##-- file opened 2025-07-19T05:35:42.468Z
@22:35:42.96 -> showStatus
@22:35:44.56 -> Cmd.loadCartridge -15
@22:35:44.64 Gate.power :main,:ON,limit to 5000mA
@22:35:44.66 Gate.power :analytic1,:ON,limit to 4000mA
@22:35:44.69 Gate.power :analytic2,:ON,limit to 4000mA
@22:35:44.72 Gate.power :analytic3,:ON,limit to 4000mA
@22:35:44.77 Powering core bus
@22:35:47.48 Plunge.configure :default
@22:35:47.56 SamplePump.configure :default
@22:35:47.64 Toroid.configure :default
@22:35:47.72 Carousel.configure :default
@22:35:47.80 Abs.configure :default
@22:35:47.88 Archive.configure :default
@22:35:47.96 Air.configure :default
@22:35:48.02 Lysis1.configure :default
@22:35:48.08 Lysis2.configure :default
@22:35:48.16 PinPort.configure :default
@22:35:48.22 All elf controllers running firmware version 4.92
@22:35:48.25 Gate.power :motor,:ON,limit to 5000mA
@22:35:48.56 <Toroid_home> Toroid.home.jog 0
@22:35:48.62 <Plunge_home> Plunge.home.jog 0
@22:35:49.10 <Lysis2_home> Lysis2.home.jog 0
@22:35:49.14 <Lysis1_home> Lysis1.home.jog 0
@22:35:49.19 <Air_home> Air.home.jog 0
@22:35:49.22 <Archive_home> Archive.home.jog 0
@22:35:49.25 <Carousel_home> Carousel.home.jog 0
@22:35:49.33 <PinPort_home> PinPort.home.jog 0
@22:35:50.19 <Toroid_home> Toroid.in :homing
@22:35:50.29 <Plunge_home> Plunge.in :homing
@22:35:50.49 <Lysis2_home> Lysis2.in :homing
@22:35:50.53 <Air_home> Air.in :homing
@22:35:50.56 <Archive_home> Archive.in :homing
@22:35:50.59 <Toroid_home> Toroid.jog 1279
@22:35:50.62 <Plunge_home> Plunge.jog 1584
@22:35:50.65 <PinPort_home> PinPort.in :homing
@22:35:50.69 <Lysis1_home> Lysis1.in :homing
@22:35:50.87 <Lysis2_home> Lysis2.jog 1608
@22:35:50.97 <Air_home> Air.jog 360
@22:35:51.04 <PinPort_home> PinPort.jog -1191
@22:35:51.06 <Archive_home> Archive.jog 1586
@22:35:51.12 <Lysis1_home> Lysis1.jog 306
@22:35:51.76 <Air_home> Air.setRawPosition! 0
@22:35:51.82 Air.seek :home
@22:35:51.91 Air.in :default
@22:35:51.94 <Lysis1_home> Lysis1.setRawPosition! 50
@22:35:52.03 Lysis1.seek :home
@22:35:52.16 <Plunge_home> Plunge.setRawPosition! 50
@22:35:52.21 Plunge.seek :home
@22:35:52.30 <Lysis1_home> Lysis1.in :default
@22:35:52.40 <Lysis2_home> Lysis2.setRawPosition! 50
@22:35:52.45 Lysis2.seek :home
@22:35:52.54 <Archive_home> Archive.setRawPosition! 50
@22:35:52.57 <Plunge_home> Plunge.in :default
@22:35:52.66 <Archive_home> Archive.seek :home
@22:35:52.77 <Lysis2_home> Lysis2.in :default
@22:35:52.88 <PinPort_home> PinPort.setRawPosition! 0
@22:35:52.96 PinPort.seek :home
@22:35:53.00 <Archive_home> Archive.in :default
@22:35:53.13 <PinPort_home> PinPort.in :default
@22:35:55.07 <Toroid_home> Toroid.setRawPosition! 0
@22:35:55.13 Toroid.seek :home
@22:35:55.20 Toroid.in :default
@22:35:55.27 <clearToroid> Toroid.seek :clear
@22:35:55.42 <LRAUV> PinPort.in :unlocking
@22:35:55.49 PinPort.seek :clear
@22:35:58.93 Air.seek :clear
@22:35:59.82 Lysis1.seek :clear
@22:36:00.61 Lysis2.seek :clear
@22:36:01.39 Archive.seek :clear
@22:36:02.18 Carousel.in :default
@22:36:02.29 Carousel.home.seek 58
@22:36:02.55 Selecting Cartridge 57
@22:36:02.82 Carousel.seek 57
@22:36:03.01 Slide::Error in LRAUV -- Storage Carousel (SC) positionErr at 58 - 55 counts (sensed 58 - 48 counts)
@22:36:03.03 Retry #1 of 4
@22:36:03.05 Carousel.hold
@22:36:03.17 Carousel.in :default
@22:36:03.23 Carousel.home.seek 57
@22:36:03.78 Lysis1.in :fast
@22:36:03.83 Lysis1.seek :locked
@22:36:06.68 <Plunge> Plunge.seek :locked
@22:36:06.84 <Air> Air.in :fast
@22:36:06.89 Air.seek :locked
@22:36:07.82 <LRAUV> Carousel.reconfigure :locked
@22:36:07.96 Powering cartridge bus
@22:36:10.59 Gate.setIR 1750,5000,0,6
@22:36:10.72 Gate.setIR 0,5000,0,6
@22:36:10.79 PuckHeater.configure :default
@22:36:10.85 Gate.power :heat,:ON,limit to 5000mA
@22:36:11.56 Mounted lysis Cartridge board#J59971
@22:36:11.61 Using default cartridge heat loss curve for HeatingFunctionInCarousel
@22:36:25.87 -> Cmd.startFiltering
@22:36:26.11 <FILTERING> Preparing to filter lyseHoldBS_bac cartridge in slot 57
@22:36:26.34 <sealing> Air.in :default
@22:36:26.45 Air.seek :sealed
@22:36:26.52 <FILTERING> Priming sample loop w/200ml, bypass w/10ml
@22:36:26.53 SamplePump.setPosition! 0ml
@22:36:26.59 Bypass.open
@22:36:28.19 Intake.open
@22:36:29.07 Sea 87.2m deep (143.6psia)
@22:36:29.09 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@22:36:31.70 Vacuum tare is 51% of full scale at 11C (drifted -0%)
@22:36:31.82 <closeIntake> Intake.close
@22:36:31.87 <closeBypass> Bypass.close
@22:36:32.80 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 143.7psia
@22:36:32.82 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@22:36:37.40 Toroid pressure fell 1% to 142.4psia
@22:36:37.41 Bypass.open
@22:36:38.28 Intake.open
@22:36:39.89 Abs.in :default
@22:36:39.95 Abs.hold
@22:36:40.15 SamplePump.in :start
@22:36:40.21 SamplePump.seek 10ml,1:10
@22:36:40.84 <pumpStarted> SamplePump.configure :prime
@22:36:53.02 <FILTERING> Abs.stop
@22:36:53.06 SamplePump.setPosition! 0ml
@22:36:53.12 Exhaust.open
@22:36:54.73 <closeBypass> Bypass.close
@22:36:56.38 <FILTERING> Abs.in :default
@22:36:56.44 Abs.hold
@22:36:56.61 Sea 86.7m deep (142.8psia)
@22:36:56.77 SamplePump.in :start
@22:36:56.84 SamplePump.seek 200ml,13:50
