##-- file opened 2018-08-29T14:58:11.206Z @10:58:06.14 -> showStatus @10:58:07.79 -> Cmd.loadCartridge @10:58:07.91 Gate.power :main,:ON,limit to 5000mA @10:58:07.93 Gate.power :analytic1,:ON,limit to 5000mA @10:58:07.95 Gate.power :analytic2,:ON,limit to 5000mA @10:58:07.97 Gate.power :analytic3,:ON,limit to 5000mA @10:58:08.11 Powering core bus @10:58:10.41 SPR Module 1, FW Rev. B, FPGA Rev. C @10:58:10.51 ArS.configure ArSconfig @10:58:10.61 LyS1.configure LyS1config @10:58:10.72 LyS2.configure LyS2config @10:58:10.83 AirS.configure AirSconfig @10:58:10.95 TV.configure TVconfig @10:58:11.06 SP.configure SPconfig @10:58:11.19 SC.configure SCconfig @10:58:11.31 PV.configure PVconfig @10:58:11.43 PinPort.configure PPconfig @10:58:11.55 Sea.configure SPconfig @10:58:11.61 All elf controllers running firmware version 4.5 @10:58:11.62 Gate.power :motor,:ON,limit to 5000mA @10:58:11.93 ArS.reconfigure ArSconfig @10:58:12.15 PV.reconfigure PVconfig @10:58:12.21 TV.reconfigure TVconfig @10:58:12.25 ArS.home.jog 0 @10:58:12.29 LyS2.reconfigure LyS2config @10:58:12.32 PinPort.reconfigure PPconfig @10:58:12.34 AirS.reconfigure AirSconfig @10:58:12.42 LyS1.reconfigure LyS1config @10:58:12.56 PV.home.jog 0 @10:58:12.61 PinPort.home.jog 0 @10:58:12.66 TV.home.jog 0 @10:58:12.67 AirS.home.jog 0 @10:58:12.71 LyS2.home.jog 0 @10:58:12.75 LyS1.home.jog 0 @10:58:13.39 PV.reconfigure HomePV @10:58:13.42 TV.reconfigure HomeTV @10:58:13.51 ArS.reconfigure HomeMagSyringe @10:58:13.53 AirS.reconfigure HomeMagSyringe @10:58:13.57 PinPort.reconfigure HomePP @10:58:13.74 PV.jog -1474 @10:58:13.81 TV.jog -535 @10:58:13.83 PinPort.jog -111 @10:58:13.88 AirS.jog -314 @10:58:13.93 ArS.jog -226 @10:58:14.14 LyS1.reconfigure HomeSyringe @10:58:14.16 LyS2.reconfigure HomeSyringe @10:58:14.25 LyS1.jog -263 @10:58:14.29 LyS2.jog -259 @10:58:14.40 PinPort.setRawPosition! 0 @10:58:14.45 PinPort.seek :home @10:58:14.80 AirS.setRawPosition! -50 @10:58:14.85 ArS.setRawPosition! -50 @10:58:14.88 PinPort.reconfigure PPconfig @10:58:14.96 AirS.seek :home @10:58:15.02 ArS.seek :home @10:58:15.16 PV.setRawPosition! -50 @10:58:15.18 LyS2.setRawPosition! -50 @10:58:15.20 LyS1.setRawPosition! -50 @10:58:15.33 PV.seek :home @10:58:15.36 LyS2.seek :home @10:58:15.38 LyS1.seek :home @10:58:15.57 AirS.reconfigure AirSconfig @10:58:15.59 ArS.reconfigure ArSconfig @10:58:15.77 PV.reconfigure PVconfig @10:58:15.81 LyS2.reconfigure LyS2config @10:58:15.92 TV.setRawPosition! 0 @10:58:15.94 LyS1.reconfigure LyS1config @10:58:16.02 TV.seek :home @10:58:16.14 TV.reconfigure TVconfig @10:58:16.51 TV.seek :clear @10:58:18.13 TV.coast @10:58:18.19 SC.reconfigure HomeSC @10:58:18.25 SC.home.jog 0 @10:58:19.00 SC.reconfigure SCconfig @10:58:19.07 SC.seek 31 @10:58:19.16 Selecting Cartridge 30 @10:58:19.46 SC.seek 30 @10:58:19.96 LyS2.seek :locked @10:58:20.09 PV.seek :locked @10:58:20.23 AirS.seek :locked @10:58:24.30 Powering cartridge bus @10:58:26.43 Gate.setIR 1750,5000,0,6 @10:58:26.56 Gate.setIR 0,5000,0,6 @10:58:26.59 Loaded Archive Cartridge @10:58:26.60 Gate.power :cartridge,:OFF @10:58:46.07 -> Cmd.startFiltering @10:58:46.30 Duration of filtering limited to 1:05:00 @10:58:46.55 Priming sample loop w/100ml, bypass w/10ml @10:58:46.62 SP.setPosition! 