##-- file opened 2021-08-05T04:59:56.677Z
@00:59:56.99 -> showStatus
@00:59:58.61 -> Cmd.loadCartridge -18
@00:59:58.66 Gate.power :main,:ON,limit to 5000mA
@00:59:58.68 Gate.power :analytic1,:ON,limit to 5000mA
@00:59:58.71 Gate.power :analytic2,:ON,limit to 5000mA
@00:59:58.73 Gate.power :analytic3,:ON,limit to 5000mA
@00:59:58.87 Powering core bus
@01:00:01.15 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@01:00:01.61 <LRAUV> Archive.configure :default
@01:00:01.73 Air.configure :default
@01:00:01.83 Lysis1.configure :default
@01:00:01.94 Lysis2.configure :default
@01:00:02.07 Plunge.configure :default
@01:00:02.19 SamplePump.configure :default
@01:00:02.34 ExtraPump.configure :default
@01:00:02.44 Toroid.configure :default
@01:00:02.57 Carousel.configure :default
@01:00:02.70 Pressure.configure :default
@01:00:02.82 PinPort.configure :default
@01:00:02.87 All elf controllers running firmware version 4.741
@01:00:02.90 Gate.power :motor,:ON,limit to 5000mA
@01:00:03.25 <Archive_home> Archive.in :default
@01:00:03.33 <Air_home> Air.in :default
@01:00:03.40 <Lysis1_home> Lysis1.in :default
@01:00:03.46 <Lysis2_home> Lysis2.in :default
@01:00:03.56 <Plunge_home> Plunge.in :default
@01:00:03.67 <Toroid_home> Toroid.in :default
@01:00:03.74 <Air_home> Air.home.jog 0
@01:00:03.77 <Archive_home> Archive.home.jog 0
@01:00:03.80 <PinPort_home> PinPort.in :default
@01:00:03.83 <Lysis2_home> Lysis2.home.jog 0
@01:00:03.85 <Lysis1_home> Lysis1.home.jog 0
@01:00:03.92 <Plunge_home> Plunge.home.jog 0
@01:00:04.04 <Toroid_home> Toroid.home.jog 0
@01:00:04.14 <PinPort_home> PinPort.home.jog 0
@01:00:04.60 <Plunge_home> Plunge.in :homing
@01:00:04.67 <Air_home> Air.in :homing
@01:00:04.70 <Lysis1_home> Lysis1.in :homing
@01:00:04.77 <Archive_home> Archive.in :homing
@01:00:04.80 <Lysis2_home> Lysis2.in :homing
@01:00:04.97 <Plunge_home> Plunge.jog 105
@01:00:05.02 <Air_home> Air.jog 1415
@01:00:05.04 <Archive_home> Archive.jog 1401
@01:00:05.07 <Lysis1_home> Lysis1.jog 1411
@01:00:05.10 <Lysis2_home> Lysis2.jog 1418
@01:00:05.35 <Toroid_home> Toroid.in :homing
@01:00:05.37 <PinPort_home> PinPort.in :homing
@01:00:05.48 PinPort.jog -109
@01:00:05.50 <Toroid_home> Toroid.jog 619
@01:00:05.62 <Plunge_home> Plunge.setRawPosition! 50
@01:00:05.66 Plunge.seek :home
@01:00:06.13 <PinPort_home> PinPort.setRawPosition! 0
@01:00:06.15 <Plunge_home> Plunge.in :default
@01:00:06.21 <PinPort_home> PinPort.seek :home
@01:00:06.40 PinPort.in :default
@01:00:06.42 <Lysis2_home> Lysis2.setRawPosition! 50
@01:00:06.44 <Lysis1_home> Lysis1.setRawPosition! 50
@01:00:06.47 <Air_home> Air.setRawPosition! 50
@01:00:06.49 <Archive_home> Archive.setRawPosition! 50
@01:00:06.61 <Lysis2_home> Lysis2.seek :home
@01:00:06.71 <Air_home> Air.seek :home
@01:00:06.74 <Lysis1_home> Lysis1.seek :home
@01:00:06.79 <Archive_home> Archive.seek :home
@01:00:07.05 <Lysis2_home> Lysis2.in :default
@01:00:07.16 <Air_home> Air.in :default
@01:00:07.19 <Lysis1_home> Lysis1.in :default
@01:00:07.31 <Archive_home> Archive.in :default
@01:00:07.65 <Toroid_home> Toroid.setRawPosition! 0
@01:00:07.69 Toroid.seek :home
@01:00:07.79 Toroid.in :default
@01:00:07.91 <LRAUV> Toroid.seek :clear
@01:00:09.52 Carousel.in :default
@01:00:09.58 Carousel.home.jog 0
@01:00:10.07 Carousel.in :default
@01:00:10.13 Carousel.seek 1
@01:00:10.28 Selecting Cartridge 60
@01:00:10.53 Carousel.seek 60
@01:00:22.84 Lysis1.in :fast
@01:00:22.90 Lysis1.seek :locked
@01:00:25.50 <Plunge> Plunge.seek :locked
@01:00:25.62 <Air> Air.in :fast
@01:00:25.68 Air.seek :locked
@01:00:28.28 <LRAUV> Powering cartridge bus
@01:00:30.90 Gate.setIR 1750,5000,0,6
@01:00:31.03 Gate.setIR 0,5000,0,6
@01:00:31.09 PuckHeater.configure :default
@01:00:31.15 Gate.power :heat,:ON,limit to 5000mA
@01:00:31.83 Mounted lysis Cartridge board#Q05034
@01:00:31.86 Ignoring this cartridge's HeatingAccelInCarousel heat loss curve
@01:00:39.07 -> Cmd.startFiltering
@01:00:39.52 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@01:00:39.53 SamplePump.setPosition! 0ml
@01:00:39.58 Bypass.open
@01:00:41.06 Intake.open
@01:00:41.85 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@01:00:44.42 Vacuum tare is 50% of full scale
@01:00:44.55 Pressure.in :default
@01:00:44.61 Pressure.hold
@01:00:44.78 SamplePump.in :prime
@01:00:44.84 SamplePump.seek 10ml,59 seconds
@01:00:54.10 Pressure.stop
@01:00:54.15 SamplePump.setPosition! 0ml
@01:00:54.20 Exhaust.open
@01:00:55.59 <closeBypass> Bypass.close
@01:00:57.11 <FILTERING> Pressure.in :default
@01:00:57.17 Pressure.hold
@01:00:57.39 SamplePump.in :prime
@01:00:57.46 SamplePump.seek 100ml,5:16
@01:02:53.60 Pressure.stop
@01:02:53.66 Bypass.open
@01:02:54.39 Intake.close
@01:02:55.18 Toroid.in :hold
@01:02:55.24 Toroid.seek :crack
@01:02:56.76 Toroid.seek :cartridge
@01:02:57.84 Toroid.in :default
@01:02:57.90 Toroid.seek :clear
@01:02:59.86 Pressurizing cartridge to 14psi above 13.7psia
@01:02:59.87 SamplePump.setPosition! 0ml
@01:02:59.91 Intake.open
@01:03:00.61 Bypass.close
@01:03:01.35 Exhaust.close
@01:03:02.13 Pressure.hold
@01:03:02.31 SamplePump.in :charge
@01:03:02.37 SamplePump.seek 2.3ml,41 seconds
@01:03:14.85 Pressure.stop
@01:03:14.90 Intake.close
@01:03:15.69 Pressurized cartridge with 0.694ml to 14.0psi
@01:03:15.70 Isolated @24.1psia (10.4psi)
@01:03:15.72 Verifying cartridge holds at least 0.75..0.85 of 24.1psia
@01:03:15.75 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@01:03:20.37 Sampling 75.0ml
@01:03:20.39 SamplePump.setPosition! 0ml
@01:03:20.44 Exhaust.open
@01:03:21.14 Intake.open
