Lyse-n-Go Cartridge @15:28:42.15 PuckHeater.configure HeatConfig @15:28:42.20 Gate.power :heat,:ON,limit to 5000mA @15:28:57.71 -> Cmd.startFiltering @15:28:57.93 Duration of filtering limited to 2:05:00 @15:28:58.19 Priming sample loop w/100ml, bypass w/10ml @15:28:58.20 SamplePump.setPosition! 0ml @15:28:58.24 Bypass.open @15:28:59.73 Intake.open @15:29:00.57 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:29:03.40 Vacuum tare is 52% of full scale @15:29:03.53 Pressure.reconfigure :default @15:29:03.59 Pressure.hold @15:29:03.75 SamplePump.reconfigure :prime @15:29:03.82 SamplePump.seek 10ml,1:21 @15:29:12.78 Pressure.coast @15:29:12.83 SamplePump.setPosition! 0ml @15:29:12.88 Exhaust.open @15:29:14.34 Bypass.close @15:29:15.90 Pressure.reconfigure :default @15:29:15.96 Pressure.hold @15:29:16.17 SamplePump.reconfigure :prime @15:29:16.25 SamplePump.seek 100ml,8:51 @15:31:13.13 Pressure.coast @15:31:13.19 Bypass.open @15:31:13.90 Intake.close @15:31:14.76 Toroid.reconfigure :hold @15:31:14.82 Toroid.seek :crack @15:31:15.62 Toroid.reconfigure :default @15:31:15.68 Toroid.seek :clear @15:31:17.24 Toroid.reconfigure :hold @15:31:17.31 Toroid.seek :cartridge @15:31:19.12 Toroid.reconfigure :default @15:31:19.18 Toroid.seek :clear @15:31:21.15 Pressurizing cartridge to 26psi above 12.7psia @15:31:21.16 SamplePump.setPosition! 0ml @15:31:21.20 Intake.open @15:31:21.96 Bypass.close @15:31:22.67 Exhaust.close @15:31:23.48 Pressure.reconfigure :default @15:31:23.53 Pressure.hold @15:31:23.70 SamplePump.reconfigure :charge @15:31:23.77 SamplePump.seek 2.3ml,42 seconds @15:31:35.46 Pressure.coast @15:31:35.51 Intake.close @15:31:36.34 Pressurized cartridge with 0.641ml to 26.2psi @15:31:36.35 Isolated @36.1psia (23.4psi) @15:31:36.36 Checking for cartridge leaks from 36.1psia @15:31:36.39 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @15:31:41.48 Sampling 1000.0ml @15:31:41.50 SamplePump.setPosition! 0ml @15:31:41.55 Exhaust.open @15:31:42.25 Intake.open @15:31:43.12 Pressure.reconfigure :default @15:31:43.17 Pressure.hold @15:31:43.39 SamplePump.reconfigure :sample @15:31:43.47 SamplePump.seek 1000ml,1:23:53 @15:31:46.16 Cartridge 10 sampling 1/1000ml at 0.53ml/s with 25.5psi vacuum @15:32:46.17 Cartridge 10 sampling 32/1000ml at 0.50ml/s with 23.0psi vacuum @15:33:46.15 Cartridge 10 sampling 61/1000ml at 0.47ml/s with 23.6psi vacuum @15:34:46.16 Cartridge 10 sampling 88/1000ml at 0.41ml/s with 23.7psi vacuum @15:35:46.16 Cartridge 10 sampling 112/1000ml at 0.38ml/s with 23.5psi vacuum @15:36:46.16 Cartridge 10 sampling 134/1000ml at 0.36ml/s with 26.5psi vacuum @15:37:46.16 Cartridge 10 sampling 154/1000ml at 0.31ml/s with 22.6psi vacuum @15:38:46.15 Cartridge 10 sampling 172/1000ml at 0.28ml/s with 23.0psi vacuum @15:39:46.16 Cartridge 10 sampling 189/1000ml at 0.23ml/s with 26.0psi vacuum @15:40:46.16 Cartridge 10 sampling 203/1000ml at 0.22ml/s with 21.5psi vacuum @15:41:46.16 Cartridge 10 sampling 215/1000ml at 0.20ml/s with 25.1psi vacuum @15:42:46.16 Cartridge 10 sampling 227/1000ml