0ml @10:58:46.66 Intake.open @10:58:46.69 Exhaust.open @10:58:47.55 Sea @-42.9psia @10:58:47.56 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @10:58:53.34 Vacuum tare is 24% of full scale @10:58:53.36 PB.open @10:58:53.40 Exhaust.close @10:58:54.19 Sea @-42.9psia @10:58:54.27 SP.reconfigure SPprime @10:58:54.34 SP.seek 10ml,1:21 @10:59:05.20 SP.setPosition! 0ml @10:59:05.24 PB.close @10:59:05.27 Exhaust.open @10:59:06.10 Sea @-42.9psia @10:59:06.17 SP.reconfigure SPprime @10:59:06.24 SP.seek 100ml,8:51 @11:00:59.98 Intake.close @11:01:00.73 Pressurizing cartridge to 26psi (try #1 of 3) @11:01:00.74 SP.setPosition! 0ml @11:01:00.79 PB.open @11:01:01.59 TV.seek :crack @11:01:02.38 TV.seek :clear @11:01:03.17 Intake.open @11:01:04.64 PB.close @11:01:05.35 Exhaust.close @11:01:06.14 Sea @-42.9psia @11:01:06.22 SP.reconfigure SPcharge @11:01:06.29 SP.seek 2.3ml,42 seconds @11:01:17.58 Cartridge pressurized with 0.598ml to 26.9psi @11:01:17.83 delay 3 seconds @11:01:20.89 Cartridge at 19.2psi 3 seconds later @11:01:20.97 TV.seek :cartridge @11:01:22.80 TV.seek :clear @11:01:24.62 Sampling 1000.0ml @11:01:24.63 SP.setPosition! 0ml @11:01:24.68 Exhaust.open @11:01:25.46 Sea @-42.9psia @11:01:25.55 SP.reconfigure SPsample @11:01:25.61 SP.seek 1000ml,1:23:53 @11:01:26.57 Cartridge 30 sampling 0/1000ml at 0.17ml/s with 7.8psi vacuum @11:02:26.58 Cartridge 30 sampling 16/1000ml at 0.22ml/s with 21.2psi vacuum @11:03:12.77 Flow averaged 0.24ml/s over 1:48 @11:03:12.79 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @11:03:13.71 Relieved excess vacuum in 1.1 seconds @11:03:13.74 SP.seek 1000ml,1:21:46 @11:03:18.58 Flow estimate reduced to 0.19ml/s @11:03:18.60 Intake.close @11:03:18.64 Exhaust.close @11:03:19.43 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:03:19.53 Sampled 26.3ml @11:03:19.61 TV.seek :bypass @11:03:21.22 TV.seek :clear @11:03:23.75 -> Cmd.startProcessing @11:03:24.00 PV.seek :reagent @11:03:24.85 Sample loop closed. Ready to Deliver Reagents @11:03:24.86 ArS.seek :sealed @11:03:29.13 ArS.seek :evac @11:03:43.05 delay 20 seconds @11:04:03.09 ArS.seek :clear @11:04:20.80 RNAlater soak for 10:00 @11:14:20.82 ArS.seek :tVent @11:14:23.39 delay 1 second @11:14:24.67 ArS.seek :evac @11:14:40.29 delay 10 seconds @11:14:50.33 Sample preserved and evacuated @11:14:51.46 -> Cmd.stop @11:14:51.60 PV.seek :locked @11:14:52.49 PinPort.seek :clear @11:14:52.64 ArS.seek :clear @11:14:52.83 LyS2.seek :clear @11:14:52.97 AirS.seek :clear @11:15:10.40 ArS.coast @11:15:10.45 LyS1.coast @11:15:10.50 LyS2.coast @11:15:10.55 AirS.coast @11:15:10.60 TV.coast @11:15:10.65 SP.coast @11:15:10.70 SC.coast @11:15:10.75 PV.coast @11:15:10.80 PinPort.coast @11:15:10.98 Gate.power :motor,:OFF @11:15:10.99 Gate.power :main,:OFF @11:15:11.02 Gate.power :core,:OFF @11:15:11.04 Gate.power :analytic1,:OFF @11:15:11.06 Gate.power :analytic2,:OFF @11:15:11.08 Gate.power :analytic3,:OFF @11:15:11.10 Safely stopped and ready to power off ##-- file closed 2018-08-29T15:26:07.266Z ##-- file opened 2018-08-29T15:26:07.273Z @11:25:59.25 -> showStatus @11:26:01.22 -> Cmd.loadCartridge @11:26:01.35 Gate.power :main,:ON,limit to 5000mA @11:26:01.36 Gate.power :analytic1,:ON,limit to 5000mA @11:26:01.39 Gate.power :analytic2,:ON,limit to 5000mA @11:26:01.41 Gate.power :analytic3,:ON,limit to 5000mA @11:26:01.55 Powering core bus @11:26:03.86 SPR Module 1, FW Rev. B, FPGA Rev. C @11:26:03.95 ArS.configure ArSconfig @11:26:04.05 LyS1.configure LyS1config @11:26:04.17 LyS2.configure LyS2config @11:26:04.27 AirS.configure AirSconfig @11:26:04.39 TV.configure TVconfig @11:26:04.50 SP.configure SPconfig @11:26:04.62 SC.configure SCconfig @11:26:04.74 PV.configure PVconfig @11:26:04.85 PinPort.configure PPconfig @11:26:04.97 Sea.configure SPconfig @11:26:05.03 All elf controllers running firmware version 4.5 @11:26:05.05 Gate.power :motor,:ON,limit to 5000mA @11:26:05.20 ArS.reconfigure ArSconfig @11:26:05.47 ArS.home.jog 0 @11:26:05.62 PV.reconfigure PVconfig @11:26:05.68 TV.reconfigure TVconfig @11:26:05.71 LyS2.reconfigure LyS2config @11:26:05.74 LyS1.reconfigure LyS1config @11:26:05.79 PinPort.reconfigure PPconfig @11:26:05.90 AirS.reconfigure AirSconfig @11:26:06.06 PV.home.jog 0 @11:26:06.13 LyS2.home.jog 0 @11:26:06.17 PinPort.home.jog 0 @11:26:06.21 TV.home.jog 0 @11:26:06.24 LyS1.home.jog 0 @11:26:06.29 AirS.home.jog 0 @11:26:06.57 ArS.reconfigure HomeMagSyringe @11:26:06.82 PV.reconfigure HomePV @11:26:06.85 TV.reconfigure HomeTV @11:26:06.90 PinPort.reconfigure HomePP @11:26:06.97 LyS2.reconfigure HomeSyringe @11:26:07.01 ArS.jog -225 @11:26:07.16 TV.jog -534 @11:26:07.22 PV.jog -1477 @11:26:07.25 PinPort.jog -111 @11:26:07.28 LyS2.jog -257 @11:26:07.49 LyS1.reconfigure HomeSyringe @11:26:07.50 AirS.reconfigure HomeMagSyringe @11:26:07.59 LyS1.jog -263 @11:26:07.62 AirS.jog -320 @11:26:07.76 PinPort.setRawPosition! 0 @11:26:07.77 ArS.setRawPosition! -50 @11:26:07.84 PinPort.seek :home @11:26:08.10 ArS.seek :home @11:26:08.28 AirS.setRawPosition! -50 @11:26:08.31 LyS1.setRawPosition! -50 @11:26:08.34 PinPort.reconfigure PPconfig @11:26:08.36 LyS2.setRawPosition! -50 @11:26:08.52 AirS.seek :home @11:26:08.56 LyS1.seek :home @11:26:08.59 LyS2.seek :home @11:26:08.81 ArS.reconfigure ArSconfig @11:26:08.82 PV.setRawPosition! -50 @11:26:08.91 PV.seek :home @11:26:09.04 AirS.reconfigure AirSconfig @11:26:09.06 LyS2.reconfigure LyS2config @11:26:09.09 LyS1.reconfigure LyS1config @11:26:09.33 TV.setRawPosition! 0 @11:26:09.35 PV.reconfigure PVconfig @11:26:09.43 TV.seek :home @11:26:09.56 TV.reconfigure TVconfig @11:26:09.75 TV.seek :clear @11:26:11.36 TV.coast @11:26:11.42 SC.reconfigure HomeSC @11:26:11.48 SC.home.jog 0 @11:26:12.22 SC.reconfigure SCconfig @11:26:12.28 SC.seek 30 @11:26:12.39 Selecting Cartridge 29 @11:26:12.69 SC.seek 29 @11:26:13.19 LyS2.seek :locked @11:26:13.31 PV.seek :locked @11:26:13.45 AirS.seek :locked @11:26:17.53 Powering cartridge bus @11:26:19.66 Gate.setIR 1750,5000,0,6 @11:26:19.78 Gate.setIR 0,5000,0,6 @11:26:19.83 Loaded Lyse-n-Go Cartridge @11:26:19.84 PuckHeater.configure HeatConfig @11:26:19.89 Gate.power :heat,:ON,limit to 5000mA @11:26:39.96 -> Cmd.startFiltering @11:26:40.20 Duration of filtering limited to 1:05:00 @11:26:40.46 Priming sample loop w/100ml, bypass w/10ml @11:26:40.53 SP.setPosition! 