##-- file opened 2020-06-10T21:16:03.092Z @14:16:03.35 -> showStatus @14:16:04.98 -> Cmd.loadCartridge -6 @14:16:05.18 Gate.power :main,:ON,limit to 5000mA @14:16:05.22 Gate.power :analytic1,:ON,limit to 5000mA @14:16:05.25 Gate.power :analytic2,:ON,limit to 5000mA @14:16:05.28 Gate.power :analytic3,:ON,limit to 5000mA @14:16:05.37 Powering core bus @14:16:07.66 Archive.configure :default @14:16:07.83 Air.configure :default @14:16:07.96 Lysis1.configure :default @14:16:08.09 Lysis2.configure :default @14:16:08.24 Plunge.configure :default @14:16:08.40 SamplePump.configure :default @14:16:08.55 ExtraPump.configure :default @14:16:08.71 Toroid.configure :default @14:16:08.87 Carousel.configure :default @14:16:09.03 Pressure.configure :default @14:16:09.11 All elf controllers running firmware version 4.58 @14:16:09.15 Gate.power :motor,:ON,limit to 5000mA @14:16:09.36 Archive.reconfigure :default @14:16:10.14 Archive.home.jog 0 @14:16:10.57 Plunge.reconfigure :default @14:16:10.68 Lysis2.reconfigure :default @14:16:10.77 Air.reconfigure :default @14:16:10.88 Lysis1.reconfigure :default @14:16:10.98 Toroid.reconfigure :default @14:16:11.23 Plunge.home.jog 0 @14:16:11.40 Air.home.jog 0 @14:16:11.44 Lysis2.home.jog 0 @14:16:11.55 Lysis1.home.jog 0 @14:16:11.58 Toroid.home.jog 0 @14:16:12.34 Archive.reconfigure :homing @14:16:12.60 Archive.jog 1349 @14:16:12.66 Toroid.reconfigure :homing @14:16:12.71 Plunge.reconfigure :homing @14:16:12.75 Lysis1.reconfigure :homing @14:16:12.80 Lysis2.reconfigure :homing @14:16:12.85 Air.reconfigure :homing @14:16:13.13 Toroid.jog 613 @14:16:13.18 Plunge.jog 108 @14:16:13.22 Lysis1.jog 1358 @14:16:13.27 Lysis2.jog 1353 @14:16:13.36 Air.jog 1352 @14:16:13.76 Plunge.setRawPosition! 50 @14:16:13.83 Plunge.seek :home @14:16:14.04 Archive.setRawPosition! 50 @14:16:14.12 Archive.seek :home @14:16:14.22 Plunge.reconfigure :default @14:16:14.42 Archive.reconfigure :default @14:16:14.59 Lysis1.setRawPosition! 50 @14:16:14.67 Lysis1.seek :home @14:16:14.78 Lysis2.setRawPosition! 50 @14:16:14.81 Air.setRawPosition! 50 @14:16:14.91 Lysis2.seek :home @14:16:14.96 Air.seek :home @14:16:15.27 Lysis1.reconfigure :default @14:16:15.40 Lysis2.reconfigure :default @14:16:15.44 Air.reconfigure :default @14:16:15.48 Toroid.setRawPosition! 0 @14:16:15.67 Toroid.seek :home @14:16:15.79 Toroid.reconfigure :default @14:16:16.00 Toroid.seek :clear @14:16:17.67 Carousel.reconfigure :default @14:16:17.80 Carousel.home.jog 0 @14:16:18.44 Carousel.seek 1 @14:16:18.70 Selecting Cartridge 60 @14:16:19.24 Carousel.seek 60 @14:16:31.76 Lysis1.seek :locked @14:16:35.06 Plunge.seek :locked @14:16:35.24 Air.seek :locked @14:16:40.52 -> Cmd.startFiltering @14:16:40.83 Duration of filtering limited to 2:05:00 @14:16:41.17 Priming sample loop w/100ml, bypass w/10ml @14:16:41.19 SamplePump.setPosition! 0ml @14:16:41.27 Bypass.open @14:16:42.77 Intake.open @14:16:43.65 Sea 14.0m deep (35.3psia) @14:16:43.68 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:16:47.10 Vacuum tare is 45% of full scale @14:16:47.26 Pressure.reconfigure :default @14:16:47.33 Pressure.hold @14:16:47.57 SamplePump.reconfigure :prime @14:16:47.65 SamplePump.seek 10ml,1:21 @14:16:59.02 Pressure.coast @14:16:59.27 SamplePump.setPosition! 