##-- file opened 2021-08-07T17:09:18.985Z @13:09:19.35 -> showStatus @13:09:21.38 -> Cmd.loadCartridge -4 @13:09:21.44 Gate.power :main,:ON,limit to 5000mA @13:09:21.46 Gate.power :analytic1,:ON,limit to 5000mA @13:09:21.48 Gate.power :analytic2,:ON,limit to 5000mA @13:09:21.50 Gate.power :analytic3,:ON,limit to 5000mA @13:09:21.64 Powering core bus @13:09:23.92 SPR Module 1, FW Rev. B, FPGA Rev. C @13:09:24.38 Archive.configure :default @13:09:24.50 Air.configure :default @13:09:24.61 Lysis1.configure :default @13:09:24.71 Lysis2.configure :default @13:09:24.84 Plunge.configure :default @13:09:24.96 SamplePump.configure :default @13:09:25.11 ExtraPump.configure :default @13:09:25.21 Toroid.configure :default @13:09:25.34 Carousel.configure :default @13:09:25.47 Pressure.configure :default @13:09:25.59 PinPort.configure :default @13:09:25.65 All elf controllers running firmware version 4.741 @13:09:25.68 Gate.power :motor,:ON,limit to 5000mA @13:09:26.25 Archive.in :default @13:09:26.38 Air.in :default @13:09:26.58 Archive.home.jog 0 @13:09:26.61 Air.home.jog 0 @13:09:26.79 Toroid.in :default @13:09:26.87 Plunge.in :default @13:09:26.90 Lysis1.in :default @13:09:27.00 PinPort.in :default @13:09:27.05 Lysis2.in :default @13:09:27.22 Toroid.home.jog 0 @13:09:27.25 Plunge.home.jog 0 @13:09:27.28 PinPort.home.jog 0 @13:09:27.30 Lysis1.home.jog 0 @13:09:27.36 Lysis2.home.jog 0 @13:09:27.70 Archive.in :homing @13:09:27.75 Air.in :homing @13:09:27.90 Archive.jog 1399 @13:09:27.93 Air.jog 1412 @13:09:28.13 Toroid.in :homing @13:09:28.15 Plunge.in :homing @13:09:28.18 PinPort.in :homing @13:09:28.22 Lysis2.in :homing @13:09:28.26 Lysis1.in :homing @13:09:28.42 Plunge.jog 1566 @13:09:28.46 PinPort.jog -108 @13:09:28.49 Toroid.jog 629 @13:09:28.54 Lysis2.jog 1410 @13:09:28.58 Lysis1.jog 1415 @13:09:28.85 PinPort.setRawPosition! 0 @13:09:28.90 PinPort.seek :home @13:09:28.99 PinPort.in :default @13:09:29.09 Archive.setRawPosition! 50 @13:09:29.15 Archive.seek :home @13:09:29.23 Air.setRawPosition! 50 @13:09:29.28 Air.seek :home @13:09:29.42 Archive.in :default @13:09:29.55 Air.in :default @13:09:29.74 Plunge.setRawPosition! 50 @13:09:29.78 Plunge.seek :home @13:09:29.90 Lysis1.setRawPosition! 50 @13:09:29.92 Lysis2.setRawPosition! 50 @13:09:29.98 Lysis1.seek :home @13:09:30.02 Lysis2.seek :home @13:09:30.18 Plunge.in :default @13:09:30.34 Lysis1.in :default @13:09:30.42 Lysis2.in :default @13:09:30.72 Toroid.setRawPosition! 0 @13:09:30.77 Toroid.seek :home @13:09:30.86 Toroid.in :default @13:09:30.98 Toroid.seek :clear @13:09:32.60 Carousel.in :default @13:09:32.66 Carousel.home.jog 0 @13:09:33.15 Carousel.in :default @13:09:33.21 Carousel.seek 45 @13:09:33.39 Selecting Cartridge 44 @13:09:33.63 Carousel.seek 44 @13:09:34.13 Lysis1.in :fast @13:09:34.19 Lysis1.seek :locked @13:09:36.79 Plunge.seek :locked @13:09:36.91 Air.in :fast @13:09:36.98 Air.seek :locked @13:09:39.57 Powering cartridge bus @13:09:42.19 Gate.setIR 1750,5000,0,6 @13:09:42.32 Gate.setIR 0,5000,0,6 @13:09:42.37 Gate.power :cartridge,:OFF @13:09:42.91 Mounted archive Cartridge @13:10:00.97 -> Cmd.startFiltering @13:10:01.43 Priming sample loop w/100ml, bypass w/10ml @13:10:01.44 SamplePump.setPosition! 0ml @13:10:01.48 Bypass.open @13:10:02.99 Intake.open @13:10:03.78 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:10:06.35 Vacuum tare is 50% of full scale @13:10:06.48 Pressure.in :default @13:10:06.54 Pressure.hold @13:10:06.71 SamplePump.in :prime @13:10:06.77 SamplePump.seek 10ml,59 seconds @13:10:16.03 Pressure.stop @13:10:16.08 SamplePump.setPosition! 0ml @13:10:16.13 Exhaust.open @13:10:17.61 Bypass.close @13:10:19.17 Pressure.in :default @13:10:19.23 Pressure.hold @13:10:19.45 SamplePump.in :prime @13:10:19.52 SamplePump.seek 100ml,5:16 @13:12:23.37 Pressure.stop @13:12:23.43 Bypass.open @13:12:24.15 Intake.close @13:12:24.97 Toroid.in :hold @13:12:25.03 Toroid.seek :crack @13:12:26.54 Toroid.seek :cartridge @13:12:27.62 Toroid.in :default @13:12:27.68 Toroid.seek :clear @13:12:29.64 Pressurizing cartridge to 26psi above 14.7psia @13:12:29.65 SamplePump.setPosition! 0ml @13:12:29.69 Intake.open @13:12:29.94 Bypass.close @13:12:30.66 Exhaust.close @13:12:31.48 Pressure.in :default @13:12:31.54 Pressure.hold @13:12:31.71 SamplePump.in :charge @13:12:31.77 SamplePump.seek 2.3ml,42 seconds @13:12:38.22 Pressure.stop @13:12:38.28 Intake.close @13:12:38.75 Pressurized cartridge with 0.184ml to 26.3psi @13:12:38.77 Isolated @35.5psia (20.8psi) @13:12:38.78 Verifying cartridge holds at least 0.75..0.85 of 35.5psia @13:12:38.82 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:12:43.40 Cartridge pressure fell 1% to 35.0psia @13:12:43.47 Sampling 1000.0ml @13:12:43.49 SamplePump.setPosition! 