##-- file opened 2025-09-14T08:28:21.235Z @01:28:22.07 -> showStatus @01:28:24.10 -> Cmd.loadCartridge -1 @01:28:24.18 Gate.power :main,:ON,limit to 5000mA @01:28:24.20 Gate.power :analytic1,:ON,limit to 4000mA @01:28:24.22 Gate.power :analytic2,:ON,limit to 4000mA @01:28:24.25 Gate.power :analytic3,:ON,limit to 4000mA @01:28:24.31 Powering core bus @01:28:27.01 Plunge.configure :default @01:28:27.09 SamplePump.configure :default @01:28:27.17 Toroid.configure :default @01:28:27.24 Carousel.configure :default @01:28:27.32 Abs.configure :default @01:28:27.40 Archive.configure :default @01:28:27.48 Air.configure :default @01:28:27.54 Lysis1.configure :default @01:28:27.60 Lysis2.configure :default @01:28:27.67 PinPort.configure :default @01:28:27.74 All elf controllers running firmware version 4.93 @01:28:27.76 Gate.power :motor,:ON,limit to 5000mA @01:28:27.97 Plunge.home.jog 0 @01:28:28.52 PinPort.home.jog 0 @01:28:28.56 Lysis2.home.jog 0 @01:28:28.58 Lysis1.home.jog 0 @01:28:28.61 Archive.home.jog 0 @01:28:28.64 Carousel.home.jog 0 @01:28:28.67 Toroid.home.jog 0 @01:28:28.70 Air.home.jog 0 @01:28:29.31 Plunge.in :homing @01:28:29.49 Plunge.jog 1582 @01:28:29.54 Lysis2.in :homing @01:28:29.57 Lysis1.in :homing @01:28:29.59 Air.in :homing @01:28:29.63 Toroid.in :homing @01:28:29.66 Archive.in :homing @01:28:29.70 PinPort.in :homing @01:28:29.93 Lysis2.jog 1609 @01:28:29.98 Air.jog 366 @01:28:30.01 Lysis1.jog 308 @01:28:30.04 Archive.jog 1596 @01:28:30.07 Toroid.jog 1306 @01:28:30.13 PinPort.jog -1195 @01:28:30.79 Air.setRawPosition! 0 @01:28:30.82 Lysis1.setRawPosition! 50 @01:28:30.92 Air.seek :home @01:28:31.30 Lysis1.seek :home @01:28:31.49 Lysis2.setRawPosition! 50 @01:28:31.52 Air.in :default @01:28:31.54 Plunge.setRawPosition! 50 @01:28:31.67 Lysis2.seek :home @01:28:31.70 Plunge.seek :home @01:28:31.87 Archive.setRawPosition! 50 @01:28:31.90 Lysis1.in :default @01:28:32.03 Archive.seek :home @01:28:32.13 PinPort.setRawPosition! 0 @01:28:32.17 Lysis2.in :default @01:28:32.19 Plunge.in :default @01:28:32.32 PinPort.seek :home @01:28:32.45 Archive.in :default @01:28:32.47 PinPort.in :default @01:28:34.58 Toroid.setRawPosition! 0 @01:28:34.64 Toroid.seek :home @01:28:34.71 Toroid.in :default @01:28:34.78 Toroid.seek :clear @01:28:34.90 PinPort.in :unlocking @01:28:34.99 PinPort.seek :clear @01:28:38.44 Air.seek :clear @01:28:39.33 Lysis1.seek :clear @01:28:40.12 Lysis2.seek :clear @01:28:40.91 Archive.seek :clear @01:28:41.69 Carousel.in :default @01:28:41.80 Carousel.home.seek 21 @01:28:42.07 Selecting Cartridge 20 @01:28:42.33 Carousel.seek 20 @01:28:42.72 Slide::Error in LRAUV -- Storage Carousel (SC) positionErr at 20 + 15 counts (sensed 20 + 21 counts) @01:28:42.74 Retry #1 of 4 @01:28:42.76 Carousel.hold @01:28:42.88 Carousel.in :default @01:28:42.94 Carousel.home.seek 20 @01:28:43.29 Lysis1.in :fast @01:28:43.35 Lysis1.seek :locked @01:28:45.84 Plunge.seek :locked @01:28:45.97 Air.in :fast @01:28:46.04 Air.seek :locked @01:28:46.97 Carousel.reconfigure :locked @01:28:47.10 Powering cartridge bus @01:28:49.74 Gate.power :cartridge,:OFF @01:28:50.29 Mounted archive Cartridge @01:29:03.84 -> Cmd.startFiltering @01:29:04.08 Preparing to filter aRNAl_bac cartridge in slot 20 @01:29:04.36 Priming sample loop w/200ml, bypass w/10ml @01:29:04.37 SamplePump.setPosition! 