##-- file opened 2021-08-12T01:00:41.604Z @21:00:41.69 -> showStatus @21:00:43.70 -> Cmd.loadCartridge -4 @21:00:43.76 Gate.power :main,:ON,limit to 5000mA @21:00:43.77 Gate.power :analytic1,:ON,limit to 5000mA @21:00:43.80 Gate.power :analytic2,:ON,limit to 5000mA @21:00:43.82 Gate.power :analytic3,:ON,limit to 5000mA @21:00:43.96 Powering core bus @21:00:46.24 SPR Module 1, FW Rev. B, FPGA Rev. C @21:00:46.71 Archive.configure :default @21:00:46.84 Air.configure :default @21:00:46.94 Lysis1.configure :default @21:00:47.04 Lysis2.configure :default @21:00:47.17 Plunge.configure :default @21:00:47.29 SamplePump.configure :default @21:00:47.43 ExtraPump.configure :default @21:00:47.55 Toroid.configure :default @21:00:47.67 Carousel.configure :default @21:00:47.80 Pressure.configure :default @21:00:47.91 PinPort.configure :default @21:00:47.97 All elf controllers running firmware version 4.741 @21:00:48.00 Gate.power :motor,:ON,limit to 5000mA @21:00:48.22 Archive.in :default @21:00:48.48 Archive.home.jog 0 @21:00:48.63 Toroid.in :default @21:00:48.70 Plunge.in :default @21:00:48.74 Lysis1.in :default @21:00:48.78 Air.in :default @21:00:48.81 PinPort.in :default @21:00:48.90 Lysis2.in :default @21:00:49.03 Toroid.home.jog 0 @21:00:49.09 Plunge.home.jog 0 @21:00:49.11 Lysis1.home.jog 0 @21:00:49.16 Air.home.jog 0 @21:00:49.20 PinPort.home.jog 0 @21:00:49.24 Lysis2.home.jog 0 @21:00:49.69 Archive.in :homing @21:00:49.89 Toroid.in :homing @21:00:49.92 Plunge.in :homing @21:00:49.96 Lysis1.in :homing @21:00:49.99 Air.in :homing @21:00:50.04 Archive.jog 1392 @21:00:50.07 PinPort.in :homing @21:00:50.12 Lysis2.in :homing @21:00:50.30 Toroid.jog 620 @21:00:50.34 Lysis1.jog 1411 @21:00:50.38 Plunge.jog 1546 @21:00:50.41 PinPort.jog -109 @21:00:50.45 Air.jog 1412 @21:00:50.50 Lysis2.jog 1409 @21:00:50.80 PinPort.setRawPosition! 0 @21:00:50.84 PinPort.seek :home @21:00:50.93 PinPort.in :default @21:00:51.36 Archive.setRawPosition! 50 @21:00:51.40 Archive.seek :home @21:00:51.68 Lysis1.setRawPosition! 50 @21:00:51.71 Archive.in :default @21:00:51.81 Lysis1.seek :home @21:00:51.92 Plunge.setRawPosition! 50 @21:00:51.95 Lysis2.setRawPosition! 50 @21:00:51.97 Air.setRawPosition! 50 @21:00:52.06 Plunge.seek :home @21:00:52.15 Lysis2.seek :home @21:00:52.19 Air.seek :home @21:00:52.33 Lysis1.in :default @21:00:52.42 Plunge.in :default @21:00:52.54 Lysis2.in :default @21:00:52.57 Toroid.setRawPosition! 0 @21:00:52.60 Air.in :default @21:00:52.74 Toroid.seek :home @21:00:52.83 Toroid.in :default @21:00:53.05 Toroid.seek :clear @21:00:54.66 Carousel.in :default @21:00:54.72 Carousel.home.jog 0 @21:00:55.21 Carousel.in :default @21:00:55.27 Carousel.seek 55 @21:00:55.42 Selecting Cartridge 26 @21:00:55.67 Carousel.seek 26 @21:01:01.85 Lysis1.in :fast @21:01:01.91 Lysis1.seek :locked @21:01:04.51 Plunge.seek :locked @21:01:04.64 Air.in :fast @21:01:04.69 Air.seek :locked @21:01:07.32 Powering cartridge bus @21:01:09.94 Gate.setIR 1750,5000,0,6 @21:01:10.07 Gate.setIR 0,5000,0,6 @21:01:10.12 Gate.power :cartridge,:OFF @21:01:10.66 Mounted archive Cartridge @21:01:23.29 -> Cmd.startFiltering @21:01:23.74 Priming sample loop w/100ml, bypass w/10ml @21:01:23.76 SamplePump.setPosition! 0ml @21:01:23.80 Bypass.open @21:01:25.31 Intake.open @21:01:25.64 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:01:28.20 Vacuum tare is 51% of full scale @21:01:28.33 Pressure.in :default @21:01:28.39 Pressure.hold @21:01:28.56 SamplePump.in :prime @21:01:28.63 SamplePump.seek 10ml,59 seconds @21:01:37.89 Pressure.stop @21:01:37.94 SamplePump.setPosition! 0ml @21:01:37.99 Exhaust.open @21:01:39.42 Bypass.close @21:01:40.98 Pressure.in :default @21:01:41.04 Pressure.hold @21:01:41.25 SamplePump.in :prime @21:01:41.32 SamplePump.seek 100ml,5:16 @21:03:46.91 Pressure.stop @21:03:46.97 Bypass.open @21:03:47.67 Intake.close @21:03:48.22 Toroid.in :hold @21:03:48.28 Toroid.seek :crack @21:03:49.79 Toroid.seek :cartridge @21:03:50.88 Toroid.in :default @21:03:50.94 Toroid.seek :clear @21:03:52.90 Pressurizing cartridge to 26psi above 13.5psia @21:03:52.91 SamplePump.setPosition! 0ml @21:03:52.95 Intake.open @21:03:53.51 Bypass.close @21:03:54.22 Exhaust.close @21:03:55.02 Pressure.in :default @21:03:55.08 Pressure.hold @21:03:55.25 SamplePump.in :charge @21:03:55.32 SamplePump.seek 2.3ml,42 seconds @21:04:03.12 Pressure.stop @21:04:03.17 Intake.close @21:04:04.00 Pressurized cartridge with 0.269ml to 27.2psi @21:04:04.02 Isolated @34.0psia (20.5psi) @21:04:04.03 Verifying cartridge holds at least 0.75..0.85 of 34.0psia @21:04:04.07 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @21:04:08.69 Sampling 1000.0ml @21:04:08.71 SamplePump.setPosition! 0ml @21:04:08.76 Exhaust.open @21:04:09.48 Intake.open @21:04:10.32 Pressure.in :default @21:04:10.38 Pressure.hold @21:04:10.60 SamplePump.in :sample @21:04:10.67 SamplePump.seek 1000ml,1:23:53 @21:04:13.36 Cartridge 26 filtering 1/1000ml at 0.39ml/s with 24.0psi vacuum @21:05:13.35 Cartridge 26 filtering 23/1000ml at 0.39ml/s with 23.5psi vacuum @21:06:13.36 Cartridge 26 filtering 44/1000ml at 0.32ml/s with 22.3psi vacuum @21:07:13.35 Cartridge 26 filtering 61/1000ml at 0.25ml/s with 21.1psi vacuum @21:08:13.36 Cartridge 26 filtering 76/1000ml at 0.20ml/s with 24.3psi vacuum @21:09:13.35 Cartridge 26 filtering 90/1000ml at 0.21ml/s with 21.8psi vacuum @21:10:13.36 Cartridge 26 filtering 105/1000ml at 0.29ml/s with 25.5psi vacuum @21:11:13.35 Cartridge 26 filtering 120/1000ml at 0.22ml/s with 24.2psi vacuum @21:12:13.36 Cartridge 26 filtering 136/1000ml at 0.21ml/s with 22.7psi vacuum @21:13:13.35 Cartridge 26 filtering 150/1000ml at 0.22ml/s with 20.3psi vacuum @21:14:13.36 Cartridge 26 filtering 163/1000ml at 0.22ml/s with 23.8psi vacuum @21:15:13.35 Cartridge 26 filtering 176/1000ml at 0.20ml/s with 22.8psi vacuum @21:16:13.36 Cartridge 26 filtering 188/1000ml at 0.20ml/s with 25.4psi vacuum @21:16:18.26 Flow averaged 0.26ml/s over 12:08 @21:16:18.29 Waiting up to 16 seconds for vacuum to reduce from 29.2 to 18.0psi @21:16:18.87 Relieved excess vacuum in .6 seconds @21:16:18.90 SamplePump.seek 1000ml,1:08:06 @21:16:31.75 Flow averaged 0.20ml/s over 13 seconds @21:16:31.77 Intake.close @21:16:31.81 Exhaust.close @21:16:32.02 Toroid.in :hold @21:16:32.08 Toroid.seek :bypass @21:16:33.71 Toroid.in :default @21:16:33.77 Toroid.seek :clear @21:16:35.38 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @21:16:35.39 Pressure.stop @21:16:35.57 Sampled 191.9ml @21:16:37.60 -> Cmd.startProcessing @21:16:37.97 Plunge.seek :reagent @21:16:38.80 Sample loop closed. Ready to Deliver Reagents @21:16:38.81 Archive.seek :sealed @21:16:43.08 Archive.seek :evac @21:16:57.01 delay 1:00 @21:17:57.04 Archive.seek :clear @21:18:14.74 RNAlater soak for 10:00 @21:28:14.76 Archive.seek :tVent @21:28:17.34 delay 1 second @21:28:18.36 Archive.seek :evac @21:28:33.98 delay 10 seconds @21:28:44.01 Archive.seek :tVent @21:28:59.64 delay 1 second @21:29:00.66 Archive.seek :evac @21:29:16.29 delay 10 seconds @21:29:26.32 Sample preserved and evacuated @21:29:27.28 -> Cmd.stop @21:29:27.42 Carousel.seek 26 @21:29:27.59 Plunge.seek :locked @21:29:28.54 Archive.in :unlocking @21:29:28.65 Archive.seek :clear @21:29:28.73 Air.in :unlocking @21:29:28.84 Air.seek :clear @21:29:28.92 Lysis1.in :unlocking @21:29:29.05 Lysis1.seek :clear @21:29:32.79 Gate.power :motor,:OFF @21:29:32.81 Gate.power :core,:OFF @21:29:32.83 Gate.power :analytic1,:OFF @21:29:32.85 Gate.power :analytic2,:OFF @21:29:32.87 Gate.power :analytic3,:OFF @21:29:32.89 Gate.power :main,:OFF @21:29:32.91 Safely stopped and ready to power off ##-- file closed 2021-08-12T01:38:30.648Z ##-- file opened 2021-08-12T01:38:30.649Z @21:38:30.73 -> showStatus @21:38:32.75 -> Cmd.loadCartridge -18 @21:38:32.81 Gate.power :main,:ON,limit to 5000mA @21:38:32.83 Gate.power :analytic1,:ON,limit to 5000mA @21:38:32.85 Gate.power :analytic2,:ON,limit to 5000mA @21:38:32.87 Gate.power :analytic3,:ON,limit to 5000mA @21:38:33.01 Powering core bus @21:38:35.29 SPR Module 1, FW Rev. B, FPGA Rev. C @21:38:35.76 Archive.configure :default @21:38:35.89 Air.configure :default @21:38:35.99 Lysis1.configure :default @21:38:36.09 Lysis2.configure :default @21:38:36.21 Plunge.configure :default @21:38:36.34 SamplePump.configure :default @21:38:36.48 ExtraPump.configure :default @21:38:36.59 Toroid.configure :default @21:38:36.72 Carousel.configure :default @21:38:36.85 Pressure.configure :default @21:38:36.97 PinPort.configure :default @21:38:37.03 All elf controllers running firmware version 4.741 @21:38:37.05 Gate.power :motor,:ON,limit to 5000mA @21:38:37.20 Archive.in :default @21:38:37.52 Archive.home.jog 0 @21:38:37.67 Toroid.in :default @21:38:37.75 Plunge.in :default @21:38:37.78 Lysis1.in :default @21:38:37.82 Air.in :default @21:38:37.86 PinPort.in :default @21:38:37.89 Lysis2.in :default @21:38:38.10 Toroid.home.jog 0 @21:38:38.12 Plunge.home.jog 0 @21:38:38.18 Lysis1.home.jog 0 @21:38:38.22 Air.home.jog 0 @21:38:38.26 PinPort.home.jog 0 @21:38:38.29 Lysis2.home.jog 0 @21:38:38.64 Archive.in :homing @21:38:38.91 Toroid.in :homing @21:38:38.95 Plunge.in :homing @21:38:38.98 Lysis1.in :homing @21:38:39.03 Archive.jog 184 @21:38:39.06 PinPort.in :homing @21:38:39.09 Lysis2.in :homing @21:38:39.13 Air.in :homing @21:38:39.32 Plunge.jog 1564 @21:38:39.39 Toroid.jog 629 @21:38:39.42 PinPort.jog -108 @21:38:39.46 Lysis2.jog 1408 @21:38:39.50 Lysis1.jog 1413 @21:38:39.53 Air.jog 1410 @21:38:39.74 Archive.setRawPosition! 50 @21:38:39.79 Archive.seek :home @21:38:39.87 PinPort.setRawPosition! 0 @21:38:39.92 PinPort.seek :home @21:38:40.00 PinPort.in :default @21:38:40.09 Archive.in :default @21:38:40.65 Plunge.setRawPosition! 50 @21:38:40.70 Plunge.seek :home @21:38:40.81 Lysis1.setRawPosition! 50 @21:38:40.85 Lysis1.seek :home @21:38:40.94 Lysis2.setRawPosition! 