0ml @14:16:59.39 Exhaust.open @14:17:00.98 Bypass.close @14:17:02.63 Pressure.reconfigure :default @14:17:02.70 Pressure.hold @14:17:02.86 Sea 13.8m deep (35.0psia) @14:17:03.04 SamplePump.reconfigure :prime @14:17:03.12 SamplePump.seek 100ml,8:51 @14:18:51.30 Pressure.coast @14:18:51.47 Bypass.open @14:18:52.19 Intake.close @14:18:53.75 Toroid.reconfigure :hold @14:18:53.84 Toroid.seek :crack @14:18:54.78 Toroid.reconfigure :default @14:18:54.85 Toroid.seek :clear @14:18:56.60 Toroid.reconfigure :hold @14:18:56.67 Toroid.seek :cartridge @14:18:58.50 Toroid.reconfigure :default @14:18:58.63 Toroid.seek :clear @14:19:00.72 Pressurizing cartridge to 26psi above 34.8psia @14:19:00.73 SamplePump.setPosition! 0ml @14:19:00.81 Intake.open @14:19:01.58 Bypass.close @14:19:02.21 Exhaust.close @14:19:03.12 Pressure.reconfigure :default @14:19:03.20 Pressure.hold @14:19:03.44 SamplePump.reconfigure :charge @14:19:03.52 SamplePump.seek 2.3ml,42 seconds @14:19:10.98 Pressure.coast @14:19:11.05 Intake.close @14:19:11.87 Pressurized cartridge with 0.248ml to 26.2psi @14:19:11.88 Isolated @57.8psia (23.0psi) @14:19:11.91 Checking for cartridge leaks from 57.8psia @14:19:12.35 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @14:19:17.80 Cartridge pressure fell 3% to 56.3psia @14:19:17.90 Sampling 1000.0ml @14:19:17.92 SamplePump.setPosition! 0ml @14:19:18.00 Exhaust.open @14:19:18.80 Intake.open @14:19:19.88 Pressure.reconfigure :default @14:19:20.04 Pressure.hold @14:19:20.28 Sea 13.4m deep (34.5psia) @14:19:20.57 SamplePump.reconfigure :sample @14:19:20.69 SamplePump.seek 1000ml,1:23:53 @14:19:22.86 Cartridge 60 sampling 1/1000ml at 0.37ml/s with 21.3psi vacuum 12.3m deep (32.8psia) @14:20:22.95 Cartridge 60 sampling 24/1000ml at 0.41ml/s with 24.1psi vacuum 13.2m deep (34.3psia) @14:21:22.86 Cartridge 60 sampling 47/1000ml at 0.40ml/s with 24.0psi vacuum 12.3m deep (32.9psia) @14:22:22.88 Cartridge 60 sampling 69/1000ml at 0.35ml/s with 21.9psi vacuum 12.7m deep (33.4psia) @14:23:22.86 Cartridge 60 sampling 91/1000ml at 0.36ml/s with 22.1psi vacuum 9.7m deep (29.1psia) @14:24:22.86 Cartridge 60 sampling 113/1000ml at 0.39ml/s with 23.3psi vacuum 13.8m deep (35.1psia) @14:25:22.86 Cartridge 60 sampling 134/1000ml at 0.38ml/s with 24.7psi vacuum 12.4m deep (33.0psia) @14:26:22.86 Cartridge 60 sampling 155/1000ml at 0.35ml/s with 22.0psi vacuum 11.9m deep (32.2psia) @14:27:23.11 Cartridge 60 sampling 175/1000ml at 0.33ml/s with 27.5psi vacuum 10.9m deep (30.7psia) @14:28:22.86 Cartridge 60 sampling 196/1000ml at 0.34ml/s with 26.7psi vacuum 12.2m deep (32.8psia) @14:29:23.11 Cartridge 60 sampling 215/1000ml at 0.36ml/s with 25.4psi vacuum 12.7m deep (33.5psia) @14:30:22.86 Cartridge 60 sampling 235/1000ml at 0.32ml/s with 21.7psi vacuum 12.7m deep (33.4psia) @14:31:23.12 Cartridge 60 sampling 254/1000ml at 0.28ml/s with 25.5psi vacuum 11.8m deep (32.1psia) @14:32:22.86 Cartridge 60 sampling 272/1000ml at 0.35ml/s with 23.0psi vacuum 13.6m deep (34.8psia) @14:33:22.86 Cartridge 60 sampling 291/1000ml at 0.30ml/s with 