@01:03:21.98 Pressure.in :default
@01:03:22.04 Pressure.hold
@01:03:22.26 SamplePump.in :sample
@01:03:22.33 SamplePump.seek 75ml,5:31
@01:03:25.00 <sampling> Cartridge 60 filtering 1/75ml at 0.38ml/s with 13.8psi vacuum
@01:04:25.00 Cartridge 60 filtering 22/75ml at 0.39ml/s with 12.0psi vacuum
@01:05:25.01 Cartridge 60 filtering 44/75ml at 0.38ml/s with 9.0psi vacuum
@01:06:25.00 Cartridge 60 filtering 65/75ml at 0.33ml/s with 15.8psi vacuum
@01:06:52.31 <FILTERING> Pressure.stop
@01:06:52.43 <closeIntake> Intake.close
@01:06:52.48 <closeExhaust> Exhaust.close
@01:06:52.62 <FILTERING> Toroid.in :hold
@01:06:52.68 Toroid.seek :bypass
@01:06:54.30 Toroid.in :default
@01:06:54.36 Toroid.seek :clear
@01:06:55.98 Sampled  75.0ml
@01:06:57.27 <LRAUV> -> Cmd.startProcessing
@01:06:58.79 <PROCESSING> SPR.command \"read_sequences\"
@01:06:59.08 SPR.command \"stats_on\"
@01:06:59.12 SPR.command \"start 0 1\"
@01:06:59.93 <SPRlogger> SPR.start /var/log/esp/spr21aug0501h.spr.bz2
@01:07:02.44 <PROCESSING> Handoff.select :waste
@01:07:09.13 Syringe tare is 18% of full scale
@01:07:09.16 PinPort.in :locking
@01:07:09.23 PinPort.seek :locked
@01:07:09.70 <posArchive> Archive.in :fast
@01:07:09.74 <posLyS2> Lysis2.in :fast
@01:07:09.81 <PROCESSING> Lysis1.seek 1350uL
@01:07:09.87 <posArchive> Archive.seek :full
@01:07:09.94 <posLyS2> Lysis2.seek :full
@01:07:12.10 <PROCESSING> Plunge.seek :reagent
@01:07:13.01 Evacuating puck with filter bubble point of 24psi
@01:07:13.03 PinPort.seek :locked
@01:07:13.11 Handoff.select :waste
@01:07:19.69 Air syringe pumping up to 1 stroke to reach 8psi
@01:07:19.70 Air stroke #1 of 1
@01:07:19.79 Air.in :pressurize
@01:07:19.85 Air.seek :pressurized
@01:07:31.10 0.0psi after pressurize
@01:07:31.11 Air.in :compress
@01:07:31.19 Air.seek :evac
@01:07:35.28 2.6psi after compress
@01:07:47.87 0.4psi after compress (stable)
@01:07:47.89 Air.in :default
@01:07:47.96 Air.hold
@01:07:48.13 at 0.3psi Opened to waste
@01:07:48.14 Handoff.select :blocked
@01:07:48.27 Archive syringe pumping up to 8 strokes to reach 24psi
@01:07:48.28 Archive stroke #1 of 8
@01:07:48.35 Archive.in :pressurize
@01:07:48.42 Archive.seek :pressurized
@01:08:01.14 1.9psi after pressurize
@01:08:01.16 Archive.in :compress
@01:08:01.23 Archive.seek :empty
@01:08:17.11 15.2psi after compress
@01:08:29.70 16.2psi after compress (stable)
@01:08:29.72 Archive.in :gulp
@01:08:29.79 Archive.seek :topVent
@01:08:37.00 16.3psi after gulp
@01:08:49.58 16.3psi after gulp (stable)
@01:08:49.59 Archive stroke #2 of 8
@01:08:49.68 Archive.in :pressurize
@01:08:49.74 Archive.seek :pressurized
@01:09:02.48 16.1psi after pressurize
@01:09:02.50 Archive.in :compress
@01:09:02.59 Archive.seek :empty
@01:09:14.70 Reached 24.1 of 24.0psi after pumping 3380uL (1.95 strokes)
@01:09:14.71 Archive.in :default
@01:09:14.78 Archive.hold
@01:09:14.86 Handoff.select :waste
@01:09:24.57 delay 1:00
@01:10:24.59 Handoff.select :blocked
@01:10:47.27 Handoff.select :waste
@01:10:47.39 0.5psi at evacEnd
@01:10:47.40 Handoff.select :blocked
@01:10:47.53 Archive.in :fast
@01:10:47.61 Archive.seek :topVent
@01:11:01.03 Stable @0.4psi after Handoff blocked
@01:11:01.05 Archive syringe pumping up to 8 strokes to reach 15psi
@01:11:01.07 Archive stroke #1 of 8
@01:11:01.16 Archive.in :pressurize
@01:11:01.22 Archive.seek :pressurized
@01:11:13.96 0.5psi after pressurize
@01:11:13.98 Archive.in :compress
@01:11:14.07 Archive.seek :empty
@01:11:29.96 14.1psi after compress
@01:11:42.53 14.8psi after compress (stable)
@01:11:42.55 Archive.in :gulp
@01:11:42.62 Archive.seek :topVent
@01:11:49.83 14.9psi after gulp
@01:12:02.40 14.9psi after gulp (stable)
@01:12:02.41 Archive stroke #2 of 8
@01:12:02.50 Archive.in :pressurize
@01:12:02.56 Archive.seek :pressurized
@01:12:15.30 14.8psi after pressurize
@01:12:15.32 Archive.in :compress
@01:12:15.39 Archive.seek :empty
@01:12:22.29 Reached 15.6 of 15.4psi after pumping 3268uL (1.89 strokes)
@01:12:22.30 Archive.in :default
@01:12:22.37 Archive.hold
@01:12:22.43 Handoff.select :waste
@01:12:29.61 delay 1:00
@01:13:29.64 Handoff.select :blocked
@01:13:52.32 Handoff.select :waste
@01:13:52.44 0.6psi at evacEnd
@01:13:52.46 Air.seek :fullVent
@01:13:53.92 Archive.in :fast
@01:13:53.98 Archive.seek :topVent
@01:14:00.46 Seawater evacuated, Presure above/below filter released. Lysis begins
@01:14:00.57 Lysis1.in :slow
@01:14:00.63 Lysis1.seek 1050uL
@01:14:30.66 delay 5 seconds
@01:14:35.68 Handoff.select :blocked
@01:14:35.85 Lysis1.seek 450uL
@01:15:35.83 delay 3 seconds
@01:15:38.92 Air.in to mix into 1.37806 parts Lysis1
@01:15:39.43 <Airmixer> Air.seek :evac
@01:15:39.47 <PROCESSING> Lysis1.seek 300uL
@01:15:54.58 Lysis1.seek :empty
@01:16:24.68 Lysis buffer delivered @17.9psi
@01:16:24.69 Polling Can every 2:00
@01:16:25.24 PuckHeater.configure :default #ambient 23.2C
@01:16:25.29 PuckHeater.seek 79.7C
@01:17:16.93 PuckHeater.hold 2:00,79.7C
@01:19:17.15 Polling Can every 2:00
@01:19:17.70 PuckHeater.configure :default #ambient 23.3C
@01:19:17.75 PuckHeater.hold 30 seconds,78.6C
@01:19:47.98 Polling Can every 2:00
@01:19:48.54 PuckHeater.hold 30 seconds,78.1C
@01:20:18.81 Polling Can every 2:00
@01:20:19.37 PuckHeater.hold 10:00,77.6C
@01:30:19.96 Lysis complete. Syringe pressure @10.2psi
@01:30:19.97 Handoff.select :blocked
@01:30:20.01 Archive syringe pumping up to 5 strokes to reach 40psi
@01:30:20.02 Archive stroke #1 of 5
@01:30:20.11 Archive.in :pressurize
@01:30:20.17 Archive.seek :pressurized
@01:30:32.91 10.0psi after pressurize
@01:30:32.92 Archive.in :compress
