##-- file opened 2021-08-10T22:38:57.556Z
@18:38:57.93 -> showStatus
@18:38:59.54 -> Cmd.loadCartridge -4
@18:38:59.59 Gate.power :main,:ON,limit to 5000mA
@18:38:59.61 Gate.power :analytic1,:ON,limit to 5000mA
@18:38:59.63 Gate.power :analytic2,:ON,limit to 5000mA
@18:38:59.65 Gate.power :analytic3,:ON,limit to 5000mA
@18:38:59.79 Powering core bus
@18:39:02.07 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@18:39:02.88 <LRAUV> Archive.configure :default
@18:39:03.00 Air.configure :default
@18:39:03.11 Lysis1.configure :default
@18:39:03.22 Lysis2.configure :default
@18:39:03.34 Plunge.configure :default
@18:39:03.47 SamplePump.configure :default
@18:39:03.62 ExtraPump.configure :default
@18:39:03.73 Toroid.configure :default
@18:39:03.85 Carousel.configure :default
@18:39:03.99 Pressure.configure :default
@18:39:04.10 PinPort.configure :default
@18:39:04.16 All elf controllers running firmware version 4.741
@18:39:04.18 Gate.power :motor,:ON,limit to 5000mA
@18:39:04.43 <Archive_home> Archive.in :default
@18:39:04.62 Archive.home.jog 0
@18:39:04.76 <Toroid_home> Toroid.in :default
@18:39:04.84 <Plunge_home> Plunge.in :default
@18:39:04.87 <Lysis1_home> Lysis1.in :default
@18:39:04.92 <PinPort_home> PinPort.in :default
@18:39:04.98 <Lysis2_home> Lysis2.in :default
@18:39:05.02 <Air_home> Air.in :default
@18:39:05.20 <Toroid_home> Toroid.home.jog 0
@18:39:05.22 <Plunge_home> Plunge.home.jog 0
@18:39:05.25 <PinPort_home> PinPort.home.jog 0
@18:39:05.28 <Lysis1_home> Lysis1.home.jog 0
@18:39:05.30 <Air_home> Air.home.jog 0
@18:39:05.33 <Lysis2_home> Lysis2.home.jog 0
@18:39:05.72 <Archive_home> Archive.in :homing
@18:39:05.91 <Toroid_home> Toroid.in :homing
@18:39:05.95 <Plunge_home> Plunge.in :homing
@18:39:06.00 <Archive_home> Archive.jog 1396
@18:39:06.06 <Lysis1_home> Lysis1.in :homing
@18:39:06.08 <PinPort_home> PinPort.in :homing
@18:39:06.23 <Toroid_home> Toroid.jog 617
@18:39:06.31 <Plunge_home> Plunge.jog 1552
@18:39:06.34 <PinPort_home> PinPort.jog -108
@18:39:06.45 <Lysis1_home> Lysis1.jog 1410
@18:39:06.57 <Air_home> Air.in :homing
@18:39:06.59 <Lysis2_home> Lysis2.in :homing
@18:39:06.68 <Air_home> Air.jog 1413
@18:39:06.71 <Lysis2_home> Lysis2.jog 1409
@18:39:06.83 <PinPort_home> PinPort.setRawPosition! 0
@18:39:06.87 PinPort.seek :home
@18:39:06.96 PinPort.in :default
@18:39:07.35 <Archive_home> Archive.setRawPosition! 50
@18:39:07.40 Archive.seek :home
@18:39:07.62 <Plunge_home> Plunge.setRawPosition! 50
@18:39:07.64 <Lysis1_home> Lysis1.setRawPosition! 50
@18:39:07.72 <Plunge_home> Plunge.seek :home
@18:39:07.78 <Lysis1_home> Lysis1.seek :home
@18:39:07.94 <Lysis2_home> Lysis2.setRawPosition! 50
@18:39:07.96 <Air_home> Air.setRawPosition! 50
@18:39:07.99 <Archive_home> Archive.in :default
@18:39:08.08 <Lysis2_home> Lysis2.seek :home
@18:39:08.15 <Air_home> Air.seek :home
@18:39:08.29 <Plunge_home> Plunge.in :default
@18:39:08.32 <Lysis1_home> Lysis1.in :default
@18:39:08.47 <Lysis2_home> Lysis2.in :default
@18:39:08.55 <Toroid_home> Toroid.setRawPosition! 0
