##-- file opened 2018-08-29T05:11:47.440Z @01:10:44.69 -> showStatus @01:10:46.30 -> Cmd.loadCartridge @01:10:46.42 Gate.power :main,:ON,limit to 5000mA @01:10:46.44 Gate.power :analytic1,:ON,limit to 5000mA @01:10:46.46 Gate.power :analytic2,:ON,limit to 5000mA @01:10:46.48 Gate.power :analytic3,:ON,limit to 5000mA @01:10:46.62 Powering core bus @01:10:48.93 SPR Module 1, FW Rev. B, FPGA Rev. C @01:10:49.03 ArS.configure ArSconfig @01:10:49.12 LyS1.configure LyS1config @01:10:49.24 LyS2.configure LyS2config @01:10:49.34 AirS.configure AirSconfig @01:10:49.46 TV.configure TVconfig @01:10:49.58 SP.configure SPconfig @01:10:49.70 SC.configure SCconfig @01:10:49.82 PV.configure PVconfig @01:10:49.94 PinPort.configure PPconfig @01:10:50.06 Sea.configure SPconfig @01:10:50.11 All elf controllers running firmware version 4.5 @01:10:50.13 Gate.power :motor,:ON,limit to 5000mA @01:10:50.30 ArS.reconfigure ArSconfig @01:10:50.53 ArS.home.jog 0 @01:10:50.69 PV.reconfigure PVconfig @01:10:50.76 TV.reconfigure TVconfig @01:10:50.79 LyS2.reconfigure LyS2config @01:10:50.84 PinPort.reconfigure PPconfig @01:10:50.90 LyS1.reconfigure LyS1config @01:10:50.93 AirS.reconfigure AirSconfig @01:10:51.13 TV.home.jog 0 @01:10:51.16 PV.home.jog 0 @01:10:51.19 PinPort.home.jog 0 @01:10:51.22 LyS2.home.jog 0 @01:10:51.24 LyS1.home.jog 0 @01:10:51.27 AirS.home.jog 0 @01:10:51.60 ArS.reconfigure HomeMagSyringe @01:10:51.81 PV.reconfigure HomePV @01:10:51.84 TV.reconfigure HomeTV @01:10:51.98 PinPort.reconfigure HomePP @01:10:52.04 ArS.jog -226 @01:10:52.21 PinPort.jog -111 @01:10:52.24 PV.jog -1472 @01:10:52.27 TV.jog -532 @01:10:52.48 LyS2.reconfigure HomeSyringe @01:10:52.51 LyS1.reconfigure HomeSyringe @01:10:52.54 AirS.reconfigure HomeMagSyringe @01:10:52.66 LyS2.jog -261 @01:10:52.71 LyS1.jog -258 @01:10:52.74 AirS.jog -310 @01:10:52.90 PinPort.setRawPosition! 0 @01:10:52.92 ArS.setRawPosition! -50 @01:10:53.01 ArS.seek :home @01:10:53.29 PinPort.seek :home @01:10:53.44 PinPort.reconfigure PPconfig @01:10:53.48 LyS2.setRawPosition! -50 @01:10:53.50 LyS1.setRawPosition! -50 @01:10:53.53 AirS.setRawPosition! -50 @01:10:53.64 LyS2.seek :home @01:10:53.77 AirS.seek :home @01:10:53.80 LyS1.seek :home @01:10:53.92 PV.setRawPosition! -50 @01:10:53.95 ArS.reconfigure ArSconfig @01:10:54.03 PV.seek :home @01:10:54.19 AirS.reconfigure AirSconfig @01:10:54.23 LyS2.reconfigure LyS2config @01:10:54.26 TV.setRawPosition! 0 @01:10:54.29 LyS1.reconfigure LyS1config @01:10:54.45 TV.seek :home @01:10:54.59 TV.reconfigure TVconfig @01:10:54.61 PV.reconfigure PVconfig @01:10:54.97 TV.seek :clear @01:10:56.59 TV.coast @01:10:56.64 SC.reconfigure HomeSC @01:10:56.70 SC.home.jog 0 @01:10:57.46 SC.reconfigure SCconfig @01:10:57.52 SC.seek 34 @01:10:57.64 Selecting Cartridge 33 @01:10:57.94 SC.seek 33 @01:10:58.44 LyS2.seek :locked @01:10:58.57 PV.seek :locked @01:10:58.70 AirS.seek :locked @01:11:02.78 Powering cartridge bus @01:11:04.90 Gate.setIR 1750,5000,0,6 @01:11:05.03 Gate.setIR 0,5000,0,6 @01:11:05.06 Loaded Archive Cartridge @01:11:05.07 Gate.power :cartridge,:OFF @01:11:24.63 -> Cmd.startFiltering @01:11:24.85 Duration of filtering limited to 1:05:00 @01:11:25.10 Priming sample loop w/100ml, bypass w/10ml @01:11:25.17 SP.setPosition! 