##-- file opened 2019-08-19T21:55:34.086Z @17:55:34.40 -> showStatus @17:55:36.02 -> Cmd.loadCartridge -4 @17:55:36.17 Gate.power :main,:ON,limit to 5000mA @17:55:36.18 Gate.power :analytic1,:ON,limit to 5000mA @17:55:36.20 Gate.power :analytic2,:ON,limit to 5000mA @17:55:36.22 Gate.power :analytic3,:ON,limit to 5000mA @17:55:36.36 Powering core bus @17:55:38.64 SPR Module 1, FW Rev. B, FPGA Rev. C @17:55:38.77 Archive.configure :default @17:55:38.89 Air.configure :default @17:55:39.07 Lysis1.configure :default @17:55:39.17 Lysis2.configure :default @17:55:39.29 Plunge.configure :default @17:55:39.40 SamplePump.configure :default @17:55:39.52 ExtraPump.configure :default @17:55:39.64 Toroid.configure :default @17:55:39.76 Carousel.configure :default @17:55:39.88 Pressure.configure :default @17:55:39.98 PinPort.configure :default @17:55:40.04 All elf controllers running firmware version 4.58 @17:55:40.06 Gate.power :motor,:ON,limit to 5000mA @17:55:40.27 Air.reconfigure :default @17:55:40.31 Archive.reconfigure :default @17:55:40.59 Air.home.jog 0 @17:55:40.65 Archive.home.jog 0 @17:55:40.78 Toroid.reconfigure :default @17:55:40.86 Plunge.reconfigure :default @17:55:40.89 Lysis1.reconfigure :default @17:55:40.95 PinPort.reconfigure :default @17:55:41.01 Lysis2.reconfigure :default @17:55:41.24 Lysis2.home.jog 0 @17:55:41.29 Plunge.home.jog 0 @17:55:41.35 PinPort.home.jog 0 @17:55:41.41 Toroid.home.jog 0 @17:55:41.47 Lysis1.home.jog 0 @17:55:41.79 Air.reconfigure :homing @17:55:41.82 Archive.reconfigure :homing @17:55:41.93 Air.jog 1411 @17:55:41.99 Archive.jog 1383 @17:55:42.14 PinPort.reconfigure :homing @17:55:42.17 Toroid.reconfigure :homing @17:55:42.21 Lysis1.reconfigure :homing @17:55:42.24 Lysis2.reconfigure :homing @17:55:42.29 Plunge.reconfigure :homing @17:55:42.48 Lysis2.jog 1427 @17:55:42.53 Lysis1.jog 1399 @17:55:42.60 Plunge.jog 1548 @17:55:42.66 PinPort.jog -108 @17:55:42.71 Toroid.jog 634 @17:55:42.89 PinPort.setRawPosition! 0 @17:55:42.93 PinPort.seek :home @17:55:43.02 PinPort.reconfigure :default @17:55:43.10 Air.setRawPosition! 50 @17:55:43.16 Air.seek :home @17:55:43.24 Archive.setRawPosition! 50 @17:55:43.29 Archive.seek :home @17:55:43.44 Air.reconfigure :default @17:55:43.57 Archive.reconfigure :default @17:55:43.65 Lysis2.setRawPosition! 50 @17:55:43.71 Lysis2.seek :home @17:55:43.80 Lysis1.setRawPosition! 50 @17:55:43.84 Lysis1.seek :home @17:55:43.93 Plunge.setRawPosition! 50 @17:55:43.99 Plunge.seek :home @17:55:44.09 Lysis2.reconfigure :default @17:55:44.17 Lysis1.reconfigure :default @17:55:44.28 Plunge.reconfigure :default @17:55:44.87 Toroid.setRawPosition! 0 @17:55:44.92 Toroid.seek :home @17:55:45.00 Toroid.reconfigure :default @17:55:45.14 Toroid.seek :clear @17:55:46.76 Carousel.reconfigure :default @17:55:46.84 Carousel.home.jog 0 @17:55:47.33 Carousel.seek 46 @17:55:47.56 Selecting Cartridge 47 @17:55:47.80 Carousel.seek 47 @17:55:48.28 Lysis1.seek :locked @17:55:52.42 Plunge.seek :locked @17:55:52.54 Air.seek :locked @17:55:56.67 Powering cartridge bus @17:55:58.80 Gate.setIR 1750,5000,0,6 @17:55:58.93 Gate.setIR 0,5000,0,6 @17:55:58.97 Loaded Archive Cartridge Gate.power :cartridge,:OFF @17:56:14.41 -> Cmd.startFiltering @17:56:14.63 Duration of filtering limited to 2:05:00 @17:56:15.25 Priming sample loop w/100ml, bypass w/10ml @17:56:15.27 SamplePump.setPosition! 