@18:39:08.57 <Air_home> Air.in :default
@18:39:08.64 <Toroid_home> Toroid.seek :home
@18:39:08.77 Toroid.in :default
@18:39:09.03 <LRAUV> Toroid.seek :clear
@18:39:10.65 Carousel.in :default
@18:39:10.71 Carousel.home.jog 0
@18:39:11.21 Carousel.in :default
@18:39:11.27 Carousel.seek 33
@18:39:11.43 Selecting Cartridge 32
@18:39:11.68 Carousel.seek 32
@18:39:12.16 Lysis1.in :fast
@18:39:12.22 Lysis1.seek :locked
@18:39:14.83 <Plunge> Plunge.seek :locked
@18:39:14.95 <Air> Air.in :fast
@18:39:15.01 Air.seek :locked
@18:39:17.61 <LRAUV> Powering cartridge bus
@18:39:20.25 Gate.setIR 1750,5000,0,6
@18:39:20.38 Gate.setIR 0,5000,0,6
@18:39:20.43 Gate.power :cartridge,:OFF
@18:39:20.97 Mounted archive Cartridge
@18:39:39.12 -> Cmd.startFiltering
@18:39:39.57 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@18:39:39.58 SamplePump.setPosition! 0ml
@18:39:39.63 Bypass.open
@18:39:41.15 Intake.open
@18:39:41.97 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@18:39:44.53 Vacuum tare is 51% of full scale
@18:39:44.66 Pressure.in :default
@18:39:44.72 Pressure.hold
@18:39:44.88 SamplePump.in :prime
@18:39:44.95 SamplePump.seek 10ml,59 seconds
@18:39:54.22 Pressure.stop
@18:39:54.27 SamplePump.setPosition! 0ml
@18:39:54.32 Exhaust.open
@18:39:55.79 <closeBypass> Bypass.close
@18:39:57.35 <FILTERING> Pressure.in :default
@18:39:57.42 Pressure.hold
@18:39:57.64 SamplePump.in :prime
@18:39:57.70 SamplePump.seek 100ml,5:16
@18:42:02.81 Pressure.stop
@18:42:02.87 Bypass.open
@18:42:03.63 Intake.close
@18:42:04.03 Toroid.in :hold
@18:42:04.09 Toroid.seek :crack
@18:42:05.60 Toroid.seek :cartridge
@18:42:06.68 Toroid.in :default
@18:42:06.74 Toroid.seek :clear
@18:42:08.70 Pressurizing cartridge to 26psi above 12.4psia
@18:42:08.72 SamplePump.setPosition! 0ml
@18:42:08.76 Intake.open
@18:42:09.51 Bypass.close
@18:42:10.25 Exhaust.close
@18:42:11.14 Pressure.in :default
@18:42:11.20 Pressure.hold
@18:42:11.37 SamplePump.in :charge
@18:42:11.44 SamplePump.seek 2.3ml,42 seconds
@18:42:18.95 Pressure.stop
@18:42:19.01 Intake.close
@18:42:20.26 Pressurized cartridge with 0.250ml to 27.2psi
@18:42:20.27 Isolated @32.8psia (20.4psi)
@18:42:20.29 Verifying cartridge holds at least 0.75..0.85 of 32.8psia
@18:42:20.32 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@18:42:24.94 Sampling 1000.0ml
@18:42:24.96 SamplePump.setPosition! 0ml
@18:42:25.01 Exhaust.open
@18:42:25.73 Intake.open
@18:42:26.61 Pressure.in :default
@18:42:26.67 Pressure.hold
@18:42:26.89 SamplePump.in :sample
@18:42:26.96 SamplePump.seek 1000ml,1:23:53
@18:42:29.65 <sampling> Cartridge 32 filtering 1/1000ml at 0.30ml/s with 21.4psi vacuum
@18:43:20.14 <FILTERING> Flow averaged 0.22ml/s over 54 seconds
@18:43:20.17 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi
@18:43:21.00 Relieved excess vacuum in .9 seconds
@18:43:21.02 SamplePump.seek 1000ml,1:22:54
@18:43:22.83 Flow estimate reduced to 0.18ml/s
@18:43:22.86 <closeIntake> Intake.close
@18:43:22.91 <closeExhaust> Exhaust.close
