##-- file opened 2019-07-27T02:28:10.493Z @19:28:11.43 -> showStatus @19:28:13.04 -> Cmd.loadCartridge -4 @19:28:13.17 Gate.power :main,:ON,limit to 5000mA @19:28:13.18 Gate.power :analytic1,:ON,limit to 5000mA @19:28:13.21 Gate.power :analytic2,:ON,limit to 5000mA @19:28:13.23 Gate.power :analytic3,:ON,limit to 5000mA @19:28:13.36 Powering core bus @19:28:15.65 SPR Module 1, FW Rev. B, FPGA Rev. C @19:28:15.77 Archive.configure :default @19:28:15.89 Air.configure :default @19:28:15.99 Lysis1.configure :default @19:28:16.09 Lysis2.configure :default @19:28:16.21 Plunge.configure :default @19:28:16.33 SamplePump.configure :default @19:28:16.46 ExtraPump.configure :default @19:28:16.58 Toroid.configure :default @19:28:16.70 Carousel.configure :default @19:28:16.81 Pressure.configure :default @19:28:16.92 PinPort.configure :default @19:28:16.97 All elf controllers running firmware version 4.58 @19:28:17.00 Gate.power :motor,:ON,limit to 5000mA @19:28:17.40 Toroid.reconfigure :default @19:28:17.48 Plunge.reconfigure :default @19:28:17.52 Lysis1.reconfigure :default @19:28:17.56 Archive.reconfigure :default @19:28:17.60 Air.reconfigure :default @19:28:17.64 Lysis2.reconfigure :default @19:28:17.70 PinPort.reconfigure :default @19:28:17.93 PinPort.home.jog 0 @19:28:17.97 Air.home.jog 0 @19:28:18.03 Archive.home.jog 0 @19:28:18.12 Lysis2.home.jog 0 @19:28:18.18 Lysis1.home.jog 0 @19:28:18.24 Plunge.home.jog 0 @19:28:18.30 Toroid.home.jog 0 @19:28:18.87 Toroid.reconfigure :homing @19:28:18.91 Plunge.reconfigure :homing @19:28:18.94 Archive.reconfigure :homing @19:28:18.98 PinPort.reconfigure :homing @19:28:19.01 Lysis1.reconfigure :homing @19:28:19.05 Air.reconfigure :homing @19:28:19.09 Lysis2.reconfigure :homing @19:28:19.31 Air.jog 1400 @19:28:19.37 Toroid.jog 629 @19:28:19.42 Plunge.jog 1532 @19:28:19.48 Lysis2.jog 1430 @19:28:19.54 PinPort.jog -106 @19:28:19.60 Archive.jog 1398 @19:28:19.66 Lysis1.jog 1398 @19:28:19.77 PinPort.setRawPosition! 0 @19:28:19.82 PinPort.seek :home @19:28:19.90 PinPort.reconfigure :default @19:28:20.47 Air.setRawPosition! 50 @19:28:20.51 Air.seek :home @19:28:20.64 Plunge.setRawPosition! 50 @19:28:20.68 Plunge.seek :home @19:28:20.80 Lysis2.setRawPosition! 50 @19:28:20.82 Air.reconfigure :default @19:28:20.85 Archive.setRawPosition! 50 @19:28:20.95 Lysis2.seek :home @19:28:21.01 Archive.seek :home @19:28:21.13 Plunge.reconfigure :default @19:28:21.16 Lysis1.setRawPosition! 50 @19:28:21.22 Lysis1.seek :home @19:28:21.34 Lysis2.reconfigure :default @19:28:21.37 Archive.reconfigure :default @19:28:21.51 Toroid.setRawPosition! 0 @19:28:21.56 Toroid.seek :home @19:28:21.66 Toroid.reconfigure :default @19:28:21.68 Lysis1.reconfigure :default @19:28:22.10 Toroid.seek :clear @19:28:23.72 Carousel.reconfigure :default @19:28:23.79 Carousel.home.jog 0 @19:28:24.28 Carousel.seek 22 @19:28:24.46 Selecting Cartridge 18 @19:28:24.70 Carousel.seek 18 @19:28:25.78 Lysis1.seek :locked @19:28:29.91 Plunge.seek :locked @19:28:30.03 Air.seek :locked @19:28:34.16 Powering cartridge bus @19:28:36.28 Gate.setIR 1750,5000,0,6 @19:28:36.41 Gate.setIR 0,5000,0,6 @19:28:36.45 Loaded Archive Cartridge Gate.power :cartridge,:OFF @19:28:51.46 -> Cmd.startFiltering @19:28:51.70 Duration of filtering limited to 2:05:00 @19:28:51.94 Priming sample loop w/100ml, bypass w/10ml @19:28:51.95 SamplePump.setPosition! 0ml @19:28:52.00 Bypass.open @19:28:53.47 Intake.open @19:28:54.27 Sea 5.3m deep (22.5psia) @19:28:54.29 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @19:28:57.13 Vacuum tare is 52% of full scale @19:28:57.26 Pressure.reconfigure :default @19:28:57.32 Pressure.hold @19:28:57.48 SamplePump.reconfigure :prime @19:28:57.56 SamplePump.seek 10ml,1:21 @19:29:06.52 Pressure.coast @19:29:06.57 SamplePump.setPosition! 0ml @19:29:06.98 Exhaust.open @19:29:08.41 Bypass.close @19:29:09.89 Pressure.reconfigure :default @19:29:09.95 Pressure.hold @19:29:10.06 Sea 5.1m deep (22.2psia) @19:29:10.19 SamplePump.reconfigure :prime @19:29:10.26 SamplePump.seek 100ml,8:51 @19:31:05.62 Pressure.coast @19:31:05.68 Bypass.open @19:31:06.37 Intake.close @19:31:07.18 Toroid.reconfigure :hold @19:31:07.24 Toroid.seek :crack @19:31:08.04 Toroid.reconfigure :default @19:31:08.10 Toroid.seek :clear @19:31:09.66 Toroid.reconfigure :hold @19:31:09.73 Toroid.seek :cartridge @19:31:11.54 Toroid.reconfigure :default @19:31:11.60 Toroid.seek :clear @19:31:13.56 Pressurizing cartridge to 26psi above 22.0psia @19:31:13.58 SamplePump.setPosition! 0ml @19:31:13.62 Intake.open @19:31:14.35 Bypass.close @19:31:15.06 Exhaust.close @19:31:15.83 Pressure.hold @19:31:16.00 SamplePump.reconfigure :charge @19:31:16.07 SamplePump.seek 2.3ml,42 seconds @19:31:24.04 Pressure.coast @19:31:24.09 Intake.close @19:31:24.91 Pressurized cartridge with 0.295ml to 26.1psi Isolated @44.1psia (22.1psi) @19:31:24.93 Checking for cartridge leaks from 44.1psia @19:31:24.96 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @19:31:30.05 Sampling 1000.0ml @19:31:30.07 SamplePump.setPosition! 0ml @19:31:30.12 Exhaust.open @19:31:30.79 Intake.open @19:31:31.64 Pressure.reconfigure :default @19:31:31.70 Pressure.hold @19:31:31.81 Sea 5.6m deep (23.0psia) @19:31:31.93 SamplePump.reconfigure :sample @19:31:32.01 SamplePump.seek 1000ml,1:23:53 @19:31:34.68 Cartridge 18 sampling 1/1000ml at 0.37ml/s with 23.8psi vacuum 2.7m deep (18.6psia) @19:32:34.68 Cartridge 18 sampling 23/1000ml at 0.38ml/s with 24.4psi vacuum 3.6m deep (20.0psia) @19:33:34.68 Cartridge 18 sampling 44/1000ml at 0.38ml/s with 23.5psi vacuum 4.1m deep (20.8psia) @19:34:34.68 Cartridge 18 sampling 65/1000ml at 0.36ml/s with 21.7psi vacuum 3.2m deep (19.5psia) @19:35:34.68 Cartridge 18 sampling 86/1000ml at 0.35ml/s with 21.4psi vacuum 3.6m deep (20.0psia) @19:36:34.68 Cartridge 18 sampling 105/1000ml at 0.36ml/s with 24.3psi vacuum 2.9m deep (18.9psia) @19:37:34.68 Cartridge 18 sampling 125/1000ml at 0.29ml/s with 27.6psi vacuum 2.6m deep (18.6psia) @19:38:34.68 Cartridge 18 sampling 143/1000ml at 0.30ml/s with 21.4psi vacuum 2.8m deep (18.8psia) @19:39:34.68 Cartridge 18 sampling 160/1000ml at 0.23ml/s with 18.6psi vacuum 4.1m deep (20.8psia) @19:40:34.68 Cartridge 18 sampling 173/1000ml at 0.22ml/s with 23.0psi vacuum 3.2m deep (19.4psia) @19:41:34.68 Cartridge 18 sampling 189/1000ml at 0.31ml/s with 25.7psi vacuum 3.4m deep (19.8psia) @19:42:34.68 Cartridge 18 sampling 206/1000ml at 0.28ml/s with 27.2psi vacuum 3.4m deep (19.8psia) @19:43:34.68 Cartridge 18 sampling 223/1000ml at 0.26ml/s with 28.0psi vacuum 2.1m deep (17.8psia) @19:44:34.68 Cartridge 18 sampling 239/1000ml at 0.30ml/s with 24.1psi vacuum 3.3m deep (19.6psia) @19:45:34.68 Cartridge 18 sampling 255/1000ml at 0.23ml/s with 20.9psi vacuum @19:46:34.68 Cartridge 18 sampling 271/1000ml at 0.27ml/s with 25.2psi vacuum @19:47:34.68 Cartridge 18 sampling 287/1000ml at 0.27ml/s with 22.6psi vacuum 3.3m deep (19.5psia) @19:48:34.68 Cartridge 18 sampling 301/1000ml at 0.20ml/s with 24.2psi vacuum 2.6m deep (18.5psia) @19:49:34.68 Cartridge 18 sampling 314/1000ml at 0.22ml/s with 26.5psi vacuum 3.4m deep (19.7psia) @19:50:34.68 Cartridge 18 sampling 327/1000ml at 0.20ml/s with 27.4psi vacuum 2.0m deep (17.6psia) @19:51:34.68 Cartridge 18 sampling 340/1000ml at 0.26ml/s with 25.6psi vacuum 3.0m deep (19.2psia) @19:52:34.68 Cartridge 18 sampling 354/1000ml at 0.26ml/s with 21.4psi vacuum 4.0m deep (20.6psia) @19:53:34.68 Cartridge 18 sampling 368/1000ml at 0.21ml/s with 24.3psi vacuum 4.2m deep (20.9psia) @19:54:34.68 Cartridge 18 sampling 381/1000ml at 0.24ml/s with 21.8psi vacuum 4.3m deep (21.0psia) @19:55:34.68 Cartridge 18 sampling 394/1000ml at 0.22ml/s with 21.1psi vacuum 4.5m deep (21.3psia) @19:56:34.68 Cartridge 18 sampling 406/1000ml at 0.21ml/s with 25.8psi vacuum 3.1m deep (19.3psia) @19:57:34.68 Cartridge 18 sampling 419/1000ml at 0.22ml/s with 20.2psi vacuum 4.5m deep (21.4psia) @19:58:34.68 Cartridge 18 sampling 432/1000ml at 0.23ml/s with 26.4psi vacuum 3.5m deep (19.8psia) @19:59:34.68 Cartridge 18 sampling 444/1000ml at 0.22ml/s with 26.3psi vacuum 2.4m deep (18.2psia) @20:00:34.68 Cartridge 18 sampling 457/1000ml at 0.20ml/s with 26.2psi vacuum 3.7m deep (20.1psia) @20:01:34.68 Cartridge 18 sampling 469/1000ml at 0.20ml/s with 30.6psi vacuum 2.4m deep (18.2psia) @20:02:34.68 Cartridge 18 sampling 481/1000ml at 0.20ml/s with 27.8psi vacuum 3.2m deep (19.4psia) @20:03:34.68 Cartridge 18 sampling 493/1000ml at 0.20ml/s with 27.2psi vacuum 3.1m deep (19.3psia) @20:04:34.68 