0ml @01:29:04.43 Bypass.open @01:29:06.18 Intake.open @01:29:07.52 Sea 4.3m deep (21.1psia) @01:29:07.54 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @01:29:10.15 Vacuum tare is 50% of full scale at 12C (drifted -1%) @01:29:10.28 Abs.in :default @01:29:10.33 Abs.hold @01:29:10.53 SamplePump.in :start @01:29:10.60 SamplePump.seek 10ml,1:10 @01:29:11.22 SamplePump.configure :prime @01:29:23.40 Abs.stop @01:29:23.46 SamplePump.setPosition! 0ml @01:29:23.52 Exhaust.open @01:29:25.10 Bypass.close @01:29:26.80 Abs.in :default @01:29:26.86 Abs.hold @01:29:26.99 Sea 8.8m deep (27.7psia) @01:29:27.14 SamplePump.in :start @01:29:27.21 SamplePump.seek 200ml,13:50 @01:29:27.84 SamplePump.configure :prime @01:33:35.87 Abs.stop @01:33:35.94 Bypass.open @01:33:36.92 Intake.close @01:33:37.97 Toroid.in :hold @01:33:38.03 Toroid.seek :crack @01:33:40.50 Toroid.seek :cartridge @01:33:42.62 Toroid.in :default @01:33:42.73 Toroid.seek :clear @01:33:46.66 Pressurizing cartridge to 26psi above 17.8psia @01:33:46.68 SamplePump.setPosition! 0ml @01:33:46.73 Intake.open @01:33:47.66 Bypass.close @01:33:48.52 Exhaust.close @01:33:49.31 Abs.in :default @01:33:49.36 Abs.hold @01:33:49.56 SamplePump.in :charge @01:33:49.62 SamplePump.seek 2.3ml,40 seconds @01:33:57.47 Abs.stop @01:33:57.52 Intake.close @01:33:58.91 Pressurized cartridge with 0.267ml to 26.9psi @01:33:58.93 Isolated @42.2psia (24.5psi) @01:33:58.96 Verifying cartridge holds at least 0.65..0.85 of 42.2psia @01:33:59.00 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @01:34:03.58 Cartridge pressure fell 5% to 40.3psia @01:34:03.71 Sampling 1000.0ml @01:34:03.73 SamplePump.setPosition! 0ml @01:34:03.80 Exhaust.open @01:34:04.69 Intake.open @01:34:05.74 Abs.in :default @01:34:05.80 Abs.hold @01:34:05.94 Sea 4.0m deep (20.6psia) @01:34:06.11 SamplePump.in :start @01:34:06.17 SamplePump.seek 1000ml,1:07:10 @01:34:06.80 SamplePump.configure :sample @01:34:09.19 Cartridge 20 filtering 1/1000ml at 0.61ml/s with 23.2psi vacuum 8.2m deep (26.8psia) @01:35:09.24 Cartridge 20 filtering 31/1000ml at 0.49ml/s with 21.3psi vacuum 4.1m deep (20.7psia) @01:36:09.24 Cartridge 20 filtering 59/1000ml at 0.47ml/s with 18.9psi vacuum @01:37:09.24 Cartridge 20 filtering 86/1000ml at 0.45ml/s with 23.3psi vacuum @01:38:09.24 Cartridge 20 filtering 111/1000ml at 0.42ml/s with 19.1psi vacuum 9.7m deep (29.0psia) @01:39:09.24 Cartridge 20 filtering 135/1000ml at 0.40ml/s with 26.3psi vacuum @01:40:09.24 Cartridge 20 filtering 158/1000ml at 0.38ml/s with 23.0psi vacuum 8.2m deep (26.9psia) @01:41:09.24 Cartridge 20 filtering 179/1000ml at 0.36ml/s with 15.1psi vacuum 16.7m deep (39.3psia) @01:42:09.24 