@18:43:23.10 <FILTERING> Toroid.in :hold
@18:43:23.16 Toroid.seek :bypass
@18:43:24.79 Toroid.in :default
@18:43:24.85 Toroid.seek :clear
@18:43:26.47 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@18:43:26.48 Pressure.stop
@18:43:26.67 Sampled  12.2ml
@18:43:28.61 <LRAUV> -> Cmd.startProcessing
@18:43:28.98 <PROCESSING> Plunge.seek :reagent
@18:43:29.82 Sample loop closed. Ready to Deliver Reagents
@18:43:29.83 Archive.seek :sealed
@18:43:34.10 Archive.seek :evac
@18:43:48.04 delay 1:00
@18:44:48.07 Archive.seek :clear
@18:45:05.79 RNAlater soak for 10:00
@18:55:05.82 Archive.seek :tVent
@18:55:08.40 delay 1 second
@18:55:09.42 Archive.seek :evac
@18:55:25.04 delay 10 seconds
@18:55:35.07 Archive.seek :tVent
@18:55:50.69 delay 1 second
@18:55:51.71 Archive.seek :evac
@18:56:07.34 delay 10 seconds
@18:56:17.37 Sample preserved and evacuated
@18:56:18.32 <LRAUV> -> Cmd.stop
@18:56:18.46 Carousel.seek 32
@18:56:18.63 Plunge.seek :locked
@18:56:19.58 <Archive> Archive.in :unlocking
@18:56:19.71 Archive.seek :clear
@18:56:19.75 <Air> Air.in :unlocking
@18:56:19.90 Air.seek :clear
@18:56:19.98 <Lysis1> Lysis1.in :unlocking
@18:56:20.10 Lysis1.seek :clear
@18:56:23.91 <LRAUV> Gate.power :motor,:OFF
@18:56:23.93 Gate.power :core,:OFF
@18:56:23.95 Gate.power :analytic1,:OFF
@18:56:23.97 Gate.power :analytic2,:OFF
@18:56:23.99 Gate.power :analytic3,:OFF
@18:56:24.01 Gate.power :main,:OFF
@18:56:24.03 Safely stopped and ready to power off
##-- file closed 2021-08-10T23:05:22.511Z

##-- file opened 2021-08-10T23:05:22.513Z
@19:05:22.61 -> showStatus
@19:05:24.69 -> Cmd.loadCartridge -18
@19:05:24.76 Gate.power :main,:ON,limit to 5000mA
@19:05:24.78 Gate.power :analytic1,:ON,limit to 5000mA
@19:05:24.80 Gate.power :analytic2,:ON,limit to 5000mA
@19:05:24.82 Gate.power :analytic3,:ON,limit to 5000mA
@19:05:24.96 Powering core bus
@19:05:27.24 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@19:05:28.05 <LRAUV> Archive.configure :default
@19:05:28.17 Air.configure :default
@19:05:28.28 Lysis1.configure :default
@19:05:28.38 Lysis2.configure :default
@19:05:28.50 Plunge.configure :default
@19:05:28.63 SamplePump.configure :default
@19:05:28.78 ExtraPump.configure :default
@19:05:28.90 Toroid.configure :default
@19:05:29.03 Carousel.configure :default
@19:05:29.15 Pressure.configure :default
@19:05:29.27 PinPort.configure :default
@19:05:29.33 All elf controllers running firmware version 4.741
@19:05:29.35 Gate.power :motor,:ON,limit to 5000mA
@19:05:29.52 <Archive_home> Archive.in :default
@19:05:29.76 Archive.home.jog 0
@19:05:29.91 <Toroid_home> Toroid.in :default
@19:05:29.99 <Plunge_home> Plunge.in :default
@19:05:30.03 <Lysis1_home> Lysis1.in :default
@19:05:30.10 <Air_home> Air.in :default
@19:05:30.13 <Lysis2_home> Lysis2.in :default
@19:05:30.19 <PinPort_home> PinPort.in :default
@19:05:30.31 <Toroid_home> Toroid.home.jog 0
@19:05:30.37 <Lysis1_home> Lysis1.home.jog 0
@19:05:30.40 <Plunge_home> Plunge.home.jog 0
@19:05:30.43 <Lysis2_home> Lysis2.home.jog 0