Cartridge 18 sampling 506/1000ml at 0.20ml/s with 25.1psi vacuum 5.1m deep (22.3psia) @20:05:33.19 Flow averaged 0.25ml/s over 33:53 @20:05:33.22 Waiting up to 24 seconds for vacuum to reduce from 32.5 to 18.0psi @20:05:33.92 Relieved excess vacuum in 9.1 seconds @20:05:34.33 SamplePump.seek 1000ml,40:44 @20:05:34.67 Cartridge 18 sampling 517/1000ml at 0.10ml/s with 5.1psi vacuum 6.2m deep (23.9psia) @20:06:20.38 Flow averaged 0.21ml/s over 44 seconds @20:06:20.41 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @20:06:21.11 Relieved excess vacuum in 3.6 seconds @20:06:21.13 SamplePump.seek 1000ml,39:58 @20:06:32.41 Flow estimate reduced to 0.17ml/s @20:06:32.42 Intake.close @20:06:32.47 Exhaust.close @20:06:32.65 Toroid.reconfigure :hold @20:06:32.73 Toroid.seek :bypass @20:06:34.35 Toroid.reconfigure :default @20:06:34.42 Toroid.seek :clear @20:06:34.67 Cartridge 18 sampling 529/1000ml at 0.00ml/s with 0.3psi vacuum 7.1m deep (25.2psia) @20:06:36.04 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @20:06:36.06 Pressure.coast @20:06:36.24 Sampled 529.0ml @20:06:38.46 -> Cmd.startProcessing @20:06:38.70 Plunge.seek :reagent @20:06:39.56 Sample loop closed. Ready to Deliver Reagents @20:06:39.57 Archive.seek :sealed @20:06:43.84 Archive.seek :evac @20:06:46.01 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 1323ul (sensed 1353ul) @20:06:46.03 Retry #1 of 2 @20:06:46.04 Archive.hold @20:06:46.41 Archive.reconfigure :default @20:06:46.48 Archive.home.seek :evac @20:06:47.10 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 1311ul (sensed 1342ul) @20:06:47.12 Retry #2 of 2 @20:06:47.13 Archive.reconfigure :default @20:06:47.20 Archive.hold @20:06:47.54 Archive.reconfigure :default @20:06:47.61 Archive.home.seek :evac @20:06:48.23 Slide::Error in PROCESSING -- IGNORED Archive Syringe (ArS) positionErr at 1308ul (sensed 1338ul) @20:06:48.25 Archive.reconfigure :default @20:06:48.31 Archive.hold @20:06:48.67 Archive.reconfigure :default @20:06:48.74 Archive.home.jog 0 @20:06:48.86 ArchiveError in PROCESSING -- 1308ul RNAlater undelivered @20:06:48.89 Thread::ChildDied in LRAUV -- :PROCESSING ##-- file closed 2019-07-27T04:02:27.026Z ##-- file opened 2019-07-27T04:02:27.048Z @21:02:27.56 -> showStatus @21:02:29.57 -> Cmd.loadCartridge -4 @21:02:29.70 Gate.power :main,:ON,limit to 5000mA @21:02:29.71 Gate.power :analytic1,:ON,limit to 5000mA @21:02:29.74 Gate.power :analytic2,:ON,limit to 5000mA @21:02:29.76 Gate.power :analytic3,:ON,limit to 5000mA @21:02:29.89 Powering core bus @21:02:32.17 SPR Module 1, FW Rev. B, FPGA Rev. C @21:02:32.31 Archive.configure :default @21:02:32.43 Air.configure :default @21:02:32.53 Lysis1.configure :default @21:02:32.64 Lysis2.configure :default @21:02:32.75 Plunge.configure :default @21:02:32.87 SamplePump.configure :default @21:02:32.99 ExtraPump.configure :default @21:02:33.11 Toroid.configure :default @21:02:33.23 Carousel.configure :default @21:02:33.34 Pressure.configure :default @21:02:33.44 PinPort.configure :default @21:02:33.50 All elf controllers running firmware version 4.58 @21:02:33.52 Gate.power :motor,:ON,limit to 5000mA @21:02:33.67 Archive.reconfigure :default @21:02:33.98 Archive.home.jog 0 @21:02:34.12 Toroid.reconfigure :default @21:02:34.20 Plunge.reconfigure :default @21:02:34.24 Lysis2.reconfigure :default @21:02:34.28 Air.reconfigure :default @21:02:34.31 PinPort.reconfigure :default @21:02:34.38 Lysis1.reconfigure :default @21:02:34.60 Lysis1.home.jog 0 @21:02:34.65 Lysis2.home.jog 0 @21:02:34.70 Plunge.home.jog 0 @21:02:34.76 Toroid.home.jog 0 @21:02:34.82 Air.home.jog 0 @21:02:34.87 PinPort.home.jog 0 @21:02:35.11 Archive.reconfigure :homing @21:02:35.38 Archive.jog 1400 @21:02:35.54 Toroid.reconfigure :homing @21:02:35.58 Plunge.reconfigure :homing @21:02:35.61 Air.reconfigure :homing @21:02:35.65 PinPort.reconfigure :homing @21:02:35.68 Lysis2.reconfigure :homing @21:02:35.72 Lysis1.reconfigure :homing @21:02:35.95 Air.jog 1406 @21:02:36.00 Lysis2.jog 163 @21:02:36.06 PinPort.jog -105 @21:02:36.11 Toroid.jog 621 @21:02:36.17 Plunge.jog 1561 @21:02:36.23 Lysis1.jog 1406 @21:02:36.31 PinPort.setRawPosition! 0 @21:02:36.35 PinPort.seek :home @21:02:36.45 PinPort.reconfigure :default @21:02:36.48 Lysis2.setRawPosition! 50 @21:02:36.56 Lysis2.seek :home @21:02:36.64 Archive.setRawPosition! 50 @21:02:36.68 Archive.seek :home @21:02:36.84 Lysis2.reconfigure :default @21:02:36.96 Archive.reconfigure :default @21:02:37.10 Air.setRawPosition! 50 @21:02:37.14 Air.seek :home @21:02:37.40 Plunge.setRawPosition! 50 @21:02:37.46 Plunge.seek :home @21:02:37.54 Lysis1.setRawPosition! 50 @21:02:37.57 Air.reconfigure :default @21:02:37.64 Lysis1.seek :home @21:02:37.78 Plunge.reconfigure :default @21:02:37.96 Lysis1.reconfigure :default @21:02:38.22 Toroid.setRawPosition! 0 @21:02:38.26 Toroid.seek :home @21:02:38.35 Toroid.reconfigure :default @21:02:38.48 Toroid.seek :clear @21:02:40.10 Carousel.reconfigure :default @21:02:40.17 Carousel.home.jog 0 @21:02:40.66 Carousel.seek 18 @21:02:40.80 Selecting Cartridge 16 @21:02:41.04 Carousel.seek 16 @21:02:41.73 Lysis1.seek :locked @21:02:45.86 Plunge.seek :locked @21:02:45.98 Air.seek :locked @21:02:50.11 Powering cartridge bus @21:02:52.23 Gate.setIR 1750,5000,0,6 @21:02:52.36 Gate.setIR 0,5000,0,6 @21:02:52.40 Loaded Archive Cartridge @21:02:52.41 Gate.power :cartridge,:OFF @21:03:08.40 -> Cmd.startFiltering @21:03:08.63 Duration of filtering limited to 2:05:00 @21:03:08.88 Priming sample loop w/100ml, bypass w/10ml @21:03:08.89 SamplePump.setPosition! 0ml @21:03:08.93 Bypass.open @21:03:10.43 Intake.open @21:03:11.21 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:03:14.05 Vacuum tare is 51% of full scale @21:03:14.18 Pressure.reconfigure :default @21:03:14.24 Pressure.hold @21:03:14.40 SamplePump.reconfigure :prime @21:03:14.48 SamplePump.seek 10ml,1:21 @21:03:23.45 Pressure.coast @21:03:23.50 SamplePump.setPosition! 0ml @21:03:23.55 Exhaust.open @21:03:24.97 Bypass.close @21:03:26.52 Pressure.reconfigure :default @21:03:26.58 Pressure.hold @21:03:26.80 SamplePump.reconfigure :prime @21:03:26.87 SamplePump.seek 100ml,8:51 @21:05:23.59 Pressure.coast @21:05:23.64 Bypass.open @21:05:24.37 Intake.close @21:05:25.18 Toroid.reconfigure :hold @21:05:25.24 Toroid.seek :crack @21:05:26.04 Toroid.reconfigure :default @21:05:26.10 Toroid.seek :clear @21:05:27.66 Toroid.reconfigure :hold @21:05:27.73 Toroid.seek :cartridge @21:05:29.54 Toroid.reconfigure :default @21:05:29.60 Toroid.seek :clear @21:05:31.58 Pressurizing cartridge to 26psi above 21.4psia @21:05:31.59 SamplePump.setPosition! 0ml @21:05:31.63 Intake.open @21:05:32.36 Bypass.close @21:05:33.07 Exhaust.close @21:05:33.88 Pressure.reconfigure :default @21:05:33.94 Pressure.hold @21:05:34.10 SamplePump.reconfigure :charge @21:05:34.17 SamplePump.seek 2.3ml,42 seconds @21:05:41.01 Pressure.coast @21:05:41.06 Intake.close @21:05:41.85 Pressurized cartridge with 0.217ml to 26.1psi @21:05:41.86 Isolated @41.0psia (19.6psi) @21:05:41.87 Checking for cartridge leaks from 41.0psia @21:05:41.90 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @21:05:47.50 Cartridge pressure fell 8% to 37.9psia @21:05:47.58 Sampling 1000.0ml @21:05:47.59 SamplePump.setPosition! 