##-- file opened 2022-05-13T22:06:02.662Z @15:06:02.89 -> showStatus @15:06:04.50 -> Cmd.loadCartridge -6 @15:06:04.56 Gate.power :main,:ON,limit to 5000mA @15:06:04.58 Gate.power :analytic1,:ON,limit to 5000mA @15:06:04.60 Gate.power :analytic2,:ON,limit to 5000mA @15:06:04.63 Gate.power :analytic3,:ON,limit to 5000mA @15:06:04.68 Powering core bus @15:06:07.41 Archive.configure :default @15:06:07.53 Air.configure :default @15:06:07.63 Lysis1.configure :default @15:06:07.74 Lysis2.configure :default @15:06:07.87 Plunge.configure :default @15:06:08.00 SamplePump.configure :default @15:06:08.13 Toroid.configure :default @15:06:08.27 Carousel.configure :default @15:06:08.40 Pressure.configure :default @15:06:08.52 ExtraPump.configure :default @15:06:08.58 All elf controllers running firmware version 4.794 @15:06:08.61 Gate.power :motor,:ON,limit to 5000mA @15:06:08.85 Archive.in :default @15:06:09.07 Archive.home.jog 0 @15:06:09.20 Plunge.in :default @15:06:09.26 Lysis2.in :default @15:06:09.29 Air.in :default @15:06:09.33 Toroid.in :default @15:06:09.39 Lysis1.in :default @15:06:09.57 Plunge.home.jog 0 @15:06:09.61 Lysis2.home.jog 0 @15:06:09.64 Toroid.home.jog 0 @15:06:09.66 Air.home.jog 0 @15:06:09.71 Lysis1.home.jog 0 @15:06:10.02 Archive.in :homing @15:06:10.18 Plunge.in :homing @15:06:10.21 Lysis2.in :homing @15:06:10.24 Archive.jog 1368 @15:06:10.30 Toroid.in :homing @15:06:10.48 Lysis2.jog 1351 @15:06:10.53 Plunge.jog 301 @15:06:10.57 Toroid.jog 612 @15:06:10.75 Lysis1.in :homing @15:06:10.76 Air.in :homing @15:06:10.86 Lysis1.jog 1364 @15:06:10.88 Air.jog 1359 @15:06:11.18 Plunge.setRawPosition! 50 @15:06:11.23 Plunge.seek :home @15:06:11.50 Plunge.in :default @15:06:11.58 Archive.setRawPosition! 50 @15:06:11.63 Archive.seek :home @15:06:11.73 Lysis2.setRawPosition! 50 @15:06:11.78 Lysis2.seek :home @15:06:11.90 Archive.in :default @15:06:12.06 Lysis1.setRawPosition! 50 @15:06:12.08 Lysis2.in :default @15:06:12.15 Lysis1.seek :home @15:06:12.27 Air.setRawPosition! 50 @15:06:12.32 Air.seek :home @15:06:12.46 Lysis1.in :default @15:06:12.59 Air.in :default @15:06:12.70 Toroid.setRawPosition! 0 @15:06:12.75 Toroid.seek :home @15:06:12.84 Toroid.in :default @15:06:12.98 Toroid.seek :clear @15:06:14.59 Carousel.in :default @15:06:14.65 Carousel.home.jog 0 @15:06:15.13 Carousel.in :default @15:06:15.20 Carousel.seek 10 @15:06:15.34 Selecting Cartridge 60 @15:06:15.54 Carousel.seek 60 @15:06:26.00 Lysis1.in :fast @15:06:26.06 Lysis1.seek :locked @15:06:28.30 Plunge.seek :locked @15:06:28.42 Air.in :fast @15:06:28.48 Air.seek :locked @15:06:31.40 Mounted archive Cartridge @15:06:38.85 -> Cmd.startFiltering 17.07@15:06:39.09 Preparing to filter cartridge 60 @15:06:39.37 Priming sample loop w/100ml, bypass w/10ml @15:06:39.38 SamplePump.setPosition! 0ml @15:06:39.44 Bypass.open @15:06:40.84 Intake.open @15:06:41.51 Sea 2.1m deep (17.7psia) @15:06:41.54 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:06:44.11 Vacuum tare is 50% of full scale @15:06:44.23 Pressure.in :default @15:06:44.29 Pressure.hold @15:06:44.47 SamplePump.in :prime @15:06:44.91 SamplePump.seek 10ml,59 seconds @15:06:54.87 Pressure.stop @15:06:54.92 SamplePump.setPosition! 