@19:05:30.45 <Air_home> Air.home.jog 0
@19:05:30.52 <PinPort_home> PinPort.home.jog 0
@19:05:30.95 <Archive_home> Archive.in :homing
@19:05:31.11 <Toroid_home> Toroid.in :homing
@19:05:31.18 <Lysis2_home> Lysis2.in :homing
@19:05:31.22 <Archive_home> Archive.jog 175
@19:05:31.25 <Lysis1_home> Lysis1.in :homing
@19:05:31.28 <Air_home> Air.in :homing
@19:05:31.32 <PinPort_home> PinPort.in :homing
@19:05:31.36 <Plunge_home> Plunge.in :homing
@19:05:31.59 <Lysis2_home> Lysis2.jog 1410
@19:05:31.62 <Toroid_home> Toroid.jog 612
@19:05:31.65 <Plunge_home> Plunge.jog 1570
@19:05:31.68 <Air_home> Air.jog 1409
@19:05:31.71 <Lysis1_home> Lysis1.jog 1412
@19:05:31.76 <PinPort_home> PinPort.jog -109
@19:05:32.00 <Archive_home> Archive.setRawPosition! 50
@19:05:32.05 Archive.seek :home
@19:05:32.13 <PinPort_home> PinPort.setRawPosition! 0
@19:05:32.18 PinPort.seek :home
@19:05:32.27 PinPort.in :default
@19:05:32.36 <Archive_home> Archive.in :default
@19:05:32.89 <Lysis2_home> Lysis2.setRawPosition! 50
@19:05:32.94 Lysis2.seek :home
@19:05:33.04 <Air_home> Air.setRawPosition! 50
@19:05:33.07 <Lysis1_home> Lysis1.setRawPosition! 50
@19:05:33.14 Lysis1.seek :home
@19:05:33.17 <Air_home> Air.seek :home
@19:05:33.30 <Plunge_home> Plunge.setRawPosition! 50
@19:05:33.32 <Lysis2_home> Lysis2.in :default
@19:05:33.39 <Plunge_home> Plunge.seek :home
@19:05:33.52 <Air_home> Air.in :default
@19:05:33.54 <Lysis1_home> Lysis1.in :default
@19:05:33.70 <Plunge_home> Plunge.in :default
@19:05:33.87 <Toroid_home> Toroid.setRawPosition! 0
@19:05:33.92 Toroid.seek :home
@19:05:34.01 Toroid.in :default
@19:05:34.15 <LRAUV> Toroid.seek :clear
@19:05:35.76 Carousel.in :default
@19:05:35.82 Carousel.home.jog 0
@19:05:36.32 Carousel.in :default
@19:05:36.38 Carousel.seek 32
@19:05:36.52 Selecting Cartridge 31
@19:05:36.76 Carousel.seek 31
@19:05:37.25 Lysis1.in :fast
@19:05:37.32 Lysis1.seek :locked
@19:05:39.91 <Plunge> Plunge.seek :locked
@19:05:40.03 <Air> Air.in :fast
@19:05:40.09 Air.seek :locked
@19:05:42.70 <LRAUV> Powering cartridge bus
@19:05:45.32 Gate.setIR 1750,5000,0,6
@19:05:45.45 Gate.setIR 0,5000,0,6
@19:05:45.51 PuckHeater.configure :default
@19:05:45.57 Gate.power :heat,:ON,limit to 5000mA
@19:05:46.23 Mounted lysis Cartridge board#Q05086
@19:05:46.27 Using default puck heat loss curve for HeatingAccelInCarousel
@19:06:05.15 -> Cmd.startFiltering
@19:06:05.61 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@19:06:05.62 SamplePump.setPosition! 0ml
@19:06:05.66 Bypass.open
@19:06:07.15 Intake.open
@19:06:07.93 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@19:06:10.50 Vacuum tare is 51% of full scale
@19:06:10.63 Pressure.in :default
@19:06:10.69 Pressure.hold
@19:06:10.93 SamplePump.in :prime
@19:06:11.00 SamplePump.seek 10ml,59 seconds
@19:06:20.25 Pressure.stop
@19:06:20.30 SamplePump.setPosition! 0ml
@19:06:20.35 Exhaust.open
@19:06:21.78 <closeBypass> Bypass.close
@19:06:23.36 <FILTERING> Pressure.in :default
@19:06:23.42 Pressure.hold