0ml @01:11:25.22 Intake.open @01:11:25.28 Exhaust.open @01:11:26.12 Sea @-42.9psia @01:11:26.13 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @01:11:31.91 Vacuum tare is 24% of full scale @01:11:31.93 PB.open @01:11:31.98 Exhaust.close @01:11:32.79 Sea @-42.9psia @01:11:32.87 SP.reconfigure SPprime @01:11:32.94 SP.seek 10ml,1:21 @01:11:43.79 SP.setPosition! 0ml @01:11:43.83 PB.close @01:11:43.86 Exhaust.open @01:11:44.69 Sea @-42.9psia @01:11:44.77 SP.reconfigure SPprime @01:11:44.83 SP.seek 100ml,8:51 @01:13:32.79 Intake.close @01:13:33.52 Pressurizing cartridge to 26psi (try #1 of 3) @01:13:33.53 SP.setPosition! 0ml @01:13:33.58 PB.open @01:13:34.40 TV.seek :crack @01:13:35.19 TV.seek :clear @01:13:35.99 Intake.open @01:13:37.44 PB.close @01:13:38.19 Exhaust.close @01:13:38.98 Sea @-42.9psia @01:13:39.06 SP.reconfigure SPcharge @01:13:39.12 SP.seek 2.3ml,42 seconds @01:13:47.14 Cartridge pressurized with 0.298ml to 26.0psi @01:13:47.51 Pressure peaked at 26.2psi @01:13:47.53 delay 3 seconds @01:13:50.61 Cartridge at 23.5psi 3 seconds later @01:13:50.69 TV.seek :cartridge @01:13:52.50 TV.seek :clear @01:13:54.31 Sampling 1000.0ml @01:13:54.32 SP.setPosition! 0ml @01:13:54.37 Exhaust.open @01:13:55.16 Sea @-42.9psia @01:13:55.25 SP.reconfigure SPsample @01:13:55.31 SP.seek 1000ml,1:23:53 @01:13:56.27 Cartridge 33 sampling 0/1000ml at 0.17ml/s with 4.9psi vacuum @01:14:56.28 Cartridge 33 sampling 26/1000ml at 0.37ml/s with 25.3psi vacuum @01:15:56.28 Cartridge 33 sampling 50/1000ml at 0.38ml/s with 28.2psi vacuum @01:16:56.28 Cartridge 33 sampling 72/1000ml at 0.38ml/s with 25.8psi vacuum @01:17:56.28 Cartridge 33 sampling 93/1000ml at 0.32ml/s with 27.9psi vacuum @01:18:56.28 Cartridge 33 sampling 112/1000ml at 0.29ml/s with 27.2psi vacuum @01:19:56.28 Cartridge 33 sampling 129/1000ml at 0.31ml/s with 25.5psi vacuum @01:20:56.28 Cartridge 33 sampling 144/1000ml at 0.28ml/s with 23.0psi vacuum @01:21:56.28 Cartridge 33 sampling 157/1000ml at 0.25ml/s with 23.7psi vacuum @01:22:56.28 Cartridge 33 sampling 170/1000ml at 0.20ml/s with 25.3psi vacuum @01:23:56.28 Cartridge 33 sampling 182/1000ml at 0.20ml/s with 26.4psi vacuum @01:24:06.65 Flow averaged 0.30ml/s over 10:12 @01:24:06.67 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi @01:24:07.30 Relieved excess vacuum in .9 seconds @01:24:07.32 SP.seek 1000ml,1:08:33 @01:24:22.20 Flow averaged 0.22ml/s over 14 seconds @01:24:22.22 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @01:24:23.11 Relieved excess vacuum in 2.4 seconds @01:24:23.13 SP.seek 1000ml,1:08:19 @01:24:37.98 Flow averaged 0.22ml/s over 14 seconds @01:24:37.99 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @01:24:38.63 Relieved excess vacuum in 2.4 seconds @01:24:38.65 SP.seek 1000ml,1:08:05 @01:24:43.94 Flow estimate reduced to 0.18ml/s @01:24:43.96 Intake.close @01:24:44.02 Exhaust.close @01:24:44.80 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @01:24:44.90 Sampled 190.5ml @01:24:44.98 TV.seek :bypass @01:24:46.59 TV.seek :clear @01:24:49.04 -> Cmd.startProcessing @01:24:49.29 PV.seek :reagent @01:24:50.15 Sample loop closed. Ready to Deliver Reagents @01:24:50.16 ArS.seek :sealed @01:24:54.43 ArS.seek :evac @01:25:08.36 delay 20 seconds @01:25:28.39 ArS.seek :clear @01:25:46.10 RNAlater soak for 10:00 @01:35:46.14 ArS.seek :tVent @01:35:48.71 delay 1 second @01:35:49.73 ArS.seek :evac @01:36:05.35 delay 10 seconds @01:36:15.38 Sample preserved and evacuated @01:36:16.38 -> Cmd.stop @01:36:16.52 PV.seek :locked @01:36:17.42 PinPort.seek :clear @01:36:17.56 ArS.seek :clear @01:36:17.74 LyS2.seek :clear @01:36:17.86 AirS.seek :clear @01:36:35.30 ArS.coast @01:36:35.36 LyS1.coast @01:36:35.41 LyS2.coast @01:36:35.45 AirS.coast @01:36:35.50 TV.coast @01:36:35.55 SP.coast @01:36:35.60 SC.coast @01:36:35.65 PV.coast @01:36:35.70 PinPort.coast @01:36:35.88 Gate.power :motor,:OFF @01:36:35.89 Gate.power :main,:OFF @01:36:35.92 Gate.power :core,:OFF @01:36:35.94 Gate.power :analytic1,:OFF @01:36:35.96 Gate.power :analytic2,:OFF @01:36:35.98 Gate.power :analytic3,:OFF @01:36:36.00 Safely stopped and ready to power off ##-- file closed 2018-08-29T05:50:54.681Z ##-- file opened 2018-08-29T05:50:54.683Z @01:49:47.96 -> showStatus @01:49:49.97 -> Cmd.loadCartridge @01:49:50.10 Gate.power :main,:ON,limit to 5000mA @01:49:50.12 Gate.power :analytic1,:ON,limit to 5000mA @01:49:50.14 Gate.power :analytic2,:ON,limit to 5000mA @01:49:50.16 Gate.power :analytic3,:ON,limit to 5000mA @01:49:50.30 Powering core bus @01:49:52.61 SPR Module 1, FW Rev. B, FPGA Rev. C @01:49:52.71 ArS.configure ArSconfig @01:49:52.81 LyS1.configure LyS1config @01:49:52.93 LyS2.configure LyS2config @01:49:53.04 AirS.configure AirSconfig @01:49:53.16 TV.configure TVconfig @01:49:53.28 SP.configure SPconfig @01:49:53.39 SC.configure SCconfig @01:49:53.52 PV.configure PVconfig @01:49:53.64 PinPort.configure PPconfig @01:49:53.76 Sea.configure SPconfig @01:49:53.81 All elf controllers running firmware version 4.5 @01:49:53.83 Gate.power :motor,:ON,limit to 5000mA @01:49:54.18 ArS.reconfigure ArSconfig @01:49:54.24 LyS1.reconfigure LyS1config @01:49:54.31 LyS2.reconfigure LyS2config @01:49:54.37 AirS.reconfigure AirSconfig @01:49:54.49 TV.reconfigure TVconfig @01:49:54.58 PV.reconfigure PVconfig @01:49:54.61 ArS.home.jog 0 @01:49:54.66 LyS1.home.jog 0 @01:49:54.69 LyS2.home.jog 0 @01:49:54.74 PinPort.reconfigure PPconfig @01:49:54.76 AirS.home.jog 0 @01:49:54.88 TV.home.jog 0 @01:49:55.03 PV.home.jog 0 @01:49:55.14 PinPort.home.jog 0 @01:49:55.74 LyS2.reconfigure HomeSyringe @01:49:55.76 LyS1.reconfigure HomeSyringe @01:49:55.81 PinPort.reconfigure HomePP @01:49:55.83 TV.reconfigure HomeTV @01:49:55.87 AirS.reconfigure HomeMagSyringe @01:49:55.92 PV.reconfigure HomePV @01:49:55.98 ArS.reconfigure HomeMagSyringe @01:49:56.15 LyS2.jog -258 @01:49:56.17 PinPort.jog -111 @01:49:56.21 TV.jog -537 @01:49:56.25 LyS1.jog -266 @01:49:56.29 PV.jog -1480 @01:49:56.32 AirS.jog -319 @01:49:56.43 ArS.jog -228 @01:49:56.70 PinPort.setRawPosition! 0 @01:49:56.75 PinPort.seek :home @01:49:57.10 PinPort.reconfigure PPconfig @01:49:57.20 AirS.setRawPosition! -50 @01:49:57.21 LyS2.setRawPosition! -50 @01:49:57.23 LyS1.setRawPosition! -50 @01:49:57.26 ArS.setRawPosition! -50 @01:49:57.36 AirS.seek :home @01:49:57.42 LyS2.seek :home @01:49:57.45 LyS1.seek :home @01:49:57.58 ArS.seek :home @01:49:57.73 PV.setRawPosition! -50 @01:49:57.78 PV.seek :home @01:49:57.92 AirS.reconfigure AirSconfig @01:49:57.94 LyS2.reconfigure LyS2config @01:49:58.09 LyS1.reconfigure LyS1config @01:49:58.12 ArS.reconfigure ArSconfig @01:49:58.16 PV.reconfigure PVconfig @01:49:58.33 TV.setRawPosition! 