0ml @21:05:47.65 Exhaust.open @21:05:48.33 Intake.open @21:05:49.17 Pressure.reconfigure :default @21:05:49.22 Pressure.hold @21:05:49.33 Sea 4.8m deep (21.8psia) @21:05:49.47 SamplePump.reconfigure :sample @21:05:49.54 SamplePump.seek 1000ml,1:23:53 @21:05:52.20 Cartridge 16 sampling 1/1000ml at 0.36ml/s with 25.3psi vacuum 1.9m deep (17.5psia) @21:06:52.21 Cartridge 16 sampling 20/1000ml at 0.33ml/s with 26.0psi vacuum @21:07:52.21 Cartridge 16 sampling 39/1000ml at 0.29ml/s with 23.1psi vacuum @21:08:52.21 Cartridge 16 sampling 56/1000ml at 0.28ml/s with 22.2psi vacuum @21:09:52.21 Cartridge 16 sampling 73/1000ml at 0.31ml/s with 23.5psi vacuum @21:10:52.21 Cartridge 16 sampling 89/1000ml at 0.30ml/s with 24.4psi vacuum @21:11:52.21 Cartridge 16 sampling 105/1000ml at 0.29ml/s with 24.0psi vacuum @21:12:52.21 Cartridge 16 sampling 119/1000ml at 0.27ml/s with 23.9psi vacuum @21:13:52.21 Cartridge 16 sampling 133/1000ml at 0.22ml/s with 23.6psi vacuum @21:14:52.21 Cartridge 16 sampling 146/1000ml at 0.20ml/s with 26.0psi vacuum @21:15:52.21 Cartridge 16 sampling 159/1000ml at 0.21ml/s with 24.0psi vacuum @21:16:07.81 Flow averaged 0.26ml/s over 10:13 @21:16:07.84 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @21:16:08.83 Relieved excess vacuum in 6.5 seconds @21:16:08.86 SamplePump.seek 1000ml,1:10:24 @21:16:10.78 Flow estimate reduced to 0.21ml/s @21:16:10.80 Waiting up to 20 seconds for vacuum to reduce from 30.9 to 18.0psi @21:16:11.80 Relieved excess vacuum in 2.8 seconds @21:16:11.83 SamplePump.seek 1000ml,1:10:22 @21:16:13.66 Flow estimate reduced to 0.17ml/s @21:16:13.68 Intake.close @21:16:13.74 Exhaust.close @21:16:13.90 Toroid.reconfigure :hold @21:16:13.97 Toroid.seek :bypass @21:16:15.59 Toroid.reconfigure :default @21:16:15.66 Toroid.seek :clear @21:16:17.28 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @21:16:17.30 Pressure.coast @21:16:17.48 Sampled 162.5ml @21:16:19.48 -> Cmd.startProcessing @21:16:19.74 Plunge.seek :reagent @21:16:20.59 Sample loop closed. Ready to Deliver Reagents @21:16:20.60 Archive.seek :sealed @21:16:24.88 Archive.seek :evac @21:16:38.83 delay 1:00 @21:17:38.85 Archive.seek :clear @21:17:56.60 RNAlater soak for 10:00 @21:27:56.62 Archive.seek :tVent @21:27:59.20 delay 1 second @21:28:00.22 Archive.seek :evac @21:28:15.85 delay 10 seconds @21:28:25.88 Archive.seek :tVent @21:28:41.50 delay 1 second @21:28:42.52 Archive.seek :evac @21:28:58.14 delay 10 seconds @21:29:08.17 Sample preserved and evacuated @21:29:09.13 -> Cmd.stop @21:29:09.27 Carousel.seek 16 @21:29:09.45 Plunge.seek :locked @21:29:10.28 PinPort.seek :clear @21:29:10.42 Archive.seek :clear @21:29:10.55 Air.seek :clear @21:29:10.68 Lysis1.seek :clear @21:29:28.17 Archive.coast @21:29:28.23 Air.coast @21:29:28.27 Lysis1.coast @21:29:28.32 Lysis2.coast @21:29:28.37 Plunge.coast @21:29:28.42 SamplePump.coast @21:29:28.47 Toroid.coast @21:29:28.52 Carousel.coast @21:29:28.58 PinPort.coast @21:29:28.76 Gate.power :motor,:OFF @21:29:28.78 Gate.power :core,:OFF @21:29:28.80 Gate.power :analytic1,:OFF @21:29:28.82 Gate.power :analytic2,:OFF @21:29:28.84 Gate.power :analytic3,:OFF @21:29:28.86 Gate.power :main,:OFF @21:29:28.88 Safely stopped and ready to power off ##-- file closed 2019-07-27T04:38:16.658Z ##-- file opened 2019-07-27T04:38:16.660Z @21:38:16.97 -> showStatus @21:38:18.99 -> Cmd.loadCartridge -9 @21:38:19.12 Gate.power :main,:ON,limit to 5000mA @21:38:19.14 Gate.power :analytic1,:ON,limit to 5000mA @21:38:19.16 Gate.power :analytic2,:ON,limit to 5000mA @21:38:19.18 Gate.power :analytic3,:ON,limit to 5000mA @21:38:19.31 Powering core bus @21:38:21.60 SPR Module 1, FW Rev. B, FPGA Rev. C @21:38:21.73 Archive.configure :default @21:38:21.86 Air.configure :default @21:38:21.97 Lysis1.configure :default @21:38:22.06 Lysis2.configure :default @21:38:22.18 Plunge.configure :default @21:38:22.30 SamplePump.configure :default @21:38:22.42 ExtraPump.configure :default @21:38:22.54 Toroid.configure :default @21:38:22.65 Carousel.configure :default @21:38:22.77 Pressure.configure :default @21:38:22.88 PinPort.configure :default @21:38:22.94 All elf controllers running firmware version 4.58 @21:38:22.96 Gate.power :motor,:ON,limit to 5000mA @21:38:23.12 Archive.reconfigure :default @21:38:23.42 Archive.home.jog 0 @21:38:23.55 Toroid.reconfigure :default @21:38:23.63 Plunge.reconfigure :default @21:38:23.66 Lysis1.reconfigure :default @21:38:23.70 Air.reconfigure :default @21:38:23.73 PinPort.reconfigure :default @21:38:23.80 Lysis2.reconfigure :default @21:38:24.06 Lysis2.home.jog 0 @21:38:24.11 PinPort.home.jog 0 @21:38:24.17 Plunge.home.jog 0 @21:38:24.23 Toroid.home.jog 0 @21:38:24.29 Air.home.jog 0 @21:38:24.34 Lysis1.home.jog 0 @21:38:24.65 Archive.reconfigure :homing @21:38:24.87 Plunge.reconfigure :homing @21:38:24.91 Lysis2.reconfigure :homing @21:38:24.94 Toroid.reconfigure :homing @21:38:24.98 Air.reconfigure :homing @21:38:25.01 PinPort.reconfigure :homing @21:38:25.05 Lysis1.reconfigure :homing @21:38:25.27 Lysis1.jog 1407 @21:38:25.32 Lysis2.jog 163 @21:38:25.38 PinPort.jog -106 @21:38:25.44 Toroid.jog 629 @21:38:25.49 Archive.jog 1412 @21:38:25.55 Plunge.jog 1561 @21:38:25.62 Air.jog 1412 @21:38:25.68 PinPort.setRawPosition! 0 @21:38:25.73 PinPort.seek :home @21:38:25.83 PinPort.reconfigure :default @21:38:25.85 Lysis2.setRawPosition! 50 @21:38:25.91 Lysis2.seek :home @21:38:26.21 Lysis2.reconfigure :default @21:38:26.42 Lysis1.setRawPosition! 50 @21:38:26.46 Lysis1.seek :home @21:38:26.65 Archive.setRawPosition! 50 @21:38:26.70 Archive.seek :home @21:38:26.82 Plunge.setRawPosition! 50 @21:38:26.85 Lysis1.reconfigure :default @21:38:26.87 Air.setRawPosition! 50 @21:38:26.97 Plunge.seek :home @21:38:27.00 Air.seek :home @21:38:27.18 Archive.reconfigure :default @21:38:27.36 Plunge.reconfigure :default @21:38:27.44 Air.reconfigure :default @21:38:27.57 Toroid.setRawPosition! 0 @21:38:27.61 Toroid.seek :home @21:38:27.70 Toroid.reconfigure :default @21:38:27.83 Toroid.seek :clear @21:38:29.45 Carousel.reconfigure :default @21:38:29.52 Carousel.home.jog 0 @21:38:30.02 Carousel.seek 16 @21:38:30.17 Selecting Cartridge 17 @21:38:30.41 Carousel.seek 17 @21:38:30.89 Lysis1.seek :locked @21:38:35.02 Plunge.seek :locked @21:38:35.14 Air.seek :locked @21:38:39.27 Powering cartridge bus @21:38:41.42 Gate.setIR 1750,5000,0,6 @21:38:41.55 Gate.setIR 0,5000,0,6 @21:38:41.60 Loaded Lyse-n-Go Cartridge @21:38:41.62 PuckHeater.configure HeatConfig @21:38:41.67 Gate.power :heat,:ON,limit to 5000mA @21:38:57.82 -> Cmd.startFiltering @21:38:58.05 Duration of filtering limited to 2:05:00 @21:38:58.31 Priming sample loop w/100ml, bypass w/10ml @21:38:58.32 SamplePump.setPosition! 0ml @21:38:58.36 Bypass.open @21:38:59.82 Intake.open @21:39:00.62 Sea 3.0m deep (19.2psia) @21:39:00.64 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:39:03.49 Vacuum tare is 52% of full scale @21:39:03.62 Pressure.reconfigure :default @21:39:03.68 Pressure.hold @21:39:04.20 SamplePump.reconfigure :prime @21:39:04.28 SamplePump.seek 10ml,1:10 @21:39:13.24 Pressure.coast @21:39:13.29 SamplePump.setPosition! 0ml @21:39:13.34 Exhaust.open @21:39:14.79 Bypass.close @21:39:16.29 Pressure.reconfigure :default @21:39:16.35 Pressure.hold @21:39:16.47 Sea 2.9m deep (19.0psia) @21:39:16.60 SamplePump.reconfigure :prime @21:39:16.67 SamplePump.seek 100ml,7:10 @21:41:12.97 Pressure.coast @21:41:13.03 Bypass.open @21:41:13.72 Intake.close @21:41:14.54 Toroid.reconfigure :hold @21:41:14.61 Toroid.seek :crack @21:41:15.40 Toroid.reconfigure :default @21:41:15.46 Toroid.seek :clear @21:41:17.03 Toroid.reconfigure :hold @21:41:17.09 Toroid.seek :cartridge @21:41:18.91 Toroid.reconfigure :default @21:41:18.97 Toroid.seek :clear @21:41:20.94 Pressurizing cartridge to 14psi above 19.0psia @21:41:20.96 SamplePump.setPosition! 0ml @21:41:21.00 Intake.open @21:41:21.74 Bypass.close @21:41:22.46 Exhaust.close @21:41:23.26 Pressure.reconfigure :default @21:41:23.31 Pressure.hold @21:41:23.48 SamplePump.reconfigure :charge @21:41:23.55 SamplePump.seek 2.3ml,41 seconds @21:41:32.21 Pressure.coast @21:41:32.26 Intake.close @21:41:33.09 Pressurized cartridge with 0.350ml to 14.1psi @21:41:33.10 Isolated @30.6psia (11.6psi) @21:41:33.11 Checking for cartridge leaks from 30.6psia @21:41:33.14 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @21:41:38.22 Sampling 1000.0ml @21:41:38.24 SamplePump.setPosition! 0ml @21:41:38.29 Exhaust.open @21:41:38.98 Intake.open @21:41:39.87 Pressure.reconfigure :default @21:41:39.93 Pressure.hold @21:41:40.04 Sea 3.0m deep (19.1psia) @21:41:40.16 SamplePump.reconfigure :sample @21:41:40.25 SamplePump.seek 1000ml,1:07:09 @21:41:42.91 Cartridge 17 sampling 1/1000ml at 0.38ml/s with 15.0psi vacuum @21:42:42.91 Cartridge 17 sampling 21/1000ml at 0.34ml/s with 14.8psi vacuum @21:43:42.91 Cartridge 17 sampling 42/1000ml at 0.35ml/s with 14.9psi vacuum 1.6m deep (17.1psia) @21:44:42.91 Cartridge 17 sampling 62/1000ml at 0.35ml/s with 13.3psi vacuum @21:45:42.91 Cartridge 17 sampling 82/1000ml at 0.35ml/s with 12.9psi vacuum @21:46:42.91 Cartridge 17 sampling 103/1000ml at 0.34ml/s with 13.1psi vacuum @21:47:42.91 Cartridge 17 sampling 123/1000ml at 0.32ml/s with 12.9psi vacuum 1.6m deep (17.1psia) @21:48:42.91 Cartridge 17 sampling 142/1000ml at 0.35ml/s with 13.6psi vacuum 2.2m deep (17.9psia) @21:49:42.91 Cartridge 17 sampling 162/1000ml at 0.32ml/s with 12.1psi vacuum @21:50:42.91 Cartridge 17 sampling 181/1000ml at 0.33ml/s with 15.2psi vacuum 1.8m deep (17.3psia) @21:51:42.91 Cartridge 17 sampling 200/1000ml at 0.30ml/s with 10.8psi vacuum 2.4m deep (18.2psia) @21:52:42.91 Cartridge 17 sampling 219/1000ml at 0.34ml/s with 13.0psi vacuum 2.1m deep (17.8psia) @21:53:42.91 Cartridge 17 sampling 238/1000ml at 0.32ml/s