0ml @11:26:40.57 Intake.open @11:26:40.59 Exhaust.open @11:26:41.41 Sea @-42.9psia @11:26:41.43 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @11:26:47.21 Vacuum tare is 24% of full scale @11:26:47.22 PB.open @11:26:47.27 Exhaust.close @11:26:48.05 Sea @-42.9psia @11:26:48.14 SP.reconfigure SPprime @11:26:48.20 SP.seek 10ml,1:21 @11:26:59.05 SP.setPosition! 0ml @11:26:59.10 PB.close @11:26:59.12 Exhaust.open @11:26:59.96 Sea @-42.9psia @11:27:00.05 SP.reconfigure SPprime @11:27:00.12 SP.seek 100ml,8:51 @11:28:53.79 Intake.close @11:28:54.49 Pressurizing cartridge to 26psi (try #1 of 3) @11:28:54.51 SP.setPosition! 0ml @11:28:54.56 PB.open @11:28:55.37 TV.seek :crack @11:28:56.16 TV.seek :clear @11:28:56.95 Intake.open @11:28:58.39 PB.close @11:28:59.13 Exhaust.close @11:28:59.92 Sea @-42.9psia @11:29:00.01 SP.reconfigure SPcharge @11:29:00.07 SP.seek 2.3ml,42 seconds @11:29:08.49 Cartridge pressurized with 0.329ml to 26.3psi @11:29:08.87 delay 3 seconds @11:29:11.94 Cartridge at 20.0psi 3 seconds later @11:29:12.02 TV.seek :cartridge @11:29:13.85 TV.seek :clear @11:29:15.67 Sampling 1000.0ml @11:29:15.68 SP.setPosition! 0ml @11:29:15.72 Exhaust.open @11:29:16.51 Sea @-42.9psia @11:29:16.60 SP.reconfigure SPsample @11:29:16.67 SP.seek 1000ml,1:23:53 @11:29:17.63 Cartridge 29 sampling 0/1000ml at 0.17ml/s with 4.9psi vacuum @11:30:17.63 Cartridge 29 sampling 33/1000ml at 0.53ml/s with 24.7psi vacuum @11:31:17.62 Cartridge 29 sampling 66/1000ml at 0.56ml/s with 22.9psi vacuum @11:32:17.63 Cartridge 29 sampling 99/1000ml at 0.56ml/s with 23.6psi vacuum @11:33:17.64 Cartridge 29 sampling 131/1000ml at 0.52ml/s with 20.9psi vacuum @11:34:17.63 Cartridge 29 sampling 162/1000ml at 0.50ml/s with 19.6psi vacuum @11:35:17.63 Cartridge 29 sampling 193/1000ml at 0.52ml/s with 22.5psi vacuum @11:36:17.62 Cartridge 29 sampling 224/1000ml at 0.49ml/s with 23.4psi vacuum @11:37:17.63 Cartridge 29 sampling 253/1000ml at 0.49ml/s with 23.7psi vacuum @11:38:17.63 Cartridge 29 sampling 282/1000ml at 0.45ml/s with 19.4psi vacuum @11:39:17.63 Cartridge 29 sampling 309/1000ml at 0.42ml/s with 23.3psi vacuum @11:40:17.64 Cartridge 29 sampling 335/1000ml at 0.40ml/s with 23.3psi vacuum @11:41:17.63 Cartridge 29 sampling 360/1000ml at 0.39ml/s with 26.7psi vacuum @11:42:17.63 Cartridge 29 sampling 384/1000ml at 0.37ml/s with 24.8psi vacuum @11:43:17.89 Cartridge 29 sampling 405/1000ml at 0.32ml/s with 26.9psi vacuum @11:44:17.64 Cartridge 29 sampling 425/1000ml at 0.27ml/s with 24.3psi vacuum @11:45:17.64 Cartridge 29 sampling 443/1000ml at 0.28ml/s with 25.8psi vacuum @11:46:17.64 Cartridge 29 sampling 459/1000ml at 0.23ml/s with 22.3psi vacuum @11:47:17.64 Cartridge 29 sampling 474/1000ml at 0.25ml/s with 18.7psi vacuum @11:48:17.64 Cartridge 29 sampling 488/1000ml at 0.21ml/s with 23.7psi vacuum @11:49:17.64 Cartridge 29 sampling 501/1000ml at 0.20ml/s with 23.0psi vacuum @11:50:17.64 Cartridge 29 sampling 514/1000ml at 0.20ml/s with 25.2psi vacuum @11:51:17.64 Cartridge 29 sampling 526/1000ml at 0.20ml/s with 23.9psi vacuum @11:52:17.64 Cartridge 29 sampling 538/1000ml at 0.20ml/s with 26.4psi