0ml @17:56:15.32 Bypass.open @17:56:16.79 Intake.open @17:56:17.63 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:56:20.45 Vacuum tare is 52% of full scale @17:56:20.58 Pressure.reconfigure :default @17:56:20.64 Pressure.hold @17:56:20.81 SamplePump.reconfigure :prime @17:56:20.88 SamplePump.seek 10ml,1:21 @17:56:29.84 Pressure.coast @17:56:29.90 SamplePump.setPosition! 0ml @17:56:29.94 Exhaust.open @17:56:31.38 Bypass.close @17:56:32.90 Pressure.reconfigure :default @17:56:32.96 Pressure.hold @17:56:33.18 SamplePump.reconfigure :prime @17:56:33.25 SamplePump.seek 100ml,8:51 @17:58:24.48 Pressure.coast @17:58:24.53 Bypass.open @17:58:25.25 Intake.close @17:58:26.10 Toroid.reconfigure :hold @17:58:26.16 Toroid.seek :crack @17:58:26.96 Toroid.reconfigure :default @17:58:27.02 Toroid.seek :clear @17:58:28.58 Toroid.reconfigure :hold @17:58:28.65 Toroid.seek :cartridge @17:58:30.46 Toroid.reconfigure :default @17:58:30.52 Toroid.seek :clear @17:58:32.49 Pressurizing cartridge to 26psi above 15.5psia @17:58:32.50 SamplePump.setPosition! 0ml @17:58:32.54 Intake.open @17:58:33.30 Bypass.close @17:58:34.04 Exhaust.close @17:58:34.87 Pressure.reconfigure :default @17:58:34.93 Pressure.hold @17:58:35.09 SamplePump.reconfigure :charge @17:58:35.16 SamplePump.seek 2.3ml,42 seconds @17:58:42.90 Pressure.coast @17:58:42.95 Intake.close @17:58:43.79 Pressurized cartridge with 0.278ml to 26.2psi @17:58:43.80 Isolated @38.3psia (22.9psi) @17:58:43.81 Checking for cartridge leaks from 38.3psia @17:58:43.85 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @17:58:48.89 Cartridge pressure fell 1% to 38.1psia @17:58:48.97 Sampling 1000.0ml @17:58:48.98 SamplePump.setPosition! 0ml @17:58:49.03 Exhaust.open @17:58:49.76 Intake.open @17:58:50.62 Pressure.reconfigure :default @17:58:50.68 Pressure.hold @17:58:50.90 SamplePump.reconfigure :sample @17:58:50.98 SamplePump.seek 1000ml,1:23:53 @17:58:53.65 Cartridge 47 sampling 1/1000ml at 0.26ml/s with 23.1psi vacuum @17:59:53.66 Cartridge 47 sampling 15/1000ml at 0.26ml/s with 24.0psi vacuum @18:00:53.65 Cartridge 47 sampling 29/1000ml at 0.27ml/s with 24.4psi vacuum @18:01:53.66 Cartridge 47 sampling 45/1000ml at 0.33ml/s with 24.1psi vacuum @18:02:53.66 Cartridge 47 sampling 64/1000ml at 0.30ml/s with 26.1psi vacuum @18:03:53.66 Cartridge 47 sampling 84/1000ml at 0.31ml/s with 20.8psi vacuum @18:04:53.66 Cartridge 47 sampling 104/1000ml at 0.31ml/s with 22.6psi vacuum @18:05:53.66 Cartridge 47 sampling 123/1000ml at 0.32ml/s with 21.8psi vacuum @18:06:53.66 Cartridge 47 sampling 142/1000ml at 0.31ml/s with 23.4psi vacuum @18:07:53.66 Cartridge 47 sampling 159/1000ml at 0.32ml/s with 25.1psi vacuum @18:08:53.66 Cartridge 47 sampling 176/1000ml at 0.24ml/s with 25.9psi vacuum @18:09:53.66 Cartridge 47 sampling 193/1000ml at 0.23ml/s with 23.4psi vacuum @18:10:53.66 Cartridge 47 sampling 208/1000ml at 0.24ml/s with 28.2psi vacuum @18:11:53.66 Cartridge 47 sampling 223/1000ml at 0.25ml/s with 20.5psi vacuum @18:12:53.66 Cartridge 47 sampling 237/1000ml