##-- file opened 2025-12-05T18:05:52.764Z
@10:05:53.18 -> showStatus
@10:05:54.80 -> Cmd.loadCartridge
@10:05:54.89 Gate.power :main,:ON,limit to 5000mA
@10:05:54.91 Gate.power :analytic1,:ON,limit to 4000mA
@10:05:54.94 Gate.power :analytic2,:ON,limit to 4000mA
@10:05:54.96 Gate.power :analytic3,:ON,limit to 4000mA
@10:05:55.01 Powering core bus
@10:05:57.72 Plunge.configure :default
@10:05:57.80 SamplePump.configure :default
@10:05:57.88 Toroid.configure :default
@10:05:57.96 Carousel.configure :default
@10:05:58.03 Abs.configure :default
@10:05:58.11 Archive.configure :default
@10:05:58.19 Air.configure :default
@10:05:58.25 Lysis1.configure :default
@10:05:58.30 Lysis2.configure :default
@10:05:58.39 PinPort.configure :default
@10:05:58.46 WARNING:  Multiple controller firmware versions in use
[:sampler0, :syringe1, :sampler1, :valve0, :ambient0, :valve1, :syringe0]=>4.93, [:carousel0]=>4.94
@10:05:58.48 Gate.power :motor,:ON,limit to 5000mA
@10:05:58.71 <Plunge_home> Plunge.home.jog 0
@10:05:59.20 <PinPort_home> PinPort.home.jog 0
@10:05:59.24 <Lysis2_home> Lysis2.home.jog 0
@10:05:59.26 <Lysis1_home> Lysis1.home.jog 0
@10:05:59.29 <Air_home> Air.home.jog 0
@10:05:59.32 <Archive_home> Archive.home.jog 0
@10:05:59.35 <Carousel_home> Carousel.home.jog 0
@10:05:59.38 <Toroid_home> Toroid.home.jog 0
@10:05:59.92 <Plunge_home> Plunge.in :homing
@10:06:00.11 Plunge.jog 1525
@10:06:00.30 <Toroid_home> Toroid.in :homing
@10:06:00.33 <PinPort_home> PinPort.in :homing
@10:06:00.36 <Lysis2_home> Lysis2.in :homing
@10:06:00.40 <Lysis1_home> Lysis1.in :homing
@10:06:00.43 <Archive_home> Archive.in :homing
@10:06:00.46 <Air_home> Air.in :homing
@10:06:00.64 <PinPort_home> PinPort.jog -1203
@10:06:00.69 <Toroid_home> Toroid.jog 1301
@10:06:00.72 <Lysis2_home> Lysis2.jog 1612
@10:06:00.74 <Lysis1_home> Lysis1.jog 304
@10:06:00.78 <Archive_home> Archive.jog 322
@10:06:00.80 <Air_home> Air.jog 359
@10:06:01.40 <Plunge_home> Plunge.setRawPosition! 50
@10:06:01.45 Plunge.seek :home
@10:06:01.60 <Air_home> Air.setRawPosition! 0
@10:06:01.64 <Archive_home> Archive.setRawPosition! 50
@10:06:01.66 <Lysis1_home> Lysis1.setRawPosition! 50
@10:06:01.81 <Air_home> Air.seek :home
@10:06:01.83 <Archive_home> Archive.seek :home
@10:06:01.87 <Lysis1_home> Lysis1.seek :home
@10:06:02.08 <Air_home> Air.in :default
@10:06:02.10 <Plunge_home> Plunge.in :default
@10:06:02.24 <Lysis2_home> Lysis2.setRawPosition! 50
@10:06:02.27 <Archive_home> Archive.in :default
@10:06:02.34 <Lysis2_home> Lysis2.seek :home
@10:06:02.45 <Lysis1_home> Lysis1.in :default
@10:06:02.55 <PinPort_home> PinPort.setRawPosition! 0
@10:06:02.60 PinPort.seek :home
@10:06:02.72 <Lysis2_home> Lysis2.in :default
@10:06:02.74 <PinPort_home> PinPort.in :default
@10:06:05.19 <Toroid_home> Toroid.setRawPosition! 0
@10:06:05.25 Toroid.seek :home
@10:06:05.32 Toroid.in :default
@10:06:05.39 <clearToroid> Toroid.seek :clear
@10:06:05.89 <LRAUV> PinPort.in :unlocking
@10:06:05.96 PinPort.seek :clear
@10:06:09.35 Air.seek :clear
@10:06:10.25 Lysis1.seek :clear
@10:06:11.04 Lysis2.seek :clear
@10:06:11.83 Archive.seek :clear
@10:06:12.62 Carousel.in :default
@10:06:12.73 Carousel.home.seek 50
@10:06:12.99 Selecting Cartridge 49
@10:06:13.26 Carousel.seek 49
@10:06:13.80 Carousel.reconfigure :align
@10:06:13.90 Lysis1.in :fast
@10:06:13.97 Lysis1.seek :locked
@10:06:16.47 <Plunge> Plunge.seek :locked
@10:06:16.61 <Air> Air.in :fast
@10:06:16.66 Air.seek :locked
@10:06:17.57 <LRAUV> Carousel.reconfigure :locked
@10:06:17.71 Powering cartridge bus
@10:06:20.34 Gate.setIR 1750,5000,0,6
@10:06:20.47 Gate.setIR 0,5000,0,6
@10:06:20.54 PuckHeater.configure :default
@10:06:20.61 Gate.power :heat,:ON,limit to 5000mA
@10:06:20.80 Mounted lysis Cartridge board#J60008
@10:06:20.84 Using default cartridge heat loss curve for HeatingFunctionInCarousel
@10:06:34.85 -> Cmd.startFiltering
@10:06:35.08 <FILTERING> Preparing to filter lyseHoldBS_bac cartridge in slot 49
@10:06:35.31 <sealing> Air.in :default
@10:06:35.42 Air.seek :sealed
@10:06:35.45 <FILTERING> Priming sample loop w/200ml, bypass w/10ml
@10:06:35.50 SamplePump.setPosition! 0ml
@10:06:35.56 Bypass.open
@10:06:37.15 Intake.open
@10:06:38.28 Sea 30.8m deep (60.2psia)
@10:06:38.30 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@10:06:40.90 Vacuum tare is 52% of full scale at 12C (drifted 1%)
@10:06:41.04 <closeIntake> Intake.close
@10:06:41.07 <closeBypass> Bypass.close
@10:06:41.97 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 59.8psia
@10:06:42.00 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@10:06:46.57 Toroid pressure rose 1% to 60.6psia
@10:06:46.58 Bypass.open
@10:06:47.42 Intake.open
@10:06:49.14 Abs.in :default
@10:06:49.20 Abs.hold
@10:06:49.39 SamplePump.in :start
@10:06:49.46 SamplePump.seek 10ml,1:10
@10:06:50.09 <pumpStarted> SamplePump.configure :prime
@10:07:03.12 <FILTERING> Abs.stop
@10:07:03.18 SamplePump.setPosition! 0ml
@10:07:03.24 Exhaust.open
@10:07:04.81 <closeBypass> Bypass.close
@10:07:06.47 <FILTERING> Abs.in :default
@10:07:06.52 Abs.hold
@10:07:06.64 Sea 36.0m deep (67.9psia)
@10:07:06.80 SamplePump.in :start
@10:07:06.87 SamplePump.seek 200ml,13:50
@10:07:07.49 <pumpStarted> SamplePump.configure :prime
@10:11:32.60 <FILTERING> Abs.stop
@10:11:32.67 Bypass.open
@10:11:33.49 Intake.close
@10:11:34.38 Toroid.in :hold
@10:11:34.43 Toroid.seek :crack
@10:11:36.90 Toroid.seek :cartridge
@10:11:39.02 Toroid.in :default
@10:11:39.08 Toroid.seek :clear
@10:11:43.01 <closeExhaust> Exhaust.close
@10:11:43.04 <closeBypass> Bypass.close
@10:11:43.87 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 88.3psia
@10:11:43.89 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@10:11:48.47 Cartridge pressure fell 2% to 86.7psia
@10:11:48.55 Sampling 1000.0ml
@10:11:48.56 SamplePump.setPosition! 0ml
@10:11:48.63 Exhaust.open
@10:11:49.90 Intake.open
@10:11:51.02 Abs.in :default
@10:11:51.08 Abs.hold
@10:11:51.20 Sea 49.7m deep (88.2psia)
@10:11:51.37 SamplePump.in :start
@10:11:51.43 SamplePump.seek 1000ml,1:07:10
@10:11:52.06 <pumpStarted> SamplePump.configure :sample
@10:11:54.44 <sampling> Cartridge 49 filtering 1/1000ml at 0.60ml/s with 26.8psi vacuum 49.1m deep (87.3psia)
@10:12:54.49 Cartridge 49 filtering 35/1000ml at 0.56ml/s with 23.2psi vacuum 48.1m deep (85.7psia)
@10:13:54.49 Cartridge 49 filtering 68/1000ml at 0.55ml/s with 26.0psi vacuum 47.9m deep (85.5psia)
@10:14:54.49 Cartridge 49 filtering 101/1000ml at 0.55ml/s with 20.3psi vacuum 44.9m deep (81.1psia)
@10:15:54.49 Cartridge 49 filtering 133/1000ml at 0.55ml/s with 28.4psi vacuum 45.1m deep (81.4psia)
@10:16:54.49 Cartridge 49 filtering 166/1000ml at 0.54ml/s with 23.6psi vacuum 48.5m deep (86.4psia)
@10:17:54.49 Cartridge 49 filtering 198/1000ml at 0.54ml/s with 23.2psi vacuum 49.2m deep (87.5psia)
@10:18:54.50 Cartridge 49 filtering 230/1000ml at 0.53ml/s with 19.4psi vacuum 50.8m deep (89.7psia)
@10:19:54.53 Cartridge 49 filtering 262/1000ml at 0.53ml/s with 21.2psi vacuum 51.4m deep (90.6psia)
@10:20:54.49 Cartridge 49 filtering 293/1000ml at 0.52ml/s with 22.2psi vacuum 50.1m deep (88.7psia)
@10:21:54.49 Cartridge 49 filtering 324/1000ml at 0.52ml/s with 22.1psi vacuum 52.5m deep (92.3psia)
@10:22:54.49 Cartridge 49 filtering 354/1000ml at 0.51ml/s with 20.6psi vacuum 46.5m deep (83.4psia)
@10:23:54.49 Cartridge 49 filtering 385/1000ml at 0.51ml/s with 21.2psi vacuum 51.1m deep (90.2psia)
