##-- file opened 2021-08-06T17:23:31.061Z @13:23:31.34 -> showStatus @13:23:32.95 -> Cmd.loadCartridge -4 @13:23:33.02 Gate.power :main,:ON,limit to 5000mA @13:23:33.09 Gate.power :analytic1,:ON,limit to 5000mA @13:23:33.14 Gate.power :analytic2,:ON,limit to 5000mA @13:23:33.16 Gate.power :analytic3,:ON,limit to 5000mA @13:23:33.29 Powering core bus @13:23:35.58 SPR Module 1, FW Rev. B, FPGA Rev. C @13:23:36.03 Archive.configure :default @13:23:36.15 Air.configure :default @13:23:36.25 Lysis1.configure :default @13:23:36.36 Lysis2.configure :default @13:23:36.48 Plunge.configure :default @13:23:36.60 SamplePump.configure :default @13:23:36.75 ExtraPump.configure :default @13:23:36.85 Toroid.configure :default @13:23:36.98 Carousel.configure :default @13:23:37.11 Pressure.configure :default @13:23:37.23 PinPort.configure :default @13:23:37.29 All elf controllers running firmware version 4.741 @13:23:37.32 Gate.power :motor,:ON,limit to 5000mA @13:23:37.50 Archive.in :default @13:23:37.79 Archive.home.jog 0 @13:23:37.95 Toroid.in :default @13:23:38.03 Plunge.in :default @13:23:38.06 Lysis1.in :default @13:23:38.13 PinPort.in :default @13:23:38.16 Lysis2.in :default @13:23:38.21 Air.in :default @13:23:38.78 Toroid.home.jog 0 @13:23:38.82 Plunge.home.jog 0 @13:23:38.84 Lysis1.home.jog 0 @13:23:38.87 PinPort.home.jog 0 @13:23:38.90 Air.home.jog 0 @13:23:38.94 Lysis2.home.jog 0 @13:23:39.35 Archive.in :homing @13:23:39.46 PinPort.in :homing @13:23:39.54 Toroid.in :homing @13:23:39.62 Archive.jog 1401 @13:23:39.67 Plunge.in :homing @13:23:39.70 Lysis1.in :homing @13:23:39.85 Toroid.jog 627 @13:23:39.92 PinPort.jog -108 @13:23:39.95 Lysis1.jog 1411 @13:23:39.98 Plunge.jog 1562 @13:23:40.20 Lysis2.in :homing @13:23:40.22 Air.in :homing @13:23:40.32 Lysis2.jog 1410 @13:23:40.34 Air.jog 1412 @13:23:40.46 PinPort.setRawPosition! 0 @13:23:40.50 PinPort.seek :home @13:23:40.59 PinPort.in :default @13:23:40.94 Archive.setRawPosition! 50 @13:23:40.99 Archive.seek :home @13:23:41.21 Lysis1.setRawPosition! 50 @13:23:41.26 Lysis1.seek :home @13:23:41.36 Plunge.setRawPosition! 50 @13:23:41.38 Archive.in :default @13:23:41.45 Plunge.seek :home @13:23:41.59 Lysis2.setRawPosition! 50 @13:23:41.61 Lysis1.in :default @13:23:41.64 Air.setRawPosition! 50 @13:23:41.74 Lysis2.seek :home @13:23:41.81 Air.seek :home @13:23:41.95 Plunge.in :default @13:23:42.15 Air.in :default @13:23:42.22 Toroid.setRawPosition! 0 @13:23:42.25 Lysis2.in :default @13:23:42.33 Toroid.seek :home @13:23:42.44 Toroid.in :default @13:23:42.69 Toroid.seek :clear @13:23:44.31 Carousel.in :default @13:23:44.37 Carousel.home.jog 0 @13:23:44.86 Carousel.in :default @13:23:44.92 Carousel.seek 51 @13:23:45.08 Selecting Cartridge 50 @13:23:45.33 Carousel.seek 50 @13:23:45.81 Lysis1.in :fast @13:23:45.87 Lysis1.seek :locked @13:23:48.46 Plunge.seek :locked @13:23:48.59 Air.in :fast @13:23:48.65 Air.seek :locked @13:23:51.27 Powering cartridge bus @13:23:53.89 Gate.setIR 1750,5000,0,6 @13:23:54.02 Gate.setIR 0,5000,0,6 @13:23:54.07 Gate.power :cartridge,:OFF @13:23:54.61 Mounted archive Cartridge @13:24:12.54 -> Cmd.startFiltering @13:24:12.99 Priming sample loop w/100ml, bypass w/10ml @13:24:13.01 SamplePump.setPosition! 0ml @13:24:13.06 Bypass.open @13:24:14.55 Intake.open @13:24:15.35 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:24:17.91 Vacuum tare is 50% of full scale @13:24:18.04 Pressure.in :default @13:24:18.10 Pressure.hold @13:24:18.26 SamplePump.in :prime @13:24:18.33 SamplePump.seek 10ml,59 seconds @13:24:27.59 Pressure.stop @13:24:27.64 SamplePump.setPosition! 0ml @13:24:27.69 Exhaust.open @13:24:29.14 Bypass.close @13:24:30.70 Pressure.in :default @13:24:30.76 Pressure.hold @13:24:30.98 SamplePump.in :prime @13:24:31.05 SamplePump.seek 100ml,5:16 @13:26:34.49 Pressure.stop @13:26:34.55 Bypass.open @13:26:35.26 Intake.close @13:26:36.08 Toroid.in :hold @13:26:36.14 Toroid.seek :crack @13:26:37.65 Toroid.seek :cartridge @13:26:38.73 Toroid.in :default @13:26:38.79 Toroid.seek :clear @13:26:40.75 Pressurizing cartridge to 26psi above 14.8psia @13:26:40.76 SamplePump.setPosition! 0ml @13:26:40.80 Intake.open @13:26:41.55 Bypass.close @13:26:42.26 Exhaust.close @13:26:43.07 Pressure.in :default @13:26:43.13 Pressure.hold @13:26:43.30 SamplePump.in :charge @13:26:43.37 SamplePump.seek 2.3ml,42 seconds @13:26:49.53 Pressure.stop @13:26:49.59 Intake.close @13:26:50.42 Pressurized cartridge with 0.167ml to 26.1psi @13:26:50.44 Isolated @34.9psia (20.1psi) @13:26:50.45 Verifying cartridge holds at least 0.75..0.85 of 34.9psia @13:26:50.49 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:26:55.11 Sampling 1000.0ml @13:26:55.13 SamplePump.setPosition! 0ml @13:26:55.18 Exhaust.open @13:26:55.88 Intake.open @13:26:56.76 Pressure.in :default @13:26:56.82 Pressure.hold @13:26:57.04 SamplePump.in :sample @13:26:57.11 SamplePump.seek 1000ml,1:23:53 @13:26:59.79 Cartridge 50 filtering 1/1000ml at 0.41ml/s with 26.0psi vacuum @13:27:59.79 Cartridge 50 filtering 24/1000ml at 0.40ml/s with 25.6psi vacuum @13:28:59.80 Cartridge 50 filtering 46/1000ml at 0.33ml/s with 25.5psi vacuum @13:29:59.79 Cartridge 50 filtering 68/1000ml at 0.33ml/s with 19.6psi vacuum @13:30:59.80 Cartridge 50 filtering 88/1000ml at 0.31ml/s with 25.5psi vacuum @13:32:00.20 Cartridge 50 filtering 109/1000ml at 0.29ml/s with 25.6psi vacuum @13:32:59.80 Cartridge 50 filtering 128/1000ml at 0.34ml/s with 25.7psi vacuum @13:33:59.79 Cartridge 50 filtering 146/1000ml at 0.30ml/s with 23.5psi vacuum @13:34:59.80 Cartridge 50 filtering 163/1000ml at 0.23ml/s with 24.5psi vacuum @13:35:59.79 Cartridge 50 filtering 179/1000ml at 0.28ml/s with 24.4psi vacuum @13:36:59.80 Cartridge 50 filtering 194/1000ml at 0.28ml/s with 24.9psi vacuum @13:37:59.79 Cartridge 50 filtering 209/1000ml at 0.21ml/s with 21.2psi vacuum @13:38:59.80 Cartridge 50 filtering 223/1000ml at 0.22ml/s with 22.8psi vacuum @13:39:59.79 Cartridge 50 filtering 236/1000ml at 0.24ml/s with 22.4psi vacuum @13:40:59.80 Cartridge 50 filtering 248/1000ml at 0.20ml/s with 24.7psi vacuum @13:41:59.79 Cartridge 50 filtering 261/1000ml at 0.20ml/s with 25.5psi vacuum @13:42:23.16 Flow averaged 0.29ml/s over 15:27 @13:42:23.19 Waiting up to 16 seconds for vacuum to reduce from 29.4 to 18.0psi @13:42:23.75 Relieved excess vacuum in .6 seconds @13:42:23.78 SamplePump.seek 1000ml,1:01:44 @13:42:43.33 Flow averaged 0.20ml/s over 20 seconds @13:42:43.36 Waiting up to 19 seconds for vacuum to reduce from 30.2 to 18.0psi @13:42:43.92 Relieved excess vacuum in .6 seconds @13:42:43.95 SamplePump.seek 1000ml,1:01:24 @13:42:53.18 Flow averaged 0.20ml/s over 10 seconds @13:42:53.20 Intake.close @13:42:53.27 Exhaust.close @13:42:53.43 Toroid.in :hold @13:42:53.50 Toroid.seek :bypass @13:42:55.13 Toroid.in :default @13:42:55.19 Toroid.seek :clear @13:42:56.81 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @13:42:56.82 Pressure.stop @13:42:57.00 Sampled 271.5ml @13:42:58.94 -> Cmd.startProcessing @13:42:59.31 Plunge.seek :reagent @13:43:00.14 Sample loop closed. Ready to Deliver Reagents @13:43:00.15 Archive.seek :sealed @13:43:04.42 Archive.seek :evac @13:43:18.36 delay 1:00 @13:44:18.38 Archive.seek :clear @13:44:36.09 RNAlater soak for 10:00 @13:54:36.11 Archive.seek :tVent @13:54:38.69 delay 1 second @13:54:39.71 Archive.seek :evac @13:54:55.33 delay 10 seconds @13:55:05.36 Archive.seek :tVent @13:55:20.98 delay 1 second @13:55:22.00 Archive.seek :evac @13:55:37.62 delay 10 seconds @13:55:47.65 Sample preserved and evacuated @13:55:48.61 -> Cmd.stop @13:55:48.75 Carousel.seek 50 @13:55:48.92 Plunge.seek :locked @13:55:49.87 Archive.in :unlocking @13:55:50.00 Archive.seek :clear @13:55:50.08 Air.in :unlocking @13:55:50.19 Lysis1.in :unlocking @13:55:50.23 Air.seek :clear @13:55:50.39 Lysis1.seek :clear @13:55:54.17 Gate.power :motor,:OFF @13:55:54.19 Gate.power :core,:OFF @13:55:54.21 Gate.power :analytic1,:OFF @13:55:54.23 Gate.power :analytic2,:OFF @13:55:54.25 Gate.power :analytic3,:OFF @13:55:54.27 Gate.power :main,:OFF @13:55:54.29 Safely stopped and ready to power off ##-- file closed 2021-08-06T18:04:52.098Z ##-- file opened 2021-08-06T18:04:52.101Z @14:04:52.65 -> showStatus @14:04:54.66 -> Cmd.loadCartridge -18 @14:04:54.72 Gate.power :main,:ON,limit to 5000mA @14:04:54.74 Gate.power :analytic1,:ON,limit to 5000mA @14:04:54.76 Gate.power :analytic2,:ON,limit to 5000mA @14:04:54.79 Gate.power :analytic3,:ON,limit to 5000mA @14:04:54.92 Powering core bus @14:04:57.21 SPR Module 1, FW Rev. B, FPGA Rev. C @14:04:57.67 Archive.configure :default @14:04:57.79 Air.configure :default @14:04:57.89 Lysis1.configure :default @14:04:58.00 Lysis2.configure :default @14:04:58.12 Plunge.configure :default @14:04:58.24 SamplePump.configure :default @14:04:58.39 ExtraPump.configure :default @14:04:58.49 Toroid.configure :default @14:04:58.62 Carousel.configure :default @14:04:58.75 Pressure.configure :default @14:04:58.86 PinPort.configure :default @14:04:58.93 All elf controllers running firmware version 4.741 @14:04:58.96 Gate.power :motor,:ON,limit to 5000mA @14:04:59.08 Archive.in :default @14:04:59.42 Archive.home.jog 0 @14:04:59.59 PinPort.in :default @14:04:59.65 Toroid.in :default @14:04:59.69 Lysis2.in :default @14:04:59.72 Air.in :default @14:04:59.76 Lysis1.in :default @14:05:00.19 Plunge.in :default @14:05:00.38 PinPort.home.jog 0 @14:05:00.40 Toroid.home.jog 0 @14:05:00.44 Lysis2.home.jog 0 @14:05:00.46 Air.home.jog 0 @14:05:00.49 Lysis1.home.jog 0 @14:05:00.53 Plunge.home.jog 0 @14:05:00.97 Archive.in :homing @14:05:01.19 Toroid.in :homing @14:05:01.23 Plunge.in :homing @14:05:01.26 Air.in :homing @14:05:01.30 PinPort.in :homing @14:05:01.33 Lysis1.in :homing @14:05:01.37 Archive.jog 1400 @14:05:01.42 Lysis2.in :homing @14:05:01.57 Toroid.jog 623 @14:05:01.62 Plunge.jog 1569 @14:05:01.74 PinPort.jog -108 @14:05:01.80 Lysis2.jog 1412 @14:05:01.82 Air.jog 1407 @14:05:01.84 Lysis1.jog 1413 @14:05:02.09 PinPort.setRawPosition! 0 @14:05:02.13 PinPort.seek :home @14:05:02.22 PinPort.in :default @14:05:02.76 Archive.setRawPosition! 50 @14:05:02.80 Archive.seek :home @14:05:02.93 Plunge.setRawPosition! 50 @14:05:02.98 Plunge.seek :home @14:05:03.11 Lysis2.setRawPosition! 50 @14:05:03.14 Lysis1.setRawPosition! 50 @14:05:03.16 Air.setRawPosition! 50 @14:05:03.19 Archive.in :default @14:05:03.31 Lysis2.seek :home @14:05:03.44 Air.seek :home @14:05:03.47 Lysis1.seek :home @14:05:03.61 Plunge.in :default @14:05:03.70 Lysis2.in :default @14:05:03.82 Toroid.setRawPosition! 0 @14:05:03.84 Lysis1.in :default @14:05:03.87 Air.in :default @14:05:03.97 Toroid.seek :home @14:05:04.12 Toroid.in :default @14:05:04.37 Toroid.seek :clear @14:05:05.99 Carousel.in :default @14:05:06.06 Carousel.home.jog 0 @14:05:06.54 Carousel.in :default @14:05:06.60 Carousel.seek 50 @14:05:06.75 Selecting Cartridge 49 @14:05:07.00 Carousel.seek 49 @14:05:07.48 Lysis1.in :fast @14:05:07.54 Lysis1.seek :locked @14:05:10.13 Plunge.seek :locked @14:05:10.25 Air.in :fast @14:05:10.31 Air.seek :locked @14:05:12.91 Powering cartridge bus @14:05:15.54 Gate.setIR 1750,5000,0,6 @14:05:15.67 Gate.setIR 0,5000,0,6 @14:05:15.73 PuckHeater.configure :default @14:05:15.79 Gate.power :heat,:ON,limit to 5000mA @14:05:16.45 Mounted lysis Cartridge board#Q05053 @14:05:16.48 Using default puck heat loss curve for HeatingAccelInCarousel @14:05:35.06 -> Cmd.startFiltering @14:05:35.51 Priming sample loop w/100ml, bypass w/10ml @14:05:35.53 SamplePump.setPosition! 0ml @14:05:35.58 Bypass.open @14:05:37.03 Intake.open @14:05:37.80 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:05:40.37 Vacuum tare is 51% of full scale @14:05:40.49 Pressure.in :default @14:05:40.55 Pressure.hold @14:05:40.72 SamplePump.in :prime @14:05:40.79 SamplePump.seek 10ml,59 seconds @14:05:50.04 Pressure.stop @14:05:50.09 SamplePump.setPosition! 0ml @14:05:50.14 Exhaust.open @14:05:51.54 Bypass.close @14:05:53.04 Pressure.in :default @14:05:53.10 Pressure.hold @14:05:53.31 SamplePump.in :prime @14:05:53.38 SamplePump.seek 100ml,5:16 @14:07:56.77 Pressure.stop @14:07:56.83 Bypass.open @14:07:57.51 Intake.close @14:07:58.32 Toroid.in :hold @14:07:58.38 Toroid.seek :crack @14:07:59.89 Toroid.seek :cartridge @14:08:00.98 Toroid.in :default @14:08:01.04 Toroid.seek :clear @14:08:02.99 Pressurizing cartridge to 14psi above 14.6psia @14:08:03.01 SamplePump.setPosition! 0ml @14:08:03.05 Intake.open @14:08:03.77 Bypass.close @14:08:04.47 Exhaust.close @14:08:05.28 Pressure.in :default @14:08:05.34 Pressure.hold @14:08:05.51 SamplePump.in :charge @14:08:05.58 SamplePump.seek 2.3ml,41 seconds @14:08:14.05 Pressure.stop @14:08:14.10 Intake.close @14:08:14.90 Pressurized cartridge with 0.318ml to 14.5psi @14:08:14.91 Isolated @23.8psia (9.3psi) @14:08:14.93 Verifying cartridge holds at least 0.75..0.85 of 23.8psia @14:08:14.96 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:08:19.58 Sampling 75.0ml @14:08:19.60 SamplePump.setPosition! 0ml @14:08:19.65 Exhaust.open @14:08:20.37 Intake.open @14:08:20.70 Pressure.in :default @14:08:20.76 Pressure.hold @14:08:20.98 SamplePump.in :sample @14:08:21.05 SamplePump.seek 75ml,5:31 @14:08:23.73 Cartridge 49 filtering 1/75ml at 0.35ml/s with 11.8psi vacuum @14:09:23.73 Cartridge 49 filtering 21/75ml at 0.33ml/s with 11.4psi vacuum @14:10:24.15 Cartridge 49 filtering 40/75ml at 0.34ml/s with 11.4psi vacuum @14:11:23.74 Cartridge 49 filtering 59/75ml at 0.33ml/s with 12.5psi vacuum @14:12:15.41 Pressure.stop @14:12:15.53 Intake.close @14:12:15.58 Exhaust.close @14:12:15.74 Toroid.in :hold @14:12:15.80 Toroid.seek :bypass @14:12:17.42 Toroid.in :default @14:12:17.48 Toroid.seek :clear @14:12:19.10 Sampled 75.0ml @14:12:20.69 -> Cmd.startProcessing @14:12:22.20 SPR.command \"read_sequences\" @14:12:22.57 SPR.command \"stats_on\" @14:12:22.61 SPR.command \"start 0 1\" @14:12:23.42 SPR.start /var/log/esp/spr21aug0614h.spr.bz2 @14:12:25.42 Handoff.select :waste @14:12:32.10 Syringe tare is 19% of full scale @14:12:32.12 PinPort.in :locking @14:12:32.19 PinPort.seek :locked @14:12:32.68 Archive.in :fast @14:12:32.76 Lysis2.in :fast @14:12:32.80 Lysis1.seek 1350uL @14:12:32.84 Archive.seek :full @14:12:32.96 Lysis2.seek :full @14:12:35.03 Plunge.seek :reagent @14:12:35.93 Evacuating puck with filter bubble point of 24psi @14:12:35.95 PinPort.seek :locked @14:12:36.03 Handoff.select :waste @14:12:42.62 Air syringe pumping up to 1 stroke to reach 8psi @14:12:42.63 Air stroke #1 of 1 @14:12:42.72 Air.in :pressurize @14:12:42.78 Air.seek :pressurized @14:12:54.03 0.5psi after pressurize @14:12:54.04 Air.in :compress @14:12:54.13 Air.seek :evac @14:12:58.22 0.7psi after compress @14:13:10.79 0.7psi after compress (stable) @14:13:10.81 Air.in :default @14:13:10.87 Air.hold @14:13:11.04 at 0.6psi Opened to waste @14:13:11.05 Handoff.select :blocked @14:13:11.18 Archive syringe pumping up to 8 strokes to reach 24psi @14:13:11.19 Archive stroke #1 of 8 @14:13:11.27 Archive.in :pressurize @14:13:11.37 Archive.seek :pressurized @14:13:24.12 1.7psi after pressurize @14:13:24.13 Archive.in :compress @14:13:24.21 Archive.seek :empty @14:13:40.09 16.2psi after compress @14:13:52.67 17.4psi after compress (stable) @14:13:52.68 Archive.in :gulp @14:13:52.75 Archive.seek :topVent @14:13:59.96 17.4psi after gulp @14:14:12.53 17.4psi after gulp (stable) @14:14:12.54 Archive stroke #2 of 8 @14:14:13.07 Archive.in :pressurize @14:14:13.15 Archive.seek :pressurized @14:14:25.88 17.3psi after pressurize @14:14:25.89 Archive.in :compress @14:14:25.97 