0ml @13:12:43.54 Exhaust.open @13:12:44.23 Intake.open @13:12:44.59 Pressure.in :default @13:12:44.65 Pressure.hold @13:12:45.27 SamplePump.in :sample @13:12:45.35 SamplePump.seek 1000ml,1:23:53 @13:12:47.63 Cartridge 44 filtering 1/1000ml at 0.35ml/s with 26.6psi vacuum @13:13:47.62 Cartridge 44 filtering 23/1000ml at 0.36ml/s with 20.1psi vacuum @13:14:47.63 Cartridge 44 filtering 45/1000ml at 0.40ml/s with 25.1psi vacuum @13:15:47.62 Cartridge 44 filtering 67/1000ml at 0.36ml/s with 30.4psi vacuum @13:16:47.63 Cartridge 44 filtering 87/1000ml at 0.33ml/s with 22.7psi vacuum @13:17:47.62 Cartridge 44 filtering 107/1000ml at 0.30ml/s with 21.8psi vacuum @13:18:47.63 Cartridge 44 filtering 126/1000ml at 0.29ml/s with 22.2psi vacuum @13:19:47.62 Cartridge 44 filtering 144/1000ml at 0.25ml/s with 24.1psi vacuum @13:20:47.63 Cartridge 44 filtering 161/1000ml at 0.28ml/s with 20.9psi vacuum @13:21:47.63 Cartridge 44 filtering 177/1000ml at 0.30ml/s with 23.2psi vacuum @13:22:47.63 Cartridge 44 filtering 192/1000ml at 0.28ml/s with 26.2psi vacuum @13:23:47.63 Cartridge 44 filtering 207/1000ml at 0.24ml/s with 22.9psi vacuum @13:24:47.63 Cartridge 44 filtering 220/1000ml at 0.20ml/s with 25.9psi vacuum @13:25:47.63 Cartridge 44 filtering 234/1000ml at 0.23ml/s with 21.0psi vacuum @13:26:27.89 Flow averaged 0.29ml/s over 13:44 @13:26:27.92 Waiting up to 19 seconds for vacuum to reduce from 30.5 to 18.0psi @13:26:28.50 Relieved excess vacuum in .6 seconds @13:26:28.52 SamplePump.seek 1000ml,1:03:42 @13:26:39.50 Flow averaged 0.20ml/s over 11 seconds @13:26:39.52 Intake.close @13:26:39.56 Exhaust.close @13:26:39.75 Toroid.in :hold @13:26:39.81 Toroid.seek :bypass @13:26:41.44 Toroid.in :default @13:26:41.50 Toroid.seek :clear @13:26:43.12 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @13:26:43.13 Pressure.stop @13:26:43.32 Sampled 244.2ml @13:26:45.36 -> Cmd.startProcessing @13:26:45.73 Plunge.seek :reagent @13:26:46.57 Sample loop closed. Ready to Deliver Reagents @13:26:46.58 Archive.seek :sealed @13:26:50.88 Archive.seek :evac @13:27:04.80 delay 1:00 @13:28:04.82 Archive.seek :clear @13:28:22.52 RNAlater soak for 10:00 @13:38:22.55 Archive.seek :tVent @13:38:25.13 delay 1 second @13:38:26.15 Archive.seek :evac @13:38:41.76 delay 10 seconds @13:38:51.79 Archive.seek :tVent @13:39:07.40 delay 1 second @13:39:08.42 Archive.seek :evac @13:39:24.02 delay 10 seconds @13:39:34.05 Sample preserved and evacuated @13:39:35.00 -> Cmd.stop @13:39:35.14 Carousel.seek 44 @13:39:35.31 Plunge.seek :locked @13:39:36.25 Archive.in :unlocking @13:39:36.37 Archive.seek :clear @13:39:36.43 Air.in :unlocking @13:39:36.56 Air.seek :clear @13:39:36.62 Lysis1.in :unlocking @13:39:36.76 Lysis1.seek :clear @13:39:40.55 Gate.power :motor,:OFF @13:39:40.57 Gate.power :core,:OFF @13:39:40.59 Gate.power :analytic1,:OFF @13:39:40.61 Gate.power :analytic2,:OFF @13:39:40.63 Gate.power :analytic3,:OFF @13:39:40.66 Gate.power :main,:OFF @13:39:40.68 Safely stopped and ready to power off ##-- file closed 2021-08-07T17:48:37.970Z ##-- file opened 2021-08-07T17:48:37.972Z @13:48:38.57 -> showStatus @13:48:40.59 -> Cmd.loadCartridge -18 @13:48:40.65 Gate.power :main,:ON,limit to 5000mA @13:48:40.67 Gate.power :analytic1,:ON,limit to 5000mA @13:48:40.69 Gate.power :analytic2,:ON,limit to 5000mA @13:48:40.71 Gate.power :analytic3,:ON,limit to 5000mA @13:48:40.85 Powering core bus @13:48:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @13:48:43.59 Archive.configure :default @13:48:43.71 Air.configure :default @13:48:43.81 Lysis1.configure :default @13:48:43.92 Lysis2.configure :default @13:48:44.04 Plunge.configure :default @13:48:44.17 SamplePump.configure :default @13:48:44.31 ExtraPump.configure :default @13:48:44.42 Toroid.configure :default @13:48:44.55 Carousel.configure :default @13:48:44.67 Pressure.configure :default @13:48:44.78 PinPort.configure :default @13:48:44.85 All elf controllers running firmware version 4.741 @13:48:44.88 Gate.power :motor,:ON,limit to 5000mA @13:48:45.41 Archive.in :default @13:48:45.68 Archive.home.jog 0 @13:48:45.84 Toroid.in :default @13:48:45.92 Plunge.in :default @13:48:45.95 Lysis2.in :default @13:48:45.99 Air.in :default @13:48:46.02 PinPort.in :default @13:48:46.08 Lysis1.in :default @13:48:46.28 Toroid.home.jog 0 @13:48:46.31 Plunge.home.jog 0 @13:48:46.35 Lysis2.home.jog 0 @13:48:46.37 PinPort.home.jog 0 @13:48:46.41 Lysis1.home.jog 0 @13:48:46.45 Air.home.jog 0 @13:48:46.87 Archive.in :homing @13:48:47.09 Toroid.in :homing @13:48:47.13 Plunge.in :homing @13:48:47.16 Air.in :homing @13:48:47.20 Archive.jog 1401 @13:48:47.23 PinPort.in :homing @13:48:47.26 Lysis2.in :homing @13:48:47.30 Lysis1.in :homing @13:48:47.49 Plunge.jog 1569 @13:48:47.53 Toroid.jog 633 @13:48:47.56 PinPort.jog -109 @13:48:47.59 Air.jog 1405 @13:48:47.63 Lysis1.jog 1415 @13:48:47.68 Lysis2.jog 1410 @13:48:47.96 PinPort.setRawPosition! 0 @13:48:48.00 PinPort.seek :home @13:48:48.10 PinPort.in :default @13:48:48.53 Archive.setRawPosition! 50 @13:48:48.57 Archive.seek :home @13:48:48.83 Plunge.setRawPosition! 50 @13:48:48.88 Plunge.seek :home @13:48:49.01 Lysis2.setRawPosition! 50 @13:48:49.03 Lysis1.setRawPosition! 50 @13:48:49.06 Archive.in :default @13:48:49.09 Air.setRawPosition! 50 @13:48:49.20 Lysis2.seek :home @13:48:49.25 Lysis1.seek :home @13:48:49.38 Air.seek :home @13:48:49.49 Plunge.in :default @13:48:49.58 Lysis2.in :default @13:48:49.68 Lysis1.in :default @13:48:49.75 Air.in :default @13:48:49.84 Toroid.setRawPosition! 0 @13:48:49.89 Toroid.seek :home @13:48:50.00 Toroid.in :default @13:48:50.16 Toroid.seek :clear @13:48:51.78 Carousel.in :default @13:48:51.84 Carousel.home.jog 0 @13:48:52.34 Carousel.in :default @13:48:52.40 Carousel.seek 44 @13:48:52.56 Selecting Cartridge 43 @13:48:52.80 Carousel.seek 43 @13:48:53.30 Lysis1.in :fast @13:48:53.36 Lysis1.seek :locked @13:48:55.95 Plunge.seek :locked @13:48:56.07 Air.in :fast @13:48:56.13 Air.seek :locked @13:48:58.74 Powering cartridge bus @13:49:01.36 Gate.setIR 1750,5000,0,6 @13:49:01.49 Gate.setIR 0,5000,0,6 @13:49:01.55 PuckHeater.configure :default @13:49:01.60 Gate.power :heat,:ON,limit to 5000mA @13:49:02.28 Mounted lysis Cartridge board#Q05078 @13:49:02.32 Ignoring this cartridge's HeatingAccelInCarousel heat loss curve @13:49:21.00 -> Cmd.startFiltering @13:49:21.44 Priming sample loop w/100ml, bypass w/10ml @13:49:21.46 SamplePump.setPosition! 