0ml @15:06:54.98 Exhaust.open @15:06:56.35 Bypass.close @15:06:57.85 Pressure.in :default @15:06:57.91 Pressure.hold @15:06:58.03 Sea 2.1m deep (17.7psia) @15:06:58.17 SamplePump.in :prime @15:06:58.24 SamplePump.seek 100ml,5:16 @15:08:34.65 Pressure.stop @15:08:34.71 Bypass.open @15:08:35.38 Intake.close @15:08:36.08 Toroid.in :hold @15:08:36.14 Toroid.seek :crack @15:08:37.77 Toroid.seek :cartridge @15:08:38.73 Toroid.in :default @15:08:38.79 Toroid.seek :clear @15:08:40.75 Pressurizing cartridge to 26psi above 17.9psia @15:08:40.77 SamplePump.setPosition! 0ml @15:08:40.82 Intake.open @15:08:41.46 Bypass.close @15:08:42.13 Exhaust.close @15:08:42.95 Pressure.in :default @15:08:43.01 Pressure.hold @15:08:43.18 SamplePump.in :charge @15:08:43.25 SamplePump.seek 2.3ml,42 seconds @15:08:52.51 Pressure.stop @15:08:52.56 Intake.close @15:08:53.23 Pressurized cartridge with 0.394ml to 26.5psi @15:08:53.24 Isolated @42.6psia (24.7psi) @15:08:53.25 Verifying cartridge holds at least 0.65..0.85 of 42.6psia @15:08:53.29 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:08:57.87 Cartridge pressure fell 1% to 42.1psia @15:08:57.95 Sampling 100.0ml @15:08:57.96 SamplePump.setPosition! 0ml @15:08:58.02 Exhaust.open @15:08:58.69 Intake.open @15:08:59.44 Pressure.in :default @15:08:59.49 Pressure.hold @15:08:59.61 Sea 3.5m deep (19.8psia) @15:08:59.75 SamplePump.in :sample @15:08:59.82 SamplePump.seek 100ml,8:50 @15:09:02.47 Cartridge 60 filtering 1/100ml at 0.48ml/s with 23.3psi vacuum 2.0m deep (17.7psia) @15:10:02.46 Cartridge 60 filtering 28/100ml at 0.43ml/s with 20.7psi vacuum 2.6m deep (18.5psia) @15:11:02.47 Cartridge 60 filtering 52/100ml at 0.33ml/s with 23.9psi vacuum @15:12:02.46 Cartridge 60 filtering 71/100ml at 0.28ml/s with 21.2psi vacuum 1.8m deep (17.4psia) @15:13:02.47 Cartridge 60 filtering 86/100ml at 0.20ml/s with 24.3psi vacuum @15:13:55.06 Flow averaged 0.33ml/s over 4:56 @15:13:55.10 Waiting up to 14 seconds for vacuum to reduce from 28.6 to 18.0psi @15:13:55.93 Relieved excess vacuum in .9 seconds @15:13:55.95 SamplePump.seek 100ml,44 seconds @15:14:02.46 Cartridge 60 filtering 99/100ml at 0.20ml/s with 25.2psi vacuum @15:14:05.04 Flow averaged 0.20ml/s over 10 seconds @15:14:05.09 Waiting up to 12 seconds for vacuum to reduce from 27.7 to 18.0psi @15:14:05.91 Relieved excess vacuum in .9 seconds @15:14:05.93 SamplePump.seek 100ml,35 seconds @15:14:09.19 Flow estimate reduced to 0.16ml/s @15:14:09.21 Intake.close @15:14:09.27 Exhaust.close @15:14:09.45 Toroid.in :hold @15:14:09.52 Toroid.seek :bypass @15:14:11.14 Toroid.in :default @15:14:11.21 Toroid.seek :clear @15:14:12.81 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @15:14:12.83 Pressure.stop @15:14:13.02 Sampled 99.7ml @15:14:14.96 -> Cmd.startProcessing @15:14:15.35 Plunge.seek :reagent @15:14:16.26 Sample loop closed. Ready to Deliver Reagents @15:14:16.28 Archive.seek :sealed @15:14:19.93 Archive.seek :evac @15:14:33.92 delay 1:00 @15:15:33.95 Archive.seek :clear @15:15:51.11 RNAlater soak for 10:00 @15:16:15.12 Session Begun @15:16:15.15 -> spare BACsampler: 1000 {BACsampler: 1000} @15:16:15.22 Session Ended @15:25:51.14 Archive.seek :tVent @15:25:52.78 delay 1 second @15:25:53.80 Archive.seek :evac @15:26:09.78 delay 10 seconds @15:26:19.81 Sample preserved and evacuated @15:26:20.96 -> Cmd.stop @15:26:21.10 Carousel.seek 60 @15:26:21.29 Plunge.seek :locked @15:26:22.24 Archive.in :unlocking @15:26:22.36 Archive.seek :clear @15:26:22.44 Air.in :unlocking @15:26:22.56 Lysis1.in :unlocking @15:26:22.59 Air.seek :clear @15:26:22.75 Lysis1.seek :clear @15:26:26.42 Gate.power :motor,:OFF @15:26:26.43 Gate.power :core,:OFF @15:26:26.45 Gate.power :analytic1,:OFF @15:26:26.47 Gate.power :analytic2,:OFF @15:26:26.50 Gate.power :analytic3,:OFF @15:26:26.52 Gate.power :main,:OFF @15:26:26.55 Safely stopped and ready to power off ##-- file closed 2022-05-13T23:20:45.270Z ##-- file opened 2022-05-13T23:20:45.296Z @16:20:45.10 -> showStatus @16:20:47.12 -> Cmd.loadCartridge -6 @16:20:47.19 Gate.power :main,:ON,limit to 5000mA @16:20:47.21 Gate.power :analytic1,:ON,limit to 5000mA @16:20:47.23 Gate.power :analytic2,:ON,limit to 5000mA @16:20:47.26 Gate.power :analytic3,:ON,limit to 5000mA @16:20:47.32 Powering core bus @16:20:50.04 Archive.configure :default @16:20:50.18 Air.configure :default @16:20:50.29 Lysis1.configure :default @16:20:50.40 Lysis2.configure :default @16:20:50.53 Plunge.configure :default @16:20:50.66 SamplePump.configure :default @16:20:50.79 Toroid.configure :default @16:20:50.92 Carousel.configure :default @16:20:51.05 Pressure.configure :default @16:20:51.18 ExtraPump.configure :default @16:20:51.24 All elf controllers running firmware version 4.794 @16:20:51.27 Gate.power :motor,:ON,limit to 5000mA @16:20:51.56 Archive.in :default @16:20:51.81 Toroid.in :default @16:20:51.85 Plunge.in :default @16:20:51.88 Lysis1.in :default @16:20:51.93 Archive.home.jog 0 @16:20:51.96 Lysis2.in :default @16:20:52.01 Air.in :default @16:20:52.18 Toroid.home.jog 0 @16:20:52.26 Plunge.home.jog 0 @16:20:52.29 Lysis1.home.jog 0 @16:20:52.31 Air.home.jog 0 @16:20:52.34 Lysis2.home.jog 0 @16:20:52.77 Plunge.in :homing @16:20:52.86 Toroid.in :homing @16:20:52.93 Archive.in :homing @16:20:53.04 Plunge.jog 301 @16:20:53.09 Toroid.jog 602 @16:20:53.11 Archive.jog 1364 @16:20:53.32 Lysis2.in :homing @16:20:53.34 Lysis1.in :homing @16:20:53.37 Air.in :homing @16:20:53.50 Lysis2.jog 1349 @16:20:53.54 Air.jog 1355 @16:20:53.58 Lysis1.jog 1364 @16:20:53.74 Plunge.setRawPosition! 50 @16:20:53.78 Plunge.seek :home @16:20:54.05 Plunge.in :default @16:20:54.33 Archive.setRawPosition! 50 @16:20:54.38 Archive.seek :home @16:20:54.65 Archive.in :default @16:20:54.74 Lysis2.setRawPosition! 50 @16:20:54.81 Lysis2.seek :home @16:20:54.89 Lysis1.setRawPosition! 50 @16:20:54.91 Air.setRawPosition! 50 @16:20:55.00 Lysis1.seek :home @16:20:55.02 Air.seek :home @16:20:55.16 Lysis2.in :default @16:20:55.25 Toroid.setRawPosition! 0 @16:20:55.30 Toroid.seek :home @16:20:55.42 Toroid.in :default @16:20:55.44 Lysis1.in :default @16:20:55.47 Air.in :default @16:20:55.94 Toroid.seek :clear @16:20:57.56 Carousel.in :default @16:20:57.62 Carousel.home.jog 0 @16:20:58.11 Carousel.in :default @16:20:58.18 Carousel.seek 60 @16:20:58.32 Selecting Cartridge 59 @16:20:58.53 Carousel.seek 59 @16:20:59.00 Lysis1.in :fast @16:20:59.07 Lysis1.seek :locked @16:21:01.31 Plunge.seek :locked @16:21:01.45 Air.in :fast @16:21:01.51 Air.seek :locked @16:21:04.43 Mounted archive Cartridge @16:21:21.47 -> Cmd.startFiltering @16:21:21.70 Preparing to filter cartridge 5992.07 @16:21:21.98 Priming sample loop w/100ml, bypass w/10ml @16:21:21.99 SamplePump.setPosition! 