##-- file opened 2021-08-06T00:28:40.093Z
@20:28:41.29 -> showStatus
@20:28:42.90 -> Cmd.loadCartridge -4
@20:28:42.96 Gate.power :main,:ON,limit to 5000mA
@20:28:42.97 Gate.power :analytic1,:ON,limit to 5000mA
@20:28:43.01 Gate.power :analytic2,:ON,limit to 5000mA
@20:28:43.05 Gate.power :analytic3,:ON,limit to 5000mA
@20:28:43.19 Powering core bus
@20:28:45.47 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@20:28:45.93 <LRAUV> Archive.configure :default
@20:28:46.06 Air.configure :default
@20:28:46.16 Lysis1.configure :default
@20:28:46.27 Lysis2.configure :default
@20:28:46.39 Plunge.configure :default
@20:28:46.52 SamplePump.configure :default
@20:28:46.66 ExtraPump.configure :default
@20:28:46.78 Toroid.configure :default
@20:28:46.90 Carousel.configure :default
@20:28:47.02 Pressure.configure :default
@20:28:47.14 PinPort.configure :default
@20:28:47.20 All elf controllers running firmware version 4.741
@20:28:47.23 Gate.power :motor,:ON,limit to 5000mA
@20:28:47.53 <Archive_home> Archive.in :default
@20:28:47.57 <Air_home> Air.in :default
@20:28:47.78 Air.home.jog 0
@20:28:47.81 <Archive_home> Archive.home.jog 0
@20:28:48.05 <PinPort_home> PinPort.in :default
@20:28:48.08 <Toroid_home> Toroid.in :default
@20:28:48.12 <Plunge_home> Plunge.in :default
@20:28:48.15 <Lysis2_home> Lysis2.in :default
@20:28:48.27 <Lysis1_home> Lysis1.in :default
@20:28:48.38 <PinPort_home> PinPort.home.jog 0
@20:28:48.43 <Toroid_home> Toroid.home.jog 0
@20:28:48.46 <Plunge_home> Plunge.home.jog 0
@20:28:48.83 <Lysis2_home> Lysis2.home.jog 0
@20:28:48.90 <Lysis1_home> Lysis1.home.jog 0
@20:28:49.25 <Archive_home> Archive.in :homing
@20:28:49.35 <Air_home> Air.in :homing
@20:28:49.44 <Archive_home> Archive.jog 1395
@20:28:49.48 <Air_home> Air.jog 1412
@20:28:49.67 <PinPort_home> PinPort.in :homing
@20:28:49.70 <Toroid_home> Toroid.in :homing
@20:28:49.74 <Lysis1_home> Lysis1.in :homing
@20:28:49.77 <Lysis2_home> Lysis2.in :homing
@20:28:49.82 <Plunge_home> Plunge.in :homing
@20:28:49.93 <PinPort_home> PinPort.jog -108
@20:28:50.02 <Toroid_home> Toroid.jog 622
@20:28:50.05 <Lysis1_home> Lysis1.jog 1412
@20:28:50.08 <Lysis2_home> Lysis2.jog 1410
@20:28:50.13 <Plunge_home> Plunge.jog 109
@20:28:50.33 <PinPort_home> PinPort.setRawPosition! 0
@20:28:50.38 PinPort.seek :home
@20:28:50.68 <Plunge_home> Plunge.setRawPosition! 50
@20:28:50.71 <PinPort_home> PinPort.in :default
@20:28:50.75 <Archive_home> Archive.setRawPosition! 50
@20:28:50.81 <Plunge_home> Plunge.seek :home
@20:28:50.93 <Archive_home> Archive.seek :home
@20:28:51.02 <Air_home> Air.setRawPosition! 50
@20:28:51.06 Air.seek :home
@20:28:51.15 <Plunge_home> Plunge.in :default
@20:28:51.25 <Archive_home> Archive.in :default
@20:28:51.37 <Lysis2_home> Lysis2.setRawPosition! 50
@20:28:51.40 <Lysis1_home> Lysis1.setRawPosition! 50
@20:28:51.42 <Air_home> Air.in :default
@20:28:51.52 <Lysis2_home> Lysis2.seek :home
@20:28:51.60 <Lysis1_home> Lysis1.seek :home
@20:28:51.85 <Lysis2_home> Lysis2.in :default
@20:28:51.94 <Lysis1_home> Lysis1.in :default
@20:28:52.26 <Toroid_home> Toroid.setRawPosition! 0
@20:28:52.30 Toroid.seek :home
@20:28:52.40 Toroid.in :default
@20:28:52.53 <LRAUV> Toroid.seek :clear