vacuum @11:53:06.82 Flow averaged 0.39ml/s over 23:42 @11:53:06.85 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @11:53:07.47 Relieved excess vacuum in 9.7 seconds @11:53:07.50 SP.seek 1000ml,38:11 @11:53:17.64 Cartridge 29 sampling 550/1000ml at 0.20ml/s with 24.1psi vacuum @11:53:42.49 Flow averaged 0.23ml/s over 31 seconds @11:53:42.51 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi @11:53:43.14 Relieved excess vacuum in 5.4 seconds @11:53:43.17 SP.seek 1000ml,37:36 @11:53:58.04 Flow averaged 0.22ml/s over 14 seconds @11:53:58.07 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @11:53:58.68 Relieved excess vacuum in 2.4 seconds @11:53:58.71 SP.seek 1000ml,37:22 @11:54:17.64 Cartridge 29 sampling 562/1000ml at 0.20ml/s with 26.5psi vacuum @11:54:18.44 Flow averaged 0.20ml/s over 19 seconds @11:54:18.48 Waiting up to 14 seconds for vacuum to reduce from 28.6 to 18.0psi @11:54:19.10 Relieved excess vacuum in 1.4 seconds @11:54:19.13 SP.seek 1000ml,37:03 @11:54:32.52 Flow averaged 0.20ml/s over 14 seconds @11:54:32.54 Intake.close @11:54:32.59 Exhaust.close @11:54:33.37 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:54:33.47 Sampled 564.3ml @11:54:33.55 TV.seek :bypass @11:54:35.17 TV.seek :clear @11:54:37.43 -> Cmd.startProcessing @11:54:39.23 SPR.command \"read_sequences\" @11:54:39.49 SPR.command \"stats_on\" @11:54:39.53 SPR.command \"start 0 1\" @11:54:41.53 Begun log /var/log/esp/spr18aug2911h.spr @11:54:42.34 Handoff.select :waste @11:54:42.46 ArS.seek empty @11:55:00.20 PinPort.seek :cartridge @11:55:05.91 LyS1.seek 1350ul @11:55:11.86 PV.seek :reagent @11:55:12.70 AirS.seek :tVent @11:55:14.76 delay 2 seconds @11:55:16.79 AirS.reconfigure EvacAirS @11:55:16.87 AirS.seek :evac @11:55:39.18 delay 15 seconds @11:55:54.20 AirS.reconfigure AirSconfig @11:55:54.26 AirS.seek :tVent @11:56:08.48 delay 2 seconds @11:56:10.50 AirS.reconfigure EvacAirS @11:56:10.56 AirS.seek :evac @11:56:32.85 delay 15 seconds @11:56:47.88 AirS.reconfigure AirSconfig @11:56:47.94 AirS.seek :tVent @11:57:02.14 delay 2 seconds @11:57:04.17 AirS.reconfigure EvacAirS @11:57:04.23 AirS.seek :evac @11:57:26.51 delay 15 seconds @11:57:41.54 AirS.reconfigure AirSconfig @11:57:41.62 AirS.seek :tVent @11:57:55.82 delay 2 seconds @11:57:57.85 AirS.reconfigure EvacAirS @11:57:57.90 AirS.seek :evac @11:58:20.19 delay 15 seconds @11:58:35.22 AirS.reconfigure AirSconfig @11:58:35.28 AirS.seek :tVent @11:58:49.51 delay 2 seconds @11:58:51.54 AirS.reconfigure EvacAirS @11:58:51.62 AirS.seek :evac @11:59:13.92 delay 15 seconds @11:59:28.95 delay 1:30 @12:00:58.97 AirS.reconfigure AirSconfig @12:00:59.03 AirS.seek :fVent @12:01:00.46 delay 15 seconds @12:01:15.49 LyS1.reconfigure SlowLyS1 @12:01:15.59 LyS1.seek 1050ul @12:01:45.85 delay 5 seconds @12:01:50.87 Handoff.select :blocked @12:01:51.04 LyS1.seek 450ul @12:02:51.00 delay 10 seconds @12:03:01.03 AirS.reconfigure SlowAirS @12:03:01.13 AirS.reconfigure to mix into 1.37806 parts LyS1 @12:03:01.50 AirS.seek evac @12:03:01.55 LyS1.seek 300ul @12:03:16.63 AirS.reconfigure AirSconfig @12:03:16.69 AirS.seek :full @12:03:30.91 LyS1.seek :empty @12:04:00.99 PuckHeater.seek 84.1C @12:05:02.09 