Archive.seek :empty @14:14:37.35 Reached 24.2 of 24.0psi after pumping 3364uL (1.94 strokes) @14:14:37.38 Archive.in :default @14:14:37.46 Archive.hold @14:14:37.53 Handoff.select :waste @14:14:46.72 delay 1:00 @14:15:46.74 Handoff.select :blocked @14:16:09.42 Handoff.select :waste @14:16:09.54 0.3psi at evacEnd @14:16:09.55 Handoff.select :blocked @14:16:09.69 Archive.in :fast @14:16:09.75 Archive.seek :topVent @14:16:23.12 Stable @0.4psi after Handoff blocked @14:16:23.14 Archive syringe pumping up to 8 strokes to reach 15psi @14:16:23.16 Archive stroke #1 of 8 @14:16:23.25 Archive.in :pressurize @14:16:23.32 Archive.seek :pressurized @14:16:36.06 0.5psi after pressurize @14:16:36.07 Archive.in :compress @14:16:36.15 Archive.seek :empty @14:16:52.03 14.9psi after compress @14:16:52.95 Reached 15.4 of 15.4psi after pumping 1730uL (1.00 strokes) @14:16:52.96 Archive.in :default @14:16:53.03 Archive.hold @14:16:53.12 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position @14:16:53.14 Handoff.select :waste @14:17:00.33 delay 1:00 @14:18:00.36 Handoff.select :blocked @14:18:23.04 Handoff.select :waste @14:18:23.16 -0.0psi at evacEnd @14:18:23.18 Air.seek :fullVent @14:18:24.62 Archive.in :fast @14:18:24.68 Archive.seek :topVent @14:18:31.82 Seawater evacuated, Presure above/below filter released. Lysis begins @14:18:31.91 Lysis1.in :slow @14:18:31.99 Lysis1.seek 1050uL @14:19:02.01 delay 5 seconds @14:19:07.03 Handoff.select :blocked @14:19:07.20 Lysis1.seek 450uL @14:20:07.13 delay 3 seconds @14:20:10.20 Air.in to mix into 1.37806 parts Lysis1 @14:20:10.72 Air.seek :evac @14:20:10.76 Lysis1.seek 300uL @14:20:25.86 Lysis1.seek :empty @14:20:55.95 Lysis buffer delivered @16.7psi @14:20:55.96 Polling Can every 2:00 @14:20:56.51 PuckHeater.configure :default #ambient 24.4C @14:20:56.55 PuckHeater.seek 79.6C @14:21:45.01 PuckHeater.hold 2:00,79.6C @14:23:45.06 Polling Can every 2:00 @14:23:45.61 PuckHeater.configure :default #ambient 24.4C @14:23:45.66 PuckHeater.hold 30 seconds,78.6C @14:24:15.82 Polling Can every 2:00 @14:24:16.36 PuckHeater.configure :default #ambient 24.4C @14:24:16.41 PuckHeater.hold 30 seconds,78.1C @14:24:46.58 Polling Can every 2:00 @14:24:47.13 PuckHeater.configure :default #ambient 24.4C @14:24:47.18 PuckHeater.hold 10:00,77.6C @14:34:46.49 Lysis complete. Syringe pressure @17.0psi @14:34:46.50 Handoff.select :blocked @14:34:46.55 Archive syringe pumping up to 5 strokes to reach 40psi Archive stroke #1 of 5 @14:34:46.64 Archive.in :pressurize @14:34:46.70 Archive.seek :pressurized @14:34:59.43 16.8psi after pressurize @14:34:59.46 Archive.in :compress @14:34:59.53 Archive.seek :empty @14:35:09.27 Reached 41.0 of 40.0psi after pumping 1599uL (0.92 strokes) @14:35:09.29 Archive.in :default @14:35:09.36 Archive.hold @14:35:09.43 delay 2 seconds @14:35:11.45 Archive.in :fast @14:35:11.52 Archive.seek :full @14:35:18.26 Lysis1.seek 138uL @14:35:32.12 delay 1 second @14:35:33.15 Archive.seek :full @14:35:33.17 Lysis1.seek :empty @14:35:47.14 Initial air pressure @59.1psi @14:35:47.59 Lysis1.in :extract @14:35:47.70 Lysis1.seek 600uL,nil @14:35:51.14 Archive syringe pumping up to 6 strokes to reach 25psi @14:35:51.15 Archive stroke #1 of 6 @14:35:51.24 Archive.in :pressurize @14:35:51.32 Archive.seek :pressurized @14:36:04.07 15.8psi after pressurize @14:36:04.09 Archive.in :compress @14:36:04.17 Archive.seek :empty @14:36:13.27 Syringe::LysatePlungerStuck in PROCESSING -- at < 302uL during Lysis Syringe #1 (LyS1) move @14:36:13.30 Lysis1.in :default @14:36:13.37 Lysis1.seek 267uL @14:36:14.00 Reached 28.1 of 25.0psi after pumping 1601uL (0.93 strokes) @14:36:14.01 Archive.in :default @14:36:14.08 Archive.hold @14:36:15.18 Lysis1.in :extract @14:36:15.25 Lysis1.seek 600uL,nil @14:36:19.92 Archive syringe pumping up to 6 strokes to reach 25psi @14:36:19.93 Archive stroke #1 of 6 @14:36:20.02 Archive.in :compress @14:36:20.09 Archive.seek :empty @14:36:21.35 Archive.in :default @14:36:21.40 Archive.hold @14:36:21.64 Initial 600uL lysate @20.3psi @14:36:21.65 Handoff.select :blocked @14:36:21.77 Initial air pressure @20.8psi @14:36:21.85 Lysis1.seek 825uL,nil @14:36:23.05 Archive syringe pumping up to 6 strokes to reach 17psi @14:36:23.06 Archive stroke #1 of 6 @14:36:23.15 Archive.in :compress @14:36:23.21 Archive.seek :empty @14:36:27.55 Archive.in :default @14:36:27.60 Archive.hold @14:36:27.87 Recovered 825uL lysate @18.3psi @14:36:27.89 Air.in :halfSpeed @14:36:27.96 Air.seek :fullVent @14:36:30.20 Puck Top Vented, bottom @3.3psi @14:36:30.22 Handoff.select :waste @14:36:30.35 delay 5 seconds @14:36:35.41 Handoff open to waste, bottom @-0.1psi @14:36:35.43 Air.in :default @14:36:35.49 Air.seek :evac @14:36:36.93 Lysis2.in :DAmix @14:36:37.03 Lysis1.in :DAmix @14:36:37.61 Lysis2.seek 1480uL @14:36:37.66 Lysis1.seek 575uL @14:37:12.69 delay 5 seconds @14:37:17.71 Handoff.select SPR @14:37:17.84 SPR.command \"start 2 14\" @14:37:18.92 SPR stabilized @14:37:19.05 delay 10 seconds @14:37:29.07 SPR.command \"start 2 0\" @14:37:32.28 Waiting up to 11:00 for SPR to enter state 1 @14:39:29.95 SPR.command \"hold_on\" @14:39:31.34 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @14:39:31.79 Lysis2.seek 1180uL @14:39:31.83 Lysis1.seek 275uL @14:40:13.85 delay 15 seconds @14:40:28.87 SPR.command \"hold_off\" @14:40:31.99 Waiting up to 38:00 for SPR to enter state 7 @15:04:29.14 SPR analysis completed @15:04:33.22 Ended log spr21aug0614h.spr.bz2 @15:04:33.23 SPR.command \"stats_off\" @15:04:34.63 -> Cmd.stop @15:04:34.64 Archive.stop @15:04:34.76 Handoff.select :close @15:04:34.89 Gate.power :heat,:OFF @15:04:34.91 Gate.power :cartridge,:OFF @15:04:34.99 Carousel.seek 49 @15:04:35.17 Plunge.seek :locked @15:04:36.05 PinPort.in :unlocking @15:04:36.11 PinPort.seek :clear @15:04:36.66 Archive.in :unlocking @15:04:36.79 Archive.seek :clear @15:04:36.82 Air.in :unlocking @15:04:36.97 Air.seek :clear @15:04:37.01 Lysis1.in :unlocking @15:04:37.17 Lysis1.seek :clear @15:04:37.24 Lysis2.in :unlocking @15:04:37.31 Lysis2.seek :clear @15:04:40.98 Gate.power :motor,:OFF @15:04:41.00 Gate.power :core,:OFF @15:04:41.02 Gate.power :analytic1,:OFF @15:04:41.04 Gate.power :analytic2,:OFF @15:04:41.06 Gate.power :analytic3,:OFF @15:04:41.08 Gate.power :main,:OFF @15:04:41.10 Safely stopped and ready to power off ##-- file closed 2021-08-06T22:58:58.818Z ##-- file opened 2021-08-06T22:58:58.820Z @18:58:59.63 -> showStatus @18:59:01.66 -> Cmd.loadCartridge -4 @18:59:01.72 Gate.power :main,:ON,limit to 5000mA @18:59:01.76 Gate.power :analytic1,:ON,limit to 5000mA @18:59:01.78 Gate.power :analytic2,:ON,limit to 5000mA @18:59:01.80 Gate.power :analytic3,:ON,limit to 5000mA @18:59:01.94 Powering core bus @18:59:04.22 SPR Module 1, FW Rev. B, FPGA Rev. C @18:59:04.72 Archive.configure :default @18:59:04.84 Air.configure :default @18:59:04.94 Lysis1.configure :default @18:59:05.05 Lysis2.configure :default @18:59:05.17 Plunge.configure :default @18:59:05.30 SamplePump.configure :default @18:59:05.44 ExtraPump.configure :default @18:59:05.56 Toroid.configure :default @18:59:05.68 Carousel.configure :default @18:59:05.81 Pressure.configure :default @18:59:05.92 PinPort.configure :default @18:59:05.99 All elf controllers running firmware version 4.741 @18:59:06.02 Gate.power :motor,:ON,limit to 5000mA @18:59:06.18 Archive.in :default @18:59:06.51 Archive.home.jog 0 @18:59:06.66 Toroid.in :default @18:59:07.09 Plunge.in :default @18:59:07.15 Lysis1.in :default @18:59:07.18 PinPort.in :default @18:59:07.24 Air.in :default @18:59:07.30 Lysis2.in :default @18:59:07.47 Toroid.home.jog 0 @18:59:07.52 Plunge.home.jog 0 @18:59:07.55 Lysis1.home.jog 0 @18:59:07.57 PinPort.home.jog 0 @18:59:07.60 Lysis2.home.jog 0 @18:59:07.65 Air.home.jog 0 @18:59:08.10 Archive.in :homing @18:59:08.32 Toroid.in :homing @18:59:08.36 Plunge.in :homing @18:59:08.39 Air.in :homing @18:59:08.42 Archive.jog 1399 @18:59:08.46 PinPort.in :homing @18:59:08.49 Lysis1.in :homing @18:59:08.53 Lysis2.in :homing @18:59:08.75 Toroid.jog 629 @18:59:08.78 Plunge.jog 1567 @18:59:08.81 PinPort.jog -108 @18:59:08.83 Air.jog 1415 @18:59:08.87 Lysis1.jog 1412 @18:59:08.91 Lysis2.jog 1410 @18:59:09.27 PinPort.setRawPosition! 