@10:24:54.49 Cartridge 49 filtering 415/1000ml at 0.50ml/s with 25.6psi vacuum 49.6m deep (88.1psia)
@10:25:54.49 Cartridge 49 filtering 444/1000ml at 0.49ml/s with 26.8psi vacuum 42.8m deep (77.9psia)
@10:26:54.49 Cartridge 49 filtering 473/1000ml at 0.49ml/s with 26.3psi vacuum 47.3m deep (84.7psia)
@10:27:54.49 Cartridge 49 filtering 502/1000ml at 0.48ml/s with 24.5psi vacuum 45.8m deep (82.4psia)
@10:28:54.49 Cartridge 49 filtering 531/1000ml at 0.47ml/s with 23.6psi vacuum 47.3m deep (84.6psia)
@10:29:54.53 Cartridge 49 filtering 558/1000ml at 0.46ml/s with 24.2psi vacuum 47.0m deep (84.1psia)
@10:30:54.49 Cartridge 49 filtering 586/1000ml at 0.46ml/s with 24.0psi vacuum 44.3m deep (80.2psia)
@10:31:54.49 Cartridge 49 filtering 613/1000ml at 0.45ml/s with 20.8psi vacuum 54.0m deep (94.5psia)
@10:32:54.49 Cartridge 49 filtering 639/1000ml at 0.44ml/s with 24.1psi vacuum 48.6m deep (86.5psia)
@10:33:54.50 Cartridge 49 filtering 665/1000ml at 0.43ml/s with 20.0psi vacuum 49.2m deep (87.5psia)
@10:34:54.49 Cartridge 49 filtering 691/1000ml at 0.43ml/s with 27.5psi vacuum 41.8m deep (76.4psia)
@10:35:54.49 Cartridge 49 filtering 716/1000ml at 0.42ml/s with 15.9psi vacuum 56.3m deep (97.9psia)
@10:36:54.49 Cartridge 49 filtering 741/1000ml at 0.41ml/s with 24.9psi vacuum 45.4m deep (81.8psia)
@10:37:54.49 Cartridge 49 filtering 765/1000ml at 0.40ml/s with 24.5psi vacuum 46.5m deep (83.4psia)
@10:38:54.49 Cartridge 49 filtering 789/1000ml at 0.40ml/s with 22.3psi vacuum 50.5m deep (89.3psia)
@10:39:54.49 Cartridge 49 filtering 812/1000ml at 0.39ml/s with 21.2psi vacuum 50.6m deep (89.5psia)
@10:40:54.81 Cartridge 49 filtering 835/1000ml at 0.38ml/s with 24.0psi vacuum 49.4m deep (87.6psia)
@10:41:54.49 Cartridge 49 filtering 858/1000ml at 0.37ml/s with 20.6psi vacuum 49.4m deep (87.7psia)
@10:42:54.49 Cartridge 49 filtering 880/1000ml at 0.37ml/s with 26.6psi vacuum 45.7m deep (82.3psia)
@10:43:54.49 Cartridge 49 filtering 901/1000ml at 0.36ml/s with 21.9psi vacuum 54.1m deep (94.7psia)
@10:44:54.49 Cartridge 49 filtering 922/1000ml at 0.35ml/s with 27.5psi vacuum 45.6m deep (82.0psia)
@10:45:54.49 Cartridge 49 filtering 943/1000ml at 0.35ml/s with 26.1psi vacuum 47.6m deep (85.1psia)
@10:46:54.49 Cartridge 49 filtering 963/1000ml at 0.34ml/s with 20.6psi vacuum 47.8m deep (85.3psia)
@10:47:54.49 Cartridge 49 filtering 983/1000ml at 0.33ml/s with 24.2psi vacuum 50.0m deep (88.6psia)
@10:48:46.22 <FILTERING> Abs.stop
@10:48:46.34 <closeIntake> Intake.close
@10:48:46.39 <closeExhaust> Exhaust.close
@10:48:46.58 <FILTERING> Toroid.in :hold
@10:48:46.64 Toroid.seek :bypass
@10:48:50.24 Toroid.in :default
@10:48:50.30 Toroid.seek :clear
@10:48:53.88 Sampled  1000.0ml
@10:48:55.30 <LRAUV> -> Cmd.startProcessing
@10:48:55.66 <PROCESSING> Handoff.select :waste
@10:48:55.79 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@10:49:02.38 Syringe tare is 50% of full scale at 14C (drifted -1%)
@10:49:02.41 PinPort.in :locking
@10:49:02.48 PinPort.seek :locked
@10:49:04.92 <posArchive> Archive.in :fast
@10:49:04.99 <posLyS2> Lysis2.in :fast
@10:49:05.04 <PROCESSING> Lysis1.seek 1250uL
@10:49:05.10 <posArchive> Archive.seek :empty
@10:49:05.19 <posLyS2> Lysis2.seek :empty
@10:49:13.59 <PROCESSING> Evacuating puck with filter bubble point of 50psi
@10:49:13.61 PinPort.seek :locked
@10:49:13.70 Handoff.select :waste
@10:49:13.73 Plunge.seek :puckBottom
@10:49:15.04 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@10:49:25.62 Air syringe pumping up to 1 strokes to reach 8psi
@10:49:25.63 Air stroke #1 of 1
@10:49:25.72 Air.in :pressurize
@10:49:25.78 Air.seek :pressurized
@10:49:30.86 -0.1psi after pressurize
@10:49:30.88 Air.in :compress
@10:49:30.95 Air.seek :evac
@10:49:37.98 0.5psi after compress
@10:49:48.56 -0.0psi after compress (stable)
@10:49:48.58 Air.in :default
@10:49:48.64 Air.hold
@10:49:48.82 at 0.1psi Opened to waste
@10:49:48.84 Handoff.select :blocked
@10:49:48.97 Plunge.seek :puckTop
@10:49:49.86 Air syringe pumping up to 8 strokes to reach 50psi
@10:49:49.87 Air stroke #1 of 8
@10:49:49.97 Air.in :compress
@10:49:50.03 Air.seek :evac
@10:49:50.15 8.0psi after compress
@10:50:00.72 8.6psi after compress (stable)
@10:50:00.75 Air.in :gulp
@10:50:00.82 Air.seek :topVent
@10:50:07.41 8.5psi after gulp
@10:50:18.98 8.7psi after gulp (stable)
@10:50:18.99 Air stroke #2 of 8
@10:50:19.08 Air.in :pressurize
@10:50:19.14 Air.seek :pressurized
@10:50:25.58 8.2psi after pressurize
@10:50:25.60 Air.in :compress
@10:50:25.66 Air.seek :evac
@10:50:32.69 21.3psi after compress
@10:50:46.27 25.8psi after compress (stable)
@10:50:46.29 Air.in :gulp
@10:50:46.35 Air.seek :topVent
@10:50:52.94 25.9psi after gulp
@10:50:59.51 25.8psi after gulp (stable)
@10:50:59.52 Air stroke #3 of 8
@10:50:59.62 Air.in :pressurize
@10:50:59.68 Air.seek :pressurized
@10:51:06.12 25.4psi after pressurize
@10:51:06.14 Air.in :compress
@10:51:06.20 Air.seek :evac
@10:51:13.24 35.4psi after compress
@10:51:26.31 40.2psi after compress (stable)
@10:51:26.33 Air.in :gulp
@10:51:26.40 Air.seek :topVent
@10:51:33.00 40.0psi after gulp
@10:51:39.07 40.2psi after gulp (stable)
@10:51:39.08 Air stroke #4 of 8
@10:51:39.17 Air.in :pressurize
@10:51:39.23 Air.seek :pressurized
@10:51:45.66 39.6psi after pressurize
@10:51:45.68 Air.in :compress
@10:51:45.76 Air.seek :evac
@10:51:52.79 47.7psi after compress
@10:51:53.04 Reached 49.0 of 50.0psi after pumping 7107uL (4.00 strokes)
@10:51:53.05 Air.in :default
@10:51:53.12 Air.hold
@10:51:53.20 Plunge.seek :puckBottom
@10:51:54.09 delay 3 seconds
@10:51:57.11 Handoff.select :waste
@10:51:57.23 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@10:52:12.81 delay 1:00
@10:53:12.84 Handoff.select :blocked
@10:53:12.99 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@10:53:35.56 Handoff.select :waste
@10:53:35.69 2.0psi at evacEnd
@10:53:35.70 Handoff.select :blocked
@10:53:35.84 Plunge.seek :puckTop
@10:53:36.73 Air.in :fast
@10:53:36.79 Air.seek :topVent
@10:53:43.43 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi
@10:53:50.00 Stable @17.2psi after Handoff blocked
@10:53:50.02 Air syringe pumping up to 8 strokes to reach 35psi
@10:53:50.04 Air stroke #1 of 8
@10:53:50.13 Air.in :pressurize
@10:53:50.19 Air.seek :pressurized
@10:53:56.63 16.9psi after pressurize
@10:53:56.65 Air.in :compress
@10:53:56.72 Air.seek :evac
@10:54:03.75 25.9psi after compress
@10:54:16.32 29.6psi after compress (stable)
@10:54:16.34 Air.in :gulp
@10:54:16.40 Air.seek :topVent
@10:54:23.00 29.3psi after gulp
@10:54:28.93 29.7psi after gulp (stable)
@10:54:28.94 Air stroke #2 of 8
@10:54:29.04 Air.in :pressurize
@10:54:29.10 Air.seek :pressurized
@10:54:35.53 29.3psi after pressurize
@10:54:35.55 Air.in :compress
@10:54:35.62 Air.seek :evac
@10:54:42.28 Reached 35.6 of 35.0psi after pumping 3531uL (1.99 strokes)
@10:54:42.29 Air.in :default
@10:54:42.36 Air.hold
@10:54:42.44 Plunge.seek :puckBottom
@10:54:43.33 delay 3 seconds
@10:54:46.35 Handoff.select :waste
@10:54:46.47 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@10:54:53.04 delay 1:00
@10:55:53.07 Handoff.select :blocked
@10:55:53.20 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@10:56:15.78 Handoff.select :waste
@10:56:15.91 2.0psi at evacEnd
@10:56:15.93 Plunge.seek :pTop
@10:56:16.82 delay 5 seconds
@10:56:21.85 Air.in :glide
@10:56:21.90 Air.seek :fullVent
@10:56:23.98 delay 2 seconds
@10:56:26.00 Plunge.seek :pBottom
@10:56:26.89 Seawater evacuated, Pressure above/below filter released.