@01:30:33.00 Archive.seek :empty
@01:30:43.21 Reached 40.4 of 40.0psi after pumping 1609uL (0.93 strokes)
@01:30:43.22 Archive.in :default
@01:30:43.28 Archive.hold
@01:30:43.36 delay 2 seconds
@01:30:45.38 Archive.in :fast
@01:30:45.44 Archive.seek :full
@01:30:52.22 Lysis1.seek 138uL
@01:31:06.08 delay 1 second
@01:31:07.11 <fillArchive> Archive.seek :full
@01:31:07.17 <PROCESSING> Lysis1.seek :empty
@01:31:21.13 Initial air pressure @45.2psi
@01:31:21.20 Lysis1.in :extract
@01:31:21.27 Lysis1.seek 600uL,nil
@01:31:27.50 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@01:31:27.52 Archive stroke #1 of 6
@01:31:27.62 Archive.in :pressurize
@01:31:27.69 Archive.seek :pressurized
@01:31:40.42 14.5psi after pressurize
@01:31:40.43 Archive.in :compress
@01:31:40.50 Archive.seek :empty
@01:31:47.96 Syringe::LysatePlungerStuck in PROCESSING -- at < 412uL during Lysis Syringe #1 (LyS1) move
@01:31:47.99 <PROCESSING> Lysis1.in :default
@01:31:48.06 Lysis1.seek 377uL
@01:31:48.99 <pump> Reached 25.7 of 25.0psi after pumping 1572uL (0.91 strokes)
@01:31:49.01 Archive.in :default
@01:31:49.07 Archive.hold
@01:31:49.86 <PROCESSING> Lysis1.in :extract
@01:31:49.94 Lysis1.seek 600uL,nil
@01:31:54.13 Initial 600uL lysate @21.6psi
@01:31:54.14 Handoff.select :blocked
@01:31:54.24 Initial air pressure @22.0psi
@01:31:54.30 Lysis1.seek 825uL,nil
@01:31:58.29 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@01:31:58.30 Archive stroke #1 of 6
@01:31:58.39 Archive.in :compress
@01:31:58.46 Archive.seek :empty
@01:32:00.31 Archive.in :default
@01:32:00.36 Archive.hold
@01:32:00.67 <PROCESSING> Recovered 825uL lysate @16.6psi
@01:32:00.69 Air.in :halfSpeed
@01:32:00.77 Air.seek :fullVent
@01:32:03.02 Puck Top Vented, bottom @3.0psi
@01:32:03.03 Handoff.select :waste
@01:32:03.15 delay 5 seconds
@01:32:08.22 Handoff open to waste, bottom @0.1psi
@01:32:08.25 Air.in :default
@01:32:08.31 Air.seek :evac
@01:32:09.75 Lysis2.in :DAmix
@01:32:09.85 Lysis1.in :DAmix
@01:32:10.43 <Lysis2mixer> Lysis2.seek 1480uL
@01:32:10.47 <PROCESSING> Lysis1.seek 575uL
@01:32:45.51 delay 5 seconds
@01:32:50.53 Handoff.select SPR
@01:32:50.64 SPR.command \"start 2 14\"
@01:32:51.75 SPR stabilized
@01:32:51.88 delay 10 seconds
@01:33:01.90 SPR.command \"start 2 0\"
@01:33:04.60 Waiting up to 11:00 for SPR to enter state 1
@01:35:02.27 SPR.command \"hold_on\"
@01:35:03.66 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2
@01:35:04.09 <Lysis2mixer> Lysis2.seek 1180uL
@01:35:04.13 <PROCESSING> Lysis1.seek 275uL
@01:35:46.16 delay 15 seconds
@01:36:01.18 SPR.command \"hold_off\"
@01:36:04.31 Waiting up to 38:00 for SPR to enter state 7
@02:00:01.46 SPR analysis completed
@02:00:05.61 <SPRlogger> Ended log spr21aug0501h.spr.bz2
@02:00:05.62 <PROCESSING> SPR.command \"stats_off\"
@02:00:07.03 <LRAUV> -> Cmd.stop
@02:00:07.04 Archive.stop
@02:00:07.16 <closeHandoff> Handoff.select :close
@02:00:07.29 <LRAUV> Gate.power :heat,:OFF
@02:00:07.31 Gate.power :cartridge,:OFF
@02:00:07.39 Carousel.seek 60
@02:00:07.57 Plunge.seek :locked
@02:00:08.45 PinPort.in :unlocking
@02:00:08.51 PinPort.seek :clear
@02:00:09.05 <Archive> Archive.in :unlocking
@02:00:09.18 <Air> Air.in :unlocking
@02:00:09.22 <Archive> Archive.seek :clear
@02:00:09.34 <Air> Air.seek :clear
@02:00:09.44 <Lysis1> Lysis1.in :unlocking
@02:00:09.57 Lysis1.seek :clear
@02:00:09.63 <Lysis2> Lysis2.in :unlocking
@02:00:09.71 Lysis2.seek :clear
@02:00:13.29 <LRAUV> Gate.power :motor,:OFF
@02:00:13.31 Gate.power :core,:OFF
@02:00:13.33 Gate.power :analytic1,:OFF
@02:00:13.35 Gate.power :analytic2,:OFF
@02:00:13.38 Gate.power :analytic3,:OFF
@02:00:13.40 Gate.power :main,:OFF
@02:00:13.42 Safely stopped and ready to power off
##-- file closed 2021-08-05T06:29:35.241Z

##-- file opened 2021-08-05T06:29:35.248Z
@02:29:35.74 -> showStatus
@02:29:37.73 -> Cmd.loadCartridge -18
@02:29:37.79 Gate.power :main,:ON,limit to 5000mA
@02:29:37.80 Gate.power :analytic1,:ON,limit to 5000mA
@02:29:37.82 Gate.power :analytic2,:ON,limit to 5000mA
@02:29:37.85 Gate.power :analytic3,:ON,limit to 5000mA
@02:29:38.01 Powering core bus
@02:29:40.26 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@02:29:40.76 <LRAUV> Archive.configure :default
@02:29:40.88 Air.configure :default
@02:29:40.99 Lysis1.configure :default
@02:29:41.09 Lysis2.configure :default
@02:29:41.22 Plunge.configure :default
@02:29:41.34 SamplePump.configure :default
@02:29:41.48 ExtraPump.configure :default
@02:29:41.60 Toroid.configure :default
@02:29:41.72 Carousel.configure :default
@02:29:41.84 Pressure.configure :default
@02:29:41.95 PinPort.configure :default
@02:29:42.01 All elf controllers running firmware version 4.741
@02:29:42.04 Gate.power :motor,:ON,limit to 5000mA
@02:29:42.18 <Archive_home> Archive.in :default
@02:29:42.51 Archive.home.jog 0
@02:29:42.68 <PinPort_home> PinPort.in :default
@02:29:42.73 <Toroid_home> Toroid.in :default
@02:29:42.76 <Lysis2_home> Lysis2.in :default
@02:29:42.80 <Air_home> Air.in :default
@02:29:42.84 <Plunge_home> Plunge.in :default
@02:29:42.87 <Lysis1_home> Lysis1.in :default
@02:29:43.07 <PinPort_home> PinPort.home.jog 0
@02:29:43.10 <Toroid_home> Toroid.home.jog 0
@02:29:43.12 <Lysis2_home> Lysis2.home.jog 0
@02:29:43.15 <Plunge_home> Plunge.home.jog 0
@02:29:43.17 <Air_home> Air.home.jog 0
@02:29:43.21 <Lysis1_home> Lysis1.home.jog 0
@02:29:43.75 <Toroid_home> Toroid.in :homing
@02:29:43.79 <Plunge_home> Plunge.in :homing
@02:29:43.87 <Archive_home> Archive.in :homing
@02:29:43.89 <PinPort_home> PinPort.in :homing