at 0.24ml/s with 22.9psi vacuum @18:13:53.66 Cartridge 47 sampling 251/1000ml at 0.20ml/s with 23.8psi vacuum @18:14:53.66 Cartridge 47 sampling 264/1000ml at 0.22ml/s with 25.6psi vacuum @18:15:53.66 Cartridge 47 sampling 277/1000ml at 0.21ml/s with 25.0psi vacuum @18:16:53.66 Cartridge 47 sampling 289/1000ml at 0.20ml/s with 26.9psi vacuum @18:17:53.66 Cartridge 47 sampling 301/1000ml at 0.20ml/s with 26.9psi vacuum @18:18:53.66 Cartridge 47 sampling 314/1000ml at 0.20ml/s with 27.3psi vacuum @18:19:34.85 Flow averaged 0.26ml/s over 20:43 @18:19:34.88 Waiting up to 14 seconds for vacuum to reduce from 28.6 to 18.0psi @18:19:35.96 Relieved excess vacuum in 2.1 seconds @18:19:35.98 SamplePump.seek 1000ml,57:02 @18:19:53.66 Cartridge 47 sampling 325/1000ml at 0.20ml/s with 23.3psi vacuum @18:20:06.18 Flow averaged 0.22ml/s over 28 seconds @18:20:06.21 Waiting up to 14 seconds for vacuum to reduce from 28.3 to 18.0psi @18:20:06.91 Relieved excess vacuum in 3.2 seconds @18:20:06.93 SamplePump.seek 1000ml,56:32 @18:20:27.64 Flow averaged 0.32ml/s over 14 seconds @18:20:27.67 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi @18:20:28.37 Relieved excess vacuum in 8.3 seconds @18:20:28.39 SamplePump.seek 1000ml,56:11 @18:20:38.06 Flow estimate reduced to 0.25ml/s @18:20:38.10 Waiting up to 14 seconds for vacuum to reduce from 28.2 to 18.0psi @18:20:38.80 Relieved excess vacuum in 7.3 seconds @18:20:38.82 SamplePump.seek 1000ml,56:02 @18:20:53.65 Cartridge 47 sampling 337/1000ml at 0.20ml/s with 26.4psi vacuum @18:21:07.59 Flow averaged 0.23ml/s over 25 seconds @18:21:07.62 Waiting up to 14 seconds for vacuum to reduce from 28.3 to 18.0psi @18:21:08.32 Relieved excess vacuum in 4.6 seconds @18:21:08.34 SamplePump.seek 1000ml,55:33 @18:21:16.51 Flow estimate reduced to 0.18ml/s @18:21:16.53 Intake.close @18:21:16.57 Exhaust.close @18:21:16.74 Toroid.reconfigure :hold @18:21:16.82 Toroid.seek :bypass @18:21:18.43 Toroid.reconfigure :default @18:21:18.50 Toroid.seek :clear @18:21:20.12 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @18:21:20.14 Pressure.coast @18:21:20.32 Sampled 341.4ml @18:21:22.28 -> Cmd.startProcessing @18:21:22.53 Plunge.seek :reagent @18:21:23.39 Sample loop closed. Ready to Deliver Reagents @18:21:23.40 Archive.seek :sealed @18:21:27.67 Archive.seek :evac @18:21:41.60 delay 1:00 @18:22:41.63 Archive.seek :clear @18:22:59.35 RNAlater soak for 10:00 @18:32:59.37 Archive.seek :tVent @18:33:01.95 delay 1 second @18:33:02.97 Archive.seek :evac @18:33:18.59 delay 10 seconds @18:33:28.62 Archive.seek :tVent @18:33:44.24 delay 1 second @18:33:45.26 Archive.seek :evac @18:34:00.89 delay 10 seconds @18:34:10.92 Sample preserved and evacuated @18:34:12.12 -> Cmd.stop @18:34:12.26 Carousel.seek 47 @18:34:12.43 Plunge.seek :locked @18:34:13.27 PinPort.seek :clear @18:34:13.40 Archive.seek :clear @18:34:13.53 Air.seek :clear @18:34:13.68 Lysis1.seek :clear @18:34:31.16 Archive.coast @18:34:31.21 Air.coast @18:34:31.27 Lysis1.coast @18:34:31.31 Lysis2.coast @18:34:31.36 Plunge.coast @18:34:31.42 SamplePump.coast @18:34:31.47 Toroid.coast @18:34:31.52 Carousel.coast @18:34:31.57 PinPort.coast @18:34:31.75 Gate.power :motor,:OFF @18:34:31.77 