@10:56:26.91 Plunge.seek :locked
@10:56:28.22 Handoff.select :waste
@10:56:28.31 Lysis1.in :slow
@10:56:28.38 Lysis1.seek 950uL
@10:56:42.56 delay 5 seconds
@10:56:47.58 Handoff.select :blocked
@10:56:47.72 Plunge.seek :puckTop
@10:56:48.64 Lysis1.seek 350uL
@10:57:16.83 delay 3 seconds
@10:57:19.91 Air.in mix with 2.32937 parts Lysis1
@10:57:20.45 <Airmixer> Air.seek :evac
@10:57:20.48 <PROCESSING> Lysis1.seek 190uL
@10:57:28.32 Air.in :fast
@10:57:28.39 Air.seek :full
@10:57:35.03 Lysis1.seek :empty
@10:57:44.19 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @22.8psi
@10:57:44.21 Plunge.seek :puckBottom
@10:57:45.21 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @17.8psi
@10:57:45.22 Polling Can every 2:00
@10:57:45.77 PuckHeater.configure :default #ambient 14.2C
@10:57:45.82 PuckHeater.seek 71.6C
@10:59:15.82 PuckHeater.hold 2:00,71.6C
@11:01:15.53 Polling Can every 2:00
@11:01:16.07 PuckHeater.configure :default #ambient 14.3C
@11:01:16.13 PuckHeater.seek 70.5C
@11:01:16.27 PuckHeater.hold 2:00,70.5C
@11:03:16.00 Polling Can every 2:00
@11:03:16.54 PuckHeater.configure :default #ambient 14.3C
@11:03:16.60 PuckHeater.hold 18:00,69.5C
@11:21:12.90 Polling Can every 2:00
@11:21:13.44 PuckHeater.configure :default #ambient 14.2C
@11:21:13.50 PuckHeater.seek 105.3C
@11:22:15.81 PuckHeater.hold 2:00,105.3C
@11:24:15.44 Polling Can every 2:00
@11:24:15.98 PuckHeater.configure :default #ambient 14.3C
@11:24:16.04 PuckHeater.seek 104.3C
@11:24:16.19 PuckHeater.hold 2:00,104.3C
@11:26:15.82 Polling Can every 2:00
@11:26:16.36 PuckHeater.configure :default #ambient 14.3C
@11:26:16.42 PuckHeater.hold 2:00,103.2C
@11:28:16.14 delay 5:00
@11:33:16.21 Lysate cooked @11.9psi
@11:33:16.23 Plunge.seek :puckTop
@11:33:17.13 Air syringe pumping up to 5 strokes to reach 40psi
@11:33:17.14 Air stroke #1 of 5
@11:33:17.23 Air.in :pressurize
@11:33:17.29 Air.seek :pressurized
@11:33:23.72 11.5psi after pressurize
@11:33:23.74 Air.in :compress
@11:33:23.81 Air.seek :evac
@11:33:28.65 Reached 41.0 of 40.0psi after pumping 1653uL (0.93 strokes)
@11:33:28.67 Air.in :default
@11:33:28.73 Air.hold
@11:33:28.81 delay 2 seconds
@11:33:30.83 Air.in :fast
@11:33:30.88 Air.seek :full
@11:33:37.08 Plunge.seek :puckBottom
@11:33:38.02 Lysis1.seek 138uL
@11:33:44.61 delay 2 seconds
@11:33:46.64 Plunge.seek :puckTop
@11:33:47.53 Lysis1.seek :empty
@11:33:54.13 Plunge.seek :puckBottom
@11:33:55.21 Lysis1.in :extract
@11:33:55.28 Lysis1.seek 675uL,nil
@11:33:57.04 <pump> Air.in :pressurize
@11:33:57.09 Air.seek :pressurized
@11:34:03.53 11.1psi after pressurize
@11:34:03.55 Air.in :compressLysate
@11:34:03.63 Air.seek :evac
@11:34:18.16 Reached 15.6 of 15.5psi after pumping 1592uL (0.90 strokes)
@11:34:18.18 Air.in :default
@11:34:18.25 Air.hold
@11:34:19.09 Air.in :compressLysate
@11:34:19.15 Air.seek :evac
@11:34:29.81 Air.in :default
@11:34:29.88 Air.hold
@11:34:30.08 <PROCESSING> Pulled 675uL lysate @14.1psi
@11:34:30.23 Lysis1.seek 800uL,nil
@11:34:30.44 <pump> Air.in :compressLysate
@11:34:30.49 Air.seek :evac
@11:34:33.20 12.5psi after compress
@11:34:43.77 11.9psi after compress (stable)
@11:34:49.02 Air.in :default
@11:34:49.07 <PROCESSING> Lysis1.in :default
@11:34:49.09 <pump> Air.hold
@11:34:49.19 <PROCESSING> Lysis1.hold
@11:34:49.44 Air.in :glide
@11:34:49.50 Air.seek :fullVent
@11:34:51.30 Handoff.select :waste
@11:34:51.43 Recovered 743uL lysate @12.0psi
@11:34:51.44 delay 3 seconds
@11:34:54.52 After 3 delay Recovered 743uL lysate @-0.2psi
@11:34:55.71 <LRAUV> -> Cmd.stop
@11:34:55.81 <closeHandoff> Handoff.select :close
@11:34:55.95 <LRAUV> Gate.power :heat,:OFF
@11:34:55.97 Gate.power :cartridge,:OFF
@11:34:56.06 PinPort.in :unlocking
@11:34:56.12 PinPort.seek :clear
@11:34:58.55 Carousel.in :default
@11:34:58.66 Carousel.home.seek 49
@11:34:58.97 Plunge.seek :locked
@11:35:00.34 <Archive> Archive.in :unlocking
@11:35:00.42 Archive.seek :clear
@11:35:00.55 <Air> Air.in :unlocking
@11:35:00.68 Air.seek :clear
@11:35:00.76 <Lysis1> Lysis1.in :unlocking
@11:35:00.89 Lysis1.seek :clear
@11:35:00.97 <Lysis2> Lysis2.in :unlocking
@11:35:01.04 Lysis2.seek :clear
@11:35:05.79 <LRAUV> Gate.power :motor,:OFF
@11:35:05.81 Gate.power :analytic1,:OFF
@11:35:05.84 Gate.power :analytic2,:OFF
@11:35:05.86 Gate.power :analytic3,:OFF
@11:35:05.89 Gate.power :core,:OFF
@11:35:05.91 Gate.power :main,:OFF
@11:35:05.93 Safely stopped and ready to power off
##-- file closed 2025-12-05T22:54:52.215Z

##-- file opened 2025-12-05T22:54:52.241Z
@14:54:52.71 -> showStatus
@14:54:54.73 -> Cmd.loadCartridge -6
@14:54:56.76 -> Cmd.stop
@14:54:56.80 ready to power off
##-- file closed 2025-12-05T23:41:08.099Z

##-- file opened 2025-12-05T23:41:08.102Z
@15:41:08.27 -> showStatus
@15:41:10.29 -> Cmd.loadCartridge -15
@15:41:10.38 Gate.power :main,:ON,limit to 5000mA
@15:41:10.40 Gate.power :analytic1,:ON,limit to 4000mA
@15:41:10.42 Gate.power :analytic2,:ON,limit to 4000mA
@15:41:10.44 Gate.power :analytic3,:ON,limit to 4000mA
@15:41:10.50 Powering core bus
@15:41:13.20 Plunge.configure :default
@15:41:13.28 SamplePump.configure :default
@15:41:13.37 Toroid.configure :default
@15:41:13.45 Carousel.configure :default
@15:41:13.54 Abs.configure :default
@15:41:13.61 Archive.configure :default
@15:41:13.69 Air.configure :default
@15:41:13.75 Lysis1.configure :default
@15:41:13.81 Lysis2.configure :default
@15:41:13.89 PinPort.configure :default
@15:41:13.95 WARNING:  Multiple controller firmware versions in use
[:sampler0, :syringe1, :sampler1, :valve0, :ambient0, :valve1, :syringe0]=>4.93, [:carousel0]=>4.94
@15:41:13.98 Gate.power :motor,:ON,limit to 5000mA
@15:41:14.11 <Plunge_home> Plunge.home.jog 0
@15:41:14.69 <PinPort_home> PinPort.home.jog 0
@15:41:14.74 <Lysis2_home> Lysis2.home.jog 0
@15:41:14.76 <Lysis1_home> Lysis1.home.jog 0
@15:41:14.79 <Air_home> Air.home.jog 0
@15:41:14.82 <Carousel_home> Carousel.home.jog 0
@15:41:14.85 <Toroid_home> Toroid.home.jog 0
@15:41:14.88 <Archive_home> Archive.home.jog 0
@15:41:15.47 <Plunge_home> Plunge.in :homing
@15:41:15.68 Plunge.jog 1528
@15:41:15.84 <PinPort_home> PinPort.in :homing
@15:41:15.87 <Lysis2_home> Lysis2.in :homing
@15:41:15.90 <Lysis1_home> Lysis1.in :homing
@15:41:15.93 <Air_home> Air.in :homing
@15:41:15.96 <Archive_home> Archive.in :homing
@15:41:15.99 <Toroid_home> Toroid.in :homing
@15:41:16.15 <Lysis2_home> Lysis2.jog 301
@15:41:16.20 <PinPort_home> PinPort.jog -1203
@15:41:16.27 <Lysis1_home> Lysis1.jog 306
@15:41:16.32 <Air_home> Air.jog 365
@15:41:16.36 <Archive_home> Archive.jog 322
@15:41:16.43 <Toroid_home> Toroid.jog 1301
@15:41:16.89 <Lysis2_home> Lysis2.setRawPosition! 50
@15:41:16.95 Lysis2.seek :home
@15:41:17.04 <Plunge_home> Plunge.setRawPosition! 50
@15:41:17.11 Plunge.seek :home
@15:41:17.20 <Lysis1_home> Lysis1.setRawPosition! 50
@15:41:17.24 <Archive_home> Archive.setRawPosition! 50
@15:41:17.28 <Air_home> Air.setRawPosition! 0
@15:41:17.39 <Lysis1_home> Lysis1.seek :home
@15:41:17.44 <Air_home> Air.seek :home
@15:41:17.47 <Archive_home> Archive.seek :home
@15:41:17.67 <Lysis2_home> Lysis2.in :default
@15:41:17.70 <Air_home> Air.in :default
@15:41:17.73 <Plunge_home> Plunge.in :default
@15:41:17.90 <Archive_home> Archive.in :default
@15:41:17.93 <Lysis1_home> Lysis1.in :default
@15:41:18.13 <PinPort_home> PinPort.setRawPosition! 0
@15:41:18.18 PinPort.seek :home
@15:41:18.28 PinPort.in :default
@15:41:20.89 <Toroid_home> Toroid.setRawPosition! 0
@15:41:20.95 Toroid.seek :home
@15:41:21.02 Toroid.in :default
@15:41:21.09 <clearToroid> Toroid.seek :clear
@15:41:21.51 <LRAUV> PinPort.in :unlocking
@15:41:21.64 PinPort.seek :clear
@15:41:25.03 Air.seek :clear
@15:41:25.92 Lysis1.seek :clear
@15:41:26.71 Lysis2.seek :clear
@15:41:27.50 Archive.seek :clear
@15:41:28.29 Carousel.in :default
@15:41:28.40 Carousel.home.seek 49
@15:41:28.66 Selecting Cartridge 48
@15:41:28.93 Carousel.seek 48