50 @21:38:40.97 Air.setRawPosition! 50 @21:38:41.05 Lysis2.seek :home @21:38:41.08 Air.seek :home @21:38:41.22 Lysis1.in :default @21:38:41.25 Plunge.in :default @21:38:41.41 Lysis2.in :default @21:38:41.48 Air.in :default @21:38:41.71 Toroid.setRawPosition! 0 @21:38:41.76 Toroid.seek :home @21:38:41.84 Toroid.in :default @21:38:41.98 Toroid.seek :clear @21:38:43.60 Carousel.in :default @21:38:43.66 Carousel.home.jog 0 @21:38:44.15 Carousel.in :default @21:38:44.21 Carousel.seek 26 @21:38:44.39 Selecting Cartridge 23 @21:38:44.63 Carousel.seek 23 @21:38:45.51 Lysis1.in :fast @21:38:45.58 Lysis1.seek :locked @21:38:48.17 Plunge.seek :locked @21:38:48.29 Air.in :fast @21:38:48.35 Air.seek :locked @21:38:50.95 Powering cartridge bus @21:38:53.58 Gate.setIR 1750,5000,0,6 @21:38:53.71 Gate.setIR 0,5000,0,6 @21:38:53.77 PuckHeater.configure :default @21:38:53.82 Gate.power :heat,:ON,limit to 5000mA @21:38:54.52 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05084 @21:38:54.55 Using default puck heat loss curve for HeatingAccelInCarousel @21:39:13.14 -> Cmd.startFiltering @21:39:13.60 Priming sample loop w/100ml, bypass w/10ml @21:39:13.61 SamplePump.setPosition! 0ml @21:39:13.65 Bypass.open @21:39:15.14 Intake.open @21:39:15.39 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:39:17.95 Vacuum tare is 51% of full scale @21:39:18.14 Pressure.in :default @21:39:18.21 Pressure.hold @21:39:18.37 SamplePump.in :prime @21:39:18.44 SamplePump.seek 10ml,59 seconds @21:39:27.70 Pressure.stop @21:39:27.75 SamplePump.setPosition! 0ml @21:39:27.80 Exhaust.open @21:39:29.22 Bypass.close @21:39:30.81 Pressure.in :default @21:39:30.87 Pressure.hold @21:39:31.09 SamplePump.in :prime @21:39:31.15 SamplePump.seek 100ml,5:16 @21:41:36.54 Pressure.stop @21:41:36.60 Bypass.open @21:41:37.33 Intake.close @21:41:38.17 Toroid.in :hold @21:41:38.23 Toroid.seek :crack @21:41:39.74 Toroid.seek :cartridge @21:41:40.82 Toroid.in :default @21:41:40.88 Toroid.seek :clear @21:41:43.24 Pressurizing cartridge to 14psi above 12.8psia @21:41:43.25 SamplePump.setPosition! 0ml @21:41:43.30 Intake.open @21:41:44.02 Bypass.close @21:41:44.77 Exhaust.close @21:41:45.58 Pressure.in :default @21:41:45.64 Pressure.hold @21:41:45.81 SamplePump.in :charge @21:41:45.88 SamplePump.seek 2.3ml,41 seconds @21:41:56.75 Pressure.stop @21:41:56.80 Intake.close @21:41:57.63 Pressurized cartridge with 0.527ml to 14.2psi @21:41:57.64 Isolated @23.0psia (10.2psi) @21:41:57.66 Verifying cartridge holds at least 0.75..0.85 of 23.0psia @21:41:57.69 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @21:42:05.85 Toroid.in :hold @21:42:05.91 Toroid.seek :bypass @21:42:07.53 Toroid.in :default @21:42:07.59 Toroid.seek :clear @21:42:09.25 Cartridge::Sampler::Leaky in FILTERING -- Isolated cartridge pressure fell by 25% to 17.1psia @21:42:09.27 Retry #1 of 2 @21:42:09.29 Cmd::SpareCartridge in FILTERING -- 0 microSPR_phyto spares remaining @21:42:09.34 Selecting Cartridge 21 @21:42:09.47 Gate.power :heat,:OFF @21:42:09.49 Gate.power :cartridge,:OFF @21:42:09.74 Air.in :unlocking @21:42:09.86 Air.seek :clear @21:42:11.25 Lysis1.in :unlocking @21:42:11.37 Lysis1.seek :clear @21:42:11.45 Plunge.in :unlocking @21:42:11.51 Plunge.seek :clear @21:42:12.70 Carousel.seek 21 @21:42:13.40 Lysis1.in :fast @21:42:13.46 Lysis1.seek :locked @21:42:16.05 Plunge.in :default @21:42:16.16 Plunge.seek :locked @21:42:16.24 Air.in :fast @21:42:16.30 Air.seek :locked @21:42:18.90 Powering cartridge bus @21:42:21.52 Gate.setIR 1750,5000,0,6 @21:42:21.65 Gate.setIR 0,5000,0,6 @21:42:21.71 PuckHeater.configure :default @21:42:21.76 Gate.power :heat,:ON,limit to 5000mA @21:42:22.45 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05040 @21:42:22.48 Using default puck heat loss curve for HeatingAccelInCarousel @21:42:22.68 Bypass.open @21:42:23.51 Toroid.in :hold @21:42:23.57 Toroid.seek :crack @21:42:25.10 Toroid.seek :cartridge @21:42:26.20 Toroid.in :default @21:42:26.26 Toroid.seek :clear @21:42:28.05 Exhaust.open @21:42:28.92 Pressurizing cartridge to 14psi above 13.0psia @21:42:28.94 SamplePump.setPosition! 0ml @21:42:28.98 Intake.open @21:42:29.72 Bypass.close @21:42:30.48 Exhaust.close @21:42:31.27 Pressure.in :default @21:42:31.33 Pressure.hold @21:42:31.51 SamplePump.seek 2.3ml,41 seconds @21:42:39.90 Pressure.stop @21:42:39.95 Intake.close @21:42:40.79 Pressurized cartridge with 0.312ml to 14.9psi @21:42:40.80 Isolated @23.9psia (10.9psi) @21:42:40.82 Verifying cartridge holds at least 0.75..0.85 of 23.9psia @21:42:40.85 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @21:42:45.47 Sampling 75.0ml @21:42:45.49 SamplePump.setPosition! 0ml @21:42:45.54 Exhaust.open @21:42:46.25 Intake.open @21:42:47.14 Pressure.in :default @21:42:47.20 Pressure.hold @21:42:47.44 SamplePump.in :sample @21:42:47.51 SamplePump.seek 75ml,5:31 @21:42:50.17 Cartridge 21 filtering 1/75ml at 0.31ml/s with 13.1psi vacuum @21:43:50.18 Cartridge 21 filtering 20/75ml at 0.32ml/s with 11.4psi vacuum @21:44:50.17 Cartridge 21 filtering 39/75ml at 0.33ml/s with 13.3psi vacuum @21:45:50.18 Cartridge 21 filtering 58/75ml at 0.31ml/s with 13.9psi vacuum @21:46:45.28 Pressure.stop @21:46:45.40 Intake.close @21:46:45.45 Exhaust.close @21:46:45.60 Toroid.in :hold @21:46:45.66 Toroid.seek :bypass @21:46:47.29 Toroid.in :default @21:46:47.35 Toroid.seek :clear @21:46:48.96 Sampled 75.0ml @21:46:50.51 -> Cmd.startProcessing @21:46:52.04 SPR.command \"read_sequences\" @21:46:52.68 SPR.command \"stats_on\" @21:46:52.72 SPR.command \"start 0 1\" @21:46:53.53 SPR.start /var/log/esp/spr21aug1121h.spr.bz2 @21:46:55.53 Handoff.select :waste @21:47:02.22 Syringe tare is 19% of full scale @21:47:02.71 PinPort.in :locking @21:47:02.78 PinPort.seek :locked @21:47:03.26 Archive.in :fast @21:47:03.30 Lysis2.in :fast @21:47:03.38 Lysis1.seek 1350uL @21:47:03.44 Archive.seek :full @21:47:03.51 Lysis2.seek :full @21:47:05.64 Plunge.seek :reagent @21:47:06.55 Evacuating puck with filter bubble point of 24psi @21:47:06.57 PinPort.seek :locked @21:47:06.65 Handoff.select :waste @21:47:13.24 Air syringe pumping up to 1 stroke to reach 8psi @21:47:13.25 Air stroke #1 of 1 @21:47:13.34 Air.in :pressurize @21:47:13.40 Air.seek :pressurized @21:47:24.64 -0.1psi after pressurize @21:47:24.65 Air.in :compress @21:47:24.73 Air.seek :evac @21:47:28.81 -0.0psi after compress @21:47:41.38 -0.1psi after compress (stable) @21:47:41.41 Air.in :default @21:47:41.49 Air.hold @21:47:41.66 at -0.1psi Opened to waste @21:47:41.67 Handoff.select :blocked @21:47:41.80 Archive syringe pumping up to 8 strokes to reach 24psi @21:47:41.81 Archive stroke #1 of 8 @21:47:41.89 Archive.in :pressurize @21:47:41.95 Archive.seek :pressurized @21:47:54.72 -0.1psi after pressurize @21:47:54.73 Archive.in :compress @21:47:54.80 Archive.seek :empty @21:48:10.68 -0.1psi after compress @21:48:23.26 -0.2psi after compress (stable) @21:48:23.28 Archive.in :gulp @21:48:23.35 Archive.seek :topVent @21:48:30.55 -0.1psi after gulp @21:48:43.13 -0.1psi after gulp (stable) @21:48:43.14 Archive stroke #2 of 8 @21:48:43.22 Archive.in :pressurize @21:48:43.28 Archive.seek :pressurized @21:48:56.01 -0.1psi after pressurize @21:48:56.02 Archive.in :compress @21:48:56.09 Archive.seek :empty @21:49:11.96 -0.0psi after compress @21:49:24.55 0.1psi after compress (stable) @21:49:24.57 Archive.in :gulp @21:49:24.65 Archive.seek :topVent @21:49:31.85 0.8psi after gulp @21:49:44.43 1.0psi after gulp (stable) @21:49:44.44 Archive stroke #3 of 8 @21:49:44.53 Archive.in :pressurize @21:49:44.59 Archive.seek :pressurized @21:49:57.32 1.0psi after pressurize @21:49:57.33 Archive.in :compress @21:49:57.41 Archive.seek :empty @21:50:09.57 Archive.in :default @21:50:09.63 Archive.hold @21:50:09.69 Handoff.select :blocked @21:50:09.71 SPR.command \"start 2 14\" @21:50:10.89 SPR stabilized @21:50:11.62 Ended log spr21aug1121h.spr.bz2 @21:50:11.63 SPR.command \"stats_off\" @21:50:12.58 Archive.setRawPosition! -7996 @21:50:12.63 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 78uL (sensed 103uL) @21:50:12.65 Thread::ChildDied in LRAUV -- :PROCESSING @21:50:16.14 -> Cmd.stop @21:50:16.22 Gate.power :heat,:OFF @21:50:16.24 Gate.power :cartridge,:OFF @21:50:16.33 Carousel.seek 21 @21:50:16.51 Plunge.seek :locked @21:50:17.39 PinPort.in :unlocking @21:50:17.45 PinPort.seek :clear @21:50:17.99 Archive.in :unlocking @21:50:18.10 Archive.seek :clear @21:50:18.19 Air.in :unlocking @21:50:18.31 Lysis1.in :unlocking @21:50:18.35 Air.seek :clear @21:50:18.51 Lysis1.seek :clear @21:50:18.57 Lysis2.in :unlocking @21:50:18.65 Lysis2.seek :clear @21:50:22.14 Slide::Error in LRAUV -- Archive Syringe (ArS) positionErr at 958uL (sensed 933uL) @21:50:22.15 Retry #1 of 2 @21:50:22.31 Gate.power :motor,:OFF @21:50:22.33 Gate.power :core,:OFF @21:50:22.35 Gate.power :analytic1,:OFF @21:50:22.37 Gate.power :analytic2,:OFF @21:50:22.40 Gate.power :analytic3,:OFF @21:50:22.42 Gate.power :main,:OFF @21:50:26.16 Gate.power :main,:ON,limit to 5000mA @21:50:26.17 Gate.power :analytic1,:ON,limit to 5000mA @21:50:26.20 Gate.power :analytic2,:ON,limit to 5000mA @21:50:26.22 Gate.power :analytic3,:ON,limit to 5000mA @21:50:26.28 Powering core bus @21:50:28.66 SPR Module 1, FW Rev. B, FPGA Rev. C @21:50:29.02 Archive.configure :default @21:50:29.14 Air.configure :default @21:50:29.25 Lysis1.configure :default @21:50:29.35 Lysis2.configure :default @21:50:29.47 Plunge.configure :default @21:50:29.59 SamplePump.configure :default @21:50:29.74 ExtraPump.configure :default @21:50:29.84 Toroid.configure :default @21:50:29.97 Carousel.configure :default @21:50:30.10 Pressure.configure :default @21:50:30.22 PinPort.configure :default @21:50:30.28 All elf controllers running firmware version 4.741 @21:50:30.30 Gate.power :motor,:ON,limit to 5000mA @21:50:30.38 Archive.setRawPosition! -3829 @21:50:30.47 Air.setRawPosition! 