@19:06:23.64 SamplePump.in :prime
@19:06:23.71 SamplePump.seek 100ml,5:16
@19:08:29.05 Pressure.stop
@19:08:29.11 Bypass.open
@19:08:29.84 Intake.close
@19:08:30.67 Toroid.in :hold
@19:08:30.73 Toroid.seek :crack
@19:08:32.24 Toroid.seek :cartridge
@19:08:33.33 Toroid.in :default
@19:08:33.39 Toroid.seek :clear
@19:08:35.35 Pressurizing cartridge to 14psi above 12.2psia
@19:08:35.36 SamplePump.setPosition! 0ml
@19:08:35.40 Intake.open
@19:08:36.15 Bypass.close
@19:08:36.87 Exhaust.close
@19:08:37.69 Pressure.in :default
@19:08:37.75 Pressure.hold
@19:08:38.31 SamplePump.in :charge
@19:08:38.38 SamplePump.seek 2.3ml,41 seconds
@19:08:48.72 Pressure.stop
@19:08:48.77 Intake.close
@19:08:49.61 Pressurized cartridge with 0.476ml to 14.3psi
@19:08:49.62 Isolated @22.0psia (9.8psi)
@19:08:49.63 Verifying cartridge holds at least 0.75..0.85 of 22.0psia
@19:08:49.66 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@19:08:55.24 Cartridge pressure fell 16% to 18.5psia
@19:08:55.34 Toroid.in :hold
@19:08:55.40 Toroid.seek :bypass
@19:08:57.03 Toroid.in :default
@19:08:57.09 Toroid.seek :clear
@19:08:58.77 Cartridge::Sampler::Leaky in FILTERING -- Retained only 18.5psia
@19:08:58.78 Retry #1 of 2
@19:08:58.80 Cmd::SpareCartridge in FILTERING -- 0 microSPR_phyto spares remaining
@19:08:58.86 Selecting Cartridge 29
@19:08:58.99 Gate.power :heat,:OFF
@19:08:59.01 Gate.power :cartridge,:OFF
@19:08:59.27 <Air> Air.in :unlocking
@19:08:59.39 Air.seek :clear
@19:09:00.79 <Lysis1> Lysis1.in :unlocking
@19:09:00.92 Lysis1.seek :clear
@19:09:00.99 <Plunge> Plunge.in :unlocking
@19:09:01.06 Plunge.seek :clear
@19:09:02.24 <FILTERING> Carousel.seek 29
@19:09:02.93 Lysis1.in :fast
@19:09:02.99 Lysis1.seek :locked
@19:09:05.64 <Plunge> Plunge.in :default
@19:09:05.72 Plunge.seek :locked
@19:09:05.84 <Air> Air.in :fast
@19:09:05.90 Air.seek :locked
@19:09:08.50 <FILTERING> Powering cartridge bus
@19:09:11.12 Gate.setIR 1750,5000,0,6
@19:09:11.25 Gate.setIR 0,5000,0,6
@19:09:11.31 PuckHeater.configure :default
@19:09:11.36 Gate.power :heat,:ON,limit to 5000mA
@19:09:12.03 Mounted lysis Cartridge board#Q05057
@19:09:12.06 Using default puck heat loss curve for HeatingAccelInCarousel
@19:09:12.26 Bypass.open
@19:09:13.06 Toroid.in :hold
@19:09:13.12 Toroid.seek :crack
@19:09:14.64 Toroid.seek :cartridge
@19:09:15.74 Toroid.in :default
@19:09:15.80 Toroid.seek :clear
@19:09:17.60 Exhaust.open
@19:09:18.49 Pressurizing cartridge to 14psi above 13.3psia
@19:09:18.51 SamplePump.setPosition! 0ml
@19:09:18.55 Intake.open
@19:09:19.29 Bypass.close
@19:09:20.03 Exhaust.close
@19:09:20.86 Pressure.in :default
@19:09:20.92 Pressure.hold
@19:09:21.09 SamplePump.seek 2.3ml,41 seconds
@19:09:30.00 Pressure.stop
@19:09:30.06 Intake.close
@19:09:30.89 Pressurized cartridge with 0.353ml to 14.3psi
Isolated @22.9psia (9.6psi)
@19:09:30.91 Verifying cartridge holds at least 0.75..0.85 of 22.9psia
@19:09:30.94 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@19:09:35.56 Sampling 50.0ml