@22:36:57.47 <pumpStarted> SamplePump.configure :prime
@22:41:05.62 <FILTERING> Abs.stop
@22:41:05.69 Bypass.open
@22:41:06.56 Intake.close
@22:41:07.56 Toroid.in :hold
@22:41:07.62 Toroid.seek :crack
@22:41:10.09 Toroid.seek :cartridge
@22:41:12.22 Toroid.in :default
@22:41:12.27 Toroid.seek :clear
@22:41:16.20 <closeExhaust> Exhaust.close
@22:41:16.27 <closeBypass> Bypass.close
@22:41:17.15 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 145.5psia
@22:41:17.17 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@22:41:21.80 Sampling 1000.0ml
@22:41:21.81 SamplePump.setPosition! 0ml
@22:41:21.87 Exhaust.open
@22:41:22.77 Intake.open
@22:41:23.81 Abs.in :default
@22:41:23.86 Abs.hold
@22:41:23.98 Sea 88.2m deep (145.1psia)
@22:41:24.14 SamplePump.in :start
@22:41:24.21 SamplePump.seek 1000ml,1:07:10
@22:41:24.83 <pumpStarted> SamplePump.configure :sample
@22:41:27.22 <sampling> Cartridge 57 filtering 1/1000ml at 0.62ml/s with 29.1psi vacuum 78.2m deep (130.3psia)
@22:42:27.27 Cartridge 57 filtering 35/1000ml at 0.57ml/s with 17.2psi vacuum 83.5m deep (138.1psia)
@22:43:27.27 Cartridge 57 filtering 68/1000ml at 0.54ml/s with 29.2psi vacuum 77.8m deep (129.8psia)
@22:44:27.27 Cartridge 57 filtering 99/1000ml at 0.51ml/s with 24.4psi vacuum 90.1m deep (147.9psia)
@22:45:27.28 Cartridge 57 filtering 129/1000ml at 0.50ml/s with 17.8psi vacuum 95.4m deep (155.7psia)
@22:46:27.27 Cartridge 57 filtering 158/1000ml at 0.48ml/s with 15.9psi vacuum 100.4m deep (163.0psia)
@22:47:27.27 Cartridge 57 filtering 186/1000ml at 0.47ml/s with 26.5psi vacuum 79.0m deep (131.4psia)
@22:48:27.63 Cartridge 57 filtering 214/1000ml at 0.46ml/s with 30.1psi vacuum 90.0m deep (147.7psia)
@22:49:27.28 Cartridge 57 filtering 241/1000ml at 0.45ml/s with 21.6psi vacuum 91.3m deep (149.6psia)
@22:50:27.28 Cartridge 57 filtering 268/1000ml at 0.45ml/s with 22.5psi vacuum 81.3m deep (134.9psia)
@22:51:27.32 Cartridge 57 filtering 294/1000ml at 0.44ml/s with 26.4psi vacuum 94.0m deep (153.7psia)
@22:52:27.27 Cartridge 57 filtering 319/1000ml at 0.43ml/s with 26.8psi vacuum 80.6m deep (133.8psia)
@22:53:27.27 Cartridge 57 filtering 345/1000ml at 0.42ml/s with 29.4psi vacuum 81.7m deep (135.5psia)
@22:54:27.28 Cartridge 57 filtering 370/1000ml at 0.41ml/s with 21.0psi vacuum 97.6m deep (159.0psia)
@22:55:27.27 Cartridge 57 filtering 394/1000ml at 0.41ml/s with 30.2psi vacuum 77.7m deep (129.6psia)
@22:56:27.27 Cartridge 57 filtering 418/1000ml at 0.40ml/s with 19.3psi vacuum 84.7m deep (139.8psia)
@22:57:27.27 Cartridge 57 filtering 442/1000ml at 0.40ml/s with 19.4psi vacuum 85.3m deep (140.8psia)
@22:58:27.28 Cartridge 57 filtering 465/1000ml at 0.39ml/s with 18.5psi vacuum 100.4m deep (163.1psia)
@22:59:27.27 Cartridge 57 filtering 489/1000ml at 0.39ml/s with 15.9psi vacuum 96.3m deep (157.1psia)
@23:00:27.27 Cartridge 57 filtering 511/1000ml at 0.38ml/s with 16.5psi vacuum 97.8m deep (159.3psia)
@23:01:27.27 Cartridge 57 filtering 534/1000ml at 0.37ml/s with 16.6psi vacuum 97.3m deep (158.5psia)
@23:02:27.27 Cartridge 57 filtering 556/1000ml at 0.37ml/s with 19.4psi vacuum 93.8m deep (153.3psia)
@23:03:27.28 Cartridge 57 filtering 578/1000ml at 0.37ml/s with 16.9psi vacuum 95.4m deep (155.7psia)
@23:04:27.27 Cartridge 57 filtering 600/1000ml at 0.36ml/s with 17.7psi vacuum 82.8m deep (137.1psia)
@23:05:27.28 Cartridge 57 filtering 621/1000ml at 0.36ml/s with 15.3psi vacuum 86.2m deep (142.2psia)
@23:06:27.28 Cartridge 57 filtering 643/1000ml at 0.35ml/s with 28.3psi vacuum 83.5m deep (138.1psia)
@23:07:27.27 Cartridge 57 filtering 664/1000ml at 0.35ml/s with 19.4psi vacuum 91.7m deep (150.2psia)
@23:08:27.27 Cartridge 57 filtering 684/1000ml at 0.35ml/s with 29.6psi vacuum 77.4m deep (129.1psia)
@23:09:27.27 Cartridge 57 filtering 705/1000ml at 0.34ml/s with 13.3psi vacuum 107.4m deep (173.4psia)
@23:10:27.32 Cartridge 57 filtering 725/1000ml at 0.34ml/s with 26.9psi vacuum 91.0m deep (149.2psia)
@23:11:27.27 Cartridge 57 filtering 745/1000ml at 0.33ml/s with 28.0psi vacuum 89.1m deep (146.4psia)
@23:12:27.27 Cartridge 57 filtering 765/1000ml at 0.33ml/s with 14.9psi vacuum 101.4m deep (164.5psia)
@23:13:27.27 Cartridge 57 filtering 785/1000ml at 0.33ml/s with 16.2psi vacuum 98.1m deep (159.6psia)
@23:14:27.27 Cartridge 57 filtering 804/1000ml at 0.32ml/s with 17.6psi vacuum 97.5m deep (158.8psia)
@23:15:27.27 Cartridge 57 filtering 823/1000ml at 0.32ml/s with 20.5psi vacuum 95.0m deep (155.1psia)
@23:16:27.28 Cartridge 57 filtering 842/1000ml at 0.32ml/s with 29.6psi vacuum 80.2m deep (133.2psia)
@23:17:27.27 Cartridge 57 filtering 861/1000ml at 0.31ml/s with 16.2psi vacuum 96.0m deep (156.6psia)
@23:18:27.27 Cartridge 57 filtering 880/1000ml at 0.31ml/s with 17.3psi vacuum 96.8m deep (157.7psia)
@23:19:27.27 Cartridge 57 filtering 898/1000ml at 0.31ml/s with 20.1psi vacuum 94.0m deep (153.6psia)
@23:20:27.27 Cartridge 57 filtering 916/1000ml at 0.30ml/s with 28.6psi vacuum 86.4m deep (142.4psia)
@23:21:27.27 Cartridge 57 filtering 934/1000ml at 0.30ml/s with 22.5psi vacuum 88.6m deep (145.7psia)
@23:22:27.27 Cartridge 57 filtering 952/1000ml at 0.30ml/s with 18.2psi vacuum 84.2m deep (139.1psia)