5 @21:50:30.56 Lysis1.setRawPosition! 17 @21:50:30.66 Lysis2.setRawPosition! 26 @21:50:30.75 Plunge.setRawPosition! -1274 @21:50:30.84 Toroid.setRawPosition! -478 @21:50:30.94 Carousel.setRawPosition! 5001 @21:50:31.03 PinPort.setRawPosition! -1 @21:50:31.20 Carousel.seek 21 @21:50:31.40 Lysis1.in :fast @21:50:31.46 Lysis1.seek :locked @21:50:34.01 Plunge.seek :locked @21:50:34.35 Archive.in :unlocking @21:50:34.47 Archive.seek :clear @21:50:34.54 Air.in :unlocking @21:50:34.67 Air.seek :clear @21:50:34.76 Lysis1.in :unlocking @21:50:34.89 Lysis1.seek :clear @21:50:34.96 Lysis2.in :unlocking @21:50:35.04 Lysis2.seek :clear @21:50:36.78 Gate.power :motor,:OFF @21:50:36.80 Gate.power :core,:OFF @21:50:36.82 Gate.power :analytic1,:OFF @21:50:36.84 Gate.power :analytic2,:OFF @21:50:36.86 Gate.power :analytic3,:OFF @21:50:36.88 Gate.power :main,:OFF @21:50:36.90 Safely stopped and ready to power off ##-- file closed 2021-08-12T03:55:45.830Z ##-- file opened 2021-08-12T03:55:45.834Z @23:55:45.97 -> showStatus @23:55:47.99 -> Cmd.loadCartridge -4 @23:55:48.05 Gate.power :main,:ON,limit to 5000mA @23:55:48.06 Gate.power :analytic1,:ON,limit to 5000mA @23:55:48.09 Gate.power :analytic2,:ON,limit to 5000mA @23:55:48.11 Gate.power :analytic3,:ON,limit to 5000mA @23:55:48.25 Powering core bus @23:55:50.53 SPR Module 1, FW Rev. B, FPGA Rev. C @23:55:50.99 Archive.configure :default @23:55:51.12 Air.configure :default @23:55:51.22 Lysis1.configure :default @23:55:51.33 Lysis2.configure :default @23:55:51.45 Plunge.configure :default @23:55:51.57 SamplePump.configure :default @23:55:51.72 ExtraPump.configure :default @23:55:51.83 Toroid.configure :default @23:55:51.95 Carousel.configure :default @23:55:52.07 Pressure.configure :default @23:55:52.19 PinPort.configure :default @23:55:52.25 All elf controllers running firmware version 4.741 @23:55:52.28 Gate.power :motor,:ON,limit to 5000mA @23:55:52.45 Archive.in :default @23:55:52.73 Archive.home.jog 0 @23:55:52.88 Toroid.in :default @23:55:52.96 Plunge.in :default @23:55:52.99 Lysis1.in :default @23:55:53.04 PinPort.in :default @23:55:53.10 Lysis2.in :default @23:55:53.13 Air.in :default @23:55:53.31 Toroid.home.jog 0 @23:55:53.35 Plunge.home.jog 0 @23:55:53.38 Lysis1.home.jog 0 @23:55:53.40 PinPort.home.jog 0 @23:55:53.43 Air.home.jog 0 @23:55:53.45 Lysis2.home.jog 0 @23:55:53.94 Archive.in :homing @23:55:54.17 Toroid.in :homing @23:55:54.20 Plunge.in :homing @23:55:54.24 Lysis1.in :homing @23:55:54.27 Archive.jog 1367 @23:55:54.30 PinPort.in :homing @23:55:54.33 Lysis2.in :homing @23:55:54.39 Air.in :homing @23:55:54.55 Toroid.jog 626 @23:55:54.60 Plunge.jog 1538 @23:55:54.63 PinPort.jog -108 @23:55:54.66 Lysis1.jog 150 @23:55:54.70 Lysis2.jog 151 @23:55:54.75 Air.jog 1415 @23:55:55.01 PinPort.setRawPosition! 0 @23:55:55.06 PinPort.seek :home @23:55:55.14 PinPort.in :default @23:55:55.28 Lysis1.setRawPosition! 50 @23:55:55.32 Lysis1.seek :home @23:55:55.40 Lysis2.setRawPosition! 50 @23:55:55.45 Lysis2.seek :home @23:55:55.60 Archive.setRawPosition! 50 @23:55:55.64 Archive.seek :home @23:55:55.74 Lysis2.in :default @23:55:55.77 Lysis1.in :default @23:55:55.92 Archive.in :default @23:55:56.04 Plunge.setRawPosition! 50 @23:55:56.06 Air.setRawPosition! 50 @23:55:56.13 Plunge.seek :home @23:55:56.18 Air.seek :home @23:55:56.47 Plunge.in :default @23:55:56.56 Air.in :default @23:55:56.85 Toroid.setRawPosition! 0 @23:55:56.90 Toroid.seek :home @23:55:56.99 Toroid.in :default @23:55:57.12 Toroid.seek :clear @23:55:58.73 Carousel.in :default @23:55:58.79 Carousel.home.jog 0 @23:55:59.27 Carousel.in :default @23:55:59.33 Carousel.seek 21 @23:55:59.46 Selecting Cartridge 24 @23:55:59.71 Carousel.seek 24 @23:56:00.59 Lysis1.in :fast @23:56:00.66 Lysis1.seek :locked @23:56:03.25 Plunge.seek :locked @23:56:03.37 Air.in :fast @23:56:03.43 Air.seek :locked @23:56:06.03 Powering cartridge bus @23:56:08.65 Gate.setIR 1750,5000,0,6 @23:56:08.78 Gate.setIR 0,5000,0,6 @23:56:08.83 Gate.power :cartridge,:OFF @23:56:09.37 Mounted archive Cartridge @23:56:27.58 -> Cmd.startFiltering @23:56:28.03 Priming sample loop w/100ml, bypass w/10ml @23:56:28.04 SamplePump.setPosition! 0ml @23:56:28.09 Bypass.open @23:56:29.56 Intake.open @23:56:30.37 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @23:56:32.94 Vacuum tare is 51% of full scale @23:56:33.13 Pressure.in :default @23:56:33.19 Pressure.hold @23:56:33.36 SamplePump.in :prime @23:56:33.43 SamplePump.seek 10ml,59 seconds @23:56:42.69 Pressure.stop @23:56:42.74 SamplePump.setPosition! 0ml @23:56:42.79 Exhaust.open @23:56:44.24 Bypass.close @23:56:45.77 Pressure.hold @23:56:45.99 SamplePump.in :prime @23:56:46.07 SamplePump.seek 100ml,5:16 @23:58:51.65 Pressure.stop @23:58:51.71 Bypass.open @23:58:52.43 Intake.close @23:58:53.29 Toroid.in :hold @23:58:53.35 Toroid.seek :crack @23:58:54.86 Toroid.seek :cartridge @23:58:55.95 Toroid.in :default @23:58:56.01 Toroid.seek :clear @23:58:58.35 Pressurizing cartridge to 26psi above 13.2psia @23:58:58.37 SamplePump.setPosition! 0ml @23:58:58.41 Intake.open @23:58:59.15 Bypass.close @23:58:59.90 Exhaust.close @23:59:00.73 Pressure.in :default @23:59:00.79 Pressure.hold @23:59:00.97 SamplePump.in :charge @23:59:01.03 SamplePump.seek 2.3ml,42 seconds @23:59:08.69 Pressure.stop @23:59:08.74 Intake.close @23:59:09.03 Pressurized cartridge with 0.260ml to 26.0psi @23:59:09.04 Isolated @34.5psia (21.3psi) @23:59:09.05 Verifying cartridge holds at least 0.75..0.85 of 34.5psia @23:59:09.08 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @23:59:13.65 Cartridge pressure fell 2% to 33.9psia @23:59:13.74 Sampling 1000.0ml @23:59:13.75 SamplePump.setPosition! 0ml @23:59:13.80 Exhaust.open @23:59:14.53 Intake.open @23:59:15.44 Pressure.in :default @23:59:15.50 Pressure.hold @23:59:15.72 SamplePump.in :sample @23:59:15.80 SamplePump.seek 1000ml,1:23:53 @23:59:18.47 Cartridge 24 filtering 1/1000ml at 0.36ml/s with 23.1psi vacuum @00:00:18.47EDT12-Aug-21 Cartridge 24 filtering 23/1000ml at 0.34ml/s with 25.9psi vacuum @00:01:18.48 Cartridge 24 filtering 45/1000ml at 0.33ml/s with 23.4psi vacuum @00:02:18.47 Cartridge 24 filtering 65/1000ml at 0.36ml/s with 23.0psi vacuum @00:03:18.48 Cartridge 24 filtering 84/1000ml at 0.34ml/s with 24.2psi vacuum @00:04:18.47 Cartridge 24 filtering 101/1000ml at 0.31ml/s with 21.0psi vacuum @00:05:18.48 Cartridge 24 filtering 118/1000ml at 0.28ml/s with 22.4psi vacuum @00:06:18.47 Cartridge 24 filtering 133/1000ml at 0.28ml/s with 24.0psi vacuum @00:07:18.48 Cartridge 24 filtering 147/1000ml at 0.25ml/s with 22.8psi vacuum @00:08:18.47 Cartridge 24 filtering 160/1000ml at 0.22ml/s with 23.9psi vacuum @00:09:18.48 Cartridge 24 filtering 173/1000ml at 0.20ml/s with 27.9psi vacuum @00:10:08.04 Flow averaged 0.28ml/s over 10:53 @00:10:08.07 Waiting up to 15 seconds for vacuum to reduce from 28.9 to 18.0psi @00:10:08.65 Relieved excess vacuum in .6 seconds @00:10:08.67 SamplePump.seek 1000ml,1:08:37 @00:10:18.47 Cartridge 24 filtering 185/1000ml at 0.20ml/s with 23.6psi vacuum @00:10:25.30 Flow averaged 0.20ml/s over 17 seconds @00:10:25.33 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @00:10:26.15 Relieved excess vacuum in .8 seconds @00:10:26.17 SamplePump.seek 1000ml,1:08:20 @00:10:38.25 Flow averaged 0.20ml/s over 13 seconds @00:10:38.28 Waiting up to 18 seconds for vacuum to reduce from 29.8 to 18.0psi @00:10:39.10 Relieved excess vacuum in .9 seconds @00:10:39.13 SamplePump.seek 1000ml,1:08:08 @00:10:40.94 Flow estimate reduced to 0.16ml/s @00:10:40.97 Intake.close @00:10:41.04 Exhaust.close @00:10:41.19 Toroid.in :hold @00:10:41.26 Toroid.seek :bypass @00:10:42.89 Toroid.in :default @00:10:42.95 Toroid.seek :clear @00:10:44.57 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @00:10:44.58 Pressure.stop @00:10:44.77 Sampled 189.2ml @00:10:46.96 -> Cmd.startProcessing @00:10:47.33 Plunge.seek :reagent @00:10:48.17 Sample loop closed. Ready to Deliver Reagents @00:10:48.18 Archive.seek :sealed @00:10:52.44 Archive.seek :evac @00:11:06.37 delay 1:00 @00:12:06.39 Archive.seek :clear @00:12:24.11 RNAlater soak for 10:00 @00:22:24.13 Archive.seek :tVent @00:22:26.71 delay 1 second @00:22:27.73 Archive.seek :evac @00:22:43.35 delay 10 seconds @00:22:53.38 Archive.seek :tVent @00:23:09.00 delay 1 second @00:23:10.02 Archive.seek :evac @00:23:25.64 delay 10 seconds @00:23:35.67 Sample preserved and evacuated @00:23:36.62 -> Cmd.stop @00:23:36.76 Carousel.seek 24 @00:23:36.93 Plunge.seek :locked @00:23:37.88 Archive.in :unlocking @00:23:38.01 Archive.seek :clear @00:23:38.08 Air.in :unlocking @00:23:38.19 Air.seek :clear @00:23:38.28 Lysis1.in :unlocking @00:23:38.40 Lysis1.seek :clear @00:23:42.18 Gate.power :motor,:OFF @00:23:42.20 Gate.power :core,:OFF @00:23:42.22 Gate.power :analytic1,:OFF @00:23:42.24 Gate.power :analytic2,:OFF @00:23:42.26 Gate.power :analytic3,:OFF @00:23:42.28 Gate.power :main,:OFF @00:23:42.30 Safely stopped and ready to power off ##-- file closed 2021-08-12T04:32:41.040Z ##-- file opened 2021-08-12T04:32:41.042Z @00:32:40.88 -> showStatus @00:32:42.90 -> Cmd.loadCartridge -18 @00:32:42.95 Gate.power :main,:ON,limit to 5000mA @00:32:42.97 Gate.power :analytic1,:ON,limit to 5000mA @00:32:43.00 Gate.power :analytic2,:ON,limit to 5000mA @00:32:43.02 Gate.power :analytic3,:ON,limit to 5000mA @00:32:43.15 Powering core bus @00:32:45.44 SPR Module 1, FW Rev. B, FPGA Rev. C @00:32:45.89 Archive.configure :default @00:32:46.01 Air.configure :default @00:32:46.12 Lysis1.configure :default @00:32:46.23 Lysis2.configure :default @00:32:46.35 Plunge.configure :default @00:32:46.47 SamplePump.configure :default @00:32:46.62 ExtraPump.configure :default @00:32:46.73 Toroid.configure :default @00:32:46.85 Carousel.configure :default @00:32:46.98 Pressure.configure :default @00:32:47.09 PinPort.configure :default @00:32:47.15 All elf controllers running firmware version 4.741 @00:32:47.17 Gate.power :motor,:ON,limit to 5000mA @00:32:47.38 Archive.in :default @00:32:47.59 Archive.home.jog 0 @00:32:47.73 Toroid.in :default @00:32:47.80 Plunge.in :default @00:32:47.84 Lysis1.in :default @00:32:47.87 Air.in :default @00:32:47.92 PinPort.in :default @00:32:48.03 Lysis2.in :default @00:32:48.14 Toroid.home.jog 0 @00:32:48.19 Lysis1.home.jog 0 @00:32:48.24 Plunge.home.jog 0 @00:32:48.27 PinPort.home.jog 0 @00:32:48.31 Air.home.jog 0 @00:32:48.38 Lysis2.home.jog 0 @00:32:48.77 Archive.in :homing @00:32:48.99 Toroid.in :homing @00:32:49.03 Plunge.in :homing @00:32:49.06 Lysis1.in :homing @00:32:49.10 Archive.jog 1399 @00:32:49.13 PinPort.in :homing @00:32:49.16 Lysis2.in :homing @00:32:49.20 Air.in :homing @00:32:49.40 Toroid.jog 620 @00:32:49.45 Plunge.jog 1559 @00:32:49.47 PinPort.jog -108 @00:32:49.50 Lysis1.jog 1411 @00:32:49.53 Lysis2.jog 1410 @00:32:49.58 Air.jog 1407 @00:32:49.86 PinPort.setRawPosition! 