@19:09:35.58 SamplePump.setPosition! 0ml
@19:09:35.63 Exhaust.open
@19:09:36.34 Intake.open
@19:09:37.20 Pressure.in :default
@19:09:37.26 Pressure.hold
@19:09:37.48 SamplePump.in :sample
@19:09:37.55 SamplePump.seek 50ml,3:51
@19:09:40.25 <sampling> Cartridge 29 filtering 1/50ml at 0.34ml/s with 15.7psi vacuum
@19:10:40.24 Cartridge 29 filtering 19/50ml at 0.31ml/s with 13.8psi vacuum
@19:11:40.23 Cartridge 29 filtering 37/50ml at 0.30ml/s with 13.5psi vacuum
@19:12:25.44 <FILTERING> Pressure.stop
@19:12:25.56 <closeIntake> Intake.close
@19:12:25.61 <closeExhaust> Exhaust.close
@19:12:25.76 <FILTERING> Toroid.in :hold
@19:12:25.82 Toroid.seek :bypass
@19:12:27.43 Toroid.in :default
@19:12:27.50 Toroid.seek :clear
@19:12:29.11 Sampled  50.0ml
@19:12:30.58 <LRAUV> -> Cmd.startProcessing
@19:12:32.09 <PROCESSING> SPR.command \"read_sequences\"
@19:12:32.74 SPR.command \"stats_on\"
@19:12:32.78 SPR.command \"start 0 1\"
@19:12:33.59 <SPRlogger> SPR.start /var/log/esp/spr21aug1019h.spr.bz2
@19:12:36.08 <PROCESSING> Handoff.select :waste
@19:12:42.77 Syringe tare is 19% of full scale
@19:12:42.79 PinPort.in :locking
@19:12:42.86 PinPort.seek :locked
@19:12:43.33 <posArchive> Archive.in :fast
@19:12:43.37 <posLyS2> Lysis2.in :fast
@19:12:43.48 <PROCESSING> Lysis1.seek 1350uL
@19:12:43.51 <posArchive> Archive.seek :full
@19:12:43.56 <posLyS2> Lysis2.seek :full
@19:12:45.68 <PROCESSING> Plunge.seek :reagent
@19:12:46.60 Evacuating puck with filter bubble point of 24psi
@19:12:46.62 PinPort.seek :locked
@19:12:46.71 Handoff.select :waste
@19:12:53.29 Air syringe pumping up to 1 stroke to reach 8psi
@19:12:53.30 Air stroke #1 of 1
@19:12:53.39 Air.in :pressurize
@19:12:53.45 Air.seek :pressurized
@19:13:04.70 0.1psi after pressurize
@19:13:04.72 Air.in :compress
@19:13:04.79 Air.seek :evac
@19:13:08.87 0.7psi after compress
@19:13:21.45 0.5psi after compress (stable)
@19:13:21.47 Air.in :default
@19:13:21.55 Air.hold
@19:13:21.72 at 0.5psi Opened to waste
@19:13:21.73 Handoff.select :blocked
@19:13:21.87 Archive syringe pumping up to 8 strokes to reach 24psi
@19:13:21.88 Archive stroke #1 of 8
@19:13:21.96 Archive.in :pressurize
@19:13:22.02 Archive.seek :pressurized
@19:13:34.78 2.9psi after pressurize
@19:13:34.80 Archive.in :compress
@19:13:34.87 Archive.seek :empty
@19:13:50.75 10.3psi after compress
@19:14:15.34 18.5psi after compress (stable)
@19:14:15.38 Archive.in :gulp
@19:14:15.46 Archive.seek :topVent
@19:14:22.66 18.9psi after gulp
@19:14:35.24 18.8psi after gulp (stable)
@19:14:35.26 Archive stroke #2 of 8
@19:14:35.35 Archive.in :pressurize
@19:14:35.41 Archive.seek :pressurized
@19:14:48.15 18.7psi after pressurize
@19:14:48.16 Archive.in :compress
@19:14:48.24 Archive.seek :empty
@19:14:57.00 Reached 24.1 of 24.0psi after pumping 3308uL (1.91 strokes)
@19:14:57.02 Archive.in :default
@19:14:57.10 Archive.hold
@19:14:57.16 Handoff.select :waste
@19:15:11.35 delay 1:00
@19:16:11.37 Handoff.select :blocked
@19:16:34.05 Handoff.select :waste
@19:16:34.17 0.3psi at evacEnd