@23:23:27.28 Cartridge 57 filtering 970/1000ml at 0.29ml/s with 20.5psi vacuum 95.5m deep (155.9psia)
@23:24:27.27 Cartridge 57 filtering 987/1000ml at 0.29ml/s with 25.4psi vacuum 84.4m deep (139.4psia)
@23:25:11.25 <FILTERING> Abs.stop
@23:25:11.40 <closeIntake> Intake.close
@23:25:11.45 <closeExhaust> Exhaust.close
@23:25:11.63 <FILTERING> Toroid.in :hold
@23:25:11.69 Toroid.seek :bypass
@23:25:15.28 Toroid.in :default
@23:25:15.35 Toroid.seek :clear
@23:25:18.93 Sampled  1000.0ml
@23:25:20.52 <LRAUV> -> Cmd.startProcessing
@23:25:20.87 <PROCESSING> Handoff.select :waste
@23:25:21.00 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@23:25:27.59 Syringe tare is 51% of full scale at 12C (drifted 0%)
@23:25:27.62 PinPort.in :locking
@23:25:27.68 PinPort.seek :locked
@23:25:30.12 <posArchive> Archive.in :fast
@23:25:30.20 <posLyS2> Lysis2.in :fast
@23:25:30.23 <PROCESSING> Lysis1.seek 1250uL
@23:25:30.31 <posArchive> Archive.seek :empty
@23:25:30.39 <posLyS2> Lysis2.seek :empty
@23:25:38.82 <PROCESSING> Evacuating puck with filter bubble point of 50psi
@23:25:38.84 PinPort.seek :locked
@23:25:38.93 Handoff.select :waste
@23:25:38.95 Plunge.seek :puckBottom
@23:25:40.29 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@23:25:46.86 Air syringe pumping up to 1 strokes to reach 8psi
@23:25:46.88 Air stroke #1 of 1
@23:25:46.97 Air.in :pressurize
@23:25:47.03 Air.seek :pressurized
@23:25:52.10 -0.3psi after pressurize
@23:25:52.12 Air.in :compress
@23:25:52.19 Air.seek :evac
@23:25:59.22 0.9psi after compress
@23:26:10.15 0.1psi after compress (stable)
@23:26:10.17 Air.in :default
@23:26:10.24 Air.hold
@23:26:10.41 at -0.1psi Opened to waste
@23:26:10.43 Handoff.select :blocked
@23:26:10.57 Plunge.seek :puckTop
@23:26:11.52 Air syringe pumping up to 8 strokes to reach 50psi
@23:26:11.53 Air stroke #1 of 8
@23:26:11.63 Air.in :compress
@23:26:11.69 Air.seek :evac
@23:26:11.82 9.4psi after compress
@23:26:22.39 10.0psi after compress (stable)
@23:26:22.41 Air.in :gulp
@23:26:22.48 Air.seek :topVent
@23:26:29.05 10.1psi after gulp
@23:26:34.98 10.1psi after gulp (stable)
@23:26:34.99 Air stroke #2 of 8
@23:26:35.08 Air.in :pressurize
@23:26:35.14 Air.seek :pressurized
@23:26:41.57 9.9psi after pressurize
@23:26:41.59 Air.in :compress
@23:26:41.65 Air.seek :evac
@23:26:48.68 23.8psi after compress
@23:27:01.75 28.5psi after compress (stable)
@23:27:01.77 Air.in :gulp
@23:27:01.85 Air.seek :topVent
@23:27:08.43 28.5psi after gulp
@23:27:14.01 28.6psi after gulp (stable)
@23:27:14.02 Air stroke #3 of 8
@23:27:14.11 Air.in :pressurize
@23:27:14.17 Air.seek :pressurized
@23:27:20.60 28.4psi after pressurize
@23:27:20.61 Air.in :compress
@23:27:20.99 Air.seek :evac
@23:27:28.02 39.3psi after compress
@23:27:41.10 44.2psi after compress (stable)
@23:27:41.12 Air.in :gulp
@23:27:41.18 Air.seek :topVent
@23:27:47.76 44.4psi after gulp
@23:27:53.33 44.6psi after gulp (stable)
@23:27:53.34 Air stroke #4 of 8
@23:27:53.43 Air.in :pressurize
@23:27:53.49 Air.seek :pressurized
@23:27:59.92 44.0psi after pressurize
@23:27:59.93 Air.in :compress
@23:28:00.00 Air.seek :evac
@23:28:06.68 Reached 50.5 of 50.0psi after pumping 7085uL (3.99 strokes)
@23:28:06.69 Air.in :default
@23:28:06.76 Air.hold
@23:28:06.85 Plunge.seek :puckBottom
@23:28:07.80 delay 3 seconds
@23:28:10.82 Handoff.select :waste
@23:28:10.94 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@23:28:26.52 delay 1:00
@23:29:26.54 Handoff.select :blocked
@23:29:26.67 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@23:29:49.25 Handoff.select :waste
@23:29:49.37 1.6psi at evacEnd
@23:29:49.38 Handoff.select :blocked
@23:29:49.52 Plunge.seek :puckTop
@23:29:50.47 Air.in :fast
@23:29:50.53 Air.seek :topVent
@23:29:57.09 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi
@23:30:03.66 Stable @13.8psi after Handoff blocked
@23:30:03.67 Air syringe pumping up to 8 strokes to reach 35psi
@23:30:03.70 Air stroke #1 of 8
@23:30:03.79 Air.in :pressurize
@23:30:03.85 Air.seek :pressurized
@23:30:10.27 13.7psi after pressurize
@23:30:10.29 Air.in :compress
@23:30:10.36 Air.seek :evac
@23:30:17.39 23.6psi after compress
@23:30:28.97 27.2psi after compress (stable)
@23:30:29.00 Air.in :gulp
@23:30:29.07 Air.seek :topVent
@23:30:35.65 27.1psi after gulp
@23:30:41.22 27.4psi after gulp (stable)
@23:30:41.23 Air stroke #2 of 8
@23:30:41.32 Air.in :pressurize
@23:30:41.38 Air.seek :pressurized
@23:30:47.81 27.0psi after pressurize
@23:30:47.83 Air.in :compress
@23:30:47.90 Air.seek :evac
@23:30:54.84 Reached 35.6 of 35.0psi after pumping 3547uL (2.00 strokes)
@23:30:54.86 Air.in :default
@23:30:54.93 Air.hold
@23:30:55.01 Plunge.seek :puckBottom
@23:30:55.96 delay 3 seconds
@23:30:58.98 Handoff.select :waste
@23:30:59.11 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@23:31:08.18 delay 1:00
@23:32:08.21 Handoff.select :blocked
@23:32:08.34 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@23:32:30.91 Handoff.select :waste
@23:32:31.03 1.8psi at evacEnd
@23:32:31.05 Plunge.seek :pTop
@23:32:32.01 delay 5 seconds
@23:32:37.03 Air.in :glide
@23:32:37.09 Air.seek :fullVent
@23:32:38.99 delay 2 seconds
@23:32:41.04 Plunge.seek :pBottom
@23:32:41.98 Seawater evacuated, Pressure above/below filter released.