Gate.power :core,:OFF @18:34:31.79 Gate.power :analytic1,:OFF @18:34:31.81 Gate.power :analytic2,:OFF @18:34:31.83 Gate.power :analytic3,:OFF @18:34:31.85 Gate.power :main,:OFF @18:34:31.87 Safely stopped and ready to power off ##-- file closed 2019-08-19T22:50:22.238Z ##-- file opened 2019-08-19T22:50:22.259Z @18:50:23.02 -> showStatus @18:50:25.04 -> Cmd.loadCartridge -15 @18:50:25.19 Gate.power :main,:ON,limit to 5000mA @18:50:25.20 Gate.power :analytic1,:ON,limit to 5000mA @18:50:25.22 Gate.power :analytic2,:ON,limit to 5000mA @18:50:25.25 Gate.power :analytic3,:ON,limit to 5000mA @18:50:25.38 Powering core bus @18:50:27.66 SPR Module 1, FW Rev. B, FPGA Rev. C @18:50:27.80 Archive.configure :default @18:50:27.92 Air.configure :default @18:50:28.02 Lysis1.configure :default @18:50:28.12 Lysis2.configure :default @18:50:28.24 Plunge.configure :default @18:50:28.36 SamplePump.configure :default @18:50:28.48 ExtraPump.configure :default @18:50:28.60 Toroid.configure :default @18:50:28.72 Carousel.configure :default @18:50:28.84 Pressure.configure :default @18:50:28.94 PinPort.configure :default @18:50:29.00 All elf controllers running firmware version 4.58 @18:50:29.02 Gate.power :motor,:ON,limit to 5000mA @18:50:29.26 Archive.reconfigure :default @18:50:29.50 Archive.home.jog 0 @18:50:29.63 PinPort.reconfigure :default @18:50:29.71 Toroid.reconfigure :default @18:50:29.75 Lysis2.reconfigure :default @18:50:29.79 Air.reconfigure :default @18:50:29.83 Lysis1.reconfigure :default @18:50:29.87 Plunge.reconfigure :default @18:50:30.13 Lysis1.home.jog 0 @18:50:30.18 PinPort.home.jog 0 @18:50:30.24 Air.home.jog 0 @18:50:30.30 Lysis2.home.jog 0 @18:50:30.36 Plunge.home.jog 0 @18:50:30.41 Toroid.home.jog 0 @18:50:30.71 Archive.reconfigure :homing @18:50:30.94 Toroid.reconfigure :homing @18:50:30.96 Plunge.reconfigure :homing @18:50:30.99 Air.reconfigure :homing @18:50:31.03 PinPort.reconfigure :homing @18:50:31.06 Lysis2.reconfigure :homing @18:50:31.10 Lysis1.reconfigure :homing @18:50:31.33 Air.jog 1400 @18:50:31.38 Archive.jog 1381 @18:50:31.43 Toroid.jog 623 @18:50:31.49 PinPort.jog -106 @18:50:31.54 Plunge.jog 1546 @18:50:31.60 Lysis1.jog 1395 @18:50:31.65 Lysis2.jog 1426 @18:50:31.74 PinPort.setRawPosition! 0 @18:50:31.78 PinPort.seek :home @18:50:31.87 PinPort.reconfigure :default @18:50:32.48 Air.setRawPosition! 50 @18:50:32.53 Air.seek :home @18:50:32.61 Archive.setRawPosition! 50 @18:50:32.67 Archive.seek :home @18:50:32.77 Lysis1.setRawPosition! 50 @18:50:32.83 Lysis1.seek :home @18:50:32.92 Plunge.setRawPosition! 50 @18:50:32.95 Lysis2.setRawPosition! 50 @18:50:32.97 Air.reconfigure :default @18:50:33.07 Plunge.seek :home @18:50:33.12 Lysis2.seek :home @18:50:33.26 Lysis1.reconfigure :default @18:50:33.29 Archive.reconfigure :default @18:50:33.45 Lysis2.reconfigure :default @18:50:33.54 Plunge.reconfigure :default @18:50:33.60 Toroid.setRawPosition! 