Cartridge 20 filtering 200/1000ml at 0.34ml/s with 29.1psi vacuum @01:43:09.24 Cartridge 20 filtering 219/1000ml at 0.32ml/s with 17.1psi vacuum 10.7m deep (30.5psia) @01:44:09.24 Cartridge 20 filtering 237/1000ml at 0.31ml/s with 18.6psi vacuum 6.5m deep (24.3psia) @01:45:09.24 Cartridge 20 filtering 255/1000ml at 0.29ml/s with 25.5psi vacuum @01:46:09.24 Cartridge 20 filtering 271/1000ml at 0.27ml/s with 25.8psi vacuum @01:47:09.24 Cartridge 20 filtering 287/1000ml at 0.26ml/s with 26.9psi vacuum @01:48:09.24 Cartridge 20 filtering 301/1000ml at 0.23ml/s with 22.6psi vacuum @01:48:09.34 SamplePump.stop @01:48:09.40 Intake.close @01:48:09.47 Exhaust.close @01:48:09.66 Toroid.in :hold @01:48:09.72 Toroid.seek :bypass @01:48:13.32 Toroid.in :default @01:48:13.39 Toroid.seek :clear @01:48:16.98 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @01:48:17.00 Abs.stop @01:48:17.52 Sampled 300.6ml @01:48:19.40 -> Cmd.startProcessing @01:48:19.83 Plunge.seek :pTop @01:48:20.65 Sample loop closed. Ready to Deliver Reagents @01:48:20.67 Air.in :default @01:48:20.74 Air.seek :sealed @01:48:23.95 Air.in :slow @01:48:24.01 Air.seek :arEvac @01:49:37.96 delay 1:00 @01:50:37.99 Air.in :default @01:50:38.04 Air.seek :clear @01:50:56.57 Sample preserved. Puck Full. @01:50:57.66 -> Cmd.stop @01:50:57.82 Carousel.in :default @01:50:57.93 Carousel.home.seek 20 @01:50:58.24 Plunge.seek :locked @01:50:59.24 Lysis1.in :unlocking @01:50:59.41 Lysis1.seek :clear @01:51:00.81 Gate.power :motor,:OFF @01:51:00.83 Gate.power :analytic1,:OFF @01:51:00.86 Gate.power :analytic2,:OFF @01:51:00.88 Gate.power :analytic3,:OFF @01:51:00.91 Gate.power :core,:OFF @01:51:00.93 Gate.power :main,:OFF @01:51:00.96 Safely stopped and ready to power off ##-- file closed 2025-09-14T20:26:37.694Z ##-- file opened 2025-09-14T20:26:37.697Z @13:26:38.00 -> showStatus @13:26:40.02 -> Cmd.loadCartridge -1 @13:26:40.11 Gate.power :main,:ON,limit to 5000mA @13:26:40.12 Gate.power :analytic1,:ON,limit to 4000mA @13:26:40.15 Gate.power :analytic2,:ON,limit to 4000mA @13:26:40.17 Gate.power :analytic3,:ON,limit to 4000mA @13:26:40.22 Powering core bus @13:26:42.93 Plunge.configure :default @13:26:43.01 SamplePump.configure :default @13:26:43.10 Toroid.configure :default @13:26:43.18 Carousel.configure :default @13:26:43.26 Abs.configure :default @13:26:43.34 Archive.configure :default @13:26:43.42 Air.configure :default @13:26:43.48 Lysis1.configure :default @13:26:43.54 Lysis2.configure :default @13:26:43.61 PinPort.configure :default @13:26:43.71 All elf controllers running firmware version 4.93 @13:26:43.73 Gate.power :motor,:ON,limit to 5000mA @13:26:43.95 Plunge.home.jog 0 @13:26:44.46 PinPort.home.jog 0 @13:26:44.51 Lysis2.home.jog 0 @13:26:44.53 Lysis1.home.jog 0 @13:26:44.56 Archive.home.jog 0 @13:26:44.59 Carousel.home.jog 0 @13:26:44.61 Toroid.home.jog 0 @13:26:44.65 Air.home.jog 0 @13:26:45.23 Plunge.in :homing @13:26:45.48 Plunge.jog 1585 @13:26:45.53 Lysis2.in :homing @13:26:45.55 Lysis1.in :homing @13:26:45.59 Air.in :homing @13:26:45.62 Toroid.in :homing @13:26:45.65 Archive.in :homing @13:26:45.68 PinPort.in :homing @13:26:45.92 Lysis1.jog 312 @13:26:45.95 Lysis2.jog 1610 @13:26:45.98 Air.jog 365 @13:26:46.01 Archive.jog 1590 @13:26:46.04 Toroid.jog 1304 @13:26:46.10 PinPort.jog -1190 @13:26:46.70 Lysis1.setRawPosition! 