@19:16:34.18 Handoff.select :blocked
@19:16:34.31 Archive.in :fast
@19:16:34.38 Archive.seek :topVent
@19:16:47.58 Stable @0.3psi after Handoff blocked
@19:16:47.60 Archive syringe pumping up to 8 strokes to reach 15psi
@19:16:47.62 Archive stroke #1 of 8
@19:16:47.72 Archive.in :pressurize
@19:16:47.78 Archive.seek :pressurized
@19:17:00.52 0.5psi after pressurize
@19:17:00.53 Archive.in :compress
@19:17:00.61 Archive.seek :empty
@19:17:16.49 13.9psi after compress
@19:17:29.07 14.8psi after compress (stable)
@19:17:29.08 Archive.in :gulp
@19:17:29.15 Archive.seek :topVent
@19:17:36.35 15.1psi after gulp
@19:17:48.92 15.0psi after gulp (stable)
@19:17:48.93 Archive stroke #2 of 8
@19:17:49.03 Archive.in :pressurize
@19:17:49.09 Archive.seek :pressurized
@19:18:01.81 15.0psi after pressurize
@19:18:01.83 Archive.in :compress
@19:18:01.90 Archive.seek :empty
@19:18:04.55 Reached 15.3 of 15.3psi after pumping 3177uL (1.84 strokes)
@19:18:04.56 Archive.in :default
@19:18:04.63 Archive.hold
@19:18:04.70 Handoff.select :waste
@19:18:13.89 delay 1:00
@19:19:13.91 Handoff.select :blocked
@19:19:36.59 Handoff.select :waste
@19:19:36.71 0.3psi at evacEnd
@19:19:36.73 Air.seek :fullVent
@19:19:38.18 Archive.in :fast
@19:19:38.24 Archive.seek :topVent
@19:19:44.38 Seawater evacuated, Presure above/below filter released. Lysis begins
@19:19:44.47 Lysis1.in :slow
@19:19:44.54 Lysis1.seek 1050uL
@19:20:14.55 delay 5 seconds
@19:20:19.57 Handoff.select :blocked
@19:20:19.73 Lysis1.seek 450uL
@19:21:19.67 delay 3 seconds
@19:21:22.74 Air.in to mix into 1.37806 parts Lysis1
@19:21:23.27 <Airmixer> Air.seek :evac
@19:21:23.31 <PROCESSING> Lysis1.seek 300uL
@19:21:38.42 Lysis1.seek :empty
@19:22:08.51 Lysis buffer delivered @16.0psi
@19:22:08.52 Polling Can every 2:00
@19:22:09.07 PuckHeater.configure :default #ambient 25.1C
@19:22:09.11 PuckHeater.seek 79.6C
@19:22:59.66 PuckHeater.hold 2:00,79.6C
@19:24:59.64 Polling Can every 2:00
@19:25:00.19 PuckHeater.configure :default #ambient 25.1C
@19:25:00.23 PuckHeater.hold 30 seconds,78.6C
@19:25:30.40 Polling Can every 2:00
@19:25:30.95 PuckHeater.hold 30 seconds,78.1C
@19:26:01.12 Polling Can every 2:00
@19:26:01.67 PuckHeater.configure :default #ambient 25.1C
@19:26:01.72 PuckHeater.hold 10:00,77.5C
@19:36:01.46 Lysis complete. Syringe pressure @22.6psi
@19:36:01.47 Handoff.select :blocked
@19:36:01.52 Archive syringe pumping up to 5 strokes to reach 40psi
@19:36:01.53 Archive stroke #1 of 5
@19:36:01.63 Archive.in :pressurize
@19:36:01.69 Archive.seek :pressurized
@19:36:14.42 22.2psi after pressurize
@19:36:14.43 Archive.in :compress
@19:36:14.51 Archive.seek :empty
@19:36:22.60 Reached 40.3 of 40.0psi after pumping 1564uL (0.90 strokes)
@19:36:22.61 Archive.in :default
@19:36:22.68 Archive.hold
@19:36:22.75 delay 2 seconds
@19:36:24.77 Archive.in :fast
@19:36:24.83 Archive.seek :full
@19:36:31.46 Lysis1.seek 138uL
@19:36:45.34 delay 1 second
@19:36:46.37 <fillArchive> Archive.seek :full
@19:36:46.40 <PROCESSING> Lysis1.seek :empty
