##-- file opened 2021-08-09T15:39:47.076Z
@11:39:47.67 -> showStatus
@11:39:49.69 -> Cmd.loadCartridge -4
@11:39:49.75 Gate.power :main,:ON,limit to 5000mA
@11:39:49.76 Gate.power :analytic1,:ON,limit to 5000mA
@11:39:49.78 Gate.power :analytic2,:ON,limit to 5000mA
@11:39:49.80 Gate.power :analytic3,:ON,limit to 5000mA
@11:39:49.94 Powering core bus
@11:39:52.23 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@11:39:52.68 <LRAUV> Archive.configure :default
@11:39:52.80 Air.configure :default
@11:39:52.91 Lysis1.configure :default
@11:39:53.01 Lysis2.configure :default
@11:39:53.13 Plunge.configure :default
@11:39:53.61 SamplePump.configure :default
@11:39:53.76 ExtraPump.configure :default
@11:39:53.86 Toroid.configure :default
@11:39:53.99 Carousel.configure :default
@11:39:54.11 Pressure.configure :default
@11:39:54.23 PinPort.configure :default
@11:39:54.29 All elf controllers running firmware version 4.741
@11:39:54.32 Gate.power :motor,:ON,limit to 5000mA
@11:39:54.45 <Archive_home> Archive.in :default
@11:39:54.78 Archive.home.jog 0
@11:39:54.93 <Toroid_home> Toroid.in :default
@11:39:55.01 <Plunge_home> Plunge.in :default
@11:39:55.04 <Lysis1_home> Lysis1.in :default
@11:39:55.10 <Air_home> Air.in :default
@11:39:55.15 <Lysis2_home> Lysis2.in :default
@11:39:55.19 <PinPort_home> PinPort.in :default
@11:39:55.38 <Toroid_home> Toroid.home.jog 0
@11:39:55.41 <Lysis1_home> Lysis1.home.jog 0
@11:39:55.44 <Plunge_home> Plunge.home.jog 0
@11:39:55.46 <Air_home> Air.home.jog 0
@11:39:55.49 <PinPort_home> PinPort.home.jog 0
@11:39:55.51 <Lysis2_home> Lysis2.home.jog 0
@11:39:55.99 <Archive_home> Archive.in :homing
@11:39:56.18 <Toroid_home> Toroid.in :homing
@11:39:56.22 <Plunge_home> Plunge.in :homing
@11:39:56.25 <Air_home> Air.in :homing
@11:39:56.29 <PinPort_home> PinPort.in :homing
@11:39:56.33 <Lysis1_home> Lysis1.in :homing
@11:39:56.36 <Archive_home> Archive.jog 1367
@11:39:56.41 <Lysis2_home> Lysis2.in :homing
@11:39:56.62 <Toroid_home> Toroid.jog 620
@11:39:56.66 <Air_home> Air.jog 1414
@11:39:56.70 <Plunge_home> Plunge.jog 1534
@11:39:56.72 <Lysis1_home> Lysis1.jog 151
@11:39:56.75 <PinPort_home> PinPort.jog -109
@11:39:56.79 <Lysis2_home> Lysis2.jog 1428
@11:39:57.13 <PinPort_home> PinPort.setRawPosition! 0
@11:39:57.18 PinPort.seek :home
@11:39:57.29 PinPort.in :default
@11:39:57.36 <Lysis1_home> Lysis1.setRawPosition! 50
@11:39:57.41 Lysis1.seek :home
@11:39:57.70 Lysis1.in :default
@11:39:57.73 <Archive_home> Archive.setRawPosition! 50
@11:39:57.80 Archive.seek :home
@11:39:57.98 <Air_home> Air.setRawPosition! 50
@11:39:58.03 Air.seek :home
@11:39:58.15 <Lysis2_home> Lysis2.setRawPosition! 50
@11:39:58.18 <Plunge_home> Plunge.setRawPosition! 50
@11:39:58.21 <Archive_home> Archive.in :default
@11:39:58.27 <Lysis2_home> Lysis2.seek :home
@11:39:58.34 <Plunge_home> Plunge.seek :home
@11:39:58.50 <Air_home> Air.in :default
@11:39:58.68 <Lysis2_home> Lysis2.in :default
@11:39:58.75 <Plunge_home> Plunge.in :default
@11:39:58.87 <Toroid_home> Toroid.setRawPosition! 0
@11:39:58.92 Toroid.seek :home
@11:39:59.01 Toroid.in :default
@11:39:59.14 <LRAUV> Toroid.seek :clear
@11:40:00.75 Carousel.in :default
@11:40:00.81 Carousel.home.jog 0
@11:40:01.31 Carousel.in :default
@11:40:01.37 Carousel.seek 37
@11:40:01.53 Selecting Cartridge 36
@11:40:01.78 Carousel.seek 36
@11:40:02.26 Lysis1.in :fast
@11:40:02.34 Lysis1.seek :locked
@11:40:04.93 <Plunge> Plunge.seek :locked
@11:40:05.05 <Air> Air.in :fast
@11:40:05.11 Air.seek :locked
@11:40:07.72 <LRAUV> Powering cartridge bus
@11:40:10.34 Gate.setIR 1750,5000,0,6
@11:40:10.47 Gate.setIR 0,5000,0,6
@11:40:10.52 Gate.power :cartridge,:OFF
@11:40:11.06 Mounted archive Cartridge
@11:40:29.30 -> Cmd.startFiltering
@11:40:29.76 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@11:40:29.77 SamplePump.setPosition! 0ml
@11:40:29.81 Bypass.open
@11:40:31.28 Intake.open
@11:40:31.62 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@11:40:34.18 Vacuum tare is 50% of full scale
@11:40:34.32 Pressure.in :default
@11:40:34.38 Pressure.hold
@11:40:34.54 SamplePump.in :prime
@11:40:34.61 SamplePump.seek 10ml,59 seconds
@11:40:43.88 Pressure.stop
@11:40:43.93 SamplePump.setPosition! 0ml
@11:40:43.98 Exhaust.open
@11:40:45.43 <closeBypass> Bypass.close
@11:40:46.98 <FILTERING> Pressure.in :default
@11:40:47.04 Pressure.hold
@11:40:47.25 SamplePump.in :prime
@11:40:47.32 SamplePump.seek 100ml,5:16
@11:42:52.59 Pressure.stop
@11:42:52.65 Bypass.open
@11:42:53.39 Intake.close
@11:42:54.25 Toroid.in :hold
@11:42:54.31 Toroid.seek :crack
@11:42:55.83 Toroid.seek :cartridge
@11:42:56.91 Toroid.in :default
@11:42:56.97 Toroid.seek :clear
@11:42:58.93 Pressurizing cartridge to 26psi above 13.6psia
@11:42:58.94 SamplePump.setPosition! 0ml
@11:42:58.99 Intake.open
@11:42:59.74 Bypass.close
@11:43:00.48 Exhaust.close
@11:43:01.30 Pressure.in :default
@11:43:01.36 Pressure.hold
@11:43:01.52 SamplePump.in :charge
@11:43:01.59 SamplePump.seek 2.3ml,42 seconds
@11:43:08.86 Pressure.stop
@11:43:08.91 Intake.close
@11:43:09.74 Pressurized cartridge with 0.233ml to 26.0psi
@11:43:09.75 Isolated @34.5psia (20.9psi)
@11:43:09.77 Verifying cartridge holds at least 0.75..0.85 of 34.5psia
@11:43:09.80 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@11:43:14.42 Sampling 1000.0ml
@11:43:14.44 SamplePump.setPosition! 0ml
@11:43:14.49 Exhaust.open
@11:43:15.23 Intake.open
@11:43:15.56 Pressure.in :default