50 @13:26:47.13 Air.setRawPosition! 0 @13:26:47.17 Plunge.setRawPosition! 50 @13:26:47.19 Lysis1.seek :home @13:26:47.34 Air.seek :home @13:26:47.45 Plunge.seek :home @13:26:47.58 Lysis2.setRawPosition! 50 @13:26:47.62 Air.in :default @13:26:47.66 Archive.setRawPosition! 50 @13:26:47.70 Lysis1.in :default @13:26:47.75 Lysis2.seek :home @13:26:47.94 Archive.seek :home @13:26:48.02 PinPort.setRawPosition! 0 @13:26:48.09 Plunge.in :default @13:26:48.14 PinPort.seek :home @13:26:48.29 Lysis2.in :default @13:26:48.32 Archive.in :default @13:26:48.44 PinPort.in :default @13:26:50.56 Toroid.setRawPosition! 0 @13:26:50.62 Toroid.seek :home @13:26:50.70 Toroid.in :default @13:26:50.77 Toroid.seek :clear @13:26:50.91 PinPort.in :unlocking @13:26:50.97 PinPort.seek :clear @13:26:54.42 Air.seek :clear @13:26:55.32 Lysis1.seek :clear @13:26:56.12 Lysis2.seek :clear @13:26:56.90 Archive.seek :clear @13:26:57.68 Carousel.in :default @13:26:57.79 Carousel.home.seek 20 @13:26:58.06 Selecting Cartridge 19 @13:26:58.31 Carousel.seek 19 @13:26:58.85 Lysis1.in :fast @13:26:58.92 Lysis1.seek :locked @13:27:01.41 Plunge.seek :locked @13:27:01.55 Air.in :fast @13:27:01.61 Air.seek :locked @13:27:02.53 Carousel.reconfigure :locked @13:27:02.69 Powering cartridge bus @13:27:05.33 Gate.power :cartridge,:OFF @13:27:05.88 Mounted archive Cartridge @13:27:17.82 -> Cmd.startFiltering @13:27:18.08 Preparing to filter aRNAl_bac cartridge in slot 19 @13:27:18.36 Priming sample loop w/200ml, bypass w/10ml @13:27:18.37 SamplePump.setPosition! 0ml @13:27:18.42 Bypass.open @13:27:20.16 Intake.open @13:27:21.14 Sea 6.2m deep (23.9psia) @13:27:21.17 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:27:23.76 Vacuum tare is 50% of full scale at 13C (drifted -1%) @13:27:23.90 Abs.in :default @13:27:23.96 Abs.hold @13:27:24.15 SamplePump.in :start @13:27:24.22 SamplePump.seek 10ml,1:10 @13:27:24.85 SamplePump.configure :prime @13:27:37.03 Abs.stop @13:27:37.08 SamplePump.setPosition! 0ml @13:27:37.15 Exhaust.open @13:27:38.75 Bypass.close @13:27:40.41 Abs.in :default @13:27:40.47 Abs.hold @13:27:40.58 Sea 4.8m deep (21.8psia) @13:27:40.80 SamplePump.in :start @13:27:40.86 SamplePump.seek 200ml,13:50 @13:27:41.49 SamplePump.configure :prime @13:31:49.53 Abs.stop @13:31:49.61 Bypass.open @13:31:50.59 Intake.close @13:31:51.63 Toroid.in :hold @13:31:51.69 Toroid.seek :crack @13:31:54.16 Toroid.seek :cartridge @13:31:56.28 Toroid.in :default @13:31:56.34 Toroid.seek :clear @13:32:00.27 Pressurizing cartridge to 26psi above 18.1psia @13:32:00.28 SamplePump.setPosition! 