0 @18:50:33.65 Toroid.seek :home @18:50:33.74 Toroid.reconfigure :default @18:50:33.88 Toroid.seek :clear @18:50:35.50 Carousel.reconfigure :default @18:50:35.57 Carousel.home.jog 0 @18:50:36.07 Carousel.seek 47 @18:50:36.21 Selecting Cartridge 44 @18:50:36.45 Carousel.seek 44 @18:50:37.34 Lysis1.seek :locked @18:50:41.47 Plunge.seek :locked @18:50:41.59 Air.seek :locked @18:50:45.72 Powering cartridge bus @18:50:47.84 Gate.setIR 1750,5000,0,6 @18:50:47.97 Gate.setIR 0,5000,0,6 @18:50:48.02 Loaded Lyse-n-Go Cartridge @18:50:48.04 PuckHeater.configure HeatConfig @18:50:48.09 Gate.power :heat,:ON,limit to 5000mA @18:51:03.97 -> Cmd.startFiltering @18:51:04.57 Duration of filtering limited to 2:05:00 @18:51:04.83 Priming sample loop w/100ml, bypass w/10ml @18:51:04.84 SamplePump.setPosition! 0ml @18:51:04.89 Bypass.open @18:51:06.39 Intake.open @18:51:07.20 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:51:10.04 Vacuum tare is 52% of full scale @18:51:10.17 Pressure.reconfigure :default @18:51:10.23 Pressure.hold @18:51:10.40 SamplePump.reconfigure :prime @18:51:10.47 SamplePump.seek 10ml,1:21 @18:51:19.42 Pressure.coast @18:51:19.47 SamplePump.setPosition! 0ml @18:51:19.52 Exhaust.open @18:51:20.95 Bypass.close @18:51:22.48 Pressure.reconfigure :default @18:51:22.54 Pressure.hold @18:51:22.76 SamplePump.reconfigure :prime @18:51:22.83 SamplePump.seek 100ml,8:51 @18:53:16.62 Pressure.coast @18:53:16.68 Bypass.open @18:53:17.38 Intake.close @18:53:18.22 Toroid.reconfigure :hold @18:53:18.29 Toroid.seek :crack @18:53:19.08 Toroid.reconfigure :default @18:53:19.14 Toroid.seek :clear @18:53:20.70 Toroid.reconfigure :hold @18:53:20.77 Toroid.seek :cartridge @18:53:22.58 Toroid.reconfigure :default @18:53:22.64 Toroid.seek :clear @18:53:24.62 Pressurizing cartridge to 26psi above 15.9psia @18:53:24.63 SamplePump.setPosition! 0ml @18:53:24.67 Intake.open @18:53:25.40 Bypass.close @18:53:26.08 Exhaust.close @18:53:26.86 Pressure.reconfigure :default @18:53:26.91 Pressure.hold @18:53:27.08 SamplePump.reconfigure :charge @18:53:27.15 SamplePump.seek 2.3ml,42 seconds @18:53:38.00 Pressure.coast @18:53:38.05 Intake.close @18:53:38.86 Pressurized cartridge with 0.551ml to 26.0psi Isolated @38.7psia (22.8psi) @18:53:38.88 Checking for cartridge leaks from 38.7psia @18:53:38.91 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @18:53:44.03 Sampling 1000.0ml @18:53:44.04 SamplePump.setPosition! 0ml @18:53:44.09 Exhaust.open @18:53:44.77 Intake.open @18:53:45.67 Pressure.reconfigure :default @18:53:45.73 Pressure.hold @18:53:45.94 SamplePump.reconfigure :sample @18:53:46.02 SamplePump.seek 1000ml,1:23:53 @18:53:48.71 Cartridge 44 sampling 1/1000ml at 0.54ml/s with 23.5psi vacuum @18:54:48.71 Cartridge 44 sampling 34/1000ml at 0.56ml/s with 25.7psi vacuum @18:55:48.71 Cartridge 44 sampling 66/1000ml at 0.55ml/s with 24.1psi vacuum @18:56:48.71 Cartridge 44 sampling 98/1000ml at 0.52ml/s with 30.0psi vacuum @18:57:48.71 Cartridge 44 sampling 129/1000ml at 0.52ml/s with 23.5psi vacuum @18:58:48.71 Cartridge 44 sampling 158/1000ml at 0.48ml/s with 27.2psi vacuum @18:59:48.71 Cartridge 44 sampling 186/1000ml at 0.47ml/s with 26.0psi vacuum 1.7m deep (17.2psia) @19:00:48.71 Cartridge 44 sampling 212/1000ml at 0.41ml/s with 26.3psi vacuum @19:01:48.71 Cartridge 44 sampling 238/1000ml at 0.39ml/s with 23.4psi vacuum @19:02:48.71 Cartridge 44 sampling 262/1000ml at 0.37ml/s with 24.3psi vacuum @19:03:48.71 Cartridge 44 sampling 286/1000ml at 0.36ml/s