@11:43:15.62 Pressure.hold
@11:43:15.84 SamplePump.in :sample
@11:43:15.91 SamplePump.seek 1000ml,1:23:53
@11:43:18.58 <sampling> Cartridge 36 filtering 1/1000ml at 0.34ml/s with 27.8psi vacuum
@11:44:18.59 Cartridge 36 filtering 23/1000ml at 0.38ml/s with 18.9psi vacuum
@11:45:18.59 Cartridge 36 filtering 44/1000ml at 0.31ml/s with 22.5psi vacuum
@11:46:18.59 Cartridge 36 filtering 64/1000ml at 0.37ml/s with 22.2psi vacuum
@11:47:18.59 Cartridge 36 filtering 83/1000ml at 0.35ml/s with 24.8psi vacuum
@11:48:18.59 Cartridge 36 filtering 102/1000ml at 0.31ml/s with 19.0psi vacuum
@11:49:18.59 Cartridge 36 filtering 119/1000ml at 0.31ml/s with 26.0psi vacuum
@11:50:18.59 Cartridge 36 filtering 135/1000ml at 0.22ml/s with 21.8psi vacuum
@11:51:18.59 Cartridge 36 filtering 150/1000ml at 0.29ml/s with 23.6psi vacuum
@11:52:18.59 Cartridge 36 filtering 165/1000ml at 0.25ml/s with 27.4psi vacuum
@11:53:18.59 Cartridge 36 filtering 179/1000ml at 0.24ml/s with 26.6psi vacuum
@11:54:18.59 Cartridge 36 filtering 192/1000ml at 0.24ml/s with 26.2psi vacuum
@11:55:18.59 Cartridge 36 filtering 205/1000ml at 0.20ml/s with 29.9psi vacuum
@11:55:34.10 <FILTERING> Flow averaged 0.28ml/s over 12:19
@11:55:34.13 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi
@11:55:34.70 Relieved excess vacuum in .6 seconds
@11:55:34.73 SamplePump.seek 1000ml,1:06:32
@11:55:43.48 Flow estimate reduced to 0.23ml/s
@11:55:43.51 Waiting up to 20 seconds for vacuum to reduce from 31.0 to 18.0psi
@11:55:44.32 Relieved excess vacuum in .8 seconds
@11:55:44.35 SamplePump.seek 1000ml,1:06:23
@11:55:50.69 Flow estimate reduced to 0.18ml/s
@11:55:50.72 <closeIntake> Intake.close
@11:55:50.77 <closeExhaust> Exhaust.close
@11:55:50.95 <FILTERING> Toroid.in :hold
@11:55:51.01 Toroid.seek :bypass
@11:55:52.64 Toroid.in :default
@11:55:52.70 Toroid.seek :clear
@11:55:54.32 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@11:55:54.33 Pressure.stop
@11:55:54.52 Sampled  211.1ml
@11:55:56.50 <LRAUV> -> Cmd.startProcessing
@11:55:56.87 <PROCESSING> Plunge.seek :reagent
@11:55:57.70 Sample loop closed. Ready to Deliver Reagents
@11:55:57.71 Archive.seek :sealed
@11:56:01.98 Archive.seek :evac
@11:56:15.91 delay 1:00
@11:57:15.94 Archive.seek :clear
@11:57:33.66 RNAlater soak for 10:00
@12:07:33.68 Archive.seek :tVent
@12:07:36.26 delay 1 second
@12:07:37.28 Archive.seek :evac
@12:07:52.89 delay 10 seconds
@12:08:02.92 Archive.seek :tVent
@12:08:18.53 delay 1 second
@12:08:19.55 Archive.seek :evac
@12:08:35.17 delay 10 seconds
@12:08:45.20 Sample preserved and evacuated
@12:08:46.16 <LRAUV> -> Cmd.stop
@12:08:46.30 Carousel.seek 36
@12:08:46.47 Plunge.seek :locked
@12:08:47.42 <Archive> Archive.in :unlocking
@12:08:47.54 Archive.seek :clear
@12:08:47.58 <Air> Air.in :unlocking
@12:08:47.73 Air.seek :clear
@12:08:47.81 <Lysis1> Lysis1.in :unlocking
@12:08:47.92 Lysis1.seek :clear
@12:08:51.75 <LRAUV> Gate.power :motor,:OFF
@12:08:51.77 Gate.power :core,:OFF
@12:08:51.79 Gate.power :analytic1,:OFF
@12:08:51.81 Gate.power :analytic2,:OFF
@12:08:51.83 Gate.power :analytic3,:OFF
@12:08:51.85 Gate.power :main,:OFF
@12:08:51.87 Safely stopped and ready to power off
##-- file closed 2021-08-09T16:17:48.939Z

##-- file opened 2021-08-09T16:17:48.941Z
@12:17:49.72 -> showStatus
@12:17:51.74 -> Cmd.loadCartridge -18
@12:17:51.80 Gate.power :main,:ON,limit to 5000mA
@12:17:51.81 Gate.power :analytic1,:ON,limit to 5000mA
@12:17:51.83 Gate.power :analytic2,:ON,limit to 5000mA
@12:17:51.85 Gate.power :analytic3,:ON,limit to 5000mA
@12:17:52.00 Powering core bus
@12:17:54.28 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@12:17:54.74 <LRAUV> Archive.configure :default
@12:17:54.86 Air.configure :default
@12:17:54.97 Lysis1.configure :default
@12:17:55.07 Lysis2.configure :default
@12:17:55.54 Plunge.configure :default
@12:17:55.67 SamplePump.configure :default
@12:17:55.81 ExtraPump.configure :default
@12:17:55.91 Toroid.configure :default
@12:17:56.04 Carousel.configure :default
@12:17:56.16 Pressure.configure :default
@12:17:56.28 PinPort.configure :default
@12:17:56.34 All elf controllers running firmware version 4.741
@12:17:56.37 Gate.power :motor,:ON,limit to 5000mA
@12:17:56.54 <Archive_home> Archive.in :default
@12:17:56.83 Archive.home.jog 0
@12:17:56.98 <Toroid_home> Toroid.in :default
@12:17:57.06 <Plunge_home> Plunge.in :default
@12:17:57.09 <Lysis1_home> Lysis1.in :default
@12:17:57.13 <Air_home> Air.in :default
@12:17:57.17 <PinPort_home> PinPort.in :default
@12:17:57.20 <Lysis2_home> Lysis2.in :default
@12:17:57.41 <Toroid_home> Toroid.home.jog 0
@12:17:57.45 <Plunge_home> Plunge.home.jog 0
@12:17:57.48 <Lysis1_home> Lysis1.home.jog 0
@12:17:57.51 <PinPort_home> PinPort.home.jog 0
@12:17:57.54 <Lysis2_home> Lysis2.home.jog 0
@12:17:57.56 <Air_home> Air.home.jog 0
@12:17:57.92 <Archive_home> Archive.in :homing
@12:17:58.14 <Toroid_home> Toroid.in :homing
@12:17:58.17 <Plunge_home> Plunge.in :homing
@12:17:58.22 <Archive_home> Archive.jog 161
@12:17:58.28 <Lysis1_home> Lysis1.in :homing
@12:17:58.31 <PinPort_home> PinPort.in :homing
@12:17:58.49 <Toroid_home> Toroid.jog 619
@12:17:58.53 <Plunge_home> Plunge.jog 1572
@12:17:58.56 <PinPort_home> PinPort.jog -108
@12:17:58.59 <Lysis1_home> Lysis1.jog 1411
@12:17:58.79 <Air_home> Air.in :homing
@12:17:58.81 <Lysis2_home> Lysis2.in :homing
@12:17:58.92 Lysis2.jog 1413
@12:17:58.94 <Air_home> Air.jog 1409