with 12.4psi vacuum 1.7m deep (17.2psia) @21:54:42.91 Cartridge 17 sampling 257/1000ml at 0.31ml/s with 12.3psi vacuum @21:55:42.91 Cartridge 17 sampling 276/1000ml at 0.32ml/s with 12.6psi vacuum 1.8m deep (17.4psia) @21:56:42.91 Cartridge 17 sampling 294/1000ml at 0.30ml/s with 12.0psi vacuum 1.7m deep (17.3psia) @21:57:42.91 Cartridge 17 sampling 312/1000ml at 0.29ml/s with 11.5psi vacuum @21:58:42.91 Cartridge 17 sampling 330/1000ml at 0.29ml/s with 15.1psi vacuum @21:59:42.91 Cartridge 17 sampling 348/1000ml at 0.29ml/s with 12.6psi vacuum @22:00:42.91 Cartridge 17 sampling 366/1000ml at 0.28ml/s with 12.6psi vacuum @22:01:42.91 Cartridge 17 sampling 384/1000ml at 0.31ml/s with 12.9psi vacuum 1.9m deep (17.6psia) @22:02:42.91 Cartridge 17 sampling 401/1000ml at 0.30ml/s with 11.6psi vacuum @22:03:42.91 Cartridge 17 sampling 419/1000ml at 0.28ml/s with 15.0psi vacuum @22:04:42.91 Cartridge 17 sampling 436/1000ml at 0.29ml/s with 15.3psi vacuum @22:05:42.91 Cartridge 17 sampling 453/1000ml at 0.28ml/s with 12.4psi vacuum @22:06:42.91 Cartridge 17 sampling 470/1000ml at 0.27ml/s with 11.8psi vacuum @22:07:42.91 Cartridge 17 sampling 487/1000ml at 0.29ml/s with 14.7psi vacuum 1.9m deep (17.5psia) @22:08:42.90 Cartridge 17 sampling 503/1000ml at 0.29ml/s with 14.9psi vacuum @22:09:42.91 Cartridge 17 sampling 520/1000ml at 0.27ml/s with 11.9psi vacuum @22:10:42.91 Cartridge 17 sampling 536/1000ml at 0.26ml/s with 11.9psi vacuum 1.6m deep (17.1psia) @22:11:42.91 Cartridge 17 sampling 552/1000ml at 0.25ml/s with 11.8psi vacuum @22:12:42.91 Cartridge 17 sampling 568/1000ml at 0.29ml/s with 13.0psi vacuum 2.3m deep (18.2psia) @22:13:42.90 Cartridge 17 sampling 584/1000ml at 0.26ml/s with 12.0psi vacuum 1.6m deep (17.1psia) @22:14:42.91 Cartridge 17 sampling 600/1000ml at 0.27ml/s with 13.4psi vacuum 2.2m deep (18.0psia) @22:15:42.93 Cartridge 17 sampling 617/1000ml at 0.25ml/s with 14.2psi vacuum @22:16:42.91 Cartridge 17 sampling 632/1000ml at 0.25ml/s with 15.0psi vacuum @22:17:42.91 Cartridge 17 sampling 648/1000ml at 0.28ml/s with 14.4psi vacuum @22:18:42.90 Cartridge 17 sampling 664/1000ml at 0.25ml/s with 15.4psi vacuum @22:19:42.91 Cartridge 17 sampling 680/1000ml at 0.26ml/s with 14.3psi vacuum @22:20:42.91 Cartridge 17 sampling 695/1000ml at 0.25ml/s with 14.3psi vacuum @22:21:42.91 Cartridge 17 sampling 710/1000ml at 0.25ml/s with 14.9psi vacuum @22:22:42.91 Cartridge 17 sampling 726/1000ml at 0.25ml/s with 15.3psi vacuum 1.7m deep (17.3psia) @22:23:42.90 Cartridge 17 sampling 741/1000ml at 0.25ml/s with 11.6psi vacuum 1.9m deep (17.5psia) @22:24:42.91 Cartridge 17 sampling 756/1000ml at 0.25ml/s with 15.6psi vacuum @22:25:42.91 Cartridge 17 sampling 771/1000ml at 0.26ml/s with 11.8psi vacuum 2.1m deep (17.9psia) @22:26:42.91 Cartridge 17 sampling 786/1000ml at 0.25ml/s with 16.6psi vacuum @22:26:56.21 Flow averaged 0.29ml/s over 45:13 @22:26:56.24 Waiting up to 7 seconds for vacuum to reduce from 14.7 to 10.0psi @22:26:56.94 Relieved excess vacuum in 3.9 seconds @22:26:56.96 SamplePump.seek 1000ml,14:32 @22:27:42.90 Cartridge 17 sampling 801/1000ml at 0.25ml/s with 14.4psi vacuum 1.7m deep (17.2psia) @22:27:53.09 Flow averaged 0.25ml/s over 56 seconds @22:27:53.12 Waiting up to 7 seconds for vacuum to reduce from 14.6 to 10.0psi @22:27:53.81 Relieved excess vacuum in .8 seconds @22:27:53.84 SamplePump.seek 1000ml,13:36 @22:27:59.19 Flow estimate reduced to 0.20ml/s @22:27:59.20 Intake.close @22:27:59.26 Exhaust.close @22:27:59.42 Toroid.reconfigure :hold @22:27:59.49 Toroid.seek :bypass @22:28:01.11 Toroid.reconfigure :default @22:28:01.18 Toroid.seek :clear @22:28:02.80 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @22:28:02.82 Pressure.coast @22:28:03.00 Sampled 804.9ml @22:28:05.05 -> Cmd.startProcessing @22:28:06.90 SPR.command \"read_sequences\" @22:28:07.48 SPR.command \"stats_on\" @22:28:07.52 SPR.command \"start 0 1\" @22:28:09.53 Begun log /var/log/esp/spr19jul2622h.spr @22:28:10.55 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @22:28:12.22 Handoff.select :waste @22:28:12.39 Archive.seek :empty @22:28:19.58 Syringe tare is 19% of full scale @22:28:30.11 PinPort.seek :cartridge @22:28:34.67 Lysis1.seek 1350ul @22:28:37.14 Lysis2.seek 1700ul @22:28:40.23 Plunge.seek :reagent @22:28:41.07 Evacuating puck with filter bubble point of 30psi @22:28:41.09 PinPort.seek :cartridge @22:28:41.18 Handoff.select :waste @22:28:48.50 Air pumping 1 strokes to reach 4psi @22:28:48.52 Air.reconfigure :gulp @22:28:48.61 Air.seek :tVent @22:28:50.40 0.4psi after gulp @22:28:50.41 Air stroke #1 of 1 @22:28:50.42 Air.reconfigure :pressurize @22:28:50.51 Air.seek :pressurized @22:29:03.35 0.3psi after pressurize @22:29:03.36 Air.reconfigure :compress @22:29:03.43 Air.seek :evac @22:29:06.10 0.8psi after compress @22:29:20.00 0.3psi after compress (stable) @22:29:20.12 at 0.3psi Opened to waste @22:29:20.13 Handoff.select :blocked @22:29:20.31 Air pumping 10 strokes to reach 30psi @22:29:20.32 Air.reconfigure :gulp @22:29:20.40 Air.seek :tVent @22:29:30.30 9.2psi after gulp @22:29:30.31 Air stroke #1 of 10 @22:29:30.33 Air.reconfigure :pressurize @22:29:30.41 Air.seek :pressurized @22:29:43.23 12.3psi after pressurize @22:29:43.24 Air.reconfigure :compress @22:29:43.32 Air.seek :evac @22:29:45.98 14.6psi after compress @22:29:56.08 Reached 30.0 of 30.0psi after pumping 3115ul (2.00 strokes) @22:29:56.10 Handoff.select :waste @22:30:07.86 delay 1:00 @22:31:07.88 Handoff.select :blocked @22:31:13.60 Pressure too High, open to waste again (4.1psi) @22:31:13.61 Handoff.select :waste @22:31:13.74 delay 1:00 @22:32:13.76 Handoff.select :blocked @22:32:39.15 Handoff.select :waste @22:32:39.27 PSI at @2.04psi at evacEnd @22:32:39.28 Handoff.select :blocked @22:32:39.41 Air.reconfigure :gulp @22:32:39.49 Air.seek :tVent @22:32:56.57 Stable @1.7psi after Handoff blocked @22:32:56.65 Air pumping 10 strokes to reach 27psi @22:32:56.67 Air stroke #1 of 10 @22:32:56.69 Air.reconfigure :pressurize @22:32:56.77 Air.seek :pressurized @22:33:09.60 2.0psi after pressurize @22:33:09.61 Air.reconfigure :compress @22:33:09.71 Air.seek :evac @22:33:12.37 3.1psi after compress @22:33:38.96 13.9psi after compress (stable) @22:33:38.99 Air.reconfigure :gulp @22:33:39.07 Air.seek :tVent @22:33:48.95 14.4psi after gulp @22:33:48.96 Air stroke #2 of 10 @22:33:48.98 Air.reconfigure :pressurize @22:33:49.05 Air.seek :pressurized @22:34:01.88 14.3psi after pressurize @22:34:01.89 Air.reconfigure :compress @22:34:01.97 Air.seek :evac @22:34:04.64 15.2psi after compress @22:34:29.94 24.8psi after compress (stable) @22:34:29.97 Air.reconfigure :gulp @22:34:30.06 Air.seek :tVent @22:34:39.94 25.2psi after gulp @22:34:39.95 Air stroke #3 of 10 @22:34:39.97 Air.reconfigure :pressurize @22:34:40.05 Air.seek :pressurized @22:34:52.89 25.0psi after pressurize @22:34:52.90 Air.reconfigure :compress @22:34:52.99 Air.seek :evac @22:34:55.66 25.5psi after compress @22:34:56.61 Reached 26.9 of 26.7psi after pumping 6230ul (4.00 strokes) @22:34:56.63 Handoff.select :waste @22:35:04.54 delay 1:00 @22:36:04.56 Handoff.select :blocked @22:36:29.60 Handoff.select :waste @22:36:29.72 PSI at @0.76psi at evacEnd @22:36:29.73 Air.reconfigure :default @22:36:29.82 Air.seek :fVent @22:36:31.25 delay 5 seconds @22:36:36.27 Seawater evacuated, Presure above/below filter released. Lysis begins @22:36:36.34 Lysis1.reconfigure :slow @22:36:36.40 Lysis1.seek 1050ul @22:37:06.43 delay 5 seconds @22:37:11.45 Handoff.select :blocked @22:37:11.61 Lysis1.seek 450ul @22:38:11.56 delay 3 seconds @22:38:15.00 Air.reconfigure to mix into 1.37806 parts Lysis1 @22:38:15.38 Air.seek :evac @22:38:15.42 Lysis1.seek 300ul @22:38:30.53 Air.reconfigure :default @22:38:30.59 Air.seek :full @22:38:44.81 Lysis1.seek :empty @22:39:14.89 Lysis Delivered. Syringe pressure @46.2psi @22:39:14.94 PuckHeater.seek 85.7C @22:40:23.37 PuckHeater.hold 15:00,85.7C @22:55:22.65 Lysis complete. Syringe pressure @57.1psi @22:55:22.66 Handoff.select :blocked @22:55:22.76 Lysis1.reconfigure :halfSpeed @22:55:22.82 Lysis1.seek 200ul @22:55:26.59 delay 1 second @22:55:27.61 Lysis1.seek :empty @22:55:31.42 Initial AirS pressure @57.3psi @22:55:31.49 Lysis1.reconfigure :extract @22:55:31.58 Lysis1.seek 875ul,10:00 @22:55:33.29 Air pumping 10 strokes to reach 21psi @22:55:33.30 Air stroke #1 of 10 @22:55:33.32 Air.reconfigure :pressurize @22:55:33.40 Air.seek :pressurized @22:55:46.59 16.1psi after pressurize @22:55:46.60 Air.reconfigure :compress @22:55:46.68 Air.seek :evac @22:55:49.36 17.9psi after compress @22:56:03.25 15.8psi after compress (stable) @22:56:03.27 Air.reconfigure :gulp @22:56:03.34 Air.seek :tVent @22:56:13.24 15.9psi after gulp @22:56:13.25 Air stroke #2 of 10 @22:56:13.26 Air.reconfigure :pressurize @22:56:13.35 Air.seek :pressurized @22:56:26.19 15.7psi after pressurize @22:56:26.20 Air.reconfigure :compress @22:56:26.28 Air.seek :evac @22:56:28.94 15.9psi after compress @22:56:42.84 15.9psi after compress (stable) @22:56:42.86 Air.reconfigure :gulp @22:56:42.93 Air.seek :tVent @22:56:43.10 Air.reconfigure :default @22:56:43.16 Air.hold @22:56:43.62 Lysis1 at 875ul pressure @16.7psi @22:56:43.64 Lysis1.reconfigure :default @22:56:43.73 Lysis1.seek 900ul @22:56:44.50 Lysis1 at 900ul pressure(finPSI) @11.2psi @22:56:44.51 delay 1 second @22:56:45.66 Lysis1 at 900ul pressure @12.9psi @22:56:45.68 Air.reconfigure :halfSpeed @22:56:45.76 Air.seek :fVent @22:56:48.25 Puck Top Vented. Syringe pressure @2.3psi @22:56:48.26 Handoff.select :waste @22:56:48.39 delay 5 seconds @22:56:53.41 Lysate Recovered in LyS1 @22:56:53.43 Lysis2.reconfigure :DAmix @22:56:53.54 Lysis1.reconfigure :DAmix @22:56:53.98 Lysis2.seek 1450ul @22:56:54.08 Lysis1.seek 650ul @22:57:29.09 delay 5 seconds @22:57:34.12 Handoff.select SPR @22:57:34.23 SPR.command \"start 2 14\" @22:57:35.35 delay 10 seconds @22:57:45.37 SPR.command \"start 1 0\" @22:57:49.08 Waiting up to 4:00 for SPR to enter state 1 @23:00:22.17 SPR.command \"hold_on\" @23:00:24.07 SPR Dilution 1 of 1 -- Running Experiment 1 @23:00:24.46 Lysis2.seek 1150ul @23:00:24.50 Lysis1.seek 350ul @23:01:06.51 volL1 = 300.0 and volL2 = 300.0 @23:01:06.53 delay 15 seconds @23:01:21.56 SPR.command \"hold_off\" @23:01:24.25 Waiting up to 25:00 for SPR to enter state 7 @23:21:37.15 SPR Analysis has Completed @23:21:37.16 Handoff.select :waste @23:21:38.09 Ended log /var/log/esp/spr19jul2622h.spr @23:21:38.10 SPR.command \"stats_off\" @23:21:39.60 -> Cmd.stop @23:21:39.69 Handoff.select :close @23:21:39.81 Gate.power :heat,:OFF @23:21:39.83 Gate.power :cartridge,:OFF @23:21:39.91 Carousel.seek 17 @23:21:40.09 Plunge.seek :locked @23:21:40.92 PinPort.seek :clear @23:21:45.54 Archive.seek :clear @23:21:45.66 Air.reconfigure :default @23:21:45.79 Lysis1.reconfigure :default @23:21:45.91 Air.seek :clear @23:21:45.97 Lysis1.seek :clear @23:21:46.05 Lysis2.reconfigure :default @23:21:46.12 Lysis2.seek :clear @23:22:03.29 Archive.coast @23:22:03.34 Air.coast @23:22:03.39 Lysis1.coast @23:22:03.44 Lysis2.coast @23:22:03.49 Plunge.coast @23:22:03.55 SamplePump.coast @23:22:03.94 Toroid.coast @23:22:03.99 Carousel.coast @23:22:04.04 PinPort.coast @23:22:04.23 Gate.power :motor,:OFF @23:22:04.24 Gate.power :core,:OFF @23:22:04.28 Gate.power :analytic1,:OFF @23:22:04.32 Gate.power :analytic2,:OFF @23:22:04.36 Gate.power :analytic3,:OFF @23:22:04.40 Gate.power :main,:OFF @23:22:04.41 Safely stopped and ready to power off ##-- file closed 2019-07-27T08:40:11.165Z ##-- file opened 2019-07-27T08:40:11.167Z @01:40:11.26 -> showStatus @01:40:13.28 -> Cmd.loadCartridge -4 @01:40:13.41 Gate.power :main,:ON,limit to 5000mA @01:40:13.42 Gate.power :analytic1,:ON,limit to 5000mA @01:40:13.45 Gate.power :analytic2,:ON,limit to 5000mA @01:40:13.47 Gate.power :analytic3,:ON,limit to 5000mA @01:40:13.60 Powering core bus @01:40:15.89 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:16.01 Archive.configure :default @01:40:16.13 Air.configure :default @01:40:16.23 Lysis1.configure :default @01:40:16.33 Lysis2.configure :default @01:40:16.44 Plunge.configure :default @01:40:16.56 SamplePump.configure :default @01:40:16.68 ExtraPump.configure :default @01:40:16.80 Toroid.configure :default @01:40:16.92 Carousel.configure :default @01:40:17.04 Pressure.configure :default @01:40:17.14 PinPort.configure :default @01:40:17.20 All elf controllers running firmware version 4.58 @01:40:17.22 Gate.power :motor,:ON,limit to 5000mA @01:40:17.44 Archive.reconfigure :default @01:40:17.66 Archive.home.jog 0 @01:40:17.79 PinPort.reconfigure :default @01:40:17.86 Plunge.reconfigure :default @01:40:17.89 Lysis1.reconfigure :default @01:40:17.93 Toroid.reconfigure :default @01:40:17.97 Air.reconfigure :default @01:40:18.01 Lysis2.reconfigure :default @01:40:18.24 Air.home.jog 0 @01:40:18.29 Lysis1.home.jog 0 @01:40:18.35 Toroid.home.jog 0 @01:40:18.41 PinPort.home.jog 0 @01:40:18.47 Lysis2.home.jog 0 @01:40:18.52 Plunge.home.jog 0 @01:40:19.13 Plunge.reconfigure :homing @01:40:19.15 Lysis2.reconfigure :homing @01:40:19.18 PinPort.reconfigure :homing @01:40:19.23 Lysis1.reconfigure :homing @01:40:19.26 Air.reconfigure :homing @01:40:19.30 Archive.reconfigure :homing @01:40:19.33 Toroid.reconfigure :homing @01:40:19.58 Lysis2.jog 165 @01:40:19.64 Archive.jog 1406 @01:40:19.70 PinPort.jog -105 @01:40:19.75 Air.jog 1413 @01:40:19.81 Plunge.jog 1558 @01:40:19.87 Lysis1.jog 1407 @01:40:19.94 Toroid.jog 630 @01:40:20.03 PinPort.setRawPosition! 0 @01:40:20.05 Lysis2.setRawPosition! 50 @01:40:20.11 PinPort.seek :home @01:40:20.15 Lysis2.seek :home @01:40:20.30 PinPort.reconfigure :default @01:40:20.47 Lysis2.reconfigure :default @01:40:20.80 Archive.setRawPosition! 50 @01:40:20.84 Archive.seek :home @01:40:20.93 Air.setRawPosition! 50 @01:40:20.97 Air.seek :home @01:40:21.07 Plunge.setRawPosition! 50 @01:40:21.08 Lysis1.setRawPosition! 50 @01:40:21.16 Plunge.seek :home @01:40:21.19 Lysis1.seek :home @01:40:21.33 Air.reconfigure :default @01:40:21.36 Archive.reconfigure :default @01:40:21.53 Plunge.reconfigure :default @01:40:21.60 Lysis1.reconfigure :default @01:40:22.09 Toroid.setRawPosition! 0 @01:40:22.13 Toroid.seek :home @01:40:22.22 Toroid.reconfigure :default @01:40:22.34 Toroid.seek :clear @01:40:23.96 Carousel.reconfigure :default @01:40:24.03 Carousel.home.jog 0 @01:40:24.52 Carousel.seek 17 @01:40:24.65 Selecting Cartridge 14 @01:40:24.88 Carousel.seek 14 @01:40:25.77 Lysis1.seek :locked @01:40:29.90 Plunge.seek :locked @01:40:30.02 Air.seek :locked @01:40:34.15 Powering cartridge bus @01:40:36.27 Gate.setIR 1750,5000,0,6 @01:40:36.40 Gate.setIR 0,5000,0,6 @01:40:36.44 Loaded Archive Cartridge Gate.power :cartridge,:OFF @01:40:51.67 -> Cmd.startFiltering @01:40:51.89 Duration of filtering limited to 2:05:00 @01:40:52.15 Priming sample loop w/100ml, bypass w/10ml @01:40:52.16 SamplePump.setPosition! 0ml @01:40:52.20 Bypass.open @01:40:53.67 Intake.open @01:40:54.48 Sea 5.3m deep (22.6psia) @01:40:54.50 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @01:40:57.34 Vacuum tare is 52% of full scale @01:40:57.47 Pressure.reconfigure :default @01:40:57.53 Pressure.hold @01:40:58.05 SamplePump.reconfigure :prime @01:40:58.13 SamplePump.seek 10ml,1:21 @01:41:07.11 Pressure.coast @01:41:07.16 SamplePump.setPosition! 0ml @01:41:07.20 Exhaust.open @01:41:08.59 Bypass.close @01:41:10.09 Pressure.reconfigure :default @01:41:10.15 Pressure.hold @01:41:10.27 Sea 5.5m deep (22.9psia) @01:41:10.40 SamplePump.reconfigure :prime @01:41:10.47 SamplePump.seek 100ml,8:51 @01:43:07.84 Pressure.coast @01:43:07.90 Bypass.open @01:43:08.57 Intake.close @01:43:09.39 Toroid.reconfigure :hold @01:43:09.45 Toroid.seek :crack @01:43:10.25 Toroid.reconfigure :default @01:43:10.31 Toroid.seek :clear @01:43:11.87 Toroid.reconfigure :hold @01:43:11.94 Toroid.seek :cartridge @01:43:13.75 Toroid.reconfigure :default @01:43:13.81 Toroid.seek :clear @01:43:15.78 Pressurizing cartridge to 26psi above 22.1psia @01:43:15.79 SamplePump.setPosition! 0ml @01:43:15.83 Intake.open @01:43:16.59 Bypass.close @01:43:17.27 Exhaust.close @01:43:18.03 Pressure.reconfigure :default @01:43:18.08 Pressure.hold @01:43:18.25 SamplePump.reconfigure :charge @01:43:18.32 SamplePump.seek 2.3ml,42 seconds @01:43:25.17 Pressure.coast @01:43:25.22 Intake.close @01:43:26.05 Pressurized cartridge with 0.217ml to 26.1psi @01:43:26.06 Isolated @44.5psia (22.4psi) @01:43:26.07 Checking for cartridge leaks from 44.5psia @01:43:26.10 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @01:43:31.18 Cartridge pressure fell 1% to 44.1psia @01:43:31.26 Sampling 1000.0ml @01:43:31.27 SamplePump.setPosition! 