@23:32:42.00 Plunge.seek :locked
@23:32:43.33 Handoff.select :waste
@23:32:43.42 Lysis1.in :slow
@23:32:43.48 Lysis1.seek 950uL
@23:32:57.64 delay 5 seconds
@23:33:02.66 Handoff.select :blocked
@23:33:02.79 Plunge.seek :puckTop
@23:33:03.67 Lysis1.seek 350uL
@23:33:31.84 delay 3 seconds
@23:33:34.92 Air.in mix with 2.32937 parts Lysis1
@23:33:35.46 <Airmixer> Air.seek :evac
@23:33:35.50 <PROCESSING> Lysis1.seek 190uL
@23:33:43.30 Air.in :fast
@23:33:43.36 Air.seek :full
@23:33:50.05 Lysis1.seek :empty
@23:33:59.18 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @24.4psi
@23:33:59.20 Plunge.seek :puckBottom
@23:34:00.26 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @19.1psi
@23:34:00.28 Polling Can every 2:00
@23:34:00.83 PuckHeater.configure :default #ambient 12.0C
@23:34:00.88 PuckHeater.seek 71.7C
@23:35:35.09 PuckHeater.hold 2:00,71.7C
@23:37:34.74 Polling Can every 2:00
@23:37:35.28 PuckHeater.configure :default #ambient 12.0C
@23:37:35.34 PuckHeater.seek 70.6C
@23:37:35.48 PuckHeater.hold 2:00,70.6C
@23:39:35.19 Polling Can every 2:00
@23:39:35.73 PuckHeater.configure :default #ambient 12.0C
@23:39:35.79 PuckHeater.hold 18:00,69.6C
@23:57:31.76 Polling Can every 2:00
@23:57:32.30 PuckHeater.configure :default #ambient 11.9C
@23:57:32.36 PuckHeater.seek 105.5C
@23:58:36.72 PuckHeater.hold 2:00,105.5C
@00:00:36.29PDT19-Jul-25 Polling Can every 2:00
@00:00:36.83 PuckHeater.configure :default #ambient 11.9C
@00:00:36.90 PuckHeater.seek 104.4C
@00:00:37.04 PuckHeater.hold 2:00,104.4C
@00:02:36.68 Polling Can every 2:00
@00:02:37.22 PuckHeater.hold 2:00,103.4C
@00:04:36.86 delay 5:00
@00:09:36.92 Lysate cooked @17.2psi
@00:09:36.94 Plunge.seek :puckTop
@00:09:38.32 Air syringe pumping up to 5 strokes to reach 40psi
@00:09:38.33 Air stroke #1 of 5
@00:09:38.43 Air.in :pressurize
@00:09:38.49 Air.seek :pressurized
@00:09:44.91 17.1psi after pressurize
@00:09:44.93 Air.in :compress
@00:09:45.00 Air.seek :evac
@00:09:50.43 Reached 41.5 of 40.0psi after pumping 1685uL (0.95 strokes)
@00:09:50.45 Air.in :default
@00:09:50.52 Air.hold
@00:09:50.59 delay 2 seconds
@00:09:52.62 Air.in :fast
@00:09:52.67 Air.seek :full
@00:09:58.98 Plunge.seek :puckBottom
@00:09:59.99 Lysis1.seek 138uL
@00:10:06.58 delay 2 seconds
@00:10:08.60 Plunge.seek :puckTop
@00:10:09.56 Lysis1.seek :empty
@00:10:16.16 Plunge.seek :puckBottom
@00:10:17.32 Lysis1.in :extract
@00:10:17.40 Lysis1.seek 675uL,nil
@00:10:19.73 <pump> Air.in :pressurize
@00:10:19.79 Air.seek :pressurized
@00:10:20.04 Syringe::LysatePlungerStuck in PROCESSING -- at < 251uL during Lysis Syringe #1 (LyS1) move
@00:10:20.07 <PROCESSING> Lysis1.in :default
@00:10:20.15 Lysis1.seek 221uL
@00:10:26.22 <pump> 14.0psi after pressurize
@00:10:26.24 Air.in :compressLysate
@00:10:26.31 Air.seek :evac
@00:10:33.81 Reached 15.6 of 15.5psi after pumping 1495uL (0.84 strokes)
@00:10:33.83 Air.in :default
@00:10:33.89 Air.hold
@00:10:34.02 <PROCESSING> Lysis1.in :extract
@00:10:34.09 Lysis1.seek 675uL,nil
@00:10:35.66 <pump> Air.in :compressLysate
@00:10:35.73 Air.seek :evac
@00:10:40.25 Reached 15.2 of 15.5psi after pumping 1556uL (0.88 strokes)
@00:10:40.26 Air.in :default
@00:10:40.34 Air.hold
@00:10:41.22 Air.in :compressLysate
@00:10:41.27 Air.seek :evac
@00:10:45.83 Reached 15.7 of 15.5psi after pumping 1617uL (0.91 strokes)
@00:10:45.84 Air.in :default
@00:10:45.91 Air.hold
@00:10:46.68 Air.in :compressLysate
@00:10:46.75 Air.seek :evac
@00:10:55.09 Air.in :default
@00:10:55.15 Air.hold
@00:10:55.35 <PROCESSING> Pulled 675uL lysate @14.1psi
@00:10:55.49 Lysis1.seek 800uL,nil
@00:10:55.68 <pump> Air.in :compressLysate
@00:10:55.74 Air.seek :evac
@00:10:59.31 13.2psi after compress
@00:11:09.88 14.1psi after compress (stable)
@00:11:15.06 Air.in :default
@00:11:15.22 Air.hold
@00:11:15.29 <PROCESSING> Lysis1.in :default
@00:11:15.36 Lysis1.hold
@00:11:15.62 Air.in :glide
@00:11:15.68 Air.seek :fullVent
@00:11:17.48 Handoff.select :waste
@00:11:17.60 Recovered 734uL lysate @14.1psi
@00:11:17.62 delay 3 seconds
@00:11:20.70 After 3 delay Recovered 734uL lysate @0.1psi
@00:11:21.83 <LRAUV> -> Cmd.stop
@00:11:21.92 <closeHandoff> Handoff.select :close
@00:11:22.06 <LRAUV> Gate.power :heat,:OFF
@00:11:22.08 Gate.power :cartridge,:OFF
@00:11:22.17 PinPort.in :unlocking
@00:11:22.24 PinPort.seek :clear
@00:11:24.67 Carousel.in :default
@00:11:24.78 Carousel.home.seek 57
@00:11:25.08 Plunge.seek :locked
@00:11:26.46 <Archive> Archive.in :unlocking
@00:11:26.58 Archive.seek :clear