@12:17:59.05 <PinPort_home> PinPort.setRawPosition! 0
@12:17:59.07 <Archive_home> Archive.setRawPosition! 50
@12:17:59.14 <PinPort_home> PinPort.seek :home
@12:17:59.20 <Archive_home> Archive.seek :home
@12:17:59.33 <PinPort_home> PinPort.in :default
@12:17:59.51 <Archive_home> Archive.in :default
@12:17:59.86 <Plunge_home> Plunge.setRawPosition! 50
@12:17:59.88 <Lysis1_home> Lysis1.setRawPosition! 50
@12:17:59.95 <Plunge_home> Plunge.seek :home
@12:17:59.99 <Lysis1_home> Lysis1.seek :home
@12:18:00.14 <Lysis2_home> Lysis2.setRawPosition! 50
@12:18:00.15 <Air_home> Air.setRawPosition! 50
@12:18:00.22 <Lysis2_home> Lysis2.seek :home
@12:18:00.27 <Air_home> Air.seek :home
@12:18:00.42 <Plunge_home> Plunge.in :default
@12:18:00.45 <Lysis1_home> Lysis1.in :default
@12:18:00.59 <Lysis2_home> Lysis2.in :default
@12:18:00.62 <Air_home> Air.in :default
@12:18:00.73 <Toroid_home> Toroid.setRawPosition! 0
@12:18:00.79 Toroid.seek :home
@12:18:00.88 Toroid.in :default
@12:18:01.06 <LRAUV> Toroid.seek :clear
@12:18:02.68 Carousel.in :default
@12:18:02.74 Carousel.home.jog 0
@12:18:03.24 Carousel.in :default
@12:18:03.30 Carousel.seek 36
@12:18:03.44 Selecting Cartridge 35
@12:18:03.68 Carousel.seek 35
@12:18:04.17 Lysis1.in :fast
@12:18:04.23 Lysis1.seek :locked
@12:18:06.82 <Plunge> Plunge.seek :locked
@12:18:06.94 <Air> Air.in :fast
@12:18:07.00 Air.seek :locked
@12:18:09.60 <LRAUV> Powering cartridge bus
@12:18:12.23 Gate.setIR 1750,5000,0,6
@12:18:12.36 Gate.setIR 0,5000,0,6
@12:18:12.42 PuckHeater.configure :default
@12:18:12.47 Gate.power :heat,:ON,limit to 5000mA
@12:18:13.14 Mounted lysis Cartridge board#Q05042
@12:18:13.16 Using default puck heat loss curve for HeatingAccelInCarousel
@12:18:32.14 -> Cmd.startFiltering
@12:18:32.59 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@12:18:32.60 SamplePump.setPosition! 0ml
@12:18:32.65 Bypass.open
@12:18:34.15 Intake.open
@12:18:34.95 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@12:18:37.52 Vacuum tare is 51% of full scale
@12:18:37.71 Pressure.in :default
@12:18:37.77 Pressure.hold
@12:18:37.93 SamplePump.in :prime
@12:18:38.00 SamplePump.seek 10ml,59 seconds
@12:18:47.26 Pressure.stop
@12:18:47.31 SamplePump.setPosition! 0ml
@12:18:47.36 Exhaust.open
@12:18:48.81 <closeBypass> Bypass.close
@12:18:50.38 <FILTERING> Pressure.in :default
@12:18:50.44 Pressure.hold
@12:18:50.66 SamplePump.in :prime
@12:18:50.72 SamplePump.seek 100ml,5:16
@12:20:55.97 Pressure.stop
@12:20:56.03 Bypass.open
@12:20:56.77 Intake.close
@12:20:57.62 Toroid.in :hold
@12:20:57.68 Toroid.seek :crack
@12:20:59.19 Toroid.seek :cartridge
@12:21:00.27 Toroid.in :default
@12:21:00.33 Toroid.seek :clear
@12:21:02.28 Pressurizing cartridge to 14psi above 12.7psia
@12:21:02.30 SamplePump.setPosition! 0ml
@12:21:02.34 Intake.open
@12:21:03.09 Bypass.close
@12:21:03.83 Exhaust.close
@12:21:04.63 Pressure.in :default
@12:21:04.69 Pressure.hold
@12:21:04.85 SamplePump.in :charge
@12:21:04.92 SamplePump.seek 2.3ml,41 seconds
@12:21:15.64 Pressure.stop
@12:21:15.69 Intake.close
@12:21:16.54 Pressurized cartridge with 0.511ml to 14.2psi
@12:21:16.55 Isolated @23.5psia (10.8psi)
@12:21:16.56 Verifying cartridge holds at least 0.75..0.85 of 23.5psia
@12:21:16.60 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@12:21:21.17 Cartridge pressure fell 8% to 21.5psia
@12:21:21.25 Sampling 75.0ml
@12:21:21.26 SamplePump.setPosition! 0ml
@12:21:21.31 Exhaust.open
@12:21:22.05 Intake.open
@12:21:22.97 Pressure.in :default
@12:21:23.03 Pressure.hold
@12:21:23.25 SamplePump.in :sample
@12:21:23.32 SamplePump.seek 75ml,5:31
@12:21:26.00 <sampling> Cartridge 35 filtering 1/75ml at 0.34ml/s with 13.1psi vacuum
@12:22:26.01 Cartridge 35 filtering 21/75ml at 0.31ml/s with 11.2psi vacuum
@12:23:26.01 Cartridge 35 filtering 40/75ml at 0.31ml/s with 11.0psi vacuum
@12:24:26.01 Cartridge 35 filtering 59/75ml at 0.30ml/s with 11.6psi vacuum
@12:25:15.48 <FILTERING> Pressure.stop
@12:25:15.60 <closeIntake> Intake.close
@12:25:15.64 <closeExhaust> Exhaust.close
@12:25:15.80 <FILTERING> Toroid.in :hold
@12:25:15.86 Toroid.seek :bypass
@12:25:17.48 Toroid.in :default
@12:25:17.54 Toroid.seek :clear
@12:25:19.16 Sampled  75.0ml
@12:25:20.62 <LRAUV> -> Cmd.startProcessing
@12:25:22.14 <PROCESSING> SPR.command \"read_sequences\"
@12:25:22.48 SPR.command \"stats_on\"
@12:25:22.52 SPR.command \"start 0 1\"
@12:25:23.33 <SPRlogger> SPR.start /var/log/esp/spr21aug0912h.spr.bz2
@12:25:25.34 <PROCESSING> Handoff.select :waste
@12:25:32.04 Syringe tare is 19% of full scale
@12:25:32.06 PinPort.in :locking
@12:25:32.14 PinPort.seek :locked
@12:25:32.61 <posArchive> Archive.in :fast
@12:25:32.67 <posLyS2> Lysis2.in :fast
@12:25:32.72 <PROCESSING> Lysis1.seek 1350uL
@12:25:32.78 <posArchive> Archive.seek :full
@12:25:32.85 <posLyS2> Lysis2.seek :full
@12:25:34.97 <PROCESSING> Plunge.seek :reagent
@12:25:35.87 Evacuating puck with filter bubble point of 24psi
@12:25:35.89 PinPort.seek :locked
@12:25:35.97 Handoff.select :waste
@12:25:42.56 Air syringe pumping up to 1 stroke to reach 8psi
@12:25:42.57 Air stroke #1 of 1
@12:25:42.65 Air.in :pressurize
@12:25:42.71 Air.seek :pressurized
@12:25:53.97 -0.1psi after pressurize
@12:25:53.98 Air.in :compress
@12:25:54.05 Air.seek :evac
@12:25:58.13 -0.0psi after compress
@12:26:10.70 -0.1psi after compress (stable)
@12:26:10.71 Air.in :default
@12:26:10.78 Air.hold
@12:26:10.96 at -0.1psi Opened to waste
@12:26:10.97 Handoff.select :blocked