0ml @13:49:21.50 Bypass.open @13:49:22.99 Intake.open @13:49:23.26 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:49:25.82 Vacuum tare is 51% of full scale @13:49:25.95 Pressure.in :default @13:49:26.00 Pressure.hold @13:49:26.19 SamplePump.in :prime @13:49:26.25 SamplePump.seek 10ml,59 seconds @13:49:35.51 Pressure.stop @13:49:35.56 SamplePump.setPosition! 0ml @13:49:35.61 Exhaust.open @13:49:37.04 Bypass.close @13:49:38.56 Pressure.in :default @13:49:38.62 Pressure.hold @13:49:38.83 SamplePump.in :prime @13:49:38.91 SamplePump.seek 100ml,5:16 @13:51:42.79 Pressure.stop @13:51:42.85 Bypass.open @13:51:43.56 Intake.close @13:51:44.39 Toroid.in :hold @13:51:44.45 Toroid.seek :crack @13:51:45.96 Toroid.seek :cartridge @13:51:47.04 Toroid.in :default @13:51:47.10 Toroid.seek :clear @13:51:49.05 Pressurizing cartridge to 14psi above 14.1psia @13:51:49.07 SamplePump.setPosition! 0ml @13:51:49.11 Intake.open @13:51:49.25 Bypass.close @13:51:50.00 Exhaust.close @13:51:50.83 Pressure.in :default @13:51:50.89 Pressure.hold @13:51:51.06 SamplePump.in :charge @13:51:51.13 SamplePump.seek 2.3ml,41 seconds @13:52:00.74 Pressure.stop @13:52:00.79 Intake.close @13:52:01.62 Pressurized cartridge with 0.412ml to 14.1psi @13:52:01.63 Isolated @24.9psia (10.8psi) @13:52:01.65 Verifying cartridge holds at least 0.75..0.85 of 24.9psia @13:52:01.68 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:52:06.30 Sampling 75.0ml @13:52:06.32 SamplePump.setPosition! 0ml @13:52:06.37 Exhaust.open @13:52:07.09 Intake.open @13:52:07.94 Pressure.in :default @13:52:07.99 Pressure.hold @13:52:08.22 SamplePump.in :sample @13:52:08.28 SamplePump.seek 75ml,5:31 @13:52:10.56 Cartridge 43 filtering 1/75ml at 0.35ml/s with 12.4psi vacuum @13:53:10.56 Cartridge 43 filtering 21/75ml at 0.32ml/s with 14.8psi vacuum @13:54:10.56 Cartridge 43 filtering 40/75ml at 0.31ml/s with 13.2psi vacuum @13:55:10.56 Cartridge 43 filtering 60/75ml at 0.31ml/s with 12.0psi vacuum @13:55:59.79 Pressure.stop @13:55:59.91 Intake.close @13:55:59.97 Exhaust.close @13:56:00.12 Toroid.in :hold @13:56:00.19 Toroid.seek :bypass @13:56:01.80 Toroid.in :default @13:56:01.86 Toroid.seek :clear @13:56:03.48 Sampled 75.0ml @13:56:05.02 -> Cmd.startProcessing @13:56:06.55 SPR.command \"read_sequences\" @13:56:06.60 SPR.command \"stats_on\" @13:56:06.64 SPR.command \"start 0 1\" @13:56:07.45 SPR.start /var/log/esp/spr21aug0713h.spr.bz2 @13:56:09.45 Handoff.select :waste @13:56:16.13 Syringe tare is 19% of full scale @13:56:16.15 PinPort.in :locking @13:56:16.22 PinPort.seek :locked @13:56:16.71 Archive.in :fast @13:56:16.75 Lysis2.in :fast @13:56:16.81 Lysis1.seek 1350uL @13:56:16.89 Archive.seek :full @13:56:16.96 Lysis2.seek :full @13:56:19.06 Plunge.seek :reagent @13:56:19.96 Evacuating puck with filter bubble point of 24psi @13:56:19.98 PinPort.seek :locked @13:56:20.06 Handoff.select :waste @13:56:26.65 Air syringe pumping up to 1 stroke to reach 8psi @13:56:26.66 Air stroke #1 of 1 @13:56:26.75 Air.in :pressurize @13:56:26.81 Air.seek :pressurized @13:56:38.06 1.3psi after pressurize @13:56:38.07 Air.in :compress @13:56:38.15 Air.seek :evac @13:56:42.23 1.2psi after compress @13:56:54.80 0.4psi after compress (stable) @13:56:54.82 Air.in :default @13:56:54.89 Air.hold @13:56:55.05 at 0.4psi Opened to waste @13:56:55.07 Handoff.select :blocked @13:56:55.20 Archive syringe pumping up to 8 strokes to reach 24psi @13:56:55.21 Archive stroke #1 of 8 @13:56:55.29 Archive.in :pressurize @13:56:55.36 Archive.seek :pressurized @13:57:08.10 2.9psi after pressurize @13:57:08.12 Archive.in :compress @13:57:08.19 Archive.seek :empty @13:57:24.06 15.3psi after compress @13:57:36.64 15.9psi after compress (stable) @13:57:36.65 Archive.in :gulp @13:57:36.73 Archive.seek :topVent @13:57:43.93 16.0psi after gulp @13:57:56.51 15.9psi after gulp (stable) @13:57:56.52 Archive stroke #2 of 8 @13:57:56.60 Archive.in :pressurize @13:57:56.67 Archive.seek :pressurized @13:58:09.40 15.9psi after pressurize @13:58:09.42 Archive.in :compress @13:58:09.49 Archive.seek :empty @13:58:21.99 Reached 24.1 of 24.0psi after pumping 3388uL (1.96 strokes) @13:58:22.00 Archive.in :default @13:58:22.07 Archive.hold @13:58:22.16 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position @13:58:22.18 Handoff.select :waste @13:58:35.87 delay 1:00 @13:59:35.90 Handoff.select :blocked @13:59:58.58 Handoff.select :waste @13:59:58.70 0.3psi at evacEnd @13:59:58.71 Handoff.select :blocked @13:59:58.84 Archive.in :fast @13:59:58.92 Archive.seek :topVent @14:00:12.37 Stable @0.3psi after Handoff blocked @14:00:12.39 Archive syringe pumping up to 8 strokes to reach 15psi @14:00:12.41 Archive stroke #1 of 8 @14:00:12.50 Archive.in :pressurize @14:00:12.56 Archive.seek :pressurized @14:00:25.30 0.5psi after pressurize @14:00:25.34 Archive.in :compress @14:00:25.41 Archive.seek :empty @14:00:41.30 13.8psi after compress @14:00:53.87 14.4psi after compress (stable) @14:00:53.89 Archive.in :gulp @14:00:53.96 Archive.seek :topVent @14:01:01.16 14.5psi after gulp @14:01:14.17 14.5psi after gulp (stable) @14:01:14.18 Archive stroke #2 of 8 @14:01:14.27 Archive.in :pressurize @14:01:14.33 Archive.seek :pressurized @14:01:27.06 14.4psi after pressurize @14:01:27.08 Archive.in :compress @14:01:27.15 Archive.seek :empty @14:01:32.03 Reached 15.4 of 15.3psi after pumping 3225uL (1.86 strokes) @14:01:32.04 Archive.in :default @14:01:32.11 Archive.hold @14:01:32.18 Handoff.select :waste @14:01:39.37 delay 1:00 @14:02:39.39 Handoff.select :blocked @14:03:02.06 Handoff.select :waste @14:03:02.18 0.2psi at evacEnd @14:03:02.22 Air.seek :fullVent @14:03:03.71 Archive.in :fast @14:03:03.77 Archive.seek :topVent @14:03:10.08 Seawater evacuated, Presure above/below filter released. Lysis begins @14:03:10.17 Lysis1.in :slow @14:03:10.23 Lysis1.seek 1050uL @14:03:40.23 delay 5 seconds @14:03:45.25 Handoff.select :blocked @14:03:45.42 Lysis1.seek 450uL @14:04:45.36 delay 3 seconds @14:04:48.43 Air.in to mix into 1.37806 parts Lysis1 @14:04:49.00 Air.seek :evac @14:04:49.04 Lysis1.seek 300uL @14:05:04.14 Lysis1.seek :empty @14:05:34.22 Lysis buffer delivered @22.5psi @14:05:34.23 Polling Can every 2:00 @14:05:34.78 PuckHeater.configure :default #ambient 24.5C @14:05:34.83 PuckHeater.seek 79.6C @14:06:21.23 PuckHeater.hold 2:00,79.6C @14:08:21.07 Polling Can every 2:00 @14:08:21.63 PuckHeater.configure :default #ambient 24.6C @14:08:21.68 PuckHeater.hold 30 seconds,78.6C @14:08:51.80 Polling Can every 2:00 @14:08:52.35 PuckHeater.configure :default #ambient 24.6C @14:08:52.40 PuckHeater.hold 30 seconds,78.1C @14:09:22.52 Polling Can every 2:00 @14:09:23.08 PuckHeater.configure :default #ambient 24.6C @14:09:23.13 PuckHeater.hold 10:00,77.6C @14:19:21.63 Lysis complete. Syringe pressure @24.8psi @14:19:21.64 Handoff.select :blocked @14:19:21.68 Archive syringe pumping up to 5 strokes to reach 40psi @14:19:21.69 Archive stroke #1 of 5 @14:19:21.78 Archive.in :pressurize @14:19:21.85 Archive.seek :pressurized @14:19:34.60 24.5psi after pressurize @14:19:34.62 Archive.in :compress @14:19:34.69 Archive.seek :empty @14:19:40.03 Reached 40.5 of 40.0psi after pumping 1504uL (0.87 strokes) @14:19:40.04 Archive.in :default @14:19:40.11 Archive.hold @14:19:40.19 delay 2 seconds @14:19:42.21 Archive.in :fast @14:19:42.28 Archive.seek :full @14:19:48.70 Lysis1.seek 138uL @14:20:02.59 delay 1 second @14:20:03.62 Archive.seek :full @14:20:03.64 Lysis1.seek :empty @14:20:17.62 Initial air pressure @45.9psi @14:20:17.69 Lysis1.in :extract @14:20:17.76 Lysis1.seek 600uL,nil @14:20:21.67 Archive syringe pumping up to 6 strokes to reach 25psi @14:20:21.68 Archive stroke #1 of 6 @14:20:21.78 Archive.in :pressurize @14:20:21.86 Archive.seek :pressurized @14:20:34.62 14.4psi after pressurize @14:20:34.64 Archive.in :compress @14:20:34.71 Archive.seek :empty @14:20:41.82 Syringe::LysatePlungerStuck in PROCESSING -- at < 499uL during Lysis Syringe #1 (LyS1) move @14:20:41.85 Lysis1.in :default @14:20:41.92 Lysis1.seek 464uL @14:20:43.63 Reached 25.2 of 25.0psi after pumping 1580uL (0.91 strokes) @14:20:43.65 Archive.in :default @14:20:43.73 Archive.hold @14:20:43.92 Lysis1.in :extract @14:20:43.99 Lysis1.seek 600uL,nil @14:20:46.62 Initial 600uL lysate @21.9psi @14:20:46.63 Handoff.select :blocked @14:20:46.72 Initial air pressure @22.2psi @14:20:46.79 Lysis1.seek 825uL,nil @14:20:51.04 Recovered 825uL lysate @16.2psi @14:20:51.05 Air.in :halfSpeed @14:20:51.13 Air.seek :fullVent @14:20:53.39 Puck Top Vented, bottom @9.7psi @14:20:53.40 Handoff.select :waste @14:20:53.52 delay 5 seconds @14:20:58.60 Handoff open to waste, bottom @0.1psi @14:20:58.61 Air.in :default @14:20:58.68 Air.seek :evac @14:21:00.15 Lysis2.in :DAmix @14:21:00.25 Lysis1.in :DAmix @14:21:00.80 Lysis2.seek 1480uL @14:21:00.84 Lysis1.seek 575uL @14:21:35.91 delay 5 seconds @14:21:40.93 Handoff.select SPR @14:21:41.06 SPR.command \"start 2 14\" @14:21:41.74 SPR stabilized @14:21:41.87 delay 10 seconds @14:21:51.89 SPR.command \"start 2 0\" @14:21:55.10 Waiting up to 11:00 for SPR to enter state 1 @14:23:52.77 SPR.command \"hold_on\" @14:23:54.16 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @14:23:54.69 Lysis2.seek 1180uL @14:23:54.73 Lysis1.seek 275uL @14:24:36.74 delay 15 seconds @14:24:51.76 SPR.command \"hold_off\" @14:24:55.32 Waiting up to 38:00 for SPR to enter state 7 @14:48:52.90 SPR analysis completed @14:48:57.12 Ended log spr21aug0713h.spr.bz2 @14:48:57.13 SPR.command \"stats_off\" @14:48:58.58 -> Cmd.stop @14:48:58.67 Handoff.select :close @14:48:58.79 Gate.power :heat,:OFF @14:48:58.81 Gate.power :cartridge,:OFF @14:48:58.89 Carousel.seek 43 @14:48:59.06 Plunge.seek :locked @14:48:59.94 PinPort.in :unlocking @14:49:00.01 PinPort.seek :clear @14:49:00.55 Archive.in :unlocking @14:49:00.67 Archive.seek :clear @14:49:00.75 Air.in :unlocking @14:49:00.86 Lysis1.in :unlocking @14:49:00.90 Air.seek :clear @14:49:01.01 Lysis1.seek :clear @14:49:01.14 Lysis2.in :unlocking @14:49:01.21 Lysis2.seek :clear @14:49:04.82 Gate.power :motor,:OFF @14:49:04.84 Gate.power :core,:OFF @14:49:04.86 Gate.power :analytic1,:OFF @14:49:04.88 Gate.power :analytic2,:OFF @14:49:04.90 Gate.power :analytic3,:OFF @14:49:04.92 Gate.power :main,:OFF @14:49:04.95 Safely stopped and ready to power off ##-- file closed 2021-08-07T23:51:08.988Z ##-- file opened 2021-08-07T23:51:08.990Z @19:51:09.63 -> showStatus @19:51:11.65 -> Cmd.loadCartridge -4 @19:51:11.70 Gate.power :main,:ON,limit to 5000mA @19:51:11.72 Gate.power :analytic1,:ON,limit to 5000mA @19:51:11.74 Gate.power :analytic2,:ON,limit to 5000mA @19:51:11.77 Gate.power :analytic3,:ON,limit to 5000mA @19:51:11.90 Powering core bus @19:51:14.19 SPR Module 1, FW Rev. B, FPGA Rev. C @19:51:14.64 Archive.configure :default @19:51:14.76 Air.configure :default @19:51:14.87 Lysis1.configure :default @19:51:14.97 Lysis2.configure :default @19:51:15.09 Plunge.configure :default @19:51:15.21 SamplePump.configure :default @19:51:15.36 ExtraPump.configure :default @19:51:15.47 Toroid.configure :default @19:51:15.60 Carousel.configure :default @19:51:15.73 Pressure.configure :default @19:51:15.83 PinPort.configure :default @19:51:15.90 All elf controllers running firmware version 4.741 @19:51:15.93 Gate.power :motor,:ON,limit to 5000mA @19:51:16.56 Archive.in :default @19:51:16.74 Archive.home.jog 0 @19:51:16.91 Toroid.in :default @19:51:16.99 Plunge.in :default @19:51:17.02 Lysis1.in :default @19:51:17.07 PinPort.in :default @19:51:17.13 Lysis2.in :default @19:51:17.17 Air.in :default @19:51:17.33 Toroid.home.jog 0 @19:51:17.37 PinPort.home.jog 0 @19:51:17.39 Lysis1.home.jog 0 @19:51:17.42 Plunge.home.jog 0 @19:51:17.50 Lysis2.home.jog 0 @19:51:17.59 Air.home.jog 0 @19:51:17.92 Archive.in :homing @19:51:18.08 Toroid.in :homing @19:51:18.11 Plunge.in :homing @19:51:18.15 Archive.jog 1397 @19:51:18.21 Lysis1.in :homing @19:51:18.24 PinPort.in :homing @19:51:18.43 Toroid.jog 627 @19:51:18.47 Plunge.jog 1564 @19:51:18.50 PinPort.jog -108 @19:51:18.53 Lysis1.jog 1411 @19:51:18.73 Air.in :homing @19:51:18.75 Lysis2.in :homing @19:51:18.84 Air.jog 1414 @19:51:18.87 Lysis2.jog 1407 @19:51:19.00 PinPort.setRawPosition! 0 @19:51:19.05 PinPort.seek :home @19:51:19.13 PinPort.in :default @19:51:19.50 Archive.setRawPosition! 50 @19:51:19.54 Archive.seek :home @19:51:19.79 Lysis1.setRawPosition! 50 @19:51:19.85 Lysis1.seek :home @19:51:19.94 Archive.in :default @19:51:19.96 Plunge.setRawPosition! 50 @19:51:20.04 Plunge.seek :home @19:51:20.18 Lysis2.setRawPosition! 50 @19:51:20.20 Lysis1.in :default @19:51:20.23 Air.setRawPosition! 50 @19:51:20.33 Lysis2.seek :home @19:51:20.40 Air.seek :home @19:51:20.52 Plunge.in :default @19:51:20.69 Toroid.setRawPosition! 0 @19:51:20.75 Toroid.seek :home @19:51:20.85 Lysis2.in :default @19:51:20.88 Air.in :default @19:51:20.91 Toroid.in :default @19:51:21.37 Toroid.seek :clear @19:51:22.99 Carousel.in :default @19:51:23.05 Carousel.home.jog 0 @19:51:23.54 Carousel.in :default @19:51:23.60 Carousel.seek 43 @19:51:23.77 Selecting Cartridge 42 @19:51:24.01 Carousel.seek 42 @19:51:24.51 Lysis1.in :fast @19:51:24.57 Lysis1.seek :locked @19:51:27.16 Plunge.seek :locked @19:51:27.28 Air.in :fast @19:51:27.35 Air.seek :locked @19:51:29.94 Powering cartridge bus @19:51:32.56 Gate.setIR 1750,5000,0,6 @19:51:32.69 Gate.setIR 0,5000,0,6 @19:51:32.74 Gate.power :cartridge,:OFF @19:51:33.28 Mounted archive Cartridge @19:51:51.24 -> Cmd.startFiltering @19:51:51.69 Priming sample loop w/100ml, bypass w/10ml @19:51:51.71 SamplePump.setPosition! 0ml @19:51:51.75 Bypass.open @19:51:53.26 Intake.open @19:51:53.49 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @19:51:56.05 Vacuum tare is 50% of full scale @19:51:56.18 Pressure.in :default @19:51:56.24 Pressure.hold @19:51:56.42 SamplePump.in :prime @19:51:56.49 SamplePump.seek 10ml,59 seconds @19:52:05.74 Pressure.stop @19:52:05.79 SamplePump.setPosition! 0ml @19:52:05.84 Exhaust.open @19:52:07.26 Bypass.close @19:52:08.77 Pressure.in :default @19:52:08.83 Pressure.hold @19:52:09.04 SamplePump.in :prime @19:52:09.12 SamplePump.seek 100ml,5:16 @19:54:12.68 Pressure.stop @19:54:12.74 Bypass.open @19:54:13.47 Intake.close @19:54:14.30 Toroid.in :hold @19:54:14.36 Toroid.seek :crack @19:54:15.88 Toroid.seek :cartridge @19:54:16.97 Toroid.in :default @19:54:17.03 Toroid.seek :clear @19:54:18.99 Pressurizing cartridge to 26psi above 14.8psia @19:54:19.00 SamplePump.setPosition! 0ml @19:54:19.04 Intake.open @19:54:19.22 Bypass.close @19:54:19.97 Exhaust.close @19:54:20.75 Pressure.in :default @19:54:20.81 Pressure.hold @19:54:20.99 SamplePump.in :charge @19:54:21.05 SamplePump.seek 2.3ml,42 seconds @19:54:27.53 Pressure.stop @19:54:27.58 Intake.close @19:54:28.45 Pressurized cartridge with 0.185ml to 28.3psi @19:54:28.47 Isolated @37.2psia (22.3psi) @19:54:28.48 Verifying cartridge holds at least 0.75..0.85 of 37.2psia @19:54:28.52 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @19:54:33.09 Cartridge pressure fell 1% to 36.8psia @19:54:33.17 Sampling 1000.0ml @19:54:33.18 SamplePump.setPosition! 