with 24.2psi vacuum @19:04:48.71 Cartridge 44 sampling 309/1000ml at 0.37ml/s with 21.5psi vacuum @19:05:48.71 Cartridge 44 sampling 331/1000ml at 0.38ml/s with 25.4psi vacuum 1.9m deep (17.5psia) @19:06:48.71 Cartridge 44 sampling 352/1000ml at 0.34ml/s with 23.9psi vacuum @19:07:48.71 Cartridge 44 sampling 371/1000ml at 0.34ml/s with 26.1psi vacuum @19:08:48.71 Cartridge 44 sampling 391/1000ml at 0.34ml/s with 24.0psi vacuum @19:09:48.71 Cartridge 44 sampling 409/1000ml at 0.30ml/s with 23.9psi vacuum @19:10:48.70 Cartridge 44 sampling 426/1000ml at 0.25ml/s with 26.5psi vacuum @19:11:48.71 Cartridge 44 sampling 443/1000ml at 0.29ml/s with 23.9psi vacuum @19:12:48.71 Cartridge 44 sampling 458/1000ml at 0.23ml/s with 24.2psi vacuum @19:13:48.71 Cartridge 44 sampling 473/1000ml at 0.24ml/s with 21.3psi vacuum @19:14:48.71 Cartridge 44 sampling 488/1000ml at 0.25ml/s with 23.6psi vacuum @19:15:49.08 Cartridge 44 sampling 502/1000ml at 0.23ml/s with 23.7psi vacuum @19:16:48.71 Cartridge 44 sampling 515/1000ml at 0.20ml/s with 24.3psi vacuum @19:17:48.72 Cartridge 44 sampling 527/1000ml at 0.20ml/s with 27.0psi vacuum @19:18:48.71 Cartridge 44 sampling 540/1000ml at 0.20ml/s with 26.7psi vacuum @19:19:48.71 Cartridge 44 sampling 552/1000ml at 0.21ml/s with 22.6psi vacuum @19:20:48.70 Cartridge 44 sampling 564/1000ml at 0.20ml/s with 24.9psi vacuum @19:21:48.71 Cartridge 44 sampling 576/1000ml at 0.20ml/s with 26.5psi vacuum @19:22:48.71 Cartridge 44 sampling 589/1000ml at 0.20ml/s with 26.3psi vacuum @19:23:40.50 Flow averaged 0.33ml/s over 29:53 @19:23:40.53 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @19:23:41.23 Relieved excess vacuum in 2.3 seconds @19:23:41.25 SamplePump.seek 1000ml,33:57 @19:23:48.71 Cartridge 44 sampling 600/1000ml at 0.20ml/s with 30.0psi vacuum @19:24:21.21 Flow averaged 0.21ml/s over 38 seconds @19:24:21.24 Waiting up to 11 seconds for vacuum to reduce from 27.0 to 18.0psi @19:24:21.94 Relieved excess vacuum in 3.1 seconds @19:24:21.96 SamplePump.seek 1000ml,33:17 @19:24:41.24 Flow averaged 0.29ml/s over 14 seconds @19:24:41.27 Waiting up to 12 seconds for vacuum to reduce from 27.7 to 18.0psi @19:24:41.97 Relieved excess vacuum in 6.9 seconds @19:24:41.99 SamplePump.seek 1000ml,32:58 @19:24:48.71 Cartridge 44 sampling 612/1000ml at 0.20ml/s with 24.5psi vacuum @19:24:50.16 Flow estimate reduced to 0.23ml/s @19:24:50.18 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi @19:24:50.88 Relieved excess vacuum in 2.1 seconds @19:24:50.90 SamplePump.seek 1000ml,32:50 @19:25:03.64 Flow estimate reduced to 0.19ml/s @19:25:03.66 Intake.close @19:25:03.70 Exhaust.close @19:25:03.88 Toroid.reconfigure :hold @19:25:03.95 Toroid.seek :bypass @19:25:05.57 Toroid.reconfigure :default @19:25:05.64 Toroid.seek :clear @19:25:07.26 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @19:25:07.28 Pressure.coast @19:25:07.46 Sampled 614.8ml @19:25:09.38 -> Cmd.startProcessing @19:25:11.23 SPR.command \"read_sequences\" @19:25:11.26 SPR.command \"stats_on\" @19:25:11.30 SPR.command \"start 0 1\" @19:25:13.31 Begun log /var/log/esp/spr19aug1919h.spr @19:25:14.07 Handoff.select :waste @19:25:14.25 Archive.seek :empty @19:25:21.42 