@00:11:26.63 <Air> Air.in :unlocking
@00:11:26.77 Air.seek :clear
@00:11:26.88 <Lysis1> Lysis1.in :unlocking
@00:11:27.02 Lysis1.seek :clear
@00:11:27.06 <Lysis2> Lysis2.in :unlocking
@00:11:27.17 Lysis2.seek :clear
@00:11:31.92 <LRAUV> Gate.power :analytic1,:OFF
@00:11:31.95 Gate.power :analytic2,:OFF
@00:11:31.97 Gate.power :analytic3,:OFF
@00:11:31.99 Gate.power :motor,:OFF
@00:11:32.02 Gate.power :core,:OFF
@00:11:32.05 Gate.power :main,:OFF
@00:11:32.07 Safely stopped and ready to power off
##-- file closed 2025-07-19T07:39:34.662Z

##-- file opened 2025-07-19T07:39:34.674Z
@00:39:34.85 -> showStatus
@00:39:36.89 -> Cmd.loadCartridge -15
@00:39:37.00 Gate.power :main,:ON,limit to 5000mA
@00:39:37.02 Gate.power :analytic1,:ON,limit to 4000mA
@00:39:37.05 Gate.power :analytic2,:ON,limit to 4000mA
@00:39:37.07 Gate.power :analytic3,:ON,limit to 4000mA
@00:39:37.12 Powering core bus
@00:39:39.83 Plunge.configure :default
@00:39:39.91 SamplePump.configure :default
@00:39:39.99 Toroid.configure :default
@00:39:40.07 Carousel.configure :default
@00:39:40.15 Abs.configure :default
@00:39:40.23 Archive.configure :default
@00:39:40.31 Air.configure :default
@00:39:40.36 Lysis1.configure :default
@00:39:40.43 Lysis2.configure :default
@00:39:40.51 PinPort.configure :default
@00:39:40.56 All elf controllers running firmware version 4.92
@00:39:40.59 Gate.power :motor,:ON,limit to 5000mA
@00:39:40.84 <Plunge_home> Plunge.home.jog 0
@00:39:41.33 <PinPort_home> PinPort.home.jog 0
@00:39:41.36 <Lysis2_home> Lysis2.home.jog 0
@00:39:41.39 <Lysis1_home> Lysis1.home.jog 0
@00:39:41.42 <Archive_home> Archive.home.jog 0
@00:39:41.45 <Carousel_home> Carousel.home.jog 0
@00:39:41.48 <Toroid_home> Toroid.home.jog 0
@00:39:41.51 <Air_home> Air.home.jog 0
@00:39:42.51 <Plunge_home> Plunge.in :homing
@00:39:42.69 <Lysis2_home> Lysis2.in :homing
@00:39:42.75 <Air_home> Air.in :homing
@00:39:42.77 <Archive_home> Archive.in :homing
@00:39:42.80 <Plunge_home> Plunge.jog 1587
@00:39:42.83 <PinPort_home> PinPort.in :homing
@00:39:42.87 <Lysis1_home> Lysis1.in :homing
@00:39:42.90 <Toroid_home> Toroid.in :homing
@00:39:43.11 <Lysis2_home> Lysis2.jog 304
@00:39:43.17 <Air_home> Air.jog 360
@00:39:43.21 <Archive_home> Archive.jog 1596
@00:39:43.25 <Lysis1_home> Lysis1.jog 308
@00:39:43.28 <PinPort_home> PinPort.jog -1203
@00:39:43.31 <Toroid_home> Toroid.jog 1281
@00:39:43.95 <Lysis2_home> Lysis2.setRawPosition! 50
@00:39:44.02 Lysis2.seek :home
@00:39:44.13 <Lysis1_home> Lysis1.setRawPosition! 50
@00:39:44.15 <Air_home> Air.setRawPosition! 0
@00:39:44.23 <Lysis1_home> Lysis1.seek :home
@00:39:44.27 <Air_home> Air.seek :home
@00:39:44.42 <Lysis2_home> Lysis2.in :default
@00:39:44.45 <Plunge_home> Plunge.setRawPosition! 50
@00:39:44.55 Plunge.seek :home
@00:39:44.66 <Lysis1_home> Lysis1.in :default
@00:39:44.70 <Air_home> Air.in :default
@00:39:44.80 <Archive_home> Archive.setRawPosition! 50
@00:39:44.86 Archive.seek :home
@00:39:44.95 <Plunge_home> Plunge.in :default
@00:39:45.15 <Archive_home> Archive.in :default
@00:39:45.22 <PinPort_home> PinPort.setRawPosition! 0
@00:39:45.28 PinPort.seek :home
@00:39:45.38 PinPort.in :default
@00:39:47.72 <Toroid_home> Toroid.setRawPosition! 0
@00:39:47.78 Toroid.seek :home
@00:39:47.85 Toroid.in :default
@00:39:47.92 <clearToroid> Toroid.seek :clear
@00:39:48.06 <LRAUV> PinPort.in :unlocking
@00:39:48.13 PinPort.seek :clear
@00:39:51.59 Air.seek :clear
@00:39:52.48 Lysis1.seek :clear
@00:39:53.28 Lysis2.seek :clear
@00:39:54.06 Archive.seek :clear
@00:39:54.84 Carousel.in :default
@00:39:54.95 Carousel.home.seek 57
@00:39:55.21 Selecting Cartridge 40
@00:39:55.47 Carousel.seek 40
@00:40:00.37 Lysis1.in :fast
@00:40:00.43 Lysis1.seek :locked
@00:40:02.92 <Plunge> Plunge.seek :locked
@00:40:03.06 <Air> Air.in :fast
@00:40:03.12 Air.seek :locked
@00:40:04.04 <LRAUV> Carousel.reconfigure :locked
@00:40:04.19 Powering cartridge bus
@00:40:06.86 Gate.setIR 1750,5000,0,6
@00:40:06.98 Gate.setIR 0,5000,0,6
@00:40:07.06 PuckHeater.configure :default
@00:40:07.12 Gate.power :heat,:ON,limit to 5000mA
@00:40:07.83 Mounted lysis Cartridge board#J59999
@00:40:07.87 Using default cartridge heat loss curve for HeatingFunctionInCarousel
@00:40:16.12 -> Cmd.startFiltering
@00:40:16.35 <FILTERING> Preparing to filter lyseHoldBS_bac cartridge in slot 40
@00:40:16.59 <sealing> Air.in :default
@00:40:16.72 Air.seek :sealed
@00:40:16.74 <FILTERING> Priming sample loop w/200ml, bypass w/10ml