@02:29:43.95 <Lysis2_home> Lysis2.in :homing
@02:29:44.12 <Plunge_home> Plunge.jog 1563
@02:29:44.17 <Toroid_home> Toroid.jog 620
@02:29:44.19 <Archive_home> Archive.jog 1403
@02:29:44.22 <PinPort_home> PinPort.jog -109
@02:29:44.25 <Lysis2_home> Lysis2.jog 1419
@02:29:44.49 <Air_home> Air.in :homing
@02:29:44.51 <Lysis1_home> Lysis1.in :homing
@02:29:44.62 Lysis1.jog 1414
@02:29:44.64 <Air_home> Air.jog 154
@02:29:44.76 <PinPort_home> PinPort.setRawPosition! 0
@02:29:45.18 PinPort.seek :home
@02:29:45.27 <Air_home> Air.setRawPosition! 50
@02:29:45.29 <PinPort_home> PinPort.in :default
@02:29:45.36 <Air_home> Air.seek :home
@02:29:45.49 <Plunge_home> Plunge.setRawPosition! 50
@02:29:45.50 <Archive_home> Archive.setRawPosition! 50
@02:29:45.58 <Plunge_home> Plunge.seek :home
@02:29:45.62 <Archive_home> Archive.seek :home
@02:29:45.78 <Lysis2_home> Lysis2.setRawPosition! 50
@02:29:45.80 <Air_home> Air.in :default
@02:29:45.89 <Lysis2_home> Lysis2.seek :home
@02:29:46.04 <Lysis1_home> Lysis1.setRawPosition! 50
@02:29:46.07 <Plunge_home> Plunge.in :default
@02:29:46.10 <Archive_home> Archive.in :default
@02:29:46.17 <Lysis1_home> Lysis1.seek :home
@02:29:46.33 <Lysis2_home> Lysis2.in :default
@02:29:46.42 <Toroid_home> Toroid.setRawPosition! 0
@02:29:46.47 Toroid.seek :home
@02:29:46.58 Toroid.in :default
@02:29:46.61 <Lysis1_home> Lysis1.in :default
@02:29:47.02 <LRAUV> Toroid.seek :clear
@02:29:48.64 Carousel.in :default
@02:29:48.71 Carousel.home.jog 0
@02:29:49.20 Carousel.in :default
@02:29:49.26 Carousel.seek 60
@02:29:49.42 Selecting Cartridge 59
@02:29:49.66 Carousel.seek 59
@02:29:50.14 Lysis1.in :fast
@02:29:50.20 Lysis1.seek :locked
@02:29:52.79 <Plunge> Plunge.seek :locked
@02:29:52.91 <Air> Air.in :fast
@02:29:52.97 Air.seek :locked
@02:29:55.57 <LRAUV> Powering cartridge bus
@02:29:58.19 Gate.setIR 1750,5000,0,6
@02:29:58.32 Gate.setIR 0,5000,0,6
@02:29:58.38 PuckHeater.configure :default
@02:29:58.43 Gate.power :heat,:ON,limit to 5000mA
@02:29:59.11 Mounted lysis Cartridge board#Q05046
@02:29:59.14 Using default puck heat loss curve for HeatingAccelInCarousel
@02:30:18.13 -> Cmd.startFiltering
@02:30:18.59 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@02:30:18.60 SamplePump.setPosition! 0ml
@02:30:18.64 Bypass.open
@02:30:20.12 Intake.open
@02:30:20.90 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@02:30:23.47 Vacuum tare is 50% of full scale
@02:30:23.59 Pressure.in :default
@02:30:23.65 Pressure.hold
@02:30:23.82 SamplePump.in :prime
@02:30:23.89 SamplePump.seek 10ml,59 seconds
@02:30:33.15 Pressure.stop
@02:30:33.21 SamplePump.setPosition! 0ml
@02:30:33.25 Exhaust.open
@02:30:34.71 <closeBypass> Bypass.close
@02:30:36.25 <FILTERING> Pressure.in :default
@02:30:36.31 Pressure.hold
@02:30:36.52 SamplePump.in :prime
@02:30:36.59 SamplePump.seek 100ml,5:16
@02:32:31.10 Pressure.stop
@02:32:31.16 Bypass.open
@02:32:31.89 Intake.close
@02:32:32.70 Toroid.in :hold
@02:32:32.76 Toroid.seek :crack
@02:32:34.28 Toroid.seek :cartridge
@02:32:35.36 Toroid.in :default
@02:32:35.42 Toroid.seek :clear
@02:32:37.38 Pressurizing cartridge to 14psi above 13.4psia
@02:32:37.39 SamplePump.setPosition! 0ml
@02:32:37.44 Intake.open
@02:32:38.15 Bypass.close
@02:32:38.86 Exhaust.close
@02:32:39.70 Pressure.in :default
@02:32:39.76 Pressure.hold
@02:32:39.93 SamplePump.in :charge
@02:32:40.00 SamplePump.seek 2.3ml,41 seconds
@02:32:52.57 Pressure.stop
@02:32:52.62 Intake.close
@02:32:53.41 Pressurized cartridge with 0.707ml to 14.0psi
@02:32:53.42 Isolated @23.8psia (10.4psi)
@02:32:53.44 Verifying cartridge holds at least 0.75..0.85 of 23.8psia
@02:32:53.47 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@02:32:58.05 Cartridge pressure fell 8% to 21.9psia
@02:32:58.12 Sampling 75.0ml
@02:32:58.14 SamplePump.setPosition! 0ml
@02:32:58.19 Exhaust.open
@02:32:58.92 Intake.open
@02:32:59.76 Pressure.in :default
@02:32:59.82 Pressure.hold
@02:33:00.05 SamplePump.in :sample
@02:33:00.12 SamplePump.seek 75ml,5:31
@02:33:02.79 <sampling> Cartridge 59 filtering 1/75ml at 0.39ml/s with 14.3psi vacuum
@02:34:02.80 Cartridge 59 filtering 23/75ml at 0.37ml/s with 11.7psi vacuum
@02:35:02.80 Cartridge 59 filtering 45/75ml at 0.39ml/s with 12.7psi vacuum
@02:36:02.80 Cartridge 59 filtering 67/75ml at 0.35ml/s with 11.5psi vacuum
@02:36:26.06 <FILTERING> Pressure.stop
@02:36:26.18 <closeIntake> Intake.close
@02:36:26.22 <closeExhaust> Exhaust.close
@02:36:26.38 <FILTERING> Toroid.in :hold
@02:36:26.44 Toroid.seek :bypass
@02:36:28.06 Toroid.in :default
@02:36:28.12 Toroid.seek :clear
@02:36:29.73 Sampled  75.0ml
@02:36:31.26 <LRAUV> -> Cmd.startProcessing
@02:36:32.78 <PROCESSING> SPR.command \"read_sequences\"
@02:36:33.47 SPR.command \"stats_on\"
@02:36:33.51 SPR.command \"start 0 1\"
@02:36:34.32 <SPRlogger> SPR.start /var/log/esp/spr21aug0502h.spr.bz2
@02:36:36.31 <PROCESSING> Handoff.select :waste
@02:36:43.00 Syringe tare is 18% of full scale
@02:36:43.02 PinPort.in :locking
@02:36:43.09 PinPort.seek :locked
@02:36:43.57 <posArchive> Archive.in :fast
@02:36:43.61 <posLyS2> Lysis2.in :fast
@02:36:43.66 <PROCESSING> Lysis1.seek 1350uL
@02:36:43.79 <posArchive> Archive.seek :full
@02:36:43.84 <posLyS2> Lysis2.seek :full
@02:36:45.91 <PROCESSING> Plunge.seek :reagent
@02:36:46.82 Evacuating puck with filter bubble point of 24psi
@02:36:46.84 PinPort.seek :locked
@02:36:46.93 Handoff.select :waste
@02:36:53.51 Air syringe pumping up to 1 stroke to reach 8psi
@02:36:53.52 Air stroke #1 of 1
@02:36:53.60 Air.in :pressurize
@02:36:53.66 Air.seek :pressurized