0 @01:49:58.38 TV.seek :home @01:49:58.48 TV.reconfigure TVconfig @01:49:58.77 TV.seek :clear @01:50:00.38 TV.coast @01:50:00.44 SC.reconfigure HomeSC @01:50:00.50 SC.home.jog 0 @01:50:01.25 SC.reconfigure SCconfig @01:50:01.31 SC.seek 33 @01:50:01.41 Selecting Cartridge 32 @01:50:01.71 SC.seek 32 @01:50:02.22 LyS2.seek :locked @01:50:02.35 PV.seek :locked @01:50:02.48 AirS.seek :locked @01:50:06.56 Powering cartridge bus @01:50:08.69 Gate.setIR 1750,5000,0,6 @01:50:08.82 Gate.setIR 0,5000,0,6 @01:50:08.87 Loaded Lyse-n-Go Cartridge @01:50:08.88 PuckHeater.configure HeatConfig @01:50:08.94 Gate.power :heat,:ON,limit to 5000mA @01:50:28.73 -> Cmd.startFiltering @01:50:28.96 Duration of filtering limited to 1:05:00 @01:50:29.21 Priming sample loop w/100ml, bypass w/10ml @01:50:29.29 SP.setPosition! 0ml @01:50:29.32 Intake.open @01:50:29.35 Exhaust.open @01:50:30.21 Sea @-42.9psia @01:50:30.22 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @01:50:36.00 Vacuum tare is 25% of full scale @01:50:36.02 PB.open @01:50:36.06 Exhaust.close @01:50:36.85 Sea @-42.9psia @01:50:36.94 SP.reconfigure SPprime @01:50:37.01 SP.seek 10ml,1:21 @01:50:47.86 SP.setPosition! 0ml @01:50:47.90 PB.close @01:50:47.93 Exhaust.open @01:50:48.79 Sea @-42.9psia @01:50:48.87 SP.reconfigure SPprime @01:50:48.94 SP.seek 100ml,8:51 @01:52:40.38 Intake.close @01:52:41.13 Pressurizing cartridge to 26psi (try #1 of 3) @01:52:41.14 SP.setPosition! 0ml @01:52:41.18 PB.open @01:52:41.98 TV.seek :crack @01:52:42.77 TV.seek :clear @01:52:43.56 Intake.open @01:52:44.99 PB.close @01:52:45.72 Exhaust.close @01:52:46.47 Sea @-42.9psia @01:52:46.55 SP.reconfigure SPcharge @01:52:46.61 SP.seek 2.3ml,42 seconds @01:52:56.50 Cartridge pressurized with 0.456ml to 26.1psi @01:52:56.87 delay 3 seconds @01:52:59.94 Cartridge at 21.6psi 3 seconds later @01:53:00.02 TV.seek :cartridge @01:53:01.84 TV.seek :clear @01:53:03.66 Sampling 1000.0ml @01:53:03.67 SP.setPosition! 0ml @01:53:03.72 Exhaust.open @01:53:04.49 Sea @-42.9psia @01:53:04.58 SP.reconfigure SPsample @01:53:04.64 SP.seek 1000ml,1:23:53 @01:53:05.60 Cartridge 32 sampling 0/1000ml at 0.17ml/s with 3.1psi vacuum @01:54:05.61 Cartridge 32 sampling 33/1000ml at 0.57ml/s with 24.2psi vacuum @01:55:05.60 Cartridge 32 sampling 65/1000ml at 0.53ml/s with 20.6psi vacuum @01:56:05.61 Cartridge 32 sampling 98/1000ml at 0.55ml/s with 22.8psi vacuum @01:57:05.61 Cartridge 32 sampling 130/1000ml at 0.54ml/s with 24.4psi vacuum @01:58:05.61 Cartridge 32 sampling 162/1000ml at 0.51ml/s with 21.0psi vacuum @01:59:05.61 Cartridge 32 sampling 194/1000ml at 0.52ml/s with 20.4psi vacuum @02:00:05.60 Cartridge 32 sampling 225/1000ml at 0.51ml/s with 20.8psi vacuum @02:01:05.61 Cartridge 32 sampling 256/1000ml at 0.54ml/s with 24.0psi vacuum @02:02:05.61 Cartridge 32 sampling 287/1000ml at 0.48ml/s with 23.9psi vacuum @02:03:05.61 Cartridge 32 sampling 317/1000ml at 0.49ml/s with 21.8psi vacuum @02:04:05.61 Cartridge 32 sampling 346/1000ml at 0.47ml/s with 19.9psi vacuum @02:05:05.61 Cartridge 32 sampling 374/1000ml at 0.47ml/s with 24.1psi vacuum @02:06:05.61 Cartridge 32 sampling 401/1000ml at 0.46ml/s with 21.2psi vacuum @02:07:05.87 Cartridge 32 sampling 428/1000ml at 0.40ml/s with 24.5psi vacuum @02:08:05.61 Cartridge 32 sampling 453/1000ml at 0.41ml/s with 20.0psi vacuum @02:09:05.61 Cartridge 32 sampling 477/1000ml at 0.36ml/s with 20.7psi vacuum @02:10:05.61 Cartridge 32 sampling 500/1000ml at 0.34ml/s with 22.9psi vacuum @02:11:05.61 Cartridge 32 sampling 521/1000ml at 0.31ml/s with 25.1psi vacuum @02:12:05.61 Cartridge 32 sampling 541/1000ml at 0.32ml/s with 26.0psi vacuum @02:13:05.61 Cartridge 32 sampling 559/1000ml at 0.28ml/s with 23.9psi vacuum @02:14:05.61 Cartridge 32 sampling 576/1000ml at 0.24ml/s with 22.8psi vacuum @02:15:05.61 