@00:40:16.79 SamplePump.setPosition! 0ml
@00:40:16.86 Bypass.open
@00:40:18.48 Intake.open
@00:40:19.41 Sea 42.8m deep (78.0psia)
@00:40:19.43 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@00:40:22.03 Vacuum tare is 51% of full scale at 11C (drifted -0%)
@00:40:22.17 <closeIntake> Intake.close
@00:40:22.22 <closeBypass> Bypass.close
@00:40:23.10 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 77.8psia
@00:40:23.13 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@00:40:27.71 Toroid pressure rose 2% to 79.1psia
@00:40:27.72 Bypass.open
@00:40:28.55 Intake.open
@00:40:30.11 Abs.in :default
@00:40:30.16 Abs.hold
@00:40:30.38 SamplePump.in :start
@00:40:30.44 SamplePump.seek 10ml,1:10
@00:40:31.08 <pumpStarted> SamplePump.configure :prime
@00:40:43.26 <FILTERING> Abs.stop
@00:40:43.32 SamplePump.setPosition! 0ml
@00:40:43.37 Exhaust.open
@00:40:44.99 <closeBypass> Bypass.close
@00:40:46.62 <FILTERING> Abs.in :default
@00:40:46.68 Abs.hold
@00:40:47.15 Sea 44.3m deep (80.2psia)
@00:40:47.33 SamplePump.in :start
@00:40:47.39 SamplePump.seek 200ml,13:50
@00:40:48.02 <pumpStarted> SamplePump.configure :prime
@00:44:56.15 <FILTERING> Abs.stop
@00:44:56.23 Bypass.open
@00:44:57.09 Intake.close
@00:44:58.08 Toroid.in :hold
@00:44:58.14 Toroid.seek :crack
@00:45:00.61 Toroid.seek :cartridge
@00:45:02.73 Toroid.in :default
@00:45:02.79 Toroid.seek :clear
@00:45:06.73 <closeExhaust> Exhaust.close
@00:45:06.77 <closeBypass> Bypass.close
@00:45:07.72 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 77.3psia
@00:45:07.74 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@00:45:12.33 Cartridge pressure fell 1% to 76.6psia
@00:45:12.41 Sampling 1000.0ml
@00:45:12.43 SamplePump.setPosition! 0ml
@00:45:12.49 Exhaust.open
@00:45:13.40 Intake.open
@00:45:14.43 Abs.in :default
@00:45:14.49 Abs.hold
@00:45:14.61 Sea 42.4m deep (77.3psia)
@00:45:14.78 SamplePump.in :start
@00:45:14.85 SamplePump.seek 1000ml,1:07:10
@00:45:15.47 <pumpStarted> SamplePump.configure :sample
@00:45:17.86 <sampling> Cartridge 40 filtering 2/1000ml at 0.61ml/s with 17.5psi vacuum 47.3m deep (84.6psia)
@00:46:17.90 Cartridge 40 filtering 36/1000ml at 0.57ml/s with 18.2psi vacuum 53.8m deep (94.2psia)
@00:47:17.90 Cartridge 40 filtering 69/1000ml at 0.55ml/s with 21.4psi vacuum 42.5m deep (77.5psia)
@00:48:17.90 Cartridge 40 filtering 101/1000ml at 0.54ml/s with 26.4psi vacuum 38.2m deep (71.2psia)
@00:49:17.90 Cartridge 40 filtering 133/1000ml at 0.53ml/s with 20.2psi vacuum 38.7m deep (71.8psia)
@00:50:17.90 Cartridge 40 filtering 163/1000ml at 0.51ml/s with 15.7psi vacuum 57.8m deep (100.1psia)
@00:51:17.95 Cartridge 40 filtering 194/1000ml at 0.51ml/s with 24.6psi vacuum 48.2m deep (85.9psia)
@00:52:17.90 Cartridge 40 filtering 224/1000ml at 0.50ml/s with 21.3psi vacuum 48.3m deep (86.1psia)
@00:53:17.90 Cartridge 40 filtering 253/1000ml at 0.49ml/s with 21.8psi vacuum 43.8m deep (79.4psia)
@00:54:18.27 Cartridge 40 filtering 282/1000ml at 0.48ml/s with 24.2psi vacuum 34.8m deep (66.1psia)
@00:55:17.90 Cartridge 40 filtering 311/1000ml at 0.48ml/s with 21.6psi vacuum 48.1m deep (85.7psia)
@00:56:17.90 Cartridge 40 filtering 339/1000ml at 0.47ml/s with 17.6psi vacuum 48.4m deep (86.2psia)
@00:57:17.90 Cartridge 40 filtering 367/1000ml at 0.46ml/s with 22.6psi vacuum 50.5m deep (89.3psia)
@00:58:17.90 Cartridge 40 filtering 395/1000ml at 0.46ml/s with 20.6psi vacuum 45.6m deep (82.1psia)
@00:59:17.91 Cartridge 40 filtering 422/1000ml at 0.46ml/s with 23.8psi vacuum 45.3m deep (81.6psia)
@01:00:17.90 Cartridge 40 filtering 449/1000ml at 0.45ml/s with 23.5psi vacuum 33.4m deep (64.0psia)
@01:01:17.90 Cartridge 40 filtering 476/1000ml at 0.44ml/s with 27.4psi vacuum 37.5m deep (70.1psia)
@01:02:17.90 Cartridge 40 filtering 502/1000ml at 0.44ml/s with 24.4psi vacuum 37.9m deep (70.7psia)
@01:03:17.90 Cartridge 40 filtering 528/1000ml at 0.44ml/s with 32.2psi vacuum 26.3m deep (53.6psia)
@01:04:17.90 Cartridge 40 filtering 554/1000ml at 0.43ml/s with 21.6psi vacuum 42.6m deep (77.7psia)
@01:05:17.90 Cartridge 40 filtering 580/1000ml at 0.43ml/s with 18.9psi vacuum 50.6m deep (89.4psia)
@01:06:17.90 Cartridge 40 filtering 605/1000ml at 0.42ml/s with 14.5psi vacuum 52.8m deep (92.7psia)
@01:07:17.90 Cartridge 40 filtering 630/1000ml at 0.42ml/s with 21.5psi vacuum 43.5m deep (78.9psia)
@01:08:17.90 Cartridge 40 filtering 655/1000ml at 0.41ml/s with 30.7psi vacuum 34.3m deep (65.4psia)