@15:41:29.46 Carousel.reconfigure :align
@15:41:29.57 Lysis1.in :fast
@15:41:29.64 Lysis1.seek :locked
@15:41:32.12 <Plunge> Plunge.seek :locked
@15:41:32.26 <Air> Air.in :fast
@15:41:32.32 Air.seek :locked
@15:41:33.23 <LRAUV> Carousel.reconfigure :locked
@15:41:33.37 Powering cartridge bus
@15:41:36.00 Gate.setIR 1750,5000,0,6
@15:41:36.12 Gate.setIR 0,5000,0,6
@15:41:36.19 PuckHeater.configure :default
@15:41:36.26 Gate.power :heat,:ON,limit to 5000mA
@15:41:36.44 Mounted lysis Cartridge board#J59998
@15:41:36.48 Using default cartridge heat loss curve for HeatingFunctionInCarousel
@15:41:50.57 -> Cmd.startFiltering
@15:41:50.82 <FILTERING> Preparing to filter lyseHoldBS_bac cartridge in slot 48
@15:41:51.06 <sealing> Air.in :default
@15:41:51.16 Air.seek :sealed
@15:41:51.25 <FILTERING> Priming sample loop w/200ml, bypass w/10ml
@15:41:51.26 SamplePump.setPosition! 0ml
@15:41:51.31 Bypass.open
@15:41:52.91 Intake.open
@15:41:54.04 Sea 83.7m deep (138.4psia)
@15:41:54.06 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@15:41:56.66 Vacuum tare is 52% of full scale at 12C (drifted 1%)
@15:41:56.84 <closeIntake> Intake.close
@15:41:56.89 <closeBypass> Bypass.close
@15:41:57.79 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 138.8psia
@15:41:57.81 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@15:42:02.39 Toroid pressure fell 2% to 136.3psia
@15:42:02.40 Bypass.open
@15:42:03.27 Intake.open
@15:42:05.06 Abs.in :default
@15:42:05.11 Abs.hold
@15:42:05.32 SamplePump.in :start
@15:42:05.39 SamplePump.seek 10ml,1:10
@15:42:06.01 <pumpStarted> SamplePump.configure :prime
@15:42:19.04 <FILTERING> Abs.stop
@15:42:19.09 SamplePump.setPosition! 0ml
@15:42:19.14 Exhaust.open
@15:42:20.77 <closeBypass> Bypass.close
@15:42:22.42 <FILTERING> Abs.in :default
@15:42:22.47 Abs.hold
@15:42:22.58 Sea 78.4m deep (130.6psia)
@15:42:22.79 SamplePump.in :start
@15:42:22.85 SamplePump.seek 200ml,13:50
@15:42:23.48 <pumpStarted> SamplePump.configure :prime
@15:46:48.55 <FILTERING> Abs.stop
@15:46:48.62 Bypass.open
@15:46:49.44 Intake.close
@15:46:50.57 Toroid.in :hold
@15:46:50.63 Toroid.seek :crack
@15:46:53.10 Toroid.seek :cartridge
@15:46:55.22 Toroid.in :default
@15:46:55.28 Toroid.seek :clear
@15:46:59.21 <closeExhaust> Exhaust.close
@15:46:59.25 <closeBypass> Bypass.close
@15:47:00.11 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 137.5psia
@15:47:00.13 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@15:47:04.70 Cartridge pressure fell 1% to 136.5psia
@15:47:04.78 Sampling 1000.0ml
@15:47:04.79 SamplePump.setPosition! 0ml
@15:47:05.21 Exhaust.open
@15:47:06.13 Intake.open
@15:47:07.35 Abs.in :default
@15:47:07.40 Abs.hold
@15:47:07.53 Sea 84.4m deep (139.5psia)
@15:47:07.69 SamplePump.in :start
@15:47:07.76 SamplePump.seek 1000ml,1:07:10
@15:47:08.39 <pumpStarted> SamplePump.configure :sample
@15:47:10.78 <sampling> Cartridge 48 filtering 1/1000ml at 0.59ml/s with 18.7psi vacuum 90.4m deep (148.3psia)
@15:48:10.82 Cartridge 48 filtering 35/1000ml at 0.55ml/s with 14.7psi vacuum 86.1m deep (142.0psia)
@15:49:10.83 Cartridge 48 filtering 68/1000ml at 0.55ml/s with 21.2psi vacuum 85.9m deep (141.6psia)
@15:50:10.82 Cartridge 48 filtering 100/1000ml at 0.55ml/s with 22.5psi vacuum 78.6m deep (130.9psia)
@15:51:10.83 Cartridge 48 filtering 133/1000ml at 0.55ml/s with 21.4psi vacuum 85.7m deep (141.4psia)
@15:52:10.82 Cartridge 48 filtering 166/1000ml at 0.55ml/s with 29.9psi vacuum 75.0m deep (125.6psia)
@15:53:10.83 Cartridge 48 filtering 199/1000ml at 0.54ml/s with 28.0psi vacuum 73.7m deep (123.6psia)
@15:54:10.87 Cartridge 48 filtering 231/1000ml at 0.54ml/s with 20.6psi vacuum 83.8m deep (138.5psia)
@15:55:10.83 Cartridge 48 filtering 263/1000ml at 0.54ml/s with 27.1psi vacuum 74.7m deep (125.0psia)
@15:56:10.82 Cartridge 48 filtering 295/1000ml at 0.53ml/s with 16.7psi vacuum 92.1m deep (150.9psia)
@15:57:10.82 Cartridge 48 filtering 327/1000ml at 0.53ml/s with 28.4psi vacuum 65.8m deep (111.9psia)
@15:58:10.82 Cartridge 48 filtering 358/1000ml at 0.53ml/s with 24.2psi vacuum 81.2m deep (134.6psia)
@15:59:10.82 Cartridge 48 filtering 390/1000ml at 0.52ml/s with 25.1psi vacuum 74.7m deep (125.1psia)
@16:00:10.82 Cartridge 48 filtering 421/1000ml at 0.52ml/s with 31.5psi vacuum 73.1m deep (122.7psia)
@16:01:10.82 Cartridge 48 filtering 452/1000ml at 0.52ml/s with 15.6psi vacuum 93.5m deep (152.9psia)
@16:02:10.82 Cartridge 48 filtering 483/1000ml at 0.52ml/s with 16.8psi vacuum 84.8m deep (140.0psia)
@16:03:10.85 Cartridge 48 filtering 514/1000ml at 0.52ml/s with 15.5psi vacuum 92.2m deep (151.0psia)
@16:04:10.82 Cartridge 48 filtering 545/1000ml at 0.51ml/s with 15.6psi vacuum 88.1m deep (144.9psia)
@16:05:10.82 Cartridge 48 filtering 576/1000ml at 0.51ml/s with 20.0psi vacuum 83.7m deep (138.4psia)
@16:06:10.82 Cartridge 48 filtering 606/1000ml at 0.51ml/s with 20.3psi vacuum 83.3m deep (137.7psia)
@16:07:10.82 Cartridge 48 filtering 637/1000ml at 0.51ml/s with 26.9psi vacuum 77.2m deep (128.7psia)
@16:08:10.82 Cartridge 48 filtering 667/1000ml at 0.50ml/s with 19.4psi vacuum 79.6m deep (132.4psia)
@16:09:10.82 Cartridge 48 filtering 697/1000ml at 0.50ml/s with 16.7psi vacuum 88.8m deep (145.9psia)
@16:10:10.83 Cartridge 48 filtering 726/1000ml at 0.50ml/s with 34.6psi vacuum 70.9m deep (119.4psia)
@16:11:10.87 Cartridge 48 filtering 756/1000ml at 0.49ml/s with 21.0psi vacuum 86.1m deep (142.0psia)
@16:12:10.82 Cartridge 48 filtering 786/1000ml at 0.49ml/s with 28.9psi vacuum 74.7m deep (125.2psia)
@16:13:10.82 Cartridge 48 filtering 815/1000ml at 0.49ml/s with 28.1psi vacuum 75.6m deep (126.4psia)
@16:14:10.82 Cartridge 48 filtering 844/1000ml at 0.49ml/s with 16.9psi vacuum 92.5m deep (151.4psia)
@16:15:10.82 Cartridge 48 filtering 873/1000ml at 0.48ml/s with 20.6psi vacuum 72.0m deep (121.2psia)
@16:16:11.12 Cartridge 48 filtering 902/1000ml at 0.48ml/s with 20.1psi vacuum 81.9m deep (135.7psia)
@16:17:10.82 Cartridge 48 filtering 930/1000ml at 0.47ml/s with 18.0psi vacuum 81.0m deep (134.4psia)
@16:18:10.82 Cartridge 48 filtering 958/1000ml at 0.47ml/s with 24.2psi vacuum 83.2m deep (137.7psia)
@16:19:10.82 Cartridge 48 filtering 986/1000ml at 0.47ml/s with 17.0psi vacuum 84.6m deep (139.8psia)
@16:19:41.30 <FILTERING> Abs.stop
@16:19:41.43 <closeIntake> Intake.close
@16:19:41.48 <closeExhaust> Exhaust.close
@16:19:41.66 <FILTERING> Toroid.in :hold
@16:19:41.72 Toroid.seek :bypass
@16:19:45.31 Toroid.in :default
@16:19:45.37 Toroid.seek :clear
@16:19:48.96 Sampled  1000.0ml
@16:19:50.30 <LRAUV> -> Cmd.startProcessing
@16:19:50.65 <PROCESSING> Handoff.select :waste
@16:19:50.78 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@16:20:07.87 Syringe tare is 50% of full scale at 13C (drifted -1%)
@16:20:07.89 PinPort.in :locking
@16:20:07.97 PinPort.seek :locked
@16:20:10.40 <posArchive> Archive.in :fast
@16:20:10.46 <posLyS2> Lysis2.in :fast
@16:20:10.51 <PROCESSING> Lysis1.seek 1250uL
@16:20:11.03 <posArchive> Archive.seek :empty
@16:20:11.14 <posLyS2> Lysis2.seek :empty
@16:20:19.55 <PROCESSING> Evacuating puck with filter bubble point of 50psi
@16:20:19.57 PinPort.seek :locked
@16:20:19.66 Handoff.select :waste
@16:20:19.69 Plunge.seek :puckBottom
@16:20:21.04 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@16:20:27.62 Air syringe pumping up to 1 strokes to reach 8psi
@16:20:27.63 Air stroke #1 of 1
@16:20:27.72 Air.in :pressurize
@16:20:27.78 Air.seek :pressurized
@16:20:32.85 -0.3psi after pressurize
@16:20:32.87 Air.in :compress
@16:20:32.93 Air.seek :evac
@16:20:39.95 1.1psi after compress
@16:20:51.02 0.2psi after compress (stable)
@16:20:51.03 Air.in :default
@16:20:51.10 Air.hold
@16:20:51.27 at 0.3psi Opened to waste
@16:20:51.29 Handoff.select :blocked
@16:20:51.43 Plunge.seek :puckTop
@16:20:52.33 Air syringe pumping up to 8 strokes to reach 50psi
@16:20:52.34 Air stroke #1 of 8
@16:20:52.44 Air.in :compress