@20:28:54.14 Carousel.in :default
@20:28:54.20 Carousel.home.jog 0
@20:28:54.69 Carousel.in :default
@20:28:54.75 Carousel.seek 51
@20:28:55.04 Selecting Cartridge 52
@20:28:55.28 Carousel.seek 52
@20:28:55.76 Lysis1.in :fast
@20:28:55.82 Lysis1.seek :locked
@20:28:58.41 <Plunge> Plunge.seek :locked
@20:28:58.53 <Air> Air.in :fast
@20:28:58.60 Air.seek :locked
@20:29:01.19 <LRAUV> Powering cartridge bus
@20:29:03.81 Gate.setIR 1750,5000,0,6
@20:29:03.94 Gate.setIR 0,5000,0,6
@20:29:03.99 Gate.power :cartridge,:OFF
@20:29:04.53 Mounted archive Cartridge
@20:29:22.55 -> Cmd.startFiltering
@20:29:23.01 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@20:29:23.02 SamplePump.setPosition! 0ml
@20:29:23.07 Bypass.open
@20:29:24.57 Intake.open
@20:29:25.37 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@20:29:27.94 Vacuum tare is 50% of full scale
@20:29:28.07 Pressure.in :default
@20:29:28.13 Pressure.hold
@20:29:28.30 SamplePump.in :prime
@20:29:28.36 SamplePump.seek 10ml,59 seconds
@20:29:37.63 Pressure.stop
@20:29:37.68 SamplePump.setPosition! 0ml
@20:29:37.73 Exhaust.open
@20:29:39.18 <closeBypass> Bypass.close
@20:29:40.73 <FILTERING> Pressure.in :default
@20:29:40.79 Pressure.hold
@20:29:41.01 SamplePump.in :prime
@20:29:41.08 SamplePump.seek 100ml,5:16
@20:31:39.39 Pressure.stop
@20:31:39.45 Bypass.open
@20:31:40.17 Intake.close
@20:31:40.99 Toroid.in :hold
@20:31:41.05 Toroid.seek :crack
@20:31:42.57 Toroid.seek :cartridge
@20:31:43.66 Toroid.in :default
@20:31:43.72 Toroid.seek :clear
@20:31:45.69 Pressurizing cartridge to 26psi above 15.9psia
@20:31:45.71 SamplePump.setPosition! 0ml
@20:31:45.75 Intake.open
@20:31:46.48 Bypass.close
@20:31:47.17 Exhaust.close
@20:31:47.95 Pressure.in :default
@20:31:48.01 Pressure.hold
@20:31:48.18 SamplePump.in :charge
@20:31:48.25 SamplePump.seek 2.3ml,42 seconds
@20:31:55.28 Pressure.stop
@20:31:55.33 Intake.close
@20:31:56.13 Pressurized cartridge with 0.218ml to 28.1psi
@20:31:56.14 Isolated @39.0psia (23.1psi)
@20:31:56.15 Verifying cartridge holds at least 0.75..0.85 of 39.0psia
@20:31:56.18 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@20:32:00.75 Cartridge pressure fell 1% to 38.7psia
@20:32:00.83 Sampling 1000.0ml
@20:32:00.84 SamplePump.setPosition! 0ml
@20:32:00.89 Exhaust.open
@20:32:01.57 Intake.open
@20:32:02.40 Pressure.in :default
@20:32:02.47 Pressure.hold
@20:32:02.59 Sea 1.6m deep (17.0psia)
@20:32:02.72 SamplePump.in :sample
@20:32:02.79 SamplePump.seek 1000ml,1:23:53
@20:32:05.44 <sampling> Cartridge 52 filtering 1/1000ml at 0.43ml/s with 21.2psi vacuum
@20:33:05.44 Cartridge 52 filtering 25/1000ml at 0.39ml/s with 22.4psi vacuum
@20:34:05.45 Cartridge 52 filtering 48/1000ml at 0.38ml/s with 22.6psi vacuum
@20:35:05.44 Cartridge 52 filtering 70/1000ml at 0.33ml/s with 24.6psi vacuum
@20:36:05.45 Cartridge 52 filtering 92/1000ml at 0.31ml/s with 23.9psi vacuum
@20:37:05.45 Cartridge 52 filtering 113/1000ml at 0.31ml/s with 28.1psi vacuum
@20:38:05.45 Cartridge 52 filtering 134/1000ml at 0.38ml/s with 21.7psi vacuum
@20:39:05.87 Cartridge 52 filtering 154/1000ml at 0.34ml/s with 27.4psi vacuum
@20:40:05.45 Cartridge 52 filtering 172/1000ml at 0.31ml/s with 21.6psi vacuum