0ml @01:43:31.32 Exhaust.open @01:43:32.01 Intake.open @01:43:32.88 Pressure.reconfigure :default @01:43:32.94 Pressure.hold @01:43:33.05 Sea 5.5m deep (22.8psia) @01:43:33.19 SamplePump.reconfigure :sample @01:43:33.26 SamplePump.seek 1000ml,1:23:53 @01:43:35.91 Cartridge 14 sampling 1/1000ml at 0.36ml/s with 22.9psi vacuum 3.5m deep (19.9psia) @01:44:35.92 Cartridge 14 sampling 22/1000ml at 0.38ml/s with 26.9psi vacuum 3.2m deep (19.5psia) @01:45:35.92 Cartridge 14 sampling 43/1000ml at 0.38ml/s with 24.3psi vacuum 3.3m deep (19.5psia) @01:46:35.92 Cartridge 14 sampling 64/1000ml at 0.36ml/s with 27.5psi vacuum 3.4m deep (19.7psia) @01:47:35.92 Cartridge 14 sampling 84/1000ml at 0.37ml/s with 25.0psi vacuum 2.2m deep (18.0psia) @01:48:35.92 Cartridge 14 sampling 104/1000ml at 0.30ml/s with 22.9psi vacuum 2.9m deep (19.0psia) @01:49:35.92 Cartridge 14 sampling 123/1000ml at 0.29ml/s with 22.0psi vacuum 3.0m deep (19.1psia) @01:50:35.92 Cartridge 14 sampling 141/1000ml at 0.33ml/s with 26.0psi vacuum 3.3m deep (19.6psia) @01:51:35.92 Cartridge 14 sampling 160/1000ml at 0.27ml/s with 20.4psi vacuum 3.6m deep (20.0psia) @01:52:35.92 Cartridge 14 sampling 178/1000ml at 0.29ml/s with 22.4psi vacuum 3.0m deep (19.2psia) @01:53:35.92 Cartridge 14 sampling 195/1000ml at 0.31ml/s with 21.3psi vacuum 3.4m deep (19.7psia) @01:54:35.92 Cartridge 14 sampling 213/1000ml at 0.31ml/s with 23.5psi vacuum 2.8m deep (18.8psia) @01:55:35.92 Cartridge 14 sampling 229/1000ml at 0.31ml/s with 22.6psi vacuum 3.6m deep (20.1psia) @01:56:35.92 Cartridge 14 sampling 246/1000ml at 0.28ml/s with 27.4psi vacuum 2.6m deep (18.6psia) @01:57:35.92 Cartridge 14 sampling 262/1000ml at 0.23ml/s with 25.8psi vacuum 2.2m deep (18.0psia) @01:58:35.92 Cartridge 14 sampling 278/1000ml at 0.27ml/s with 22.7psi vacuum 2.5m deep (18.5psia) @01:59:35.92 Cartridge 14 sampling 294/1000ml at 0.29ml/s with 24.1psi vacuum 3.7m deep (20.2psia) @02:00:35.92 Cartridge 14 sampling 310/1000ml at 0.22ml/s with 21.0psi vacuum @02:01:35.92 Cartridge 14 sampling 325/1000ml at 0.27ml/s with 22.8psi vacuum 3.1m deep (19.2psia) @02:02:35.92 Cartridge 14 sampling 340/1000ml at 0.20ml/s with 23.1psi vacuum 2.1m deep (17.9psia) @02:03:35.92 Cartridge 14 sampling 354/1000ml at 0.27ml/s with 24.1psi vacuum 2.8m deep (18.8psia) @02:04:35.92 Cartridge 14 sampling 369/1000ml at 0.25ml/s with 25.4psi vacuum 2.6m deep (18.5psia) @02:05:35.92 Cartridge 14 sampling 383/1000ml at 0.25ml/s with 22.8psi vacuum 2.5m deep (18.3psia) @02:06:35.92 Cartridge 14 sampling 396/1000ml at 0.22ml/s with 25.4psi vacuum 3.5m deep (19.9psia) @02:07:35.92 Cartridge 14 sampling 410/1000ml at 0.24ml/s with 25.2psi vacuum 2.5m deep (18.3psia) @02:08:35.92 Cartridge 14 sampling 423/1000ml at 0.23ml/s with 23.9psi vacuum 3.4m deep (19.7psia) @02:09:35.92 Cartridge 14 sampling 437/1000ml at 0.23ml/s with 23.2psi vacuum 3.1m deep (19.2psia) @02:10:35.92 Cartridge 14 sampling 450/1000ml at 0.22ml/s with 23.5psi vacuum 2.8m deep (18.9psia) @02:11:35.92 Cartridge 14 sampling 462/1000ml at 0.22ml/s with 24.7psi vacuum 3.1m deep (19.3psia) @02:12:35.92 Cartridge 14 sampling 475/1000ml at 0.20ml/s with 26.0psi vacuum 2.5m deep (18.5psia) @02:13:35.92 Cartridge 14 sampling 487/1000ml at 0.20ml/s with 26.1psi vacuum 2.5m deep (18.3psia) @02:14:35.92 Cartridge 14 sampling 500/1000ml at 0.20ml/s with 27.4psi vacuum 2.7m deep (18.7psia) @02:15:35.92 Cartridge 14 sampling 512/1000ml at 0.20ml/s with 25.4psi vacuum 3.0m deep (19.2psia) @02:16:36.29 Cartridge 14 sampling 524/1000ml at 0.20ml/s with 27.0psi vacuum 2.1m deep (17.9psia) @02:17:35.92 Cartridge 14 sampling 537/1000ml at 0.20ml/s with 26.1psi vacuum 3.2m deep (19.5psia) @02:18:35.92 Cartridge 14 sampling 549/1000ml at 0.20ml/s with 25.3psi vacuum 3.3m deep (19.6psia) @02:19:35.92 Cartridge 14 sampling 561/1000ml at 0.20ml/s with 24.8psi vacuum 2.6m deep (18.6psia) @02:20:35.91 Cartridge 14 sampling 573/1000ml at 0.20ml/s with 27.4psi vacuum 2.1m deep (17.8psia) @02:21:27.15 Flow averaged 0.26ml/s over 37:53 @02:21:27.18 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @02:21:27.88 Relieved excess vacuum in 1.8 seconds @02:21:27.90 SamplePump.seek 1000ml,35:16 @02:21:32.78 Flow estimate reduced to 0.21ml/s @02:21:32.81 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi @02:21:33.51 Relieved excess vacuum in 2.5 seconds @02:21:33.53 SamplePump.seek 1000ml,35:12 @02:21:35.91 Cartridge 14 sampling 584/1000ml at 0.20ml/s with 28.0psi vacuum @02:22:08.69 Flow averaged 0.21ml/s over 34 seconds @02:22:08.72 Waiting up to 10 seconds for vacuum to reduce from 27.0 to 18.0psi @02:22:09.42 Relieved excess vacuum in 2.7 seconds @02:22:09.44 SamplePump.seek 1000ml,34:37 @02:22:35.91 Cartridge 14 sampling 596/1000ml at 0.20ml/s with 29.1psi vacuum 2.6m deep (18.5psia) @02:23:35.92 Cartridge 14 sampling 608/1000ml at 0.20ml/s with 27.7psi vacuum 2.5m deep (18.4psia) @02:24:10.24 Flow averaged 0.21ml/s over 1:57 @02:24:10.27 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi @02:24:10.97 Relieved excess vacuum in 4.9 seconds @02:24:10.99 SamplePump.seek 1000ml,32:35 @02:24:14.54 Flow estimate reduced to 0.17ml/s @02:24:14.57 Intake.close @02:24:14.62 Exhaust.close @02:24:14.79 Toroid.reconfigure :hold @02:24:14.86 Toroid.seek :bypass @02:24:16.47 Toroid.reconfigure :default @02:24:16.54 Toroid.seek :clear @02:24:18.16 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @02:24:18.18 Pressure.coast @02:24:18.36 Sampled 615.9ml @02:24:20.38 -> Cmd.startProcessing @02:24:20.63 Plunge.seek :reagent @02:24:21.49 Sample loop closed. Ready to Deliver Reagents @02:24:21.50 Archive.seek :sealed @02:24:25.77 Archive.seek :evac @02:24:39.72 delay 1:00 @02:25:39.74 Archive.seek :clear @02:25:57.47 RNAlater soak for 10:00 @02:35:57.49 Archive.seek :tVent @02:36:00.06 delay 1 second @02:36:01.08 Archive.seek :evac @02:36:16.72 delay 10 seconds @02:36:26.75 Archive.seek :tVent @02:36:42.38 delay 1 second @02:36:43.40 Archive.seek :evac @02:36:59.04 delay 10 seconds @02:37:09.07 Sample preserved and evacuated @02:37:09.93 -> Cmd.stop @02:37:10.07 Carousel.seek 14 @02:37:10.24 Plunge.seek :locked @02:37:11.07 PinPort.seek :clear @02:37:11.21 Archive.seek :clear @02:37:11.35 Air.seek :clear @02:37:11.48 Lysis1.seek :clear @02:37:28.96 Archive.coast @02:37:29.01 Air.coast @02:37:29.06 Lysis1.coast @02:37:29.11 Lysis2.coast @02:37:29.16 Plunge.coast @02:37:29.21 SamplePump.coast @02:37:29.26 Toroid.coast @02:37:29.31 Carousel.coast @02:37:29.36 PinPort.coast @02:37:29.54 Gate.power :motor,:OFF @02:37:29.55 Gate.power :core,:OFF @02:37:29.58 Gate.power :analytic1,:OFF @02:37:29.60 Gate.power :analytic2,:OFF @02:37:29.62 Gate.power :analytic3,:OFF @02:37:29.64 Gate.power :main,:OFF @02:37:29.66 Safely stopped and ready to power off ##-- file closed 2019-07-27T09:46:23.086Z ##-- file opened 2019-07-27T09:46:23.088Z @02:46:23.38 -> showStatus @02:46:25.40 -> Cmd.loadCartridge -9 @02:46:25.53 Gate.power :main,:ON,limit to 5000mA @02:46:25.54 Gate.power :analytic1,:ON,limit to 5000mA @02:46:25.56 Gate.power :analytic2,:ON,limit to 5000mA @02:46:25.59 Gate.power :analytic3,:ON,limit to 5000mA @02:46:25.72 Powering core bus @02:46:28.00 SPR Module 1, FW Rev. B, FPGA Rev. C @02:46:28.18 Archive.configure :default @02:46:28.30 Air.configure :default @02:46:28.41 Lysis1.configure :default @02:46:28.51 Lysis2.configure :default @02:46:28.63 Plunge.configure :default @02:46:28.75 SamplePump.configure :default @02:46:28.87 ExtraPump.configure :default @02:46:28.99 Toroid.configure :default @02:46:29.11 Carousel.configure :default @02:46:29.23 Pressure.configure :default @02:46:29.33 PinPort.configure :default @02:46:29.39 All elf controllers running firmware version 4.58 @02:46:29.41 Gate.power :motor,:ON,limit to 5000mA @02:46:29.64 Archive.reconfigure :default @02:46:29.87 Archive.home.jog 0 @02:46:30.01 Toroid.reconfigure :default @02:46:30.09 Plunge.reconfigure :default @02:46:30.12 Lysis1.reconfigure :default @02:46:30.21 Air.reconfigure :default @02:46:30.23 PinPort.reconfigure :default @02:46:30.26 Lysis2.reconfigure :default @02:46:30.50 Lysis2.home.jog 0 @02:46:30.55 Air.home.jog 0 @02:46:30.61 Toroid.home.jog 0 @02:46:30.67 PinPort.home.jog 0 @02:46:30.72 Plunge.home.jog 0 @02:46:30.78 Lysis1.home.jog 0 @02:46:31.09 Archive.reconfigure :homing @02:46:31.31 Toroid.reconfigure :homing @02:46:31.35 Plunge.reconfigure :homing @02:46:31.38 Lysis1.reconfigure :homing @02:46:31.42 Air.reconfigure :homing @02:46:31.46 PinPort.reconfigure :homing @02:46:31.49 Lysis2.reconfigure :homing @02:46:31.72 Air.jog 1409 @02:46:31.78 Toroid.jog 626 @02:46:31.83 Archive.jog 1408 @02:46:31.89 Lysis2.jog 165 @02:46:31.95 PinPort.jog -106 @02:46:32.01 Plunge.jog 1565 @02:46:32.07 Lysis1.jog 1410 @02:46:32.18 PinPort.setRawPosition! 0 @02:46:32.23 PinPort.seek :home @02:46:32.32 PinPort.reconfigure :default @02:46:32.40 Lysis2.setRawPosition! 