0ml @19:54:33.24 Exhaust.open @19:54:33.93 Intake.open @19:54:34.28 Pressure.in :default @19:54:34.63 Pressure.hold @19:54:34.97 SamplePump.in :sample @19:54:35.04 SamplePump.seek 1000ml,1:23:53 @19:54:37.31 Cartridge 42 filtering 1/1000ml at 0.35ml/s with 26.9psi vacuum @19:55:37.31 Cartridge 42 filtering 23/1000ml at 0.34ml/s with 21.8psi vacuum @19:56:37.32 Cartridge 42 filtering 44/1000ml at 0.31ml/s with 25.0psi vacuum @19:57:37.31 Cartridge 42 filtering 65/1000ml at 0.35ml/s with 29.4psi vacuum @19:58:37.32 Cartridge 42 filtering 85/1000ml at 0.28ml/s with 18.8psi vacuum @19:59:37.31 Cartridge 42 filtering 103/1000ml at 0.26ml/s with 26.9psi vacuum @20:00:37.32 Cartridge 42 filtering 121/1000ml at 0.28ml/s with 21.0psi vacuum @20:01:37.31 Cartridge 42 filtering 137/1000ml at 0.28ml/s with 24.3psi vacuum @20:02:37.32 Cartridge 42 filtering 152/1000ml at 0.25ml/s with 21.7psi vacuum @20:03:37.31 Cartridge 42 filtering 167/1000ml at 0.20ml/s with 24.5psi vacuum @20:04:37.32 Cartridge 42 filtering 181/1000ml at 0.23ml/s with 23.4psi vacuum @20:05:37.31 Cartridge 42 filtering 194/1000ml at 0.24ml/s with 24.3psi vacuum @20:06:14.22 Flow averaged 0.29ml/s over 11:40 @20:06:14.25 Waiting up to 10 seconds for vacuum to reduce from 27.0 to 18.0psi @20:06:14.82 Relieved excess vacuum in .6 seconds @20:06:14.85 SamplePump.seek 1000ml,1:07:04 @20:06:17.84 Flow estimate reduced to 0.23ml/s @20:06:17.88 Waiting up to 16 seconds for vacuum to reduce from 29.3 to 18.0psi @20:06:18.44 Relieved excess vacuum in .6 seconds @20:06:18.47 SamplePump.seek 1000ml,1:07:01 @20:06:32.07 Flow averaged 0.20ml/s over 14 seconds @20:06:32.10 Waiting up to 17 seconds for vacuum to reduce from 29.7 to 18.0psi @20:06:32.68 Relieved excess vacuum in .6 seconds @20:06:32.70 SamplePump.seek 1000ml,1:06:48 @20:06:34.67 Flow estimate reduced to 0.16ml/s @20:06:34.69 Intake.close @20:06:34.73 Exhaust.close @20:06:34.92 Toroid.in :hold @20:06:34.99 Toroid.seek :bypass @20:06:36.62 Toroid.in :default @20:06:36.68 Toroid.seek :clear @20:06:37.32 Cartridge 42 filtering 205/1000ml at 0.00ml/s with 1.0psi vacuum @20:06:38.30 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @20:06:38.31 Pressure.stop @20:06:38.50 Sampled 205.3ml @20:06:40.48 -> Cmd.startProcessing @20:06:40.86 Plunge.seek :reagent @20:06:41.69 Sample loop closed. Ready to Deliver Reagents @20:06:41.70 Archive.seek :sealed @20:06:45.97 Archive.seek :evac @20:06:59.90 delay 1:00 @20:07:59.92 Archive.seek :clear @20:08:17.64 RNAlater soak for 10:00 @20:18:17.66 Archive.seek :tVent @20:18:20.24 delay 1 second @20:18:21.26 Archive.seek :evac @20:18:36.88 delay 10 seconds @20:18:46.91 Archive.seek :tVent @20:19:02.53 delay 1 second @20:19:03.55 Archive.seek :evac @20:19:19.18 delay 10 seconds @20:19:29.21 Sample preserved and evacuated @20:19:30.15 -> Cmd.stop @20:19:30.29 Carousel.seek 42 @20:19:30.46 Plunge.seek :locked @20:19:31.41 Archive.in :unlocking @20:19:31.54 Archive.seek :clear @20:19:31.58 Air.in :unlocking @20:19:31.71 Air.seek :clear @20:19:31.80 Lysis1.in :unlocking @20:19:31.92 Lysis1.seek :clear @20:19:35.79 Gate.power :motor,:OFF @20:19:35.81 Gate.power :core,:OFF @20:19:35.83 Gate.power :analytic1,:OFF @20:19:35.85 Gate.power :analytic2,:OFF @20:19:35.88 Gate.power :analytic3,:OFF @20:19:35.90 Gate.power :main,:OFF @20:19:35.92 Safely stopped and ready to power off ##-- file closed 2021-08-08T00:28:32.870Z ##-- file opened 2021-08-08T00:28:32.872Z @20:28:32.92 -> showStatus @20:28:34.94 -> Cmd.loadCartridge -18 @20:28:35.00 Gate.power :main,:ON,limit to 5000mA @20:28:35.02 Gate.power :analytic1,:ON,limit to 5000mA @20:28:35.04 Gate.power :analytic2,:ON,limit to 5000mA @20:28:35.08 Gate.power :analytic3,:ON,limit to 5000mA @20:28:35.23 Powering core bus @20:28:37.51 SPR Module 1, FW Rev. B, FPGA Rev. C @20:28:37.98 Archive.configure :default @20:28:38.10 Air.configure :default @20:28:38.20 Lysis1.configure :default @20:28:38.31 Lysis2.configure :default @20:28:38.43 Plunge.configure :default @20:28:38.55 SamplePump.configure :default @20:28:38.70 ExtraPump.configure :default @20:28:38.81 Toroid.configure :default @20:28:38.93 Carousel.configure :default @20:28:39.05 Pressure.configure :default @20:28:39.16 PinPort.configure :default @20:28:39.58 All elf controllers running firmware version 4.741 @20:28:39.61 Gate.power :motor,:ON,limit to 5000mA @20:28:39.89 Archive.in :default @20:28:40.10 Archive.home.jog 0 @20:28:40.27 PinPort.in :default @20:28:40.31 Toroid.in :default @20:28:40.34 Plunge.in :default @20:28:40.37 Lysis1.in :default @20:28:40.41 Air.in :default @20:28:40.45 Lysis2.in :default @20:28:40.61 PinPort.home.jog 0 @20:28:40.66 Toroid.home.jog 0 @20:28:40.69 Lysis1.home.jog 0 @20:28:40.72 Plunge.home.jog 0 @20:28:40.76 Air.home.jog 0 @20:28:40.79 Lysis2.home.jog 0 @20:28:41.34 PinPort.in :homing @20:28:41.38 Toroid.in :homing @20:28:41.47 Lysis1.in :homing @20:28:41.51 Archive.in :homing @20:28:41.54 Plunge.in :homing @20:28:41.71 Toroid.jog 626 @20:28:41.76 PinPort.jog -109 @20:28:41.79 Plunge.jog 1558 @20:28:41.81 Lysis1.jog 1411 @20:28:41.84 Archive.jog 1399 @20:28:42.10 Air.in :homing @20:28:42.13 Lysis2.in :homing @20:28:42.22 Lysis2.jog 1409 @20:28:42.24 Air.jog 1410 @20:28:42.35 PinPort.setRawPosition! 0 @20:28:42.39 PinPort.seek :home @20:28:42.48 PinPort.in :default @20:28:43.09 Lysis1.setRawPosition! 50 @20:28:43.15 Lysis1.seek :home @20:28:43.24 Plunge.setRawPosition! 50 @20:28:43.25 Archive.setRawPosition! 50 @20:28:43.32 Plunge.seek :home @20:28:43.36 Archive.seek :home @20:28:43.51 Lysis1.in :default @20:28:43.54 Lysis2.setRawPosition! 50 @20:28:43.57 Air.setRawPosition! 50 @20:28:43.65 Lysis2.seek :home @20:28:43.74 Air.seek :home @20:28:43.86 Plunge.in :default @20:28:43.89 Archive.in :default @20:28:44.01 Toroid.setRawPosition! 