@02:37:04.91 0.3psi after pressurize
@02:37:04.92 Air.in :compress
@02:37:05.00 Air.seek :evac
@02:37:09.09 1.0psi after compress
@02:37:21.66 0.4psi after compress (stable)
@02:37:21.68 Air.in :default
@02:37:21.75 Air.hold
@02:37:21.92 at 0.4psi Opened to waste
@02:37:21.93 Handoff.select :blocked
@02:37:22.06 Archive syringe pumping up to 8 strokes to reach 24psi
@02:37:22.07 Archive stroke #1 of 8
@02:37:22.15 Archive.in :pressurize
@02:37:22.23 Archive.seek :pressurized
@02:37:34.96 0.9psi after pressurize
@02:37:34.97 Archive.in :compress
@02:37:35.05 Archive.seek :empty
@02:37:50.92 14.3psi after compress
@02:38:03.50 15.3psi after compress (stable)
@02:38:03.51 Archive.in :gulp
@02:38:03.58 Archive.seek :topVent
@02:38:10.77 15.4psi after gulp
@02:38:23.34 15.4psi after gulp (stable)
@02:38:23.35 Archive stroke #2 of 8
@02:38:23.44 Archive.in :pressurize
@02:38:23.50 Archive.seek :pressurized
@02:38:36.22 15.3psi after pressurize
@02:38:36.24 Archive.in :compress
@02:38:36.31 Archive.seek :empty
@02:38:49.02 Reached 24.1 of 24.0psi after pumping 3393uL (1.96 strokes)
@02:38:49.03 Archive.in :default
@02:38:49.10 Archive.hold
@02:38:49.16 Handoff.select :waste
@02:38:58.37 delay 1:00
@02:39:58.40 Handoff.select :blocked
@02:40:21.08 Handoff.select :waste
@02:40:21.20 0.5psi at evacEnd
@02:40:21.21 Handoff.select :blocked
@02:40:21.34 Archive.in :fast
@02:40:21.41 Archive.seek :topVent
@02:40:34.88 Stable @0.5psi after Handoff blocked
@02:40:34.90 Archive syringe pumping up to 8 strokes to reach 16psi
@02:40:34.91 Archive stroke #1 of 8
@02:40:35.00 Archive.in :pressurize
@02:40:35.06 Archive.seek :pressurized
@02:40:47.79 0.6psi after pressurize
@02:40:47.80 Archive.in :compress
@02:40:47.88 Archive.seek :empty
@02:41:03.75 13.5psi after compress
@02:41:16.32 14.2psi after compress (stable)
@02:41:16.34 Archive.in :gulp
@02:41:16.41 Archive.seek :topVent
@02:41:23.61 14.3psi after gulp
@02:41:36.18 14.2psi after gulp (stable)
@02:41:36.19 Archive stroke #2 of 8
@02:41:36.28 Archive.in :pressurize
@02:41:36.34 Archive.seek :pressurized
@02:41:49.06 14.2psi after pressurize
@02:41:49.07 Archive.in :compress
@02:41:49.15 Archive.seek :empty
@02:41:54.11 Reached 15.6 of 15.5psi after pumping 3226uL (1.86 strokes)
@02:41:54.12 Archive.in :default
@02:41:54.19 Archive.hold
@02:41:54.26 Handoff.select :waste
@02:42:01.43 delay 1:00
@02:43:01.46 Handoff.select :blocked
@02:43:24.13 Handoff.select :waste
@02:43:24.25 0.4psi at evacEnd
@02:43:24.27 Air.seek :fullVent
@02:43:25.71 Archive.in :fast
@02:43:25.77 Archive.seek :topVent
@02:43:32.10 Seawater evacuated, Presure above/below filter released. Lysis begins
@02:43:32.19 Lysis1.in :slow
@02:43:32.25 Lysis1.seek 1050uL
@02:44:02.26 delay 5 seconds
@02:44:07.28 Handoff.select :blocked
@02:44:07.45 Lysis1.seek 450uL
@02:45:07.40 delay 3 seconds
@02:45:10.47 Air.in to mix into 1.37806 parts Lysis1
@02:45:10.98 <Airmixer> Air.seek :evac
@02:45:11.02 <PROCESSING> Lysis1.seek 300uL
@02:45:26.12 Lysis1.seek :empty
@02:45:56.21 Lysis buffer delivered @14.1psi
@02:45:56.22 Polling Can every 2:00
@02:45:56.77 PuckHeater.configure :default #ambient 23.5C
@02:45:56.84 PuckHeater.seek 79.7C
@02:46:44.25 PuckHeater.hold 2:00,79.7C
@02:48:44.23 Polling Can every 2:00
@02:48:44.78 PuckHeater.configure :default #ambient 23.5C
@02:48:44.83 PuckHeater.hold 30 seconds,78.6C
@02:49:15.00 Polling Can every 2:00
@02:49:15.54 PuckHeater.configure :default #ambient 23.6C
@02:49:15.59 PuckHeater.hold 30 seconds,78.1C
@02:49:45.76 Polling Can every 2:00
@02:49:46.30 PuckHeater.configure :default #ambient 23.6C
@02:49:46.35 PuckHeater.hold 10:00,77.6C
@02:59:45.99 Lysis complete. Syringe pressure @7.5psi
@02:59:46.00 Handoff.select :blocked
@02:59:46.05 Archive syringe pumping up to 5 strokes to reach 40psi
Archive stroke #1 of 5
@02:59:46.14 Archive.in :pressurize
@02:59:46.21 Archive.seek :pressurized
@02:59:58.95 7.4psi after pressurize
@02:59:58.96 Archive.in :compress
@02:59:59.04 Archive.seek :empty
@03:00:11.04 Reached 40.4 of 40.0psi after pumping 1647uL (0.95 strokes)
@03:00:11.05 Archive.in :default
@03:00:11.11 Archive.hold
@03:00:11.18 delay 2 seconds
@03:00:13.20 Archive.in :fast
@03:00:13.26 Archive.seek :full
@03:00:20.17 Lysis1.seek 138uL
@03:00:34.05 delay 1 second
@03:00:35.08 <fillArchive> Archive.seek :full
@03:00:35.12 <PROCESSING> Lysis1.seek :empty
@03:00:49.44 Initial air pressure @42.4psi
@03:00:49.52 Lysis1.in :extract
@03:00:49.61 Lysis1.seek 600uL,nil
@03:00:56.36 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@03:00:56.37 Archive stroke #1 of 6
@03:00:56.50 Archive.in :pressurize
@03:00:56.57 Archive.seek :pressurized
@03:01:09.30 15.5psi after pressurize
@03:01:09.31 Archive.in :compress
@03:01:09.39 Archive.seek :empty
@03:01:17.70 Archive.in :default
@03:01:17.75 Archive.hold
@03:01:17.99 <PROCESSING> Initial 600uL lysate @18.7psi
@03:01:18.02 Handoff.select :blocked
@03:01:18.12 Initial air pressure @19.5psi
@03:01:18.18 Lysis1.seek 825uL,nil
@03:01:19.95 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@03:01:19.96 Archive stroke #1 of 6
@03:01:20.11 Archive.in :compress
@03:01:20.17 Archive.seek :empty
@03:01:23.89 Archive.in :default
@03:01:23.94 Archive.hold
@03:01:24.22 <PROCESSING> Recovered 825uL lysate @17.6psi
@03:01:24.23 Air.in :halfSpeed
@03:01:24.30 Air.seek :fullVent
@03:01:26.56 Puck Top Vented, bottom @5.4psi
@03:01:26.58 Handoff.select :waste
@03:01:26.70 delay 5 seconds
@03:01:31.76 Handoff open to waste, bottom @0.2psi
@03:01:31.78 Air.in :default
@03:01:31.84 Air.seek :evac