@20:41:05.45 Cartridge 52 filtering 191/1000ml at 0.26ml/s with 28.2psi vacuum
@20:42:05.45 Cartridge 52 filtering 208/1000ml at 0.31ml/s with 18.3psi vacuum
@20:43:05.45 Cartridge 52 filtering 224/1000ml at 0.27ml/s with 21.5psi vacuum
@20:44:05.45 Cartridge 52 filtering 240/1000ml at 0.20ml/s with 26.3psi vacuum
@20:45:05.45 Cartridge 52 filtering 254/1000ml at 0.20ml/s with 27.5psi vacuum
@20:46:05.45 Cartridge 52 filtering 268/1000ml at 0.23ml/s with 23.9psi vacuum
@20:47:05.45 Cartridge 52 filtering 281/1000ml at 0.25ml/s with 24.6psi vacuum
@20:48:05.45 Cartridge 52 filtering 294/1000ml at 0.24ml/s with 24.7psi vacuum
@20:49:05.45 Cartridge 52 filtering 306/1000ml at 0.23ml/s with 23.5psi vacuum
@20:49:06.09 <FILTERING> Flow averaged 0.30ml/s over 17:04
@20:49:06.12 Waiting up to 16 seconds for vacuum to reduce from 29.1 to 18.0psi
@20:49:06.70 Relieved excess vacuum in .6 seconds
@20:49:06.72 SamplePump.seek 1000ml,58:20
@20:49:24.68 Flow averaged 0.20ml/s over 18 seconds
@20:49:24.71 Waiting up to 14 seconds for vacuum to reduce from 28.2 to 18.0psi
@20:49:25.28 Relieved excess vacuum in .6 seconds
@20:49:25.31 SamplePump.seek 1000ml,58:02
@20:49:35.94 Flow averaged 0.20ml/s over 11 seconds
@20:49:35.97 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi
@20:49:36.53 Relieved excess vacuum in .6 seconds
@20:49:36.56 SamplePump.seek 1000ml,57:51
@20:49:50.03 Flow averaged 0.20ml/s over 14 seconds
@20:49:50.06 Waiting up to 14 seconds for vacuum to reduce from 28.3 to 18.0psi
@20:49:50.63 Relieved excess vacuum in .6 seconds
@20:49:50.66 SamplePump.seek 1000ml,57:37
@20:50:02.71 Flow averaged 0.20ml/s over 13 seconds
@20:50:02.74 Waiting up to 15 seconds for vacuum to reduce from 28.8 to 18.0psi
@20:50:03.31 Relieved excess vacuum in .6 seconds
@20:50:03.34 SamplePump.seek 1000ml,57:25
@20:50:05.44 <sampling> Cartridge 52 filtering 318/1000ml at 0.20ml/s with 23.7psi vacuum
@20:50:09.50 <FILTERING> Flow estimate reduced to 0.16ml/s
@20:50:09.51 <closeIntake> Intake.close
@20:50:09.56 <closeExhaust> Exhaust.close
@20:50:09.75 <FILTERING> Toroid.in :hold
@20:50:09.82 Toroid.seek :bypass
@20:50:11.45 Toroid.in :default
@20:50:11.51 Toroid.seek :clear
@20:50:13.12 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@20:50:13.13 Pressure.stop
@20:50:13.32 Sampled  318.6ml
@20:50:15.37 <LRAUV> -> Cmd.startProcessing
@20:50:15.74 <PROCESSING> Plunge.seek :reagent
@20:50:16.57 Sample loop closed. Ready to Deliver Reagents
@20:50:16.58 Archive.seek :sealed
@20:50:20.85 Archive.seek :evac
@20:50:34.79 delay 1:00
@20:51:34.82 Archive.seek :clear
@20:51:52.53 RNAlater soak for 10:00
@21:01:52.56 Archive.seek :tVent
@21:01:55.13 delay 1 second
@21:01:56.15 Archive.seek :evac
@21:02:11.76 delay 10 seconds
@21:02:21.79 Archive.seek :tVent
@21:02:37.40 delay 1 second
@21:02:38.42 Archive.seek :evac
@21:02:54.03 delay 10 seconds
@21:03:04.06 Sample preserved and evacuated
@21:03:05.02 <LRAUV> -> Cmd.stop
@21:03:05.16 Carousel.seek 52
@21:03:05.33 Plunge.seek :locked
@21:03:06.28 <Archive> Archive.in :unlocking
@21:03:06.41 Archive.seek :clear
@21:03:06.45 <Air> Air.in :unlocking
@21:03:06.60 Air.seek :clear
@21:03:06.68 <Lysis1> Lysis1.in :unlocking