50 @02:46:32.44 Lysis2.seek :home @02:46:32.73 Lysis2.reconfigure :default @02:46:32.88 Air.setRawPosition! 50 @02:46:32.93 Air.seek :home @02:46:33.03 Archive.setRawPosition! 50 @02:46:33.07 Archive.seek :home @02:46:33.25 Air.reconfigure :default @02:46:33.34 Plunge.setRawPosition! 50 @02:46:33.36 Lysis1.setRawPosition! 50 @02:46:33.42 Plunge.seek :home @02:46:33.51 Lysis1.seek :home @02:46:33.62 Archive.reconfigure :default @02:46:33.75 Plunge.reconfigure :default @02:46:33.83 Lysis1.reconfigure :default @02:46:33.92 Toroid.setRawPosition! 0 @02:46:33.97 Toroid.seek :home @02:46:34.05 Toroid.reconfigure :default @02:46:34.22 Toroid.seek :clear @02:46:35.84 Carousel.reconfigure :default @02:46:35.91 Carousel.home.jog 0 @02:46:36.41 Carousel.seek 14 @02:46:36.56 Selecting Cartridge 15 @02:46:36.81 Carousel.seek 15 @02:46:37.30 Lysis1.seek :locked @02:46:41.42 Plunge.seek :locked @02:46:41.54 Air.seek :locked @02:46:45.68 Powering cartridge bus @02:46:47.80 Gate.setIR 1750,5000,0,6 @02:46:47.93 Gate.setIR 0,5000,0,6 @02:46:47.98 Loaded Lyse-n-Go Cartridge @02:46:48.00 PuckHeater.configure HeatConfig @02:46:48.06 Gate.power :heat,:ON,limit to 5000mA @02:47:04.17 -> Cmd.startFiltering @02:47:04.40 Duration of filtering limited to 2:05:00 @02:47:04.65 Priming sample loop w/100ml, bypass w/10ml @02:47:04.67 SamplePump.setPosition! 0ml @02:47:04.72 Bypass.open @02:47:06.20 Intake.open @02:47:07.00 Sea 4.8m deep (21.8psia) @02:47:07.02 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @02:47:09.88 Vacuum tare is 51% of full scale @02:47:10.01 Pressure.reconfigure :default @02:47:10.07 Pressure.hold @02:47:10.60 SamplePump.reconfigure :prime @02:47:10.67 SamplePump.seek 10ml,1:10 @02:47:19.64 Pressure.coast @02:47:19.70 SamplePump.setPosition! 0ml @02:47:19.75 Exhaust.open @02:47:21.17 Bypass.close @02:47:22.73 Pressure.reconfigure :default @02:47:22.80 Pressure.hold @02:47:22.92 Sea 4.5m deep (21.4psia) @02:47:23.06 SamplePump.reconfigure :prime @02:47:23.14 SamplePump.seek 100ml,7:10 @02:49:19.87 Pressure.coast @02:49:19.93 Bypass.open @02:49:20.62 Intake.close @02:49:21.43 Toroid.reconfigure :hold @02:49:21.50 Toroid.seek :crack @02:49:22.30 Toroid.reconfigure :default @02:49:22.37 Toroid.seek :clear @02:49:23.92 Toroid.reconfigure :hold @02:49:23.99 Toroid.seek :cartridge @02:49:25.80 Toroid.reconfigure :default @02:49:25.86 Toroid.seek :clear @02:49:27.84 Pressurizing cartridge to 14psi above 21.6psia @02:49:27.85 SamplePump.setPosition! 0ml @02:49:27.90 Intake.open @02:49:28.62 Bypass.close @02:49:29.32 Exhaust.close @02:49:30.10 Pressure.reconfigure :default @02:49:30.16 Pressure.hold @02:49:30.33 SamplePump.reconfigure :charge @02:49:30.40 SamplePump.seek 2.3ml,41 seconds @02:49:39.08 Pressure.coast @02:49:39.13 Intake.close @02:49:39.94 Pressurized cartridge with 0.352ml to 14.3psi @02:49:39.95 Isolated @32.9psia (11.4psi) @02:49:39.96 Checking for cartridge leaks from 32.9psia @02:49:39.99 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @02:49:45.12 Sampling 1000.0ml @02:49:45.14 SamplePump.setPosition! 0ml @02:49:45.19 Exhaust.open @02:49:45.85 Intake.open @02:49:46.67 Pressure.reconfigure :default @02:49:46.73 Pressure.hold @02:49:46.84 Sea 4.8m deep (21.8psia) @02:49:46.98 SamplePump.reconfigure :sample @02:49:47.05 SamplePump.seek 1000ml,1:07:09 @02:49:49.71 Cartridge 15 sampling 1/1000ml at 0.40ml/s with 13.2psi vacuum 3.1m deep (19.2psia) @02:50:49.71 Cartridge 15 sampling 21/1000ml at 0.33ml/s with 12.6psi vacuum 3.2m deep (19.4psia) @02:51:49.71 Cartridge 15 sampling 41/1000ml at 0.33ml/s with 12.1psi vacuum 3.6m deep (20.0psia) @02:52:49.71 Cartridge 15 sampling 61/1000ml at 0.35ml/s with 14.8psi vacuum 3.3m deep (19.5psia) @02:53:49.71 Cartridge 15 sampling 81/1000ml at 0.34ml/s with 13.5psi vacuum 2.6m deep (18.6psia) @02:54:49.71 Cartridge 15 sampling 101/1000ml at 0.32ml/s with 12.1psi vacuum 3.3m deep (19.6psia) @02:55:49.71 Cartridge 15 sampling 120/1000ml at 0.31ml/s with 12.0psi vacuum 3.5m deep (19.9psia) @02:56:49.71 Cartridge 15 sampling 139/1000ml at 0.34ml/s with 14.2psi vacuum 3.7m deep (20.1psia) @02:57:49.71 Cartridge 15 sampling 159/1000ml at 0.31ml/s with 15.3psi vacuum 1.9m deep (17.5psia) @02:58:49.71 Cartridge 15 sampling 178/1000ml at 0.33ml/s with 12.7psi vacuum 3.8m deep (20.3psia) @02:59:49.71 Cartridge 15 sampling 196/1000ml at 0.33ml/s with 14.2psi vacuum 3.3m deep (19.5psia) @03:00:49.71 Cartridge 15 sampling 215/1000ml at 0.33ml/s with 13.1psi vacuum 3.4m deep (19.8psia) @03:01:49.71 Cartridge 15 sampling 234/1000ml at 0.30ml/s with 14.7psi vacuum 3.0m deep (19.1psia) @03:02:49.71 Cartridge 15 sampling 252/1000ml at 0.29ml/s with 11.9psi vacuum 3.4m deep (19.7psia) @03:03:49.71 Cartridge 15 sampling 270/1000ml at 0.30ml/s with 15.5psi vacuum 2.2m deep (18.0psia) @03:04:49.71 Cartridge 15 sampling 288/1000ml at 0.31ml/s with 11.5psi vacuum 3.8m deep (20.2psia) @03:05:49.71 Cartridge 15 sampling 305/1000ml at 0.28ml/s with 12.3psi vacuum 2.8m deep (18.9psia) @03:06:49.71 Cartridge 15 sampling 323/1000ml at 0.30ml/s with 11.9psi vacuum 4.3m deep (21.1psia) @03:07:49.71 Cartridge 15 sampling 340/1000ml at 0.28ml/s with 15.8psi vacuum 1.8m deep (17.3psia) @03:08:49.71 Cartridge 15 sampling 357/1000ml at 0.30ml/s with 13.6psi vacuum 3.0m deep (19.1psia) @03:09:49.71 Cartridge 15 sampling 373/1000ml at 0.27ml/s with 15.7psi vacuum 2.5m deep (18.5psia) @03:10:49.71 Cartridge 15 sampling 390/1000ml at 0.29ml/s with 11.4psi vacuum 3.8m deep (20.3psia) @03:11:49.70 Cartridge 15 sampling 406/1000ml at 0.29ml/s with 11.7psi vacuum 3.5m deep (19.9psia) @03:12:49.71 Cartridge 15 sampling 422/1000ml at 0.27ml/s with 12.5psi vacuum 2.9m deep (19.0psia) @03:13:49.71 Cartridge 15 sampling 438/1000ml at 0.25ml/s with 13.8psi vacuum 2.2m deep (17.9psia) @03:14:49.71 Cartridge 15 sampling 455/1000ml at 0.27ml/s with 12.9psi vacuum 2.9m deep (18.9psia) @03:15:49.71 Cartridge 15 sampling 470/1000ml at 0.27ml/s with 13.0psi vacuum 3.3m deep (19.6psia) @03:16:49.70 Cartridge 15 sampling 486/1000ml at 0.27ml/s with 12.8psi vacuum 3.2m deep (19.4psia) @03:17:49.71 Cartridge 15 sampling 502/1000ml at 0.25ml/s with 13.7psi vacuum 2.7m deep (18.7psia) @03:18:49.71 Cartridge 15 sampling 517/1000ml at 0.25ml/s with 14.3psi vacuum 3.1m deep (19.2psia) @03:19:49.71 Cartridge 15 sampling 533/1000ml at 0.25ml/s with 14.6psi vacuum 2.5m deep (18.4psia) @03:20:49.71 Cartridge 15 sampling 548/1000ml at 0.25ml/s with 12.0psi vacuum 3.0m deep (19.2psia) @03:21:49.70 Cartridge 15 sampling 563/1000ml at 0.25ml/s with 16.5psi vacuum 3.5m deep (19.8psia) @03:22:50.09 Cartridge 15 sampling 578/1000ml at 0.25ml/s with 13.9psi vacuum 3.7m deep (20.2psia) @03:23:07.59 Flow averaged 0.29ml/s over 33:14 @03:23:07.62 Waiting up to 9 seconds for vacuum to reduce from 15.5 to 10.0psi @03:23:08.33 Relieved excess vacuum in 8.1 seconds @03:23:08.35 SamplePump.seek 1000ml,28:20 @03:23:27.76 Flow averaged 0.37ml/s over 14 seconds @03:23:27.80 Waiting up to 8 seconds for vacuum to reduce from 14.8 to 10.0psi @03:23:28.50 Relieved excess vacuum in 7 seconds @03:23:28.52 SamplePump.seek 1000ml,28:01 @03:23:49.71 Cartridge 15 sampling 593/1000ml at 0.25ml/s with 15.4psi vacuum 3.7m deep (20.1psia) @03:23:51.57 Flow averaged 0.27ml/s over 22 seconds @03:23:51.60 Waiting up to 8 seconds for vacuum to reduce from 15.2 to 10.0psi @03:23:52.30 Relieved excess vacuum in 2.5 seconds @03:23:52.32 SamplePump.seek 1000ml,27:38 @03:24:04.19 Flow estimate reduced to 0.22ml/s @03:24:04.20 Intake.close @03:24:04.25 Exhaust.close @03:24:04.43 Toroid.reconfigure :hold @03:24:04.50 Toroid.seek :bypass @03:24:06.11 Toroid.reconfigure :default @03:24:06.18 Toroid.seek :clear @03:24:07.80 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @03:24:07.82 Pressure.coast @03:24:08.00 Sampled 595.9ml @03:24:09.92 -> Cmd.startProcessing @03:24:11.77 SPR.command \"read_sequences\" @03:24:12.22 SPR.command \"stats_on\" @03:24:12.26 SPR.command \"start 0 1\" @03:24:14.26 Begun log /var/log/esp/spr19jul2703h.spr @03:24:15.28 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @03:24:16.96 Handoff.select :waste @03:24:17.14 Archive.seek :empty @03:24:24.31 Syringe tare is 20% of full scale @03:24:34.86 PinPort.seek :cartridge @03:24:39.42 Lysis1.seek 1350ul @03:24:41.90 Lysis2.seek 1700ul @03:24:45.00 Plunge.seek :reagent @03:24:45.84 Evacuating puck with filter bubble point of 30psi @03:24:45.85 PinPort.seek :cartridge @03:24:45.94 Handoff.select :waste @03:24:53.28 Air pumping 1 