PuckHeater.hold 15:00,84.1C @12:19:56.20 LyS1.reconfigure LyS1config @12:19:56.27 LyS1.seek 900ul @12:20:04.61 AirS.seek :tVent @12:20:04.71 delay 2 seconds @12:20:06.73 AirS.reconfigure HalfSpeedAirS @12:20:06.78 AirS.seek :evac @12:20:34.52 delay 15 seconds @12:20:49.55 AirS.reconfigure AirSconfig @12:20:49.61 AirS.seek :tVent @12:21:03.81 delay 2 seconds @12:21:05.84 AirS.reconfigure HalfSpeedAirS @12:21:05.89 AirS.seek :evac @12:21:33.62 delay 15 seconds @12:21:48.65 AirS.reconfigure AirSconfig @12:21:48.73 AirS.seek :tVent @12:22:02.94 delay 2 seconds @12:22:04.97 AirS.reconfigure HalfSpeedAirS @12:22:05.03 AirS.seek :evac @12:22:32.77 delay 15 seconds @12:22:47.79 delay 1:00 @12:23:47.80 Handoff.select :waste @12:23:47.91 delay 15 seconds @12:24:02.93 AirS.seek :fVent @12:24:05.12 delay 10 seconds @12:24:15.15 AirS.reconfigure AirSconfig @12:24:15.21 AirS.seek :evac @12:24:16.64 Lysis Recovered in LyS1 @12:24:16.65 Lysis Complete @12:24:16.66 Handoff.select :waste @12:24:16.69 LyS2.seek :full @12:24:16.78 LyS1.reconfigure DAmixLyS1 @12:24:16.84 LyS2.reconfigure DAmixLyS2 @12:24:17.23 LyS1.seek 650ul @12:24:17.27 LyS2.seek 1480ul @12:25:04.58 delay 5 seconds @12:25:09.60 Handoff.select :SPR @12:25:09.72 SPR.command \"start 2 14\" @12:25:10.64 Running Experiment 2 @12:25:10.65 delay 10 seconds @12:25:20.67 SPR.command \"start 2 0\" @12:25:24.00 Waiting up to 11:00 for SPR to enter state 1 @12:32:42.29 SPR.command \"hold_on\" @12:32:44.47 LyS1.seek 300ul @12:32:44.51 LyS2.seek 1130ul @12:33:50.67 delay 15 seconds @12:34:05.69 SPR.command \"hold_off\" @12:34:08.93 Waiting up to 25:00 for SPR to enter state 7 @12:45:44.48 Handoff.select :waste @12:45:44.61 LyS1.reconfigure LyS1config @12:45:44.67 LyS2.reconfigure LyS2config @12:45:44.73 Rep #1 SPR Analysis has Completed @12:45:45.41 Ended log /var/log/esp/spr18aug2911h.spr @12:45:45.43 SPR.command \"stats_off\" @12:45:47.59 -> Cmd.stop @12:45:47.70 Handoff.select :close @12:45:47.90 Gate.power :heat,:OFF @12:45:47.93 Gate.power :cartridge,:OFF @12:45:47.99 PV.seek :locked @12:45:48.88 PinPort.seek :clear @12:45:54.88 ArS.seek :clear @12:45:55.01 LyS1.seek :clear @12:45:55.14 LyS2.seek :clear @12:45:55.27 AirS.seek :clear @12:46:12.63 ArS.coast @12:46:12.68 LyS1.coast @12:46:12.73 LyS2.coast @12:46:12.78 AirS.coast @12:46:12.83 TV.coast @12:46:12.88 SP.coast @12:46:12.93 SC.coast @12:46:12.98 PV.coast @12:46:13.03 PinPort.coast @12:46:13.22 Gate.power :motor,:OFF @12:46:13.23 Gate.power :main,:OFF @12:46:13.25 Gate.power :core,:OFF @12:46:13.27 Gate.power :analytic1,:OFF @12:46:13.29 Gate.power :analytic2,:OFF @12:46:13.31 Gate.power :analytic3,:OFF @12:46:13.33 Safely stopped and ready to power off ##-- file closed 2018-08-29T16:58:35.349Z ##-- file opened 2018-08-29T16:58:35.358Z @12:58:18.81 -> showStatus @12:58:20.79 -> Cmd.loadCartridge @12:58:20.92 Gate.power :main,:ON,limit to 5000mA @12:58:20.93 Gate.power :analytic1,:ON,limit to 5000mA @12:58:20.96 Gate.power :analytic2,:ON,limit to 5000mA @12:58:20.97 Gate.power :analytic3,:ON,limit to 5000mA @12:58:21.12 Powering core bus @12:58:23.42 SPR Module 1, FW Rev. B, FPGA Rev. C @12:58:23.51 ArS.configure ArSconfig @12:58:23.61 LyS1.configure LyS1config @12:58:23.73 LyS2.configure LyS2config @12:58:23.83 AirS.configure AirSconfig @12:58:23.94 TV.configure TVconfig @12:58:24.06 SP.configure SPconfig @12:58:24.18 SC.configure SCconfig @12:58:24.30 PV.configure PVconfig @12:58:24.42 PinPort.configure PPconfig @12:58:24.54 Sea.configure SPconfig @12:58:24.60 All elf controllers running firmware version 4.5 @12:58:24.62 Gate.power :motor,:ON,limit to 5000mA @12:58:24.75 ArS.reconfigure ArSconfig @12:58:25.00 ArS.home.jog 0 @12:58:25.20 PinPort.reconfigure PPconfig @12:58:25.27 PV.reconfigure PVconfig @12:58:25.31 AirS.reconfigure AirSconfig @12:58:25.34 LyS2.reconfigure LyS2config @12:58:25.37 LyS1.reconfigure LyS1config @12:58:25.48 TV.reconfigure TVconfig @12:58:25.60 PinPort.home.jog 0 @12:58:25.64 AirS.home.jog 0 @12:58:25.68 PV.home.jog 0 @12:58:25.71 LyS1.home.jog 0 @12:58:25.74 LyS2.home.jog 0 @12:58:25.82 TV.home.jog 0 @12:58:26.14 ArS.reconfigure HomeMagSyringe @12:58:26.37 ArS.jog -236 @12:58:26.54 AirS.reconfigure HomeMagSyringe @12:58:26.56 LyS2.reconfigure HomeSyringe @12:58:26.59 PinPort.reconfigure HomePP @12:58:26.64 PV.reconfigure HomePV @12:58:26.67 TV.reconfigure HomeTV @12:58:26.71 LyS1.reconfigure HomeSyringe @12:58:26.95 LyS2.jog -260 @12:58:27.00 AirS.jog -325 @12:58:27.03 PinPort.jog -112 @12:58:27.06 PV.jog -1478 @12:58:27.09 TV.jog -539 @12:58:27.12 LyS1.jog -266 @12:58:27.36 ArS.setRawPosition! -50 @12:58:27.41 ArS.seek :home @12:58:27.86 AirS.setRawPosition! -50 @12:58:27.90 LyS1.setRawPosition! -50 @12:58:27.92 ArS.reconfigure ArSconfig @12:58:27.94 LyS2.setRawPosition! -50 @12:58:27.97 PinPort.setRawPosition! 0 @12:58:28.13 AirS.seek :home @12:58:28.16 LyS1.seek :home @12:58:28.24 LyS2.seek :home @12:58:28.43 PinPort.seek :home @12:58:28.61 PV.setRawPosition! -50 @12:58:28.64 PinPort.reconfigure PPconfig @12:58:28.72 AirS.reconfigure AirSconfig @12:58:28.76 PV.seek :home @12:58:28.92 LyS1.reconfigure LyS1config @12:58:28.94 LyS2.reconfigure LyS2config @12:58:29.11 TV.setRawPosition! 0 @12:58:29.17 TV.seek :home @12:58:29.27 PV.reconfigure PVconfig @12:58:29.29 TV.reconfigure TVconfig @12:58:29.50 TV.seek :clear @12:58:31.13 TV.coast @12:58:31.19 SC.reconfigure HomeSC @12:58:31.25 SC.home.jog 0 @12:58:32.01 SC.reconfigure SCconfig @12:58:32.07 SC.seek 29 @12:58:32.17 Selecting Cartridge 28 @12:58:32.47 SC.seek 28 @12:58:32.97 LyS2.seek :locked @12:58:33.10 PV.seek :locked @12:58:33.24 AirS.seek :locked @12:58:37.32 Powering cartridge bus @12:58:39.44 Gate.setIR 1750,5000,0,6 @12:58:39.56 Gate.setIR 0,5000,0,6 @12:58:39.60 Loaded Archive Cartridge @12:58:39.61 Gate.power :cartridge,:OFF @12:58:59.16 -> Cmd.startFiltering @12:58:59.38 Duration of filtering limited to 1:05:00 @12:58:59.63 Priming sample loop w/100ml, bypass w/10ml @12:58:59.71 SP.setPosition! 0ml @12:58:59.75 Intake.open @12:58:59.81 Exhaust.open @12:59:00.58 Sea @-42.9psia @12:59:00.60 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @12:59:06.38 Vacuum tare is 25% of full scale @12:59:06.39 PB.open @12:59:06.44 Exhaust.close @12:59:07.22 Sea @-42.9psia @12:59:07.31 SP.reconfigure SPprime @12:59:07.37 SP.seek 10ml,1:21 @12:59:18.22 SP.setPosition! 