@21:03:06.79 Lysis1.seek :clear
@21:03:10.61 <LRAUV> Gate.power :motor,:OFF
@21:03:10.63 Gate.power :core,:OFF
@21:03:10.65 Gate.power :analytic1,:OFF
@21:03:10.67 Gate.power :analytic2,:OFF
@21:03:10.69 Gate.power :analytic3,:OFF
@21:03:10.71 Gate.power :main,:OFF
@21:03:10.74 Safely stopped and ready to power off
##-- file closed 2021-08-06T01:12:07.183Z

##-- file opened 2021-08-06T01:12:07.190Z
@21:12:07.32 -> showStatus
@21:12:09.33 -> Cmd.loadCartridge -18
@21:12:09.39 Gate.power :main,:ON,limit to 5000mA
@21:12:09.41 Gate.power :analytic1,:ON,limit to 5000mA
@21:12:09.43 Gate.power :analytic2,:ON,limit to 5000mA
@21:12:09.45 Gate.power :analytic3,:ON,limit to 5000mA
@21:12:09.59 Powering core bus
@21:12:11.87 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@21:12:12.34 <LRAUV> Archive.configure :default
@21:12:12.47 Air.configure :default
@21:12:12.57 Lysis1.configure :default
@21:12:12.67 Lysis2.configure :default
@21:12:12.79 Plunge.configure :default
@21:12:12.92 SamplePump.configure :default
@21:12:13.06 ExtraPump.configure :default
@21:12:13.17 Toroid.configure :default
@21:12:13.29 Carousel.configure :default
@21:12:13.42 Pressure.configure :default
@21:12:13.53 PinPort.configure :default
@21:12:13.59 All elf controllers running firmware version 4.741
@21:12:13.62 Gate.power :motor,:ON,limit to 5000mA
@21:12:13.80 <Archive_home> Archive.in :default
@21:12:14.09 Archive.home.jog 0
@21:12:14.25 <Toroid_home> Toroid.in :default
@21:12:14.33 <Plunge_home> Plunge.in :default
@21:12:14.36 <Lysis1_home> Lysis1.in :default
@21:12:14.41 <PinPort_home> PinPort.in :default
@21:12:14.48 <Lysis2_home> Lysis2.in :default
@21:12:14.51 <Air_home> Air.in :default
@21:12:14.70 <Toroid_home> Toroid.home.jog 0
@21:12:14.72 <Plunge_home> Plunge.home.jog 0
@21:12:15.12 <Lysis2_home> Lysis2.home.jog 0
@21:12:15.16 <Lysis1_home> Lysis1.home.jog 0
@21:12:15.20 <PinPort_home> PinPort.home.jog 0
@21:12:15.23 <Air_home> Air.home.jog 0
@21:12:15.69 <Archive_home> Archive.in :homing
@21:12:15.92 <Toroid_home> Toroid.in :homing
@21:12:15.96 <Plunge_home> Plunge.in :homing
@21:12:15.99 <Archive_home> Archive.jog 1401
@21:12:16.03 <PinPort_home> PinPort.in :homing
@21:12:16.06 <Lysis2_home> Lysis2.in :homing
@21:12:16.10 <Lysis1_home> Lysis1.in :homing
@21:12:16.14 <Air_home> Air.in :homing
@21:12:16.36 <Plunge_home> Plunge.jog 1569
@21:12:16.39 <Toroid_home> Toroid.jog 625
@21:12:16.42 <PinPort_home> PinPort.jog -108
@21:12:16.45 <Lysis2_home> Lysis2.jog 1412
@21:12:16.47 <Lysis1_home> Lysis1.jog 1414
@21:12:16.51 <Air_home> Air.jog 1410
@21:12:16.81 <PinPort_home> PinPort.setRawPosition! 0
@21:12:16.85 PinPort.seek :home
@21:12:16.94 PinPort.in :default
@21:12:17.38 <Archive_home> Archive.setRawPosition! 50
@21:12:17.44 Archive.seek :home
@21:12:17.71 Archive.in :default
@21:12:17.84 <Plunge_home> Plunge.setRawPosition! 50
@21:12:17.87 <Lysis2_home> Lysis2.setRawPosition! 50
@21:12:17.89 <Lysis1_home> Lysis1.setRawPosition! 50
@21:12:17.91 <Air_home> Air.setRawPosition! 50
@21:12:18.02 <Plunge_home> Plunge.seek :home
@21:12:18.09 <Lysis2_home> Lysis2.seek :home
@21:12:18.12 <Air_home> Air.seek :home
@21:12:18.16 <Lysis1_home> Lysis1.seek :home