0 @18:59:09.32 PinPort.seek :home @18:59:09.40 PinPort.in :default @18:59:09.76 Archive.setRawPosition! 50 @18:59:09.81 Archive.seek :home @18:59:10.08 Archive.in :default @18:59:10.17 Plunge.setRawPosition! 50 @18:59:10.25 Air.setRawPosition! 50 @18:59:10.27 Plunge.seek :home @18:59:10.37 Air.seek :home @18:59:10.45 Lysis2.setRawPosition! 50 @18:59:10.47 Lysis1.setRawPosition! 50 @18:59:10.54 Lysis2.seek :home @18:59:10.59 Lysis1.seek :home @18:59:10.74 Air.in :default @18:59:10.76 Plunge.in :default @18:59:10.90 Lysis2.in :default @18:59:10.99 Lysis1.in :default @18:59:11.07 Toroid.setRawPosition! 0 @18:59:11.13 Toroid.seek :home @18:59:11.22 Toroid.in :default @18:59:11.40 Toroid.seek :clear @18:59:13.02 Carousel.in :default @18:59:13.08 Carousel.home.jog 0 @18:59:13.58 Carousel.in :default @18:59:13.64 Carousel.seek 49 @18:59:13.81 Selecting Cartridge 48 @18:59:14.06 Carousel.seek 48 @18:59:14.53 Lysis1.in :fast @18:59:14.59 Lysis1.seek :locked @18:59:17.19 Plunge.seek :locked @18:59:17.32 Air.in :fast @18:59:17.39 Air.seek :locked @18:59:19.99 Powering cartridge bus @18:59:22.61 Gate.setIR 1750,5000,0,6 @18:59:22.74 Gate.setIR 0,5000,0,6 @18:59:22.79 Gate.power :cartridge,:OFF @18:59:23.33 Mounted archive Cartridge @18:59:41.24 -> Cmd.startFiltering @18:59:41.70 Priming sample loop w/100ml, bypass w/10ml @18:59:41.72 SamplePump.setPosition! 0ml @18:59:41.77 Bypass.open @18:59:43.24 Intake.open @18:59:43.52 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:59:46.09 Vacuum tare is 50% of full scale @18:59:46.22 Pressure.in :default @18:59:46.29 Pressure.hold @18:59:46.46 SamplePump.in :prime @18:59:46.52 SamplePump.seek 10ml,59 seconds @18:59:55.78 Pressure.stop @18:59:55.83 SamplePump.setPosition! 0ml @18:59:55.88 Exhaust.open @18:59:57.30 Bypass.close @18:59:58.84 Pressure.in :default @18:59:58.90 Pressure.hold @18:59:59.12 SamplePump.in :prime @18:59:59.19 SamplePump.seek 100ml,5:16 @19:02:02.80 Pressure.stop @19:02:02.86 Bypass.open @19:02:03.57 Intake.close @19:02:04.39 Toroid.in :hold @19:02:04.45 Toroid.seek :crack @19:02:05.96 Toroid.seek :cartridge @19:02:07.05 Toroid.in :default @19:02:07.11 Toroid.seek :clear @19:02:09.07 Pressurizing cartridge to 26psi above 15.0psia @19:02:09.08 SamplePump.setPosition! 0ml @19:02:09.12 Intake.open @19:02:09.38 Bypass.close @19:02:10.09 Exhaust.close @19:02:10.89 Pressure.in :default @19:02:10.95 Pressure.hold @19:02:11.11 SamplePump.in :charge @19:02:11.18 SamplePump.seek 2.3ml,42 seconds @19:02:18.26 Pressure.stop @19:02:18.31 Intake.close @19:02:19.11 Pressurized cartridge with 0.221ml to 26.8psi @19:02:19.13 Isolated @37.2psia (22.2psi) @19:02:19.15 Verifying cartridge holds at least 0.75..0.85 of 37.2psia @19:02:19.18 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @19:02:23.75 Cartridge pressure fell 1% to 37.0psia @19:02:23.82 Sampling 1000.0ml @19:02:23.84 SamplePump.setPosition! 0ml @19:02:23.89 Exhaust.open @19:02:24.59 Intake.open @19:02:25.45 Pressure.in :default @19:02:25.50 Pressure.hold @19:02:25.73 SamplePump.in :sample @19:02:25.80 SamplePump.seek 1000ml,1:23:53 @19:02:28.48 Cartridge 48 filtering 1/1000ml at 0.35ml/s with 21.1psi vacuum @19:03:28.47 Cartridge 48 filtering 24/1000ml at 0.41ml/s with 22.0psi vacuum @19:04:28.88 Cartridge 48 filtering 46/1000ml at 0.33ml/s with 23.2psi vacuum @19:05:28.47 Cartridge 48 filtering 68/1000ml at 0.38ml/s with 18.1psi vacuum @19:06:28.48 Cartridge 48 filtering 89/1000ml at 0.37ml/s with 26.2psi vacuum @19:07:28.47 Cartridge 48 filtering 109/1000ml at 0.31ml/s with 21.0psi vacuum @19:08:28.48 Cartridge 48 filtering 128/1000ml at 0.35ml/s with 22.7psi vacuum @19:09:28.47 Cartridge 48 filtering 147/1000ml at 0.31ml/s with 18.6psi vacuum @19:10:28.48 Cartridge 48 filtering 164/1000ml at 0.24ml/s with 24.4psi vacuum @19:11:28.47 Cartridge 48 filtering 180/1000ml at 0.21ml/s with 24.5psi vacuum @19:12:28.48 Cartridge 48 filtering 195/1000ml at 0.24ml/s with 22.4psi vacuum @19:13:28.48 Cartridge 48 filtering 209/1000ml at 0.25ml/s with 26.6psi vacuum @19:14:28.48 Cartridge 48 filtering 222/1000ml at 0.24ml/s with 22.7psi vacuum @19:15:28.48 Cartridge 48 filtering 235/1000ml at 0.20ml/s with 23.9psi vacuum @19:15:42.20 Flow averaged 0.30ml/s over 13:17 @19:15:42.23 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @19:15:42.80 Relieved excess vacuum in .6 seconds @19:15:42.83 SamplePump.seek 1000ml,1:04:04 @19:16:03.43 Flow averaged 0.20ml/s over 21 seconds @19:16:03.46 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi @19:16:04.03 Relieved excess vacuum in .6 seconds @19:16:04.06 SamplePump.seek 1000ml,1:03:43 @19:16:13.54 Flow averaged 0.20ml/s over 10 seconds @19:16:13.56 Intake.close @19:16:13.60 Exhaust.close @19:16:13.79 Toroid.in :hold @19:16:13.85 Toroid.seek :bypass @19:16:15.48 Toroid.in :default @19:16:15.54 Toroid.seek :clear @19:16:17.16 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @19:16:17.17 Pressure.stop @19:16:17.36 Sampled 243.7ml @19:16:19.57 -> Cmd.startProcessing @19:16:19.94 Plunge.seek :reagent @19:16:20.77 Sample loop closed. Ready to Deliver Reagents @19:16:20.78 Archive.seek :sealed @19:16:25.05 Archive.seek :evac @19:16:38.97 delay 1:00 @19:17:38.99 Archive.seek :clear @19:17:56.70 RNAlater soak for 10:00 @19:27:56.72 Archive.seek :tVent @19:27:59.29 delay 1 second @19:28:00.32 Archive.seek :evac @19:28:15.93 delay 10 seconds @19:28:25.97 Archive.seek :tVent @19:28:41.57 delay 1 second @19:28:42.60 Archive.seek :evac @19:28:58.22 delay 10 seconds @19:29:08.26 Sample preserved and evacuated @19:29:09.22 -> Cmd.stop @19:29:09.36 Carousel.seek 48 @19:29:09.53 Plunge.seek :locked @19:29:10.48 Archive.in :unlocking @19:29:10.61 Archive.seek :clear @19:29:10.69 Air.in :unlocking @19:29:10.80 Air.seek :clear @19:29:10.87 Lysis1.in :unlocking @19:29:10.99 Lysis1.seek :clear @19:29:14.78 Gate.power :motor,:OFF @19:29:14.80 Gate.power :core,:OFF @19:29:14.82 Gate.power :analytic1,:OFF @19:29:14.84 Gate.power :analytic2,:OFF @19:29:14.86 Gate.power :analytic3,:OFF @19:29:14.89 Gate.power :main,:OFF @19:29:14.91 Safely stopped and ready to power off ##-- file closed 2021-08-06T23:38:10.982Z ##-- file opened 2021-08-06T23:38:10.987Z @19:38:10.93 -> showStatus @19:38:12.94 -> Cmd.loadCartridge -18 @19:38:13.00 Gate.power :main,:ON,limit to 5000mA @19:38:13.02 Gate.power :analytic1,:ON,limit to 5000mA @19:38:13.04 Gate.power :analytic2,:ON,limit to 5000mA @19:38:13.07 Gate.power :analytic3,:ON,limit to 5000mA @19:38:13.23 Powering core bus @19:38:15.49 SPR Module 1, FW Rev. B, FPGA Rev. C @19:38:15.98 Archive.configure :default @19:38:16.10 Air.configure :default @19:38:16.20 Lysis1.configure :default @19:38:16.31 Lysis2.configure :default @19:38:16.44 Plunge.configure :default @19:38:16.56 SamplePump.configure :default @19:38:16.70 ExtraPump.configure :default @19:38:16.82 Toroid.configure :default @19:38:16.94 Carousel.configure :default @19:38:17.07 Pressure.configure :default @19:38:17.19 PinPort.configure :default @19:38:17.24 All elf controllers running firmware version 4.741 @19:38:17.27 Gate.power :motor,:ON,limit to 5000mA @19:38:17.40 Archive.in :default @19:38:17.65 Archive.home.jog 0 @19:38:17.85 Toroid.in :default @19:38:18.29 Lysis1.in :default @19:38:18.34 Plunge.in :default @19:38:18.40 Lysis2.in :default @19:38:18.44 PinPort.in :default @19:38:18.51 Air.in :default @19:38:18.66 Toroid.home.jog 0 @19:38:18.69 Lysis1.home.jog 0 @19:38:18.72 Plunge.home.jog 0 @19:38:18.75 Lysis2.home.jog 0 @19:38:18.77 PinPort.home.jog 0 @19:38:18.85 Air.home.jog 0 @19:38:19.27 Archive.in :homing @19:38:19.49 Toroid.in :homing @19:38:19.53 Plunge.in :homing @19:38:19.56 Archive.jog 1402 @19:38:19.60 PinPort.in :homing @19:38:19.63 Lysis2.in :homing @19:38:19.66 Lysis1.in :homing @19:38:19.69 Air.in :homing @19:38:19.89 Plunge.jog 1550 @19:38:19.93 Toroid.jog 632 @19:38:19.96 PinPort.jog -109 @19:38:19.99 Lysis2.jog 1410 @19:38:20.02 Air.jog 1410 @19:38:20.05 Lysis1.jog 1414 @19:38:20.38 PinPort.setRawPosition! 