@03:01:33.28 Lysis2.in :DAmix
@03:01:33.38 Lysis1.in :DAmix
@03:01:33.91 <Lysis2mixer> Lysis2.seek 1480uL
@03:01:33.95 <PROCESSING> Lysis1.seek 575uL
@03:02:09.00 delay 5 seconds
@03:02:14.02 Handoff.select SPR
@03:02:14.14 SPR.command \"start 2 14\"
@03:02:15.22 SPR stabilized
@03:02:15.35 delay 10 seconds
@03:02:25.37 SPR.command \"start 2 0\"
@03:02:28.58 Waiting up to 11:00 for SPR to enter state 1
@03:04:26.25 SPR.command \"hold_on\"
@03:04:27.64 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2
@03:04:28.07 <Lysis2mixer> Lysis2.seek 1180uL
@03:04:28.11 <PROCESSING> Lysis1.seek 275uL
@03:05:10.13 delay 15 seconds
@03:05:25.15 SPR.command \"hold_off\"
@03:05:28.30 Waiting up to 38:00 for SPR to enter state 7
@03:29:25.44 SPR analysis completed
@03:29:29.73 <SPRlogger> Ended log spr21aug0502h.spr.bz2
@03:29:29.74 <PROCESSING> SPR.command \"stats_off\"
@03:29:31.32 <LRAUV> -> Cmd.stop
@03:29:31.33 Archive.stop
@03:29:31.45 <closeHandoff> Handoff.select :close
@03:29:31.58 <LRAUV> Gate.power :heat,:OFF
@03:29:31.60 Gate.power :cartridge,:OFF
@03:29:31.68 Carousel.seek 59
@03:29:31.86 Plunge.seek :locked
@03:29:32.74 PinPort.in :unlocking
@03:29:32.81 PinPort.seek :clear
@03:29:33.34 <Archive> Archive.in :unlocking
@03:29:33.47 Archive.seek :clear
@03:29:33.51 <Air> Air.in :unlocking
@03:29:33.66 Air.seek :clear
@03:29:33.73 <Lysis1> Lysis1.in :unlocking
@03:29:33.86 Lysis1.seek :clear
@03:29:33.93 <Lysis2> Lysis2.in :unlocking
@03:29:34.00 Lysis2.seek :clear
@03:29:37.57 <LRAUV> Gate.power :motor,:OFF
@03:29:37.59 Gate.power :core,:OFF
@03:29:37.61 Gate.power :analytic1,:OFF
@03:29:37.63 Gate.power :analytic2,:OFF
@03:29:37.65 Gate.power :analytic3,:OFF
@03:29:37.67 Gate.power :main,:OFF
@03:29:37.70 Safely stopped and ready to power off
##-- file closed 2021-08-05T07:35:34.441Z

##-- file opened 2021-08-05T07:35:34.446Z
@03:35:34.54 -> showStatus
@03:35:36.56 -> Cmd.loadCartridge -18
@03:35:36.62 Gate.power :main,:ON,limit to 5000mA
@03:35:36.63 Gate.power :analytic1,:ON,limit to 5000mA
@03:35:36.66 Gate.power :analytic2,:ON,limit to 5000mA
@03:35:36.68 Gate.power :analytic3,:ON,limit to 5000mA
@03:35:36.82 Powering core bus
@03:35:39.10 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@03:35:39.57 <LRAUV> Archive.configure :default
@03:35:39.69 Air.configure :default
@03:35:39.80 Lysis1.configure :default
@03:35:39.90 Lysis2.configure :default
@03:35:40.02 Plunge.configure :default
@03:35:40.15 SamplePump.configure :default
@03:35:40.29 ExtraPump.configure :default
@03:35:40.40 Toroid.configure :default
@03:35:40.53 Carousel.configure :default
@03:35:40.66 Pressure.configure :default
@03:35:40.78 PinPort.configure :default
@03:35:40.84 All elf controllers running firmware version 4.741
@03:35:40.87 Gate.power :motor,:ON,limit to 5000mA
@03:35:41.20 <Archive_home> Archive.in :default
@03:35:41.25 <Air_home> Air.in :default
@03:35:41.31 <Lysis1_home> Lysis1.in :default
@03:35:41.51 Lysis1.home.jog 0
@03:35:41.54 <Air_home> Air.home.jog 0
@03:35:41.56 <Archive_home> Archive.home.jog 0
@03:35:41.78 <Toroid_home> Toroid.in :default
@03:35:41.85 <Plunge_home> Plunge.in :default
@03:35:41.98 <PinPort_home> PinPort.in :default
@03:35:42.02 <Lysis2_home> Lysis2.in :default
@03:35:42.17 <Plunge_home> Plunge.home.jog 0
@03:35:42.20 <Toroid_home> Toroid.home.jog 0
@03:35:42.23 <PinPort_home> PinPort.home.jog 0
@03:35:42.26 <Lysis2_home> Lysis2.home.jog 0
@03:35:42.56 <Lysis1_home> Lysis1.in :homing
@03:35:42.61 <Air_home> Air.in :homing
@03:35:42.72 <Archive_home> Archive.in :homing
@03:35:42.81 <Lysis1_home> Lysis1.jog 1415
@03:35:42.84 <Air_home> Air.jog 150
@03:35:42.90 <Archive_home> Archive.jog 1400
@03:35:43.05 <Toroid_home> Toroid.in :homing
@03:35:43.09 <Plunge_home> Plunge.in :homing
@03:35:43.12 <PinPort_home> PinPort.in :homing
@03:35:43.15 <Lysis2_home> Lysis2.in :homing
@03:35:43.26 <Toroid_home> Toroid.jog 624
@03:35:43.32 <Plunge_home> Plunge.jog 1567
@03:35:43.35 <PinPort_home> PinPort.jog -109
@03:35:43.45 <Lysis2_home> Lysis2.jog 1419
@03:35:43.95 <Air_home> Air.setRawPosition! 50
@03:35:44.00 Air.seek :home
@03:35:44.11 <Lysis1_home> Lysis1.setRawPosition! 50
@03:35:44.13 <Archive_home> Archive.setRawPosition! 50
@03:35:44.15 <PinPort_home> PinPort.setRawPosition! 0
@03:35:44.23 <Lysis1_home> Lysis1.seek :home
@03:35:44.30 <Archive_home> Archive.seek :home
@03:35:44.33 <PinPort_home> PinPort.seek :home
@03:35:44.52 PinPort.in :default
@03:35:44.55 <Air_home> Air.in :default
@03:35:44.72 <Plunge_home> Plunge.setRawPosition! 50
@03:35:44.74 <Lysis1_home> Lysis1.in :default
@03:35:44.77 <Archive_home> Archive.in :default
@03:35:44.88 <Plunge_home> Plunge.seek :home
@03:35:45.04 <Lysis2_home> Lysis2.setRawPosition! 50
@03:35:45.09 Lysis2.seek :home
@03:35:45.20 <Plunge_home> Plunge.in :default
@03:35:45.37 <Lysis2_home> Lysis2.in :default
@03:35:45.51 <Toroid_home> Toroid.setRawPosition! 0
@03:35:45.56 Toroid.seek :home
@03:35:45.65 Toroid.in :default
@03:35:45.78 <LRAUV> Toroid.seek :clear
@03:35:47.40 Carousel.in :default
@03:35:47.46 Carousel.home.jog 0
@03:35:47.96 Carousel.in :default
@03:35:48.02 Carousel.seek 59
@03:35:48.18 Selecting Cartridge 58
@03:35:48.43 Carousel.seek 58
@03:35:48.92 Lysis1.in :fast
@03:35:48.98 Lysis1.seek :locked
@03:35:51.58 <Plunge> Plunge.seek :locked
@03:35:51.72 <Air> Air.in :fast
@03:35:51.78 Air.seek :locked
@03:35:54.38 <LRAUV> Powering cartridge bus
@03:35:57.00 Gate.setIR 1750,5000,0,6
@03:35:57.13 Gate.setIR 0,5000,0,6
@03:35:57.19 PuckHeater.configure :default
@03:35:57.24 Gate.power :heat,:ON,limit to 5000mA