@21:12:18.46 <Plunge_home> Plunge.in :default
@21:12:18.56 <Lysis2_home> Lysis2.in :default
@21:12:18.60 <Lysis1_home> Lysis1.in :default
@21:12:18.63 <Air_home> Air.in :default
@21:12:18.80 <Toroid_home> Toroid.setRawPosition! 0
@21:12:18.84 Toroid.seek :home
@21:12:18.94 Toroid.in :default
@21:12:19.11 <LRAUV> Toroid.seek :clear
@21:12:20.72 Carousel.in :default
@21:12:20.78 Carousel.home.jog 0
@21:12:21.27 Carousel.in :default
@21:12:21.33 Carousel.seek 52
@21:12:21.48 Selecting Cartridge 51
@21:12:21.72 Carousel.seek 51
@21:12:22.21 Lysis1.in :fast
@21:12:22.27 Lysis1.seek :locked
@21:12:24.86 <Plunge> Plunge.seek :locked
@21:12:24.98 <Air> Air.in :fast
@21:12:25.04 Air.seek :locked
@21:12:27.65 <LRAUV> Powering cartridge bus
@21:12:30.28 Gate.setIR 1750,5000,0,6
@21:12:30.41 Gate.setIR 0,5000,0,6
@21:12:30.47 PuckHeater.configure :default
@21:12:30.52 Gate.power :heat,:ON,limit to 5000mA
@21:12:31.19 Mounted lysis Cartridge board#Q05051
@21:12:31.22 Using default puck heat loss curve for HeatingAccelInCarousel
@21:12:49.75 -> Cmd.startFiltering
@21:12:50.21 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@21:12:50.23 SamplePump.setPosition! 0ml
@21:12:50.28 Bypass.open
@21:12:51.74 Intake.open
@21:12:52.51 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@21:12:55.08 Vacuum tare is 50% of full scale
@21:12:55.20 Pressure.in :default
@21:12:55.26 Pressure.hold
@21:12:55.43 SamplePump.in :prime
@21:12:55.50 SamplePump.seek 10ml,59 seconds
@21:13:04.76 Pressure.stop
@21:13:04.81 SamplePump.setPosition! 0ml
@21:13:04.86 Exhaust.open
@21:13:06.30 <closeBypass> Bypass.close
@21:13:07.85 <FILTERING> Pressure.in :default
@21:13:07.91 Pressure.hold
@21:13:08.12 SamplePump.in :prime
@21:13:08.19 SamplePump.seek 100ml,5:16
@21:15:10.33 Pressure.stop
@21:15:10.39 Bypass.open
@21:15:11.14 Intake.close
@21:15:11.94 Toroid.in :hold
@21:15:12.00 Toroid.seek :crack
@21:15:13.51 Toroid.seek :cartridge
@21:15:14.60 Toroid.in :default
@21:15:14.66 Toroid.seek :clear
@21:15:16.62 Pressurizing cartridge to 14psi above 15.0psia
@21:15:16.63 SamplePump.setPosition! 0ml
@21:15:16.67 Intake.open
@21:15:17.38 Bypass.close
@21:15:18.10 Exhaust.close
@21:15:18.93 Pressure.in :default
@21:15:18.99 Pressure.hold
@21:15:19.16 SamplePump.in :charge
@21:15:19.22 SamplePump.seek 2.3ml,41 seconds
@21:15:29.68 Pressure.stop
@21:15:29.74 Intake.close
@21:15:30.54 Pressurized cartridge with 0.488ml to 14.2psi
@21:15:30.55 Isolated @25.5psia (10.4psi)
@21:15:30.57 Verifying cartridge holds at least 0.75..0.85 of 25.5psia
@21:15:30.60 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@21:15:35.17 Cartridge pressure fell 7% to 23.6psia
@21:15:35.25 Sampling 200.0ml
@21:15:35.26 SamplePump.setPosition! 0ml
@21:15:35.31 Exhaust.open
@21:15:36.03 Intake.open
@21:15:36.88 Pressure.in :default
@21:15:36.94 Pressure.hold
@21:15:37.16 SamplePump.in :sample
@21:15:37.23 SamplePump.seek 200ml,13:51
@21:15:39.92 <sampling> Cartridge 51 filtering 1/200ml at 0.31ml/s with 13.8psi vacuum
@21:16:39.92 Cartridge 51 filtering 21/200ml at 0.36ml/s with 11.5psi vacuum
@21:17:39.92 Cartridge 51 filtering 42/200ml at 0.34ml/s with 11.3psi vacuum
@21:18:39.92 Cartridge 51 filtering 62/200ml at 0.33ml/s with 11.5psi vacuum