@12:26:11.11 Archive syringe pumping up to 8 strokes to reach 24psi
@12:26:11.12 Archive stroke #1 of 8
@12:26:11.21 Archive.in :pressurize
@12:26:11.29 Archive.seek :pressurized
@12:26:24.03 -0.0psi after pressurize
@12:26:24.05 Archive.in :compress
@12:26:24.12 Archive.seek :empty
@12:26:38.90 Archive.in :default
@12:26:38.96 Archive.hold
@12:26:39.03 Handoff.select :blocked
@12:26:39.04 SPR.command \"start 2 14\"
@12:26:40.05 SPR stabilized
@12:26:40.36 <SPRlogger> Ended log spr21aug0912h.spr.bz2
@12:26:40.37 <PROCESSING> SPR.command \"stats_off\"
@12:26:41.30 Archive.setRawPosition! -8241
@12:26:41.35 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 22uL (sensed 47uL)
@12:26:41.37 Thread::ChildDied in LRAUV -- :PROCESSING
@12:26:45.04 <LRAUV> -> Cmd.stop
@12:26:45.12 Gate.power :heat,:OFF
@12:26:45.14 Gate.power :cartridge,:OFF
@12:26:45.22 Carousel.seek 35
@12:26:45.40 Plunge.seek :locked
@12:26:46.28 PinPort.in :unlocking
@12:26:46.34 PinPort.seek :clear
@12:26:46.90 <Archive> Archive.in :unlocking
@12:26:47.02 Archive.seek :clear
@12:26:47.06 <Air> Air.in :unlocking
@12:26:47.22 Air.seek :clear
@12:26:47.25 <Lysis1> Lysis1.in :unlocking
@12:26:47.85 Lysis1.seek :clear
@12:26:47.92 <Lysis2> Lysis2.in :unlocking
@12:26:47.99 Lysis2.seek :clear
@12:26:51.00 Slide::Error in LRAUV -- Archive Syringe (ArS) positionErr at 993uL (sensed 969uL)
@12:26:51.02 <LRAUV> Retry #1 of 2
@12:26:51.18 Gate.power :motor,:OFF
@12:26:51.20 Gate.power :core,:OFF
@12:26:51.22 Gate.power :analytic1,:OFF
@12:26:51.24 Gate.power :analytic2,:OFF
@12:26:51.26 Gate.power :analytic3,:OFF
@12:26:51.28 Gate.power :main,:OFF
@12:26:55.03 Gate.power :main,:ON,limit to 5000mA
@12:26:55.04 Gate.power :analytic1,:ON,limit to 5000mA
@12:26:55.07 Gate.power :analytic2,:ON,limit to 5000mA
@12:26:55.09 Gate.power :analytic3,:ON,limit to 5000mA
@12:26:55.16 Powering core bus
@12:26:57.54 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@12:26:57.89 <LRAUV> Archive.configure :default
@12:26:58.02 Air.configure :default
@12:26:58.12 Lysis1.configure :default
@12:26:58.23 Lysis2.configure :default
@12:26:58.35 Plunge.configure :default
@12:26:58.47 SamplePump.configure :default
@12:26:58.62 ExtraPump.configure :default
@12:26:58.73 Toroid.configure :default
@12:26:58.85 Carousel.configure :default
@12:26:58.98 Pressure.configure :default
@12:26:59.09 PinPort.configure :default
@12:26:59.16 All elf controllers running firmware version 4.741
@12:26:59.18 Gate.power :motor,:ON,limit to 5000mA
@12:26:59.27 Archive.setRawPosition! -3661
@12:26:59.37 Air.setRawPosition! 4
@12:26:59.46 Lysis1.setRawPosition! 12
@12:26:59.56 Lysis2.setRawPosition! 22
@12:26:59.65 Plunge.setRawPosition! -1271
@12:26:59.75 Toroid.setRawPosition! -469
@12:26:59.84 Carousel.setRawPosition! 8503
@12:26:59.93 PinPort.setRawPosition! -1
@12:27:00.09 Carousel.seek 35
@12:27:00.30 Lysis1.in :fast
@12:27:00.36 Lysis1.seek :locked
@12:27:02.89 Plunge.seek :locked
@12:27:03.22 <Archive> Archive.in :unlocking
@12:27:03.34 Archive.seek :clear
@12:27:03.42 <Air> Air.in :unlocking
@12:27:03.55 Air.seek :clear
@12:27:03.64 <Lysis1> Lysis1.in :unlocking
@12:27:03.76 Lysis1.seek :clear
@12:27:03.82 <Lysis2> Lysis2.in :unlocking
@12:27:03.91 Lysis2.seek :clear
@12:27:05.57 <LRAUV> Gate.power :motor,:OFF
@12:27:05.59 Gate.power :core,:OFF
@12:27:05.61 Gate.power :analytic1,:OFF
@12:27:05.63 Gate.power :analytic2,:OFF
@12:27:05.65 Gate.power :analytic3,:OFF
@12:27:05.67 Gate.power :main,:OFF
@12:27:05.69 Safely stopped and ready to power off
##-- file closed 2021-08-09T17:17:35.610Z

##-- file opened 2021-08-09T17:17:35.612Z
@13:17:35.98 -> showStatus
@13:17:37.99 -> Cmd.loadCartridge -4
@13:17:38.05 Gate.power :main,:ON,limit to 5000mA
@13:17:38.06 Gate.power :analytic1,:ON,limit to 5000mA
@13:17:38.08 Gate.power :analytic2,:ON,limit to 5000mA
@13:17:38.10 Gate.power :analytic3,:ON,limit to 5000mA
@13:17:38.27 Powering core bus
@13:17:40.52 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@13:17:41.01 <LRAUV> Archive.configure :default
@13:17:41.13 Air.configure :default
@13:17:41.24 Lysis1.configure :default
@13:17:41.69 Lysis2.configure :default
@13:17:41.82 Plunge.configure :default
@13:17:41.94 SamplePump.configure :default
@13:17:42.08 ExtraPump.configure :default
@13:17:42.19 Toroid.configure :default
@13:17:42.31 Carousel.configure :default
@13:17:42.44 Pressure.configure :default
@13:17:42.56 PinPort.configure :default
@13:17:42.62 All elf controllers running firmware version 4.741
@13:17:42.64 Gate.power :motor,:ON,limit to 5000mA
@13:17:42.85 <Archive_home> Archive.in :default
@13:17:43.06 Archive.home.jog 0
@13:17:43.21 <Toroid_home> Toroid.in :default
@13:17:43.28 <Plunge_home> Plunge.in :default
@13:17:43.32 <Lysis1_home> Lysis1.in :default
@13:17:43.35 <Air_home> Air.in :default
@13:17:43.40 <PinPort_home> PinPort.in :default
@13:17:43.49 <Lysis2_home> Lysis2.in :default
@13:17:43.61 <Toroid_home> Toroid.home.jog 0
@13:17:43.69 <Lysis1_home> Lysis1.home.jog 0
@13:17:43.72 <PinPort_home> PinPort.home.jog 0
@13:17:43.76 <Plunge_home> Plunge.home.jog 0
@13:17:43.80 <Air_home> Air.home.jog 0
@13:17:43.83 <Lysis2_home> Lysis2.home.jog 0
@13:17:44.18 <Archive_home> Archive.in :homing
@13:17:44.38 <Toroid_home> Toroid.in :homing
@13:17:44.41 <Plunge_home> Plunge.in :homing
@13:17:44.44 <Lysis1_home> Lysis1.in :homing
@13:17:44.49 <Archive_home> Archive.jog 1367