0 @00:32:49.90 PinPort.seek :home @00:32:49.99 PinPort.in :default @00:32:50.43 Archive.setRawPosition! 50 @00:32:50.49 Archive.seek :home @00:32:50.76 Archive.in :default @00:32:50.89 Lysis2.setRawPosition! 50 @00:32:50.92 Air.setRawPosition! 50 @00:32:50.94 Plunge.setRawPosition! 50 @00:32:50.98 Lysis1.setRawPosition! 50 @00:32:51.07 Lysis2.seek :home @00:32:51.16 Air.seek :home @00:32:51.19 Plunge.seek :home @00:32:51.24 Lysis1.seek :home @00:32:51.44 Lysis2.in :default @00:32:51.55 Air.in :default @00:32:51.65 Toroid.setRawPosition! 0 @00:32:51.68 Plunge.in :default @00:32:51.71 Lysis1.in :default @00:32:51.82 Toroid.seek :home @00:32:51.96 Toroid.in :default @00:32:52.22 Toroid.seek :clear @00:32:53.83 Carousel.in :default @00:32:53.89 Carousel.home.jog 0 @00:32:54.39 Carousel.in :default @00:32:54.45 Carousel.seek 24 @00:32:54.59 Selecting Cartridge 19 @00:32:54.83 Carousel.seek 19 @00:32:56.12 Lysis1.in :fast @00:32:56.19 Lysis1.seek :locked @00:32:58.79 Plunge.seek :locked @00:32:58.91 Air.in :fast @00:32:58.97 Air.seek :locked @00:33:01.57 Powering cartridge bus @00:33:04.19 Gate.setIR 1750,5000,0,6 @00:33:04.32 Gate.setIR 0,5000,0,6 @00:33:04.38 PuckHeater.configure :default @00:33:04.44 Gate.power :heat,:ON,limit to 5000mA @00:33:05.10 Mounted lysis Cartridge board#Q05058 @00:33:05.13 Using default puck heat loss curve for HeatingAccelInCarousel @00:33:23.32 -> Cmd.startFiltering @00:33:23.78 Priming sample loop w/100ml, bypass w/10ml @00:33:23.80 SamplePump.setPosition! 0ml @00:33:23.84 Bypass.open @00:33:25.34 Intake.open @00:33:26.13 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @00:33:28.71 Vacuum tare is 51% of full scale @00:33:28.83 Pressure.in :default @00:33:28.89 Pressure.hold @00:33:29.06 SamplePump.in :prime @00:33:29.13 SamplePump.seek 10ml,59 seconds @00:33:38.40 Pressure.stop @00:33:38.46 SamplePump.setPosition! 0ml @00:33:38.50 Exhaust.open @00:33:39.93 Bypass.close @00:33:41.52 Pressure.in :default @00:33:41.58 Pressure.hold @00:33:41.80 SamplePump.in :prime @00:33:41.86 SamplePump.seek 100ml,5:16 @00:35:47.52 Pressure.stop @00:35:47.58 Bypass.open @00:35:48.35 Intake.close @00:35:49.20 Toroid.in :hold @00:35:49.26 Toroid.seek :crack @00:35:51.15 Toroid.seek :cartridge @00:35:52.25 Toroid.in :default @00:35:52.32 Toroid.seek :clear @00:35:54.34 Pressurizing cartridge to 14psi above 12.8psia @00:35:54.36 SamplePump.setPosition! 0ml @00:35:54.40 Intake.open @00:35:55.17 Bypass.close @00:35:55.95 Exhaust.close @00:35:56.76 Pressure.in :default @00:35:56.82 Pressure.hold @00:35:56.99 SamplePump.in :charge @00:35:57.06 SamplePump.seek 2.3ml,41 seconds @00:36:06.46 Pressure.stop @00:36:06.51 Intake.close @00:36:06.88 Pressurized cartridge with 0.394ml to 14.7psi @00:36:06.89 Isolated @24.6psia (11.8psi) @00:36:06.91 Verifying cartridge holds at least 0.75..0.85 of 24.6psia @00:36:06.94 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @00:36:11.52 Cartridge pressure fell 1% to 24.4psia @00:36:11.60 Sampling 400.0ml @00:36:11.61 SamplePump.setPosition! 0ml @00:36:11.66 Exhaust.open @00:36:12.38 Intake.open @00:36:13.25 Pressure.in :default @00:36:13.31 Pressure.hold @00:36:13.54 SamplePump.in :sample @00:36:13.60 SamplePump.seek 400ml,27:11 @00:36:16.28 Cartridge 19 filtering 1/400ml at 0.34ml/s with 13.9psi vacuum @00:37:16.29 Cartridge 19 filtering 21/400ml at 0.34ml/s with 13.5psi vacuum @00:38:16.29 Cartridge 19 filtering 41/400ml at 0.33ml/s with 13.2psi vacuum @00:39:16.29 Cartridge 19 filtering 60/400ml at 0.32ml/s with 14.7psi vacuum @00:40:16.29 Cartridge 19 filtering 79/400ml at 0.30ml/s with 13.9psi vacuum @00:41:16.29 Cartridge 19 filtering 97/400ml at 0.28ml/s with 12.3psi vacuum @00:42:16.29 Cartridge 19 filtering 115/400ml at 0.27ml/s with 12.1psi vacuum @00:43:16.29 Cartridge 19 filtering 131/400ml at 0.30ml/s with 14.0psi vacuum @00:44:16.29 Cartridge 19 filtering 148/400ml at 0.25ml/s with 14.3psi vacuum @00:45:16.29 Cartridge 19 filtering 164/400ml at 0.25ml/s with 13.3psi vacuum @00:46:16.29 Cartridge 19 filtering 180/400ml at 0.27ml/s with 13.7psi vacuum @00:47:16.29 Cartridge 19 filtering 196/400ml at 0.25ml/s with 17.7psi vacuum @00:48:08.11 -> Cmd.stop @00:48:08.12 Pressure.stop @00:48:08.23 Intake.close @00:48:08.32 Exhaust.close @00:48:08.45 Toroid.in :hold @00:48:08.50 Toroid.seek :bypass @00:48:10.11 Toroid.in :default @00:48:10.16 Toroid.seek :clear @00:48:11.91 Gate.power :heat,:OFF @00:48:11.92 Gate.power :cartridge,:OFF @00:48:12.02 Carousel.seek 19 @00:48:12.20 Plunge.seek :locked @00:48:12.44 Air.in :unlocking @00:48:12.56 Air.seek :clear @00:48:12.64 Lysis1.in :unlocking @00:48:12.75 Lysis1.seek :clear @00:48:14.18 Gate.power :motor,:OFF @00:48:14.20 Gate.power :core,:OFF @00:48:14.22 Gate.power :analytic1,:OFF @00:48:14.24 Gate.power :analytic2,:OFF @00:48:14.27 Gate.power :analytic3,:OFF @00:48:14.29 Gate.power :main,:OFF @00:48:14.31 Safely stopped and ready to power off ##-- file closed 2021-08-12T05:49:58.808Z ##-- file opened 2021-08-12T05:49:58.810Z @01:49:59.13 -> showStatus @01:50:01.18 -> Cmd.loadCartridge -4 @01:50:01.23 Gate.power :main,:ON,limit to 5000mA @01:50:01.25 Gate.power :analytic1,:ON,limit to 5000mA @01:50:01.28 Gate.power :analytic2,:ON,limit to 5000mA @01:50:01.30 Gate.power :analytic3,:ON,limit to 5000mA @01:50:01.43 Powering core bus @01:50:03.72 SPR Module 1, FW Rev. B, FPGA Rev. C @01:50:04.18 Archive.configure :default @01:50:04.31 Air.configure :default @01:50:04.41 Lysis1.configure :default @01:50:04.52 Lysis2.configure :default @01:50:04.64 Plunge.configure :default @01:50:04.77 SamplePump.configure :default @01:50:04.91 ExtraPump.configure :default @01:50:05.03 Toroid.configure :default @01:50:05.15 Carousel.configure :default @01:50:05.27 Pressure.configure :default @01:50:05.39 PinPort.configure :default @01:50:05.45 All elf controllers running firmware version 4.741 @01:50:05.48 Gate.power :motor,:ON,limit to 5000mA @01:50:05.63 Archive.in :default @01:50:05.94 Archive.home.jog 0 @01:50:06.10 Toroid.in :default @01:50:06.18 Plunge.in :default @01:50:06.21 Lysis1.in :default @01:50:06.27 PinPort.in :default @01:50:06.32 Lysis2.in :default @01:50:06.35 Air.in :default @01:50:06.53 Toroid.home.jog 0 @01:50:06.56 Lysis1.home.jog 0 @01:50:06.60 Plunge.home.jog 0 @01:50:06.63 Lysis2.home.jog 0 @01:50:06.68 PinPort.home.jog 0 @01:50:06.76 Air.home.jog 0 @01:50:07.19 Archive.in :homing @01:50:07.40 Toroid.in :homing @01:50:07.42 Plunge.in :homing @01:50:07.45 Archive.jog 1396 @01:50:07.50 PinPort.in :homing @01:50:07.53 Lysis1.in :homing @01:50:07.56 Air.in :homing @01:50:07.60 Lysis2.in :homing @01:50:07.77 Plunge.jog 1548 @01:50:07.84 Toroid.jog 623 @01:50:07.89 PinPort.jog -108 @01:50:07.91 Lysis1.jog 1411 @01:50:07.93 Air.jog 1415 @01:50:07.99 Lysis2.jog 1410 @01:50:08.29 PinPort.setRawPosition! 0 @01:50:08.33 PinPort.seek :home @01:50:08.42 PinPort.in :default @01:50:08.83 Archive.setRawPosition! 50 @01:50:08.88 Archive.seek :home @01:50:09.17 Archive.in :default @01:50:09.27 Plunge.setRawPosition! 50 @01:50:09.31 Plunge.seek :home @01:50:09.39 Lysis2.setRawPosition! 50 @01:50:09.42 Lysis1.setRawPosition! 50 @01:50:09.44 Air.setRawPosition! 50 @01:50:09.52 Lysis2.seek :home @01:50:09.62 Lysis1.seek :home @01:50:09.67 Air.seek :home @01:50:09.80 Plunge.in :default @01:50:09.90 Lysis2.in :default @01:50:10.01 Air.in :default @01:50:10.03 Lysis1.in :default @01:50:10.18 Toroid.setRawPosition! 0 @01:50:10.23 Toroid.seek :home @01:50:10.32 Toroid.in :default @01:50:10.48 Toroid.seek :clear @01:50:12.09 Carousel.in :default @01:50:12.15 Carousel.home.jog 0 @01:50:12.65 Carousel.in :default @01:50:12.72 Carousel.seek 19 @01:50:12.89 Selecting Cartridge 22 @01:50:13.13 Carousel.seek 22 @01:50:14.02 Lysis1.in :fast @01:50:14.09 Lysis1.seek :locked @01:50:16.68 Plunge.seek :locked @01:50:16.81 Air.in :fast @01:50:16.87 Air.seek :locked @01:50:19.47 Powering cartridge bus @01:50:22.09 Gate.setIR 1750,5000,0,6 @01:50:22.22 Gate.setIR 0,5000,0,6 @01:50:22.27 Gate.power :cartridge,:OFF @01:50:22.80 Mounted archive Cartridge @01:50:40.75 -> Cmd.startFiltering @01:50:41.20 Priming sample loop w/100ml, bypass w/10ml @01:50:41.22 SamplePump.setPosition! 0ml @01:50:41.26 Bypass.open @01:50:42.76 Intake.open @01:50:43.58 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @01:50:46.18 Vacuum tare is 51% of full scale @01:50:46.30 Pressure.in :default @01:50:46.36 Pressure.hold @01:50:46.53 SamplePump.in :prime @01:50:46.60 SamplePump.seek 10ml,59 seconds @01:50:55.86 Pressure.stop @01:50:55.91 SamplePump.setPosition! 