@16:20:52.50 Air.seek :evac
@16:20:52.63 6.1psi after compress
@16:21:03.20 6.5psi after compress (stable)
@16:21:03.22 Air.in :gulp
@16:21:03.29 Air.seek :topVent
@16:21:09.87 6.5psi after gulp
@16:21:15.44 6.7psi after gulp (stable)
@16:21:15.45 Air stroke #2 of 8
@16:21:15.54 Air.in :pressurize
@16:21:15.60 Air.seek :pressurized
@16:21:22.03 6.3psi after pressurize
@16:21:22.05 Air.in :compress
@16:21:22.12 Air.seek :evac
@16:21:29.16 19.6psi after compress
@16:21:42.23 24.1psi after compress (stable)
@16:21:42.25 Air.in :gulp
@16:21:42.32 Air.seek :topVent
@16:21:48.98 23.9psi after gulp
@16:21:55.05 24.2psi after gulp (stable)
@16:21:55.06 Air stroke #3 of 8
@16:21:55.16 Air.in :pressurize
@16:21:55.22 Air.seek :pressurized
@16:22:01.66 23.8psi after pressurize
@16:22:01.68 Air.in :compress
@16:22:01.75 Air.seek :evac
@16:22:08.77 34.5psi after compress
@16:22:21.84 38.6psi after compress (stable)
@16:22:21.86 Air.in :gulp
@16:22:21.93 Air.seek :topVent
@16:22:28.82 38.6psi after gulp
@16:22:34.89 38.9psi after gulp (stable)
@16:22:34.90 Air stroke #4 of 8
@16:22:34.99 Air.in :pressurize
@16:22:35.05 Air.seek :pressurized
@16:22:41.48 38.2psi after pressurize
@16:22:41.50 Air.in :compress
@16:22:41.57 Air.seek :evac
@16:22:48.60 46.7psi after compress
@16:22:51.86 Reached 50.1 of 50.0psi after pumping 7107uL (4.00 strokes)
@16:22:51.87 Air.in :default
@16:22:51.94 Air.hold
@16:22:52.02 Plunge.seek :puckBottom
@16:22:52.91 delay 3 seconds
@16:22:55.93 Handoff.select :waste
@16:22:56.06 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@16:23:06.14 delay 1:00
@16:24:06.17 Handoff.select :blocked
@16:24:06.30 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@16:24:32.37 Handoff.select :waste
@16:24:32.49 3.5psi at evacEnd
@16:24:32.50 Handoff.select :blocked
@16:24:32.64 Plunge.seek :puckTop
@16:24:33.53 Air.in :fast
@16:24:33.60 Air.seek :topVent
@16:24:40.24 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi
@16:24:46.81 Stable @17.8psi after Handoff blocked
@16:24:46.83 Air syringe pumping up to 8 strokes to reach 35psi
@16:24:46.84 Air stroke #1 of 8
@16:24:46.94 Air.in :pressurize
@16:24:47.00 Air.seek :pressurized
@16:24:53.45 17.3psi after pressurize
@16:24:53.47 Air.in :compress
@16:24:53.54 Air.seek :evac
@16:25:00.56 26.3psi after compress
@16:25:13.63 29.8psi after compress (stable)
@16:25:13.65 Air.in :gulp
@16:25:13.72 Air.seek :topVent
@16:25:20.32 29.6psi after gulp
@16:25:25.89 29.9psi after gulp (stable)
@16:25:25.90 Air stroke #2 of 8
@16:25:25.99 Air.in :pressurize
@16:25:26.05 Air.seek :pressurized
@16:25:32.82 29.5psi after pressurize
@16:25:32.84 Air.in :compress
@16:25:32.91 Air.seek :evac
@16:25:39.55 Reached 35.3 of 35.0psi after pumping 3530uL (1.99 strokes)
@16:25:39.57 Air.in :default
@16:25:39.64 Air.hold
@16:25:39.72 Plunge.seek :puckBottom
@16:25:40.61 delay 3 seconds
@16:25:43.63 Handoff.select :waste
@16:25:43.76 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@16:25:50.33 delay 1:00
@16:26:50.36 Handoff.select :blocked
@16:26:50.49 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@16:27:13.06 Handoff.select :waste
@16:27:13.18 1.9psi at evacEnd
@16:27:13.19 Plunge.seek :pTop
@16:27:14.09 delay 5 seconds
@16:27:19.12 Air.in :glide
@16:27:19.17 Air.seek :fullVent
@16:27:21.26 delay 2 seconds
@16:27:23.28 Plunge.seek :pBottom
@16:27:24.17 Seawater evacuated, Pressure above/below filter released.
@16:27:24.19 Plunge.seek :locked
@16:27:25.50 Handoff.select :waste
@16:27:25.59 Lysis1.in :slow
@16:27:25.65 Lysis1.seek 950uL
@16:27:39.83 delay 5 seconds
@16:27:44.85 Handoff.select :blocked
@16:27:44.99 Plunge.seek :puckTop
@16:27:45.91 Lysis1.seek 350uL
@16:28:14.09 delay 3 seconds
@16:28:17.17 Air.in mix with 2.32937 parts Lysis1
@16:28:17.67 <Airmixer> Air.seek :evac
@16:28:17.71 <PROCESSING> Lysis1.seek 190uL
@16:28:25.52 Air.in :fast
@16:28:25.59 Air.seek :full
@16:28:32.23 Lysis1.seek :empty
@16:28:41.37 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @18.3psi
@16:28:41.39 Plunge.seek :puckBottom
@16:28:42.39 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @14.4psi
@16:28:42.41 Polling Can every 2:00
@16:28:42.96 PuckHeater.configure :default #ambient 13.0C
@16:28:43.02 PuckHeater.seek 71.6C
@16:30:20.25 PuckHeater.hold 2:00,71.6C
@16:32:20.53 Polling Can every 2:00
@16:32:21.07 PuckHeater.configure :default #ambient 13.1C
@16:32:21.13 PuckHeater.seek 70.6C
@16:32:21.27 PuckHeater.hold 2:00,70.6C
@16:34:21.64 Polling Can every 2:00
@16:34:22.18 PuckHeater.configure :default #ambient 13.1C
@16:34:22.24 PuckHeater.hold 18:00,69.5C
@16:52:23.62 Polling Can every 2:00
@16:52:24.15 PuckHeater.configure :default #ambient 12.9C
@16:52:24.21 PuckHeater.seek 105.4C
@16:53:33.09 PuckHeater.hold 2:00,105.4C
@16:55:33.24 Polling Can every 2:00
@16:55:33.78 PuckHeater.seek 104.4C
@16:55:34.32 PuckHeater.hold 2:00,104.4C
@16:57:34.52 Polling Can every 2:00
@16:57:35.06 PuckHeater.configure :default #ambient 13.0C
@16:57:35.12 PuckHeater.hold 2:00,103.3C
@16:59:35.34 delay 5:00
@17:04:35.40 Lysate cooked @17.0psi
@17:04:35.42 Plunge.seek :puckTop
@17:04:36.33 Air syringe pumping up to 5 strokes to reach 40psi
@17:04:36.34 Air stroke #1 of 5
@17:04:36.43 Air.in :pressurize
@17:04:36.49 Air.seek :pressurized
@17:04:42.92 15.1psi after pressurize
@17:04:42.94 Air.in :compress
@17:04:43.01 Air.seek :evac
@17:04:49.30 Reached 41.0 of 40.0psi after pumping 1734uL (0.98 strokes)
@17:04:49.32 Air.in :default
@17:04:49.39 Air.hold
@17:04:49.47 delay 2 seconds
@17:04:51.49 Air.in :fast
@17:04:51.54 Air.seek :full
@17:04:58.04 Plunge.seek :puckBottom
@17:04:58.99 Lysis1.seek 138uL
@17:05:05.59 delay 2 seconds
@17:05:07.61 Plunge.seek :puckTop
@17:05:08.50 Lysis1.seek :empty
@17:05:15.11 Plunge.seek :puckBottom
@17:05:16.24 Lysis1.in :extract
@17:05:16.31 Lysis1.seek 675uL,nil
@17:05:18.60 <pump> Air.in :pressurize
@17:05:18.66 Air.seek :pressurized
@17:05:25.13 12.7psi after pressurize
@17:05:25.15 Air.in :compressLysate
@17:05:25.22 Air.seek :evac
@17:05:52.96 12.8psi after compress
@17:06:03.53 14.0psi after compress (stable)
@17:06:08.73 Air.in :gulp
@17:06:08.79 Air.seek :topVent
@17:06:15.37 14.7psi after gulp
@17:06:15.64 Reached 14.8 of 15.5psi after pumping 1777uL (1.00 strokes)
@17:06:15.66 Air.in :default
@17:06:15.74 Air.hold
@17:06:16.03 Air.in :pressurize
@17:06:16.09 Air.seek :pressurized
@17:06:22.56 12.2psi after pressurize
@17:06:22.58 Air.in :compressLysate
@17:06:22.65 Air.seek :evac
@17:06:50.33 13.2psi after compress
@17:07:00.91 14.4psi after compress (stable)
@17:07:06.13 Air.in :gulp
@17:07:06.19 Air.seek :topVent
@17:07:12.78 13.6psi after gulp
@17:07:18.39 14.2psi after gulp (stable)
@17:07:18.47 Air.in :pressurize
@17:07:18.55 Air.seek :pressurized
@17:07:25.02 14.3psi after pressurize
@17:07:25.04 Air.in :compressLysate
@17:07:25.11 Air.seek :evac
@17:07:25.43 Reached 14.0 of 15.5psi after pumping 3169uL (1.78 strokes)
@17:07:25.44 Air.in :default
@17:07:25.51 Air.hold
@17:07:25.85 Air.in :compressLysate
@17:07:25.91 Air.seek :evac
@17:07:50.44 Air.in :default
@17:07:50.50 Air.hold
@17:07:50.68 <PROCESSING> Pulled 675uL lysate @14.4psi
@17:07:50.89 Lysis1.seek 800uL,nil
@17:07:51.09 <pump> Air.in :compressLysate
@17:07:51.15 Air.seek :evac
@17:07:53.99 15.1psi after compress
@17:07:54.21 Reached 15.6 of 15.5psi after pumping 1777uL (1.00 strokes)
@17:07:54.24 Air.in :default
@17:07:54.30 Air.hold
@17:07:54.54 Air.in :compressLysate
@17:07:54.60 Air.seek :evac
@17:07:54.82 15.1psi after compress
@17:07:56.56 Air.in :default
@17:07:56.61 Air.hold
@17:07:56.81 <PROCESSING> Air.in :glide
@17:07:56.87 Air.seek :fullVent
@17:07:58.67 Handoff.select :waste
@17:07:58.80 Recovered 800uL lysate @14.0psi
@17:07:58.81 delay 3 seconds
@17:08:01.89 After 3 delay Recovered 800uL lysate @2.5psi
@17:08:02.96 <LRAUV> -> Cmd.stop
@17:08:03.44 <closeHandoff> Handoff.select :close