0ml @16:21:22.05 Bypass.open @16:21:23.49 Intake.open @16:21:24.15 Sea 2.0m deep (17.6psia) @16:21:24.17 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:21:26.74 Vacuum tare is 50% of full scale @16:21:26.88 Pressure.in :default @16:21:26.95 Pressure.hold @16:21:27.50 SamplePump.in :prime @16:21:27.58 SamplePump.seek 10ml,59 seconds @16:21:37.54 Pressure.stop @16:21:37.59 SamplePump.setPosition! 0ml @16:21:37.64 Exhaust.open @16:21:39.00 Bypass.close @16:21:40.52 Pressure.in :default @16:21:40.59 Pressure.hold @16:21:40.71 Sea 2.1m deep (17.8psia) @16:21:40.85 SamplePump.in :prime @16:21:40.92 SamplePump.seek 100ml,5:16 @16:23:17.32 Pressure.stop @16:23:17.38 Bypass.open @16:23:18.07 Intake.close @16:23:18.74 Toroid.in :hold @16:23:18.80 Toroid.seek :crack @16:23:20.43 Toroid.seek :cartridge @16:23:21.39 Toroid.in :default @16:23:21.46 Toroid.seek :clear @16:23:23.42 Pressurizing cartridge to 26psi above 18.0psia @16:23:23.44 SamplePump.setPosition! 0ml @16:23:23.49 Intake.open @16:23:24.09 Bypass.close @16:23:24.75 Exhaust.close @16:23:25.53 Pressure.in :default @16:23:25.60 Pressure.hold @16:23:25.77 SamplePump.in :charge @16:23:25.84 SamplePump.seek 2.3ml,42 seconds @16:23:34.86 Pressure.stop @16:23:34.91 Intake.close @16:23:35.57 Pressurized cartridge with 0.374ml to 26.1psi @16:23:35.58 Isolated @42.2psia (24.2psi) @16:23:35.60 Verifying cartridge holds at least 0.65..0.85 of 42.2psia @16:23:35.63 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:23:40.27 Sampling 1000.0ml @16:23:40.29 SamplePump.setPosition! 0ml @16:23:40.34 Exhaust.open @16:23:40.96 Intake.open @16:23:41.75 Pressure.in :default @16:23:41.81 Pressure.hold @16:23:41.92 Sea 3.2m deep (19.4psia) @16:23:42.06 SamplePump.in :sample @16:23:42.13 SamplePump.seek 1000ml,1:23:50 @16:23:44.77 Cartridge 59 filtering 1/1000ml at 0.47ml/s with 24.9psi vacuum 2.0m deep (17.6psia) @16:24:44.77 Cartridge 59 filtering 30/1000ml at 0.51ml/s with 24.1psi vacuum 2.3m deep (18.1psia) @16:25:44.77 Cartridge 59 filtering 60/1000ml at 0.51ml/s with 24.5psi vacuum 2.3m deep (18.1psia) @16:26:44.77 Cartridge 59 filtering 90/1000ml at 0.51ml/s with 25.4psi vacuum @16:27:44.77 Cartridge 59 filtering 120/1000ml at 0.52ml/s with 23.5psi vacuum 3.0m deep (19.1psia) @16:28:44.77 Cartridge 59 filtering 150/1000ml at 0.50ml/s with 21.2psi vacuum 2.9m deep (19.0psia) @16:29:44.77 Cartridge 59 filtering 180/1000ml at 0.49ml/s with 27.5psi vacuum @16:30:44.77 Cartridge 59 filtering 210/1000ml at 0.48ml/s with 27.0psi vacuum @16:31:44.77 Cartridge 59 filtering 240/1000ml at 0.50ml/s with 21.0psi vacuum 3.1m deep (19.2psia) @16:32:44.77 Cartridge 59 filtering 270/1000ml at 0.51ml/s with 25.4psi vacuum @16:33:44.77 Cartridge 59 filtering 300/1000ml at 0.52ml/s with 21.2psi vacuum 4.5m deep (21.3psia) @16:34:44.77 Cartridge 59 filtering 330/1000ml at 0.51ml/s with 23.8psi vacuum @16:35:44.77 Cartridge 59 filtering 361/1000ml at 0.51ml/s with 21.5psi vacuum 3.6m