0ml @01:50:55.96 Exhaust.open @01:50:57.41 Bypass.close @01:50:58.97 Pressure.in :default @01:50:59.03 Pressure.hold @01:50:59.25 SamplePump.in :prime @01:50:59.32 SamplePump.seek 100ml,5:16 @01:53:04.87 Pressure.stop @01:53:04.93 Bypass.open @01:53:05.68 Intake.close @01:53:06.56 Toroid.in :hold @01:53:06.62 Toroid.seek :crack @01:53:08.50 Toroid.seek :cartridge @01:53:09.60 Toroid.in :default @01:53:09.67 Toroid.seek :clear @01:53:11.62 Pressurizing cartridge to 26psi above 13.3psia @01:53:11.63 SamplePump.setPosition! 0ml @01:53:11.68 Intake.open @01:53:12.45 Bypass.close @01:53:13.22 Exhaust.close @01:53:14.05 Pressure.in :default @01:53:14.11 Pressure.hold @01:53:14.28 SamplePump.in :charge @01:53:14.36 SamplePump.seek 2.3ml,42 seconds @01:53:21.81 Pressure.stop @01:53:21.87 Intake.close @01:53:22.70 Pressurized cartridge with 0.246ml to 26.5psi @01:53:22.72 Isolated @35.0psia (21.7psi) @01:53:22.74 Verifying cartridge holds at least 0.75..0.85 of 35.0psia @01:53:22.77 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @01:53:27.34 Cartridge pressure fell 2% to 34.3psia @01:53:27.42 Sampling 1000.0ml @01:53:27.43 SamplePump.setPosition! 0ml @01:53:27.48 Exhaust.open @01:53:28.19 Intake.open @01:53:29.04 Pressure.in :default @01:53:29.10 Pressure.hold @01:53:29.32 SamplePump.in :sample @01:53:29.39 SamplePump.seek 1000ml,1:23:53 @01:53:32.08 Cartridge 22 filtering 1/1000ml at 0.38ml/s with 20.6psi vacuum @01:54:32.08 Cartridge 22 filtering 23/1000ml at 0.34ml/s with 21.6psi vacuum @01:55:32.08 Cartridge 22 filtering 44/1000ml at 0.38ml/s with 21.7psi vacuum @01:56:32.08 Cartridge 22 filtering 64/1000ml at 0.30ml/s with 22.2psi vacuum @01:57:32.08 Cartridge 22 filtering 83/1000ml at 0.36ml/s with 23.7psi vacuum @01:58:32.09 Cartridge 22 filtering 102/1000ml at 0.34ml/s with 21.6psi vacuum @01:59:32.08 Cartridge 22 filtering 120/1000ml at 0.27ml/s with 28.6psi vacuum @02:00:32.08 Cartridge 22 filtering 137/1000ml at 0.26ml/s with 29.1psi vacuum @02:01:32.08 Cartridge 22 filtering 153/1000ml at 0.29ml/s with 21.9psi vacuum @02:02:32.08 Cartridge 22 filtering 168/1000ml at 0.22ml/s with 24.3psi vacuum @02:03:32.08 Cartridge 22 filtering 183/1000ml at 0.24ml/s with 22.1psi vacuum @02:04:32.08 Cartridge 22 filtering 198/1000ml at 0.25ml/s with 23.3psi vacuum @02:05:32.08 Cartridge 22 filtering 211/1000ml at 0.23ml/s with 23.7psi vacuum @02:06:32.08 Cartridge 22 filtering 225/1000ml at 0.22ml/s with 22.1psi vacuum @02:07:32.08 Cartridge 22 filtering 237/1000ml at 0.20ml/s with 24.0psi vacuum @02:08:32.08 Cartridge 22 filtering 250/1000ml at 0.21ml/s with 25.1psi vacuum @02:09:05.63 Flow averaged 0.27ml/s over 15:37 @02:09:05.66 Waiting up to 16 seconds for vacuum to reduce from 29.1 to 18.0psi @02:09:06.48 Relieved excess vacuum in .9 seconds @02:09:06.51 SamplePump.seek 1000ml,1:02:31 @02:09:29.22 Flow averaged 0.20ml/s over 23 seconds @02:09:29.25 Waiting up to 16 seconds for vacuum to reduce from 29.0 to 18.0psi @02:09:29.83 Relieved excess vacuum in .6 seconds @02:09:29.85 SamplePump.seek 1000ml,1:02:08 @02:09:32.07 Cartridge 22 filtering 261/1000ml at 0.20ml/s with 26.3psi vacuum @02:09:33.09 Flow estimate reduced to 0.16ml/s @02:09:33.12 Intake.close @02:09:33.18 Exhaust.close @02:09:33.36 Toroid.in :hold @02:09:33.42 Toroid.seek :bypass @02:09:35.05 Toroid.in :default @02:09:35.11 Toroid.seek :clear @02:09:36.73 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @02:09:36.74 Pressure.stop @02:09:36.93 Sampled 261.5ml @02:09:38.92 -> Cmd.startProcessing @02:09:39.29 Plunge.seek :reagent @02:09:40.13 Sample loop closed. Ready to Deliver Reagents @02:09:40.15 Archive.seek :sealed @02:09:44.41 Archive.seek :evac @02:09:58.35 delay 1:00 @02:10:58.38 Archive.seek :clear @02:11:16.09 RNAlater soak for 10:00 @02:21:16.11 Archive.seek :tVent @02:21:18.69 delay 1 second @02:21:19.71 Archive.seek :evac @02:21:35.33 delay 10 seconds @02:21:45.36 Archive.seek :tVent @02:22:00.98 delay 1 second @02:22:02.00 Archive.seek :evac @02:22:17.61 delay 10 seconds @02:22:27.64 Sample preserved and evacuated @02:22:28.57 -> Cmd.stop @02:22:28.71 Carousel.seek 22 @02:22:28.88 Plunge.seek :locked @02:22:29.83 Archive.in :unlocking @02:22:29.96 Archive.seek :clear @02:22:30.00 Air.in :unlocking @02:22:30.14 Air.seek :clear @02:22:30.22 Lysis1.in :unlocking @02:22:30.34 Lysis1.seek :clear @02:22:34.17 Gate.power :motor,:OFF @02:22:34.19 Gate.power :core,:OFF @02:22:34.21 Gate.power :analytic1,:OFF @02:22:34.23 Gate.power :analytic2,:OFF @02:22:34.25 Gate.power :analytic3,:OFF @02:22:34.27 Gate.power :main,:OFF @02:22:34.29 Safely stopped and ready to power off ##-- file closed 2021-08-12T06:31:33.173Z ##-- file opened 2021-08-12T06:31:33.177Z @02:31:33.94 -> showStatus @02:31:35.96 -> Cmd.loadCartridge -18 @02:31:36.01 Gate.power :main,:ON,limit to 5000mA @02:31:36.04 Gate.power :analytic1,:ON,limit to 5000mA @02:31:36.06 Gate.power :analytic2,:ON,limit to 5000mA @02:31:36.08 Gate.power :analytic3,:ON,limit to 5000mA @02:31:36.21 Powering core bus @02:31:38.50 SPR Module 1, FW Rev. B, FPGA Rev. C @02:31:38.95 Archive.configure :default @02:31:39.08 Air.configure :default @02:31:39.18 Lysis1.configure :default @02:31:39.29 Lysis2.configure :default @02:31:39.41 Plunge.configure :default @02:31:39.53 SamplePump.configure :default @02:31:39.68 ExtraPump.configure :default @02:31:39.80 Toroid.configure :default @02:31:39.92 Carousel.configure :default @02:31:40.04 Pressure.configure :default @02:31:40.15 PinPort.configure :default @02:31:40.22 All elf controllers running firmware version 4.741 @02:31:40.25 Gate.power :motor,:ON,limit to 5000mA @02:31:40.38 Archive.in :default @02:31:40.64 Archive.home.jog 0 @02:31:40.86 Toroid.in :default @02:31:40.94 Plunge.in :default @02:31:40.97 Lysis1.in :default @02:31:41.02 PinPort.in :default @02:31:41.08 Lysis2.in :default @02:31:41.11 Air.in :default @02:31:41.31 Toroid.home.jog 0 @02:31:41.33 Plunge.home.jog 0 @02:31:41.36 Lysis1.home.jog 0 @02:31:41.38 PinPort.home.jog 0 @02:31:41.41 Air.home.jog 0 @02:31:41.44 Lysis2.home.jog 0 @02:31:41.80 Archive.in :homing @02:31:42.01 Toroid.in :homing @02:31:42.04 Plunge.in :homing @02:31:42.09 Archive.jog 161 @02:31:42.15 Lysis1.in :homing @02:31:42.17 PinPort.in :homing @02:31:42.39 Toroid.jog 629 @02:31:42.42 Lysis1.jog 1412 @02:31:42.46 Plunge.jog 1563 @02:31:42.49 PinPort.jog -108 @02:31:42.70 Air.in :homing @02:31:42.74 Lysis2.in :homing @02:31:42.82 Air.jog 1411 @02:31:42.85 Lysis2.jog 1411 @02:31:42.96 PinPort.setRawPosition! 0 @02:31:42.98 Archive.setRawPosition! 50 @02:31:43.05 PinPort.seek :home @02:31:43.09 Archive.seek :home @02:31:43.21 PinPort.in :default @02:31:43.41 Archive.in :default @02:31:43.70 Lysis1.setRawPosition! 50 @02:31:43.74 Lysis1.seek :home @02:31:43.83 Plunge.setRawPosition! 50 @02:31:43.87 Plunge.seek :home @02:31:44.05 Lysis2.setRawPosition! 50 @02:31:44.07 Lysis1.in :default @02:31:44.09 Air.setRawPosition! 50 @02:31:44.20 Lysis2.seek :home @02:31:44.29 Air.seek :home @02:31:44.41 Plunge.in :default @02:31:44.54 Lysis2.in :default @02:31:44.64 Toroid.setRawPosition! 0 @02:31:44.66 Air.in :default @02:31:44.73 Toroid.seek :home @02:31:44.85 Toroid.in :default @02:31:45.11 Toroid.seek :clear @02:31:46.72 Carousel.in :default @02:31:46.78 Carousel.home.jog 0 @02:31:47.28 Carousel.in :default @02:31:47.34 Carousel.seek 22 @02:31:47.48 Selecting Cartridge 17 @02:31:47.73 Carousel.seek 17 @02:31:49.02 Lysis1.in :fast @02:31:49.08 Lysis1.seek :locked @02:31:51.67 Plunge.seek :locked @02:31:51.79 Air.in :fast @02:31:51.85 Air.seek :locked @02:31:54.45 Powering cartridge bus @02:31:57.07 Gate.setIR 1750,5000,0,6 @02:31:57.20 Gate.setIR 0,5000,0,6 @02:31:57.26 PuckHeater.configure :default @02:31:57.31 Gate.power :heat,:ON,limit to 5000mA @02:31:58.02 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @02:31:58.06 Using default puck heat loss curve for HeatingAccelInCarousel @02:32:16.36 -> Cmd.startFiltering @02:32:16.82 Priming sample loop w/100ml, bypass w/10ml @02:32:16.83 SamplePump.setPosition! 0ml @02:32:16.88 Bypass.open @02:32:18.39 Intake.open @02:32:19.23 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @02:32:21.79 Vacuum tare is 51% of full scale @02:32:21.91 Pressure.in :default @02:32:21.97 Pressure.hold @02:32:22.15 SamplePump.in :prime @02:32:22.21 SamplePump.seek 10ml,59 seconds @02:32:31.47 Pressure.stop @02:32:31.52 SamplePump.setPosition! 0ml @02:32:31.57 Exhaust.open @02:32:33.01 Bypass.close @02:32:34.58 Pressure.in :default @02:32:34.64 Pressure.hold @02:32:34.86 SamplePump.in :prime @02:32:34.93 SamplePump.seek 100ml,5:16 @02:34:40.37 Pressure.stop @02:34:40.43 Bypass.open @02:34:41.17 Intake.close @02:34:42.43 Toroid.in :hold @02:34:42.49 Toroid.seek :crack @02:34:44.01 Toroid.seek :cartridge @02:34:45.09 Toroid.in :default @02:34:45.15 Toroid.seek :clear @02:34:47.11 Pressurizing cartridge to 14psi above 12.4psia @02:34:47.12 SamplePump.setPosition! 0ml @02:34:47.17 Intake.open @02:34:47.92 Bypass.close @02:34:48.68 Exhaust.close @02:34:49.48 Pressure.in :default @02:34:49.54 Pressure.hold @02:34:49.70 SamplePump.in :charge @02:34:49.77 SamplePump.seek 2.3ml,41 seconds @02:34:59.50 Pressure.stop @02:34:59.55 Intake.close @02:35:00.39 Pressurized cartridge with 0.421ml to 14.0psi @02:35:00.40 Isolated @23.8psia (11.4psi) @02:35:00.42 Verifying cartridge holds at least 0.75..0.85 of 23.8psia @02:35:00.45 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @02:35:05.02 Cartridge pressure fell 1% to 23.6psia @02:35:05.10 Sampling 75.0ml @02:35:05.11 SamplePump.setPosition! 