Cartridge 32 sampling 592/1000ml at 0.28ml/s with 24.0psi vacuum @02:16:05.61 Cartridge 32 sampling 607/1000ml at 0.22ml/s with 21.8psi vacuum @02:17:05.61 Cartridge 32 sampling 621/1000ml at 0.21ml/s with 23.3psi vacuum @02:18:05.61 Cartridge 32 sampling 634/1000ml at 0.20ml/s with 20.8psi vacuum @02:19:05.61 Cartridge 32 sampling 647/1000ml at 0.21ml/s with 21.4psi vacuum @02:20:05.61 Cartridge 32 sampling 659/1000ml at 0.22ml/s with 22.0psi vacuum @02:21:05.61 Cartridge 32 sampling 672/1000ml at 0.20ml/s with 25.9psi vacuum @02:22:05.61 Cartridge 32 sampling 684/1000ml at 0.20ml/s with 28.2psi vacuum @02:23:05.61 Cartridge 32 sampling 696/1000ml at 0.20ml/s with 29.3psi vacuum @02:23:31.31 Flow averaged 0.39ml/s over 30:22 @02:23:31.33 Waiting up to 16 seconds for vacuum to reduce from 29.1 to 18.0psi @02:23:31.95 Relieved excess vacuum in 6.2 seconds @02:23:31.98 SP.seek 1000ml,25:26 @02:24:01.24 Flow averaged 0.25ml/s over 24 seconds @02:24:01.27 Waiting up to 16 seconds for vacuum to reduce from 29.3 to 18.0psi @02:24:01.89 Relieved excess vacuum in 6.8 seconds @02:24:01.92 SP.seek 1000ml,24:57 @02:24:05.60 Cartridge 32 sampling 708/1000ml at 0.20ml/s with 31.4psi vacuum @02:24:18.26 Flow averaged 0.23ml/s over 14 seconds @02:24:18.28 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @02:24:18.90 Relieved excess vacuum in 3.2 seconds @02:24:18.93 SP.seek 1000ml,24:41 @02:24:32.07 Flow averaged 0.20ml/s over 14 seconds @02:24:32.09 Intake.close @02:24:32.14 Exhaust.close @02:24:32.92 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @02:24:33.01 Sampled 712.6ml @02:24:33.09 TV.seek :bypass @02:24:34.71 TV.seek :clear @02:24:36.81 -> Cmd.startProcessing @02:24:38.62 SPR.command \"read_sequences\" @02:24:39.02 SPR.command \"stats_on\" @02:24:39.06 SPR.command \"start 0 1\" @02:24:41.07 Begun log /var/log/esp/spr18aug2902h.spr @02:24:41.86 Handoff.select :waste @02:24:41.97 ArS.seek empty @02:24:59.68 PinPort.seek :cartridge @02:25:05.39 LyS1.seek 1350ul @02:25:11.33 PV.seek :reagent @02:25:12.41 AirS.seek :tVent @02:25:14.49 delay 2 seconds @02:25:16.52 AirS.reconfigure EvacAirS @02:25:16.58 AirS.seek :evac @02:25:38.86 delay 15 seconds @02:25:53.89 AirS.reconfigure AirSconfig @02:25:53.95 AirS.seek :tVent @02:26:08.15 delay 2 seconds @02:26:10.18 AirS.reconfigure EvacAirS @02:26:10.23 AirS.seek :evac @02:26:32.52 delay 15 seconds @02:26:47.55 AirS.reconfigure AirSconfig @02:26:47.61 AirS.seek :tVent @02:27:01.81 delay 2 seconds @02:27:03.84 AirS.reconfigure EvacAirS @02:27:03.90 AirS.seek :evac @02:27:26.18 delay 15 seconds @02:27:41.21 AirS.reconfigure AirSconfig @02:27:41.27 AirS.seek :tVent @02:27:55.47 delay 2 seconds @02:27:57.50 AirS.reconfigure EvacAirS @02:27:57.55 AirS.seek :evac @02:28:19.85 delay 15 seconds @02:28:34.88 AirS.reconfigure AirSconfig @02:28:34.94 AirS.seek :tVent @02:28:49.14 delay 2 seconds @02:28:51.17 AirS.reconfigure EvacAirS @02:28:51.22 AirS.seek :evac @02:29:13.51 delay 15 seconds @02:29:28.54 delay 1:30 @02:30:58.56 AirS.reconfigure AirSconfig @02:30:58.67 AirS.seek :fVent @02:31:00.10 delay 15 seconds @02:31:15.13 LyS1.reconfigure SlowLyS1 @02:31:15.24 LyS1.seek 1050ul @02:31:45.25 delay 5 seconds @02:31:50.28 Handoff.select :blocked @02:31:50.44 LyS1.seek 450ul @02:32:50.39 delay 10 seconds @02:33:00.42 AirS.reconfigure SlowAirS @02:33:00.53 AirS.reconfigure to mix into 1.37806 parts LyS1 @02:33:00.89 AirS.seek evac @02:33:00.93 