at 0.20ml/s with 27.3psi vacuum @15:43:10.59 Flow averaged 0.34ml/s over 11:23 @15:43:10.62 Waiting up to 14 seconds for vacuum to reduce from 28.6 to 18.0psi @15:43:11.63 Relieved excess vacuum in 5.4 seconds @15:43:11.65 SamplePump.seek 1000ml,1:04:30 @15:43:35.63 Flow averaged 0.21ml/s over 24 seconds @15:43:35.66 Waiting up to 15 seconds for vacuum to reduce from 28.8 to 18.0psi @15:43:36.66 Relieved excess vacuum in 1.9 seconds @15:43:36.68 SamplePump.seek 1000ml,1:04:06 @15:43:38.44 Flow estimate reduced to 0.17ml/s @15:43:38.46 Intake.close @15:43:38.52 Exhaust.close @15:43:38.69 Toroid.reconfigure :hold @15:43:38.76 Toroid.seek :bypass @15:43:40.37 Toroid.reconfigure :default @15:43:40.44 Toroid.seek :clear @15:43:42.06 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @15:43:42.08 Pressure.coast @15:43:42.26 Sampled 237.5ml @15:43:44.61 -> Cmd.startProcessing @15:43:46.47 SPR.command \"read_sequences\" @15:43:46.96 SPR.command \"stats_on\" @15:43:47.00 SPR.command \"start 0 1\" @15:43:49.00 Begun log /var/log/esp/spr19aug2515h.spr @15:43:49.80 Handoff.select :waste @15:43:49.98 Archive.seek :empty @15:43:57.15 Syringe tare is 21% of full scale @15:44:07.69 PinPort.seek :cartridge @15:44:12.25 Lysis1.seek 1350ul @15:44:14.72 Lysis2.seek 1700ul @15:44:17.81 Plunge.seek :reagent @15:44:18.66 Evacuating puck with filter bubble point of 50psi @15:44:18.68 PinPort.seek :cartridge @15:44:18.76 Handoff.select :waste @15:44:33.09 Air pumping 1 strokes to reach 8psi @15:44:33.11 Air.reconfigure :gulp @15:44:33.18 Air.seek :tVent @15:44:34.97 0.6psi after gulp @15:44:34.98 Air stroke #1 of 1 @15:44:35.00 Air.reconfigure :pressurize @15:44:35.08 Air.seek :pressurized @15:44:47.91 0.4psi after pressurize @15:44:47.92 Air.reconfigure :compress @15:44:48.01 Air.seek :evac @15:44:50.68 1.2psi after compress @15:45:04.97 0.4psi after compress (stable) @15:45:05.13 at 0.4psi Opened to waste @15:45:05.14 Handoff.select :blocked @15:45:05.32 Air pumping 10 strokes to reach 50psi @15:45:05.33 Air.reconfigure :gulp @15:45:05.41 Air.seek :tVent @15:45:15.31 10.3psi after gulp @15:45:15.32 Air stroke #1 of 10 @15:45:15.34 Air.reconfigure :pressurize @15:45:15.44 Air.seek :pressurized @15:45:28.27 13.9psi after pressurize @15:45:28.28 Air.reconfigure :compress @15:45:28.37 Air.seek :evac @15:45:31.03 16.4psi after compress @15:46:03.20 38.6psi after compress (stable) @15:46:03.23 Air.reconfigure :gulp @15:46:03.31 Air.seek :tVent @15:46:13.19 40.4psi after gulp @15:46:13.20 Air stroke #2 of 10 @15:46:13.22 Air.reconfigure :pressurize @15:46:13.30 Air.seek :pressurized @15:46:26.14 37.4psi after pressurize @15:46:26.15 Air.reconfigure :compress @15:46:26.23 Air.seek :evac @15:46:28.89 36.6psi after compress @15:46:36.62 Reached 50.1 of 50.0psi after pumping 4672ul (3.00 strokes) @15:46:36.63 Handoff.select :waste @15:46:58.73 delay 30 seconds @15:47:28.75 Handoff.select :blocked @15:47:45.57 Pressure too High, open to waste again (8.1psi) @15:47:45.59 Handoff.select :waste @15:47:45.71 delay 30 seconds @15:48:15.73 Handoff.select :blocked @15:48:40.72 Handoff.select :waste @15:48:40.84 PSI at @2.52psi at evacEnd @15:48:40.85 Handoff.select :blocked @15:48:40.97 Air.reconfigure :gulp @15:48:41.08 Air.seek :tVent @15:48:58.18 Stable @2.0psi after Handoff blocked @15:48:58.26 Air pumping 10 strokes to reach 32psi @15:48:58.28 Air stroke #1 of 10 @15:48:58.30 Air.reconfigure :pressurize @15:48:58.38 Air.seek :pressurized @15:49:11.20 2.4psi after pressurize @15:49:11.21 Air.reconfigure :compress @15:49:11.29 Air.seek :evac @15:49:14.34 6.1psi after compress @15:49:38.20 14.5psi after compress (stable) @15:49:38.22 Air.reconfigure :gulp @15:49:38.30 Air.seek :tVent @15:49:48.19 14.8psi after gulp @15:49:48.20 Air stroke #2 of 10 @15:49:48.22 Air.reconfigure :pressurize @15:49:48.30 Air.seek :pressurized @15:50:01.14 14.8psi after pressurize @15:50:01.15 Air.reconfigure :compress @15:50:01.23 Air.seek :evac @15:50:03.90 18.2psi after compress @15:50:22.77 25.8psi after compress (stable) @15:50:22.81 Air.reconfigure :gulp @15:50:22.90 Air.seek :tVent @15:50:32.79 26.1psi after gulp @15:50:32.80 Air stroke #3 of 10 @15:50:32.82 Air.reconfigure :pressurize @15:50:32.90 Air.seek :pressurized @15:50:45.73 26.0psi after pressurize @15:50:45.74 Air.reconfigure :compress @15:50:45.82 Air.seek :evac @15:50:48.49 28.4psi after compress @15:50:50.33 Reached 32.1 of 32.0psi after pumping 6230ul (4.00 strokes) @15:50:50.35 Handoff.select :waste @15:51:02.74 delay 30 seconds @15:51:32.76 Handoff.select :blocked @15:51:57.81 Handoff.select :waste @15:51:57.98 PSI at @0.69psi at evacEnd @15:51:57.99 Air.reconfigure :default @15:51:58.11 Air.seek :fVent @15:51:59.55 delay 5 seconds @15:52:04.57 Seawater evacuated, Presure above/below filter released. Lysis begins @15:52:04.65 Lysis1.reconfigure :slow @15:52:04.71 Lysis1.seek 1050ul @15:52:34.73 delay 5 seconds @15:52:39.75 Handoff.select :blocked @15:52:39.91 Lysis1.seek 450ul @15:53:39.87 delay 3 seconds @15:53:42.94 Air.reconfigure to mix into 1.37806 parts Lysis1 @15:53:43.44 Air.seek :evac @15:53:43.48 Lysis1.seek 300ul @15:53:58.58 Air.reconfigure :default @15:53:58.64 Air.seek :full @15:54:12.85 Lysis1.seek :empty @15:54:42.96 Lysis Delivered. Syringe pressure @37.6psi @15:54:43.01 PuckHeater.seek 84.4C @15:55:30.46 PuckHeater.hold 15:00,84.4C @16:10:29.10 Lysis complete. Syringe pressure @46.5psi @16:10:29.11 Handoff.select :blocked @16:10:29.21 Lysis1.reconfigure :halfSpeed @16:10:29.28 Lysis1.seek 200ul @16:10:33.04 delay 1 second @16:10:34.06 Lysis1.seek :empty @16:10:37.88 Initial AirS pressure @46.9psi @16:10:37.94 Lysis1.reconfigure :extract @16:10:38.03 Lysis1.seek 900ul,10:00 @16:10:39.19 Air pumping 10 strokes to reach 21psi @16:10:39.21 Air stroke #1 of 10 @16:10:39.23 Air.reconfigure :pressurize @16:10:39.31 Air.seek :pressurized @16:10:52.14 15.9psi after pressurize @16:10:52.15 Air.reconfigure :compress @16:10:52.23 Air.seek :evac @16:10:54.89 16.8psi after compress @16:11:09.20 15.8psi after compress (stable) @16:11:09.22 Air.reconfigure :gulp @16:11:09.30 Air.seek :tVent @16:11:19.18 15.2psi after gulp @16:11:19.19 Air stroke #2 of 10 @16:11:19.21 Air.reconfigure :pressurize @16:11:19.29 Air.seek :pressurized @16:11:32.13 15.4psi after pressurize @16:11:32.14 Air.reconfigure :compress @16:11:32.22 Air.seek :evac @16:11:34.88 15.9psi after compress @16:11:48.80 15.6psi after compress (stable) @16:11:48.82 Air.reconfigure :gulp @16:11:48.90 Air.seek :tVent @16:11:58.78 15.8psi after gulp @16:11:58.79 Air stroke #3 of 10 @16:11:58.81 Air.reconfigure :pressurize @16:11:58.90 Air.seek :pressurized @16:12:11.72 12.3psi after pressurize @16:12:11.73 Air.reconfigure :compress @16:12:11.82 Air.seek :evac @16:12:14.50 16.6psi after compress @16:12:17.13 Air.reconfigure :default @16:12:17.18 Air.hold @16:12:17.35 Lysis1 at 900ul @15.8psi @16:12:17.36 Lysis1.reconfigure :default @16:12:17.43 Lysis1.seek 925ul @16:12:18.21 Lysis1 at 925ul, bottom @13.7psi after recovery @16:12:19.32 Lysis1 at 925ul, bottom @19.2psi @16:12:19.34 Air.reconfigure :halfSpeed @16:12:19.43 Air.seek :fVent @16:12:21.68 Puck Top Vented, bottom @1.3psi @16:12:21.69 Handoff.select :waste @16:12:21.81 delay 5 seconds @16:12:26.83 delay 5 seconds @16:12:31.85 Handoff.select SPR @16:12:31.96 SPR.command \"start 2 14\" @16:12:33.21 delay 10 seconds @16:12:43.23 SPR.command \"start 2 0\" @16:12:46.96 Waiting up to 11:00 for SPR to enter state 1 @16:21:19.47 SPR.command \"hold_on\" @16:21:20.88 SPR Dilution 1 of 1 -- Running Experiment 2 @16:21:20.89 Lysis2.reconfigure :DAmix @16:21:21.01 Lysis1.reconfigure :DAmix @16:21:21.40 Lysis2.seek 1450ul @16:21:21.49 Lysis1.seek 675ul @16:21:56.83 Lysis2.seek 1150ul @16:21:56.86 Lysis1.seek 375ul @16:22:38.86 volL1 = 300.0 and volL2 = 300.0 @16:22:38.88 delay 15 seconds @16:22:53.92 SPR.command \"hold_off\" @16:22:56.53 Waiting up to 25:00 for SPR to enter state 7 @16:34:33.04 SPR Analysis has Completed @16:34:33.05 Handoff.select :waste @16:34:33.96 Ended log /var/log/esp/spr19aug2515h.spr @16:34:33.97 SPR.command \"stats_off\" @16:34:35.39 -> Cmd.stop @16:34:35.48 Handoff.select :close @16:34:35.61 Gate.power :heat,:OFF @16:34:35.63 Gate.power :cartridge,:OFF @16:34:35.71 Carousel.seek 10 @16:34:35.89 Plunge.seek :locked @16:34:36.72 PinPort.seek :clear @16:34:41.33 Archive.seek :clear @16:34:41.46 Air.reconfigure :default @16:34:41.58 Lysis1.reconfigure :default @16:34:41.70 Air.seek :clear @16:34:41.76 Lysis1.seek :clear @16:34:41.84 Lysis2.reconfigure :default @16:34:41.91 Lysis2.seek :clear @16:34:59.07 Archive.coast @16:34:59.12 Air.coast @16:34:59.17 Lysis1.coast @16:34:59.22 Lysis2.coast @16:34:59.27 Plunge.coast @16:34:59.32 SamplePump.coast @16:34:59.37 Toroid.coast @16:34:59.43 Carousel.coast @16:34:59.48 PinPort.coast @16:34:59.66 Gate.power :motor,:OFF @16:34:59.67 Gate.power :core,:OFF @16:34:59.70 Gate.power :analytic1,:OFF @16:34:59.72 Gate.power :analytic2,:OFF @16:34:59.73 Gate.power :analytic3,:OFF @16:34:59.75 Gate.power :main,:OFF @16:34:59.77 Safely stopped and ready to power off ##-- file closed 2019-08-26T22:12:27.993Z