@17:08:03.59 <LRAUV> Gate.power :heat,:OFF
@17:08:03.61 Gate.power :cartridge,:OFF
@17:08:03.70 PinPort.in :unlocking
@17:08:03.76 PinPort.seek :clear
@17:08:06.19 Carousel.in :default
@17:08:06.35 Carousel.home.seek 48
@17:08:06.66 Plunge.seek :locked
@17:08:08.05 <Archive> Archive.in :unlocking
@17:08:08.17 Archive.seek :clear
@17:08:08.22 <Air> Air.in :unlocking
@17:08:08.35 Air.seek :clear
@17:08:08.48 <Lysis1> Lysis1.in :unlocking
@17:08:08.60 Lysis1.seek :clear
@17:08:08.65 <Lysis2> Lysis2.in :unlocking
@17:08:08.76 Lysis2.seek :clear
@17:08:13.50 <LRAUV> Gate.power :motor,:OFF
@17:08:13.52 Gate.power :analytic1,:OFF
@17:08:13.55 Gate.power :analytic2,:OFF
@17:08:13.57 Gate.power :analytic3,:OFF
@17:08:13.59 Gate.power :core,:OFF
@17:08:13.62 Gate.power :main,:OFF
@17:08:13.65 Safely stopped and ready to power off
##-- file closed 2025-12-06T01:24:43.083Z

##-- file opened 2025-12-06T01:24:43.090Z
@17:24:43.19 -> showStatus
@17:24:45.23 -> Cmd.loadCartridge -15
@17:24:45.32 Gate.power :main,:ON,limit to 5000mA
@17:24:45.33 Gate.power :analytic1,:ON,limit to 4000mA
@17:24:45.36 Gate.power :analytic2,:ON,limit to 4000mA
@17:24:45.38 Gate.power :analytic3,:ON,limit to 4000mA
@17:24:45.43 Powering core bus
@17:24:48.19 Plunge.configure :default
@17:24:48.27 SamplePump.configure :default
@17:24:48.35 Toroid.configure :default
@17:24:48.43 Carousel.configure :default
@17:24:48.50 Abs.configure :default
@17:24:48.58 Archive.configure :default
@17:24:48.66 Air.configure :default
@17:24:48.72 Lysis1.configure :default
@17:24:48.77 Lysis2.configure :default
@17:24:48.85 PinPort.configure :default
@17:24:48.92 WARNING:  Multiple controller firmware versions in use
[:sampler0, :syringe1, :sampler1, :valve0, :ambient0, :valve1, :syringe0]=>4.93, [:carousel0]=>4.94
@17:24:48.94 Gate.power :motor,:ON,limit to 5000mA
@17:24:49.14 <Plunge_home> Plunge.home.jog 0
@17:24:49.68 <PinPort_home> PinPort.home.jog 0
@17:24:49.72 <Lysis2_home> Lysis2.home.jog 0
@17:24:49.75 <Air_home> Air.home.jog 0
@17:24:49.78 <Archive_home> Archive.home.jog 0
@17:24:49.80 <Carousel_home> Carousel.home.jog 0
@17:24:49.84 <Toroid_home> Toroid.home.jog 0
@17:24:49.87 <Lysis1_home> Lysis1.home.jog 0
@17:24:50.29 <Plunge_home> Plunge.in :homing
@17:24:50.56 Plunge.jog 1526
@17:24:50.73 <PinPort_home> PinPort.in :homing
@17:24:50.76 <Lysis1_home> Lysis1.in :homing
@17:24:50.79 <Air_home> Air.in :homing
@17:24:50.81 <Archive_home> Archive.in :homing
@17:24:50.84 <Lysis2_home> Lysis2.in :homing
@17:24:50.87 <Toroid_home> Toroid.in :homing
@17:24:51.08 <PinPort_home> PinPort.jog -1202
@17:24:51.12 <Lysis1_home> Lysis1.jog 304
@17:24:51.15 <Air_home> Air.jog 361
@17:24:51.29 <Toroid_home> Toroid.jog 1304
@17:24:51.32 <Lysis2_home> Lysis2.jog 1610
@17:24:51.36 <Archive_home> Archive.jog 320
@17:24:51.85 <Plunge_home> Plunge.setRawPosition! 50
@17:24:51.93 Plunge.seek :home
@17:24:51.95 <Lysis1_home> Lysis1.setRawPosition! 50
@17:24:52.07 Lysis1.seek :home
@17:24:52.16 <Air_home> Air.setRawPosition! 0
@17:24:52.18 <Archive_home> Archive.setRawPosition! 50
@17:24:52.27 <Air_home> Air.seek :home
@17:24:52.31 <Archive_home> Archive.seek :home
@17:24:52.47 <Air_home> Air.in :default
@17:24:52.50 <Lysis1_home> Lysis1.in :default
@17:24:52.52 <Plunge_home> Plunge.in :default
@17:24:52.67 <Archive_home> Archive.in :default
@17:24:52.76 <Lysis2_home> Lysis2.setRawPosition! 50
@17:24:52.82 Lysis2.seek :home
@17:24:52.94 <PinPort_home> PinPort.setRawPosition! 0
@17:24:52.99 PinPort.seek :home
@17:24:53.10 <Lysis2_home> Lysis2.in :default
@17:24:53.13 <PinPort_home> PinPort.in :default
@17:24:55.75 <Toroid_home> Toroid.setRawPosition! 0
@17:24:55.82 Toroid.seek :home
@17:24:55.89 Toroid.in :default
@17:24:56.37 <clearToroid> Toroid.seek :clear
@17:24:56.50 <LRAUV> PinPort.in :unlocking
@17:24:56.58 PinPort.seek :clear
@17:25:00.01 Air.seek :clear
@17:25:00.92 Lysis1.seek :clear
@17:25:01.71 Lysis2.seek :clear
@17:25:02.50 Archive.seek :clear
@17:25:03.29 Carousel.in :default
@17:25:03.40 Carousel.home.seek 48
@17:25:03.67 Selecting Cartridge 47
@17:25:03.93 Carousel.seek 47
@17:25:04.48 Carousel.reconfigure :align
@17:25:04.59 Lysis1.in :fast
@17:25:04.66 Lysis1.seek :locked
@17:25:07.14 <Plunge> Plunge.seek :locked
@17:25:07.29 <Air> Air.in :fast
@17:25:07.34 Air.seek :locked
@17:25:08.24 <LRAUV> Carousel.reconfigure :locked
@17:25:08.38 Powering cartridge bus
@17:25:11.00 Gate.setIR 1750,5000,0,6
@17:25:11.12 Gate.setIR 0,5000,0,6
@17:25:11.19 PuckHeater.configure :default
@17:25:11.26 Gate.power :heat,:ON,limit to 5000mA
@17:25:11.45 Mounted lysis Cartridge board#J59960
@17:25:11.49 Using default cartridge heat loss curve for HeatingFunctionInCarousel
@17:25:25.41 -> Cmd.startFiltering
@17:25:25.65 <FILTERING> Preparing to filter lyseHoldBS_bac cartridge in slot 47
@17:25:25.90 <sealing> Air.in :default
@17:25:26.03 Air.seek :sealed
@17:25:26.06 <FILTERING> Priming sample loop w/200ml, bypass w/10ml
@17:25:26.12 SamplePump.setPosition! 0ml
@17:25:26.19 Bypass.open
@17:25:27.80 Intake.open
@17:25:28.93 Sea 79.5m deep (132.1psia)
@17:25:28.95 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@17:25:31.56 Vacuum tare is 52% of full scale at 11C (drifted 1%)
@17:25:31.69 <closeIntake> Intake.close
@17:25:31.76 <closeBypass> Bypass.close
@17:25:32.65 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 131.8psia
@17:25:32.67 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@17:25:37.25 Toroid pressure rose 1% to 133.0psia
@17:25:37.27 Bypass.open
@17:25:38.11 Intake.open
@17:25:39.85 Abs.in :default
@17:25:39.91 Abs.hold
@17:25:40.11 SamplePump.in :start
@17:25:40.17 SamplePump.seek 10ml,1:10
@17:25:40.80 <pumpStarted> SamplePump.configure :prime
@17:25:53.83 <FILTERING> Abs.stop
@17:25:53.88 SamplePump.setPosition! 0ml
@17:25:53.93 Exhaust.open
@17:25:55.56 <closeBypass> Bypass.close
@17:25:57.22 <FILTERING> Abs.in :default
@17:25:57.28 Abs.hold
@17:25:57.40 Sea 84.7m deep (139.9psia)
@17:25:57.58 SamplePump.in :start
@17:25:57.65 SamplePump.seek 200ml,13:50
@17:25:58.27 <pumpStarted> SamplePump.configure :prime
@17:30:23.28 <FILTERING> Abs.stop
@17:30:23.36 Bypass.open
@17:30:24.20 Intake.close
@17:30:25.32 Toroid.in :hold
@17:30:25.38 Toroid.seek :crack
@17:30:27.85 Toroid.seek :cartridge
@17:30:29.97 Toroid.in :default
@17:30:30.03 Toroid.seek :clear
@17:30:33.96 <closeExhaust> Exhaust.close
@17:30:34.01 <closeBypass> Bypass.close
@17:30:34.90 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 132.4psia
@17:30:34.92 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@17:30:39.55 Sampling 1000.0ml
@17:30:39.94 SamplePump.setPosition! 0ml
@17:30:40.00 Exhaust.open
@17:30:40.93 Intake.open
@17:30:42.15 Abs.in :default
@17:30:42.21 Abs.hold
@17:30:42.34 Sea 81.3m deep (134.8psia)
@17:30:42.50 SamplePump.in :start
@17:30:42.56 SamplePump.seek 1000ml,1:07:10
@17:30:43.20 <pumpStarted> SamplePump.configure :sample
@17:30:45.58 <sampling> Cartridge 47 filtering 2/1000ml at 0.58ml/s with 22.6psi vacuum 82.7m deep (136.9psia)
@17:31:45.63 Cartridge 47 filtering 35/1000ml at 0.56ml/s with 21.8psi vacuum 85.6m deep (141.2psia)
@17:32:45.63 Cartridge 47 filtering 69/1000ml at 0.55ml/s with 21.5psi vacuum 79.1m deep (131.6psia)
@17:33:45.63 Cartridge 47 filtering 101/1000ml at 0.55ml/s with 16.9psi vacuum 91.9m deep (150.6psia)
@17:34:45.63 Cartridge 47 filtering 134/1000ml at 0.55ml/s with 28.1psi vacuum 78.2m deep (130.3psia)
@17:35:45.63 Cartridge 47 filtering 167/1000ml at 0.55ml/s with 17.5psi vacuum 87.1m deep (143.4psia)
@17:36:45.63 Cartridge 47 filtering 200/1000ml at 0.55ml/s with 21.5psi vacuum 82.1m deep (136.0psia)
@17:37:45.63 Cartridge 47 filtering 233/1000ml at 0.54ml/s with 21.2psi vacuum 80.4m deep (133.5psia)
@17:38:45.63 Cartridge 47 filtering 265/1000ml at 0.54ml/s with 24.6psi vacuum 83.5m deep (138.2psia)
@17:39:45.63 Cartridge 47 filtering 297/1000ml at 0.54ml/s with 19.5psi vacuum 88.8m deep (145.9psia)