@01:09:17.90 Cartridge 40 filtering 679/1000ml at 0.41ml/s with 31.8psi vacuum 42.3m deep (77.3psia)
@01:10:17.90 Cartridge 40 filtering 703/1000ml at 0.40ml/s with 29.2psi vacuum 30.9m deep (60.3psia)
@01:11:17.90 Cartridge 40 filtering 727/1000ml at 0.40ml/s with 19.7psi vacuum 41.1m deep (75.4psia)
@01:12:17.90 Cartridge 40 filtering 751/1000ml at 0.40ml/s with 17.8psi vacuum 46.4m deep (83.3psia)
@01:13:17.91 Cartridge 40 filtering 775/1000ml at 0.39ml/s with 29.0psi vacuum 28.2m deep (56.3psia)
@01:14:17.90 Cartridge 40 filtering 798/1000ml at 0.39ml/s with 19.7psi vacuum 46.5m deep (83.4psia)
@01:15:17.90 Cartridge 40 filtering 822/1000ml at 0.39ml/s with 28.1psi vacuum 30.5m deep (59.7psia)
@01:16:17.90 Cartridge 40 filtering 845/1000ml at 0.38ml/s with 28.0psi vacuum 32.9m deep (63.3psia)
@01:17:17.90 Cartridge 40 filtering 867/1000ml at 0.38ml/s with 10.1psi vacuum 62.3m deep (106.7psia)
@01:18:17.91 Cartridge 40 filtering 890/1000ml at 0.38ml/s with 14.9psi vacuum 49.6m deep (87.9psia)
@01:19:17.90 Cartridge 40 filtering 912/1000ml at 0.37ml/s with 26.5psi vacuum 46.6m deep (83.6psia)
@01:20:17.90 Cartridge 40 filtering 934/1000ml at 0.37ml/s with 13.5psi vacuum 53.0m deep (93.1psia)
@01:21:17.90 Cartridge 40 filtering 956/1000ml at 0.37ml/s with 21.6psi vacuum 45.8m deep (82.4psia)
@01:22:17.90 Cartridge 40 filtering 978/1000ml at 0.36ml/s with 14.1psi vacuum 55.7m deep (97.1psia)
@01:23:17.90 Cartridge 40 filtering 999/1000ml at 0.36ml/s with 29.8psi vacuum 38.5m deep (71.6psia)
@01:23:19.39 <FILTERING> Abs.stop
@01:23:19.46 <closeIntake> Intake.close
@01:23:19.51 <closeExhaust> Exhaust.close
@01:23:19.70 <FILTERING> Toroid.in :hold
@01:23:19.76 Toroid.seek :bypass
@01:23:23.36 Toroid.in :default
@01:23:23.42 Toroid.seek :clear
@01:23:27.00 Sampled  1000.0ml
@01:23:28.61 <LRAUV> -> Cmd.startProcessing
@01:23:29.35 <PROCESSING> Handoff.select :waste
@01:23:29.48 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@01:23:36.08 Syringe tare is 51% of full scale at 12C (drifted 1%)
@01:23:36.11 PinPort.in :locking
@01:23:36.17 PinPort.seek :locked
@01:23:38.62 <posArchive> Archive.in :fast
@01:23:38.66 <posLyS2> Lysis2.in :fast
@01:23:38.75 <PROCESSING> Lysis1.seek 1250uL
@01:23:38.84 <posArchive> Archive.seek :empty
@01:23:38.88 <posLyS2> Lysis2.seek :empty
@01:23:47.32 <PROCESSING> Evacuating puck with filter bubble point of 50psi
@01:23:47.34 PinPort.seek :locked
@01:23:47.43 Handoff.select :waste
@01:23:47.46 Plunge.seek :puckBottom
@01:23:48.79 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@01:23:55.37 Air syringe pumping up to 1 strokes to reach 8psi
@01:23:55.38 Air stroke #1 of 1
@01:23:55.48 Air.in :pressurize
@01:23:55.54 Air.seek :pressurized
@01:24:00.61 -0.4psi after pressurize
@01:24:00.63 Air.in :compress
@01:24:00.70 Air.seek :evac
@01:24:07.73 0.7psi after compress
@01:24:18.80 -0.1psi after compress (stable)
@01:24:18.82 Air.in :default
@01:24:18.88 Air.hold
@01:24:19.07 at -0.1psi Opened to waste
@01:24:19.08 Handoff.select :blocked
@01:24:19.25 Plunge.seek :puckTop
@01:24:20.21 Air syringe pumping up to 8 strokes to reach 50psi
@01:24:20.22 Air stroke #1 of 8
@01:24:20.32 Air.in :compress
@01:24:20.38 Air.seek :evac
@01:24:20.51 9.2psi after compress
@01:24:31.08 10.0psi after compress (stable)
@01:24:31.10 Air.in :gulp
@01:24:31.17 Air.seek :topVent
@01:24:37.75 9.8psi after gulp
@01:24:43.32 10.2psi after gulp (stable)
@01:24:43.33 Air stroke #2 of 8
@01:24:43.43 Air.in :pressurize
@01:24:43.49 Air.seek :pressurized
@01:24:50.26 10.1psi after pressurize
@01:24:50.28 Air.in :compress
@01:24:50.35 Air.seek :evac
@01:24:57.37 23.9psi after compress
@01:25:09.45 27.6psi after compress (stable)
@01:25:09.47 Air.in :gulp
@01:25:09.54 Air.seek :topVent
@01:25:16.14 27.6psi after gulp
@01:25:21.71 27.9psi after gulp (stable)
@01:25:21.72 Air stroke #3 of 8
@01:25:21.81 Air.in :pressurize
@01:25:21.87 Air.seek :pressurized
@01:25:28.30 27.4psi after pressurize
@01:25:28.32 Air.in :compress
@01:25:28.39 Air.seek :evac
@01:25:35.42 39.6psi after compress
@01:25:47.99 43.4psi after compress (stable)
@01:25:48.01 Air.in :gulp
@01:25:48.09 Air.seek :topVent
@01:25:54.67 43.6psi after gulp
@01:26:00.25 43.5psi after gulp (stable)
@01:26:00.26 Air stroke #4 of 8
@01:26:00.36 Air.in :pressurize
@01:26:00.42 Air.seek :pressurized
@01:26:06.85 43.2psi after pressurize
@01:26:06.87 Air.in :compress
@01:26:06.94 Air.seek :evac
@01:26:13.62 Reached 50.6 of 50.0psi after pumping 7084uL (3.99 strokes)
@01:26:13.64 Air.in :default