Syringe tare is 20% of full scale @19:25:31.97 PinPort.seek :cartridge @19:25:36.53 Lysis1.seek 1350ul @19:25:39.00 Lysis2.seek 1700ul @19:25:42.10 Plunge.seek :reagent @19:25:42.95 Evacuating puck with filter bubble point of 50psi @19:25:42.97 PinPort.seek :cartridge @19:25:43.05 Handoff.select :waste @19:25:50.38 Air pumping 1 strokes to reach 8psi @19:25:50.40 Air.reconfigure :gulp @19:25:50.47 Air.seek :tVent @19:25:52.29 0.2psi after gulp @19:25:52.30 Air stroke #1 of 1 @19:25:52.32 Air.reconfigure :pressurize @19:25:52.40 Air.seek :pressurized @19:26:05.24 0.1psi after pressurize @19:26:05.25 Air.reconfigure :compress @19:26:05.33 Air.seek :evac @19:26:08.00 0.5psi after compress @19:26:22.27 0.3psi after compress (stable) @19:26:22.40 at 0.4psi Opened to waste @19:26:22.41 Handoff.select :blocked @19:26:22.59 Air pumping 10 strokes to reach 50psi @19:26:22.60 Air.reconfigure :gulp @19:26:22.70 Air.seek :tVent @19:26:32.59 10.5psi after gulp @19:26:32.60 Air stroke #1 of 10 @19:26:32.62 Air.reconfigure :pressurize @19:26:32.70 Air.seek :pressurized @19:26:45.54 13.4psi after pressurize @19:26:45.55 Air.reconfigure :compress @19:26:45.64 Air.seek :evac @19:26:48.30 16.1psi after compress @19:27:16.63 35.6psi after compress (stable) @19:27:16.64 Air.reconfigure :gulp @19:27:16.73 Air.seek :tVent @19:27:26.63 36.8psi after gulp @19:27:26.64 Air stroke #2 of 10 @19:27:26.66 Air.reconfigure :pressurize @19:27:26.74 Air.seek :pressurized @19:27:39.57 34.3psi after pressurize @19:27:39.58 Air.reconfigure :compress @19:27:39.67 Air.seek :evac @19:27:42.33 34.6psi after compress @19:27:56.15 Reached 50.0 of 50.0psi after pumping 4672ul (3.00 strokes) @19:27:56.16 Handoff.select :waste @19:28:21.56 delay 30 seconds @19:28:51.59 Handoff.select :blocked @19:29:30.95 Handoff.select :waste @19:29:31.07 PSI at @4.45psi at evacEnd @19:29:31.08 Handoff.select :blocked @19:29:31.20 Air.reconfigure :gulp @19:29:31.31 Air.seek :tVent @19:29:48.38 Stable @3.8psi after Handoff blocked @19:29:48.47 Air pumping 10 strokes to reach 34psi @19:29:48.48 Air stroke #1 of 10 @19:29:48.49 Air.reconfigure :pressurize @19:29:48.58 Air.seek :pressurized @19:30:01.41 4.1psi after pressurize @19:30:01.42 Air.reconfigure :compress @19:30:01.50 Air.seek :evac @19:30:04.17 5.4psi after compress @19:30:26.75 16.1psi after compress (stable) @19:30:26.77 Air.reconfigure :gulp @19:30:26.85 Air.seek :tVent @19:30:36.74 16.8psi after gulp @19:30:36.75 Air stroke #2 of 10 @19:30:36.77 Air.reconfigure :pressurize @19:30:36.85 Air.seek :pressurized @19:30:49.69 16.8psi after pressurize @19:30:49.70 Air.reconfigure :compress @19:30:49.78 Air.seek :evac @19:30:52.44 18.7psi after compress @19:31:10.73 26.7psi after compress (stable) @19:31:10.75 Air.reconfigure :gulp @19:31:10.83 Air.seek :tVent @19:31:20.71 27.4psi after gulp @19:31:20.72 Air stroke #3 of 10 @19:31:20.74 Air.reconfigure :pressurize @19:31:20.82 Air.seek :pressurized @19:31:33.65 27.3psi after pressurize @19:31:33.66 Air.reconfigure :compress @19:31:33.74 Air.seek :evac @19:31:36.41 27.3psi after compress @19:31:39.76 Reached 33.9 of 33.8psi after pumping 6230ul (4.00 strokes) @19:31:39.77 Handoff.select :waste @19:31:56.35 