0 @19:38:20.43 PinPort.seek :home @19:38:20.51 PinPort.in :default @19:38:20.93 Archive.setRawPosition! 50 @19:38:20.98 Archive.seek :home @19:38:21.28 Plunge.setRawPosition! 50 @19:38:21.31 Lysis2.setRawPosition! 50 @19:38:21.34 Archive.in :default @19:38:21.44 Plunge.seek :home @19:38:21.55 Lysis2.seek :home @19:38:21.66 Lysis1.setRawPosition! 50 @19:38:21.68 Air.setRawPosition! 50 @19:38:21.75 Lysis1.seek :home @19:38:21.82 Air.seek :home @19:38:21.96 Plunge.in :default @19:38:21.98 Lysis2.in :default @19:38:22.13 Lysis1.in :default @19:38:22.22 Air.in :default @19:38:22.30 Toroid.setRawPosition! 0 @19:38:22.35 Toroid.seek :home @19:38:22.45 Toroid.in :default @19:38:22.63 Toroid.seek :clear @19:38:24.25 Carousel.in :default @19:38:24.31 Carousel.home.jog 0 @19:38:24.81 Carousel.in :default @19:38:24.87 Carousel.seek 48 @19:38:25.04 Selecting Cartridge 47 @19:38:25.29 Carousel.seek 47 @19:38:25.77 Lysis1.in :fast @19:38:25.83 Lysis1.seek :locked @19:38:28.42 Plunge.seek :locked @19:38:28.54 Air.in :fast @19:38:28.60 Air.seek :locked @19:38:31.20 Powering cartridge bus @19:38:33.82 Gate.setIR 1750,5000,0,6 @19:38:33.95 Gate.setIR 0,5000,0,6 @19:38:34.01 PuckHeater.configure :default @19:38:34.06 Gate.power :heat,:ON,limit to 5000mA @19:38:34.73 Mounted lysis Cartridge board#Q05080 @19:38:34.76 Using default puck heat loss curve for HeatingAccelInCarousel @19:38:53.34 -> Cmd.startFiltering @19:38:53.80 Priming sample loop w/100ml, bypass w/10ml @19:38:53.81 SamplePump.setPosition! 0ml @19:38:53.85 Bypass.open @19:38:55.33 Intake.open @19:38:56.12 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @19:38:58.69 Vacuum tare is 51% of full scale @19:38:58.88 Pressure.in :default @19:38:58.94 Pressure.hold @19:38:59.11 SamplePump.in :prime @19:38:59.17 SamplePump.seek 10ml,59 seconds @19:39:08.44 Pressure.stop @19:39:08.49 SamplePump.setPosition! 0ml @19:39:08.54 Exhaust.open @19:39:09.94 Bypass.close @19:39:11.48 Pressure.in :default @19:39:11.54 Pressure.hold @19:39:11.75 SamplePump.in :prime @19:39:11.81 SamplePump.seek 100ml,5:16 @19:41:15.30 Pressure.stop @19:41:15.36 Bypass.open @19:41:16.11 Intake.close @19:41:16.93 Toroid.in :hold @19:41:16.99 Toroid.seek :crack @19:41:18.51 Toroid.seek :cartridge @19:41:19.59 Toroid.in :default @19:41:19.65 Toroid.seek :clear @19:41:21.61 Pressurizing cartridge to 14psi above 14.5psia @19:41:21.62 SamplePump.setPosition! 0ml @19:41:21.66 Intake.open @19:41:22.42 Bypass.close @19:41:23.16 Exhaust.close @19:41:23.94 Pressure.in :default @19:41:24.00 Pressure.hold @19:41:24.16 SamplePump.in :charge @19:41:24.23 SamplePump.seek 2.3ml,41 seconds @19:41:34.03 Pressure.stop @19:41:34.08 Intake.close @19:41:34.88 Pressurized cartridge with 0.428ml to 14.5psi @19:41:34.90 Isolated @25.9psia (11.4psi) @19:41:34.91 Verifying cartridge holds at least 0.75..0.85 of 25.9psia @19:41:34.95 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @19:41:39.57 Sampling 75.0ml @19:41:39.58 SamplePump.setPosition! 0ml @19:41:39.64 Exhaust.open @19:41:40.33 Intake.open @19:41:41.20 Pressure.in :default @19:41:41.26 Pressure.hold @19:41:41.48 SamplePump.in :sample @19:41:41.54 SamplePump.seek 75ml,5:31 @19:41:44.25 Cartridge 47 filtering 1/75ml at 0.32ml/s with 10.7psi vacuum @19:42:44.24 Cartridge 47 filtering 21/75ml at 0.32ml/s with 11.7psi vacuum @19:43:44.24 Cartridge 47 filtering 41/75ml at 0.33ml/s with 15.7psi vacuum @19:44:44.24 Cartridge 47 filtering 61/75ml at 0.30ml/s with 11.1psi vacuum @19:45:29.41 Pressure.stop @19:45:29.53 Intake.close @19:45:29.57 Exhaust.close @19:45:29.75 Toroid.in :hold @19:45:29.81 Toroid.seek :bypass @19:45:31.43 Toroid.in :default @19:45:31.49 Toroid.seek :clear @19:45:33.11 Sampled 75.0ml @19:45:34.52 -> Cmd.startProcessing @19:45:36.04 SPR.command \"read_sequences\" @19:45:36.12 SPR.command \"stats_on\" @19:45:36.16 SPR.command \"start 0 1\" @19:45:36.97 SPR.start /var/log/esp/spr21aug0619h.spr.bz2 @19:45:38.97 Handoff.select :waste @19:45:45.65 Syringe tare is 19% of full scale @19:45:45.67 PinPort.in :locking @19:45:45.74 PinPort.seek :locked @19:45:46.22 Archive.in :fast @19:45:46.28 Lysis2.in :fast @19:45:46.32 Lysis1.seek 1350uL @19:45:46.43 Archive.seek :full @19:45:46.48 Lysis2.seek :full @19:45:48.64 Plunge.seek :reagent @19:45:49.54 Evacuating puck with filter bubble point of 24psi @19:45:49.56 PinPort.seek :locked @19:45:49.66 Handoff.select :waste @19:45:56.25 Air syringe pumping up to 1 stroke to reach 8psi @19:45:56.26 Air stroke #1 of 1 @19:45:56.35 Air.in :pressurize @19:45:56.41 Air.seek :pressurized @19:46:07.67 -0.1psi after pressurize @19:46:07.69 Air.in :compress @19:46:07.76 Air.seek :evac @19:46:11.85 -0.1psi after compress @19:46:24.42 -0.1psi after compress (stable) @19:46:24.43 Air.in :default @19:46:24.50 Air.hold @19:46:24.67 at -0.1psi Opened to waste @19:46:24.68 Handoff.select :blocked @19:46:24.81 Archive syringe pumping up to 8 strokes to reach 24psi @19:46:24.82 Archive stroke #1 of 8 @19:46:24.90 Archive.in :pressurize @19:46:24.99 Archive.seek :pressurized @19:46:37.72 -0.1psi after pressurize @19:46:37.74 Archive.in :compress @19:46:37.80 Archive.seek :empty @19:46:53.69 1.4psi after compress @19:47:06.26 1.5psi after compress (stable) @19:47:06.28 Archive.in :gulp @19:47:06.35 Archive.seek :topVent @19:47:13.54 1.7psi after gulp @19:47:26.11 1.8psi after gulp (stable) @19:47:26.12 Archive stroke #2 of 8 @19:47:26.21 Archive.in :pressurize @19:47:26.27 Archive.seek :pressurized @19:47:39.02 1.9psi after pressurize @19:47:39.03 Archive.in :compress @19:47:39.11 Archive.seek :empty @19:47:54.99 2.1psi after compress @19:48:12.06 4.3psi after compress (stable) @19:48:12.08 Archive.in :gulp @19:48:12.15 Archive.seek :topVent @19:48:19.39 6.2psi after gulp @19:48:38.96 8.8psi after gulp (stable) @19:48:38.97 Archive stroke #3 of 8 @19:48:39.06 Archive.in :pressurize @19:48:39.12 Archive.seek :pressurized @19:48:51.87 10.1psi after pressurize @19:48:51.88 Archive.in :compress @19:48:51.96 Archive.seek :empty @19:49:07.83 11.0psi after compress @19:49:30.77 Reached 24.1 of 24.0psi after pumping 5190uL (3.00 strokes) @19:49:30.78 Archive.in :default @19:49:30.85 Archive.hold @19:49:30.91 Handoff.select :waste @19:49:41.63 delay 1:00 @19:50:41.65 Handoff.select :blocked @19:51:04.33 Handoff.select :waste @19:51:04.45 2.0psi at evacEnd @19:51:04.46 Handoff.select :blocked @19:51:04.59 Archive.in :fast @19:51:04.65 Archive.seek :topVent @19:51:18.35 Stable @2.8psi after Handoff blocked @19:51:18.37 Archive syringe pumping up to 8 strokes to reach 18psi @19:51:18.39 Archive stroke #1 of 8 @19:51:18.47 Archive.in :pressurize @19:51:18.54 Archive.seek :pressurized @19:51:31.26 4.3psi after pressurize @19:51:31.27 Archive.in :compress @19:51:31.37 Archive.seek :empty @19:51:47.24 5.6psi after compress @19:52:24.42 Reached 17.9 of 17.8psi after pumping 1730uL (1.00 strokes) @19:52:24.43 Archive.in :default @19:52:24.50 Archive.hold @19:52:24.61 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position @19:52:24.62 Handoff.select :waste @19:52:31.80 delay 1:00 @19:53:31.83 Handoff.select :blocked @19:53:54.50 Handoff.select :waste @19:53:54.62 1.4psi at evacEnd @19:53:54.64 Air.seek :fullVent @19:53:55.72 SPR.command \"start 2 14\" @19:53:56.49 SPR stabilized @19:53:58.62 Ended log spr21aug0619h.spr.bz2 @19:53:58.63 SPR.command \"stats_off\" @19:53:59.69 Air.setRawPosition! -7961 @19:53:59.74 Slide::Error in PROCESSING -- Air Syringe (AirS) positionErr at 84uL (sensed 114uL) @19:53:59.76 Thread::ChildDied in LRAUV -- :PROCESSING @19:54:03.18 -> Cmd.stop @19:54:03.26 Handoff.select :close @19:54:03.39 Gate.power :heat,:OFF @19:54:03.41 Gate.power :cartridge,:OFF @19:54:03.49 Carousel.seek 47 @19:54:03.68 Plunge.seek :locked @19:54:04.57 PinPort.in :unlocking @19:54:04.63 PinPort.seek :clear @19:54:05.17 Archive.in :unlocking @19:54:05.30 Archive.seek :clear @19:54:05.34 Air.in :unlocking @19:54:05.49 Air.seek :clear @19:54:05.56 Lysis1.in :unlocking @19:54:05.68 Lysis1.seek :clear @19:54:05.71 Lysis2.in :unlocking @19:54:05.82 Lysis2.seek :clear @19:54:09.51 Gate.power :motor,:OFF @19:54:09.53 Gate.power :core,:OFF @19:54:09.55 Gate.power :analytic1,:OFF @19:54:09.57 Gate.power :analytic2,:OFF @19:54:09.59 Gate.power :analytic3,:OFF @19:54:09.61 Gate.power :main,:OFF @19:54:09.63 Safely stopped and ready to power off ##-- file closed 2021-08-07T06:59:13.862Z ##-- file opened 2021-08-07T06:59:13.864Z @02:59:13.84 -> showStatus @02:59:15.85 -> Cmd.loadCartridge -4 @02:59:15.92 Gate.power :main,:ON,limit to 5000mA @02:59:15.96 Gate.power :analytic1,:ON,limit to 5000mA @02:59:15.98 Gate.power :analytic2,:ON,limit to 5000mA @02:59:16.01 Gate.power :analytic3,:ON,limit to 5000mA @02:59:16.15 Powering core bus @02:59:18.43 SPR Module 1, FW Rev. B, FPGA Rev. C @02:59:18.91 Archive.configure :default @02:59:19.03 Air.configure :default @02:59:19.13 Lysis1.configure :default @02:59:19.24 Lysis2.configure :default @02:59:19.36 Plunge.configure :default @02:59:19.48 SamplePump.configure :default @02:59:19.63 ExtraPump.configure :default @02:59:19.75 Toroid.configure :default @02:59:19.87 Carousel.configure :default @02:59:19.99 Pressure.configure :default @02:59:20.11 PinPort.configure :default @02:59:20.17 All elf controllers running firmware version 4.741 @02:59:20.20 Gate.power :motor,:ON,limit to 5000mA @02:59:20.49 Archive.in :default @02:59:20.63 Air.in :default @02:59:21.07 Lysis1.in :default @02:59:21.14 Lysis2.in :default @02:59:21.27 Archive.home.jog 0 @02:59:21.33 Plunge.in :default @02:59:21.40 Toroid.in :default @02:59:21.45 Air.home.jog 0 @02:59:21.49 Lysis1.home.jog 0 @02:59:21.53 Lysis2.home.jog 0 @02:59:21.56 PinPort.in :default @02:59:21.74 Plunge.home.jog 0 @02:59:21.78 Toroid.home.jog 0 @02:59:21.91 PinPort.home.jog 0 @02:59:22.45 Toroid.in :homing @02:59:22.50 Plunge.in :homing @02:59:22.53 Air.in :homing @02:59:22.57 PinPort.in :homing @02:59:22.60 Lysis1.in :homing @02:59:22.66 Lysis2.in :homing @02:59:22.69 Archive.in :homing @02:59:22.91 Toroid.jog 633 @02:59:22.94 Plunge.jog 1565 @02:59:22.97 PinPort.jog -109 @02:59:23.00 Lysis1.jog 1411 @02:59:23.02 Air.jog 181 @02:59:23.05 Lysis2.jog 1412 @02:59:23.09 Archive.jog 1375 @02:59:23.37 PinPort.setRawPosition! 0 @02:59:23.41 PinPort.seek :home @02:59:23.50 PinPort.in :default @02:59:23.63 Air.setRawPosition! 50 @02:59:23.68 Air.seek :home @02:59:23.96 Air.in :default @02:59:24.33 Plunge.setRawPosition! 50 @02:59:24.40 Plunge.seek :home @02:59:24.49 Lysis2.setRawPosition! 50 @02:59:24.51 Lysis1.setRawPosition! 50 @02:59:24.53 Archive.setRawPosition! 50 @02:59:24.62 Lysis2.seek :home @02:59:24.65 Lysis1.seek :home @02:59:24.75 Archive.seek :home @02:59:24.88 Plunge.in :default @02:59:25.00 Lysis2.in :default @02:59:25.11 Lysis1.in :default @02:59:25.14 Archive.in :default @02:59:25.25 Toroid.setRawPosition! 0 @02:59:25.31 Toroid.seek :home @02:59:25.40 Toroid.in :default @02:59:25.58 Toroid.seek :clear @02:59:27.19 Carousel.in :default @02:59:27.26 Carousel.home.jog 0 @02:59:27.74 Carousel.in :default @02:59:27.80 Carousel.seek 47 @02:59:27.97 Selecting Cartridge 46 @02:59:28.21 Carousel.seek 46 @02:59:28.70 Lysis1.in :fast @02:59:28.76 Lysis1.seek :locked @02:59:31.35 Plunge.seek :locked @02:59:31.47 Air.in :fast @02:59:31.53 Air.seek :locked @02:59:34.13 Powering cartridge bus @02:59:36.75 Gate.setIR 1750,5000,0,6 @02:59:36.88 Gate.setIR 0,5000,0,6 @02:59:36.93 Gate.power :cartridge,:OFF @02:59:37.47 Mounted archive Cartridge @02:59:55.48 -> Cmd.startFiltering @02:59:55.94 Priming sample loop w/100ml, bypass w/10ml @02:59:55.95 SamplePump.setPosition! 0ml @02:59:56.00 Bypass.open @02:59:57.48 Intake.open @02:59:58.27 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @03:00:00.83 Vacuum tare is 50% of full scale @03:00:00.96 Pressure.in :default @03:00:01.02 Pressure.hold @03:00:01.19 SamplePump.in :prime @03:00:01.25 SamplePump.seek 10ml,59 seconds @03:00:10.51 Pressure.stop @03:00:10.57 SamplePump.setPosition! 0ml @03:00:10.61 Exhaust.open @03:00:12.09 Bypass.close @03:00:13.62 Pressure.in :default @03:00:13.68 Pressure.hold @03:00:13.90 SamplePump.in :prime @03:00:13.97 SamplePump.seek 100ml,5:16 @03:02:18.01 Pressure.stop @03:02:18.07 Bypass.open @03:02:18.79 Intake.close @03:02:19.62 Toroid.in :hold @03:02:19.68 Toroid.seek :crack @03:02:21.19 Toroid.seek :cartridge @03:02:22.27 Toroid.in :default @03:02:22.33 Toroid.seek :clear @03:02:24.28 Pressurizing cartridge to 26psi above 14.3psia @03:02:24.29 SamplePump.setPosition! 0ml @03:02:24.34 Intake.open @03:02:25.06 Bypass.close @03:02:25.77 Exhaust.close @03:02:26.58 Pressure.in :default @03:02:26.64 Pressure.hold @03:02:26.81 SamplePump.in :charge @03:02:26.88 SamplePump.seek 2.3ml,42 seconds @03:02:33.32 Pressure.stop @03:02:33.37 Intake.close @03:02:34.19 Pressurized cartridge with 0.183ml to 28.4psi @03:02:34.20 Isolated @36.3psia (22.0psi) @03:02:34.22 Verifying cartridge holds at least 0.75..0.85 of 36.3psia @03:02:34.25 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @03:02:38.82 Cartridge pressure fell 1% to 35.9psia @03:02:38.90 Sampling 1000.0ml @03:02:38.91 SamplePump.setPosition! 0ml @03:02:38.96 Exhaust.open @03:02:39.64 Intake.open @03:02:39.97 Pressure.in :default @03:02:40.03 Pressure.hold @03:02:40.25 SamplePump.in :sample @03:02:40.32 SamplePump.seek 1000ml,1:23:53 @03:02:43.00 Cartridge 46 filtering 1/1000ml at 0.42ml/s with 22.7psi vacuum @03:03:43.00 Cartridge 46 filtering 24/1000ml at 0.42ml/s with 22.1psi vacuum @03:04:43.01 Cartridge 46 filtering 46/1000ml at 0.32ml/s with 24.1psi vacuum @03:05:43.00 Cartridge 46 filtering 68/1000ml at 0.35ml/s with 21.3psi vacuum @03:06:43.01 Cartridge 46 filtering 89/1000ml at 0.39ml/s with 22.8psi vacuum @03:07:43.00 Cartridge 46 filtering 109/1000ml at 0.32ml/s with 21.2psi vacuum @03:08:43.01 Cartridge 46 filtering 128/1000ml at 0.28ml/s with 18.2psi vacuum @03:09:43.00 Cartridge 46 filtering 147/1000ml at 0.31ml/s with 30.2psi vacuum @03:10:43.01 Cartridge 46 filtering 164/1000ml at 0.32ml/s with 21.8psi vacuum @03:11:43.00 Cartridge 46 filtering 181/1000ml at 0.28ml/s with 24.8psi vacuum @03:12:43.01 Cartridge 46 filtering 197/1000ml at 0.27ml/s with 22.8psi vacuum @03:13:43.00 Cartridge 46 filtering 212/1000ml at 0.25ml/s with 23.8psi vacuum @03:14:43.01 Cartridge 46 filtering 226/1000ml at 0.24ml/s with 24.7psi vacuum @03:15:43.00 Cartridge 46 filtering 240/1000ml at 0.23ml/s with 24.6psi vacuum @03:16:43.01 Cartridge 46 filtering 253/1000ml at 0.23ml/s with 27.2psi vacuum @03:17:43.00 Cartridge 46 filtering 266/1000ml at 0.20ml/s with 27.6psi vacuum @03:18:43.01 Cartridge 46 filtering 278/1000ml at 0.20ml/s with 25.6psi vacuum @03:19:35.26 Flow averaged 0.28ml/s over 16:56 @03:19:35.29 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @03:19:35.86 Relieved excess vacuum in .6 