LyS1.seek 300ul @02:33:16.03 AirS.reconfigure AirSconfig @02:33:16.10 AirS.seek :full @02:33:30.30 LyS1.seek :empty @02:34:00.39 PuckHeater.seek 84.1C @02:35:02.80 PuckHeater.hold 15:00,84.1C @02:50:01.82 LyS1.reconfigure LyS1config @02:50:01.88 LyS1.seek 900ul @02:50:10.21 AirS.seek :tVent @02:50:10.29 delay 2 seconds @02:50:12.31 AirS.reconfigure HalfSpeedAirS @02:50:12.37 AirS.seek :evac @02:50:40.16 delay 15 seconds @02:50:55.19 AirS.reconfigure AirSconfig @02:50:55.26 AirS.seek :tVent @02:51:09.47 delay 2 seconds @02:51:11.49 AirS.reconfigure HalfSpeedAirS @02:51:11.55 AirS.seek :evac @02:51:39.27 delay 15 seconds @02:51:54.30 AirS.reconfigure AirSconfig @02:51:54.36 AirS.seek :tVent @02:52:08.58 delay 2 seconds @02:52:10.60 AirS.reconfigure HalfSpeedAirS @02:52:10.66 AirS.seek :evac @02:52:38.39 delay 15 seconds @02:52:53.42 delay 1:00 @02:53:53.44 Handoff.select :waste @02:53:53.54 delay 15 seconds @02:54:08.56 AirS.seek :fVent @02:54:10.75 delay 10 seconds @02:54:20.78 AirS.reconfigure AirSconfig @02:54:20.83 AirS.seek :evac @02:54:22.27 Lysis Recovered in LyS1 @02:54:22.28 Lysis Complete @02:54:22.30 Handoff.select :waste @02:54:22.33 LyS2.seek :full @02:54:22.43 LyS1.reconfigure DAmixLyS1 @02:54:22.49 LyS2.reconfigure DAmixLyS2 @02:54:22.88 LyS2.seek 1480ul @02:54:22.92 LyS1.seek 650ul @02:55:10.24 delay 5 seconds @02:55:15.27 Handoff.select :SPR @02:55:15.38 SPR.command \"start 2 14\" @02:55:16.77 Running Experiment 2 @02:55:16.78 delay 10 seconds @02:55:26.80 SPR.command \"start 2 0\" @02:55:30.63 Waiting up to 11:00 for SPR to enter state 1 @03:02:49.38 SPR.command \"hold_on\" @03:02:51.07 LyS2.seek 1130ul @03:02:51.11 LyS1.seek 300ul @03:03:57.27 delay 15 seconds @03:04:12.29 SPR.command \"hold_off\" @03:04:15.08 Waiting up to 25:00 for SPR to enter state 7 @03:15:51.57 Handoff.select :waste @03:15:51.69 LyS1.reconfigure LyS1config @03:15:51.75 LyS2.reconfigure LyS2config @03:15:51.81 Rep #1 SPR Analysis has Completed @03:15:52.49 Ended log /var/log/esp/spr18aug2902h.spr @03:15:52.52 SPR.command \"stats_off\" @03:15:54.55 -> Cmd.stop @03:15:54.64 Handoff.select :close @03:15:54.82 Gate.power :heat,:OFF @03:15:54.83 Gate.power :cartridge,:OFF @03:15:54.87 PV.seek :locked @03:15:55.75 PinPort.seek :clear @03:16:01.76 ArS.seek :clear @03:16:01.89 LyS1.seek :clear @03:16:02.03 LyS2.seek :clear @03:16:02.16 AirS.seek :clear @03:16:19.52 ArS.coast @03:16:19.57 LyS1.coast @03:16:19.62 LyS2.coast @03:16:19.67 AirS.coast @03:16:19.72 TV.coast @03:16:19.77 SP.coast @03:16:19.82 SC.coast @03:16:19.87 PV.coast @03:16:19.92 PinPort.coast @03:16:20.11 Gate.power :motor,:OFF @03:16:20.12 Gate.power :main,:OFF @03:16:20.15 Gate.power :core,:OFF @03:16:20.17 Gate.power :analytic1,:OFF @03:16:20.19 Gate.power :analytic2,:OFF @03:16:20.21 Gate.power :analytic3,:OFF @03:16:20.22 Safely stopped and ready to power off ##-- file closed 2018-08-29T07:28:27.956Z ##-- file opened 2018-08-29T07:28:27.958Z @03:27:12.28 -> showStatus @03:27:14.27 -> Cmd.loadCartridge @03:27:14.39 Gate.power :main,:ON,limit to 5000mA @03:27:14.41 Gate.power :analytic1,:ON,limit to 5000mA @03:27:14.43 Gate.power :analytic2,:ON,limit to 5000mA @03:27:14.45 Gate.power :analytic3,:ON,limit to 5000mA @03:27:14.59 Powering core bus @03:27:16.89 SPR Module 1, FW Rev. B, FPGA Rev. C @03:27:17.00 ArS.configure ArSconfig @03:27:17.10 LyS1.configure LyS1config @03:27:17.21 LyS2.configure LyS2config @03:27:17.32 AirS.configure