@03:35:57.93 Mounted lysis Cartridge board#Q05037
@03:35:57.96 Using default puck heat loss curve for HeatingAccelInCarousel
@03:36:16.98 -> Cmd.startFiltering
@03:36:17.43 <FILTERING> Priming sample loop w/100ml, bypass w/10ml
@03:36:17.45 SamplePump.setPosition! 0ml
@03:36:17.49 Bypass.open
@03:36:18.95 Intake.open
@03:36:19.73 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@03:36:22.30 Vacuum tare is 50% of full scale
@03:36:22.43 Pressure.in :default
@03:36:22.48 Pressure.hold
@03:36:22.65 SamplePump.in :prime
@03:36:22.72 SamplePump.seek 10ml,59 seconds
@03:36:31.97 Pressure.stop
@03:36:32.02 SamplePump.setPosition! 0ml
@03:36:32.07 Exhaust.open
@03:36:33.51 <closeBypass> Bypass.close
@03:36:35.05 <FILTERING> Pressure.in :default
@03:36:35.11 Pressure.hold
@03:36:35.33 SamplePump.in :prime
@03:36:35.40 SamplePump.seek 100ml,5:16
@03:38:30.68 Pressure.stop
@03:38:30.74 Bypass.open
@03:38:31.46 Intake.close
@03:38:32.27 Toroid.in :hold
@03:38:32.33 Toroid.seek :crack
@03:38:33.84 Toroid.seek :cartridge
@03:38:34.93 Toroid.in :default
@03:38:34.99 Toroid.seek :clear
@03:38:36.95 Pressurizing cartridge to 14psi above 13.3psia
@03:38:36.96 SamplePump.setPosition! 0ml
@03:38:37.00 Intake.open
@03:38:37.74 Bypass.close
@03:38:38.43 Exhaust.close
@03:38:39.22 Pressure.in :default
@03:38:39.28 Pressure.hold
@03:38:39.45 SamplePump.in :charge
@03:38:39.51 SamplePump.seek 2.3ml,41 seconds
@03:38:52.14 Pressure.stop
@03:38:52.19 Intake.close
@03:38:53.02 Pressurized cartridge with 0.713ml to 14.5psi
@03:38:53.03 Isolated @24.2psia (10.9psi)
@03:38:53.04 Verifying cartridge holds at least 0.75..0.85 of 24.2psia
@03:38:53.07 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@03:38:57.64 Cartridge pressure fell 6% to 22.8psia
@03:38:57.72 Sampling 75.0ml
@03:38:57.73 SamplePump.setPosition! 0ml
@03:38:57.78 Exhaust.open
@03:38:58.45 Intake.open
@03:38:59.31 Pressure.in :default
@03:38:59.37 Pressure.hold
@03:38:59.59 SamplePump.in :sample
@03:38:59.66 SamplePump.seek 75ml,5:31
@03:39:02.34 <sampling> Cartridge 58 filtering 1/75ml at 0.39ml/s with 15.1psi vacuum
@03:40:02.33 Cartridge 58 filtering 23/75ml at 0.37ml/s with 17.5psi vacuum
@03:41:02.34 Cartridge 58 filtering 45/75ml at 0.36ml/s with 12.0psi vacuum
@03:42:02.33 Cartridge 58 filtering 67/75ml at 0.39ml/s with 11.3psi vacuum
@03:42:23.81 <FILTERING> Pressure.stop
@03:42:23.93 <closeIntake> Intake.close
@03:42:23.97 <closeExhaust> Exhaust.close
@03:42:24.12 <FILTERING> Toroid.in :hold
@03:42:24.19 Toroid.seek :bypass
@03:42:25.80 Toroid.in :default
@03:42:25.86 Toroid.seek :clear
@03:42:27.47 Sampled  75.0ml
@03:42:28.74 <LRAUV> -> Cmd.startProcessing
@03:42:30.26 <PROCESSING> SPR.command \"read_sequences\"
@03:42:30.76 SPR.command \"stats_on\"
@03:42:31.37 SPR.command \"start 0 1\"
@03:42:32.17 <SPRlogger> SPR.start /var/log/esp/spr21aug0503h.spr.bz2
@03:42:34.21 <PROCESSING> Handoff.select :waste
@03:42:40.90 Syringe tare is 19% of full scale
@03:42:40.93 PinPort.in :locking
@03:42:41.01 PinPort.seek :locked
@03:42:41.49 <posArchive> Archive.in :fast
@03:42:41.55 <posLyS2> Lysis2.in :fast
@03:42:41.60 <PROCESSING> Lysis1.seek 1350uL
@03:42:41.69 <posArchive> Archive.seek :full
@03:42:41.75 <posLyS2> Lysis2.seek :full
@03:42:43.88 <PROCESSING> Plunge.seek :reagent
@03:42:44.78 Evacuating puck with filter bubble point of 24psi
@03:42:44.80 PinPort.seek :locked
@03:42:44.88 Handoff.select :waste
@03:42:51.47 Air syringe pumping up to 1 stroke to reach 8psi
@03:42:51.48 Air stroke #1 of 1
@03:42:51.57 Air.in :pressurize
@03:42:51.63 Air.seek :pressurized
@03:43:02.88 0.1psi after pressurize
@03:43:02.89 Air.in :compress
@03:43:02.96 Air.seek :evac
@03:43:07.05 0.6psi after compress
@03:43:19.62 0.3psi after compress (stable)
@03:43:19.63 Air.in :default
@03:43:19.71 Air.hold
@03:43:19.88 at 0.4psi Opened to waste
@03:43:19.89 Handoff.select :blocked
@03:43:20.02 Archive syringe pumping up to 8 strokes to reach 24psi
@03:43:20.03 Archive stroke #1 of 8
@03:43:20.12 Archive.in :pressurize
@03:43:20.20 Archive.seek :pressurized
@03:43:32.94 3.3psi after pressurize
@03:43:32.95 Archive.in :compress
@03:43:33.03 Archive.seek :empty
@03:43:48.90 14.0psi after compress
@03:44:01.47 14.5psi after compress (stable)
@03:44:01.49 Archive.in :gulp
@03:44:01.56 Archive.seek :topVent
@03:44:08.76 14.7psi after gulp
@03:44:21.33 14.3psi after gulp (stable)
@03:44:21.34 Archive stroke #2 of 8
@03:44:21.43 Archive.in :pressurize
@03:44:21.49 Archive.seek :pressurized
@03:44:34.21 14.5psi after pressurize
@03:44:34.22 Archive.in :compress
@03:44:34.30 Archive.seek :empty
@03:44:48.55 Reached 24.0 of 24.0psi after pumping 3426uL (1.98 strokes)
@03:44:48.56 Archive.in :default
@03:44:48.63 Archive.hold
@03:44:48.72 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position
@03:44:48.74 Handoff.select :waste
@03:44:59.46 delay 1:00
@03:45:59.49 Handoff.select :blocked
@03:46:22.17 Handoff.select :waste
@03:46:22.29 0.1psi at evacEnd
@03:46:22.30 Handoff.select :blocked
@03:46:22.44 Archive.in :fast
@03:46:22.50 Archive.seek :topVent
@03:46:36.08 Stable @0.1psi after Handoff blocked
@03:46:36.10 Archive syringe pumping up to 8 strokes to reach 15psi
@03:46:36.11 Archive stroke #1 of 8
@03:46:36.21 Archive.in :pressurize
@03:46:36.27 Archive.seek :pressurized
@03:46:48.99 1.5psi after pressurize
@03:46:49.00 Archive.in :compress
@03:46:49.08 Archive.seek :empty
@03:47:04.96 12.7psi after compress
@03:47:17.55 13.0psi after compress (stable)
@03:47:17.59 Archive.in :gulp
@03:47:17.66 Archive.seek :topVent