@01:26:13.69 Air.hold
@01:26:13.78 Plunge.seek :puckBottom
@01:26:14.73 delay 3 seconds
@01:26:17.75 Handoff.select :waste
@01:26:17.90 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@01:26:39.48 delay 1:00
@01:27:39.50 Handoff.select :blocked
@01:27:39.63 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@01:28:02.20 Handoff.select :waste
@01:28:02.33 1.3psi at evacEnd
@01:28:02.34 Handoff.select :blocked
@01:28:02.48 Plunge.seek :puckTop
@01:28:03.43 Air.in :fast
@01:28:03.50 Air.seek :topVent
@01:28:10.06 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi
@01:28:16.63 Stable @15.5psi after Handoff blocked
@01:28:16.65 Air syringe pumping up to 8 strokes to reach 35psi
@01:28:16.67 Air stroke #1 of 8
@01:28:16.77 Air.in :pressurize
@01:28:16.83 Air.seek :pressurized
@01:28:23.26 15.3psi after pressurize
@01:28:23.28 Air.in :compress
@01:28:23.35 Air.seek :evac
@01:28:30.37 25.5psi after compress
@01:28:41.96 28.5psi after compress (stable)
@01:28:41.98 Air.in :gulp
@01:28:42.05 Air.seek :topVent
@01:28:48.64 28.4psi after gulp
@01:28:54.25 28.8psi after gulp (stable)
@01:28:54.26 Air stroke #2 of 8
@01:28:54.36 Air.in :pressurize
@01:28:54.42 Air.seek :pressurized
@01:29:00.85 28.6psi after pressurize
@01:29:00.87 Air.in :compress
@01:29:00.94 Air.seek :evac
@01:29:07.49 Reached 35.7 of 35.0psi after pumping 3525uL (1.98 strokes)
@01:29:07.51 Air.in :default
@01:29:07.57 Air.hold
@01:29:07.65 Plunge.seek :puckBottom
@01:29:08.61 delay 3 seconds
@01:29:11.63 Handoff.select :waste
@01:29:11.76 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@01:29:18.33 delay 1:00
@01:30:18.36 Handoff.select :blocked
@01:30:18.49 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@01:30:41.07 Handoff.select :waste
@01:30:41.20 1.9psi at evacEnd
@01:30:41.22 Plunge.seek :pTop
@01:30:42.18 delay 5 seconds
@01:30:47.20 Air.in :glide
@01:30:47.25 Air.seek :fullVent
@01:30:49.40 delay 2 seconds
@01:30:51.42 Plunge.seek :pBottom
@01:30:52.37 Seawater evacuated, Pressure above/below filter released.
@01:30:52.39 Plunge.seek :locked
@01:30:53.71 Handoff.select :waste
@01:30:53.81 Lysis1.in :slow
@01:30:53.87 Lysis1.seek 950uL
@01:31:08.03 delay 5 seconds
@01:31:13.06 Handoff.select :blocked
@01:31:13.19 Plunge.seek :puckTop
@01:31:14.07 Lysis1.seek 350uL
@01:31:42.26 delay 3 seconds
@01:31:45.35 Air.in mix with 2.32937 parts Lysis1
@01:31:45.87 <Airmixer> Air.seek :evac
@01:31:45.93 <PROCESSING> Lysis1.seek 190uL
@01:31:53.68 Air.in :fast
@01:31:53.75 Air.seek :full
@01:32:00.39 Lysis1.seek :empty
@01:32:09.52 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @16.0psi
@01:32:09.54 Plunge.seek :puckBottom
@01:32:10.60 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @14.6psi
@01:32:10.62 Polling Can every 2:00
@01:32:11.16 PuckHeater.configure :default #ambient 12.3C
@01:32:15.22 I2C::SoftErr in PROCESSING -- Bus Error writing to Cartridge 
@01:32:15.24 Retry #1 of 3
@01:32:19.29 I2C::SoftErr in PROCESSING -- Bus Error writing to Cartridge 
@01:32:19.31 Retry #2 of 3
@01:32:23.37 I2C::SoftErr in PROCESSING -- Bus Error writing to Cartridge 
@01:32:23.39 Retry #3 of 3
@01:32:31.48 I2C::SoftErr in PROCESSING -- Bus Error writing to Cartridge 
@01:32:31.50 Retry #1 of 3
@01:32:35.56 I2C::SoftErr in PROCESSING -- Bus Error writing to Cartridge 
@01:32:35.58 Retry #2 of 3
@01:32:39.64 I2C::SoftErr in PROCESSING -- Bus Error writing to Cartridge 
@01:32:39.66 Retry #3 of 3
@01:32:43.75 lysing strike 1 of 3
@01:32:43.78 I2C::SoftErr in PROCESSING -- Bus Error writing to Cartridge  while reading Puck Heater's status (MASKED)
@02:23:28.67 <LRAUV> -> Cmd.stop
@02:23:28.78 Gate.power :heat,:OFF
@02:23:28.80 Gate.power :cartridge,:OFF
@02:23:28.89 PinPort.in :unlocking
@02:23:28.95 PinPort.seek :clear
@02:23:31.39 Carousel.in :default
@02:23:31.50 Carousel.home.seek 40
@02:23:31.82 Plunge.seek :locked
@02:23:33.21 <Archive> Archive.in :unlocking
@02:23:33.34 Archive.seek :clear
@02:23:33.40 <Air> Air.in :unlocking
@02:23:33.55 Air.seek :clear
@02:23:33.60 <Lysis1> Lysis1.in :unlocking
@02:23:33.75 Lysis1.seek :clear
@02:23:33.84 <Lysis2> Lysis2.in :unlocking
@02:23:33.91 Lysis2.seek :clear
@02:23:38.65 <LRAUV> Gate.power :analytic1,:OFF
@02:23:38.67 Gate.power :analytic2,:OFF
@02:23:38.70 Gate.power :analytic3,:OFF
@02:23:38.72 Gate.power :motor,:OFF
@02:23:38.75 Gate.power :core,:OFF
@02:23:38.78 Gate.power :main,:OFF
@02:23:38.80 Safely stopped and ready to power off
##-- file closed 2025-07-19T17:35:18.267Z