seconds @03:19:35.89 SamplePump.seek 1000ml,59:48 @03:19:43.00 Cartridge 46 filtering 290/1000ml at 0.20ml/s with 27.2psi vacuum @03:20:08.78 Flow averaged 0.20ml/s over 33 seconds @03:20:08.81 Waiting up to 15 seconds for vacuum to reduce from 28.8 to 18.0psi @03:20:09.38 Relieved excess vacuum in .6 seconds @03:20:09.41 SamplePump.seek 1000ml,59:15 @03:20:20.01 Flow averaged 0.20ml/s over 11 seconds @03:20:20.03 Intake.close @03:20:20.09 Exhaust.close @03:20:20.25 Toroid.in :hold @03:20:20.31 Toroid.seek :bypass @03:20:21.94 Toroid.in :default @03:20:22.00 Toroid.seek :clear @03:20:23.62 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @03:20:23.63 Pressure.stop @03:20:23.81 Sampled 297.6ml @03:20:26.09 -> Cmd.startProcessing @03:20:26.46 Plunge.seek :reagent @03:20:27.30 Sample loop closed. Ready to Deliver Reagents @03:20:27.31 Archive.seek :sealed @03:20:31.57 Archive.seek :evac @03:20:45.49 delay 1:00 @03:21:45.51 Archive.seek :clear @03:22:03.22 RNAlater soak for 10:00 @03:32:03.24 Archive.seek :tVent @03:32:05.82 delay 1 second @03:32:06.84 Archive.seek :evac @03:32:22.44 delay 10 seconds @03:32:32.47 Archive.seek :tVent @03:32:48.08 delay 1 second @03:32:49.10 Archive.seek :evac @03:33:04.70 delay 10 seconds @03:33:14.72 Sample preserved and evacuated @03:33:15.73 -> Cmd.stop @03:33:15.87 Carousel.seek 46 @03:33:16.05 Plunge.seek :locked @03:33:16.99 Archive.in :unlocking @03:33:17.12 Archive.seek :clear @03:33:17.15 Air.in :unlocking @03:33:17.30 Air.seek :clear @03:33:17.38 Lysis1.in :unlocking @03:33:17.49 Lysis1.seek :clear @03:33:21.36 Gate.power :motor,:OFF @03:33:21.38 Gate.power :core,:OFF @03:33:21.40 Gate.power :analytic1,:OFF @03:33:21.42 Gate.power :analytic2,:OFF @03:33:21.45 Gate.power :analytic3,:OFF @03:33:21.47 Gate.power :main,:OFF @03:33:21.49 Safely stopped and ready to power off ##-- file closed 2021-08-07T07:45:01.134Z ##-- file opened 2021-08-07T07:45:01.136Z @03:45:01.05 -> showStatus @03:45:03.07 -> Cmd.loadCartridge -18 @03:45:03.13 Gate.power :main,:ON,limit to 5000mA @03:45:03.15 Gate.power :analytic1,:ON,limit to 5000mA @03:45:03.17 Gate.power :analytic2,:ON,limit to 5000mA @03:45:03.19 Gate.power :analytic3,:ON,limit to 5000mA @03:45:03.33 Powering core bus @03:45:05.61 SPR Module 1, FW Rev. B, FPGA Rev. C @03:45:06.07 Archive.configure :default @03:45:06.20 Air.configure :default @03:45:06.31 Lysis1.configure :default @03:45:06.41 Lysis2.configure :default @03:45:06.53 Plunge.configure :default @03:45:06.66 SamplePump.configure :default @03:45:06.80 ExtraPump.configure :default @03:45:06.91 Toroid.configure :default @03:45:07.04 Carousel.configure :default @03:45:07.17 Pressure.configure :default @03:45:07.29 PinPort.configure :default @03:45:07.34 All elf controllers running firmware version 4.741 @03:45:07.37 Gate.power :motor,:ON,limit to 5000mA @03:45:07.61 Archive.in :default @03:45:07.67 Air.in :default @03:45:08.23 Archive.home.jog 0 @03:45:08.26 Air.home.jog 0 @03:45:08.42 Toroid.in :default @03:45:08.48 Plunge.in :default @03:45:08.52 Lysis1.in :default @03:45:08.55 PinPort.in :default @03:45:08.60 Lysis2.in :default @03:45:08.73 Toroid.home.jog 0 @03:45:08.78 Plunge.home.jog 0 @03:45:08.81 PinPort.home.jog 0 @03:45:08.85 Lysis1.home.jog 0 @03:45:08.89 Lysis2.home.jog 0 @03:45:09.46 Toroid.in :homing @03:45:09.48 Plunge.in :homing @03:45:09.51 Archive.in :homing @03:45:09.55 PinPort.in :homing @03:45:09.60 Lysis1.in :homing @03:45:09.64 Air.in :homing @03:45:09.82 Toroid.jog 632 @03:45:09.86 Plunge.jog 1573 @03:45:09.90 PinPort.jog -109 @03:45:09.93 Lysis1.jog 1415 @03:45:09.96 Archive.jog 1401 @03:45:10.00 Air.jog 1412 @03:45:10.24 Lysis2.in :homing @03:45:10.32 Lysis2.jog 1413 @03:45:10.34 PinPort.setRawPosition! 0 @03:45:10.41 PinPort.seek :home @03:45:10.54 PinPort.in :default @03:45:11.26 Lysis1.setRawPosition! 50 @03:45:11.32 Lysis1.seek :home @03:45:11.41 Plunge.setRawPosition! 50 @03:45:11.44 Air.setRawPosition! 50 @03:45:11.46 Archive.setRawPosition! 50 @03:45:11.57 Plunge.seek :home @03:45:11.60 Air.seek :home @03:45:11.70 Archive.seek :home @03:45:11.84 Lysis2.setRawPosition! 50 @03:45:11.86 Lysis1.in :default @03:45:11.96 Lysis2.seek :home @03:45:12.10 Plunge.in :default @03:45:12.13 Air.in :default @03:45:12.16 Archive.in :default @03:45:12.32 Toroid.setRawPosition! 0 @03:45:12.35 Lysis2.in :default @03:45:12.42 Toroid.seek :home @03:45:12.54 Toroid.in :default @03:45:12.77 Toroid.seek :clear @03:45:14.39 Carousel.in :default @03:45:14.45 Carousel.home.jog 0 @03:45:14.95 Carousel.in :default @03:45:15.01 Carousel.seek 46 @03:45:15.16 Selecting Cartridge 45 @03:45:15.41 Carousel.seek 45 @03:45:15.89 Lysis1.in :fast @03:45:15.96 Lysis1.seek :locked @03:45:18.55 Plunge.seek :locked @03:45:18.67 Air.in :fast @03:45:18.73 Air.seek :locked @03:45:21.33 Powering cartridge bus @03:45:23.96 Gate.setIR 1750,5000,0,6 @03:45:24.09 Gate.setIR 0,5000,0,6 @03:45:24.15 PuckHeater.configure :default @03:45:24.20 Gate.power :heat,:ON,limit to 5000mA @03:45:24.87 Mounted lysis Cartridge board#Q05036 @03:45:24.90 Using default puck heat loss curve for HeatingAccelInCarousel @03:45:43.47 -> Cmd.startFiltering @03:45:43.93 Priming sample loop w/100ml, bypass w/10ml @03:45:43.94 SamplePump.setPosition! 0ml @03:45:43.99 Bypass.open @03:45:45.47 Intake.open @03:45:46.30 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @03:45:48.87 Vacuum tare is 51% of full scale @03:45:48.99 Pressure.in :default @03:45:49.05 Pressure.hold @03:45:49.22 SamplePump.in :prime @03:45:49.29 SamplePump.seek 10ml,59 seconds @03:45:58.55 Pressure.stop @03:45:58.60 SamplePump.setPosition! 0ml @03:45:58.65 Exhaust.open @03:46:00.10 Bypass.close @03:46:01.66 Pressure.in :default @03:46:01.72 Pressure.hold @03:46:01.94 SamplePump.in :prime @03:46:02.00 SamplePump.seek 100ml,5:16 @03:48:05.77 Pressure.stop @03:48:05.83 Bypass.open @03:48:06.55 Intake.close @03:48:07.41 Toroid.in :hold @03:48:07.47 Toroid.seek :crack @03:48:08.98 Toroid.seek :cartridge @03:48:10.06 Toroid.in :default @03:48:10.12 Toroid.seek :clear @03:48:12.08 Pressurizing cartridge to 14psi above 14.5psia @03:48:12.09 SamplePump.setPosition! 0ml @03:48:12.13 Intake.open @03:48:12.32 Bypass.close @03:48:13.05 Exhaust.close @03:48:13.85 Pressure.in :default @03:48:13.91 Pressure.hold @03:48:14.08 SamplePump.in :charge @03:48:14.15 SamplePump.seek 2.3ml,41 seconds @03:48:24.52 Pressure.stop @03:48:24.58 Intake.close @03:48:25.42 Pressurized cartridge with 0.479ml to 14.1psi @03:48:25.44 Isolated @25.8psia (11.4psi) @03:48:25.45 Verifying cartridge holds at least 0.75..0.85 of 25.8psia @03:48:25.49 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @03:48:30.06 Cartridge pressure fell 7% to 23.9psia @03:48:30.13 Sampling 75.0ml @03:48:30.15 SamplePump.setPosition! 