0ml @02:35:05.16 Exhaust.open @02:35:05.84 Intake.open @02:35:06.23 Pressure.in :default @02:35:06.29 Pressure.hold @02:35:06.51 SamplePump.in :sample @02:35:06.58 SamplePump.seek 75ml,5:31 @02:35:09.26 Cartridge 17 filtering 1/75ml at 0.34ml/s with 13.4psi vacuum @02:36:09.26 Cartridge 17 filtering 21/75ml at 0.32ml/s with 13.8psi vacuum @02:37:09.27 Cartridge 17 filtering 41/75ml at 0.32ml/s with 14.0psi vacuum @02:38:09.26 Cartridge 17 filtering 61/75ml at 0.31ml/s with 13.3psi vacuum @02:38:53.18 Pressure.stop @02:38:53.30 Intake.close @02:38:53.34 Exhaust.close @02:38:53.49 Toroid.in :hold @02:38:53.56 Toroid.seek :bypass @02:38:55.18 Toroid.in :default @02:38:55.24 Toroid.seek :clear @02:38:56.86 Sampled 75.0ml @02:38:58.34 -> Cmd.startProcessing @02:38:59.86 SPR.command \"read_sequences\" @02:39:00.09 SPR.command \"stats_on\" @02:39:00.13 SPR.command \"start 0 1\" @02:39:00.94 SPR.start /var/log/esp/spr21aug1202h.spr.bz2 @02:39:03.44 Handoff.select :waste @02:39:10.14 Syringe tare is 20% of full scale @02:39:10.16 PinPort.in :locking @02:39:10.24 PinPort.seek :locked @02:39:10.71 Archive.in :fast @02:39:10.75 Lysis2.in :fast @02:39:10.82 Lysis1.seek 1350uL @02:39:10.89 Archive.seek :full @02:39:10.94 Lysis2.seek :full @02:39:13.07 Plunge.seek :reagent @02:39:13.98 Evacuating puck with filter bubble point of 24psi @02:39:14.00 PinPort.seek :locked @02:39:14.09 Handoff.select :waste @02:39:20.68 Air syringe pumping up to 1 stroke to reach 8psi @02:39:20.69 Air stroke #1 of 1 @02:39:20.77 Air.in :pressurize @02:39:20.84 Air.seek :pressurized @02:39:32.09 2.0psi after pressurize @02:39:32.10 Air.in :compress @02:39:32.18 Air.seek :evac @02:39:36.27 1.4psi after compress @02:39:48.86 0.5psi after compress (stable) @02:39:48.89 Air.in :default @02:39:48.96 Air.hold @02:39:49.13 at 0.6psi Opened to waste @02:39:49.14 Handoff.select :blocked @02:39:49.28 Archive syringe pumping up to 8 strokes to reach 24psi @02:39:49.29 Archive stroke #1 of 8 @02:39:49.37 Archive.in :pressurize @02:39:49.43 Archive.seek :pressurized @02:40:02.17 3.0psi after pressurize @02:40:02.18 Archive.in :compress @02:40:02.26 Archive.seek :empty @02:40:18.60 13.0psi after compress @02:40:31.18 13.9psi after compress (stable) @02:40:31.20 Archive.in :gulp @02:40:31.27 Archive.seek :topVent @02:40:38.47 14.0psi after gulp @02:40:51.04 14.0psi after gulp (stable) @02:40:51.05 Archive stroke #2 of 8 @02:40:51.14 Archive.in :pressurize @02:40:51.20 Archive.seek :pressurized @02:41:03.95 14.0psi after pressurize @02:41:03.96 Archive.in :compress @02:41:04.03 Archive.seek :empty @02:41:19.19 Reached 24.1 of 24.0psi after pumping 3445uL (1.99 strokes) @02:41:19.20 Archive.in :default @02:41:19.27 Archive.hold @02:41:19.34 Handoff.select :waste @02:41:30.52 delay 1:00 @02:42:30.54 Handoff.select :blocked @02:42:53.26 Handoff.select :waste @02:42:53.39 0.7psi at evacEnd @02:42:53.40 Handoff.select :blocked @02:42:53.53 Archive.in :fast @02:42:53.60 Archive.seek :topVent @02:43:07.26 Stable @0.7psi after Handoff blocked @02:43:07.28 Archive syringe pumping up to 8 strokes to reach 16psi @02:43:07.30 Archive stroke #1 of 8 @02:43:07.39 Archive.in :pressurize @02:43:07.47 Archive.seek :pressurized @02:43:20.20 0.8psi after pressurize @02:43:20.62 Archive.in :compress @02:43:20.72 Archive.seek :empty @02:43:36.60 11.9psi after compress @02:43:49.17 13.2psi after compress (stable) @02:43:49.19 Archive.in :gulp @02:43:49.26 Archive.seek :topVent @02:43:56.45 13.2psi after gulp @02:44:09.02 13.2psi after gulp (stable) @02:44:09.04 Archive stroke #2 of 8 @02:44:09.13 Archive.in :pressurize @02:44:09.19 Archive.seek :pressurized @02:44:21.92 13.2psi after pressurize @02:44:21.93 Archive.in :compress @02:44:22.01 Archive.seek :empty @02:44:29.13 Reached 15.7 of 15.7psi after pumping 3273uL (1.89 strokes) @02:44:29.14 Archive.in :default @02:44:29.23 Archive.hold @02:44:29.34 Handoff.select :waste @02:44:42.04 delay 1:00 @02:45:42.06 Handoff.select :blocked @02:46:04.73 Handoff.select :waste @02:46:04.85 0.5psi at evacEnd @02:46:04.87 Air.seek :fullVent @02:46:06.31 Archive.in :fast @02:46:06.37 Archive.seek :topVent @02:46:12.85 Seawater evacuated, Presure above/below filter released. Lysis begins @02:46:12.96 Lysis1.in :slow @02:46:13.02 Lysis1.seek 1050uL @02:46:43.03 delay 5 seconds @02:46:48.05 Handoff.select :blocked @02:46:48.23 Lysis1.seek 450uL @02:47:48.18 delay 3 seconds @02:47:51.27 Air.in to mix into 1.37806 parts Lysis1 @02:47:51.70 Air.seek :evac @02:47:51.73 Lysis1.seek 300uL @02:48:06.84 Lysis1.seek :empty @02:48:36.93 Lysis buffer delivered @7.5psi @02:48:36.94 Polling Can every 2:00 @02:48:37.49 PuckHeater.configure :default #ambient 25.1C @02:48:37.54 PuckHeater.seek 79.6C @02:49:12.48 PuckHeater.hold 2:00,79.6C @02:51:12.29 Polling Can every 2:00 @02:51:12.83 PuckHeater.configure :default #ambient 25.1C @02:51:12.87 PuckHeater.hold 30 seconds,78.6C @02:51:43.00 Polling Can every 2:00 @02:51:43.54 PuckHeater.configure :default #ambient 25.1C @02:51:43.58 PuckHeater.hold 30 seconds,78.1C @02:52:13.70 Polling Can every 2:00 @02:52:14.24 PuckHeater.configure :default #ambient 25.1C @02:52:14.29 PuckHeater.hold 10:00,77.5C @03:02:12.79 Lysis complete. Syringe pressure @7.8psi @03:02:12.80 Handoff.select :blocked @03:02:12.85 Archive syringe pumping up to 5 strokes to reach 40psi @03:02:12.86 Archive stroke #1 of 5 @03:02:12.97 Archive.in :pressurize @03:02:13.03 Archive.seek :pressurized @03:02:25.77 8.4psi after pressurize @03:02:25.78 Archive.in :compress @03:02:25.86 Archive.seek :empty @03:02:41.75 40.0psi after compress @03:02:41.90 Reached 40.2 of 40.0psi after pumping 1730uL (1.00 strokes) @03:02:41.92 Archive.in :default @03:02:41.99 Archive.hold @03:02:42.05 delay 2 seconds @03:02:44.09 Archive.in :fast @03:02:44.17 Archive.seek :full @03:02:51.38 Lysis1.seek 138uL @03:03:05.26 delay 1 second @03:03:06.29 Archive.seek :full @03:03:06.31 Lysis1.seek :empty @03:03:20.29 Initial air pressure @39.4psi @03:03:20.36 Lysis1.in :extract @03:03:20.43 Lysis1.seek 600uL,nil @03:03:31.16 Initial 600uL lysate @17.1psi @03:03:31.17 Handoff.select :blocked @03:03:31.26 Initial air pressure @17.1psi @03:03:31.34 Lysis1.seek 825uL,nil @03:03:33.10 Archive syringe pumping up to 6 strokes to reach 17psi @03:03:33.11 Archive stroke #1 of 6 @03:03:33.20 Archive.in :pressurize @03:03:33.27 Archive.seek :pressurized @03:03:46.04 15.0psi after pressurize @03:03:46.05 Archive.in :compress @03:03:46.12 Archive.seek :empty @03:03:51.92 Archive.in :default @03:03:51.97 Archive.hold @03:03:52.25 Recovered 825uL lysate @16.2psi @03:03:52.27 Air.in :halfSpeed @03:03:52.34 Air.seek :fullVent @03:03:54.58 Puck Top Vented, bottom @13.6psi @03:03:54.59 Handoff.select :waste @03:03:54.71 delay 5 seconds @03:03:59.79 Handoff open to waste, bottom @0.5psi @03:03:59.81 Air.in :default @03:03:59.89 Air.seek :evac @03:04:01.33 Lysis2.in :DAmix @03:04:01.43 Lysis1.in :DAmix @03:04:01.95 Lysis2.seek 1480uL @03:04:01.99 Lysis1.seek 575uL @03:04:37.06 delay 5 seconds @03:04:42.08 Handoff.select SPR @03:04:42.21 SPR.command \"start 2 14\" @03:04:42.79 SPR stabilized @03:04:42.92 delay 10 seconds @03:04:52.94 SPR.command \"start 2 0\" @03:04:56.15 Waiting up to 11:00 for SPR to enter state 1 @03:06:53.82 SPR.command \"hold_on\" @03:06:55.21 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @03:06:55.63 Lysis2.seek 1180uL @03:06:55.70 Lysis1.seek 275uL @03:07:37.73 delay 15 seconds @03:07:52.75 SPR.command \"hold_off\" @03:07:55.86 Waiting up to 38:00 for SPR to enter state 7 @03:31:53.01 SPR analysis completed @03:31:57.24 Ended log spr21aug1202h.spr.bz2 @03:31:57.25 SPR.command \"stats_off\" @03:31:58.87 -> Cmd.stop @03:31:58.88 Archive.stop @03:31:59.00 Handoff.select :close @03:31:59.13 Gate.power :heat,:OFF @03:31:59.15 Gate.power :cartridge,:OFF @03:31:59.24 Carousel.seek 17 @03:31:59.42 Plunge.seek :locked @03:32:00.31 PinPort.in :unlocking @03:32:00.37 PinPort.seek :clear @03:32:00.92 Archive.in :unlocking @03:32:01.04 Archive.seek :clear @03:32:01.11 Air.in :unlocking @03:32:01.23 Air.seek :clear @03:32:01.30 Lysis1.in :unlocking @03:32:01.42 Lysis1.seek :clear @03:32:01.49 Lysis2.in :unlocking @03:32:01.56 Lysis2.seek :clear @03:32:05.13 Gate.power :motor,:OFF @03:32:05.15 Gate.power :core,:OFF @03:32:05.17 Gate.power :analytic1,:OFF @03:32:05.19 Gate.power :analytic2,:OFF @03:32:05.21 Gate.power :analytic3,:OFF @03:32:05.23 Gate.power :main,:OFF @03:32:05.25 Safely stopped and ready to power off ##-- file closed 2021-08-12T14:06:59.647Z ##-- file opened 2021-08-12T14:06:59.651Z @10:06:59.71 -> showStatus @10:07:01.74 -> Cmd.loadCartridge -4 @10:07:01.80 Gate.power :main,:ON,limit to 5000mA @10:07:01.81 Gate.power :analytic1,:ON,limit to 5000mA @10:07:01.84 Gate.power :analytic2,:ON,limit to 5000mA @10:07:01.86 Gate.power :analytic3,:ON,limit to 5000mA @10:07:01.99 Powering core bus @10:07:04.28 SPR Module 1, FW Rev. B, FPGA Rev. C @10:07:04.74 Archive.configure :default @10:07:04.87 Air.configure :default @10:07:04.97 Lysis1.configure :default @10:07:05.09 Lysis2.configure :default @10:07:05.21 Plunge.configure :default @10:07:05.33 SamplePump.configure :default @10:07:05.47 ExtraPump.configure :default @10:07:05.58 Toroid.configure :default @10:07:05.70 Carousel.configure :default @10:07:05.83 Pressure.configure :default @10:07:05.94 PinPort.configure :default @10:07:06.00 All elf controllers running firmware version 4.741 @10:07:06.03 Gate.power :motor,:ON,limit to 5000mA @10:07:06.16 Archive.in :default @10:07:06.49 Archive.home.jog 0 @10:07:06.64 Toroid.in :default @10:07:06.72 Plunge.in :default @10:07:06.75 Lysis2.in :default @10:07:06.79 Air.in :default @10:07:06.82 PinPort.in :default @10:07:06.88 Lysis1.in :default @10:07:07.06 Plunge.home.jog 0 @10:07:07.11 Toroid.home.jog 0 @10:07:07.13 Lysis2.home.jog 0 @10:07:07.15 Air.home.jog 0 @10:07:07.18 PinPort.home.jog 0 @10:07:07.21 Lysis1.home.jog 0 @10:07:07.57 Archive.in :homing @10:07:07.88 Toroid.in :homing @10:07:07.90 Plunge.in :homing @10:07:07.93 Air.in :homing @10:07:07.97 Archive.jog 1390 @10:07:08.00 PinPort.in :homing @10:07:08.03 Lysis1.in :homing @10:07:08.07 Lysis2.in :homing @10:07:08.26 Plunge.jog 1544 @10:07:08.28 Toroid.jog 630 @10:07:08.33 Air.jog 1410 @10:07:08.37 PinPort.jog -108 @10:07:08.40 Lysis1.jog 1411 @10:07:08.51 Lysis2.jog 1408 @10:07:08.74 PinPort.setRawPosition! 