@17:40:45.64 Cartridge 47 filtering 329/1000ml at 0.53ml/s with 23.2psi vacuum 80.1m deep (133.1psia)
@17:41:45.63 Cartridge 47 filtering 361/1000ml at 0.53ml/s with 26.5psi vacuum 81.7m deep (135.4psia)
@17:42:45.62 Cartridge 47 filtering 393/1000ml at 0.53ml/s with 22.6psi vacuum 76.6m deep (127.9psia)
@17:43:45.63 Cartridge 47 filtering 425/1000ml at 0.53ml/s with 18.0psi vacuum 94.0m deep (153.7psia)
@17:44:45.63 Cartridge 47 filtering 456/1000ml at 0.52ml/s with 25.8psi vacuum 80.6m deep (133.8psia)
@17:45:45.63 Cartridge 47 filtering 487/1000ml at 0.52ml/s with 29.1psi vacuum 73.3m deep (123.0psia)
@17:46:45.67 Cartridge 47 filtering 519/1000ml at 0.52ml/s with 17.3psi vacuum 90.9m deep (149.0psia)
@17:47:45.63 Cartridge 47 filtering 550/1000ml at 0.52ml/s with 21.5psi vacuum 80.8m deep (134.1psia)
@17:48:45.63 Cartridge 47 filtering 581/1000ml at 0.52ml/s with 23.9psi vacuum 88.7m deep (145.8psia)
@17:49:45.63 Cartridge 47 filtering 611/1000ml at 0.51ml/s with 27.6psi vacuum 78.6m deep (130.8psia)
@17:50:45.63 Cartridge 47 filtering 642/1000ml at 0.51ml/s with 26.1psi vacuum 76.7m deep (128.0psia)
@17:51:45.63 Cartridge 47 filtering 672/1000ml at 0.51ml/s with 28.0psi vacuum 78.7m deep (131.0psia)
@17:52:45.63 Cartridge 47 filtering 703/1000ml at 0.50ml/s with 29.1psi vacuum 71.2m deep (120.0psia)
@17:53:45.63 Cartridge 47 filtering 733/1000ml at 0.50ml/s with 19.2psi vacuum 89.9m deep (147.5psia)
@17:54:45.62 Cartridge 47 filtering 763/1000ml at 0.50ml/s with 20.8psi vacuum 82.3m deep (136.3psia)
@17:55:45.63 Cartridge 47 filtering 792/1000ml at 0.49ml/s with 27.4psi vacuum 79.1m deep (131.7psia)
@17:56:45.63 Cartridge 47 filtering 822/1000ml at 0.49ml/s with 21.8psi vacuum 86.6m deep (142.7psia)
@17:57:45.63 Cartridge 47 filtering 851/1000ml at 0.49ml/s with 25.9psi vacuum 74.4m deep (124.7psia)
@17:58:45.99 Cartridge 47 filtering 880/1000ml at 0.49ml/s with 20.7psi vacuum 89.6m deep (147.1psia)
@17:59:45.64 Cartridge 47 filtering 909/1000ml at 0.48ml/s with 20.4psi vacuum 82.8m deep (137.0psia)
@18:00:45.66 Cartridge 47 filtering 938/1000ml at 0.48ml/s with 17.2psi vacuum 87.9m deep (144.7psia)
@18:01:45.63 Cartridge 47 filtering 966/1000ml at 0.47ml/s with 19.5psi vacuum 88.6m deep (145.6psia)
@18:02:45.64 Cartridge 47 filtering 994/1000ml at 0.47ml/s with 20.2psi vacuum 83.0m deep (137.3psia)
@18:02:57.50 <FILTERING> Abs.stop
@18:02:57.57 <closeIntake> Intake.close
@18:02:57.64 <closeExhaust> Exhaust.close
@18:02:57.81 <FILTERING> Toroid.in :hold
@18:02:57.87 Toroid.seek :bypass
@18:03:01.47 Toroid.in :default
@18:03:01.53 Toroid.seek :clear
@18:03:05.12 Sampled  1000.0ml
@18:03:06.79 <LRAUV> -> Cmd.startProcessing
@18:03:07.14 <PROCESSING> Handoff.select :waste
@18:03:07.27 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@18:03:13.86 Syringe tare is 50% of full scale at 13C (drifted -0%)
@18:03:13.89 PinPort.in :locking
@18:03:13.96 PinPort.seek :locked
@18:03:16.40 <posArchive> Archive.in :fast
@18:03:16.44 <posLyS2> Lysis2.in :fast
@18:03:16.54 <PROCESSING> Lysis1.seek 1250uL
@18:03:16.58 <posArchive> Archive.seek :empty
@18:03:16.62 <posLyS2> Lysis2.seek :empty
@18:03:25.07 <PROCESSING> Evacuating puck with filter bubble point of 50psi
@18:03:25.09 PinPort.seek :locked
@18:03:25.19 Handoff.select :waste
@18:03:25.21 Plunge.seek :puckBottom
@18:03:26.53 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@18:03:34.61 Air syringe pumping up to 1 strokes to reach 8psi
@18:03:34.62 Air stroke #1 of 1
@18:03:34.72 Air.in :pressurize
@18:03:34.78 Air.seek :pressurized
@18:03:39.86 -0.1psi after pressurize
@18:03:39.89 Air.in :compress
@18:03:39.95 Air.seek :evac
@18:03:46.98 1.7psi after compress
@18:03:58.55 -0.0psi after compress (stable)
@18:03:58.56 Air.in :default
@18:03:58.63 Air.hold
@18:03:58.81 at -0.2psi Opened to waste
@18:03:58.82 Handoff.select :blocked
@18:03:58.96 Plunge.seek :puckTop
@18:03:59.86 Air syringe pumping up to 8 strokes to reach 50psi
@18:03:59.87 Air stroke #1 of 8
@18:03:59.98 Air.in :compress
@18:04:00.04 Air.seek :evac
@18:04:00.17 1.1psi after compress
@18:04:10.75 1.8psi after compress (stable)
@18:04:10.77 Air.in :gulp
@18:04:10.85 Air.seek :topVent
@18:04:17.44 1.7psi after gulp
@18:04:23.02 1.9psi after gulp (stable)
@18:04:23.03 Air stroke #2 of 8
@18:04:23.12 Air.in :pressurize
@18:04:23.18 Air.seek :pressurized
@18:04:29.61 1.7psi after pressurize
@18:04:29.64 Air.in :compress
@18:04:29.71 Air.seek :evac
@18:04:36.74 16.7psi after compress
@18:04:48.31 20.4psi after compress (stable)
@18:04:48.33 Air.in :gulp
@18:04:48.40 Air.seek :topVent
@18:04:54.99 20.0psi after gulp
@18:05:00.56 20.3psi after gulp (stable)
@18:05:00.57 Air stroke #3 of 8
@18:05:00.67 Air.in :pressurize
@18:05:00.73 Air.seek :pressurized
@18:05:07.16 20.0psi after pressurize
@18:05:07.17 Air.in :compress
@18:05:07.25 Air.seek :evac
@18:05:14.27 31.6psi after compress
@18:05:26.34 35.5psi after compress (stable)
@18:05:26.36 Air.in :gulp
@18:05:26.43 Air.seek :topVent
@18:05:33.02 35.7psi after gulp
@18:05:38.59 36.0psi after gulp (stable)
@18:05:38.61 Air stroke #4 of 8
@18:05:38.70 Air.in :pressurize
@18:05:38.76 Air.seek :pressurized
@18:05:45.18 35.4psi after pressurize
@18:05:45.20 Air.in :compress
@18:05:45.26 Air.seek :evac
@18:05:52.28 44.0psi after compress
@18:06:04.86 48.2psi after compress (stable)
@18:06:04.88 Air.in :gulp
@18:06:04.95 Air.seek :topVent
@18:06:11.53 48.4psi after gulp
@18:06:17.11 48.5psi after gulp (stable)
@18:06:17.12 Air stroke #5 of 8
@18:06:17.21 Air.in :pressurize
@18:06:17.27 Air.seek :pressurized
@18:06:23.73 48.1psi after pressurize
@18:06:23.75 Air.in :compress
@18:06:23.82 Air.seek :evac
@18:06:30.29 Reached 50.7 of 50.0psi after pumping 8851uL (4.98 strokes)
@18:06:30.31 Air.in :default
@18:06:30.37 Air.hold
@18:06:30.45 Plunge.seek :puckBottom
@18:06:31.34 delay 3 seconds
@18:06:34.36 Handoff.select :waste
@18:06:34.49 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@18:06:52.07 delay 1:00
@18:07:52.09 Handoff.select :blocked
@18:07:52.21 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@18:08:14.79 Handoff.select :waste
@18:08:14.91 1.0psi at evacEnd
@18:08:14.92 Handoff.select :blocked
@18:08:15.05 Plunge.seek :puckTop
@18:08:15.94 Air.in :fast
@18:08:16.01 Air.seek :topVent
@18:08:22.54 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi
@18:08:29.11 Stable @12.8psi after Handoff blocked
@18:08:29.13 Air syringe pumping up to 8 strokes to reach 35psi
@18:08:29.15 Air stroke #1 of 8
@18:08:29.24 Air.in :pressurize
@18:08:29.30 Air.seek :pressurized
@18:08:35.73 12.4psi after pressurize
@18:08:35.76 Air.in :compress
@18:08:35.83 Air.seek :evac
@18:08:42.85 21.6psi after compress
@18:08:55.42 24.8psi after compress (stable)
@18:08:55.44 Air.in :gulp
@18:08:55.51 Air.seek :topVent
@18:09:02.10 24.9psi after gulp
@18:09:07.67 25.0psi after gulp (stable)
@18:09:07.68 Air stroke #2 of 8
@18:09:07.78 Air.in :pressurize
@18:09:07.84 Air.seek :pressurized
@18:09:14.27 24.8psi after pressurize
@18:09:14.29 Air.in :compress
@18:09:14.36 Air.seek :evac
@18:09:21.39 32.2psi after compress
@18:09:25.62 Reached 35.1 of 35.0psi after pumping 3553uL (2.00 strokes)
@18:09:25.63 Air.in :default
@18:09:25.69 Air.hold
@18:09:25.76 Plunge.seek :puckBottom
@18:09:26.67 delay 3 seconds
@18:09:29.68 Handoff.select :waste
@18:09:29.81 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@18:09:36.38 delay 1:00
@18:10:36.41 Handoff.select :blocked
@18:10:36.53 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@18:10:59.10 Handoff.select :waste
@18:10:59.23 0.9psi at evacEnd
@18:10:59.25 Plunge.seek :pTop
@18:11:00.15 delay 5 seconds
@18:11:05.17 Air.in :glide
@18:11:05.22 Air.seek :fullVent
@18:11:07.03 delay 2 seconds
@18:11:09.06 Plunge.seek :pBottom
@18:11:09.94 Seawater evacuated, Pressure above/below filter released.