0ml @13:32:00.34 Intake.open @13:32:01.18 Bypass.close @13:32:02.04 Exhaust.close @13:32:02.92 Abs.in :default @13:32:02.97 Abs.hold @13:32:03.15 SamplePump.in :charge @13:32:03.22 SamplePump.seek 2.3ml,40 seconds @13:32:11.16 Abs.stop @13:32:11.22 Intake.close @13:32:12.22 Pressurized cartridge with 0.274ml to 26.1psi @13:32:12.23 Isolated @41.3psia (23.2psi) @13:32:12.27 Verifying cartridge holds at least 0.65..0.85 of 41.3psia @13:32:12.30 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:32:16.88 Cartridge pressure fell 3% to 40.0psia @13:32:16.97 Sampling 1000.0ml @13:32:16.98 SamplePump.setPosition! 0ml @13:32:17.04 Exhaust.open @13:32:17.91 Intake.open @13:32:18.96 Abs.in :default @13:32:19.02 Abs.hold @13:32:19.28 SamplePump.in :start @13:32:19.35 SamplePump.seek 1000ml,1:07:10 @13:32:19.97 SamplePump.configure :sample @13:32:22.35 Cartridge 19 filtering 1/1000ml at 0.58ml/s with 24.7psi vacuum @13:33:22.41 Cartridge 19 filtering 29/1000ml at 0.47ml/s with 24.4psi vacuum @13:34:22.41 Cartridge 19 filtering 55/1000ml at 0.43ml/s with 24.4psi vacuum 6.4m deep (24.1psia) @13:35:22.41 Cartridge 19 filtering 79/1000ml at 0.40ml/s with 18.9psi vacuum 7.5m deep (25.7psia) @13:36:22.41 Cartridge 19 filtering 102/1000ml at 0.38ml/s with 28.6psi vacuum @13:37:22.41 Cartridge 19 filtering 123/1000ml at 0.34ml/s with 24.7psi vacuum 8.7m deep (27.5psia) @13:38:22.41 Cartridge 19 filtering 142/1000ml at 0.32ml/s with 26.9psi vacuum @13:39:22.41 Cartridge 19 filtering 160/1000ml at 0.31ml/s with 26.9psi vacuum @13:40:22.40 Cartridge 19 filtering 178/1000ml at 0.29ml/s with 28.6psi vacuum @13:41:22.41 Cartridge 19 filtering 194/1000ml at 0.27ml/s with 23.1psi vacuum @13:42:22.41 Cartridge 19 filtering 209/1000ml at 0.26ml/s with 24.2psi vacuum @13:43:22.41 Cartridge 19 filtering 224/1000ml at 0.24ml/s with 20.3psi vacuum 8.9m deep (27.9psia) @13:43:22.51 SamplePump.stop @13:43:22.58 Intake.close @13:43:22.66 Exhaust.close @13:43:22.82 Toroid.in :hold @13:43:22.88 Toroid.seek :bypass @13:43:26.48 Toroid.in :default @13:43:26.55 Toroid.seek :clear @13:43:30.13 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @13:43:30.16 Abs.stop @13:43:30.30 Sampled 223.7ml @13:43:32.34 -> Cmd.startProcessing @13:43:32.82 Plunge.seek :pTop @13:43:33.64 Sample loop closed. Ready to Deliver Reagents @13:43:33.66 Air.in :default @13:43:34.11 Air.seek :sealed @13:43:37.31 Air.in :slow @13:43:37.38 Air.seek :arEvac @13:44:51.30 delay 1:00 @13:45:51.33 Air.in :default @13:45:51.39 Air.seek :clear @13:46:09.91 Sample preserved. Puck Full. @13:46:10.85 -> Cmd.stop @13:46:11.00 Carousel.in :default @13:46:11.11 Carousel.home.seek 19 @13:46:11.41 Plunge.seek :locked @13:46:12.42 Lysis1.in :unlocking @13:46:12.56 Lysis1.seek :clear @13:46:13.96 Gate.power :motor,:OFF @13:46:13.98 Gate.power :analytic1,:OFF @13:46:14.00 Gate.power :analytic2,:OFF @13:46:14.02 Gate.power :analytic3,:OFF @13:46:14.05 Gate.power :core,:OFF @13:46:14.07 Gate.power :main,:OFF @13:46:14.09 Safely stopped and ready to power off ##-- file closed 2025-09-15T01:09:57.049Z