0 @10:07:08.79 PinPort.seek :home @10:07:08.87 PinPort.in :default @10:07:09.30 Archive.setRawPosition! 50 @10:07:09.35 Archive.seek :home @10:07:09.65 Archive.in :default @10:07:09.67 Air.setRawPosition! 50 @10:07:09.80 Air.seek :home @10:07:09.89 Plunge.setRawPosition! 50 @10:07:09.91 Lysis2.setRawPosition! 50 @10:07:09.94 Lysis1.setRawPosition! 50 @10:07:10.03 Lysis2.seek :home @10:07:10.08 Plunge.seek :home @10:07:10.11 Lysis1.seek :home @10:07:10.30 Air.in :default @10:07:10.41 Lysis2.in :default @10:07:10.52 Plunge.in :default @10:07:10.54 Lysis1.in :default @10:07:10.68 Toroid.setRawPosition! 0 @10:07:10.73 Toroid.seek :home @10:07:10.83 Toroid.in :default @10:07:10.97 Toroid.seek :clear @10:07:12.58 Carousel.in :default @10:07:12.64 Carousel.home.jog 0 @10:07:13.13 Carousel.in :default @10:07:13.20 Carousel.seek 54 @10:07:13.37 Selecting Cartridge 20 @10:07:13.61 Carousel.seek 20 @10:07:20.82 Lysis1.in :fast @10:07:20.88 Lysis1.seek :locked @10:07:23.48 Plunge.seek :locked @10:07:23.61 Air.in :fast @10:07:23.67 Air.seek :locked @10:07:26.27 Powering cartridge bus @10:07:28.89 Gate.setIR 1750,5000,0,6 @10:07:29.02 Gate.setIR 0,5000,0,6 @10:07:29.07 Gate.power :cartridge,:OFF @10:07:29.60 Mounted archive Cartridge @10:07:41.34 -> Cmd.startFiltering @10:07:41.80 Priming sample loop w/100ml, bypass w/10ml @10:07:41.81 SamplePump.setPosition! 0ml @10:07:41.85 Bypass.open @10:07:43.38 Intake.open @10:07:44.20 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:07:46.77 Vacuum tare is 51% of full scale @10:07:46.93 Pressure.in :default @10:07:47.02 Pressure.hold @10:07:47.20 SamplePump.in :prime @10:07:47.26 SamplePump.seek 10ml,59 seconds @10:07:56.52 Pressure.stop @10:07:56.57 SamplePump.setPosition! 0ml @10:07:56.62 Exhaust.open @10:07:58.07 Bypass.close @10:07:59.67 Pressure.in :default @10:07:59.73 Pressure.hold @10:07:59.95 SamplePump.in :prime @10:08:00.02 SamplePump.seek 100ml,5:16 @10:10:05.43 Pressure.stop @10:10:05.49 Bypass.open @10:10:06.26 Intake.close @10:10:07.13 Toroid.in :hold @10:10:07.57 Toroid.seek :crack @10:10:09.08 Toroid.seek :cartridge @10:10:10.17 Toroid.in :default @10:10:10.23 Toroid.seek :clear @10:10:12.19 Pressurizing cartridge to 26psi above 12.4psia @10:10:12.20 SamplePump.setPosition! 0ml @10:10:12.25 Intake.open @10:10:12.98 Bypass.close @10:10:13.72 Exhaust.close @10:10:14.54 Pressure.in :default @10:10:14.60 Pressure.hold @10:10:14.77 SamplePump.in :charge @10:10:14.83 SamplePump.seek 2.3ml,42 seconds @10:10:22.73 Pressure.stop @10:10:22.78 Intake.close @10:10:23.63 Pressurized cartridge with 0.276ml to 27.9psi @10:10:23.64 Isolated @35.0psia (22.5psi) @10:10:23.65 Verifying cartridge holds at least 0.75..0.85 of 35.0psia @10:10:23.68 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:10:28.30 Sampling 1000.0ml @10:10:28.32 SamplePump.setPosition! 0ml @10:10:28.37 Exhaust.open @10:10:29.09 Intake.open @10:10:29.50 Pressure.in :default @10:10:29.56 Pressure.hold @10:10:29.78 SamplePump.in :sample @10:10:29.85 SamplePump.seek 1000ml,1:23:53 @10:10:32.53 Cartridge 20 filtering 1/1000ml at 0.41ml/s with 23.7psi vacuum @10:11:32.54 Cartridge 20 filtering 23/1000ml at 0.34ml/s with 22.0psi vacuum @10:12:32.54 Cartridge 20 filtering 44/1000ml at 0.32ml/s with 23.4psi vacuum @10:13:32.54 Cartridge 20 filtering 64/1000ml at 0.30ml/s with 24.1psi vacuum @10:14:32.54 Cartridge 20 filtering 83/1000ml at 0.30ml/s with 21.6psi vacuum @10:15:32.54 Cartridge 20 filtering 99/1000ml at 0.20ml/s with 24.4psi vacuum @10:16:32.54 Cartridge 20 filtering 112/1000ml at 0.26ml/s with 22.2psi vacuum @10:17:32.54 Cartridge 20 filtering 127/1000ml at 0.28ml/s with 22.7psi vacuum @10:18:32.54 Cartridge 20 filtering 143/1000ml at 0.22ml/s with 24.2psi vacuum @10:19:32.54 Cartridge 20 filtering 159/1000ml at 0.27ml/s with 23.4psi vacuum @10:20:32.54 Cartridge 20 filtering 173/1000ml at 0.27ml/s with 23.6psi vacuum @10:21:32.54 Cartridge 20 filtering 187/1000ml at 0.25ml/s with 22.9psi vacuum @10:22:32.54 Cartridge 20 filtering 200/1000ml at 0.22ml/s with 22.8psi vacuum @10:23:32.54 Cartridge 20 filtering 213/1000ml at 0.22ml/s with 22.7psi vacuum @10:24:32.54 Cartridge 20 filtering 226/1000ml at 0.20ml/s with 25.2psi vacuum @10:25:30.49 Flow averaged 0.26ml/s over 15:01 @10:25:30.52 Waiting up to 12 seconds for vacuum to reduce from 27.7 to 18.0psi @10:25:31.09 Relieved excess vacuum in .6 seconds @10:25:31.12 SamplePump.seek 1000ml,1:04:05 @10:25:32.53 Cartridge 20 filtering 238/1000ml at 0.20ml/s with 29.6psi vacuum @10:25:52.29 Flow averaged 0.20ml/s over 22 seconds @10:25:52.32 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi @10:25:52.90 Relieved excess vacuum in .6 seconds @10:25:52.92 SamplePump.seek 1000ml,1:03:44 @10:25:56.18 Flow estimate reduced to 0.16ml/s @10:25:56.21 Intake.close @10:25:56.26 Exhaust.close @10:25:56.45 Toroid.in :hold @10:25:56.51 Toroid.seek :bypass @10:25:58.14 Toroid.in :default @10:25:58.20 Toroid.seek :clear @10:25:59.82 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @10:25:59.83 Pressure.stop @10:26:00.01 Sampled 242.3ml @10:26:02.34 -> Cmd.startProcessing @10:26:02.72 Plunge.seek :reagent @10:26:03.56 Sample loop closed. Ready to Deliver Reagents @10:26:03.57 Archive.seek :sealed @10:26:07.84 Archive.seek :evac @10:26:21.78 delay 1:00 @10:27:21.80 Archive.seek :clear @10:27:39.51 RNAlater soak for 10:00 @10:37:39.53 Archive.seek :tVent @10:37:42.11 delay 1 second @10:37:43.13 Archive.seek :evac @10:37:58.74 delay 10 seconds @10:38:08.77 Archive.seek :tVent @10:38:24.38 delay 1 second @10:38:25.40 Archive.seek :evac @10:38:41.01 delay 10 seconds @10:38:51.04 Sample preserved and evacuated @10:38:51.93 -> Cmd.stop @10:38:52.07 Carousel.seek 20 @10:38:52.24 Plunge.seek :locked @10:38:53.20 Archive.in :unlocking @10:38:53.32 Archive.seek :clear @10:38:53.36 Air.in :unlocking @10:38:53.51 Lysis1.in :unlocking @10:38:53.55 Air.seek :clear @10:38:53.70 Lysis1.seek :clear @10:38:57.53 Gate.power :motor,:OFF @10:38:57.55 Gate.power :core,:OFF @10:38:57.57 Gate.power :analytic1,:OFF @10:38:57.59 Gate.power :analytic2,:OFF @10:38:57.62 Gate.power :analytic3,:OFF @10:38:57.64 Gate.power :main,:OFF @10:38:57.66 Safely stopped and ready to power off ##-- file closed 2021-08-12T14:47:56.001Z ##-- file opened 2021-08-12T14:47:56.003Z @10:47:56.40 -> showStatus @10:47:58.42 -> Cmd.loadCartridge -18 @10:47:58.48 Gate.power :main,:ON,limit to 5000mA @10:47:58.50 Gate.power :analytic1,:ON,limit to 5000mA @10:47:58.52 Gate.power :analytic2,:ON,limit to 5000mA @10:47:58.54 Gate.power :analytic3,:ON,limit to 5000mA @10:47:58.68 Powering core bus @10:48:00.96 SPR Module 1, FW Rev. B, FPGA Rev. C @10:48:01.41 Archive.configure :default @10:48:01.53 Air.configure :default @10:48:01.64 Lysis1.configure :default @10:48:01.74 Lysis2.configure :default @10:48:01.86 Plunge.configure :default @10:48:01.99 SamplePump.configure :default @10:48:02.14 ExtraPump.configure :default @10:48:02.25 Toroid.configure :default @10:48:02.37 Carousel.configure :default @10:48:02.49 Pressure.configure :default @10:48:02.61 PinPort.configure :default @10:48:02.67 All elf controllers running firmware version 4.741 @10:48:02.70 Gate.power :motor,:ON,limit to 5000mA @10:48:02.87 Archive.in :default @10:48:03.16 Archive.home.jog 0 @10:48:03.32 Toroid.in :default @10:48:03.37 Plunge.in :default @10:48:03.41 Lysis1.in :default @10:48:03.46 Air.in :default @10:48:03.51 Lysis2.in :default @10:48:03.55 PinPort.in :default @10:48:03.70 Toroid.home.jog 0 @10:48:03.74 Plunge.home.jog 0 @10:48:03.78 Lysis1.home.jog 0 @10:48:03.80 Air.home.jog 0 @10:48:03.84 PinPort.home.jog 0 @10:48:03.94 Lysis2.home.jog 0 @10:48:04.30 Archive.in :homing @10:48:04.52 Toroid.in :homing @10:48:04.56 Plunge.in :homing @10:48:04.59 Air.in :homing @10:48:04.62 Lysis1.in :homing @10:48:04.66 Archive.jog 1394 @10:48:04.69 PinPort.in :homing @10:48:04.72 Lysis2.in :homing @10:48:04.92 Toroid.jog 630 @10:48:04.97 Plunge.jog 1570 @10:48:05.01 Air.jog 1412 @10:48:05.04 PinPort.jog -108 @10:48:05.07 Lysis2.jog 1407 @10:48:05.11 Lysis1.jog 1411 @10:48:05.42 PinPort.setRawPosition! 0 @10:48:05.46 PinPort.seek :home @10:48:05.55 PinPort.in :default @10:48:06.00 Archive.setRawPosition! 50 @10:48:06.04 Archive.seek :home @10:48:06.34 Air.setRawPosition! 50 @10:48:06.40 Air.seek :home @10:48:06.50 Lysis2.setRawPosition! 50 @10:48:06.53 Archive.in :default @10:48:06.55 Plunge.setRawPosition! 50 @10:48:06.59 Lysis1.setRawPosition! 50 @10:48:06.71 Lysis2.seek :home @10:48:06.74 Plunge.seek :home @10:48:06.87 Lysis1.seek :home @10:48:06.98 Air.in :default @10:48:07.11 Lysis2.in :default @10:48:07.22 Toroid.setRawPosition! 0 @10:48:07.25 Plunge.in :default @10:48:07.27 Lysis1.in :default @10:48:07.38 Toroid.seek :home @10:48:07.53 Toroid.in :default @10:48:07.77 Toroid.seek :clear @10:48:09.38 Carousel.in :default @10:48:09.45 Carousel.home.jog 0 @10:48:09.93 Carousel.in :default @10:48:09.99 Carousel.seek 20 @10:48:10.13 Selecting Cartridge 15 @10:48:10.37 Carousel.seek 15 @10:48:11.69 Lysis1.in :fast @10:48:11.75 Lysis1.seek :locked @10:48:14.34 Plunge.seek :locked @10:48:14.46 Air.in :fast @10:48:14.53 Air.seek :locked @10:48:17.14 Powering cartridge bus @10:48:19.76 Gate.setIR 1750,5000,0,6 @10:48:19.89 Gate.setIR 0,5000,0,6 @10:48:19.95 PuckHeater.configure :default @10:48:20.00 Gate.power :heat,:ON,limit to 5000mA @10:48:20.73 Mounted lysis Cartridge board#Q05083 @10:48:20.77 Using default puck heat loss curve for HeatingAccelInCarousel @10:48:38.81 -> Cmd.startFiltering @10:48:39.27 Priming sample loop w/100ml, bypass w/10ml @10:48:39.28 SamplePump.setPosition! 0ml @10:48:39.33 Bypass.open @10:48:40.81 Intake.open @10:48:41.61 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:48:44.18 Vacuum tare is 51% of full scale @10:48:44.30 Pressure.in :default @10:48:44.36 Pressure.hold @10:48:44.53 SamplePump.in :prime @10:48:44.60 SamplePump.seek 10ml,59 seconds @10:48:53.86 Pressure.stop @10:48:53.91 SamplePump.setPosition! 