strokes to reach 4psi @03:24:53.30 Air.reconfigure :gulp @03:24:53.39 Air.seek :tVent @03:24:55.17 -0.0psi after gulp @03:24:55.18 Air stroke #1 of 1 @03:24:55.20 Air.reconfigure :pressurize @03:24:55.30 Air.seek :pressurized @03:25:08.14 0.0psi after pressurize @03:25:08.15 Air.reconfigure :compress @03:25:08.23 Air.seek :evac @03:25:10.90 0.1psi after compress @03:25:24.79 0.0psi after compress (stable) @03:25:24.91 at 0.0psi Opened to waste @03:25:24.92 Handoff.select :blocked @03:25:25.10 Air pumping 10 strokes to reach 30psi @03:25:25.11 Air.reconfigure :gulp @03:25:25.19 Air.seek :tVent @03:25:35.08 1.0psi after gulp @03:25:35.09 Air stroke #1 of 10 @03:25:35.11 Air.reconfigure :pressurize @03:25:35.19 Air.seek :pressurized @03:25:48.03 1.4psi after pressurize @03:25:48.05 Air.reconfigure :compress @03:25:48.12 Air.seek :evac @03:25:50.79 1.5psi after compress @03:26:42.40 12.2psi after compress (stable) @03:26:42.42 Air.reconfigure :gulp @03:26:42.50 Air.seek :tVent @03:26:52.40 13.7psi after gulp @03:26:52.41 Air stroke #2 of 10 @03:26:52.43 Air.reconfigure :pressurize @03:26:52.51 Air.seek :pressurized @03:27:05.37 13.8psi after pressurize @03:27:05.38 Air.reconfigure :compress @03:27:05.46 Air.seek :evac @03:27:08.13 13.6psi after compress @03:27:47.86 23.4psi after compress (stable) @03:27:47.88 Air.reconfigure :gulp @03:27:47.96 Air.seek :tVent @03:27:57.86 25.3psi after gulp @03:27:57.88 Air stroke #3 of 10 @03:27:57.90 Air.reconfigure :pressurize @03:27:57.98 Air.seek :pressurized @03:28:10.83 24.7psi after pressurize @03:28:10.84 Air.reconfigure :compress @03:28:10.93 Air.seek :evac @03:28:13.60 23.8psi after compress @03:28:34.06 Reached 30.0 of 30.0psi after pumping 6230ul (4.00 strokes) @03:28:34.07 Handoff.select :waste @03:28:41.96 delay 1:00 @03:29:41.98 Handoff.select :blocked @03:30:01.86 Pressure too High, open to waste again (4.0psi) @03:30:01.88 Handoff.select :waste @03:30:02.00 delay 1:00 @03:31:02.02 Handoff.select :blocked @03:31:27.03 Handoff.select :waste @03:31:27.15 PSI at @1.54psi at evacEnd @03:31:27.16 Handoff.select :blocked @03:31:27.28 Air.reconfigure :gulp @03:31:27.36 Air.seek :tVent @03:31:44.46 Stable @1.4psi after Handoff blocked @03:31:44.54 Air pumping 10 strokes to reach 26psi @03:31:44.55 Air stroke #1 of 10 @03:31:44.58 Air.reconfigure :pressurize @03:31:44.66 Air.seek :pressurized @03:31:57.50 1.8psi after pressurize @03:31:57.51 Air.reconfigure :compress @03:31:57.59 Air.seek :evac @03:32:00.26 2.0psi after compress @03:32:30.03 12.4psi after compress (stable) @03:32:30.06 Air.reconfigure :gulp @03:32:30.14 Air.seek :tVent @03:32:40.05 13.2psi after gulp @03:32:40.06 Air stroke #2 of 10 @03:32:40.08 Air.reconfigure :pressurize @03:32:40.16 Air.seek :pressurized @03:32:53.00 13.3psi after pressurize @03:32:53.02 Air.reconfigure :compress @03:32:53.10 Air.seek :evac @03:32:55.79 13.6psi after compress @03:33:23.61 23.0psi after compress (stable) @03:33:23.63 Air.reconfigure :gulp @03:33:23.70 Air.seek :tVent @03:33:33.61 23.9psi after gulp @03:33:33.62 Air stroke #3 of 10 @03:33:33.64 Air.reconfigure :pressurize @03:33:33.73 Air.seek :pressurized @03:33:46.56 23.7psi after pressurize @03:33:46.58 Air.reconfigure :compress @03:33:46.65 Air.seek :evac @03:33:49.32 23.9psi after compress @03:33:53.07 Reached 26.5 of 26.4psi after pumping 6230ul (4.00 strokes) @03:33:53.08 Handoff.select :waste @03:34:00.81 delay 1:00 @03:35:00.83 Handoff.select :blocked @03:35:27.25 Handoff.select :waste @03:35:27.37 PSI at @3.62psi at evacEnd @03:35:27.38 Air.reconfigure :default @03:35:27.47 Air.seek :fVent @03:35:28.90 delay 5 seconds @03:35:33.92 Seawater evacuated, Presure above/below filter released. Lysis begins @03:35:33.99 Lysis1.reconfigure :slow @03:35:34.05 Lysis1.seek 1050ul @03:36:04.08 delay 5 seconds @03:36:09.10 Handoff.select :blocked @03:36:09.28 Lysis1.seek 450ul @03:37:09.26 delay 3 seconds @03:37:12.33 Air.reconfigure to mix into 1.37806 parts Lysis1 @03:37:12.82 Air.seek :evac @03:37:12.85 Lysis1.seek 300ul @03:37:27.96 Air.reconfigure :default @03:37:28.02 Air.seek :full @03:37:42.25 Lysis1.seek :empty @03:38:12.38 Lysis Delivered. Syringe pressure @34.1psi @03:38:12.42 PuckHeater.seek 85.8C @03:39:25.03 PuckHeater.hold 15:00,85.8C @03:54:22.93 Lysis complete. Syringe pressure @31.7psi @03:54:22.94 Handoff.select :blocked @03:54:23.06 Lysis1.reconfigure :halfSpeed @03:54:23.13 Lysis1.seek 200ul @03:54:26.90 delay 1 second @03:54:27.92 Lysis1.seek :empty @03:54:31.74 Initial AirS pressure @31.5psi @03:54:31.80 Lysis1.reconfigure :extract @03:54:31.89 Lysis1.seek 875ul,10:00 @03:54:33.05 Air pumping 10 strokes to reach 21psi @03:54:33.06 Air stroke #1 of 10 @03:54:33.44 Air.reconfigure :pressurize @03:54:33.52 Air.seek :pressurized @03:54:46.36 12.8psi after pressurize @03:54:46.37 Air.reconfigure :compress @03:54:46.45 Air.seek :evac @03:54:49.13 18.3psi after compress @03:55:03.05 15.7psi after compress (stable) @03:55:03.07 Air.reconfigure :gulp @03:55:03.14 Air.seek :tVent @03:55:13.04 16.0psi after gulp @03:55:13.05 Air stroke #2 of 10 @03:55:13.07 Air.reconfigure :pressurize @03:55:13.16 Air.seek :pressurized @03:55:26.00 15.7psi after pressurize @03:55:26.01 Air.reconfigure :compress @03:55:26.09 Air.seek :evac @03:55:28.76 15.8psi after compress @03:55:42.64 15.9psi after compress (stable) @03:55:42.66 Air.reconfigure :gulp @03:55:42.73 Air.seek :tVent @03:55:52.64 15.3psi after gulp @03:55:52.65 Air stroke #3 of 10 @03:55:52.67 Air.reconfigure :pressurize @03:55:52.75 Air.seek :pressurized @03:56:05.58 16.1psi after pressurize @03:56:05.59 Air.reconfigure :compress @03:56:05.66 Air.seek :evac @03:56:08.33 16.6psi after compress @03:56:22.42 15.7psi after compress (stable) @03:56:22.44 Air.reconfigure :gulp @03:56:22.51 Air.seek :tVent @03:56:32.41 16.4psi after gulp @03:56:32.42 Air stroke #4 of 10 @03:56:32.44 Air.reconfigure :pressurize @03:56:32.52 Air.seek :pressurized @03:56:45.36 15.7psi after pressurize @03:56:45.37 Air.reconfigure :compress @03:56:45.45 Air.seek :evac @03:56:48.13 16.1psi after compress @03:56:59.87 Air.reconfigure :default @03:56:59.92 Air.hold @03:57:00.12 Lysis1 at 875ul pressure @15.0psi @03:57:00.14 Lysis1.reconfigure :default @03:57:00.22 Lysis1.seek 900ul @03:57:01.01 Lysis1 at 900ul pressure(finPSI) @9.9psi @03:57:01.02 delay 1 second @03:57:02.13 Lysis1 at 900ul pressure @11.7psi @03:57:02.15 Air.reconfigure :halfSpeed @03:57:02.22 Air.seek :fVent @03:57:04.47 Puck Top Vented. Syringe pressure @0.8psi @03:57:04.48 Handoff.select :waste @03:57:04.60 delay 5 seconds @03:57:09.62 Lysate Recovered in LyS1 @03:57:09.63 Lysis2.reconfigure :DAmix @03:57:09.75 Lysis1.reconfigure :DAmix @03:57:10.12 Lysis2.seek 1450ul @03:57:10.20 Lysis1.seek 650ul @03:57:45.24 delay 5 seconds @03:57:50.26 Handoff.select SPR @03:57:50.37 SPR.command \"start 2 14\" @03:57:51.68 delay 10 seconds @03:58:01.70 SPR.command \"start 1 0\" @03:58:04.93 Waiting up to 4:00 for SPR to enter state 1 @04:00:37.56 SPR.command \"hold_on\" @04:00:38.99 SPR Dilution 1 of 1 -- Running Experiment 1 @04:00:39.40 Lysis2.seek 1150ul @04:00:39.46 Lysis1.seek 350ul @04:01:21.50 volL1 = 300.0 and volL2 = 300.0 @04:01:21.51 delay 15 seconds @04:01:36.53 SPR.command \"hold_off\" @04:01:39.64 Waiting up to 25:00 for SPR to enter state 7 @04:21:53.48 SPR Analysis has Completed @04:21:53.49 Handoff.select :waste @04:21:54.42 Ended log /var/log/esp/spr19jul2703h.spr @04:21:54.43 SPR.command \"stats_off\" @04:21:56.14 -> Cmd.stop @04:21:56.23 Handoff.select :close @04:21:56.35 Gate.power :heat,:OFF @04:21:56.37 Gate.power :cartridge,:OFF @04:21:56.45 Carousel.seek 15 @04:21:56.63 Plunge.seek :locked @04:21:57.46 PinPort.seek :clear @04:22:02.08 Archive.seek :clear @04:22:02.21 Air.reconfigure :default @04:22:02.34 Lysis1.reconfigure :default @04:22:02.44 Air.seek :clear @04:22:02.50 Lysis1.seek :clear @04:22:02.59 Lysis2.reconfigure :default @04:22:02.67 Lysis2.seek :clear @04:22:19.84 Archive.coast @04:22:19.89 Air.coast @04:22:19.94 Lysis1.coast @04:22:19.99 Lysis2.coast @04:22:20.04 Plunge.coast @04:22:20.09 SamplePump.coast @04:22:20.49 Toroid.coast @04:22:20.54 Carousel.coast @04:22:20.59 PinPort.coast @04:22:20.77 Gate.power :motor,:OFF @04:22:20.79 Gate.power :core,:OFF @04:22:20.81 Gate.power :analytic1,:OFF @04:22:20.83 Gate.power :analytic2,:OFF @04:22:20.85 Gate.power :analytic3,:OFF @04:22:20.87 Gate.power :main,:OFF @04:22:20.89 Safely stopped and ready to power off ##-- file closed 2019-07-28T04:09:50.814Z