@21:19:40.33 Cartridge 51 filtering 81/200ml at 0.32ml/s with 12.2psi vacuum 2.0m deep (17.6psia)
@21:20:39.92 Cartridge 51 filtering 101/200ml at 0.32ml/s with 16.5psi vacuum
@21:21:39.92 Cartridge 51 filtering 120/200ml at 0.31ml/s with 11.2psi vacuum
@21:22:39.92 Cartridge 51 filtering 138/200ml at 0.33ml/s with 14.7psi vacuum
@21:23:39.92 Cartridge 51 filtering 157/200ml at 0.28ml/s with 14.2psi vacuum
@21:24:39.92 Cartridge 51 filtering 175/200ml at 0.31ml/s with 15.7psi vacuum
@21:25:39.92 Cartridge 51 filtering 192/200ml at 0.31ml/s with 14.2psi vacuum
@21:26:08.64 <FILTERING> Pressure.stop
@21:26:08.76 <closeIntake> Intake.close
@21:26:08.81 <closeExhaust> Exhaust.close
@21:26:08.96 <FILTERING> Toroid.in :hold
@21:26:09.03 Toroid.seek :bypass
@21:26:10.64 Toroid.in :default
@21:26:10.70 Toroid.seek :clear
@21:26:12.32 Sampled  200.0ml
@21:26:13.72 <LRAUV> -> Cmd.startProcessing
@21:26:15.25 <PROCESSING> SPR.command \"read_sequences\"
@21:26:15.44 SPR.command \"stats_on\"
@21:26:15.48 SPR.command \"start 0 1\"
@21:26:16.29 <SPRlogger> SPR.start /var/log/esp/spr21aug0521h.spr.bz2
@21:26:18.29 <PROCESSING> Handoff.select :waste
@21:26:24.97 Syringe tare is 19% of full scale
@21:26:25.00 PinPort.in :locking
@21:26:25.07 PinPort.seek :locked
@21:26:25.55 <posArchive> Archive.in :fast
@21:26:25.61 <posLyS2> Lysis2.in :fast
@21:26:25.65 <PROCESSING> Lysis1.seek 1350uL
@21:26:25.75 <posArchive> Archive.seek :full
@21:26:25.85 <posLyS2> Lysis2.seek :full
@21:26:27.90 <PROCESSING> Plunge.seek :reagent
@21:26:28.80 Evacuating puck with filter bubble point of 24psi
@21:26:28.82 PinPort.seek :locked
@21:26:28.91 Handoff.select :waste
@21:26:35.49 Air syringe pumping up to 1 stroke to reach 8psi
@21:26:35.50 Air stroke #1 of 1
@21:26:35.59 Air.in :pressurize
@21:26:35.65 Air.seek :pressurized
@21:26:46.90 1.2psi after pressurize
@21:26:46.92 Air.in :compress
@21:26:46.99 Air.seek :evac
@21:26:51.07 1.2psi after compress
@21:27:03.64 0.5psi after compress (stable)
@21:27:03.66 Air.in :default
@21:27:03.73 Air.hold
@21:27:03.90 at 0.5psi Opened to waste
@21:27:03.91 Handoff.select :blocked
@21:27:04.05 Archive syringe pumping up to 8 strokes to reach 24psi
@21:27:04.06 Archive stroke #1 of 8
@21:27:04.14 Archive.in :pressurize
@21:27:04.23 Archive.seek :pressurized
@21:27:16.97 2.3psi after pressurize
@21:27:16.98 Archive.in :compress
@21:27:17.05 Archive.seek :empty
@21:27:32.93 15.1psi after compress
@21:27:45.50 15.8psi after compress (stable)
@21:27:45.52 Archive.in :gulp
@21:27:45.59 Archive.seek :topVent
@21:27:52.78 15.8psi after gulp
@21:28:05.35 15.8psi after gulp (stable)
@21:28:05.36 Archive stroke #2 of 8
@21:28:05.45 Archive.in :pressurize
@21:28:05.51 Archive.seek :pressurized
@21:28:18.24 15.8psi after pressurize
@21:28:18.26 Archive.in :compress
@21:28:18.33 Archive.seek :empty
@21:28:28.71 Reached 24.1 of 24.0psi after pumping 3343uL (1.93 strokes)
@21:28:28.72 Archive.in :default
@21:28:28.80 Archive.hold
@21:28:28.87 Handoff.select :waste
@21:28:39.07 delay 1:00
@21:29:39.09 Handoff.select :blocked
@21:30:01.78 Handoff.select :waste
@21:30:01.89 0.3psi at evacEnd
@21:30:01.90 Handoff.select :blocked