AirSconfig @03:27:17.44 TV.configure TVconfig @03:27:17.56 SP.configure SPconfig @03:27:17.68 SC.configure SCconfig @03:27:17.80 PV.configure PVconfig @03:27:17.92 PinPort.configure PPconfig @03:27:18.04 Sea.configure SPconfig @03:27:18.10 All elf controllers running firmware version 4.5 @03:27:18.11 Gate.power :motor,:ON,limit to 5000mA @03:27:18.31 ArS.reconfigure ArSconfig @03:27:18.51 ArS.home.jog 0 @03:27:18.66 PV.reconfigure PVconfig @03:27:18.74 TV.reconfigure TVconfig @03:27:18.77 LyS2.reconfigure LyS2config @03:27:18.81 PinPort.reconfigure PPconfig @03:27:18.84 LyS1.reconfigure LyS1config @03:27:18.90 AirS.reconfigure AirSconfig @03:27:19.09 PV.home.jog 0 @03:27:19.13 TV.home.jog 0 @03:27:19.17 LyS2.home.jog 0 @03:27:19.20 PinPort.home.jog 0 @03:27:19.22 AirS.home.jog 0 @03:27:19.28 LyS1.home.jog 0 @03:27:19.61 ArS.reconfigure HomeMagSyringe @03:27:19.97 TV.reconfigure HomeTV @03:27:20.00 AirS.reconfigure HomeMagSyringe @03:27:20.04 LyS2.reconfigure HomeSyringe @03:27:20.07 ArS.jog -224 @03:27:20.12 PV.reconfigure HomePV @03:27:20.15 PinPort.reconfigure HomePP @03:27:20.20 LyS1.reconfigure HomeSyringe @03:27:20.36 TV.jog -537 @03:27:20.42 LyS2.jog -258 @03:27:20.45 AirS.jog -329 @03:27:20.49 PinPort.jog -112 @03:27:20.53 PV.jog -1480 @03:27:20.61 LyS1.jog -269 @03:27:20.79 ArS.setRawPosition! -50 @03:27:20.84 ArS.seek :home @03:27:21.01 PinPort.setRawPosition! 0 @03:27:21.06 PinPort.seek :home @03:27:21.43 PinPort.reconfigure PPconfig @03:27:21.47 AirS.setRawPosition! -50 @03:27:21.49 LyS2.setRawPosition! -50 @03:27:21.52 LyS1.setRawPosition! -50 @03:27:21.54 ArS.reconfigure ArSconfig @03:27:21.68 AirS.seek :home @03:27:21.74 LyS2.seek :home @03:27:21.79 LyS1.seek :home @03:27:21.99 PV.setRawPosition! -50 @03:27:22.04 PV.seek :home @03:27:22.18 AirS.reconfigure AirSconfig @03:27:22.26 LyS2.reconfigure LyS2config @03:27:22.31 LyS1.reconfigure LyS1config @03:27:22.45 TV.setRawPosition! 0 @03:27:22.47 PV.reconfigure PVconfig @03:27:22.56 TV.seek :home @03:27:22.66 TV.reconfigure TVconfig @03:27:22.89 TV.seek :clear @03:27:24.51 TV.coast @03:27:24.57 SC.reconfigure HomeSC @03:27:24.63 SC.home.jog 0 @03:27:25.40 SC.reconfigure SCconfig @03:27:25.46 SC.seek 32 @03:27:25.52 Selecting Cartridge 31 @03:27:25.83 SC.seek 31 @03:27:26.32 LyS2.seek :locked @03:27:26.45 PV.seek :locked @03:27:26.59 AirS.seek :locked @03:27:30.67 Powering cartridge bus @03:27:32.79 Gate.setIR 1750,5000,0,6 @03:27:32.92 Gate.setIR 0,5000,0,6 @03:27:32.95 Loaded Archive Cartridge @03:27:32.97 Gate.power :cartridge,:OFF @03:27:52.62 -> Cmd.startFiltering @03:27:52.84 Duration of filtering limited to 1:05:00 @03:27:53.10 Priming sample loop w/100ml, bypass w/10ml @03:27:53.17 SP.setPosition! 0ml @03:27:53.22 Intake.open @03:27:53.24 Exhaust.open @03:27:54.08 Sea @-42.9psia @03:27:54.10 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @03:27:59.88 Vacuum tare is 25% of full scale @03:27:59.90 PB.open @03:27:59.94 Exhaust.close @03:28:00.72 Sea @-42.9psia @03:28:00.80 SP.reconfigure SPprime @03:28:00.86 SP.seek 10ml,1:21 @03:28:11.72 SP.setPosition! 0ml @03:28:11.76 PB.close @03:28:11.79 Exhaust.open @03:28:12.59 Sea @-42.9psia @03:28:12.67 SP.reconfigure SPprime @03:28:12.74 SP.seek 100ml,8:51 @03:30:06.50 Intake.close @03:30:07.25 Pressurizing cartridge to 26psi (try #1 of 3) @03:30:07.26 SP.setPosition! 