26.2psi vacuum 13.2m deep (34.3psia) @14:34:22.86 Cartridge 60 sampling 309/1000ml at 0.26ml/s with 23.7psi vacuum 9.0m deep (27.9psia) @14:35:22.86 Cartridge 60 sampling 326/1000ml at 0.25ml/s with 20.8psi vacuum 11.1m deep (31.0psia) @14:36:23.00 Cartridge 60 sampling 343/1000ml at 0.25ml/s with 23.1psi vacuum 10.8m deep (30.7psia) @14:37:22.86 Cartridge 60 sampling 360/1000ml at 0.26ml/s with 26.0psi vacuum 11.5m deep (31.7psia) @14:38:22.90 Cartridge 60 sampling 377/1000ml at 0.31ml/s with 21.0psi vacuum 14.7m deep (36.4psia) @14:39:22.86 Cartridge 60 sampling 393/1000ml at 0.28ml/s with 22.1psi vacuum 12.6m deep (33.3psia) @14:40:22.86 Cartridge 60 sampling 409/1000ml at 0.24ml/s with 23.8psi vacuum 11.7m deep (32.0psia) @14:41:22.85 Cartridge 60 sampling 425/1000ml at 0.27ml/s with 26.3psi vacuum 12.7m deep (33.4psia) @14:42:22.86 Cartridge 60 sampling 440/1000ml at 0.29ml/s with 25.8psi vacuum 12.2m deep (32.8psia) @14:43:22.98 Cartridge 60 sampling 455/1000ml at 0.26ml/s with 22.4psi vacuum 12.5m deep (33.2psia) @14:44:22.86 Cartridge 60 sampling 470/1000ml at 0.22ml/s with 24.2psi vacuum 12.2m deep (32.7psia) @14:45:22.95 Cartridge 60 sampling 484/1000ml at 0.21ml/s with 24.9psi vacuum 11.8m deep (32.2psia) @14:46:22.86 Cartridge 60 sampling 499/1000ml at 0.21ml/s with 25.9psi vacuum 11.0m deep (30.9psia) @14:47:22.97 Cartridge 60 sampling 513/1000ml at 0.21ml/s with 24.4psi vacuum 12.3m deep (32.8psia) @14:48:22.86 Cartridge 60 sampling 526/1000ml at 0.21ml/s with 18.1psi vacuum 13.7m deep (34.9psia) @14:49:22.86 Cartridge 60 sampling 540/1000ml at 0.21ml/s with 24.2psi vacuum 13.0m deep (33.9psia) @14:50:23.16 Cartridge 60 sampling 553/1000ml at 0.20ml/s with 24.8psi vacuum 9.5m deep (28.7psia) @14:51:22.86 Cartridge 60 sampling 566/1000ml at 0.25ml/s with 23.6psi vacuum 13.2m deep (34.3psia) @14:52:22.95 Cartridge 60 sampling 579/1000ml at 0.22ml/s with 26.6psi vacuum 11.4m deep (31.6psia) @14:53:22.86 Cartridge 60 sampling 592/1000ml at 0.22ml/s with 24.5psi vacuum 12.3m deep (32.9psia) @14:54:22.90 Cartridge 60 sampling 605/1000ml at 0.22ml/s with 25.2psi vacuum 11.0m deep (30.9psia) @14:55:22.86 Cartridge 60 sampling 617/1000ml at 0.20ml/s with 28.1psi vacuum 11.0m deep (30.9psia) @14:56:22.86 Cartridge 60 sampling 630/1000ml at 0.21ml/s with 24.8psi vacuum 12.3m deep (32.9psia) @14:57:22.86 Cartridge 60 sampling 642/1000ml at 0.21ml/s with 23.6psi vacuum 12.9m deep (33.8psia) @14:58:22.86 Cartridge 60 sampling 654/1000ml at 0.20ml/s with 28.1psi vacuum 12.3m deep (32.8psia) @14:59:22.96 Cartridge 60 sampling 666/1000ml at 0.21ml/s with 22.7psi vacuum 13.8m deep (35.1psia) @15:00:22.86 Cartridge 60 sampling 679/1000ml at 0.20ml/s with 29.8psi vacuum 11.9m deep (32.3psia) @15:01:17.73 Flow averaged 0.27ml/s over 41:54 @15:01:17.78 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @15:01:18.53 Relieved excess vacuum in 4.6 seconds @15:01:18.57 SamplePump.seek 1000ml,26:22 @15:01:22.96 Cartridge 60 sampling 691/1000ml at 0.20ml/s with 26.7psi vacuum 12.5m deep (33.1psia) @15:02:22.86 Cartridge 60 sampling 703/1000ml at 0.20ml/s with 25.8psi vacuum 12.5m deep (33.2psia) @15:03:23.05 Cartridge 60 