@13:17:44.55 <PinPort_home> PinPort.in :homing
@13:17:44.74 PinPort.jog -108
@13:17:44.78 <Toroid_home> Toroid.jog 615
@13:17:44.80 <Plunge_home> Plunge.jog 1535
@13:17:44.83 <Lysis1_home> Lysis1.jog 150
@13:17:45.04 <Lysis2_home> Lysis2.in :homing
@13:17:45.07 <Air_home> Air.in :homing
@13:17:45.18 <Lysis2_home> Lysis2.jog 1428
@13:17:45.21 <PinPort_home> PinPort.setRawPosition! 0
@13:17:45.27 <Air_home> Air.jog 1412
@13:17:45.34 <PinPort_home> PinPort.seek :home
@13:17:45.45 PinPort.in :default
@13:17:45.47 <Lysis1_home> Lysis1.setRawPosition! 50
@13:17:45.55 Lysis1.seek :home
@13:17:45.81 <Archive_home> Archive.setRawPosition! 50
@13:17:45.86 Archive.seek :home
@13:17:45.94 <Lysis1_home> Lysis1.in :default
@13:17:46.08 <Plunge_home> Plunge.setRawPosition! 50
@13:17:46.14 Plunge.seek :home
@13:17:46.22 <Archive_home> Archive.in :default
@13:17:46.38 <Lysis2_home> Lysis2.setRawPosition! 50
@13:17:46.44 Lysis2.seek :home
@13:17:46.54 <Plunge_home> Plunge.in :default
@13:17:46.56 <Air_home> Air.setRawPosition! 50
@13:17:46.65 Air.seek :home
@13:17:46.76 <Lysis2_home> Lysis2.in :default
@13:17:46.94 <Air_home> Air.in :default
@13:17:47.03 <Toroid_home> Toroid.setRawPosition! 0
@13:17:47.07 Toroid.seek :home
@13:17:47.17 Toroid.in :default
@13:17:47.32 <LRAUV> Toroid.seek :clear
@13:17:48.94 Carousel.in :default
@13:17:49.00 Carousel.home.jog 0
@13:17:49.49 Carousel.in :default
@13:17:49.56 Carousel.seek 35
@13:17:49.71 Selecting Cartridge 34
@13:17:49.95 Carousel.seek 34
@13:17:50.44 Lysis1.in :fast
@13:17:50.50 Lysis1.seek :locked
@13:17:53.09 <Plunge> Plunge.seek :locked
@13:17:53.21 <Air> Air.in :fast
@13:17:53.28 Air.seek :locked
@13:17:55.88 <LRAUV> Powering cartridge bus
@13:17:58.50 Gate.setIR 1750,5000,0,6
@13:17:58.63 Gate.setIR 0,5000,0,6
@13:17:58.68 Gate.power :cartridge,:OFF
@13:17:59.21 Mounted archive Cartridge
@13:18:17.57 -> Cmd.startFiltering
@13:18:18.02 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@13:18:18.04 SamplePump.setPosition! 0ml
@13:18:18.08 Bypass.open
@13:18:19.55 Intake.open
@13:18:20.37 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@13:18:22.93 Vacuum tare is 50% of full scale
@13:18:23.06 Pressure.in :default
@13:18:23.12 Pressure.hold
@13:18:23.29 SamplePump.in :prime
@13:18:23.35 SamplePump.seek 10ml,59 seconds
@13:18:32.61 Pressure.stop
@13:18:32.66 SamplePump.setPosition! 0ml
@13:18:32.71 Exhaust.open
@13:18:34.16 <closeBypass> Bypass.close
@13:18:35.70 <FILTERING> Pressure.in :default
@13:18:35.76 Pressure.hold
@13:18:35.98 SamplePump.in :prime
@13:18:36.05 SamplePump.seek 100ml,5:16
@13:20:41.18 Pressure.stop
@13:20:41.24 Bypass.open
@13:20:41.96 Intake.close
@13:20:42.83 Toroid.in :hold
@13:20:42.89 Toroid.seek :crack
@13:20:44.40 Toroid.seek :cartridge
@13:20:45.48 Toroid.in :default
@13:20:45.55 Toroid.seek :clear
@13:20:47.51 Pressurizing cartridge to 26psi above 14.5psia
@13:20:47.52 SamplePump.setPosition! 0ml
@13:20:47.56 Intake.open
@13:20:48.34 Bypass.close
@13:20:49.07 Exhaust.close
@13:20:49.89 Pressure.in :default
@13:20:49.95 Pressure.hold
@13:20:50.12 SamplePump.in :charge
@13:20:50.19 SamplePump.seek 2.3ml,42 seconds
@13:20:57.09 Pressure.stop
@13:20:57.14 Intake.close
@13:20:57.98 Pressurized cartridge with 0.210ml to 27.8psi
@13:20:57.99 Isolated @37.3psia (22.8psi)
@13:20:58.00 Verifying cartridge holds at least 0.75..0.85 of 37.3psia
@13:20:58.03 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@13:21:02.60 Cartridge pressure fell 1% to 36.9psia
@13:21:02.68 Sampling 1000.0ml
@13:21:02.69 SamplePump.setPosition! 0ml
@13:21:02.74 Exhaust.open
@13:21:03.44 Intake.open
@13:21:04.32 Pressure.in :default
@13:21:04.38 Pressure.hold
@13:21:04.61 SamplePump.in :sample
@13:21:04.67 SamplePump.seek 1000ml,1:23:53
@13:21:07.36 <sampling> Cartridge 34 filtering 1/1000ml at 0.42ml/s with 21.3psi vacuum
@13:22:07.35 Cartridge 34 filtering 24/1000ml at 0.41ml/s with 21.3psi vacuum
@13:23:07.36 Cartridge 34 filtering 46/1000ml at 0.33ml/s with 21.6psi vacuum
@13:24:07.35 Cartridge 34 filtering 67/1000ml at 0.33ml/s with 21.2psi vacuum
@13:25:07.36 Cartridge 34 filtering 87/1000ml at 0.32ml/s with 22.0psi vacuum
@13:26:07.35 Cartridge 34 filtering 107/1000ml at 0.36ml/s with 22.4psi vacuum
@13:27:07.36 Cartridge 34 filtering 126/1000ml at 0.28ml/s with 27.3psi vacuum
@13:28:07.35 Cartridge 34 filtering 143/1000ml at 0.26ml/s with 27.4psi vacuum
@13:29:07.36 Cartridge 34 filtering 160/1000ml at 0.23ml/s with 23.2psi vacuum
@13:30:07.35 Cartridge 34 filtering 176/1000ml at 0.30ml/s with 23.1psi vacuum
@13:31:07.36 Cartridge 34 filtering 191/1000ml at 0.25ml/s with 22.7psi vacuum
@13:32:07.35 Cartridge 34 filtering 205/1000ml at 0.25ml/s with 24.0psi vacuum
@13:33:07.36 Cartridge 34 filtering 218/1000ml at 0.20ml/s with 28.1psi vacuum
@13:34:07.35 Cartridge 34 filtering 231/1000ml at 0.20ml/s with 30.8psi vacuum
@13:34:08.05 <FILTERING> Flow averaged 0.29ml/s over 13:04
@13:34:08.08 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi
@13:34:08.66 Relieved excess vacuum in .6 seconds
@13:34:08.68 SamplePump.seek 1000ml,1:04:38
@13:34:20.86 Flow averaged 0.20ml/s over 13 seconds
@13:34:20.88 <closeIntake> Intake.close
@13:34:20.93 <closeExhaust> Exhaust.close
@13:34:21.11 <FILTERING> Toroid.in :hold
@13:34:21.18 Toroid.seek :bypass
@13:34:22.81 Toroid.in :default
@13:34:22.87 Toroid.seek :clear
@13:34:24.49 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@13:34:24.50 Pressure.stop