@19:37:00.74 Initial air pressure @48.2psi
@19:37:00.81 Lysis1.in :extract
@19:37:00.90 Lysis1.seek 600uL,nil
@19:37:06.10 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@19:37:06.12 Archive stroke #1 of 6
@19:37:06.20 Archive.in :pressurize
@19:37:06.27 Archive.seek :pressurized
@19:37:19.09 15.4psi after pressurize
@19:37:19.11 Archive.in :compress
@19:37:19.20 Archive.seek :empty
@19:37:28.18 Archive.in :default
@19:37:28.23 Archive.hold
@19:37:28.44 <PROCESSING> Initial 600uL lysate @17.9psi
@19:37:28.45 Handoff.select :blocked
@19:37:28.55 Initial air pressure @18.6psi
@19:37:28.63 Lysis1.seek 825uL,nil
@19:37:29.83 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@19:37:29.84 Archive stroke #1 of 6
@19:37:29.93 Archive.in :compress
@19:37:30.00 Archive.seek :empty
@19:37:34.89 Archive.in :default
@19:37:34.94 Archive.hold
@19:37:35.23 <PROCESSING> Recovered 825uL lysate @17.2psi
@19:37:35.27 Air.in :halfSpeed
@19:37:35.34 Air.seek :fullVent
@19:37:37.58 Puck Top Vented, bottom @3.4psi
@19:37:37.59 Handoff.select :waste
@19:37:37.71 delay 5 seconds
@19:37:42.77 Handoff open to waste, bottom @0.0psi
@19:37:42.80 Air.in :default
@19:37:42.88 Air.seek :evac
@19:37:44.31 Lysis2.in :DAmix
@19:37:44.41 Lysis1.in :DAmix
@19:37:44.94 <Lysis2mixer> Lysis2.seek 1480uL
@19:37:45.01 <PROCESSING> Lysis1.seek 575uL
@19:38:20.03 delay 5 seconds
@19:38:25.05 Handoff.select SPR
@19:38:25.18 SPR.command \"start 2 14\"
@19:38:25.84 SPR stabilized
@19:38:25.97 delay 10 seconds
@19:38:35.99 SPR.command \"start 2 0\"
@19:38:39.20 Waiting up to 11:00 for SPR to enter state 1
@19:40:36.86 SPR.command \"hold_on\"
@19:40:38.26 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2
@19:40:38.73 <Lysis2mixer> Lysis2.seek 1180uL
@19:40:38.79 <PROCESSING> Lysis1.seek 275uL
@19:41:20.82 delay 15 seconds
@19:41:35.84 SPR.command \"hold_off\"
@19:41:38.91 Waiting up to 38:00 for SPR to enter state 7
@20:05:36.04 SPR analysis completed
@20:05:39.99 <SPRlogger> Ended log spr21aug1019h.spr.bz2
@20:05:40.00 <PROCESSING> SPR.command \"stats_off\"
@20:05:41.47 <LRAUV> -> Cmd.stop
@20:05:41.49 Archive.stop
@20:05:41.61 <closeHandoff> Handoff.select :close
@20:05:41.74 <LRAUV> Gate.power :heat,:OFF
@20:05:41.76 Gate.power :cartridge,:OFF
@20:05:41.84 Carousel.seek 29
@20:05:42.02 Plunge.seek :locked
@20:05:42.90 PinPort.in :unlocking
@20:05:42.97 PinPort.seek :clear
@20:05:43.51 <Archive> Archive.in :unlocking
@20:05:43.64 Archive.seek :clear
@20:05:43.67 <Air> Air.in :unlocking
@20:05:43.80 Air.seek :clear
@20:05:43.88 <Lysis1> Lysis1.in :unlocking
@20:05:44.01 Lysis1.seek :clear
@20:05:44.08 <Lysis2> Lysis2.in :unlocking
@20:05:44.15 Lysis2.seek :clear
@20:05:47.78 <LRAUV> Gate.power :motor,:OFF
@20:05:47.80 Gate.power :core,:OFF
@20:05:47.82 Gate.power :analytic1,:OFF
@20:05:47.84 Gate.power :analytic2,:OFF
@20:05:47.87 Gate.power :analytic3,:OFF
@20:05:47.89 Gate.power :main,:OFF
@20:05:47.91 Safely stopped and ready to power off
##-- file closed 2021-08-11T14:01:07.322Z