0ml @10:48:53.96 Exhaust.open @10:48:55.40 Bypass.close @10:48:56.99 Pressure.in :default @10:48:57.06 Pressure.hold @10:48:57.27 SamplePump.in :prime @10:48:57.34 SamplePump.seek 100ml,5:16 @10:51:03.28 Pressure.stop @10:51:03.33 Bypass.open @10:51:04.10 Intake.close @10:51:04.96 Toroid.in :hold @10:51:05.02 Toroid.seek :crack @10:51:06.54 Toroid.seek :cartridge @10:51:07.62 Toroid.in :default @10:51:07.69 Toroid.seek :clear @10:51:09.64 Pressurizing cartridge to 14psi above 11.5psia @10:51:09.65 SamplePump.setPosition! 0ml @10:51:09.70 Intake.open @10:51:10.44 Bypass.close @10:51:11.21 Exhaust.close @10:51:12.05 Pressure.in :default @10:51:12.11 Pressure.hold @10:51:12.28 SamplePump.in :charge @10:51:12.35 SamplePump.seek 2.3ml,41 seconds @10:51:22.82 Pressure.stop @10:51:22.87 Intake.close @10:51:23.69 Pressurized cartridge with 0.489ml to 14.3psi @10:51:23.71 Isolated @23.2psia (11.7psi) @10:51:23.72 Verifying cartridge holds at least 0.75..0.85 of 23.2psia @10:51:23.76 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:51:28.38 Sampling 75.0ml @10:51:28.40 SamplePump.setPosition! 0ml @10:51:28.45 Exhaust.open @10:51:29.16 Intake.open @10:51:30.01 Pressure.in :default @10:51:30.07 Pressure.hold @10:51:30.29 SamplePump.in :sample @10:51:30.35 SamplePump.seek 75ml,5:31 @10:51:33.05 Cartridge 15 filtering 1/75ml at 0.32ml/s with 13.7psi vacuum @10:52:33.05 Cartridge 15 filtering 20/75ml at 0.33ml/s with 13.0psi vacuum @10:53:33.05 Cartridge 15 filtering 40/75ml at 0.31ml/s with 13.7psi vacuum @10:54:33.05 Cartridge 15 filtering 59/75ml at 0.30ml/s with 12.4psi vacuum @10:55:26.47 Pressure.stop @10:55:26.59 Intake.close @10:55:26.64 Exhaust.close @10:55:26.80 Toroid.in :hold @10:55:26.86 Toroid.seek :bypass @10:55:28.48 Toroid.in :default @10:55:28.54 Toroid.seek :clear @10:55:30.15 Sampled 75.0ml @10:55:31.72 -> Cmd.startProcessing @10:55:33.23 SPR.command \"read_sequences\" @10:55:33.89 SPR.command \"stats_on\" @10:55:33.93 SPR.command \"start 0 1\" @10:55:34.74 SPR.start /var/log/esp/spr21aug1210h.spr.bz2 @10:55:36.74 Handoff.select :waste @10:55:43.44 Syringe tare is 20% of full scale @10:55:43.46 PinPort.in :locking @10:55:43.53 PinPort.seek :locked @10:55:44.01 Archive.in :fast @10:55:44.07 Lysis2.in :fast @10:55:44.11 Lysis1.seek 1350uL @10:55:44.23 Archive.seek :full @10:55:44.28 Lysis2.seek :full @10:55:46.37 Plunge.seek :reagent @10:55:47.28 Evacuating puck with filter bubble point of 24psi @10:55:47.30 PinPort.seek :locked @10:55:47.38 Handoff.select :waste @10:55:54.47 Air syringe pumping up to 1 stroke to reach 8psi @10:55:54.48 Air stroke #1 of 1 @10:55:54.56 Air.in :pressurize @10:55:54.63 Air.seek :pressurized @10:56:05.87 0.7psi after pressurize @10:56:05.88 Air.in :compress @10:56:05.95 Air.seek :evac @10:56:10.03 3.2psi after compress @10:56:22.60 0.6psi after compress (stable) @10:56:22.63 Air.in :default @10:56:22.71 Air.hold @10:56:22.88 at 0.5psi Opened to waste @10:56:22.89 Handoff.select :blocked @10:56:23.03 Archive syringe pumping up to 8 strokes to reach 24psi @10:56:23.04 Archive stroke #1 of 8 @10:56:23.12 Archive.in :pressurize @10:56:23.18 Archive.seek :pressurized @10:56:35.94 2.4psi after pressurize @10:56:35.95 Archive.in :compress @10:56:36.02 Archive.seek :empty @10:56:52.34 14.1psi after compress @10:57:04.91 15.2psi after compress (stable) @10:57:04.93 Archive.in :gulp @10:57:05.00 Archive.seek :topVent @10:57:12.20 15.4psi after gulp @10:57:24.77 15.3psi after gulp (stable) @10:57:24.78 Archive stroke #2 of 8 @10:57:24.87 Archive.in :pressurize @10:57:24.93 Archive.seek :pressurized @10:57:37.66 15.3psi after pressurize @10:57:37.67 Archive.in :compress @10:57:37.74 Archive.seek :empty @10:57:50.48 Reached 24.1 of 24.0psi after pumping 3393uL (1.96 strokes) @10:57:50.49 Archive.in :default @10:57:50.56 Archive.hold @10:57:50.66 Handoff.select :waste @10:58:04.84 delay 1:00 @10:59:04.86 Handoff.select :blocked @10:59:27.55 Handoff.select :waste @10:59:27.66 0.6psi at evacEnd @10:59:27.67 Handoff.select :blocked @10:59:27.80 Archive.in :fast @10:59:27.87 Archive.seek :topVent @10:59:41.75 Stable @0.7psi after Handoff blocked @10:59:41.77 Archive syringe pumping up to 8 strokes to reach 16psi @10:59:41.79 Archive stroke #1 of 8 @10:59:41.88 Archive.in :pressurize @10:59:41.94 Archive.seek :pressurized @10:59:54.67 0.9psi after pressurize @10:59:54.69 Archive.in :compress @10:59:54.76 Archive.seek :empty @11:00:10.66 13.1psi after compress @11:00:23.23 14.0psi after compress (stable) @11:00:23.25 Archive.in :gulp @11:00:23.32 Archive.seek :topVent @11:00:30.53 14.1psi after gulp @11:00:43.10 14.0psi after gulp (stable) @11:00:43.11 Archive stroke #2 of 8 @11:00:43.20 Archive.in :pressurize @11:00:43.26 Archive.seek :pressurized @11:00:56.01 14.0psi after pressurize @11:00:56.03 Archive.in :compress @11:00:56.10 Archive.seek :empty @11:01:01.57 Reached 15.8 of 15.7psi after pumping 3237uL (1.87 strokes) @11:01:01.58 Archive.in :default @11:01:01.66 Archive.hold @11:01:01.74 Handoff.select :waste @11:01:27.41 delay 1:00 @11:02:27.43 Handoff.select :blocked @11:02:50.12 Handoff.select :waste @11:02:50.23 0.4psi at evacEnd @11:02:50.25 Air.seek :fullVent @11:02:51.69 Archive.in :fast @11:02:51.76 Archive.seek :topVent @11:02:58.11 Seawater evacuated, Presure above/below filter released. Lysis begins @11:02:58.20 Lysis1.in :slow @11:02:58.26 Lysis1.seek 1050uL @11:03:28.27 delay 5 seconds @11:03:33.31 Handoff.select :blocked @11:03:33.48 Lysis1.seek 450uL @11:04:33.42 delay 3 seconds @11:04:36.49 Air.in to mix into 1.37806 parts Lysis1 @11:04:36.94 Air.seek :evac @11:04:37.00 Lysis1.seek 300uL @11:04:52.08 Lysis1.seek :empty @11:05:22.17 Lysis buffer delivered @10.0psi @11:05:22.18 Polling Can every 2:00 @11:05:22.73 PuckHeater.configure :default #ambient 24.8C @11:05:22.80 PuckHeater.seek 79.6C @11:06:06.02 PuckHeater.hold 2:00,79.6C @11:08:05.95 Polling Can every 2:00 @11:08:06.49 PuckHeater.configure :default #ambient 24.9C @11:08:06.53 PuckHeater.hold 30 seconds,78.6C @11:08:36.69 Polling Can every 2:00 @11:08:37.23 PuckHeater.hold 30 seconds,78.1C @11:09:07.37 Polling Can every 2:00 @11:09:07.91 PuckHeater.hold 10:00,77.6C @11:19:07.06 Lysis complete. Syringe pressure @9.7psi @11:19:07.07 Handoff.select :blocked @11:19:07.12 Archive syringe pumping up to 5 strokes to reach 40psi @11:19:07.13 Archive stroke #1 of 5 @11:19:07.21 Archive.in :pressurize @11:19:07.28 Archive.seek :pressurized @11:19:20.00 10.1psi after pressurize @11:19:20.01 Archive.in :compress @11:19:20.08 Archive.seek :empty @11:19:32.97 Reached 40.3 of 40.0psi after pumping 1667uL (0.96 strokes) @11:19:32.98 Archive.in :default @11:19:33.06 Archive.hold @11:19:33.13 delay 2 seconds @11:19:35.15 Archive.in :fast @11:19:35.21 Archive.seek :full @11:19:42.18 Lysis1.seek 138uL @11:19:56.05 delay 1 second @11:19:57.08 Archive.seek :full @11:19:57.10 Lysis1.seek :empty @11:20:11.08 Initial air pressure @42.7psi @11:20:11.15 Lysis1.in :extract @11:20:11.24 Lysis1.seek 600uL,nil @11:20:20.26 Archive syringe pumping up to 6 strokes to reach 25psi @11:20:20.27 Archive stroke #1 of 6 @11:20:20.42 Archive.in :pressurize @11:20:20.48 Archive.seek :pressurized @11:20:33.20 15.7psi after pressurize @11:20:33.21 Archive.in :compress @11:20:33.28 Archive.seek :empty @11:20:35.23 Archive.in :default @11:20:35.28 Archive.hold @11:20:35.52 Initial 600uL lysate @16.5psi @11:20:35.53 Handoff.select :blocked @11:20:35.63 Initial air pressure @16.5psi @11:20:35.71 Lysis1.seek 825uL,nil @11:20:37.45 Archive syringe pumping up to 6 strokes to reach 17psi @11:20:37.46 Archive stroke #1 of 6 @11:20:37.59 Archive.in :compress @11:20:37.65 Archive.seek :empty @11:20:43.81 Archive.in :default @11:20:43.86 Archive.hold @11:20:44.12 Recovered 825uL lysate @16.3psi @11:20:44.14 Air.in :halfSpeed @11:20:44.21 Air.seek :fullVent @11:20:46.46 Puck Top Vented, bottom @17.7psi @11:20:46.47 Handoff.select :waste @11:20:46.60 delay 5 seconds @11:20:51.66 Handoff open to waste, bottom @0.2psi @11:20:51.68 Air.in :default @11:20:51.75 Air.seek :evac @11:20:53.18 Lysis2.in :DAmix @11:20:53.28 Lysis1.in :DAmix @11:20:53.80 Lysis2.seek 1480uL @11:20:53.84 Lysis1.seek 575uL @11:21:28.90 delay 5 seconds @11:21:33.92 Handoff.select SPR @11:21:34.05 SPR.command \"start 2 14\" @11:21:34.57 SPR stabilized @11:21:34.70 delay 10 seconds @11:21:44.72 SPR.command \"start 2 0\" @11:21:48.44 Waiting up to 11:00 for SPR to enter state 1 @11:23:46.54 SPR.command \"hold_on\" @11:23:47.93 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @11:23:48.36 Lysis2.seek 1180uL @11:23:48.40 Lysis1.seek 275uL @11:24:30.42 delay 15 seconds @11:24:45.44 SPR.command \"hold_off\" @11:24:48.58 Waiting up to 38:00 for SPR to enter state 7 @11:48:45.73 SPR analysis completed @11:48:50.24 Ended log spr21aug1210h.spr.bz2 @11:48:50.25 SPR.command \"stats_off\" @11:48:51.93 -> Cmd.stop @11:48:51.94 Archive.stop @11:48:52.06 Handoff.select :close @11:48:52.19 Gate.power :heat,:OFF @11:48:52.21 Gate.power :cartridge,:OFF @11:48:52.29 Carousel.seek 15 @11:48:52.47 Plunge.seek :locked @11:48:53.36 PinPort.in :unlocking @11:48:53.42 PinPort.seek :clear @11:48:53.96 Archive.in :unlocking @11:48:54.07 Archive.seek :clear @11:48:54.15 Air.in :unlocking @11:48:54.26 Air.seek :clear @11:48:54.34 Lysis1.in :unlocking @11:48:54.47 Lysis1.seek :clear @11:48:54.54 Lysis2.in :unlocking @11:48:54.61 Lysis2.seek :clear @11:48:58.18 Gate.power :motor,:OFF @11:48:58.20 Gate.power :core,:OFF @11:48:58.22 Gate.power :analytic1,:OFF @11:48:58.24 Gate.power :analytic2,:OFF @11:48:58.27 Gate.power :analytic3,:OFF @11:48:58.29 Gate.power :main,:OFF @11:48:58.31 Safely stopped and ready to power off