@13:34:24.75 Sampled  233.3ml
@13:34:26.81 <LRAUV> -> Cmd.startProcessing
@13:34:27.18 <PROCESSING> Plunge.seek :reagent
@13:34:28.02 Sample loop closed. Ready to Deliver Reagents
@13:34:28.03 Archive.seek :sealed
@13:34:32.31 Archive.seek :evac
@13:34:46.24 delay 1:00
@13:35:46.26 Archive.seek :clear
@13:36:03.97 RNAlater soak for 10:00
@13:46:03.99 Archive.seek :tVent
@13:46:06.57 delay 1 second
@13:46:07.59 Archive.seek :evac
@13:46:23.21 delay 10 seconds
@13:46:33.24 Archive.seek :tVent
@13:46:48.85 delay 1 second
@13:46:49.87 Archive.seek :evac
@13:47:05.48 delay 10 seconds
@13:47:15.51 Sample preserved and evacuated
@13:47:16.53 <LRAUV> -> Cmd.stop
@13:47:16.66 Carousel.seek 34
@13:47:16.84 Plunge.seek :locked
@13:47:17.78 <Archive> Archive.in :unlocking
@13:47:17.91 Archive.seek :clear
@13:47:17.94 <Air> Air.in :unlocking
@13:47:18.10 Air.seek :clear
@13:47:18.18 <Lysis1> Lysis1.in :unlocking
@13:47:18.29 Lysis1.seek :clear
@13:47:22.16 <LRAUV> Gate.power :motor,:OFF
@13:47:22.18 Gate.power :core,:OFF
@13:47:22.20 Gate.power :analytic1,:OFF
@13:47:22.22 Gate.power :analytic2,:OFF
@13:47:22.25 Gate.power :analytic3,:OFF
@13:47:22.27 Gate.power :main,:OFF
@13:47:22.29 Safely stopped and ready to power off
##-- file closed 2021-08-09T17:59:01.916Z

##-- file opened 2021-08-09T17:59:01.917Z
@13:59:02.68 -> showStatus
@13:59:04.71 -> Cmd.loadCartridge -18
@13:59:04.77 Gate.power :main,:ON,limit to 5000mA
@13:59:04.79 Gate.power :analytic1,:ON,limit to 5000mA
@13:59:04.81 Gate.power :analytic2,:ON,limit to 5000mA
@13:59:04.83 Gate.power :analytic3,:ON,limit to 5000mA
@13:59:04.97 Powering core bus
@13:59:07.25 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@13:59:07.72 <LRAUV> Archive.configure :default
@13:59:07.84 Air.configure :default
@13:59:08.29 Lysis1.configure :default
@13:59:08.39 Lysis2.configure :default
@13:59:08.51 Plunge.configure :default
@13:59:08.64 SamplePump.configure :default
@13:59:08.78 ExtraPump.configure :default
@13:59:08.90 Toroid.configure :default
@13:59:09.02 Carousel.configure :default
@13:59:09.15 Pressure.configure :default
@13:59:09.26 PinPort.configure :default
@13:59:09.33 All elf controllers running firmware version 4.741
@13:59:09.36 Gate.power :motor,:ON,limit to 5000mA
@13:59:09.53 <Archive_home> Archive.in :default
@13:59:09.82 Archive.home.jog 0
@13:59:09.99 <Toroid_home> Toroid.in :default
@13:59:10.06 <Plunge_home> Plunge.in :default
@13:59:10.09 <Lysis1_home> Lysis1.in :default
@13:59:10.13 <PinPort_home> PinPort.in :default
@13:59:10.18 <Lysis2_home> Lysis2.in :default
@13:59:10.21 <Air_home> Air.in :default
@13:59:10.41 <Toroid_home> Toroid.home.jog 0
@13:59:10.44 <PinPort_home> PinPort.home.jog 0
@13:59:10.47 <Plunge_home> Plunge.home.jog 0
@13:59:10.49 <Lysis1_home> Lysis1.home.jog 0
@13:59:10.52 <Lysis2_home> Lysis2.home.jog 0
@13:59:10.56 <Air_home> Air.home.jog 0
@13:59:11.08 <PinPort_home> PinPort.in :homing
@13:59:11.13 <Toroid_home> Toroid.in :homing
@13:59:11.22 <Lysis1_home> Lysis1.in :homing
@13:59:11.27 <Archive_home> Archive.in :homing
@13:59:11.30 <Plunge_home> Plunge.in :homing
@13:59:11.47 <PinPort_home> PinPort.jog -108
@13:59:11.49 <Toroid_home> Toroid.jog 610
@13:59:11.52 <Lysis1_home> Lysis1.jog 1411
@13:59:11.55 <Archive_home> Archive.jog 158
@13:59:11.58 <Plunge_home> Plunge.jog 1566
@13:59:11.81 <Air_home> Air.in :homing
@13:59:11.84 <Lysis2_home> Lysis2.in :homing
@13:59:11.93 Lysis2.jog 1414
@13:59:11.96 <Air_home> Air.jog 1407
@13:59:12.06 <PinPort_home> PinPort.setRawPosition! 0
@13:59:12.12 PinPort.seek :home
@13:59:12.20 PinPort.in :default
@13:59:12.23 <Archive_home> Archive.setRawPosition! 50
@13:59:12.32 Archive.seek :home
@13:59:12.60 Archive.in :default
@13:59:12.79 <Lysis1_home> Lysis1.setRawPosition! 50
@13:59:12.83 Lysis1.seek :home
@13:59:12.92 <Plunge_home> Plunge.setRawPosition! 50
@13:59:12.96 Plunge.seek :home
@13:59:13.12 <Lysis1_home> Lysis1.in :default
@13:59:13.20 <Lysis2_home> Lysis2.setRawPosition! 50
@13:59:13.24 <Air_home> Air.setRawPosition! 50
@13:59:13.30 <Lysis2_home> Lysis2.seek :home
@13:59:13.34 <Air_home> Air.seek :home
@13:59:13.48 <Plunge_home> Plunge.in :default
@13:59:13.63 <Lysis2_home> Lysis2.in :default
@13:59:13.74 <Toroid_home> Toroid.setRawPosition! 0
@13:59:13.76 <Air_home> Air.in :default
@13:59:13.82 <Toroid_home> Toroid.seek :home
@13:59:13.93 Toroid.in :default
@13:59:14.20 <LRAUV> Toroid.seek :clear
@13:59:15.81 Carousel.in :default
@13:59:15.87 Carousel.home.jog 0
@13:59:16.36 Carousel.in :default
@13:59:16.43 Carousel.seek 34
@13:59:16.59 Selecting Cartridge 33
@13:59:16.84 Carousel.seek 33
@13:59:17.32 Lysis1.in :fast
@13:59:17.38 Lysis1.seek :locked
@13:59:19.97 <Plunge> Plunge.seek :locked
@13:59:20.09 <Air> Air.in :fast
@13:59:20.15 Air.seek :locked
@13:59:22.75 <LRAUV> Powering cartridge bus
@13:59:25.38 Gate.setIR 1750,5000,0,6
@13:59:25.51 Gate.setIR 0,5000,0,6
@13:59:25.57 PuckHeater.configure :default
@13:59:25.62 Gate.power :heat,:ON,limit to 5000mA
@13:59:26.29 Mounted lysis Cartridge board#Q05087
@13:59:26.32 Using default puck heat loss curve for HeatingAccelInCarousel
@13:59:45.11 -> Cmd.startFiltering
@13:59:45.56 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@13:59:45.58 SamplePump.setPosition! 0ml
@13:59:45.63 Bypass.open
@13:59:47.12 Intake.open
@13:59:47.95 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@13:59:50.52 Vacuum tare is 51% of full scale