sampling 715/1000ml at 0.20ml/s with 26.8psi vacuum 9.8m deep (29.2psia) @15:04:22.86 Cartridge 60 sampling 727/1000ml at 0.20ml/s with 27.4psi vacuum 12.0m deep (32.4psia) @15:05:22.86 Cartridge 60 sampling 739/1000ml at 0.20ml/s with 26.4psi vacuum 12.1m deep (32.6psia) @15:05:28.71 Flow averaged 0.21ml/s over 4:05 @15:05:29.02 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @15:05:29.78 Relieved excess vacuum in 6.8 seconds @15:05:29.82 SamplePump.seek 1000ml,22:08 @15:06:17.15 Flow averaged 0.22ml/s over 45 seconds @15:06:17.29 Waiting up to 14 seconds for vacuum to reduce from 28.5 to 18.0psi @15:06:18.13 Relieved excess vacuum in 3.8 seconds @15:06:18.17 SamplePump.seek 1000ml,21:20 @15:06:23.31 Cartridge 60 sampling 751/1000ml at 0.20ml/s with 30.3psi vacuum 9.3m deep (28.4psia) @15:06:48.88 Flow averaged 0.24ml/s over 26 seconds @15:06:48.93 Waiting up to 12 seconds for vacuum to reduce from 27.7 to 18.0psi @15:06:49.69 Relieved excess vacuum in 6.2 seconds @15:06:49.73 SamplePump.seek 1000ml,20:49 @15:06:53.21 Flow estimate reduced to 0.20ml/s @15:06:53.24 Intake.close @15:06:53.28 Exhaust.close @15:06:53.55 Toroid.reconfigure :hold @15:06:53.62 Toroid.seek :bypass @15:06:55.26 Toroid.reconfigure :default @15:06:55.34 Toroid.seek :clear @15:06:56.98 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @15:06:57.00 Pressure.coast @15:06:57.26 Sampled 757.0ml @15:06:59.43 -> Cmd.startProcessing @15:07:00.09 Plunge.seek :reagent @15:07:01.08 Sample loop closed. Ready to Deliver Reagents @15:07:01.10 Archive.seek :sealed @15:07:04.79 Archive.seek :evac @15:07:09.60 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 1143ul (sensed 1174ul) @15:07:09.63 Retry #1 of 2 @15:07:09.65 Archive.hold @15:07:10.07 Archive.reconfigure :default @15:07:10.15 Archive.home.seek :evac @15:07:12.31 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 960ul (sensed 985ul) @15:07:12.38 Retry #2 of 2 @15:07:12.41 Archive.reconfigure :default @15:07:12.55 Archive.hold @15:07:12.74 Archive.reconfigure :default @15:07:12.88 Archive.home.seek :evac @15:07:15.28 Slide::Error in PROCESSING -- IGNORED Archive Syringe (ArS) positionErr at 741ul (sensed 765ul) @15:07:15.30 Archive.reconfigure :default @15:07:15.38 Archive.hold @15:07:15.69 Archive.reconfigure :default @15:07:15.76 Archive.home.jog 0 @15:07:15.91 ArchiveError in PROCESSING -- 740ul RNAlater undelivered @15:07:15.96 Thread::ChildDied in LRAUV -- :PROCESSING @15:07:20.43 -> Cmd.stop @15:07:20.73 Carousel.seek 60 @15:07:21.06 Plunge.seek :locked @15:07:22.30 Archive.seek :clear @15:07:22.48 Air.seek :clear @15:07:22.67 Lysis1.seek :clear @15:07:32.89 Archive.coast @15:07:32.96 Air.coast @15:07:33.03 Lysis1.coast @15:07:33.11 Lysis2.coast @15:07:33.57 Plunge.coast @15:07:33.73 SamplePump.coast @15:07:33.84 Toroid.coast @15:07:33.96 Carousel.coast @15:07:34.29 Gate.power :motor,:OFF @15:07:34.32 Gate.power :core,:OFF @15:07:34.39 Gate.power :analytic1,:OFF @15:07:34.43 Gate.power :analytic2,:OFF @15:07:34.50 Gate.power :analytic3,:OFF @15:07:34.53 Gate.power :main,:OFF @15:07:34.60 Safely stopped and ready to power off ##-- file closed 2020-06-11T16:17:43.906Z