0 @20:28:44.08 Toroid.seek :home @20:28:44.19 Lysis2.in :default @20:28:44.22 Air.in :default @20:28:44.25 Toroid.in :default @20:28:44.68 Toroid.seek :clear @20:28:46.30 Carousel.in :default @20:28:46.37 Carousel.home.jog 0 @20:28:46.85 Carousel.in :default @20:28:46.91 Carousel.seek 42 @20:28:47.06 Selecting Cartridge 41 @20:28:47.31 Carousel.seek 41 @20:28:47.80 Lysis1.in :fast @20:28:47.86 Lysis1.seek :locked @20:28:50.45 Plunge.seek :locked @20:28:50.57 Air.in :fast @20:28:50.63 Air.seek :locked @20:28:53.24 Powering cartridge bus @20:28:55.86 Gate.setIR 1750,5000,0,6 @20:28:55.99 Gate.setIR 0,5000,0,6 @20:28:56.05 PuckHeater.configure :default @20:28:56.11 Gate.power :heat,:ON,limit to 5000mA @20:28:56.85 Mounted lysis Cartridge board#Q05044 @20:28:56.89 Ignoring this cartridge's HeatingAccelInCarousel heat loss curve @20:29:15.37 -> Cmd.startFiltering @20:29:15.82 Priming sample loop w/100ml, bypass w/10ml @20:29:15.84 SamplePump.setPosition! 0ml @20:29:15.88 Bypass.open @20:29:17.37 Intake.open @20:29:17.62 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @20:29:20.18 Vacuum tare is 51% of full scale @20:29:20.30 Pressure.in :default @20:29:20.36 Pressure.hold @20:29:20.55 SamplePump.in :prime @20:29:20.61 SamplePump.seek 10ml,59 seconds @20:29:29.87 Pressure.stop @20:29:29.92 SamplePump.setPosition! 0ml @20:29:29.97 Exhaust.open @20:29:31.39 Bypass.close @20:29:32.93 Pressure.in :default @20:29:32.99 Pressure.hold @20:29:33.20 SamplePump.in :prime @20:29:33.27 SamplePump.seek 100ml,5:16 @20:31:37.10 Pressure.stop @20:31:37.16 Bypass.open @20:31:37.87 Intake.close @20:31:38.73 Toroid.in :hold @20:31:38.79 Toroid.seek :crack @20:31:40.30 Toroid.seek :cartridge @20:31:41.38 Toroid.in :default @20:31:41.44 Toroid.seek :clear @20:31:43.39 Pressurizing cartridge to 14psi above 10.4psia @20:31:43.40 SamplePump.setPosition! 0ml @20:31:43.44 Intake.open @20:31:44.16 Bypass.close @20:31:44.86 Exhaust.close @20:31:45.63 Pressure.in :default @20:31:45.69 Pressure.hold @20:31:45.86 SamplePump.in :charge @20:31:45.92 SamplePump.seek 2.3ml,41 seconds @20:31:57.56 Pressure.stop @20:31:57.61 Intake.close @20:31:58.45 Pressurized cartridge with 0.606ml to 14.1psi @20:31:58.46 Isolated @20.2psia (9.8psi) @20:31:58.47 Verifying cartridge holds at least 0.75..0.85 of 20.2psia @20:31:58.51 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @20:32:03.08 Cartridge pressure fell 4% to 19.5psia @20:32:03.16 Sampling 50.0ml @20:32:03.17 SamplePump.setPosition! 0ml @20:32:03.22 Exhaust.open @20:32:04.30 Intake.open @20:32:04.67 Pressure.in :default @20:32:04.73 Pressure.hold @20:32:04.95 SamplePump.in :sample @20:32:05.02 SamplePump.seek 50ml,3:51 @20:32:07.70 Cartridge 41 filtering 1/50ml at 0.34ml/s with 14.7psi vacuum @20:33:07.70 Cartridge 41 filtering 21/50ml at 0.35ml/s with 11.2psi vacuum @20:34:07.70 Cartridge 41 filtering 41/50ml at 0.32ml/s with 12.6psi vacuum @20:34:35.01 Pressure.stop @20:34:35.14 Intake.close @20:34:35.19 Exhaust.close @20:34:35.34 Toroid.in :hold @20:34:35.40 Toroid.seek :bypass @20:34:37.02 Toroid.in :default @20:34:37.08 Toroid.seek :clear @20:34:38.70 Sampled 50.0ml @20:34:40.28 -> Cmd.startProcessing @20:34:41.79 SPR.command \"read_sequences\" @20:34:42.45 SPR.command \"stats_on\" @20:34:42.49 SPR.command \"start 0 1\" @20:34:43.30 SPR.start /var/log/esp/spr21aug0720h.spr.bz2 @20:34:45.30 Handoff.select :waste @20:34:51.99 Syringe tare is 19% of full scale @20:34:52.02 PinPort.in :locking @20:34:52.09 PinPort.seek :locked @20:34:52.57 Archive.in :fast @20:34:52.63 Lysis2.in :fast @20:34:52.67 Lysis1.seek 1350uL @20:34:52.80 Archive.seek :full @20:34:52.84 Lysis2.seek :full @20:34:54.91 Plunge.seek :reagent @20:34:55.81 Evacuating puck with filter bubble point of 24psi @20:34:55.83 PinPort.seek :locked @20:34:55.92 Handoff.select :waste @20:35:03.00 Air syringe pumping up to 1 stroke to reach 8psi @20:35:03.01 Air stroke #1 of 1 @20:35:03.10 Air.in :pressurize @20:35:03.16 Air.seek :pressurized @20:35:14.42 1.3psi after pressurize @20:35:14.43 Air.in :compress @20:35:14.51 Air.seek :evac @20:35:18.59 1.4psi after compress @20:35:31.16 0.4psi after compress (stable) @20:35:31.19 Air.in :default @20:35:31.27 Air.hold @20:35:31.44 at 0.4psi Opened to waste @20:35:31.45 Handoff.select :blocked @20:35:31.58 Archive syringe pumping up to 8 strokes to reach 24psi @20:35:31.59 Archive stroke #1 of 8 @20:35:31.67 Archive.in :pressurize @20:35:31.73 Archive.seek :pressurized @20:35:44.48 3.4psi after pressurize @20:35:44.51 Archive.in :compress @20:35:44.58 Archive.seek :empty @20:36:00.47 14.8psi after compress @20:36:13.05 15.6psi after compress (stable) @20:36:13.07 Archive.in :gulp @20:36:13.14 Archive.seek :topVent @20:36:20.34 15.6psi after gulp @20:36:32.92 15.6psi after gulp (stable) @20:36:32.93 Archive stroke #2 of 8 @20:36:33.02 Archive.in :pressurize @20:36:33.09 Archive.seek :pressurized @20:36:45.82 15.5psi after pressurize @20:36:45.83 Archive.in :compress @20:36:45.93 Archive.seek :empty @20:36:57.44 Reached 24.1 of 24.0psi after pumping 3367uL (1.95 strokes) @20:36:57.45 Archive.in :default @20:36:57.52 Archive.hold @20:36:57.58 Handoff.select :waste @20:37:06.28 delay 1:00 @20:38:06.30 Handoff.select :blocked @20:38:29.00 Handoff.select :waste @20:38:29.13 0.2psi at evacEnd @20:38:29.14 Handoff.select :blocked @20:38:29.27 Archive.in :fast @20:38:29.34 Archive.seek :topVent @20:38:42.73 Stable @0.2psi after Handoff blocked @20:38:42.75 Archive syringe pumping up to 8 strokes to reach 15psi @20:38:42.76 Archive stroke #1 of 8 @20:38:42.85 Archive.in :pressurize @20:38:42.92 Archive.seek :pressurized @20:38:55.65 0.6psi after pressurize @20:38:55.67 