@21:30:02.03 Archive.in :fast
@21:30:02.10 Archive.seek :topVent
@21:30:15.40 Stable @0.3psi after Handoff blocked
@21:30:15.43 Archive syringe pumping up to 8 strokes to reach 15psi
@21:30:15.44 Archive stroke #1 of 8
@21:30:15.53 Archive.in :pressurize
@21:30:15.59 Archive.seek :pressurized
@21:30:28.31 0.7psi after pressurize
@21:30:28.32 Archive.in :compress
@21:30:28.40 Archive.seek :empty
@21:30:44.27 13.6psi after compress
@21:30:56.85 14.1psi after compress (stable)
@21:30:56.88 Archive.in :gulp
@21:30:56.96 Archive.seek :topVent
@21:31:04.15 14.1psi after gulp
@21:31:16.74 14.1psi after gulp (stable)
@21:31:16.76 Archive stroke #2 of 8
@21:31:16.86 Archive.in :pressurize
@21:31:16.92 Archive.seek :pressurized
@21:31:29.65 14.0psi after pressurize
@21:31:29.67 Archive.in :compress
@21:31:29.74 Archive.seek :empty
@21:31:33.09 Reached 15.4 of 15.3psi after pumping 3192uL (1.85 strokes)
@21:31:33.10 Archive.in :default
@21:31:33.17 Archive.hold
@21:31:33.23 Handoff.select :waste
@21:31:43.91 delay 1:00
@21:32:43.93 Handoff.select :blocked
@21:33:06.61 Handoff.select :waste
@21:33:06.73 0.4psi at evacEnd
@21:33:06.74 Air.seek :fullVent
@21:33:08.19 Archive.in :fast
@21:33:08.25 Archive.seek :topVent
@21:33:14.46 Seawater evacuated, Presure above/below filter released. Lysis begins
@21:33:14.55 Lysis1.in :slow
@21:33:14.61 Lysis1.seek 1050uL
@21:33:44.63 delay 5 seconds
@21:33:49.65 Handoff.select :blocked
@21:33:49.83 Lysis1.seek 450uL
@21:34:49.77 delay 3 seconds
@21:34:52.84 Air.in to mix into 1.37806 parts Lysis1
@21:34:53.42 <Airmixer> Air.seek :evac
@21:34:53.45 <PROCESSING> Lysis1.seek 300uL
@21:35:08.56 Lysis1.seek :empty
@21:35:38.65 Lysis buffer delivered @22.3psi
@21:35:38.66 Polling Can every 2:00
@21:35:39.21 PuckHeater.configure :default #ambient 25.4C
@21:35:39.26 PuckHeater.seek 79.6C
@21:36:28.82 PuckHeater.hold 2:00,79.6C
@21:38:28.91 Polling Can every 2:00
@21:38:29.46 PuckHeater.configure :default #ambient 25.4C
@21:38:29.51 PuckHeater.hold 30 seconds,78.6C
@21:38:59.70 Polling Can every 2:00
@21:39:00.25 PuckHeater.configure :default #ambient 25.4C
@21:39:00.30 PuckHeater.hold 30 seconds,78.1C
@21:39:30.50 Polling Can every 2:00
@21:39:31.05 PuckHeater.configure :default #ambient 25.4C
@21:39:31.10 PuckHeater.hold 10:00,77.5C
@21:49:31.35 Lysis complete. Syringe pressure @22.9psi
@21:49:31.36 Handoff.select :blocked
@21:49:31.41 Archive syringe pumping up to 5 strokes to reach 40psi
@21:49:31.42 Archive stroke #1 of 5
@21:49:31.50 Archive.in :pressurize
@21:49:31.57 Archive.seek :pressurized
@21:49:44.30 22.7psi after pressurize
@21:49:44.33 Archive.in :compress
@21:49:44.41 Archive.seek :empty
@21:49:49.67 Reached 40.3 of 40.0psi after pumping 1503uL (0.87 strokes)
@21:49:49.68 Archive.in :default
@21:49:49.75 Archive.hold
@21:49:49.82 delay 2 seconds
@21:49:51.84 Archive.in :fast
@21:49:51.92 Archive.seek :full
@21:49:58.34 Lysis1.seek 138uL
@21:50:12.21 delay 1 second
@21:50:13.24 <fillArchive> Archive.seek :full
@21:50:13.26 <PROCESSING> Lysis1.seek :empty
@21:50:27.24 Initial air pressure @46.1psi
@21:50:27.30 Lysis1.in :extract
@21:50:27.74 Lysis1.seek 600uL,nil
@21:50:32.94 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@21:50:32.95 Archive stroke #1 of 6