@18:11:09.96 Plunge.seek :locked
@18:11:11.27 Handoff.select :waste
@18:11:11.36 Lysis1.in :slow
@18:11:11.42 Lysis1.seek 950uL
@18:11:25.58 delay 5 seconds
@18:11:30.60 Handoff.select :blocked
@18:11:30.73 Plunge.seek :puckTop
@18:11:31.66 Lysis1.seek 350uL
@18:11:59.82 delay 3 seconds
@18:12:02.90 Air.in mix with 2.32937 parts Lysis1
@18:12:03.47 <Airmixer> Air.seek :evac
@18:12:03.53 <PROCESSING> Lysis1.seek 190uL
@18:12:11.30 Air.in :fast
@18:12:11.36 Air.seek :full
@18:12:18.00 Lysis1.seek :empty
@18:12:27.13 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @25.2psi
@18:12:27.15 Plunge.seek :puckBottom
@18:12:28.15 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @19.6psi
@18:12:28.16 Polling Can every 2:00
@18:12:28.71 PuckHeater.configure :default #ambient 13.0C
@18:12:28.77 PuckHeater.seek 71.6C
@18:14:04.17 PuckHeater.hold 2:00,71.6C
@18:16:04.12 Polling Can every 2:00
@18:16:04.66 PuckHeater.configure :default #ambient 13.0C
@18:16:04.72 PuckHeater.seek 70.6C
@18:16:04.86 PuckHeater.hold 2:00,70.6C
@18:18:04.79 Polling Can every 2:00
@18:18:05.33 PuckHeater.configure :default #ambient 12.9C
@18:18:05.39 PuckHeater.hold 18:00,69.5C
@18:36:03.38 Polling Can every 2:00
@18:36:03.92 PuckHeater.configure :default #ambient 12.8C
@18:36:03.98 PuckHeater.seek 105.4C
@18:37:12.86 PuckHeater.hold 2:00,105.4C
@18:39:12.69 Polling Can every 2:00
@18:39:13.23 PuckHeater.configure :default #ambient 12.9C
@18:39:13.29 PuckHeater.seek 104.4C
@18:39:13.43 PuckHeater.hold 2:00,104.4C
@18:41:13.28 Polling Can every 2:00
@18:41:13.82 PuckHeater.configure :default #ambient 12.9C
@18:41:13.88 PuckHeater.hold 2:00,103.3C
@18:43:13.77 delay 5:00
@18:48:13.83 Lysate cooked @23.9psi
@18:48:13.85 Plunge.seek :puckTop
@18:48:14.75 Air syringe pumping up to 5 strokes to reach 40psi
@18:48:14.76 Air stroke #1 of 5
@18:48:14.86 Air.in :pressurize
@18:48:14.92 Air.seek :pressurized
@18:48:21.36 19.8psi after pressurize
@18:48:21.38 Air.in :compress
@18:48:21.45 Air.seek :evac
@18:48:27.29 Reached 41.2 of 40.0psi after pumping 1709uL (0.96 strokes)
@18:48:27.31 Air.in :default
@18:48:27.37 Air.hold
@18:48:27.45 delay 2 seconds
@18:48:29.48 Air.in :fast
@18:48:29.53 Air.seek :full
@18:48:35.92 Plunge.seek :puckBottom
@18:48:36.87 Lysis1.seek 138uL
@18:48:43.47 delay 2 seconds
@18:48:45.49 Plunge.seek :puckTop
@18:48:46.39 Lysis1.seek :empty
@18:48:53.00 Plunge.seek :puckBottom
@18:48:54.06 Lysis1.in :extract
@18:48:54.15 Lysis1.seek 675uL,nil
@18:48:56.50 <pump> Air.in :pressurize
@18:48:56.56 Air.seek :pressurized
@18:49:03.08 12.5psi after pressurize
@18:49:03.11 Air.in :compressLysate
@18:49:03.20 Air.seek :evac
@18:49:19.38 Reached 15.7 of 15.5psi after pumping 1615uL (0.91 strokes)
@18:49:19.39 Air.in :default
@18:49:19.46 Air.hold
@18:49:20.31 Air.in :compressLysate
@18:49:20.36 Air.seek :evac
@18:49:32.45 14.6psi after compress
@18:49:32.69 Reached 14.8 of 15.5psi after pumping 1777uL (1.00 strokes)
@18:49:32.73 Air.in :default
@18:49:32.83 Air.hold
@18:49:33.13 Air.in :compressLysate
@18:49:33.19 Air.seek :evac
@18:49:33.36 13.5psi after compress
@18:49:43.94 12.4psi after compress (stable)
@18:49:49.11 Air.in :gulp
@18:49:49.17 Air.seek :topVent
@18:49:55.76 12.9psi after gulp
@18:50:01.85 13.3psi after gulp (stable)
@18:50:01.96 Air.in :pressurize
@18:50:02.03 Air.seek :pressurized
@18:50:08.52 12.1psi after pressurize
@18:50:08.54 Air.in :compressLysate
@18:50:08.61 Air.seek :evac
@18:50:36.35 13.8psi after compress
@18:50:36.88 Air.in :default
@18:50:36.93 Air.hold
@18:50:37.11 <PROCESSING> Pulled 675uL lysate @13.2psi
@18:50:37.28 Lysis1.seek 800uL,nil
@18:50:37.46 <pump> Air.in :compressLysate
@18:50:37.53 Air.seek :evac
@18:50:37.70 13.1psi after compress
@18:50:48.28 13.7psi after compress (stable)
@18:50:53.42 Air.in :default
@18:50:53.56 Air.hold
@18:50:53.63 <PROCESSING> Lysis1.in :default
@18:50:53.71 Lysis1.hold
@18:50:53.96 Air.in :glide
@18:50:54.02 Air.seek :fullVent
@18:50:55.82 Handoff.select :waste
@18:50:55.94 Recovered 675uL lysate @14.1psi
@18:50:55.96 delay 3 seconds
@18:50:59.04 After 3 delay Recovered 675uL lysate @1.5psi
@18:51:00.22 <LRAUV> -> Cmd.stop
@18:51:00.32 <closeHandoff> Handoff.select :close
@18:51:00.47 <LRAUV> Gate.power :heat,:OFF
@18:51:00.48 Gate.power :cartridge,:OFF
@18:51:00.58 PinPort.in :unlocking
@18:51:00.64 PinPort.seek :clear
@18:51:03.07 Carousel.in :default
@18:51:03.18 Carousel.home.seek 47
@18:51:03.49 Plunge.seek :locked
@18:51:04.86 <Archive> Archive.in :unlocking
@18:51:04.99 Archive.seek :clear
@18:51:05.03 <Air> Air.in :unlocking
@18:51:05.19 Air.seek :clear
@18:51:05.25 <Lysis1> Lysis1.in :unlocking
@18:51:05.40 Lysis1.seek :clear
@18:51:05.46 <Lysis2> Lysis2.in :unlocking
@18:51:05.55 Lysis2.seek :clear
@18:51:10.31 <LRAUV> Gate.power :motor,:OFF
@18:51:10.34 Gate.power :analytic1,:OFF
@18:51:10.37 Gate.power :analytic2,:OFF
@18:51:10.39 Gate.power :analytic3,:OFF
@18:51:10.41 Gate.power :core,:OFF
@18:51:10.44 Gate.power :main,:OFF
@18:51:10.46 Safely stopped and ready to power off
##-- file closed 2025-12-06T03:04:55.335Z

##-- file opened 2025-12-06T03:04:55.337Z
@19:04:55.58 -> Cmd.stop
@19:04:55.62 ready to power off
##-- file closed 2025-12-06T08:54:16.985Z