0ml @03:30:07.31 PB.open @03:30:08.09 TV.seek :crack @03:30:08.88 TV.seek :clear @03:30:09.67 Intake.open @03:30:11.14 PB.close @03:30:11.84 Exhaust.close @03:30:12.59 Sea @-42.9psia @03:30:12.67 SP.reconfigure SPcharge @03:30:12.73 SP.seek 2.3ml,42 seconds @03:30:20.46 Cartridge pressurized with 0.277ml to 26.1psi @03:30:20.68 delay 3 seconds @03:30:23.75 Cartridge at 20.5psi 3 seconds later @03:30:23.83 TV.seek :cartridge @03:30:25.65 TV.seek :clear @03:30:27.47 Sampling 1000.0ml @03:30:27.48 SP.setPosition! 0ml @03:30:27.53 Exhaust.open @03:30:28.28 Sea @-42.9psia @03:30:28.37 SP.reconfigure SPsample @03:30:28.43 SP.seek 1000ml,1:23:53 @03:30:29.39 Cartridge 31 sampling 0/1000ml at 0.17ml/s with 7.5psi vacuum @03:31:29.40 Cartridge 31 sampling 24/1000ml at 0.41ml/s with 20.6psi vacuum @03:32:29.40 Cartridge 31 sampling 47/1000ml at 0.33ml/s with 24.5psi vacuum @03:33:29.40 Cartridge 31 sampling 67/1000ml at 0.28ml/s with 23.3psi vacuum @03:34:29.40 Cartridge 31 sampling 86/1000ml at 0.28ml/s with 24.4psi vacuum @03:35:29.40 Cartridge 31 sampling 102/1000ml at 0.28ml/s with 19.0psi vacuum @03:36:29.40 Cartridge 31 sampling 118/1000ml at 0.21ml/s with 21.2psi vacuum @03:37:29.39 Cartridge 31 sampling 131/1000ml at 0.24ml/s with 20.2psi vacuum @03:38:29.40 Cartridge 31 sampling 144/1000ml at 0.23ml/s with 21.8psi vacuum @03:39:29.40 Cartridge 31 sampling 156/1000ml at 0.20ml/s with 25.2psi vacuum @03:39:55.28 Flow averaged 0.29ml/s over 9:22 @03:39:55.30 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @03:39:55.91 Relieved excess vacuum in 6.4 seconds @03:39:55.94 SP.seek 1000ml,1:10:24 @03:40:15.61 Flow averaged 0.29ml/s over 14 seconds @03:40:15.63 Waiting up to 14 seconds for vacuum to reduce from 28.6 to 18.0psi @03:40:16.25 Relieved excess vacuum in 7.2 seconds @03:40:16.28 SP.seek 1000ml,1:10:05 @03:40:26.39 Flow estimate reduced to 0.23ml/s @03:40:26.43 Waiting up to 14 seconds for vacuum to reduce from 28.2 to 18.0psi @03:40:27.31 Relieved excess vacuum in 7.7 seconds @03:40:27.34 SP.seek 1000ml,1:09:56 @03:40:29.39 Cartridge 31 sampling 168/1000ml at 0.20ml/s with 30.5psi vacuum @03:40:32.65 Flow estimate reduced to 0.19ml/s @03:40:32.67 Intake.close @03:40:32.71 Exhaust.close @03:40:33.55 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @03:40:33.65 Sampled 168.3ml @03:40:33.73 TV.seek :bypass @03:40:35.34 TV.seek :clear @03:40:37.46 -> Cmd.startProcessing @03:40:37.72 PV.seek :reagent @03:40:38.56 Sample loop closed. Ready to Deliver Reagents @03:40:38.58 ArS.seek :sealed @03:40:42.85 ArS.seek :evac @03:40:56.78 delay 20 seconds @03:41:16.81 ArS.seek :clear @03:41:34.52 RNAlater soak for 10:00 @03:51:34.55 ArS.seek :tVent @03:51:37.13 delay 1 second @03:51:38.15 ArS.seek :evac @03:51:53.77 delay 10 seconds @03:52:03.80 Sample preserved and evacuated @03:52:04.82 -> Cmd.stop @03:52:04.96 PV.seek :locked @03:52:05.85 PinPort.seek :clear @03:52:05.99 ArS.seek :clear @03:52:06.17 LyS2.seek :clear @03:52:06.31 AirS.seek :clear @03:52:23.73 ArS.coast @03:52:23.78 LyS1.coast @03:52:23.83 LyS2.coast @03:52:23.88 AirS.coast @03:52:23.93 TV.coast @03:52:23.98 SP.coast @03:52:24.03 SC.coast @03:52:24.09 PV.coast @03:52:24.14 PinPort.coast @03:52:24.32 Gate.power :motor,:OFF @03:52:24.33 Gate.power :main,:OFF @03:52:24.36 Gate.power :core,:OFF @03:52:24.38 Gate.power :analytic1,:OFF @03:52:24.40 Gate.power :analytic2,:OFF @03:52:24.42 Gate.power :analytic3,:OFF @03:52:24.44 Safely stopped and ready to power off ##-- file closed 2018-08-29T08:43:34.927Z