0ml @03:48:30.20 Exhaust.open @03:48:30.89 Intake.open @03:48:31.77 Pressure.in :default @03:48:31.83 Pressure.hold @03:48:32.46 SamplePump.in :sample @03:48:32.53 SamplePump.seek 75ml,5:31 @03:48:34.81 Cartridge 45 filtering 1/75ml at 0.36ml/s with 12.2psi vacuum @03:49:34.81 Cartridge 45 filtering 21/75ml at 0.33ml/s with 10.6psi vacuum @03:50:34.81 Cartridge 45 filtering 42/75ml at 0.31ml/s with 11.2psi vacuum @03:51:34.81 Cartridge 45 filtering 62/75ml at 0.31ml/s with 12.7psi vacuum @03:52:15.48 Pressure.stop @03:52:15.60 Intake.close @03:52:15.64 Exhaust.close @03:52:15.81 Toroid.in :hold @03:52:15.87 Toroid.seek :bypass @03:52:17.48 Toroid.in :default @03:52:17.54 Toroid.seek :clear @03:52:19.16 Sampled 75.0ml @03:52:20.62 -> Cmd.startProcessing @03:52:22.15 SPR.command \"read_sequences\" @03:52:22.46 SPR.command \"stats_on\" @03:52:22.50 SPR.command \"start 0 1\" @03:52:23.31 SPR.start /var/log/esp/spr21aug0703h.spr.bz2 @03:52:25.82 Handoff.select :waste @03:52:32.52 Syringe tare is 19% of full scale @03:52:32.55 PinPort.in :locking @03:52:32.62 PinPort.seek :locked @03:52:33.09 Archive.in :fast @03:52:33.15 Lysis2.in :fast @03:52:33.19 Lysis1.seek 1350uL @03:52:33.30 Archive.seek :full @03:52:33.40 Lysis2.seek :full @03:52:35.47 Plunge.seek :reagent @03:52:36.38 Evacuating puck with filter bubble point of 24psi @03:52:36.40 PinPort.seek :locked @03:52:36.48 Handoff.select :waste @03:52:43.06 Air syringe pumping up to 1 stroke to reach 8psi @03:52:43.07 Air stroke #1 of 1 @03:52:43.16 Air.in :pressurize @03:52:43.22 Air.seek :pressurized @03:52:54.47 1.1psi after pressurize @03:52:54.48 Air.in :compress @03:52:54.56 Air.seek :evac @03:52:58.65 1.2psi after compress @03:53:11.24 0.8psi after compress (stable) @03:53:11.26 Air.in :default @03:53:11.33 Air.hold @03:53:11.50 at 0.5psi Opened to waste @03:53:11.51 Handoff.select :blocked @03:53:11.65 Archive syringe pumping up to 8 strokes to reach 24psi @03:53:11.66 Archive stroke #1 of 8 @03:53:11.74 Archive.in :pressurize @03:53:11.80 Archive.seek :pressurized @03:53:24.53 3.2psi after pressurize @03:53:24.54 Archive.in :compress @03:53:24.61 Archive.seek :empty @03:53:40.49 9.5psi after compress @03:54:02.09 16.6psi after compress (stable) @03:54:02.10 Archive.in :gulp @03:54:02.17 Archive.seek :topVent @03:54:09.37 16.5psi after gulp @03:54:22.37 16.5psi after gulp (stable) @03:54:22.38 Archive stroke #2 of 8 @03:54:22.47 Archive.in :pressurize @03:54:22.53 Archive.seek :pressurized @03:54:35.27 16.4psi after pressurize @03:54:35.28 Archive.in :compress @03:54:35.35 Archive.seek :empty @03:54:47.94 Reached 24.1 of 24.0psi after pumping 3390uL (1.96 strokes) @03:54:47.95 Archive.in :default @03:54:48.02 Archive.hold @03:54:48.08 Handoff.select :waste @03:55:00.77 delay 1:00 @03:56:00.79 Handoff.select :blocked @03:56:23.47 Handoff.select :waste @03:56:23.59 0.3psi at evacEnd @03:56:23.60 Handoff.select :blocked @03:56:23.73 Archive.in :fast @03:56:23.81 Archive.seek :topVent @03:56:37.26 Stable @0.3psi after Handoff blocked @03:56:37.28 Archive syringe pumping up to 8 strokes to reach 15psi @03:56:37.30 Archive stroke #1 of 8 @03:56:37.41 Archive.in :pressurize @03:56:37.49 Archive.seek :pressurized @03:56:50.23 0.3psi after pressurize @03:56:50.25 Archive.in :compress @03:56:50.31 Archive.seek :empty @03:57:06.20 13.0psi after compress @03:57:18.77 13.9psi after compress (stable) @03:57:18.79 Archive.in :gulp @03:57:18.86 Archive.seek :topVent @03:57:26.06 13.9psi after gulp @03:57:38.63 13.9psi after gulp (stable) @03:57:38.64 Archive stroke #2 of 8 @03:57:38.73 Archive.in :pressurize @03:57:38.79 Archive.seek :pressurized @03:57:51.52 13.8psi after pressurize @03:57:51.53 Archive.in :compress @03:57:51.61 Archive.seek :empty @03:57:57.59 Reached 15.4 of 15.3psi after pumping 3248uL (1.88 strokes) @03:57:57.60 Archive.in :default @03:57:57.67 Archive.hold @03:57:57.77 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position @03:57:57.81 Handoff.select :waste @03:58:05.00 delay 1:00 @03:59:05.02 Handoff.select :blocked @03:59:27.70 Handoff.select :waste @03:59:27.82 0.4psi at evacEnd @03:59:27.84 Air.seek :fullVent @03:59:29.28 Archive.in :fast @03:59:29.34 Archive.seek :topVent @03:59:35.74 Seawater evacuated, Presure above/below filter released. Lysis begins @03:59:35.83 Lysis1.in :slow @03:59:35.89 Lysis1.seek 1050uL @04:00:05.89 delay 5 seconds @04:00:10.91 Handoff.select :blocked @04:00:11.07 Lysis1.seek 450uL @04:01:10.97 delay 3 seconds @04:01:14.04 Air.in to mix into 1.37806 parts Lysis1 @04:01:14.63 Air.seek :evac @04:01:14.67 Lysis1.seek 300uL @04:01:29.77 Lysis1.seek :empty @04:01:59.85 Lysis buffer delivered @20.7psi @04:01:59.86 Polling Can every 2:00 @04:02:00.41 PuckHeater.configure :default #ambient 24.4C @04:02:00.46 PuckHeater.seek 79.6C @04:02:48.98 PuckHeater.hold 2:00,79.6C @04:04:48.89 Polling Can every 2:00 @04:04:49.43 PuckHeater.configure :default #ambient 24.5C @04:04:49.48 PuckHeater.hold 30 seconds,78.6C @04:05:19.63 Polling Can every 2:00 @04:05:20.17 PuckHeater.hold 30 seconds,78.1C @04:05:50.32 Polling Can every 2:00 @04:05:50.86 PuckHeater.configure :default #ambient 24.5C @04:05:50.91 PuckHeater.hold 10:00,77.6C @04:15:50.05 Lysis complete. Syringe pressure @20.3psi @04:15:50.06 Handoff.select :blocked @04:15:50.10 Archive syringe pumping up to 5 strokes to reach 40psi @04:15:50.12 Archive stroke #1 of 5 @04:15:50.20 Archive.in :pressurize @04:15:50.27 Archive.seek :pressurized @04:16:02.99 20.2psi after pressurize @04:16:03.00 Archive.in :compress @04:16:03.07 Archive.seek :empty @04:16:11.18 Reached 40.3 of 40.0psi after pumping 1564uL (0.90 strokes) @04:16:11.21 Archive.in :default @04:16:11.29 Archive.hold @04:16:11.36 delay 2 seconds @04:16:13.38 Archive.in :fast @04:16:13.44 Archive.seek :full @04:16:20.07 Lysis1.seek 138uL @04:16:33.94 delay 1 second @04:16:34.97 Archive.seek :full @04:16:34.99 Lysis1.seek :empty @04:16:48.96 Initial air pressure @46.2psi @04:16:49.05 Lysis1.in :extract @04:16:49.53 Lysis1.seek 600uL,nil @04:16:55.19 Archive syringe pumping up to 6 strokes to reach 25psi @04:16:55.20 Archive stroke #1 of 6 @04:16:55.28 Archive.in :pressurize @04:16:55.34 Archive.seek :pressurized @04:17:08.07 14.0psi after pressurize @04:17:08.09 Archive.in :compress @04:17:08.16 Archive.seek :empty @04:17:15.33 Archive.in :default @04:17:15.38 Archive.hold @04:17:15.63 Initial 600uL lysate @16.4psi @04:17:15.64 Handoff.select :blocked @04:17:15.74 Initial air pressure @16.7psi @04:17:15.82 Lysis1.seek 825uL,nil @04:17:19.24 Archive syringe pumping up to 6 strokes to reach 17psi @04:17:19.25 Archive stroke #1 of 6 @04:17:19.36 Archive.in :compress @04:17:19.43 Archive.seek :empty @04:17:20.86 Archive.in :default @04:17:20.93 Archive.hold @04:17:21.22 Recovered 825uL lysate @15.2psi @04:17:21.25 Air.in :halfSpeed @04:17:21.32 Air.seek :fullVent @04:17:23.57 Puck Top Vented, bottom @10.6psi @04:17:23.58 Handoff.select :waste @04:17:23.70 delay 5 seconds @04:17:28.77 Handoff open to waste, bottom @0.1psi @04:17:28.80 Air.in :default @04:17:28.87 Air.seek :evac @04:17:30.31 Lysis2.in :DAmix @04:17:30.41 Lysis1.in :DAmix @04:17:30.94 Lysis2.seek 1480uL @04:17:31.00 Lysis1.seek 575uL @04:18:06.04 delay 5 seconds @04:18:11.06 Handoff.select SPR @04:18:11.18 SPR.command \"start 2 14\" @04:18:11.79 SPR stabilized @04:18:11.92 delay 10 seconds @04:18:21.94 SPR.command \"start 2 0\" @04:18:25.14 Waiting up to 11:00 for SPR to enter state 1 @04:20:22.81 SPR.command \"hold_on\" @04:20:24.20 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @04:20:24.64 Lysis2.seek 1180uL @04:20:24.71 Lysis1.seek 275uL @04:21:06.70 delay 15 seconds @04:21:21.72 SPR.command \"hold_off\" @04:21:24.86 Waiting up to 38:00 for SPR to enter state 7 @04:45:22.01 SPR analysis completed @04:45:26.30 Ended log spr21aug0703h.spr.bz2 @04:45:26.31 SPR.command \"stats_off\" @04:45:27.91 -> Cmd.stop @04:45:27.92 Archive.stop @04:45:28.04 Handoff.select :close @04:45:28.17 Gate.power :heat,:OFF @04:45:28.19 Gate.power :cartridge,:OFF @04:45:28.27 Carousel.seek 45 @04:45:28.45 Plunge.seek :locked @04:45:29.33 PinPort.in :unlocking @04:45:29.39 PinPort.seek :clear @04:45:29.94 Archive.in :unlocking @04:45:30.06 Air.in :unlocking @04:45:30.10 Archive.seek :clear @04:45:30.25 Air.seek :clear @04:45:30.32 Lysis1.in :unlocking @04:45:30.44 Lysis1.seek :clear @04:45:30.48 Lysis2.in :unlocking @04:45:30.58 Lysis2.seek :clear @04:45:34.19 Gate.power :motor,:OFF @04:45:34.21 Gate.power :core,:OFF @04:45:34.23 Gate.power :analytic1,:OFF @04:45:34.25 Gate.power :analytic2,:OFF @04:45:34.27 Gate.power :analytic3,:OFF @04:45:34.29 Gate.power :main,:OFF @04:45:34.31 Safely stopped and ready to power off ##-- file closed 2021-08-07T17:09:18.982Z