@13:59:50.65 Pressure.in :default
@13:59:50.71 Pressure.hold
@13:59:50.87 SamplePump.in :prime
@13:59:50.94 SamplePump.seek 10ml,59 seconds
@14:00:00.20 Pressure.stop
@14:00:00.25 SamplePump.setPosition! 0ml
@14:00:00.30 Exhaust.open
@14:00:01.74 <closeBypass> Bypass.close
@14:00:03.28 <FILTERING> Pressure.in :default
@14:00:03.34 Pressure.hold
@14:00:03.55 SamplePump.in :prime
@14:00:03.62 SamplePump.seek 100ml,5:16
@14:02:08.71 Pressure.stop
@14:02:08.77 Bypass.open
@14:02:09.49 Intake.close
@14:02:10.37 Toroid.in :hold
@14:02:10.43 Toroid.seek :crack
@14:02:11.94 Toroid.seek :cartridge
@14:02:13.03 Toroid.in :default
@14:02:13.09 Toroid.seek :clear
@14:02:15.05 Pressurizing cartridge to 14psi above 12.7psia
@14:02:15.06 SamplePump.setPosition! 0ml
@14:02:15.10 Intake.open
@14:02:15.38 Bypass.close
@14:02:16.12 Exhaust.close
@14:02:16.93 Pressure.in :default
@14:02:16.99 Pressure.hold
@14:02:17.16 SamplePump.in :charge
@14:02:17.23 SamplePump.seek 2.3ml,41 seconds
@14:02:27.42 Pressure.stop
@14:02:27.88 Intake.close
@14:02:28.71 Pressurized cartridge with 0.464ml to 14.3psi
@14:02:28.72 Isolated @22.5psia (9.8psi)
@14:02:28.73 Verifying cartridge holds at least 0.75..0.85 of 22.5psia
@14:02:28.76 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@14:02:33.33 Cartridge pressure fell 5% to 21.4psia
@14:02:33.41 Sampling 75.0ml
@14:02:33.42 SamplePump.setPosition! 0ml
@14:02:33.47 Exhaust.open
@14:02:34.19 Intake.open
@14:02:35.06 Pressure.in :default
@14:02:35.12 Pressure.hold
@14:02:35.34 SamplePump.in :sample
@14:02:35.41 SamplePump.seek 75ml,5:31
@14:02:38.09 <sampling> Cartridge 33 filtering 1/75ml at 0.34ml/s with 10.6psi vacuum
@14:03:38.09 Cartridge 33 filtering 21/75ml at 0.35ml/s with 14.9psi vacuum
@14:04:38.09 Cartridge 33 filtering 41/75ml at 0.31ml/s with 11.8psi vacuum
@14:05:38.09 Cartridge 33 filtering 60/75ml at 0.32ml/s with 12.5psi vacuum
@14:06:23.67 <FILTERING> Pressure.stop
@14:06:23.79 <closeIntake> Intake.close
@14:06:23.84 <closeExhaust> Exhaust.close
@14:06:23.99 <FILTERING> Toroid.in :hold
@14:06:24.06 Toroid.seek :bypass
@14:06:25.67 Toroid.in :default
@14:06:25.73 Toroid.seek :clear
@14:06:27.35 Sampled  75.0ml
@14:06:28.72 <LRAUV> -> Cmd.startProcessing
@14:06:30.25 <PROCESSING> SPR.command \"read_sequences\"
@14:06:30.73 SPR.command \"stats_on\"
@14:06:30.77 SPR.command \"start 0 1\"
@14:06:31.58 <SPRlogger> SPR.start /var/log/esp/spr21aug0914h.spr.bz2
@14:06:34.09 <PROCESSING> Handoff.select :waste
@14:06:40.79 Syringe tare is 19% of full scale
@14:06:40.81 PinPort.in :locking
@14:06:40.88 PinPort.seek :locked
@14:06:41.36 <posArchive> Archive.in :fast
@14:06:41.41 <posLyS2> Lysis2.in :fast
@14:06:41.51 <PROCESSING> Lysis1.seek 1350uL
@14:06:41.56 <posArchive> Archive.seek :full
@14:06:41.68 <posLyS2> Lysis2.seek :full
@14:06:43.78 <PROCESSING> Plunge.seek :reagent
@14:06:44.69 Evacuating puck with filter bubble point of 24psi
@14:06:44.71 PinPort.seek :locked
@14:06:44.79 Handoff.select :waste
@14:06:51.37 Air syringe pumping up to 1 stroke to reach 8psi
@14:06:51.38 Air stroke #1 of 1
@14:06:51.46 Air.in :pressurize
@14:06:51.52 Air.seek :pressurized
@14:07:02.77 1.6psi after pressurize
@14:07:02.78 Air.in :compress
@14:07:02.86 Air.seek :evac
@14:07:06.95 0.6psi after compress
@14:07:22.52 0.4psi after compress (stable)
@14:07:22.53 Air.in :default
@14:07:22.60 Air.hold
@14:07:22.77 at 0.3psi Opened to waste
@14:07:22.78 Handoff.select :blocked
@14:07:22.92 Archive syringe pumping up to 8 strokes to reach 24psi
@14:07:22.93 Archive stroke #1 of 8
@14:07:23.01 Archive.in :pressurize
@14:07:23.07 Archive.seek :pressurized
@14:07:35.80 0.7psi after pressurize
@14:07:35.82 Archive.in :compress
@14:07:35.89 Archive.seek :empty
@14:07:51.77 9.9psi after compress
@14:08:06.35 16.3psi after compress (stable)
@14:08:06.36 Archive.in :gulp
@14:08:06.43 Archive.seek :topVent
@14:08:13.64 16.5psi after gulp
@14:08:26.21 16.5psi after gulp (stable)
@14:08:26.22 Archive stroke #2 of 8
@14:08:26.31 Archive.in :pressurize
@14:08:26.37 Archive.seek :pressurized
@14:08:39.10 16.5psi after pressurize
@14:08:39.11 Archive.in :compress
@14:08:39.19 Archive.seek :empty
@14:08:50.92 Reached 24.2 of 24.0psi after pumping 3372uL (1.95 strokes)
@14:08:50.93 Archive.in :default
@14:08:51.00 Archive.hold
@14:08:51.07 Handoff.select :waste
@14:09:01.27 delay 1:00
@14:10:01.29 Handoff.select :blocked
@14:10:23.96 Handoff.select :waste
@14:10:24.08 0.5psi at evacEnd
@14:10:24.09 Handoff.select :blocked
@14:10:24.22 Archive.in :fast
@14:10:24.28 Archive.seek :topVent
@14:10:37.70 Stable @0.6psi after Handoff blocked
@14:10:37.72 Archive syringe pumping up to 8 strokes to reach 16psi
@14:10:37.74 Archive stroke #1 of 8
@14:10:37.83 Archive.in :pressurize
@14:10:37.89 Archive.seek :pressurized
@14:10:50.62 0.7psi after pressurize
@14:10:50.64 Archive.in :compress
@14:10:50.71 Archive.seek :empty
@14:11:06.58 14.8psi after compress
@14:11:19.15 15.3psi after compress (stable)
@14:11:19.17 Archive.in :gulp
@14:11:19.24 Archive.seek :topVent
@14:11:26.44 15.3psi after gulp
@14:11:39.01 15.3psi after gulp (stable)
@14:11:39.02 Archive stroke #2 of 8
@14:11:39.12 Archive.in :pressurize
@14:11:39.18 Archive.seek :pressurized
@14:11:51.93 15.2psi after pressurize
@14:11:51.95 Archive.in :compress
@14:11:52.02 Archive.seek :empty
@14:11:57.40 Reached 15.7 of 15.6psi after pumping 3235uL (1.87 strokes)
@14:11:57.41 Archive.in :default