deep (20.0psia) @16:36:44.77 Cartridge 59 filtering 391/1000ml at 0.51ml/s with 21.6psi vacuum 3.5m deep (19.8psia) @16:37:44.77 Cartridge 59 filtering 421/1000ml at 0.51ml/s with 22.0psi vacuum 3.4m deep (19.7psia) @16:38:44.77 Cartridge 59 filtering 451/1000ml at 0.51ml/s with 21.5psi vacuum @16:39:44.77 Cartridge 59 filtering 482/1000ml at 0.53ml/s with 23.4psi vacuum 3.2m deep (19.4psia) @16:40:44.77 Cartridge 59 filtering 512/1000ml at 0.51ml/s with 26.5psi vacuum @16:41:44.77 Cartridge 59 filtering 542/1000ml at 0.50ml/s with 27.7psi vacuum 1.9m deep (17.5psia) @16:42:44.77 Cartridge 59 filtering 573/1000ml at 0.50ml/s with 20.4psi vacuum 3.8m deep (20.4psia) @16:43:44.77 Cartridge 59 filtering 603/1000ml at 0.52ml/s with 26.3psi vacuum @16:44:44.77 Cartridge 59 filtering 634/1000ml at 0.49ml/s with 19.3psi vacuum 2.7m deep (18.7psia) @16:45:44.77 Cartridge 59 filtering 664/1000ml at 0.52ml/s with 26.6psi vacuum @16:46:44.77 Cartridge 59 filtering 694/1000ml at 0.52ml/s with 23.7psi vacuum @16:47:44.77 Cartridge 59 filtering 725/1000ml at 0.49ml/s with 19.6psi vacuum 2.5m deep (18.4psia) @16:48:44.77 Cartridge 59 filtering 755/1000ml at 0.48ml/s with 27.1psi vacuum @16:49:44.77 Cartridge 59 filtering 786/1000ml at 0.50ml/s with 28.3psi vacuum @16:50:44.77 Cartridge 59 filtering 816/1000ml at 0.52ml/s with 23.9psi vacuum 3.2m deep (19.5psia) @16:51:44.77 Cartridge 59 filtering 847/1000ml at 0.53ml/s with 26.1psi vacuum 2.2m deep (18.0psia) @16:52:44.77 Cartridge 59 filtering 878/1000ml at 0.52ml/s with 26.3psi vacuum @16:53:44.77 Cartridge 59 filtering 908/1000ml at 0.53ml/s with 24.1psi vacuum 5.3m deep (22.6psia) @16:54:44.78 Cartridge 59 filtering 939/1000ml at 0.51ml/s with 28.6psi vacuum 1.8m deep (17.3psia) @16:55:44.77 Cartridge 59 filtering 969/1000ml at 0.49ml/s with 27.7psi vacuum @16:56:44.80 Pressure.stop @16:56:44.82 Cartridge 59 filtering 1000/1000ml at 0.50ml/s with 19.5psi vacuum 2.6m deep (18.5psia) @16:56:44.95 Intake.close @16:56:45.00 Exhaust.close @16:56:45.15 Toroid.in :hold @16:56:45.22 Toroid.seek :bypass @16:56:46.83 Toroid.in :default @16:56:46.89 Toroid.seek :clear @16:56:48.50 Sampled 1000.0ml @16:56:49.80 -> Cmd.startProcessing @16:56:50.22 Plunge.seek :reagent @16:56:51.11 Sample loop closed. Ready to Deliver Reagents @16:56:51.13 Archive.seek :sealed @16:56:54.76 Archive.seek :evac @16:57:08.74 delay 1:00 @16:58:08.77 Archive.seek :clear @16:58:25.89 RNAlater soak for 10:00 @17:08:25.92 Archive.seek :tVent @17:08:27.56 delay 1 second @17:08:28.58 Archive.seek :evac @17:08:44.53 delay 10 seconds @17:08:54.56 Sample preserved and evacuated @17:08:55.79 -> Cmd.stop @17:08:55.94 Carousel.seek 59 @17:08:56.13 Plunge.seek :locked @17:08:57.10 Archive.in :unlocking @17:08:57.22 Archive.seek :clear @17:08:57.34 Air.in :unlocking @17:08:57.42 Air.seek :clear @17:08:57.49 Lysis1.in :unlocking @17:08:57.62 Lysis1.seek :clear @17:09:01.27 Gate.power :motor,:OFF @17:09:01.29 Gate.power :core,:OFF @17:09:01.30 Gate.power :analytic1,:OFF @17:09:01.33 Gate.power :analytic2,:OFF @17:09:01.36 Gate.power :analytic3,:OFF @17:09:01.38 Gate.power :main,:OFF @17:09:01.39 Safely stopped and ready to power off ##-- file closed 2022-05-14T20:00:04.552Z