delay 30 seconds @19:32:26.38 Handoff.select :blocked @19:32:51.36 Handoff.select :waste @19:32:51.51 PSI at @1.31psi at evacEnd @19:32:51.52 Air.reconfigure :default @19:32:51.64 Air.seek :fVent @19:32:53.07 delay 5 seconds @19:32:58.09 Seawater evacuated, Presure above/below filter released. Lysis begins @19:32:58.16 Lysis1.reconfigure :slow @19:32:58.22 Lysis1.seek 1050ul @19:33:28.24 delay 5 seconds @19:33:33.26 Handoff.select :blocked @19:33:33.42 Lysis1.seek 450ul @19:34:33.38 delay 3 seconds @19:34:36.45 Air.reconfigure to mix into 1.37806 parts Lysis1 @19:34:36.95 Air.seek :evac @19:34:36.99 Lysis1.seek 300ul @19:34:52.09 Air.reconfigure :default @19:34:52.15 Air.seek :full @19:35:06.36 Lysis1.seek :empty @19:35:36.46 Lysis Delivered. Syringe pressure @26.4psi @19:35:36.51 PuckHeater.seek 84C @19:36:26.05 PuckHeater.hold 15:00,84C @19:51:23.08 Lysis complete. Syringe pressure @33.8psi @19:51:23.09 Handoff.select :blocked @19:51:23.19 Lysis1.reconfigure :halfSpeed @19:51:23.26 Lysis1.seek 200ul @19:51:27.02 delay 1 second @19:51:28.04 Lysis1.seek :empty @19:51:31.87 Initial AirS pressure @34.0psi @19:51:31.93 Lysis1.reconfigure :extract @19:51:32.02 Lysis1.seek 900ul,10:00 @19:51:33.20 Air pumping 10 strokes to reach 21psi @19:51:33.21 Air stroke #1 of 10 @19:51:33.23 Air.reconfigure :pressurize @19:51:33.32 Air.seek :pressurized @19:51:46.15 16.2psi after pressurize @19:51:46.16 Air.reconfigure :compress @19:51:46.24 Air.seek :evac @19:51:48.90 16.6psi after compress @19:52:03.16 15.8psi after compress (stable) @19:52:03.18 Air.reconfigure :gulp @19:52:03.25 Air.seek :tVent @19:52:13.15 16.3psi after gulp @19:52:13.16 Air stroke #2 of 10 @19:52:13.18 Air.reconfigure :pressurize @19:52:13.25 Air.seek :pressurized @19:52:26.09 15.7psi after pressurize @19:52:26.13 Air.reconfigure :compress @19:52:26.21 Air.seek :evac @19:52:28.87 15.9psi after compress @19:52:34.46 Air.reconfigure :default @19:52:34.52 Air.hold @19:52:34.71 Lysis1 at 900ul @17.6psi @19:52:34.73 Lysis1.reconfigure :default @19:52:34.81 Lysis1.seek 925ul @19:52:35.52 Syringe::Overpressure in PROCESSING -- 20.1psi after recovery pull -- Skipping SPR @19:52:35.54 SPR.command \"start 2 14\" @19:52:36.97 Handoff.select :waste @19:52:37.47 Ended log /var/log/esp/spr19aug1919h.spr @19:52:37.48 SPR.command \"stats_off\" @19:52:39.11 -> Cmd.stop @19:52:39.21 Handoff.select :close @19:52:39.34 Gate.power :heat,:OFF @19:52:39.36 Gate.power :cartridge,:OFF @19:52:39.44 Carousel.seek 44 @19:52:39.62 Plunge.seek :locked @19:52:40.46 PinPort.seek :clear @19:52:45.07 Archive.seek :clear @19:52:45.19 Air.seek :clear @19:52:45.32 Lysis1.seek :clear @19:52:45.45 Lysis2.seek :clear @19:53:02.83 Archive.coast @19:53:02.88 Air.coast @19:53:02.93 Lysis1.coast @19:53:02.98 Lysis2.coast @19:53:03.03 Plunge.coast @19:53:03.08 SamplePump.coast @19:53:03.13 Toroid.coast @19:53:03.18 Carousel.coast @19:53:03.23 PinPort.coast @19:53:03.41 Gate.power :motor,:OFF @19:53:03.43 Gate.power :core,:OFF @19:53:03.45 Gate.power :analytic1,:OFF @19:53:03.47 Gate.power :analytic2,:OFF @19:53:03.49 Gate.power :analytic3,:OFF @19:53:03.51 Gate.power :main,:OFF @19:53:03.53 Safely stopped and ready to power off