@03:47:24.86 13.1psi after gulp
@03:47:37.43 12.7psi after gulp (stable)
@03:47:37.44 Archive stroke #2 of 8
@03:47:37.53 Archive.in :pressurize
@03:47:37.59 Archive.seek :pressurized
@03:47:50.31 13.0psi after pressurize
@03:47:50.32 Archive.in :compress
@03:47:50.40 Archive.seek :empty
@03:47:54.07 Reached 15.2 of 15.1psi after pumping 3199uL (1.85 strokes)
@03:47:54.08 Archive.in :default
@03:47:54.15 Archive.hold
@03:47:54.24 Slide::Error in PROCESSING -- Slide::Error while holding Archive Syringe (ArS) in position
@03:47:54.71 Handoff.select :waste
@03:48:01.91 delay 1:00
@03:49:01.93 Handoff.select :blocked
@03:49:24.60 Handoff.select :waste
@03:49:24.72 0.1psi at evacEnd
@03:49:24.74 Air.seek :fullVent
@03:49:26.18 Archive.in :fast
@03:49:26.24 Archive.seek :topVent
@03:49:32.46 Seawater evacuated, Presure above/below filter released. Lysis begins
@03:49:32.55 Lysis1.in :slow
@03:49:32.61 Lysis1.seek 1050uL
@03:50:02.61 delay 5 seconds
@03:50:07.63 Handoff.select :blocked
@03:50:07.82 Lysis1.seek 450uL
@03:51:07.71 delay 3 seconds
@03:51:10.78 Air.in to mix into 1.37806 parts Lysis1
@03:51:11.35 <Airmixer> Air.seek :evac
@03:51:11.40 <PROCESSING> Lysis1.seek 300uL
@03:51:26.48 Lysis1.seek :empty
@03:51:56.59 Lysis buffer delivered @7.2psi
@03:51:56.60 Polling Can every 2:00
@03:51:57.15 PuckHeater.configure :default #ambient 23.8C
@03:51:57.19 PuckHeater.seek 79.7C
@03:52:39.38 PuckHeater.hold 2:00,79.7C
@03:54:39.00 Polling Can every 2:00
@03:54:39.55 PuckHeater.configure :default #ambient 23.8C
@03:54:39.59 PuckHeater.hold 30 seconds,78.6C
@03:55:09.67 Polling Can every 2:00
@03:55:10.22 PuckHeater.hold 30 seconds,78.1C
@03:55:40.29 Polling Can every 2:00
@03:55:40.83 PuckHeater.configure :default #ambient 23.8C
@03:55:40.88 PuckHeater.hold 10:00,77.6C
@04:05:38.62 Lysis complete. Syringe pressure @6.6psi
@04:05:38.64 Handoff.select :blocked
@04:05:38.69 Archive syringe pumping up to 5 strokes to reach 40psi
@04:05:38.70 Archive stroke #1 of 5
@04:05:38.78 Archive.in :pressurize
@04:05:38.85 Archive.seek :pressurized
@04:05:51.57 11.7psi after pressurize
@04:05:51.59 Archive.in :compress
@04:05:51.66 Archive.seek :empty
@04:06:04.54 Reached 40.2 of 40.0psi after pumping 1666uL (0.96 strokes)
@04:06:04.55 Archive.in :default
@04:06:04.63 Archive.hold
@04:06:04.69 delay 2 seconds
@04:06:06.71 Archive.in :fast
@04:06:06.78 Archive.seek :full
@04:06:13.77 Lysis1.seek 138uL
@04:06:27.63 delay 1 second
@04:06:28.67 <fillArchive> Archive.seek :full
@04:06:28.69 <PROCESSING> Lysis1.seek :empty
@04:06:42.67 Initial air pressure @39.4psi
@04:06:42.74 Lysis1.in :extract
@04:06:42.83 Lysis1.seek 600uL,nil
@04:06:51.47 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@04:06:51.48 Archive stroke #1 of 6
@04:06:51.57 Archive.in :pressurize
@04:06:51.64 Archive.seek :pressurized
@04:07:04.43 15.1psi after pressurize
@04:07:04.45 Archive.in :compress
@04:07:04.53 Archive.seek :empty
@04:07:06.31 Archive.in :default
@04:07:06.36 Archive.hold
@04:07:06.60 <PROCESSING> Initial 600uL lysate @17.6psi
@04:07:06.62 Handoff.select :blocked
@04:07:06.71 Initial air pressure @17.8psi
@04:07:06.78 Lysis1.seek 825uL,nil
@04:07:09.65 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@04:07:09.67 Archive stroke #1 of 6
@04:07:09.76 Archive.in :compress
@04:07:09.82 Archive.seek :empty
@04:07:11.53 Archive.in :default
@04:07:11.58 Archive.hold
@04:07:11.87 <PROCESSING> Recovered 825uL lysate @15.7psi
@04:07:11.88 Air.in :halfSpeed
@04:07:11.96 Air.seek :fullVent
@04:07:14.21 Puck Top Vented, bottom @11.9psi
@04:07:14.22 Handoff.select :waste
@04:07:14.34 delay 5 seconds
@04:07:19.41 Handoff open to waste, bottom @0.1psi
@04:07:19.43 Air.in :default
@04:07:19.50 Air.seek :evac
@04:07:20.94 Lysis2.in :DAmix
@04:07:21.05 Lysis1.in :DAmix
@04:07:21.59 <Lysis2mixer> Lysis2.seek 1480uL
@04:07:21.65 <PROCESSING> Lysis1.seek 575uL
@04:07:56.70 delay 5 seconds
@04:08:01.72 Handoff.select SPR
@04:08:01.85 SPR.command \"start 2 14\"
@04:08:03.22 SPR stabilized
@04:08:03.34 delay 10 seconds
@04:08:13.36 SPR.command \"start 2 0\"
@04:08:16.07 Waiting up to 11:00 for SPR to enter state 1
@04:10:13.74 SPR.command \"hold_on\"
@04:10:15.64 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2
@04:10:16.09 <Lysis2mixer> Lysis2.seek 1180uL
@04:10:16.13 <PROCESSING> Lysis1.seek 275uL
@04:10:58.13 delay 15 seconds
@04:11:13.15 SPR.command \"hold_off\"
@04:11:15.78 Waiting up to 38:00 for SPR to enter state 7
@04:35:11.98 SPR analysis completed
@04:35:16.28 <SPRlogger> Ended log spr21aug0503h.spr.bz2
@04:35:16.29 <PROCESSING> SPR.command \"stats_off\"
@04:35:17.73 <LRAUV> -> Cmd.stop
@04:35:17.74 Archive.stop
@04:35:17.86 <closeHandoff> Handoff.select :close
@04:35:17.99 <LRAUV> Gate.power :heat,:OFF
@04:35:18.01 Gate.power :cartridge,:OFF
@04:35:18.09 Carousel.seek 58
@04:35:18.27 Plunge.seek :locked
@04:35:19.15 PinPort.in :unlocking
@04:35:19.21 PinPort.seek :clear
@04:35:19.75 <Archive> Archive.in :unlocking
@04:35:19.88 Archive.seek :clear
@04:35:19.92 <Air> Air.in :unlocking
@04:35:20.07 Air.seek :clear
@04:35:20.16 <Lysis1> Lysis1.in :unlocking
@04:35:20.27 Lysis1.seek :clear
@04:35:20.33 <Lysis2> Lysis2.in :unlocking
@04:35:20.41 Lysis2.seek :clear
@04:35:23.99 <LRAUV> Gate.power :motor,:OFF
@04:35:24.01 Gate.power :core,:OFF
@04:35:24.03 Gate.power :analytic1,:OFF
@04:35:24.05 Gate.power :analytic2,:OFF
@04:35:24.08 Gate.power :analytic3,:OFF
@04:35:24.10 Gate.power :main,:OFF
@04:35:24.12 Safely stopped and ready to power off
##-- file closed 2021-08-05T20:24:52.812Z