@21:50:33.03 Archive.in :pressurize
@21:50:33.10 Archive.seek :pressurized
@21:50:45.93 15.4psi after pressurize
@21:50:45.95 Archive.in :compress
@21:50:46.03 Archive.seek :empty
@21:50:50.39 Syringe::LysatePlungerStuck in PROCESSING -- at < 461uL during Lysis Syringe #1 (LyS1) move
@21:50:50.42 <PROCESSING> Lysis1.in :default
@21:50:50.49 Lysis1.seek 426uL
@21:50:52.17 <pump> Reached 25.2 of 25.0psi after pumping 1522uL (0.88 strokes)
@21:50:52.19 Archive.in :default
@21:50:52.25 Archive.hold
@21:50:52.45 <PROCESSING> Lysis1.in :extract
@21:50:52.55 Lysis1.seek 600uL,nil
@21:50:55.88 Initial 600uL lysate @20.8psi
@21:50:55.89 Handoff.select :blocked
@21:50:55.98 Initial air pressure @21.1psi
@21:50:56.05 Lysis1.seek 825uL,nil
@21:51:00.05 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@21:51:00.06 Archive stroke #1 of 6
@21:51:00.15 Archive.in :compress
@21:51:00.23 Archive.seek :empty
@21:51:00.34 Archive.in :default
@21:51:00.39 Archive.hold
@21:51:00.66 <PROCESSING> Recovered 825uL lysate @16.2psi
@21:51:00.67 Air.in :halfSpeed
@21:51:00.75 Air.seek :fullVent
@21:51:02.99 Puck Top Vented, bottom @7.7psi
@21:51:03.00 Handoff.select :waste
@21:51:03.12 delay 5 seconds
@21:51:08.19 Handoff open to waste, bottom @0.2psi
@21:51:08.21 Air.in :default
@21:51:08.28 Air.seek :evac
@21:51:09.72 Lysis2.in :DAmix
@21:51:09.83 Lysis1.in :DAmix
@21:51:10.35 <Lysis2mixer> Lysis2.seek 1480uL
@21:51:10.40 <PROCESSING> Lysis1.seek 575uL
@21:51:45.49 delay 5 seconds
@21:51:50.51 Handoff.select SPR
@21:51:50.63 SPR.command \"start 2 14\"
@21:51:51.52 SPR stabilized
@21:51:51.65 delay 10 seconds
@21:52:01.67 SPR.command \"start 2 0\"
@21:52:04.89 Waiting up to 11:00 for SPR to enter state 1
@21:54:02.55 SPR.command \"hold_on\"
@21:54:03.94 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2
@21:54:04.37 <Lysis2mixer> Lysis2.seek 1180uL
@21:54:04.44 <PROCESSING> Lysis1.seek 275uL
@21:54:46.46 delay 15 seconds
@21:55:01.48 SPR.command \"hold_off\"
@21:55:05.11 Waiting up to 38:00 for SPR to enter state 7
@22:19:02.69 SPR analysis completed
@22:19:06.92 <SPRlogger> Ended log spr21aug0521h.spr.bz2
@22:19:06.93 <PROCESSING> SPR.command \"stats_off\"
@22:19:08.30 <LRAUV> -> Cmd.stop
@22:19:08.32 Archive.stop
@22:19:08.45 <closeHandoff> Handoff.select :close
@22:19:08.58 <LRAUV> Gate.power :heat,:OFF
@22:19:08.60 Gate.power :cartridge,:OFF
@22:19:08.69 Carousel.seek 51
@22:19:08.86 Plunge.seek :locked
@22:19:09.75 PinPort.in :unlocking
@22:19:09.82 PinPort.seek :clear
@22:19:10.36 <Archive> Archive.in :unlocking
@22:19:10.49 <Air> Air.in :unlocking
@22:19:10.53 <Archive> Archive.seek :clear
@22:19:10.64 <Air> Air.seek :clear
@22:19:10.75 <Lysis1> Lysis1.in :unlocking
@22:19:10.88 <Lysis2> Lysis2.in :unlocking
@22:19:10.92 <Lysis1> Lysis1.seek :clear
@22:19:11.02 <Lysis2> Lysis2.seek :clear
@22:19:14.63 <LRAUV> Gate.power :motor,:OFF
@22:19:14.64 Gate.power :core,:OFF
@22:19:14.66 Gate.power :analytic1,:OFF
@22:19:14.69 Gate.power :analytic2,:OFF
@22:19:14.71 Gate.power :analytic3,:OFF
@22:19:14.73 Gate.power :main,:OFF
@22:19:14.75 Safely stopped and ready to power off
##-- file closed 2021-08-06T03:10:06.943Z