Archive.in :compress @20:38:55.74 Archive.seek :empty @20:39:11.64 13.2psi after compress @20:39:24.21 13.7psi after compress (stable) @20:39:24.23 Archive.in :gulp @20:39:24.30 Archive.seek :topVent @20:39:31.52 13.7psi after gulp @20:39:44.09 13.6psi after gulp (stable) @20:39:44.10 Archive stroke #2 of 8 @20:39:44.19 Archive.in :pressurize @20:39:44.25 Archive.seek :pressurized @20:39:57.01 13.6psi after pressurize @20:39:57.02 Archive.in :compress @20:39:57.09 Archive.seek :empty @20:40:00.98 Reached 15.3 of 15.2psi after pumping 3204uL (1.85 strokes) @20:40:00.99 Archive.in :default @20:40:01.06 Archive.hold @20:40:01.12 Handoff.select :waste @20:40:10.81 delay 1:00 @20:41:10.83 Handoff.select :blocked @20:41:33.51 Handoff.select :waste @20:41:33.63 0.2psi at evacEnd @20:41:33.65 Air.seek :fullVent @20:41:35.09 Archive.in :fast @20:41:35.15 Archive.seek :topVent @20:41:41.40 Seawater evacuated, Presure above/below filter released. Lysis begins @20:41:41.49 Lysis1.in :slow @20:41:41.55 Lysis1.seek 1050uL @20:42:11.57 delay 5 seconds @20:42:16.59 Handoff.select :blocked @20:42:16.77 Lysis1.seek 450uL @20:43:16.69 delay 3 seconds @20:43:19.76 Air.in to mix into 1.37806 parts Lysis1 @20:43:20.33 Air.seek :evac @20:43:20.38 Lysis1.seek 300uL @20:43:35.49 Lysis1.seek :empty @20:44:05.59 Lysis buffer delivered @17.8psi @20:44:05.60 Polling Can every 2:00 @20:44:06.15 PuckHeater.configure :default #ambient 25.1C @20:44:06.19 PuckHeater.seek 79.6C @20:44:50.50 PuckHeater.hold 2:00,79.6C @20:46:50.37 Polling Can every 2:00 @20:46:50.91 PuckHeater.configure :default #ambient 25.2C @20:46:50.95 PuckHeater.hold 30 seconds,78.6C @20:47:21.10 Polling Can every 2:00 @20:47:21.64 PuckHeater.hold 30 seconds,78.1C @20:47:51.81 Polling Can every 2:00 @20:47:52.36 PuckHeater.configure :default #ambient 25.2C @20:47:52.41 PuckHeater.hold 10:00,77.5C @20:57:51.71 Lysis complete. Syringe pressure @19.2psi @20:57:51.72 Handoff.select :blocked @20:57:51.76 Archive syringe pumping up to 5 strokes to reach 40psi @20:57:51.77 Archive stroke #1 of 5 @20:57:51.85 Archive.in :pressurize @20:57:51.92 Archive.seek :pressurized @20:58:04.65 19.2psi after pressurize @20:58:04.66 Archive.in :compress @20:58:04.77 Archive.seek :empty @20:58:11.64 Reached 40.3 of 40.0psi after pumping 1538uL (0.89 strokes) @20:58:11.65 Archive.in :default @20:58:11.72 Archive.hold @20:58:11.79 delay 2 seconds @20:58:13.81 Archive.in :fast @20:58:13.87 Archive.seek :full @20:58:20.39 Lysis1.seek 138uL @20:58:34.26 delay 1 second @20:58:35.29 Archive.seek :full @20:58:35.31 Lysis1.seek :empty @20:58:49.28 Initial air pressure @45.7psi @20:58:49.35 Lysis1.in :extract @20:58:49.44 Lysis1.seek 600uL,nil @20:58:55.31 Archive syringe pumping up to 6 strokes to reach 25psi @20:58:55.32 Archive stroke #1 of 6 @20:58:55.40 Archive.in :pressurize @20:58:55.48 Archive.seek :pressurized @20:59:08.26 15.4psi after pressurize @20:59:08.28 Archive.in :compress @20:59:08.35 Archive.seek :empty @20:59:13.58 Archive.in :default @20:59:13.63 Archive.hold @20:59:13.88 Initial 600uL lysate @16.8psi @20:59:13.89 Handoff.select :blocked @20:59:13.98 Initial air pressure @17.1psi @20:59:14.05 Lysis1.seek 825uL,nil @20:59:16.35 Archive syringe pumping up to 6 strokes to reach 17psi @20:59:16.36 Archive stroke #1 of 6 @20:59:16.49 Archive.in :compress @20:59:16.56 Archive.seek :empty @20:59:19.90 Archive.in :default @20:59:19.95 Archive.hold @20:59:20.20 Recovered 825uL lysate @16.5psi @20:59:20.22 Air.in :halfSpeed @20:59:20.29 Air.seek :fullVent @20:59:22.53 Puck Top Vented, bottom @13.4psi @20:59:22.54 Handoff.select :waste @20:59:22.66 delay 5 seconds @20:59:27.72 Handoff open to waste, bottom @0.0psi @20:59:27.74 Air.in :default @20:59:27.80 Air.seek :evac @20:59:29.25 Lysis2.in :DAmix @20:59:29.35 Lysis1.in :DAmix @20:59:29.87 Lysis2.seek 1480uL @20:59:29.91 Lysis1.seek 575uL @21:00:04.96 delay 5 seconds @21:00:09.98 Handoff.select SPR @21:00:10.11 SPR.command \"start 2 14\" @21:00:11.48 SPR stabilized @21:00:11.60 delay 10 seconds @21:00:21.62 SPR.command \"start 2 0\" @21:00:24.32 Waiting up to 11:00 for SPR to enter state 1 @21:02:21.99 SPR.command \"hold_on\" @21:02:23.90 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @21:02:24.32 Lysis2.seek 1180uL @21:02:24.38 Lysis1.seek 275uL @21:03:06.35 delay 15 seconds @21:03:21.37 SPR.command \"hold_off\" @21:03:24.04 Waiting up to 38:00 for SPR to enter state 7 @21:27:20.23 SPR analysis completed @21:27:24.46 Ended log spr21aug0720h.spr.bz2 @21:27:24.47 SPR.command \"stats_off\" @21:27:26.00 -> Cmd.stop @21:27:26.09 Handoff.select :close @21:27:26.22 Gate.power :heat,:OFF @21:27:26.24 Gate.power :cartridge,:OFF @21:27:26.32 Carousel.seek 41 @21:27:26.50 Plunge.seek :locked @21:27:27.38 PinPort.in :unlocking @21:27:27.44 PinPort.seek :clear @21:27:27.98 Archive.in :unlocking @21:27:28.10 Archive.seek :clear @21:27:28.17 Air.in :unlocking @21:27:28.29 Air.seek :clear @21:27:28.33 Lysis1.in :unlocking @21:27:28.47 Lysis1.seek :clear @21:27:28.56 Lysis2.in :unlocking @21:27:28.63 Lysis2.seek :clear @21:27:32.19 Gate.power :motor,:OFF @21:27:32.21 Gate.power :core,:OFF @21:27:32.23 Gate.power :analytic1,:OFF @21:27:32.25 Gate.power :analytic2,:OFF @21:27:32.27 Gate.power :analytic3,:OFF @21:27:32.30 Gate.power :main,:OFF @21:27:32.32 Safely stopped and ready to power off ##-- file closed 2021-08-08T18:18:50.974Z