0ml @12:59:18.27 PB.close @12:59:18.29 Exhaust.open @12:59:19.08 Sea @-42.9psia @12:59:19.16 SP.reconfigure SPprime @12:59:19.22 SP.seek 100ml,8:51 @13:01:12.87 Intake.close @13:01:13.61 Pressurizing cartridge to 26psi (try #1 of 3) @13:01:13.62 SP.setPosition! 0ml @13:01:13.67 PB.open @13:01:14.45 TV.seek :crack @13:01:15.24 TV.seek :clear @13:01:16.04 Intake.open @13:01:17.50 PB.close @13:01:18.26 Exhaust.close @13:01:19.00 Sea @-42.9psia @13:01:19.09 SP.reconfigure SPcharge @13:01:19.15 SP.seek 2.3ml,42 seconds @13:01:26.49 Cartridge pressurized with 0.250ml to 26.2psi @13:01:26.73 delay 3 seconds @13:01:29.80 Cartridge at 22.4psi 3 seconds later @13:01:29.88 TV.seek :cartridge @13:01:31.71 TV.seek :clear @13:01:33.54 Sampling 1000.0ml @13:01:33.56 SP.setPosition! 0ml @13:01:33.60 Exhaust.open @13:01:34.34 Sea @-42.9psia @13:01:34.43 SP.reconfigure SPsample @13:01:34.49 SP.seek 1000ml,1:23:53 @13:01:35.45 Cartridge 28 sampling 0/1000ml at 0.17ml/s with 4.9psi vacuum @13:02:35.46 Cartridge 28 sampling 23/1000ml at 0.38ml/s with 25.9psi vacuum @13:03:35.47 Cartridge 28 sampling 43/1000ml at 0.29ml/s with 18.8psi vacuum @13:04:35.46 Cartridge 28 sampling 60/1000ml at 0.22ml/s with 24.8psi vacuum @13:05:35.46 Cartridge 28 sampling 75/1000ml at 0.25ml/s with 25.1psi vacuum @13:06:35.46 Cartridge 28 sampling 88/1000ml at 0.20ml/s with 29.3psi vacuum @13:06:59.50 Flow averaged 0.29ml/s over 5:22 @13:06:59.52 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @13:07:00.15 Relieved excess vacuum in 4.6 seconds @13:07:00.18 SP.seek 1000ml,1:16:11 @13:07:14.94 Flow averaged 0.22ml/s over 14 seconds @13:07:14.96 Waiting up to 15 seconds for vacuum to reduce from 28.9 to 18.0psi @13:07:15.58 Relieved excess vacuum in 2.3 seconds @13:07:15.61 SP.seek 1000ml,1:15:56 @13:07:22.28 Flow estimate reduced to 0.18ml/s @13:07:22.30 Intake.close @13:07:22.33 Exhaust.close @13:07:23.11 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @13:07:23.21 Sampled 96.5ml @13:07:23.29 TV.seek :bypass @13:07:24.90 TV.seek :clear @13:07:27.58 -> Cmd.startProcessing @13:07:27.84 PV.seek :reagent @13:07:28.69 Sample loop closed. Ready to Deliver Reagents @13:07:28.71 ArS.seek :sealed @13:07:32.97 ArS.seek :evac @13:07:46.91 delay 20 seconds @13:08:06.95 ArS.seek :clear @13:08:24.66 RNAlater soak for 10:00 @13:18:24.69 ArS.seek :tVent @13:18:27.26 delay 1 second @13:18:28.29 ArS.seek :evac @13:18:43.91 delay 10 seconds @13:18:53.95 Sample preserved and evacuated @13:18:54.93 -> Cmd.stop @13:18:55.08 PV.seek :locked @13:18:55.97 PinPort.seek :clear @13:18:56.10 ArS.seek :clear @13:18:56.29 LyS2.seek :clear @13:18:56.43 AirS.seek :clear @13:19:13.86 ArS.coast @13:19:13.91 LyS1.coast @13:19:13.96 LyS2.coast @13:19:14.01 AirS.coast @13:19:14.06 TV.coast @13:19:14.11 SP.coast @13:19:14.16 SC.coast @13:19:14.21 PV.coast @13:19:14.26 PinPort.coast @13:19:14.45 Gate.power :motor,:OFF @13:19:14.47 Gate.power :main,:OFF @13:19:14.49 Gate.power :core,:OFF @13:19:14.51 Gate.power :analytic1,:OFF @13:19:14.53 Gate.power :analytic2,:OFF @13:19:14.55 Gate.power :analytic3,:OFF @13:19:14.57 Safely stopped and ready to power off ##-- file closed 2018-08-30T00:25:18.954Z