@14:11:57.48 Archive.hold
@14:11:57.56 Handoff.select :waste
@14:12:04.75 delay 1:00
@14:13:04.77 Handoff.select :blocked
@14:13:27.46 Handoff.select :waste
@14:13:27.57 0.5psi at evacEnd
@14:13:27.59 Air.seek :fullVent
@14:13:29.04 Archive.in :fast
@14:13:29.10 Archive.seek :topVent
@14:13:35.45 Seawater evacuated, Presure above/below filter released. Lysis begins
@14:13:35.54 Lysis1.in :slow
@14:13:35.60 Lysis1.seek 1050uL
@14:14:05.61 delay 5 seconds
@14:14:10.64 Handoff.select :blocked
@14:14:10.80 Lysis1.seek 450uL
@14:15:10.76 delay 3 seconds
@14:15:13.83 Air.in to mix into 1.37806 parts Lysis1
@14:15:14.43 <Airmixer> Air.seek :evac
@14:15:14.48 <PROCESSING> Lysis1.seek 300uL
@14:15:29.56 Lysis1.seek :empty
@14:15:59.65 Lysis buffer delivered @14.0psi
@14:15:59.67 Polling Can every 2:00
@14:16:00.23 PuckHeater.configure :default #ambient 25.1C
@14:16:00.28 PuckHeater.seek 79.6C
@14:16:47.69 PuckHeater.hold 2:00,79.6C
@14:18:47.85 Polling Can every 2:00
@14:18:48.39 PuckHeater.configure :default #ambient 25.1C
@14:18:48.44 PuckHeater.hold 30 seconds,78.6C
@14:19:18.64 Polling Can every 2:00
@14:19:19.19 PuckHeater.hold 30 seconds,78.1C
@14:19:49.40 Polling Can every 2:00
@14:19:49.95 PuckHeater.configure :default #ambient 25.1C
@14:19:50.00 PuckHeater.hold 10:00,77.5C
@14:29:50.30 Lysis complete. Syringe pressure @13.3psi
@14:29:50.31 Handoff.select :blocked
@14:29:50.36 Archive syringe pumping up to 5 strokes to reach 40psi
@14:29:50.37 Archive stroke #1 of 5
@14:29:50.46 Archive.in :pressurize
@14:29:50.52 Archive.seek :pressurized
@14:30:03.24 13.3psi after pressurize
@14:30:03.25 Archive.in :compress
@14:30:03.33 Archive.seek :empty
@14:30:12.39 Reached 40.6 of 40.0psi after pumping 1584uL (0.92 strokes)
@14:30:12.40 Archive.in :default
@14:30:12.46 Archive.hold
@14:30:12.52 delay 2 seconds
@14:30:14.54 Archive.in :fast
@14:30:14.61 Archive.seek :full
@14:30:21.31 Lysis1.seek 138uL
@14:30:35.20 delay 1 second
@14:30:36.23 <fillArchive> Archive.seek :full
@14:30:36.27 <PROCESSING> Lysis1.seek :empty
@14:30:50.23 Initial air pressure @50.0psi
@14:30:50.30 Lysis1.in :extract
@14:30:50.38 Lysis1.seek 600uL,nil
@14:30:54.84 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@14:30:54.85 Archive stroke #1 of 6
@14:30:54.93 Archive.in :pressurize
@14:30:55.00 Archive.seek :pressurized
@14:31:07.78 15.7psi after pressurize
@14:31:07.80 Archive.in :compress
@14:31:07.88 Archive.seek :empty
@14:31:18.20 Syringe::LysatePlungerStuck in PROCESSING -- at < 494uL during Lysis Syringe #1 (LyS1) move
@14:31:18.23 <PROCESSING> Lysis1.in :default
@14:31:18.30 Lysis1.seek 459uL
@14:31:19.01 <pump> Reached 29.3 of 25.0psi after pumping 1629uL (0.94 strokes)
@14:31:19.03 Archive.in :default
@14:31:19.12 Archive.hold
@14:31:20.12 <PROCESSING> Lysis1.in :extract
@14:31:20.19 Lysis1.seek 600uL,nil
@14:31:22.95 Initial 600uL lysate @21.3psi
@14:31:22.96 Handoff.select :blocked
@14:31:23.05 Initial air pressure @23.5psi
@14:31:23.12 Lysis1.seek 825uL,nil
@14:31:26.56 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@14:31:26.57 Archive stroke #1 of 6
@14:31:26.67 Archive.in :compress
@14:31:26.74 Archive.seek :empty
@14:31:27.39 Reached 17.3 of 17.0psi after pumping 1638uL (0.95 strokes)
@14:31:27.40 Archive.in :default
@14:31:27.48 Archive.hold
@14:31:27.75 <PROCESSING> Recovered 825uL lysate @16.2psi
@14:31:27.76 Air.in :halfSpeed
@14:31:27.83 Air.seek :fullVent
@14:31:30.08 Puck Top Vented, bottom @3.0psi
@14:31:30.09 Handoff.select :waste
@14:31:30.21 delay 5 seconds
@14:31:35.27 Handoff open to waste, bottom @0.1psi
@14:31:35.29 Air.in :default
@14:31:35.35 Air.seek :evac
@14:31:36.79 Lysis2.in :DAmix
@14:31:36.89 Lysis1.in :DAmix
@14:31:37.44 <Lysis2mixer> Lysis2.seek 1480uL
@14:31:37.48 <PROCESSING> Lysis1.seek 575uL
@14:32:12.53 delay 5 seconds
@14:32:17.55 Handoff.select SPR
@14:32:17.68 SPR.command \"start 2 14\"
@14:32:19.10 SPR stabilized
@14:32:19.23 delay 10 seconds
@14:32:29.25 SPR.command \"start 2 0\"
@14:32:32.46 Waiting up to 11:00 for SPR to enter state 1
@14:34:30.13 SPR.command \"hold_on\"
@14:34:31.52 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2
@14:34:31.96 <Lysis2mixer> Lysis2.seek 1180uL
@14:34:32.04 <PROCESSING> Lysis1.seek 275uL
@14:35:14.02 delay 15 seconds
@14:35:29.04 SPR.command \"hold_off\"
@14:35:32.69 Waiting up to 38:00 for SPR to enter state 7
@14:59:30.27 SPR analysis completed
@14:59:34.42 <SPRlogger> Ended log spr21aug0914h.spr.bz2
@14:59:34.43 <PROCESSING> SPR.command \"stats_off\"
@14:59:36.00 <LRAUV> -> Cmd.stop
@14:59:36.09 <closeHandoff> Handoff.select :close
@14:59:36.22 <LRAUV> Gate.power :heat,:OFF
@14:59:36.24 Gate.power :cartridge,:OFF
@14:59:36.32 Carousel.seek 33
@14:59:36.50 Plunge.seek :locked
@14:59:37.38 PinPort.in :unlocking
@14:59:37.45 PinPort.seek :clear
@14:59:37.98 <Archive> Archive.in :unlocking
@14:59:38.11 Archive.seek :clear
@14:59:38.15 <Air> Air.in :unlocking
@14:59:38.31 <Lysis1> Lysis1.in :unlocking
@14:59:38.35 <Air> Air.seek :clear
@14:59:38.50 <Lysis1> Lysis1.seek :clear
@14:59:38.59 <Lysis2> Lysis2.in :unlocking
@14:59:38.66 Lysis2.seek :clear
@14:59:42.26 <LRAUV> Gate.power :motor,:OFF
@14:59:42.28 Gate.power :core,:OFF
@14:59:42.30 Gate.power :analytic1,:OFF
@14:59:42.32 Gate.power :analytic2,:OFF
@14:59:42.34 Gate.power :analytic3,:OFF
@14:59:42.36 Gate.power :main,:OFF
@14:59:42.39 Safely stopped and ready to power off
##-- file closed 2021-08-10T13:01:01.640Z